design description
DNA affinity purification sequencing (DAP-seq) was performed to map genome-wide binding sites of the transcription factor SmWRKY17 in Salvia miltiorrhiza. Genomic DNA was extracted from wild-type Salvia miltiorrhiza hairy roots and fragmented. Halo-tagged SmWRKY17 recombinant protein was expressed in vitro and incubated with fragmented genomic DNA. DNA fragments specifically bound to SmWRKY17 were enriched, purified and constructed into Illumina paired-end sequencing libraries.