title: PPARG_OE
organism: Mus musculus
description: Protocols: T helper type 2 cells were extracted from mouse spleens and activated with CD3/CD28. Each mouse replicate is a separate purification of T cells from a collection of mice. Mice were 8-12 weeks old and of mixed gender. Cells were spinoculated with retroviruses overexpressing a gene of interest on day 1. IL4 is 10ng/ml, and the cells were in these for the remainder of the time spent in culture. For the overexpression for chipseq and rnaseq you can refer to Addgene plasmids #117263-117267. Further information can be found in the preprint here: https://www.biorxiv.org/content/early/2017/12/28/196022 or E-MTAB-6285. Lrrc40dec is a truncated version of Lrrc40. On day 4, BFP-positive (infected) cells were FACS sorted and measured by ChIPmentation. The antibody is 3xFLAG, SIGMA clone F1804 DNA was extracted as part of the ChIPmentation protocol Libraries were made as part of the ChIPmentation protocol
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-11-23T17:03:08Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-09-20T10:14:02Z
attributes.tag: External Id
attributes.value: SAMEA4925804
attributes.tag: INSDC center name
attributes.value: Sanger Institute / Karolinska Institutet
attributes.tag: INSDC first public
attributes.value: 2018-11-23T17:03:08Z
attributes.tag: INSDC last update
attributes.value: 2018-09-20T10:14:02Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7258:PPARG_OE
attributes.tag: age
attributes.value: 8 to 12
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: T-helper 2 cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: genotype
attributes.value: MSCV-Pparg-3xFLAG-IRES-BFP
attributes.tag: organism part
attributes.value: spleen
attributes.tag: phenotype
attributes.value: Pparg overexpression
attributes.tag: sample name
attributes.value: E-MTAB-7258:PPARG_OE
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: mixed
attributes.tag: stimulus
attributes.value: CD3/CD28
attributes.tag: strain
attributes.value: CBLA