title: China 50
organism: soil metagenome
description: rice field soil developed after diking, cultivated with rice for 50 years
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2011-07-14T14:45:04Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-09T10:03:16Z
attributes.tag: External Id
attributes.value: SAMEA665732
attributes.tag: Geographic location (country:region,area)
attributes.value: China: Province of Zhejiang
attributes.tag: INSDC center name
attributes.value: MPI-M
attributes.tag: INSDC first public
attributes.value: 2011-07-14T14:45:04Z
attributes.tag: INSDC last update
attributes.value: 2018-03-09T10:03:16Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: China50
attributes.tag: collection date
attributes.value: Spring 2009
attributes.tag: depth
attributes.value: 0-30 cm
attributes.tag: elevation
attributes.value: 0 m.a.s.
attributes.tag: enviornmental package
attributes.value: soil
attributes.tag: environment (biome)
attributes.value: Baily biome: Subtropical devision
attributes.tag: environment (feature)
attributes.value: soil
attributes.tag: environment (material)
attributes.value: paddy field
attributes.tag: geographic location (latitudeandlongitude)
attributes.value: 30.18 N 121.35 E
attributes.tag: history/flooding
attributes.value: seasonally flooded
attributes.tag: investigation type
attributes.value: MIENS_Survey
attributes.tag: pcr primers
attributes.value: Forward primer A189f: 5'- GGN GAC TGG GAC TTC TGG -3' and reverse primer A682r: 5'- GAA SGC NGA GAA GAA SGC -3' or forward primer A189f: 5'- GGN GAC TGG GAC TTC TGG -3' and reverse primer mb661: 5'- CCG GMG CAA CGT CYT TAC C -3'
attributes.tag: ph
attributes.value: 6.9
attributes.tag: project name
attributes.value: 0
attributes.tag: sample name
attributes.value: China50
attributes.tag: scientific_name
attributes.value: soil metagenome
attributes.tag: sequencing method
attributes.value: Pyrosequencing
attributes.tag: soil taxonomic/fao classification
attributes.value: stagnic gleyic cambisol
attributes.tag: target gene
attributes.value: pmoA
attributes.tag: total nitrogen
attributes.value: 1.42 g/kg soil
attributes.tag: total organic carbon
attributes.value: 15.27 g/kg soil