title: HeLa cells transfected with 1 nM antagomir against miR-16
organism: Homo sapiens
description: Total small RNAs prepared from HeLa cells 48h post transfection of  1 nM antagomir miR-16with Dharmafect4 (Dharmacon). Total RNA was extracted using Trizol (Invitrogen), then short (18-29 nt) RNAs were gel-purified. Library was constructed as described in Seitz et al. (Curr. Biol. 18:147-151 (2008)