title: P63a_rep1_614EGAAXX_4_1208
organism: Mus musculus
description: Protocols: Palatal shelves dissected from E13.5/E14.5 mice were cross-linked with 1% formaldehyde for 20 minutes. After reverse cross-link and protein digestion with Proteinase K (Roche), DNA was purified using Qiaquick, PCR purification kit (Qiagen) following the manufacturer instructions. Chromatin was prepared by homogenising the tissue pellet in PBS using an Ultra-Turrax Homogeniser, after which the palatal cells were incubated in PIPES buffer according to the Upstate ChIP assay protocol (Merck Millipore). Chromatin was sonicated using a Sonics Vibracell with seven 10 second pulses set to an amplitude of 40. Chromatin was initially prewashed with Protein A-Agarose beads (SC-2001; Santa Cruz,) and subsequently incubated with 2 μg of α isoform-specific p63 antibody (H129; Santa Cruz) overnight at 4 degree C. Antibody-bound chromatin was recovered using protein A-agarose beads (Santa Cruz).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2015-12-17T13:48:31Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T22:39:40Z
attributes.tag: External Id
attributes.value: SAMEA3177006
attributes.tag: INSDC center name
attributes.value: University of Manchester
attributes.tag: INSDC first public
attributes.value: 2015-12-17T13:48:31Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T22:39:40Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-3179:P63a_rep1_614EGAAXX_4_1208
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: E13.5/E14.5
attributes.tag: organism part
attributes.value: palatal shelves
attributes.tag: sample name
attributes.value: E-MTAB-3179:P63a_rep1_614EGAAXX_4_1208
attributes.tag: scientific_name
attributes.value: Mus musculus