title: TIA1_iCLAP_LUd2_AC
organism: Homo sapiens
description: Protocols: HeLa cells grown in a 10 cm plate. Medium was replaced with ice-cold PBS buffer and cells were subjected to UV-C irradiation (100 mJ/cm2, Stratalinker 2400). Cells were then precipitated by centrifugation for 1 min at 14000 rpm and snap-frozen on dry ice. iCLIP or iCLAP protocol was followed to isolate RNA bound to TIA1 and TIAL1 proteins and amplify cDNA library as described in the manuscript. Specific barcodes were used for each RT reaction, and are specified below for each sequencing run. Random barcodes were also incoperated into cDNAs to distinguish between PCR duplication of cDNA products. The final products were obtained by PCR with Illumina pair-end sequencing primers: 5-CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT-3; 5-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT-3
attributes.tag: ENA first public
attributes.value: 2011-07-29
attributes.tag: ENA last update
attributes.value: 2018-03-08
attributes.tag: External Id
attributes.value: SAMEA680926
attributes.tag: INSDC center alias
attributes.value: CRI
attributes.tag: INSDC center name
attributes.value: Cancer Research UK Cambridge Research Institute
attributes.tag: INSDC first public
attributes.value: 2011-07-29T17:00:40Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T15:27:56Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-432:TIA1_iCLAP_LUd2_AC
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: HeLa
attributes.tag: common name
attributes.value: human
attributes.tag: sample name
attributes.value: E-MTAB-432:TIA1_iCLAP_LUd2_AC
attributes.tag: sex
attributes.value: female