organism: Penaeus vannamei
description: To enable the identification and development of genome-wide Type I SNPs, high-quality total RNA was extracted from the uropod tissue of 30 L. vannamei individuals representing prominent industry domesticated lines, using TRIZOL Reagent (Life Technologies). RNA from each individual were pooled together in equimolar amounts before being converted to double stranded cDNA using the Mint cDNA synthesis kit (Evrogen), and normalised using the Trimmer cDNA normalisation kit (Evrogen) using manufacturers recommendations. Normalised cDNA was then sequenced using an Illumina GA-IIX at 76 bp paired-end resulting in approximately 25 gigabases of paired-end EST sequence data (10x genome coverage).
attributes.tag: isolate
attributes.value: Not applicable
attributes.tag: breed
attributes.value: Not applicable
attributes.tag: host
attributes.value: Not applicable
attributes.tag: isolation_source
attributes.value: Not applicable
attributes.tag: collection_date
attributes.value: 2011-03-09
attributes.tag: geo_loc_name
attributes.value: Indonesia
attributes.tag: tissue
attributes.value: Uropod
attributes.tag: BioSampleModel
attributes.value: Invertebrate