title: Harvested RNA from immortalised human mammary epithelial (HMLE) cells before and after they had undergone EMT and after EMT with QKI knocked down by siRNAs
organism: Homo sapiens
description: To assess whether circRNA production is regulated in EMT, we harvested RNA from immortalised human mammary epithelial (HMLE) cells before and after they had undergone EMT in response to treatment with TGF-β. The TGF-β-treated cells are stably mesenchymal with typical morphology and marker expression and are referred to as mesHMLE cells. RNA from two biological replicates of HMLE and mesHMLE cells as well as two biological replicates of mesHMLE cells with QKI stably knocked down by QKI specific siRNAs was subjected to deep sequencing, using library preparation procedures and bioinformatics pipelines designed to detect circRNAs.
attributes.tag: ENA first public
attributes.value: 2015-03-16
attributes.tag: ENA last update
attributes.value: 2015-01-16
attributes.tag: External Id
attributes.value: SAMEA3209360
attributes.tag: INSDC center alias
attributes.value: CENTRE FOR CANCER BIOLOGY
attributes.tag: INSDC center name
attributes.value: CENTRE FOR CANCER BIOLOGY
attributes.tag: INSDC first public
attributes.value: 2015-03-16T17:04:53Z
attributes.tag: INSDC last update
attributes.value: 2015-01-16T00:14:29Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: MsiQKI_polyAminus_rep2
attributes.tag: common name
attributes.value: human
attributes.tag: sample name
attributes.value: MsiQKI_polyAminus_rep2