organism: Bacillus subtilis
description: sgRNA guide sequences PCR amplified from genomic DNA preparation of mismatch-CRISPRi pooled-growth screen
attributes.tag: strain
attributes.value: strain 168
attributes.tag: isolation_source
attributes.value: Lab isolate
attributes.tag: collection_date
attributes.value: 2019-06-19
attributes.tag: geo_loc_name
attributes.value: USA: University of California, San Francisco, Mission Bay Campus
attributes.tag: sample_type
attributes.value: single-locus amplicons from pooled bacterial culture
attributes.tag: id_info
attributes.value: [large v2 essential-gene library; B. subtilis; 1O-doubling sample for replicate za]
attributes.tag: BioSampleModel
attributes.value: Microbe, viral or environmental