title: Sample 1
organism: Lilium longiflorum
description: Not vernalized (control) Protocols: L. longiflorum bulbs (cultivar White Heaven) were obtained from a nursery at the end of the growing season (August). Bulbs were sanitized and stored in humid standard pot medium at 25C (control, 0W) or at 4C for 2, 5, 7 or 9 weeks (2W, 5W, 7W, 9W, respectively). At each of these time points, shoot apical meristems were excised from the bulbs, immediately frozen in liquid nitrogen and then stored at -80C until RNA extraction. RNA was isolated using the AurumTM Total RNA mini kit (Biorad) following manufacturers instructions. The procedure was followed by an additional DNAse treatment with TURBO DNase (Life technologies) to remove all genomic DNA still present after the isolation. The RNA integrity was confirmed using the 2100 Bioanalyzer (Agilent Technologies). The samples were prepared for transcriptome sequencing using the Illumina kit (TruSeq RNA Sample Preparation Kit v2) following the manufacturers recommendations.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2015-03-17T17:02:51Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T19:39:38Z
attributes.tag: External Id
attributes.value: SAMEA2697751
attributes.tag: INSDC center name
attributes.value: Genetwister Technologies B.V.
attributes.tag: INSDC first public
attributes.value: 2015-03-17T17:02:51Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T19:39:38Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-2825:Sample 1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: trumpet lily
attributes.tag: organism part
attributes.value: apical shoot meristem
attributes.tag: sample name
attributes.value: E-MTAB-2825:Sample 1
attributes.tag: scientific_name
attributes.value: Lilium longiflorum