title: 1072N0009
organism: Homo sapiens
description: Protocols: Plasma was fractionated from blood and cryopreserved (-80°C). To control for the bias of miRNAs from red blood cells in differential analysis, we compiled pools so that average levels of hemolysis were comparable across groups. After exclusion of hemolysis specimens, plasma pools were formed by combining equal volumes of patients' plasma. Total RNA was extracted from 2.5 ml plasma using the miRVana PARIS technology kit (Life Technologies). NGS cDNA libraries were prepared and validated from plasma RNA by ARK Genomics (University of Edinburgh, UK), following manufacturer instructions, with specific barcodes for each cDNA library (Illumina TruSeq Small RNA sample protocol). Bands corresponding to ~22 nucleotides in the original sample were size-selected (correct insert size: 146bp) after band staining and visualization under UV-light.
attributes.tag: ENA first public
attributes.value: 2016-12-01
attributes.tag: ENA last update
attributes.value: 2016-04-18
attributes.tag: External Id
attributes.value: SAMEA3932397
attributes.tag: INSDC center alias
attributes.value: Brighton and Sussex Medical School (BSMS)
attributes.tag: INSDC center name
attributes.value: Brighton and Sussex Medical School (BSMS)
attributes.tag: INSDC first public
attributes.value: 2016-12-01T17:01:24Z
attributes.tag: INSDC last update
attributes.value: 2016-04-18T17:17:48Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-4637:1072N0009
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: healthy
attributes.tag: disease staging
attributes.value: control
attributes.tag: obsolete_sex
attributes.value: male/female pool
attributes.tag: organism part
attributes.value: blood plasma
attributes.tag: sample name
attributes.value: E-MTAB-4637:1072N0009
attributes.tag: sample pool size
attributes.value: pool of 16 individuals
attributes.tag: technical duplicate of
attributes.value: 1072N0010