title: Generic sample from Homo sapiens
organism: Homo sapiens
description: HT-29 cells were cultured in RPMI1640 or McCoy's 5A medium supplemented with 10% fetal bovine serum. Cells were incubated with PBS for 72 h;the culture medium was replaced with fresh medium plus PBS 24 h after initiation of incubation.Total RNA for 5'SAGE analysis and for RT-PCR was extracted using RNA-Bee. The quality of total RNA from each cell line was analyzed using an Agilent 2100 Bioanalyzer.
attributes.tag: cell-line
attributes.value: HT-29