title: Generic sample from Pinus
organism: Pinus
description: Genomic DNA was extracted from fresh needles or seed megagametophyte tissue using the FastDNA Kit  or the DNeasy Plant Mini Kit. Chloroplast genomes were amplified in 35 separate PCR reactions. For each species, the PCR reactions were quantified, pooled into equal-molar mixtures, and converted into barcoded Illumina sequencing libraries. Individual libraries were pooled into multiplex sequencing libraries ranging from 4X-6X.
attributes.tag: barcode ACGT
attributes.value: Pinus attenuata ATTE06
attributes.tag: barcode AGCT
attributes.value: Pinus banksiana BANK03
attributes.tag: barcode CCCT
attributes.value: Pinus canariensis CANA01
attributes.tag: barcode CTGT
attributes.value: Pinus chihuahuana CHIH03
attributes.tag: barcode GGGT
attributes.value: Pinus resinosa RESI02