title: RNA-Seq polyA plus
organism: Homo sapiens
description: Total RNA was extracted from viable lymphoblastoid cell line of an anonymous male Han Chinese using Trizol (Invitrogen), treated with RNase-free DNase I to remove residual DNA. poly(A)  RNA was purified using the oligo(dT) beads. Upon chemical fragmentation, double-stranded cDNA was synthesized from these RNA samples using random hexamer-primer and reverse transcriptase (Invitrogen), and further size-selected on agarose gels.