title: Control2
organism: Caenorhabditis elegans
description: Protocols: The worms were washed off from the plates using M9 and transfer to an RNase free tube. 500 µl of Trizol were added and to 10 cycles of freezing at -80°C, thaw at 37°C and vortex 30s were done. 125 µl of BCP (1-Bromo-3-chloropropane) were added, mixed by inversion and let for incubation 3 min at RT. Samples were centrifuged at 12000 rpm for 10 min at 4°C and the clear layer was transfer to a new tube. An equal volume of 70% ethanol was added and this was transfer to a column of the RNeasy Quiogen Kit. The skn-1b(tm4241) IV mutation is a deletion mutation of the skn-1b exon. So skn-1b is not expressed A RNeasy Quiogen Kit and the manufacturer's instructions were followed. The concentration and purity of the samples were checked using the nanodrop. PolyA selection kit
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-08-13T04:04:33Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-08-16T11:42:06Z
attributes.tag: External Id
attributes.value: SAMEA5869577
attributes.tag: INSDC center name
attributes.value: University of Kent
attributes.tag: INSDC first public
attributes.value: 2020-08-13T04:04:33Z
attributes.tag: INSDC last update
attributes.value: 2019-08-16T11:42:06Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8253:Control2
attributes.tag: age
attributes.value: 1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: whole organism
attributes.tag: sample name
attributes.value: E-MTAB-8253:Control2
attributes.tag: scientific_name
attributes.value: Caenorhabditis elegans
attributes.tag: sex
attributes.value: hermaphrodite
attributes.tag: strain
attributes.value: CGCM