title: Generic sample from Pinus monticola
organism: Pinus monticola
description: Genomic DNA was extracted from fresh needles or seed megagametophyte tissue using the FastDNA Kit  or the DNeasy Plant Mini Kit. Chloroplast genomes were amplified in 35 separate PCR reactions. For each species, the PCR reactions were quantified, pooled into equal-molar mixtures, and converted into barcoded Illumina sequencing libraries. Individual libraries were pooled into multiplex sequencing libraries ranging from 4X-6X.
attributes.tag: barcode ATGT
attributes.value: Pinus monticola MONT26E1
attributes.tag: barcode CGTT
attributes.value: Pinus monticola MONT30E2
attributes.tag: barcode CCCT
attributes.value: Pinus monticola MONT36E2
attributes.tag: barcode GTAT
attributes.value: Pinus monticola MONT38E1
attributes.tag: barcode TACT
attributes.value: Pinus monticola MONT49E1