title: S7
organism: Homo sapiens
description: Protocols: PBMCs from CLL patients peripheral blood were isolated using density gradient separation (tumor load of all preparations was checked by flow cytometry and always exceeded 70% for CD19+ cells). Negative selection of CD19+ B cells from CLL patients peripheral blood was performed using the RosetteSep B-cell enrichment kit (StemCell Technologies, Vancouver, BC, Canada) following the manufacturer's instructions. The purity of all preparations was checked by flow cytometry and always exceeded 95% for CD19+ cells. Total RNA was extracted using the QIAamp RNA Blood Mini Kit (QIAGEN, Hilden, Germany) according to the manufacturer's protocol. RNA concentration was estimated usingthe Qubit Fluorometer (Invitrogen, La Jolla, CA). 1μg of total RNA was used for the library construction using the NEB Next Ultra RNA library Prep Kit (New England Biolabs, Ipswich, MA) or the TruSeq RNA Library Prep Kit v2 (Illumina, San Diego, CA). Library quantification was performed with the KAPA SYBR FAST Universal qPCR kit (KAPA Biosystems, Boston, USA).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-04-04T04:03:45Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-07-03T16:36:44Z
attributes.tag: External Id
attributes.value: SAMEA4776402
attributes.tag: IGHV mutational status
attributes.value: unmutated
attributes.tag: INSDC center name
attributes.value: Institute of Applied Biosciences / Centre for Research and Technology Hellas
attributes.tag: INSDC first public
attributes.value: 2019-04-04T04:03:45Z
attributes.tag: INSDC last update
attributes.value: 2018-07-03T16:36:44Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6962:S7
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: monocyte
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: chronic lymphocytic leukemia
attributes.tag: sample name
attributes.value: E-MTAB-6962:S7
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: stereotyped subset
attributes.value: #6
attributes.tag: tissue
attributes.value: blood