title: Sample14
organism: Mus musculus
description: Protocols: Mice were housed in laminar flow cages and permitted ad lib access to sterile food and water. Mice were exposed to aerosolized bacteria in a nose-only inhalation chamber (In-Tox products, Moriarty, NM) using a computer interface to control pressures and flows (Biaera Technologies, Frederick MD). To characterize the early course of each infection, 3-4 mice were euthanized after 4, 24 and 48hrs with intraperitoneal pentobarbital and exsanguinated by external cardiac puncture. Ten micrograms of total RNA isolated from lung tissue (equal amounts pooled from 3 individual animals) was sequentially treated with MICROBEnrich and MICROBExpress kits (Ambion) to enrich for bacterial mRNA through removal of mammalian mRNA/rRNA and bacterial rRNA, respectively. The resultant bacterial mRNA was fragmented using a RNA fragmentation kit (Ambion), which yielded fragments of 60-200 nucleotides in length. Libraries for Illumina sequencing were prepared as recommended by Illumina.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2013-03-29T17:01:40Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T16:20:54Z
attributes.tag: External Id
attributes.value: SAMEA1714533
attributes.tag: INSDC center name
attributes.value: Institute for Systems Biology
attributes.tag: INSDC first public
attributes.value: 2013-03-29T17:01:40Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T16:20:54Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-1571:Sample14
attributes.tag: age
attributes.value: 8 week
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: organism part
attributes.value: lung
attributes.tag: sample name
attributes.value: E-MTAB-1571:Sample14
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: specimen with known storage state
attributes.value: fresh specimen
attributes.tag: strain
attributes.value: BALB/c
attributes.tag: time post F. tularensis SchuS4 infection
attributes.value: 4 hour