organism: Homo sapiens
description: MCF7 cells were reverse transfected using 50nM final concentration of a pool of siRNA oligos targeting SNHG7 or a non-targeting control pool (Dharmacon) using RNAi Max at a final concentration of 3ul/ml. After 48hrs the cells were washed 2x in PBS and serum deprived in modified IMEM+10mM Hepes, 1ug/ml transferrin, 1ug/ml fibronectin, and 2mM l-glutamine for 16hrs before addition of 100ng/mL or equal volume of 10mM HCl as a vehicle control. After 8 hrs of treatment, cells were harvested , total RNA was isolated (Qiagen), multiplexed paired-end libraries were prepared (Illumina TruSeq) and sequenced on a NextSeq.
attributes.tag: isolate
attributes.value: cell line
attributes.tag: age
attributes.value: less than passage 20 from ATCC
attributes.tag: biomaterial_provider
attributes.value: ATCC
attributes.tag: sex
attributes.value: female
attributes.tag: tissue
attributes.value: mammary gland, breast; derived from metastatic site: pleural effusion
attributes.tag: cell_line
attributes.value: MCF7
attributes.tag: cell_subtype
attributes.value: ER positive
attributes.tag: cell_type
attributes.value: Epithelial
attributes.tag: dev_stage
attributes.value: breast cancer
attributes.tag: disease
attributes.value: adenocarcinoma
attributes.tag: race
attributes.value: White
attributes.tag: sample_type
attributes.value: cell culture
attributes.tag: treatment
attributes.value: siCtl_pool_72hrs_veh_8hrs
attributes.tag: biological_replicate
attributes.value: 1
attributes.tag: BioSampleModel
attributes.value: Human