title: tc1_137.1_b
organism: Mus musculus
description: Protocols: doi:10.1016/j.ymeth.2009.03.001; Shortly, liver material was crosslinked in 1% formaldehyde for 20 minutes followed by a 10 minute incubation in 500 mM glycine buffer to neutralize the formaldehyde. Samples were rinsed with PBS and frozen until use. Human material was obtained from Addenbrooke??s Hospital, Cambridge licence (08/H0308/117) and the Liver Tissue Distribution Program at the University of Pittsburgh (NIDDK Contract # N01-DK-9-2310). Formaldehyde-crosslinked/flash frozen tissue was stored at ??80C at the CRI. Tc1 mice (annotated as Tc1_with_hschr21) were generated as described in (O??Doherty et al., 2005). Mice were initially obtained from Victor Tybulewic and Elizabeth Fisher and subsequently housed and bred within the Biological Resources Unit at CRI. Tc0 mice (annotated as Tc1_without_hschr21) are Tc1 sibling mice (ie the same genetic background) that have not inherited a copy of HsChr21.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2013-03-21T17:02:36Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T16:19:31Z
attributes.tag: External Id
attributes.value: SAMEA1710307
attributes.tag: INSDC center name
attributes.value: Cancer Research UK
attributes.tag: INSDC first public
attributes.value: 2013-03-21T17:02:36Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T16:19:31Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-1104:tc1_137.1_b
attributes.tag: age
attributes.value: 2-6 month
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: Tc1_with_hschr21
attributes.tag: individual
attributes.value: tc1_137.1
attributes.tag: organism part
attributes.value: liver
attributes.tag: sample name
attributes.value: E-MTAB-1104:tc1_137.1_b
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: male