title: Generic sample from Pinus
organism: Pinus
description: Genomic DNA was extracted from fresh needles or seed megagametophyte tissue using the FastDNA Kit  or the DNeasy Plant Mini Kit. Chloroplast genomes were amplified in 35 separate PCR reactions. For each species, the PCR reactions were quantified, pooled into equal-molar mixtures, and converted into barcoded Illumina sequencing libraries. Individual libraries were pooled into multiplex sequencing libraries ranging from 4X-6X.
attributes.tag: barcode AGCT
attributes.value: Pinus albicaulis ALBI05
attributes.tag: barcode ACGT
attributes.value: Pinus aristata ARIS02
attributes.tag: barcode CCCT
attributes.value: Pinus ayacahuite AYAC01
attributes.tag: barcode CTGT
attributes.value: Pinus cembra CEMB03
attributes.tag: barcode GGGT
attributes.value: Pinus flexilis FLEX13
attributes.tag: barcode TACT
attributes.value: Pinus peuce PEUC03