title: 4OHT_Control_48h_RNASeq_Rep2
organism: Mus musculus
attributes.tag: source_name
attributes.value: Embryonic Stem Cells
attributes.tag: parental esc line
attributes.value: E14
attributes.tag: zbtb11 locus genotype
attributes.value: Zbtb11WT/WT
attributes.tag: rosa26 locus genotype
attributes.value: WT
attributes.tag: treatment
attributes.value: 4-hydorxytamoxifen (250nM)
attributes.tag: treatment protocol
attributes.value: To induce Zbtb11 KO, growth media was supplemented with 4-hydroxytamoxifen (4OHT) pre-slubilised in ethanol. 4OHT was added to a final concentration of 250nM from a 1000x stock solution. As control, Zbtb11lox/lox Rosa26ERt2-Cre were treated with the carrier ethanol, or Zbtb11WT/WT Rosa26WT were treated with 4OHT.
attributes.tag: antibody
attributes.value: N/A