title: Sample 4
organism: Homo sapiens
description: Protocols: At 2 h (treated and controls), 6 h and 24 h after treatment, cells were harvested by trypsination and washed in PBS. Cell suspensions were divided into aliquots for whole-cell analysis, subcellular fractionation and RNA sequencing. A431 human cells (DSMZ #ACC-91, tested for, and found free of, Mycoplasma contamination) were cultured in Dulbecco's Modified Eagle Medium supplemented with 10% fetal bovine serum, 1% penicillin/streptomycin, non-essential amino acids, and sodium pyruvate, all from Invitrogen. Twenty four hours after seeding, the A431 cell cultures (in triplicates) were treated with gefitinib to induce altered gene/protein expression. Controls were left untreated. Total RNA was prepared from A431 cells (aliquots from the samples run on mass spectrometry) in biological triplicates using RNeasy Mini kit (Qiagen). cDNA libraries were constructed according to a modified Illumina RNA-seq library preparation protocol described in http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0021910
attributes.tag: ENA first public
attributes.value: 2016-12-01
attributes.tag: ENA last update
attributes.value: 2016-11-30
attributes.tag: External Id
attributes.value: SAMEA17820418
attributes.tag: INSDC center alias
attributes.value: Science for Life Laboratory, Department of Biochemistry and Biophysics, Stockholm University
attributes.tag: INSDC center name
attributes.value: Science for Life Laboratory, Department of Biochemistry and Biophysics, Stockholm University
attributes.tag: INSDC first public
attributes.value: 2016-12-01T17:01:25Z
attributes.tag: INSDC last update
attributes.value: 2016-11-30T10:06:59Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-5285:Sample 4
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: A431
attributes.tag: cell type
attributes.value: epithelial cell
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: squamous cell carcinoma
attributes.tag: organism part
attributes.value: skin
attributes.tag: sample name
attributes.value: E-MTAB-5285:Sample 4