title: DNA derived from blood of a normal patient of Caucasian descent.
organism: Homo sapiens
description: 100 ml of whole blood was drawn from a healthy, anonymous volunteer of Caucasian descent, who is properly consented, at the Stanford University Hospital. Peripheral blood mononuclear cells (PBMCs) were isolated with Ficoll gradient (Lymphocyte Separation Medium, MP Biomedicals, OH) centrifugation according to the manufacture’s protocol. Genomic DNA was prepared from isolated PBMCs with the AllPrep DNA/RNA/Protein Mini Kit (QIAGEN) and treated with RNase A to remove remaining RNA. DNA concentration was quantified with Invitrogen’s Qubit Fluorometer. This DNA was then used for exome enrichment and sequencing downstream.