title: Pinus chloroplast genomes
organism: Pinus
description: Genomic DNA was extracted from fresh needles or seed megagametophyte tissue using the FastDNA Kit  or the DNeasy Plant Mini Kit. Chloroplast genomes were amplified in 35 separate PCR reactions. For each species, the PCR reactions were quantified, pooled into equal-molar mixtures, and converted into barcoded Illumina sequencing libraries. Individual libraries were pooled into multiplex sequencing libraries ranging from 4X-6X.
attributes.tag: barcode AGCT
attributes.value: Pinus armandii ARMA04
attributes.tag: barcode CCCT
attributes.value: Pinus parviflora var. pentaphylla PARV14
attributes.tag: barcode CTGT
attributes.value: Pinus sibirica SIBI02
attributes.tag: barcode TACT
attributes.value: Pinus squamata SQUA02
attributes.tag: barcode GGGT
attributes.value: Pinus strobus STRO27
attributes.tag: barcode ACGT
attributes.value: Pinus wallichiana WALL02