title: COPD8
organism: Homo sapiens
description: Protocols: Left-over frozen lung tissue from 10 ex-smoker stage IV COPD patients and 8 ex-smoker non-COPD controls was used. All subjects were Caucasian. All samples were obtained from peripheral lung tissue according to national and local ethical guidelines and the research code of the University Medical Centre Groningen. Non-COPD control (referred throughout as 'control') was defined with lung function FEV1/FVC ≥ 70% and FEV1 >90% predicted. Subjects in this category did not have a history of lung disease, apart from lung cancer for which the patients underwent surgery. For these cases, lung tissue samples were taken as distant from the tumor as possible. Thus, any possible effect of the tumor on the lung tissue was minimised. All COPD patients suffered from severe emphysema and underwent lung transplantation. Total RNA from parenchymal lung samples was isolated using TRIzol Reagent (Ambion, Foster City, CA), according to manufacturer's instructions. We extracted PolyA-tailed mRNA fraction from total RNA and prepared RNA-seq libraries using NEXTFlex Rapid Directional qRNA-Seq Kit (Bioo Scientific, Austin, TX) according to the manufacturer's protocol.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-08-13T04:04:33Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-08-16T11:13:17Z
attributes.tag: External Id
attributes.value: SAMEA5869566
attributes.tag: INSDC center name
attributes.value: European Research Institute for the Biology of Ageing
attributes.tag: INSDC first public
attributes.value: 2020-08-13T04:04:33Z
attributes.tag: INSDC last update
attributes.value: 2019-08-16T11:13:17Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8251:COPD8
attributes.tag: age
attributes.value: 52
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: clinical information
attributes.value: ex-smoker
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: chronic obstructive pulmonary disease
attributes.tag: disease staging
attributes.value: stage IV
attributes.tag: ethnic group
attributes.value: European
attributes.tag: individual
attributes.value: COPD 8
attributes.tag: organism part
attributes.value: lung
attributes.tag: sample name
attributes.value: E-MTAB-8251:COPD8
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: male