title: Micronucleated Daughter Pair 5.3
organism: Homo sapiens
description: MN daughter cell after Cas9 treatment targeted chr5q
attributes.tag: isolate
attributes.value: cell line
attributes.tag: age
attributes.value: not applicable
attributes.tag: biomaterial_provider
attributes.value: ATCC
attributes.tag: sex
attributes.value: female
attributes.tag: tissue
attributes.value: Retina, eye; pigmented epithelium
attributes.tag: cell_line
attributes.value: hTERT RPE-1
attributes.tag: cell_subtype
attributes.value: RPE-1 rtTA-T2A-neo; dox(Cas9); sg Chr5q (puro); eGFP-H2B; tdRFP-NLS
attributes.tag: cell_type
attributes.value: Epithelial cells immortalized with hTERT
attributes.tag: culture_collection
attributes.value: ATCC:CRL-4000
attributes.tag: phenotype
attributes.value: micronucleus
attributes.tag: sample_type
attributes.value: single cell
attributes.tag: treatment
attributes.value: p53 siRNA, serum starve synchronization, doxycycline induction of Cas9
attributes.tag: date_collected
attributes.value: 5/16/17
attributes.tag: plate_location
attributes.value: J
attributes.tag: acquisition_method
attributes.value: FACS
attributes.tag: CAS9_type
attributes.value: Inducible
attributes.tag: target_chromosome
attributes.value: Chr5q
attributes.tag: BioSampleModel
attributes.value: Human