title: E18.5 pancreas islets
organism: Mus musculus
description: Protocols: Isolated islets were GFP sorted using mouse lines that expressed GFP with a minimal Pdx1 promoter. No MARIS sorting. After sorting, cells were pelleted by centrifugation at 3000 g for 5min at 4C. The supernatant was discarded. Total RNA was isolated from the pellet using the RecoverAll Total Nucleic Acid Isolation kit (Ambion), starting at the protease digestion stage of manufacturer-recommended protocol. The following modification to the isolation procedure was made: instead of incubating cells in digestion buffer for 15 minutes at 50C and 15 minutes at 80C, we carried out the incubation for 3 hours at 50C. Cell lysates were frozen at -80C overnight before continuing the RNA isolation by the manufacturer's instructions. NA
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2015-03-17T16:40:38Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T23:43:19Z
attributes.tag: External Id
attributes.value: SAMEA3308494
attributes.tag: INSDC center name
attributes.value: Massachusetts Institute of Technology
attributes.tag: INSDC first public
attributes.value: 2015-03-17T16:40:38Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T23:43:19Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-3417:E18.5 pancreas islets
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: E18.5
attributes.tag: organism part
attributes.value: islet
attributes.tag: sample name
attributes.value: E-MTAB-3417:E18.5 pancreas islets
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: strain or line
attributes.value: Pdx1 minimal promoter-GFP on mixed/outbred background