title: RNA_siEXOC5_1
organism: Homo sapiens
attributes.tag: sample_name
attributes.value: DRS012726
attributes.tag: sample comment
attributes.value: This sample is total RNA in the EXOC5-depleted HeLa cells.Approximately 1μg RNA was used to sequence an RNA-seq library, using mRNA-seq Sample Preparation Kit (Illumina) according to manufacturer's protocol.HeLa cells were grown in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% Fetal Bovine Serum and antibiotics at 37oC at 5% CO2 in a humidified incubator.siRNAs were transfected using Lipofectamine RNAiMAX (Invitrogen), according to the instructions from the manufacturer. Following the siRNA duplex transfection (final concentration 10nM), cells were harvested for 72h after the transfection.