title: Nipponbare STARR-seq biological replicate 1
organism: Oryza sativa Japonica Group
description: Enhancer by STARR seq from Oryza sativa Japonica
attributes.tag: cultivar
attributes.value: Nipponbare
attributes.tag: age
attributes.value: 2 weeks
attributes.tag: sex
attributes.value: neuter
attributes.tag: tissue
attributes.value: Seedling
attributes.tag: growth_protocol
attributes.value: The rice cultivar Nipponbare and Zhonghua11 (Oryza sativa L. ssp Japonica) seeds were placed on filter papers moistened with water in Petri dishes at room temperature to promote germination(3days) and were then transferred to culture box at 37 degree centigrade for about 24h. and then transplant into the box with soil. The rice plants were grown in a growth room at degree centigrade with a 12/12 h light/dark pthotoperiod and illuminated by a bank of 400 W metal halide lamps, a light internsity at plant height of 400 umol m-2 s-1 and a relative humidity of approximately 60%. Two-week-old rice seedlings were collected for the experiments.
attributes.tag: treatment
attributes.value: We isolated the protoplasts following a published protocol with minor modifications. Green tissues from the stem of 80-120 rice seedlings were used.
attributes.tag: BioSampleModel
attributes.value: Model organism or animal