title: 20151027_LUHMGU5_e36
organism: Homo sapiens
description: Protocols: 3 µg of RNA was treated with TURBO DNase, followed by RNeasy Minelute RNA cleanup kit and RNA quaility was confirmed using Agilent 2100 Bioanalyzer with RNA Pico 6000 kit (RIN values >8). 0.5 µg of RNA was used for Lexogen QuantSeq-REV libraries. cDNA libraries were prepared according to Lexogen QuantSeq Rev method manufacturer's protocol, using a poly(T) primer for reverse transcription. The library was sequenced using Illumina HiSeq, producing 60 nt single-end reads and 10 nt index reads H9 human ESCs (H9 line) were cultured in mTeSR1 medium on Matrigel-coated plates. Colonies were passaged for maintanence by Gentle Cell Dissociation Reagent We used shRNA targeting human TDP-43 (GAAACACAAGTGAAAGTAA) or a control targeting firefly luciferase (CGTACGCGGAATACTTCGA) driven by the H1 promoter in the vector FUW coexpressing TagRFP both for transfection and transduction, while maintaining hESC in pluripotency or differentiation growth conditions. Lexogen 3'-end QuandSeq Reverse
attributes.tag: ENA first public
attributes.value: 2019-02-25
attributes.tag: ENA last update
attributes.value: 2016-09-20
attributes.tag: External Id
attributes.value: SAMEA4444117
attributes.tag: INSDC center alias
attributes.value: Institute of Molecular Life Sciences and Swiss Institute of Bioinformatics
attributes.tag: INSDC center name
attributes.value: Institute of Molecular Life Sciences and Swiss Institute of Bioinformatics
attributes.tag: INSDC first public
attributes.value: 2019-02-25T17:03:10Z
attributes.tag: INSDC last update
attributes.value: 2016-09-20T11:40:38Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-5090:20151027_LUHMGU5_e36
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: undifferentiated human embryonic stem cell
attributes.tag: common name
attributes.value: human
attributes.tag: replicate
attributes.value: 5
attributes.tag: rna interference
attributes.value: shRNA transduction targeting TDP-43
attributes.tag: sample name
attributes.value: E-MTAB-5090:20151027_LUHMGU5_e36