title: Sample 19
organism: Homo sapiens
description: Protocols: Blood-derived lymphoblastoid cell lines for 36 individuals with autism spectrum disorder were obtained from the Simons Simplex Collection. For two individuals the age of diagnosis is missing due to lack of consent for distributing this information. Cells were cultured in Culture Medium (RPMI 1640, 2mM L-glutamine, 15% fetal bovine serum) to a maximum cell density of 1 × 106 cells/ml. When cells reached maximum density, they were passaged by dissociating the cells by pipetting up and down 4 or 5 times and seeding to a density of 200,000-500,000 viable cells/ml. Cells were grown under 37°C, 5% CO2 conditions for 10 days. Approximately 5 × 105 cells were then detached and spun down at 300 × g for 5 minutes at 4°C. RNA was extracted using RNeasy® Plus Micro Kit (QIAGEN) following manufacturer's protocol. RNA quality was assessed using Agilent RNA 6000 Nano Kit (Agilent Technologies) and ran on Bioanalyzer 2100 (Agilent Technologies). RNA-seq libraries were generated by TruSeq® Stranded Total RNA Library Prep Kit with Ribo-Zero Gold Set A (Illumina).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-11-25T17:03:25Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-11-15T19:20:35Z
attributes.tag: External Id
attributes.value: SAMEA5091993
attributes.tag: INSDC center name
attributes.value: Department of Psychiatry, UCSF Weill Institute for Neurosciences
attributes.tag: INSDC first public
attributes.value: 2018-11-25T17:03:25Z
attributes.tag: INSDC last update
attributes.value: 2018-11-15T19:20:35Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7351:Sample 19
attributes.tag: age
attributes.value: 9.8
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: lymphoblast
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: juvenile stage
attributes.tag: disease
attributes.value: autism spectrum disorder
attributes.tag: individual
attributes.value: 12853
attributes.tag: organism part
attributes.value: blood
attributes.tag: sample name
attributes.value: E-MTAB-7351:Sample 19
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: male