title: BIOPSY_3691
organism: Homo sapiens
description: Protocols: Tissue for ChIP-seq analyses was from patients who were treated at the Netherlands Cancer Institute.Three biopsies (14-gauge, 15 mm thick) were taken from each tumour, two of which where frozen with liquid nitrogen and one was formalin-fixed for direct clinical assessment. Tumour cell percentage was determined using Hematoxylin and Eosin (H&E), and tissue was only used for ChIP-seq analysis when tumour cell percentage was higher then 50%. All samples stained positive for Estrogen Receptor. Samples were processed and chromatin was extracted as described (Schmidt et al., Methods 48:3 pp240-248 2009) but with a number of modifications. During the immunoprecipitation, 100mg/ml glycogen (Invitrogen) or 20mg/ml recombinant histone 2B (M2505S; New England Biolabs) and 1mg/ml human mRNA (Invitrogen) was added as carriers for the ChIP. During the entire procedure, non-sticky eppendorf tubes were used (13-698-794; Fisher Scientific).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2013-04-01T17:02:48Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T16:18:13Z
attributes.tag: External Id
attributes.value: SAMEA1713770
attributes.tag: INSDC center name
attributes.value: Cancer Research UK Cambridge Institute
attributes.tag: INSDC first public
attributes.value: 2013-04-01T17:02:48Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T16:18:13Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-1534:BIOPSY_3691
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: epithelial
attributes.tag: common name
attributes.value: human
attributes.tag: disease state
attributes.value: breast cancer
attributes.tag: organism part
attributes.value: breast
attributes.tag: sample name
attributes.value: E-MTAB-1534:BIOPSY_3691
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: female