title: BM18
organism: Homo sapiens
description: Protocols: All research involving human blood and tissue samples was approved by the local Research Ethics Committee. Written informed consent was obtained from each participant in accordance with the Declaration of Helsinki. Peripheral blood mononuclear cells were isolated from healthy donors via density gradient centrifugation using Lymphoprep (Axis- Shield). Human split skin samples (0.4 mm) were excised from healthy donors undergoing elective surgery using a dermatome. Sections were cut into 1 cm2 fragments and digested in a mixture of dispase II (1.25 U/ml), collagenase D (1 mg/ml), and DNase I (20 U/ml) (all from Roche) for 15-30 min at 37°C. The epidermis was separated from the dermis using forceps, and both the epidermis and dermis were subsequently cultured for 48 hr at 37°C in AB-RPMI. Single-cell suspensions of migrated cells were obtained from the culture medium by passing through a 40-μm pore mesh, washed, and resuspended in AB-RPMI. Total RNA was isolated using a Qiagen RNeasy Micro Kit (Qiagen), and cDNA was generated using a SMARTer Kit (ClonTech). Libraries were prepared using a Nextera Kit (Illumina). Next-generation sequencing was performed using the Illumina HiSeq 2500 System with TruSeq Technology (Illumina).
attributes.tag: ENA first public
attributes.value: 2017-12-21
attributes.tag: ENA last update
attributes.value: 2017-12-21
attributes.tag: External Id
attributes.value: SAMEA104447896
attributes.tag: INSDC center alias
attributes.value: Cardiff University
attributes.tag: INSDC center name
attributes.value: Cardiff University
attributes.tag: INSDC first public
attributes.value: 2017-12-21T17:03:29Z
attributes.tag: INSDC last update
attributes.value: 2017-12-21T14:13:00Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6370:BM18
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: CD8-Positive T-Lymphocytes
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: normal
attributes.tag: organism part
attributes.value: zone of skin
attributes.tag: phenotype
attributes.value: CCR8 negative
attributes.tag: sample name
attributes.value: E-MTAB-6370:BM18