title: Full-scale activated sludge biomass
organism: activated sludge metagenome
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-11-20T17:04:04Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-11-20T11:08:32Z
attributes.tag: External Id
attributes.value: SAMEA5096271
attributes.tag: INSDC center name
attributes.value: AAU-BIO
attributes.tag: INSDC first public
attributes.value: 2018-11-20T17:04:04Z
attributes.tag: INSDC last update
attributes.value: 2018-11-20T11:08:32Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: core_RAN_2009_8_69
attributes.tag: chimera check
attributes.value: ChimeraSlayer
attributes.tag: collection date
attributes.value: 2009-08-01
attributes.tag: common name
attributes.value: activated sludge metagenome
attributes.tag: environment (biome)
attributes.value: freshwater biome [ENVO:00000873]
attributes.tag: environment (feature)
attributes.value: wastewater treatment plant [ENVO:00002043]
attributes.tag: environment (material)
attributes.value: activated sludge [ENVO:00002046]
attributes.tag: geographic location (country and/or sea)
attributes.value: Denmark
attributes.tag: geographic location (latitude)
attributes.value: 0
attributes.tag: geographic location (longitude)
attributes.value: 0
attributes.tag: investigation type
attributes.value: mimarks-survey
attributes.tag: nucleic acid extraction
attributes.value: http://www.mpbio.com/US/Pages/Product.aspx?pid=116560200
attributes.tag: pcr conditions
attributes.value: initial denaturation: 95degC_5min; annealing: 50degC_0.5min; elongation: 72degC_1min; final elongation: 72degC_7min; total cycles: 20. Adaptor annealling: denaturation: 95degC_5min; annealing: 50degC_0.5min; elongation: 72degC_1min; final elongation: 72degC_7min; total cycles: 7
attributes.tag: pcr primers
attributes.value: FWD:GTGCCAGCMGCCGCGGTAA REV:GGACTACHVGGGTWTCTAAT
attributes.tag: project name
attributes.value: DK-sludge-core
attributes.tag: reactor type
attributes.value: enhanced biological phosphorus removal
attributes.tag: sample name
attributes.value: core_RAN_2009_8_69
attributes.tag: scientific_name
attributes.value: activated sludge metagenome
attributes.tag: sequencing method
attributes.value: Illumina
attributes.tag: target gene
attributes.value: 16S rRNA
attributes.tag: target subfragment
attributes.value: V4
attributes.tag: wastewater/sludge environmental package
attributes.value: wastewater/sludge