title: H3K4me1 ChIP
organism: Homo sapiens
description: Protocols: ChIP for H3K4me1 (Abcam ab8895) was performed using sonicated extracts from formaldehyde-fixed nuclei. HeLa S3 cells were grown in DMEM + 10% foetal calf serum (FCS) Untreated DNA was extracted from immunoprecipitated or input chromatin using Qiagen MinElute kit and re-sonicated to obtain a mean fragment size of around 300bp. Resonicated DNA was ligated to NEBNext adapters using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs), and amplified by PCR (8 cycles).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-04-04T04:03:51Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-02-01T16:06:21Z
attributes.tag: External Id
attributes.value: SAMEA5315522
attributes.tag: INSDC center name
attributes.value: Universite Cote d'Azur, Inserm, CNRS
attributes.tag: INSDC first public
attributes.value: 2019-04-04T04:03:51Z
attributes.tag: INSDC last update
attributes.value: 2019-02-01T16:06:21Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7643:H3K4me1 ChIP
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: HeLa-S3
attributes.tag: common name
attributes.value: human
attributes.tag: sample name
attributes.value: E-MTAB-7643:H3K4me1 ChIP
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: female