title: Sample 1
organism: Mus musculus
description: Protocols: Stable GM6377 OR LOC108167440- and EGFP-expressing SP 2/0 cells, and the control EGFP-expressing SP 2/0 cells were collected after after cultured for 2 days in fresh medium. Samples were washed twice with PBS and 1 mL of TRIzol per 1 x 107 cell pellets was added and frozen in -80℃. GM6377 OR LOC108167440 cDNA (General Biosystems, Anhui, China) was cloned into lentiviral vector EGFP-expressing LV201 (Fugene Corp., Guangzhou, China) to generate GM6377 OR LOC108167440 protein. Empty LV201 and GM6377 OR LOC108167440-expressing LV201 was transfected into SP 2/0 cells (ATCC® CRL-1581™, https://www.atcc.org/products/all/CRL-1581.aspx), and stable transfectants were identified by EGFP expression and drug selection (Puromycin, Sigma, 10 μg/ml). Stable GM6377 OR LOC108167440-expressing SP 2/0 cells and LV201-transduced SP 2/0 cells were cultured in fresh ATCC-formulated Dulbecco's Modified Eagle's Medium (Catalog No. 30-2002) containing 10% FBS, 2 mM glutamine, penicillin (100 IU/ml), streptomycin (100 g/ml), and 50 mM 2-mercaptoethanol. Cells were collected on ice after cultured for 2 days in fresh medium. Data were aligned using STAR with options --outFilterScoreMinOverLread 0.4 --outFilterMatchNminOverLread 0.4, against the C3H/HeJ genome as a reference (ftp://ftp.ensembl.org/pub/release-89/fasta/mus_musculus_c3hhej/dna/Mus_musculus_c3hhej.C3H_HeJ_v1.dna_sm.toplevel.fa.gz). Total RNA from cells was isolated and purified using the RNeasy Mini Kit (Qiagen, Venlo, Netherlands). All RNA content in the samples was measured with a NanoDrop®ND-1000 spectrophotometer, and the RNA quality was assessed using RNA 6000 NanoChips with the Agilent 2100 Bioanalyzer (Agilent, Palo Alto, CA, USA). Libraries were prepared using the TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold (Illumina).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-08-13T04:04:16Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-08-13T13:06:13Z
attributes.tag: External Id
attributes.value: SAMEA4827976
attributes.tag: INSDC center name
attributes.value: Beijing Institute of Basic Medical Sciences
attributes.tag: INSDC first public
attributes.value: 2020-08-13T04:04:16Z
attributes.tag: INSDC last update
attributes.value: 2018-08-13T13:06:13Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7139:Sample 1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: Sp2/0-Ag14
attributes.tag: cell type
attributes.value: lymphocyte
attributes.tag: common name
attributes.value: house mouse
attributes.tag: disease
attributes.value: multiple myeloma
attributes.tag: genotype
attributes.value: empty vector LV201
attributes.tag: organism part
attributes.value: mixed spleen and skin
attributes.tag: phenotype
attributes.value: empty vector
attributes.tag: sample name
attributes.value: E-MTAB-7139:Sample 1
attributes.tag: scientific_name
attributes.value: Mus musculus