title: PLKO_2
organism: Homo sapiens
description: Protocols: Control vs CFTR knockdown, and vehicle vs CFTRinh-172 (10µM) treated endothelial cells were collected. RNA was extracted using the TRIzol kit following the manufacturer's instructions. Quality and quantity were measured on a Nanodrop. Sequence libraries were prepared with the Lexogen QuantSeq 3' mRNA-Seq Library prep kit according to the manufacturer's protocol. Samples were indexed to allow for multiplexing. Library quality and size range was assessed using a Bioanalyzer (Agilent Technologies) with the DNA 1000 kit (Agilent Technologies) according to the manufacturer recommendations.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-07-14T04:04:14Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-12-13T16:50:49Z
attributes.tag: External Id
attributes.value: SAMEA6415529
attributes.tag: INSDC center name
attributes.value: Laboratory of Angiogenesis & Vascular Metabolism Vesalius Research Centre (VRC) VIB Center for Cancer Biology (CCB)
attributes.tag: INSDC first public
attributes.value: 2020-07-14T04:04:14Z
attributes.tag: INSDC last update
attributes.value: 2019-12-13T16:50:49Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8599:PLKO_2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: HUVEC cell line
attributes.tag: cell type
attributes.value: endothelial cell
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: normal
attributes.tag: organism part
attributes.value: umbilical vein
attributes.tag: passage number
attributes.value: 3
attributes.tag: phenotype
attributes.value: wild type
attributes.tag: rna interference
attributes.value: scrambled shRNA
attributes.tag: sample name
attributes.value: E-MTAB-8599:PLKO_2
attributes.tag: scientific_name
attributes.value: Homo sapiens