title: Sm
organism: Zindervirus SP6
description: SP6* was grown using serial transfer method at 37*C on E. coli K-12 host in LB broth with the addition of 2ug/mL N-methyl-N'nitro nitrosoguanidine(mutagen)added to media every fourth transfer. The final evolved population was then used to isolate DNA for sequencing.