title: Sample 2
organism: Homo sapiens
description: Protocols: >1000-fold coverage of mutagenized cells (libraries A and B) was infected with either RV-C15 (MOI=1 PFU/cell) or EV-D68 Missouri (MOI=1 PFU/cell). RV-C15 infection was repeated for an additional round at 6 days post-infection. As soon as appearance of visibly viable colonies was observed, populations of virus-resistant cells were pooled and harvested. Uninfected starting populations of mutagenized cells were used as the unselected reference. Total genomic DNA from both virus-resistant and uninfected cells was respectively extracted using QIAamp DNA Mini Kit (Qiagen). The inserted guide RNA sequences were retrieved from the genomic DNA by PCR amplification.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-07-10T04:04:21Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-07-08T16:07:34Z
attributes.tag: External Id
attributes.value: SAMEA5762537
attributes.tag: INSDC center name
attributes.value: Department of Microbiology & Immunology, Stanford University School of Medicine
attributes.tag: INSDC first public
attributes.value: 2019-07-10T04:04:21Z
attributes.tag: INSDC last update
attributes.value: 2019-07-08T16:07:34Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8125:Sample 2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: CDHR3 and Cas9 expressing H1-HeLa cells
attributes.tag: common name
attributes.value: human
attributes.tag: genetic modification
attributes.value: CRISPR knockout by GeCKO v2 library B (PMID: 25075903)
attributes.tag: infect
attributes.value: uninfected/unselected control
attributes.tag: sample name
attributes.value: E-MTAB-8125:Sample 2
attributes.tag: scientific_name
attributes.value: Homo sapiens