title: ATAC-Seq using dendritic cells of control and TTT/AAA beta2 integrin knock-in mice
organism: Mus musculus
description: DCs were generated by culturing bone marrow for 9-10 days in 10 ng/ml GM-CSF (Peprotech) RPMI +10% FCS, 100 U/ml penicillin-streptomycin and 2 mM L-glutamine, with media addition/change on days 3, 6, and 8. ATAC-Seq was performed according to the omni-ATAC protocol and using 50000 cells per sample. Paired-end (2x42 bp) sequencing was performed on Illumina NextSeq 500.
attributes.tag: strain
attributes.value: C57BL/6NCrl
attributes.tag: age
attributes.value: 8-39 weeks
attributes.tag: sex
attributes.value: pooled male and female
attributes.tag: tissue
attributes.value: Bone marrow derived dendritic cells
attributes.tag: BioSampleModel
attributes.value: Model organism or animal