title: The effect of MNase on nucleosome positioning data
organism: Saccharomyces cerevisiae
description: Micrococcal nuclease (MNase) is frequently used to determine nucleosome positions both in vitro and in vivo. This is achieved by digesting chromatin by MNase and selecting nucleosomal sized DNA fragments (~150 base pairs). To check to what extend the resulting data is biased by the use MNase and the size selection, a control experiment was performed, where naked yeast DNA was digested with MNase and fragments of 150 base pairs were selected. The MNase data shows a good correspondence to the data obtained by digesting chromatin assembled in vitro as well as chromatin isolated in vivo.