title: PolyA Selected U2AF1 b siRNA Depletion Replicate 1
organism: Homo sapiens
description: Protocols: Cell lines were grown under standard conditions in DMEM supplemented with 10% (v/v) bovine calf serum (Gibco BRL) Transfections of small interfering RNAs (siRNAs) were carried out in 6- or 12- well plates using jetPRIME (Polyplus) according to manufacturer's recommendations. The cells received treatment with siRNA twice - 24h post seeding, and 48h post seeding when splitting into a new plate. PolyA library selection was performed using NEBNext poly(A) mRNA magnetic isolation module (E7490L) according to the manufacturer's specifications.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2015-03-16T17:04:49Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T18:27:57Z
attributes.tag: External Id
attributes.value: SAMEA2624024
attributes.tag: INSDC center name
attributes.value: University of Southampton
attributes.tag: INSDC first public
attributes.value: 2015-03-16T17:04:49Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T18:27:57Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-2682:PolyA Selected U2AF1 b siRNA Depletion Replicate 1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: HEK293T
attributes.tag: common name
attributes.value: human
attributes.tag: library selection
attributes.value: PolyA+
attributes.tag: sample name
attributes.value: E-MTAB-2682:PolyA Selected U2AF1 b siRNA Depletion Replicate 1
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: female