title: H1
organism: Homo sapiens
attributes.tag: source_name
attributes.value: human embryonic stem cells, WiCell
attributes.tag: cell line
attributes.value: H1
attributes.tag: lentiviral transgenes
attributes.value: no
attributes.tag: passage number
attributes.value: passage 40
attributes.tag: cell type
attributes.value: embronic stem cells
attributes.tag: culture protocols
attributes.value: Human ESCs (H1 and H9, WiCell, Wisconsin) and iPSCs were maintained in E8 medium [27] on MatrigelÂ­coated tissue culture plates. E8 medium contained DMEM/F12, 64 mg/L LÂ­ascorbic acid 2Â­phosphate sesquimagnesium, 13.6 Âµg /L sodium selenium, 1.7 g/L NaHCO3, 1 g/L sodium chloride, 10 ng/ml FGF2, 20 Âµg/ml insulin, 10 Âµg/ml transferrin and 2 Âµg/L TGFÎ²1
attributes.tag: harvest time
attributes.value: 80% confluence