title: Generic sample from Pinus monticola
organism: Pinus monticola
description: Genomic DNA was extracted from fresh needles or seed megagametophyte tissue using the FastDNA Kit  or the DNeasy Plant Mini Kit. Chloroplast genomes were amplified in 35 separate PCR reactions. For each species, the PCR reactions were quantified, pooled into equal-molar mixtures, and converted into barcoded Illumina sequencing libraries. Individual libraries were pooled into multiplex sequencing libraries ranging from 4X-6X.
attributes.tag: barcode AACT
attributes.value: Pinus monticola MONT06E1
attributes.tag: barcode ATGT
attributes.value: Pinus monticola MONT07E2
attributes.tag: barcode CACT
attributes.value: Pinus monticola MONT08E1
attributes.tag: barcode GTAT
attributes.value: Pinus monticola MONT12E1
attributes.tag: barcode GGGT
attributes.value: Pinus monticola MONT14E1