title: Generic sample from Mus musculus
organism: Mus musculus
description: We obtained one female mouse each from the C57BL/6J (B6) and DBA/2J (DBA) inbred strains (Jackson Laboratory, Bar Harbor, ME). The B6 individual was obtained in January, 2006. This mouse was a retired foundation stock breeder (F226), and is thus derived from the colony nucleus and should be minimally diverged from the reference genome (Waterston et al. 2002). The DBA mouse was obtained in December, 2004. This mouse was ordered through the standard mechanism and is not pedigreed. These are precisely the same samples analyzed in our previous aCGH study (Egan et al. 2007).   DNA preparation. DNA was isolated using the Puregene Kit (Gentra Systems), with the following modifications. Livers were flash frozen in liquid nitrogen, ground with mortar and pestle, and dounce-homogenized in 20 ml cell lysis solution. After isolation of crude DNA from liver and tail, further extractions were performed with phenol/chloroform/isoamyl alcohol, and chloroform/isoamyl alcohol, and DNA was precipitated with 2 volumes isopropanol and 1/10 volume sodium acetate.