title: MCF-7-2
organism: Homo sapiens
description: Protocols: MCF7 cells were cultured in DMEM supplemented with 10% fetal bovine serum (FBS) and 1% antibiotics. For hormone depletion, cells were grown in phenol red-free DMEM with 5% charcoal-treated FBS and 1% antibiotics for 3 days. Hormone-deprived cells were treated for 3 hours with 10nM Estradiol, dissolved in 0.01% DMSO Cells were fixed with 1% PFA for 10 minutes and processed as described (Schmidt et al., Methods 48:3 pp240-248 2009). For each ChIP, 10ug of antibody was used, and 100ul of Protein A magnetic beads (Invitrogen). The antibody used was raised to detect RIP140 (6D7). ChIP was performed and libaries were contructed as described (Schmidt et al., Methods 48:3 pp240-248 2009).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2015-03-16T17:04:47Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T17:40:19Z
attributes.tag: External Id
attributes.value: SAMEA2532530
attributes.tag: INSDC center name
attributes.value: CR-UK Cambridge Institute
attributes.tag: INSDC first public
attributes.value: 2015-03-16T17:04:47Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T17:40:19Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-2576:MCF-7-2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: MCF-7
attributes.tag: cell type
attributes.value: epithelial
attributes.tag: common name
attributes.value: human
attributes.tag: sample name
attributes.value: E-MTAB-2576:MCF-7-2
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: female
attributes.tag: specimen with known storage state
attributes.value: frozen specimen
attributes.tag: transcription factor
attributes.value: RIP140