title: TM10_P
organism: Homo sapiens
description: Protocols: CD14+ monocytes were isolated from peripheral blood mononuclear cells (PBMC) using magnetic positive selection (Miltenyi Biotec). Monocyte culture lysates were collected in RLT buffer (Qiagen) and total RNA purified using the RNeasy Mini kit (Qiagen). RNA was subjected to DNase treatment to remove contaminating genomic DNA using a TURBO DNA-free kit (Ambion, Life Technologies). Concentration and quality of all RNA samples were assessed using the Nanodrop Spectrophotometer and Agilent Bioanalyzer, respectively. The KAPA mRNA HyperPrep Kit (Roche Diagnostics) was used to construct stranded mRNA-Seq libraries from up to 500 ng intact total RNA.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-07-14T04:04:08Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-07-31T11:13:53Z
attributes.tag: External Id
attributes.value: SAMEA5819818
attributes.tag: INSDC center name
attributes.value: UNIVERSITY COLLEGE LONDON
attributes.tag: INSDC first public
attributes.value: 2020-07-14T04:04:08Z
attributes.tag: INSDC last update
attributes.value: 2019-07-31T11:13:53Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8162:TM10_P
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: monocytes
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: tuberculosis
attributes.tag: disease staging
attributes.value: latent infection
attributes.tag: immunophenotype
attributes.value: CD14+
attributes.tag: organism part
attributes.value: blood
attributes.tag: sample name
attributes.value: E-MTAB-8162:TM10_P
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: stimulus
attributes.value: Mycobacterium tuberculosis purified protein derivative (PPD)