title: Methylobacterium_sp. 22A_RNAseq_CaHo
organism: Methylobacterium sp. MA-22A
description: Methylobacterium sp. 22A was grown in 100-ml methanol medium prepared in 500-ml shaking flask. The media contain 0.5% methanol. For methanol, 30 micromolar Ca (Calcium chloride) plus Ho (Holmium chloride) was added. In log phase of the growth (OD600=0.3-0.4), the cells were harvested by centrifugation (9000 rpm, 4 C, 5 min), and frozen by liquid nitrogen. The cells were subjected to total RNA extraction with hot phenol extraction procedure (Babst et al., 1996, Mol Microbiol 19:827-839). Then ribosomal RNA was depleted by using Ribo-Zero rRNA removal kits (Gram-negative bacteria) (Epicentre) according to the manufacturer's protocol.
attributes.tag: sample_name
attributes.value: Methylobacterium_sp. 22A_RNAseq_CaHo
attributes.tag: strain
attributes.value: MA-22A