title: A
organism: Homo sapiens
description: Protocols: HAP1 cells were derived from the near-haploid chronic myeloid leukaemia cell line KBM7 as described (Carette et al., 2011). HAP1 cells were cultured in IMDM supplemented with 10% FBS, penicillin-streptomycin and L-glutamine. Cells were incubated at 37°C in 5% CO2 and grown to 80-90% confluence before harvesting total RNA Total RNA from 5 million cells was extracted using Qiagen RNeasy plus mini kit (74134). 1 μg of total RNA was subjected to library synthesis using the TruSeq RNA-Seq kit in technical replicate (e.g. two libraries were made from each RNA sample). 1 μg of total RNA was enriched for Poly-A mRNA using oligo dT-selection and reverse transcribed to cDNA. The cDNA was ligated with Illumina indexes. RNA libraries were prepared for sequencing using standard Illumina protocols.
attributes.tag: ENA first public
attributes.value: 2017-07-25
attributes.tag: ENA last update
attributes.value: 2017-07-11
attributes.tag: External Id
attributes.value: SAMEA104158618
attributes.tag: INSDC center alias
attributes.value: Stanford University
attributes.tag: INSDC center name
attributes.value: Stanford University
attributes.tag: INSDC first public
attributes.value: 2017-07-25T17:01:20Z
attributes.tag: INSDC last update
attributes.value: 2017-07-11T12:18:43Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-5865:A
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: HAP-1
attributes.tag: cell type
attributes.value: haploid cell
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: chronic myelogenous leukemia
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: sample name
attributes.value: E-MTAB-5865:A