title: Generic sample from Danio rerio
organism: Danio rerio
description: Maternal haploid embryos were generated according to standard procedure (Westerfield, 2000), using sperm from ÂgoldenÂ homozygous males  gol b1/b1 as a marker. DNA from sphere-stage maternal haploid, and sphere-stage control IVF diploids was extracted using phenol-chloroform and bisulfite converted using the EpiTect Bisulfite Kit (Qiagen, Valencia, CA 91355), followed by PCR amplification of 15 promoter regions. Genomic library was prepared according to Illumina's standard procedure and sequenced on Illumina MiSeq. Detailed experimental procedures and bioinformatics analysis can be found in the supplemental method section of the paper.
attributes.tag: strain
attributes.value: Tubingen
attributes.tag: label
attributes.value: PE: paired end, SE: single end
attributes.tag: development stage
attributes.value: sphere