title: BUlk DNA Sequencing of Parental and Resistant Cells of 451Lu
organism: Homo sapiens
description: Bulk Genomic Sequencing of Parental and Resistant Clones of 451Lu. Resistant Clone generated by treatment with 1uM of Vemurafenib
attributes.tag: isolate
attributes.value: The 451Lu cell line was derived from lung metastases of WM164 cells after subcutaneous injection into immunodeficient mice. WM164 is a metastatic human melanoma cell line established from a metastatic site (right upper arm) in a 22-year-old male with stage IV superficial spreading melanoma. 451Lu cell line features the specific V600E (Val600Glu) mutation at codon 600 in the BRAF gene.
attributes.tag: age
attributes.value: 22
attributes.tag: sex
attributes.value: male
attributes.tag: tissue
attributes.value: Right Upper Arm
attributes.tag: biomaterial_provider
attributes.value: Meenhard Herlyn, 3601 Spruce St, Philadelphia, PA 19104
attributes.tag: cell_line
attributes.value: 451Lu
attributes.tag: cell_type
attributes.value: Melanoma
attributes.tag: disease
attributes.value: Melanoma
attributes.tag: growth_protocol
attributes.value: DMEM with 10% FBS and 1% P/S for parental and DMEM with 10% FBS, 1% P/S and 1uM PLX-4720 for resistant cells
attributes.tag: growth_protocol_Parental
attributes.value: DMEM with 10% FBS and 1% P/S
attributes.tag: growth_protocol_Resistant
attributes.value: DMEM with 10% FBS, 1% P/S and 1uM PLX-4720
attributes.tag: BioSampleModel
attributes.value: Human