title: Quiescent RPE-1 cells, treated with 0.01mM ATM inhibitor and irradiated with 20J/cm^2 UV-C
organism: Homo sapiens
description: Human retina pigmented epithelium cells (RPE1) were grown to confluence and serum deprived for 72 hours to ensure quiescence. Cells were treated for 1hour with 0.01mM ATM inhibitor KU55933 and subsequently UV irradiated with 20J/m^2 UV-C (245nm). RNA was isolated 6hours post irradiation with the RNeasy kit (Qiagen) and PolyA RNA was isolated using the Dynabeads mRNA purification kit (Invitrogen). Sample integrity was verified with the Agilent 2100 Bioanalyzer. For each sample, cDNA library was prepared and validated using the Illumina TruSeq RNA sample preparation kit v2. Equal amounts of polyA RNA were chemically fragmented to approximately 350 nt. RNA-seq was performed according to the Illumina TruSeq v3 protocol on the HiSeq 2500 platform resulting in 100bp paired end reads.
attributes.tag: isolate
attributes.value: HTERT RPE-1
attributes.tag: age
attributes.value: -
attributes.tag: biomaterial_provider
attributes.value: ATCC
attributes.tag: sex
attributes.value: female
attributes.tag: tissue
attributes.value: Retina, eye; pigmented epithelium
attributes.tag: cell_line
attributes.value: HTERT RPE-1
attributes.tag: cell_type
attributes.value: Epithelial cells immortalized with hTERT
attributes.tag: culture_collection
attributes.value: ATCC:CRL-4000
attributes.tag: BioSampleModel
attributes.value: Human