title: C5_normal
organism: Homo sapiens
description: Protocols: Freshly collected tissues (6 cancers and matched normal mucosa samples) were processed to investigate their methylome. For methylation analysis, 1µg DNA was used for library preparation according to the Roche SeqCapEpi CpGiant protocol (Roche, Rotkreutz, Switzerland; details in Supplementary Experimental Procedures). Specifically with this method, DNA libraries were bisulfite converted prior to capture with a pre-designed probe pool (Roche-NimbleGen, Madison, WI) to enrich the methylation analysis to 80.5Mb of the genome, covering ~2.7 x 106 CpG sites (intra- and intergenic CpG islands, and gene promoters were thoroughly covered by this method, while CpG shores, enhancers and gene bodies were not as thoroughly investigated).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-11-23T17:04:22Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-06-29T15:28:58Z
attributes.tag: External Id
attributes.value: SAMEA4772482
attributes.tag: INSDC center name
attributes.value: UZH
attributes.tag: INSDC first public
attributes.value: 2018-11-23T17:04:22Z
attributes.tag: INSDC last update
attributes.value: 2018-06-29T15:28:58Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6949:C5_normal
attributes.tag: age
attributes.value: 81
attributes.tag: braf status
attributes.value: normal
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: colon segment involved
attributes.value: normal
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: colon cancer
attributes.tag: disease staging
attributes.value: normal
attributes.tag: individual
attributes.value: C5
attributes.tag: kras status
attributes.value: normal
attributes.tag: microsatellite status
attributes.value: normal
attributes.tag: organism part
attributes.value: colon
attributes.tag: sample name
attributes.value: E-MTAB-6949:C5_normal
attributes.tag: sampling site
attributes.value: normal tissue adjacent to neoplasm
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: male