title: CKO_IP_1
organism: Mus musculus
description: Protocols: Meis1/Hoxa9-transduced foetal liver c-Kit+ cells were collected after three rounds of serial re-plating in methylcellulose. Total RNA was extracted using TRIzol (Thermo Fisher Scientific). mRNA was purified from total RNA using PolyATtract mRNA Isolation Systems (Promega), fragmented and immunoprecipitated with anti-m6A antibody (Synaptic Systems, 202003). The library was constructed using TruSeq Stranded mRNA Sample Prep Kits (Illumina RS-122-2101)
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-04-05T04:04:05Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-03-13T16:06:22Z
attributes.tag: External Id
attributes.value: SAMEA5418682
attributes.tag: INSDC center name
attributes.value: MRC Centre for Regenerative Medicine, University of Edinburgh, Edinburgh, UK
attributes.tag: INSDC first public
attributes.value: 2019-04-05T04:04:05Z
attributes.tag: INSDC last update
attributes.value: 2019-03-13T16:06:22Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7783:CKO_IP_1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: Meis1/Hoxa9-transduced foetal liver c-Kit+ cells
attributes.tag: cell type
attributes.value: hematopoietic cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: 14.5 dpc
attributes.tag: disease
attributes.value: acute myeloid leukemia
attributes.tag: genotype
attributes.value: Ythdf2fl/fl; Vav-iCre
attributes.tag: organism part
attributes.value: hematopoietic system
attributes.tag: sample name
attributes.value: E-MTAB-7783:CKO_IP_1
attributes.tag: scientific_name
attributes.value: Mus musculus