title: Sample 47
organism: Mus musculus
description: Protocols: For end point sacrificing and sample collection, the mice were not fasted for longer than 2-3 hours due to the hypoglycemia associated with the mutant phenotype. All samples were collected at the same time of the day during the light period of the mice. The mice were anesthetized with pentobarbital, blood was collected into Li-heparin tubes by cardiac puncture, and plasma was separated. Organs were immediately dissected and snap frozen in liquid nitrogen or immersed in 10% buffered formalin solution. Approximately 1 mm3 pieces of tissue were fixed in 1.5% glutaraldehyde, 1.5% paraformaldehyde in 0.1 M Sörensen buffer pH 7.2 for electron microscopy. Standard RNA isolation protocol for total RNA was used For RNA sequencing, rRNA was removed and the sequencing libraries were prepared using Illumina TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Human/Mouse/Rat.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-11-16T17:03:33Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-11-15T17:39:40Z
attributes.tag: External Id
attributes.value: SAMEA5090669
attributes.tag: INSDC center name
attributes.value: DNA Sequencing and Genomics Lab, Institute of Biotechnology, HiLIFE, University of Helsinki
attributes.tag: INSDC first public
attributes.value: 2018-11-16T17:03:33Z
attributes.tag: INSDC last update
attributes.value: 2018-11-15T17:39:40Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7416:Sample 47
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: individual
attributes.value: 1414
attributes.tag: organism part
attributes.value: kidney
attributes.tag: sample name
attributes.value: E-MTAB-7416:Sample 47
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: C57BL/6JCrl