title: CTRL_0h_3
organism: Mus musculus
description: Protocols: Meis1/Hoxa9-transduced foetal liver c-Kit+ cells were collected after three rounds of serial re-plating in methylcellulose. Medium with 4SU was used for pre-leukemic cells labelling for 12 hours and was later replaced with 4SU-free medium (time 0). Cells were collected immediately after medium change and at 1, 3 and 9 hours. S4U-labeled RNA was treated with Iodoacetamine to alkylate 4-thiol groups. Total RNA was extracted using QIAzol Lysis Reagent (QIAGEN). Lexogen QuantSeq 3′ mRNA-Seq Library Prep Kit FWD for Illumina (cat. no. 015.24) was used for library construction according to manufacturer's instructions.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-04-05T04:04:05Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-03-18T09:21:43Z
attributes.tag: External Id
attributes.value: SAMEA5425576
attributes.tag: INSDC center name
attributes.value: MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, School of Biological Sciences, University of Edinburgh
attributes.tag: INSDC first public
attributes.value: 2019-04-05T04:04:05Z
attributes.tag: INSDC last update
attributes.value: 2019-03-18T09:21:43Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7784:CTRL_0h_3
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: hematopoietic cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: embryonic day 14.5
attributes.tag: disease
attributes.value: acute myeloid leukemia
attributes.tag: genotype
attributes.value: Ythdf2 fl/fl
attributes.tag: organism part
attributes.value: hematopoietic system
attributes.tag: phenotype
attributes.value: Meis1/Hoxa9-transduced foetal liver c-Kit+ cells
attributes.tag: replicate
attributes.value: 3
attributes.tag: sample name
attributes.value: E-MTAB-7784:CTRL_0h_3
attributes.tag: scientific_name
attributes.value: Mus musculus