organism: Clostridium thermobutyricum
attributes.tag: isolation_source
attributes.value: marine sediment
attributes.tag: collection_date
attributes.value: Not applicable
attributes.tag: geo_loc_name
attributes.value: North Sea: German Bight (Helgoland Mud Area)
attributes.tag: lat_lon
attributes.value: 54.052300 N 7.580400 E
attributes.tag: rel_to_oxygen
attributes.value: obligate anaerobe
attributes.tag: samp_mat_process
attributes.value: Enrichment was prepared using 5 mL volume from 4th transfer as starting material in a final volume of 50 mL widdel medium. Sample name identifies the unique information of each sample including the target 16S rRNA gene and the phase of the enrichment transfer.
attributes.tag: samp_size
attributes.value: 5 mL enrichment was centrifuged down to 1 mL volume which was used for DNA extraction
attributes.tag: source_material_id
attributes.value: Benzoate + magnetite enrichment at 30ºC after the fifth transfer completely metabolised benzoate coupled to magnetite reduction. 5mL was collected from the enrichment bottle and stored at -20ºC prior to DNA extraction and metagenome sequencing. Pooled DNA from three replicates of the BM5 enrichment used for metagenome sequencing. BM5_bin.13 is the reassembled metagenome assembled genome
attributes.tag: isolate
attributes.value: BM5_bin.13
attributes.tag: metagenomic
attributes.value: 1
attributes.tag: environmental-sample
attributes.value: 1
attributes.tag: sample-type
attributes.value: metagenomic assembly
attributes.tag: metagenome-source
attributes.value: marine sediment metagenome
attributes.tag: env_broad_scale
attributes.value: missing
attributes.tag: env_local_scale
attributes.value: missing
attributes.tag: env_medium
attributes.value: missing
attributes.tag: derived-from
attributes.value: This BioSample is a metagenomic assembly obtained from the marine sediment metagenome BioSamples: SAMN14820628, SAMN14820629, and SAMN14820630
attributes.tag: BioSampleModel
attributes.value: MIMAG