title: CTL_IP_1
organism: Mus musculus
description: Protocols: Meis1/Hoxa9-transduced foetal liver c-Kit+ cells were collected after three rounds of serial re-plating in methylcellulose. Total RNA was extracted using TRIzol (Thermo Fisher Scientific). mRNA was purified from total RNA using PolyATtract mRNA Isolation Systems (Promega), fragmented and immunoprecipitated with anti-m6A antibody (Synaptic Systems, 202003). The library was constructed using TruSeq Stranded mRNA Sample Prep Kits (Illumina RS-122-2101)
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-04-05T04:03:56Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-05-16T16:21:52Z
attributes.tag: External Id
attributes.value: SAMEA4666648
attributes.tag: INSDC center name
attributes.value: MRC Centre for Regenerative Medicine, University of Edinburgh, Edinburgh, UK
attributes.tag: INSDC first public
attributes.value: 2019-04-05T04:03:56Z
attributes.tag: INSDC last update
attributes.value: 2018-05-16T16:21:52Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6791:CTL_IP_1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: c-Kit+ cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: embryonic day 14.5
attributes.tag: genotype
attributes.value: Ythdf2 fl/fl; Meis1/Hoxa9 overexpression
attributes.tag: organism part
attributes.value: liver
attributes.tag: sample name
attributes.value: E-MTAB-6791:CTL_IP_1
attributes.tag: scientific_name
attributes.value: Mus musculus