title: Generic sample from Pinaceae
organism: Pinaceae
description: Genomic DNA was extracted from fresh needles or seed megagametophyte tissue using the FastDNA Kit  or the DNeasy Plant Mini Kit. Chloroplast genomes were amplified in 35 separate PCR reactions. For each species, the PCR reactions were quantified, pooled into equal-molar mixtures, and converted into barcoded Illumina sequencing libraries. Individual libraries were pooled into multiplex sequencing libraries ranging from 4X-6X.
attributes.tag: barcode AGCT
attributes.value: Abies firma ABIFIR01
attributes.tag: barcode CCT
attributes.value: Pinus contorta CONT40
attributes.tag: barcode ATT
attributes.value: Pinus merkusii MERK04
attributes.tag: barcode AAT
attributes.value: Pinus nelsonii NELS03
attributes.tag: barcode CTGT
attributes.value: Pinus torreyana ssp. torreyana TORR02