title: E7.7.284
organism: Homo sapiens
description: Protocols: Human embryos were obtained from two cohorts at the Huddinge Karolinska Hospital. The first cohort was from preimplantation genetic diagnosis (PGD) testing on embryonic day (E) 4 and cultured until E7 (expanded blastocyst, just prior to implantation) under standard conditions as performed in the IVF Clinic (5% CO2/5%O2 in IVF plates containing 700_l of CCMTM media (Vitrolife) covered with 300_l of OvoilTM (Vitrolife)). The second cohort was from frozen E2 embryos thawed (ThawKit� Cleave, VitroLife) and cultured in G-1� Plus media (VitroLife) until E3 and then transferred to CCM� media (VitroLife) from E3-E7. Embros were dissociated into single cells by the use of enzymatic (TrypLE) and mechanical separation, and then handpicked with fine glass capillaries by mouth pipetting. Single blastomemeres were directly dispensed into into individual wells of a 8-strip tube containing lysis buffer. Picelli et al, Full-length RNA-seq from single cells using Smart-seq2, Nature Protocols, 2014
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2016-04-06T17:01:25Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-09T05:44:14Z
attributes.tag: External Id
attributes.value: SAMEA3582156
attributes.tag: INSDC center name
attributes.value: Department of Cell and Molecular Biology, Karolinska Institutet
attributes.tag: INSDC first public
attributes.value: 2016-04-06T17:01:25Z
attributes.tag: INSDC last update
attributes.value: 2018-03-09T05:44:14Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-3929:E7.7.284
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell
attributes.value: 1383
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: embryonic day 7
attributes.tag: individual
attributes.value: E7.7
attributes.tag: organism part
attributes.value: embryo
attributes.tag: sample name
attributes.value: E-MTAB-3929:E7.7.284
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: single cell well quality
attributes.value: OK
attributes.tag: treatment
attributes.value: No