organism: Mus musculus
attributes.tag: isolate
attributes.value: Adult female mouse, bone marrow, cellular aliquot
attributes.tag: synthetic
attributes.value: FALSE
attributes.tag: sex
attributes.value: female
attributes.tag: age
attributes.value: 14 weeks
attributes.tag: age_event
attributes.value: not applicable
attributes.tag: population
attributes.value: not applicable
attributes.tag: ethnicity
attributes.value: not applicable
attributes.tag: race
attributes.value: not applicable
attributes.tag: strain
attributes.value: BALB/c
attributes.tag: linked_subjects
attributes.value: not applicable
attributes.tag: link_type
attributes.value: not applicable
attributes.tag: study_group_description
attributes.value: random sample
attributes.tag: disease
attributes.value: healthy
attributes.tag: disease_length
attributes.value: not applicable
attributes.tag: disease_stage
attributes.value: not applicable
attributes.tag: prior_therapies
attributes.value: not applicable
attributes.tag: immunogen
attributes.value: RSVF-F + Alum
attributes.tag: treatment
attributes.value: s.c. injection lateral flank
attributes.tag: medical_history
attributes.value: not applicable
attributes.tag: replicate
attributes.value: biological replicate 5
attributes.tag: sample_type
attributes.value: bone marrow
attributes.tag: tissue
attributes.value: bone marrow
attributes.tag: anatomic_site
attributes.value: Bone marrow from hindlegs (femur, tibia)
attributes.tag: health_state
attributes.value: healthy
attributes.tag: biomaterial_provider
attributes.value: IBI-STI LPDI, EPFL
attributes.tag: tissue_processing
attributes.value: Bone marrow cells were isolated from hindlegs by flushing bones with medium filled syringe until all bone marrow was isolated. A single cell suspension was then made by gently pressing bone marrow through cell strainer. Filter was washed, cells centrifuged and resuspended in 1.25 ml Trizol.
attributes.tag: cell_type
attributes.value: total bone marrow cells
attributes.tag: cell_phenotype
attributes.value: total bone marrow cells
attributes.tag: single_cell
attributes.value: False
attributes.tag: cell_number
attributes.value: not determined
attributes.tag: cells_per_reaction
attributes.value: not determined
attributes.tag: cell_storage
attributes.value: False
attributes.tag: cell_quality
attributes.value: not determined
attributes.tag: cell_isolation
attributes.value: no specific cell isolation
attributes.tag: cell_processing_protocol
attributes.value: Mouse bone marrow was isolated from hind legs (femur and tibia) and re-suspended in 1.5 ml Trizol (Life Technologies 15596) and stored at -80˚C until further processing. RNA extraction was performed using the PureLink RNA Mini Kit (Life Technologies 12183018A) following the manufacturer guidelines.
attributes.tag: BioSampleModel
attributes.value: Model organism or animal