title: E2A_si535_2
organism: Homo sapiens
description: Protocols: 697 cells (described here: https://www.dsmz.de/collection/catalogue/details/culture/ACC-42) were infected with shRNA lentivirus with 8 ug/mL polybrene in complete medium. The medium was replaced after 12 h of infection for the cells to recover for 36 h prior to 2 µg/mL puromycin selection for 48 h before harvesting the cells for RNA extraction. The shRNA used for knockdown are purchased from Dharmacon. Clone ID TRCN0000020389 and TRCN0000020392 were used for PBX1 knockdown. Clone ID TRCN0000017534 and TRCN0000017535 were used for E2A knockdown. 697 cells were cultured in RPMI 1640 (Invitrogen) and 10% FBS. RNA was extracted with RNeasy Mini Kit (Qiagen). Libraries were generated using the TruSeq RNA Sample Preparation Kit (Illumina).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-05-13T17:05:14Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-07-24T10:20:52Z
attributes.tag: External Id
attributes.value: SAMEA5807713
attributes.tag: INSDC center name
attributes.value: TMU Research Center of Cancer Translational Medicine, Taipei Medical University
attributes.tag: INSDC first public
attributes.value: 2020-05-13T17:05:14Z
attributes.tag: INSDC last update
attributes.value: 2019-07-24T10:20:52Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8179:E2A_si535_2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: 697
attributes.tag: cell type
attributes.value: pre-B cell
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: B-cell acute lymphoblastic leukemia
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: bone marrow
attributes.tag: sample name
attributes.value: E-MTAB-8179:E2A_si535_2
attributes.tag: scientific_name
attributes.value: Homo sapiens