title: Sample 2
organism: Mus musculus
description: Protocols: FGSCs at passage number 35-45 were cultured on gelatinized plates with minimum essential medium (MEM)-α (Gibco) containing 10% fetal bovine serum (Gibco), 1mM non-essential amino acids (Gibco) , 1mM sodium pyruvate (Gibco), 2mM L-glutamine (Gibco), 0.1mM β-mercaptoethanol (Sigma-Aldrich), 10ng/ml mouse epidermal growth factor (Pepreotech), 20ng/ml mouse leukemia inhibitory factor (Pepreotech), 10ng/ml human glial cell line-derived neurotrophic factor (Pepreotech), 10ng/ml human basic fibroblast growth factor (Pepreotech), and 100units/ml Penicillin-Streptomycin Solution at 37 °C in a 5 % CO2 atmosphere as previously described (Kang Z, et al. Nat Cell Biol. 2009). cells were covalently cross-linked by incubating cells in 1 % formaldehyde (Sigma-Aldrich) for 10 minutes at room temperature and incubated with 125 mM glycine for 5 minutes to quench cross-linking reaction. The fragmented chromatin fragments were immunoprecipitated with Protein A+G Magnetic beads (Millipore) coupled with anti-STAT3 antibody (13-7000, Invitrogen). After reverse crosslinking, ChIP and input DNA library were constructed with a NEBNext Ultra II DNA Library Prep Kit for Illumina (E7645, NEB).
attributes.tag: ENA first public
attributes.value: 2017-12-20
attributes.tag: ENA last update
attributes.value: 2017-11-10
attributes.tag: External Id
attributes.value: SAMEA104376754
attributes.tag: INSDC center alias
attributes.value: School of Biomedical Engineering, Shanghai Jiao Tong University
attributes.tag: INSDC center name
attributes.value: School of Biomedical Engineering, Shanghai Jiao Tong University
attributes.tag: INSDC first public
attributes.value: 2017-12-20T17:02:49Z
attributes.tag: INSDC last update
attributes.value: 2017-11-10T15:10:06Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6163:Sample 2
attributes.tag: age
attributes.value: 5
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: Female germline stem cell line
attributes.tag: common name
attributes.value: house mouse
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: female gonad
attributes.tag: sample name
attributes.value: E-MTAB-6163:Sample 2
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: C57BL/6