title: C
organism: Zindervirus SP6
description: SP6* was grown in continuous culture at 37C on E. coli K-12 host in LB broth for 307 hours, then equal multiplicities of the final population and the initial phage, SP6*, were coinfected to allow recombination.  The mixture was grown by serial passage for 5 hours and the final evolved population was used to isolate DNA for sequencing.