title: SM7_uc2
organism: Homo sapiens
description: Protocols: The plasma was prepared from EDTA blood by centrifugation at 1,0006g for 15 minutes to separate the plasma and blood cells. Total RNA from plasma was extracted from 100 ml of the sample using the miRNeasy kit (Qiagen, Valencia, CA). The quality and quantity of the RNA were evaluated with Agilent 2100 Bioanalyzer (Santa Clara, CA) and NanoDrop 1000 spectrophotometer (Thermo Scientific, Wilmington, DE). In average, we obtained about 100 ng of RNA per ml of plasma. As a control we also obtained total RNA from Ambion (Life Technologies, Carlsbad, CA). NextGen sequencing libraries were prepared with small RNA sample preparation kits from Illumina (Illumina, San Diego, CA).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2013-03-29T17:01:40Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T16:20:53Z
attributes.tag: External Id
attributes.value: SAMEA1714521
attributes.tag: INSDC center name
attributes.value: Institute for Systems Biology
attributes.tag: INSDC first public
attributes.value: 2013-03-29T17:01:40Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T16:20:53Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-1568:SM7_uc2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: human
attributes.tag: disease state
attributes.value: ulcerative colitis
attributes.tag: organism part
attributes.value: plasma
attributes.tag: sample name
attributes.value: E-MTAB-1568:SM7_uc2
attributes.tag: scientific_name
attributes.value: Homo sapiens