title: Adeno-­associated virus (AAV) serotype 2 capsid (cap) gene directed evolution; VP heptapeptide-1 library-3 (Lib-3)
organism: adeno-associated virus 2
description: The project is aimed at identifying many heptapeptide amino acid sequences within the adeno-associated virus (AAV) serotype 2 VP protein  (i.e., VP1 amino acid positions from 319 to 325) that are viable for virion formation. Such viable heptapeptides are selected from a pool of random nucleotides coding random heptapeptides by means of directed evolution. VP heptapeptide-1 library-3 (Lib-3) is a library of PCR amplicons coding heptapeptides (amino acid positions from 319 to 325) in a VP heptapeptide mutant library that has undergone three rounds of positive selection.
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