title: CR099_(p)ppGpp0_starv_t0_2
organism: Corynebacterium glutamicum ATCC 13032
description: Protocols: After reaching an OD value of 2.5, cells were harvested by rapid centrifugation, supernatant removal, and immediate shock freezing in liquid nitrogen. Samples of stressed cells were treated identically. C. glutamicum CR099 and C. glutamicum CR099 relrelSrelH cells were grown in CGXII medium with 10 g/L glucose and 50 mg/L of all 20 proteinogenic amino acids with an initial optical density of 0.4. Cultures of 40 mL were incubated in 250 mL Erlenmeyer flasks at 30 °C and 300 rpm (2 inch deviation). RNA-isolation was performed using the RNeasy Mini Kit along with the DNase Kit (both Qiagen), using three DNA digestion steps. For initial cell disruption the cell pellet was resuspended in 800 µL RLT-buffer (containing 10 µL/mL ?-mercaptoethanol), transferred into lysing matrix B tubes (MP Biomedicals) and homogenized for 30 sec at 6500 rpm in a Precellys® 24 homogeniser (Peqlab). Illumina TruSeq® Stranded mRNA low sample protocol starting with rRNA-depleted total RNA
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-12-02T17:03:46Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-06-18T14:19:18Z
attributes.tag: External Id
attributes.value: SAMEA5722493
attributes.tag: INSDC center name
attributes.value: Microbial Genomics and Biotechnology, Center for Biotechnology, Bielefeld University
attributes.tag: INSDC first public
attributes.value: 2019-12-02T17:03:46Z
attributes.tag: INSDC last update
attributes.value: 2019-06-18T14:19:18Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8070:CR099_(p)ppGpp0_starv_t0_2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: genotype
attributes.value: CR099 rel, relS, relH
attributes.tag: growth condition
attributes.value: exponential phase
attributes.tag: organism part
attributes.value: whole organism
attributes.tag: sample name
attributes.value: E-MTAB-8070:CR099_(p)ppGpp0_starv_t0_2
attributes.tag: scientific_name
attributes.value: Corynebacterium glutamicum ATCC 13032