title: siNT rep3
organism: Homo sapiens
description: Protocols: Cells were cultured in 37deg incubator with 5% CO2. Cells were treated with 100pmol siRNA using RNAiMAX for 72 hours. RNA was collected using Qiagen RNeasy kit. Cells were lysed directly in the plate. RNA-seq libraries were constructed using Illumina TruSeq Stranded mRNA kit.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-03-09T04:03:25Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-10-16T16:15:26Z
attributes.tag: External Id
attributes.value: SAMEA6087378
attributes.tag: INSDC center name
attributes.value: UNIVERSITY OF MANCHESTER
attributes.tag: INSDC first public
attributes.value: 2020-03-09T04:03:25Z
attributes.tag: INSDC last update
attributes.value: 2019-10-16T16:15:26Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8446:siNT rep3
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: OE19
attributes.tag: cell type
attributes.value: epithelial cell
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: esophageal adenocarcinoma
attributes.tag: organism part
attributes.value: esophagus
attributes.tag: sample name
attributes.value: E-MTAB-8446:siNT rep3
attributes.tag: scientific_name
attributes.value: Homo sapiens