title: WT.Rep3
organism: Saccharomyces cerevisiae
description: Protocols: For each strain, three independent colonies were used to prepare samples of total RNA. Wild-type H4 and otherwise isogenic T80A H4 cells were grown in yeast extract, peptone, glucose, adenine sulfate (YPDA) to an optical density at 660 nm (OD660) of 0.6. Total RNA was isolated through hot-phenol extraction. RNA was further subjected to DNase I treatment (catalog number E1009-A; Zymo Research) and poly-A mRNA was purified using poly-T oligo-attached magnetic beads (NEXTflex Poly(A) Beads, catalog number NOVA-512981) according to the manufacturer's instructions. The quantity and purity of RNA were measured by using an Agilent high-sensitivity RNA screen tape system (catalog number 5067-5579; Agilent Technologies) and Qubit (Molecular Probes, Invitrogen). Libraries were prepared for sequencing from 200 ng of polyA mRNA by using the NEXTflex RNA-seq kit (NOVA-512913, Illumina) according to the manufacturer's instructions. Samples were barcoded and combined at uniform molarity to create a single pool for sequencing.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-09-05T17:03:05Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-07-30T11:20:33Z
attributes.tag: External Id
attributes.value: SAMEA4809032
attributes.tag: INSDC center name
attributes.value: University of Portsmouth
attributes.tag: INSDC first public
attributes.value: 2018-09-05T17:03:05Z
attributes.tag: INSDC last update
attributes.value: 2018-07-30T11:20:33Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7090:WT.Rep3
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: baker's yeast
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: growth condition
attributes.value: YPDA medium
attributes.tag: sample name
attributes.value: E-MTAB-7090:WT.Rep3
attributes.tag: scientific_name
attributes.value: Saccharomyces cerevisiae