title: Sample 48
organism: Rattus norvegicus
description: Protocols: PCCl3 were maintained in Coon's modified F12 medium (EuroClone) supplemented with 5% newborn bovine serum (HyClone Laboratories) and six hormones 16. Cells were exposed for 7 days to CPF (6x10-7 M, 6x10-8 M, 6x10-9 M, Greyhound Chromatography F2057) and ETU (6x10-8 M, 6x10-9 M, 6x10-10 M, 2-Imidazolidinethione, Sigma-Aldrich I504) and their combinations. Total RNA was isolated from treated and control cells using Trizol reagent (Invitrogen) following manufacturer's instructions. Total RNA samples were treated with DNasi (NEB), purified with RNasy Mini Kit (Qiagen) and controlled with Agilent 2100 bioanalyzer (Agilent Technologies). RNAs, 3 µg/each obtained from three biological replicates, were pooled to minimize sample-to-sample variations. 1μg of each RNA pool was used for library preparation with TruSeq Stranded total RNA Sample Prep Kit (Illumina Inc.) according to the manufacturer's instructions.
attributes.tag: ENA first public
attributes.value: 2016-12-06
attributes.tag: ENA last update
attributes.value: 2016-11-24
attributes.tag: External Id
attributes.value: SAMEA4557165
attributes.tag: INSDC center alias
attributes.value: IRGS, Biogem
attributes.tag: INSDC center name
attributes.value: IRGS, Biogem
attributes.tag: INSDC first public
attributes.value: 2016-12-06T17:02:11Z
attributes.tag: INSDC last update
attributes.value: 2016-11-24T10:53:27Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-5269:Sample 48
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: PCCl3
attributes.tag: cell type
attributes.value: thyrocyte
attributes.tag: common name
attributes.value: Norway rat
attributes.tag: organism part
attributes.value: thyroid gland
attributes.tag: sample name
attributes.value: E-MTAB-5269:Sample 48