title: Bulk of individuals from STABILISING selection on LOW NITRATE replicate B - repeat
organism: Arabidopsis thaliana
attributes.tag: isolate
attributes.value: Pool of all ~200 individuals from the last generation of selection (generation 10) from STABILISING selection on LOW NITRATE replicate B - repeat to achieve >100x depth of coverage
attributes.tag: dev_stage
attributes.value: over stage 3 (see https://www.arabidopsis.org/portals/education/growth.jsp)
attributes.tag: geo_loc_name
attributes.value: United Kingdom: Cambridge
attributes.tag: tissue
attributes.value: leaf (plant ontology term PO:0025034)
attributes.tag: growth_protocol
attributes.value: In the last generation of selection we collected self seed from all 200 individuals of each of the 18 populations. We then pooled together 5 seeds from each (total of 1000 seeds per population) and grew them on compost and a size hole punch of leaf tissue was used as material for DNA. All leaf samples were ground together for DNA extraction. In this way, each of the original 200 individuals should have contributed a similar amount of tissue to the final pool.
attributes.tag: population
attributes.value: Population derived from the Arabidopsis MAGIC lines, themselves derived from 19 accessions
attributes.tag: sample_type
attributes.value: Pooled DNA
attributes.tag: BioSampleModel
attributes.value: Plant