title: Generic sample from Macaca mulatta
organism: Macaca mulatta
description: We dissected postmortem cerebellar cortex samples from five male rhesus macaques (8-40 years old).   Total RNA was extracted from dissections by Trizol reagent (Invitrogen, Carlsbad, CA) and treated for 30 min at 37 degree centigrade with RNase free DNase I (Ambion, Austin, TX). RNA was purified with the RNeasy MinElute Kit according to the manufacturer's instructions (Qiagen, Valencia, CA). This procedure depletes RNA molecules with length shorter than 200 nt.  Resulting RNA samples from five rhesus macaque individuals was mixed in equal proportions resulting in one pooled samples. 10ug RNA was treated with two rounds of RiboMinus kit (Invitrogen) to remove most of the Ribosome RNA. The cDNA libraries were prepared starting from 2ug of rRNA-Reduced total RNA per sample and using random hexamer primers (Invitrogen, Cat. No. 48190-011). It has to be noted that the resulting double-stranded cDNA fragments do not preserve information about the strand specificity of the original transcript. The Illumina sequencing libraries were prepared according to the single-end sample preparation protocol (http://www.illumina.com). The libraries were sequenced using the 1G Illumina Genome Analyzer. The sequencing products were the single-end 36 nucleotides (nt) long sequence reads.