title: PIK39_exp_SS
organism: Helicobacter pylori P12
description: PIK39 (deltaHPP12_1377::aphA).Exponential phase. strand-specific mRNA-seq. This strain was grown in Brucella both containing 10% FBS up to mid-exponential phase (O.D.600 =0.4). Then, cells were harvested for RNA extraction. Total RNA was extracted from cell pellets using PureLink RNA mini kit (Ambion). rRNA was depleted using Ribo-Zero rRNA Removal kit for Gram-negative bacteria (Epicentre). cDNA library was constructed using SureSelect - Strand specific RNA library kit (Agilent Technologies). The indexed cDNA libraries were pooled and sequenced in HiSeq2500 platform (Illumina).
attributes.tag: sample_name
attributes.value: PIK39_exp_SS