title: CCHiC-HeLaS3CCL2p2-G1-0.25FA
organism: Homo sapiens
description: Protocols: HeLa cells were grown in standard DMEM media with sodium pyruvate (Gibco #11995073) with 10 % Bovine serum (Gibco #16170078), 1% PenStrep (Gibco #15140) and 1% Antibiotic-Antimycotic agent (Gibco # 15240). HFF cells were grown in standard DMEM media with sodium pyruvate (Gibco #11995073) with 10 % FBS (BenchMark #100-101), 1% PenStrep (Gibco #15140) and 1% Antibiotic-Antimycotic agent (Gibco # 15240). K562 cells were maintained in standard RPMI-1640 media with 2 mM L-glutamine (Gibco #22400-089) with 10% FBS (BenchMark #100-101), 1% PenStrep (Gibco #15140) and 1% Antibiotic-Antimycotic agent (Gibco # 15240). To collect HeLs cells arrested in S-phase, cells were synchronized by double Thymidine block (2mM thymidine in grouth media). HeLa cells in G1 phase were obtained by double thymidine block followed by release into normal growth media for 12h (early G1) or 14h (middle G1). To collect mitotic cells, HeLa, HFF-1 and K562 cells were synchronized by double Thymidine block followed by release into normal growth media for several hous. Then cells were arrested in Metaphase by adding nocodazole at 100ng/mkl final concentration in grouth medai as described in (Naumova et al. 2013) Hi-C was performed following (Lieberman and van Berkum et al., Science, 2009) with modifications detailed in (Naumova et al., 2013).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2015-03-19T17:02:34Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T23:44:58Z
attributes.tag: External Id
attributes.value: SAMEA3309442
attributes.tag: INSDC center name
attributes.value: Harvard University
attributes.tag: INSDC first public
attributes.value: 2015-03-19T17:02:34Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T23:44:58Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-1948_2:CCHiC-HeLaS3CCL2p2-G1-0.25FA
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell cycle stage
attributes.value: G1mid
attributes.tag: cell line
attributes.value: HeLa
attributes.tag: common name
attributes.value: human
attributes.tag: sample name
attributes.value: E-MTAB-1948_2:CCHiC-HeLaS3CCL2p2-G1-0.25FA
attributes.tag: scientific_name
attributes.value: Homo sapiens