title: Hmga2-/- Ctrl SUPT16H
organism: Mus musculus
attributes.tag: source_name
attributes.value: Mouse embryonic fibroblasts
attributes.tag: cell type
attributes.value: Immortalized Mouse embryonic fibroblasts
attributes.tag: strain
attributes.value: C57BL/6
attributes.tag: tissue
attributes.value: whole embryo
attributes.tag: age
attributes.value: embryonic day 15.5
attributes.tag: genotype
attributes.value: Hmga-/-
attributes.tag: cell extraction
attributes.value: Timed-pregnant mice were sacrificed on post coitum day 15.5 according to standard methods. The embryos were collected in sterile PBS complemented with 100 U\ml penicillin and 100 U\ml streptomycin. After removing the head, limbs, tail, viscera, liver and heart, the remaining body was trypsinized with 1x trypsin-EDTA solution (0.05 %) for 20 minutes at 37°C and single cells were obtained via physical disruption by sterile Pasteur pipet. The cells from each embryo were diluted with growth medium containing FCS and plated separately.
attributes.tag: antibody
attributes.value: SPT16 (Cell Siganling, 12191)