title: dil_D5_2_18S-1/10^5-diluted-diversity-sample-repetition1
organism: soil metagenome
description: A silt-clay loam soil was collected from Mas d�Imbert, France (for soil characteristics, see Offre et al. 2007 and Zancarini et al. 2012). Major part of this soil was sterilized by 224 kGy Gamma irradiation (Ionisos, Sabl�-sur-Sarthe, France), while the resting part was used for the preparation of the cascade dilutions of inocula. To that end, 10 g of dried soil was mixed to 50 mL� of sterilized water and gently shacked during 30 minutes, corresponding to the suspension hereafter called �High microbial diversity level�. Then, following the protocol described in Maron et al. (2018), five serial 10-fold dilutions in demineralized water were prepared from the previous one, and the highest dilution at 10-5 was chosen for the study, hereafter called �Low microbial diversity level�. The two suspensions corresponding to high and low microbial diversity levels were then inoculated to the sterilized soil, at a rate of 130 mL of suspension per pot in order to obtain a moisture level of 55% of soil water holding capacity. All the pots were then covered by a parafilm containing 5 holes in order to maintain the moisture level while allowing a good aeration of the soil, and incubated in the dark in a growth chamber at 20�C during 42 days.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-07-12T04:04:22Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-03-14T09:45:30Z
attributes.tag: External Id
attributes.value: SAMEA5418789
attributes.tag: INSDC center name
attributes.value: INRA-CMSE
attributes.tag: INSDC first public
attributes.value: 2019-07-12T04:04:22Z
attributes.tag: INSDC last update
attributes.value: 2019-03-14T09:45:30Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: dil_D5_2_18S
attributes.tag: collection date
attributes.value: 2017
attributes.tag: environment (biome)
attributes.value: terrestrial biome
attributes.tag: environment (feature)
attributes.value: anthropogenic geographic feature
attributes.tag: environment (material)
attributes.value: soil
attributes.tag: geographic location (country and/or sea)
attributes.value: France
attributes.tag: geographic location (depth)
attributes.value: 0.2
attributes.tag: geographic location (elevation)
attributes.value: 57
attributes.tag: geographic location (latitude)
attributes.value: 43.802998
attributes.tag: geographic location (longitude)
attributes.value: 4.963057
attributes.tag: investigation type
attributes.value: metagenome
attributes.tag: project name
attributes.value: 2015-BQE_Marion_Prudent
attributes.tag: sample name
attributes.value: dil_D5_2_18S
attributes.tag: scientific_name
attributes.value: soil metagenome
attributes.tag: sequencing method
attributes.value: 454-pyrosequencing
attributes.tag: soil environmental package
attributes.value: soil