title: Sample 10
organism: Arabidopsis thaliana
description: Protocols: Samples for RNA isolation and RNA-seq analysis were harvested on the day 20 and the day 25 after sowing at 11:00 AM. Plants were grown under controlled conditions in the growth chamber at 22 °C with 65% relative humidity, a light intensity of 110 µmol m-2 s-1, and the photoperiod of 16 hours light and 8 hours dark. No treatment was applied For RNA sequencing, RNA was isolated using TriFastTM (Peqlab, Erlangen, Germany) following the manufactures instructions after homogenization of the plant material. Plant material was lysed in 1 ml TriFastTM reagent per 100 mg plant tissue. After vortex and incubation for 5 minutes at RT. 200 µl chloroform was added and the solution was shaken for 15 seconds and incubated for 5 minutes at RT. After centrifugation at 4700 rpm, the aqueous phase containing RNA was separated and transferred to a new tube. Precipitation of RNA was done by adding 500 µl isopropanol. After centrifugation at 4700 rpm at 4 ˚C, the RNA pellet was washed with 70% ethanol. The isolated RNA was stored in at -80 ˚C. Strand-specific cDNA library
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-05-14T04:05:26Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-04-09T20:45:08Z
attributes.tag: External Id
attributes.value: SAMEA6656314
attributes.tag: INSDC center name
attributes.value: UNIVERSITY OF KIEL
attributes.tag: INSDC first public
attributes.value: 2020-05-14T04:05:26Z
attributes.tag: INSDC last update
attributes.value: 2020-04-09T20:45:08Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8912:Sample 10
attributes.tag: age
attributes.value: 20
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: thale cress
attributes.tag: developmental stage
attributes.value: inflorescence development stage
attributes.tag: ecotype
attributes.value: ws-4
attributes.tag: genotype
attributes.value: poco1 mutant FLAG_465F03
attributes.tag: organism part
attributes.value: rosette leaf
attributes.tag: sample name
attributes.value: E-MTAB-8912:Sample 10
attributes.tag: scientific_name
attributes.value: Arabidopsis thaliana