title: Flower-replicate-1
organism: Arabidopsis thaliana
description: Protocols: Seeds were planted in pots containing commercial potting mix with fertilizers. Pots were covered with clear perforated plastic wrap and kept at 4 degrees celsius for 3 days to break the dormancy. After 3 days plants were transferred to the Intellus Ultra growth chamber (Percival Scientific, IA, USA) which was set to temperature 22-23 degrees celsius, light intensity 120-150 micromol/m2sec under the cycle of 16h light and 8h dark. Soil was kept moist by gently spraying with water every 72 hours to maintain humidity to 50-60%. Sampling time point is given in days after germination. Total RNA from frozen samples was extracted as a method described in Filichkin et al., 2010. Total RNA was used to isolate large RNA as per manufacturer's protocol for miRNeasy Mini kits (Qiagen Inc., USA), and RNase-free DNase (Life Technologies Inc., USA). True-Seq kit (Illumina Inc.) was used to prepare RNA-seq libraries, according to the manufacturer's protocol.
attributes.tag: ENA first public
attributes.value: 2019-07-11
attributes.tag: ENA last update
attributes.value: 2022-05-04
attributes.tag: External Id
attributes.value: SAMEA104551701
attributes.tag: INSDC center alias
attributes.value: OREGON STATE UNIVERS
attributes.tag: INSDC center name
attributes.value: Oregon State University
attributes.tag: INSDC first public
attributes.value: 2019-07-11T04:02:29Z
attributes.tag: INSDC last update
attributes.value: 2022-05-04T00:19:32Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6422:Flower-replicate-1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: thale cress
attributes.tag: developmental stage
attributes.value: flowering stage
attributes.tag: ecotype
attributes.value: Col-0
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: flower
attributes.tag: sample name
attributes.value: E-MTAB-6422:Flower-replicate-1
attributes.tag: sampling time point
attributes.value: 39