title: Adult ad libitum 1
organism: Mus musculus
description: Male C57BL/6JN and B6D2F1 mice fed either ad libitum or CR were obtained from the aging colony maintained by the National Institute on Aging (NIA).  After receiving the mice from NIA were housed at the animal facility and maintained under SPF conditions and individually housed in a HEPA barrier environment at the University of Oklahoma Health Sciences Center for at 4-8 weeks before used in the following experiments. Mice fed ad libitum were fed irradiated NIH-31 mouse/rat diet (Teklad, Envigo), and the CR mice were fed the same diet fortified for micronutrients. CR was initiated by NIA at 14 weeks of age of 10% restriction, increased to 25% restriction at 15 weeks, and to 40% restriction at 16 weeks of age. Adult (9 months of age) fed ad libitum (AL) or CR and old mice (24 months of age) fed AL or CR were fasted overnight, and samples were collected during sacrifice (n=8-10/group).
attributes.tag: strain
attributes.value: C57BL/6
attributes.tag: age
attributes.value: 9 months
attributes.tag: sex
attributes.value: male
attributes.tag: tissue
attributes.value: Colon mucosa
attributes.tag: treatment
attributes.value: ad libitum diet
attributes.tag: replicate
attributes.value: AAL1
attributes.tag: BioSampleModel
attributes.value: Model organism or animal