title: Desert soil RNA, reverse transcribed with TGIRT at 58°C.
organism: bacterium
description: A sample from the upper 10cm of soil, collected after discarding a biological soil crust. Sample was collected from under a canopy of a desert shrub Hammada scoparia. Total RNA was extracted and reverse transcribed with TGIRT at 58°C.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-07-11T17:04:15Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-06-02T10:33:20Z
attributes.tag: External Id
attributes.value: SAMEA5683281
attributes.tag: INSDC center name
attributes.value: BEN GURION UNIVERSITY OF THE NEGEV
attributes.tag: INSDC first public
attributes.value: 2019-07-11T17:04:15Z
attributes.tag: INSDC last update
attributes.value: 2019-06-02T10:33:20Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: Negev043
attributes.tag: ammonium
attributes.value: 4.03 mg/kg
attributes.tag: ammonium method
attributes.value: Potassium chloride extract
attributes.tag: amount or size of sample collected
attributes.value: 0.5
attributes.tag: collection date
attributes.value: 2016-10
attributes.tag: composite design/sieving (if any)
attributes.value: sieved with 2mm sieve, samples compiled from 2 adjacent blocks
attributes.tag: current land use
attributes.value: pristine
attributes.tag: environment (biome)
attributes.value: arid desert
attributes.tag: environment (feature)
attributes.value: plateau
attributes.tag: environment (material)
attributes.value: soil
attributes.tag: geographic location (altitude)
attributes.value: 500
attributes.tag: geographic location (country and/or sea)
attributes.value: Israel
attributes.tag: geographic location (depth)
attributes.value: 0.05
attributes.tag: geographic location (elevation)
attributes.value: 500
attributes.tag: geographic location (latitude)
attributes.value: 34°80′
attributes.tag: geographic location (longitude)
attributes.value: 30°86′
attributes.tag: geographic location (region and locality)
attributes.value: Negev desert
attributes.tag: investigation type
attributes.value: bacteria_archaea
attributes.tag: library reads sequenced
attributes.value: 55986
attributes.tag: nitrate
attributes.value: 5.7 mg/kg
attributes.tag: nitrate method
attributes.value: Soil aqueous extract
attributes.tag: nucleic acid amplification
attributes.value: https://doi.org/10.1016/j.pedobi.2019.02.001
attributes.tag: nucleic acid extraction
attributes.value: https://doi.org/10.1038/protex.2012.046
attributes.tag: pcr primers
attributes.value: 341F-ACACTGACGACATGGTTCTACACCTACGGGAGGCAGCAG; 806R-TACGGTAGCAGAGACTTGGTCTGGACTACHVGGGTWTCTAAT
attributes.tag: ph
attributes.value: 8.1
attributes.tag: ph method
attributes.value: saturated soil extract
attributes.tag: phosphorus
attributes.value: 22.2 mg/kg
attributes.tag: phosphorus method
attributes.value: Sodium bicarbonate extract
attributes.tag: profile position
attributes.value: toeslope
attributes.tag: project name
attributes.value: RTcomparison
attributes.tag: salinity
attributes.value: 1.22 dS/m
attributes.tag: salinity method
attributes.value: Saturated soil extract
attributes.tag: sample collection device or method
attributes.value: sterilized spatula
attributes.tag: sample material processing
attributes.value: homogenization with sieving,
attributes.tag: sample name
attributes.value: Negev043
attributes.tag: sample volume or weight for DNA extraction
attributes.value: 0.6
attributes.tag: sample weight for DNA extraction
attributes.value: 0.6
attributes.tag: scientific_name
attributes.value: bacterium
attributes.tag: sequencing method
attributes.value: Illumina MiSeq
attributes.tag: soil environmental package
attributes.value: soil
attributes.tag: soil type
attributes.value: Calcisol
attributes.tag: storage conditions (fresh/frozen/other)
attributes.value: Freezed at -80°C
attributes.tag: target gene
attributes.value: 16S rRNA
attributes.tag: total organic matter
attributes.value: 0.71 %
attributes.tag: total organic matter method
attributes.value: Dichromate oxidation
attributes.tag: water content
attributes.value: 1.708
attributes.tag: water content method
attributes.value: gravimetric