title: Sample 71
organism: Homo sapiens
description: Protocols: Single cell FACS of tetramer-enriched PBMCs Single tetramer+ cells were sorted into 96-well plates containing 0.5 μl dNTP mix (10 mM), 0.5 μl oligo-dT pri- mer (5 μM), and 1 μl lysis buffer (prepared by adding 1 μl RNase inhibitor to 19 μl Triton X-100 solution, 0.2% v/v). Libraries were prepared using the Smart-seq2 protocol with the following modifications. cDNA synthesis and pre-amplification reaction volumes were halved to 5 and 12.5 μl, respectively. The final concentration of the ERCC RNA Spike-In Mix in the reverse-transcription step was 1:400 million.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-02-27T17:03:37Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-01-11T16:56:31Z
attributes.tag: External Id
attributes.value: SAMEA5215642
attributes.tag: INSDC center name
attributes.value: School of Medical Sciences and The Kirby Institute, UNSW
attributes.tag: INSDC first public
attributes.value: 2019-02-27T17:03:37Z
attributes.tag: INSDC last update
attributes.value: 2019-01-11T16:56:31Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7606:Sample 71
attributes.tag: age
attributes.value: 31
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: CD8-positive, alpha-beta T cell
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: individual
attributes.value: KK51
attributes.tag: organism part
attributes.value: blood
attributes.tag: phenotype
attributes.value: A2/PB1 413 tetramer positive
attributes.tag: sample name
attributes.value: E-MTAB-7606:Sample 71
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: male