title: 293FlpIN_pcDNA5_Xrn2_WT_Dox_Trip_20_Spt5
organism: Homo sapiens
attributes.tag: source_name
attributes.value: HEK293 Flp-in T-REx pcDNA5 HA-Xrn2 WT shXrn2
attributes.tag: cell line
attributes.value: HEK293
attributes.tag: growth conditions
attributes.value: DMEM 10% FCS, +hygromycin, +blasticidin, +doxycycline
attributes.tag: antibody
attributes.value: rabbit anti Spt5 (Glover-Cutter et al NSMB 15:71-78, 2008)
attributes.tag: treatment
attributes.value: Xrn2 expression was induced with 2ug/ml doxycycline for 24 hrs, Triptolide (10 mM) was added for 20 min.