title: 15.9 Î¼M glucose , 250 Î¼atm CO2
organism: marine metagenome
description: Partial (V1-V2) 16S rDNA amplicons from 0.2 Î¼m-filtered bacterial assemblages present on the last experimental day (Day 9) in a Fram Strait surface seawater mesocosm experiment conducted on the deck of the research vessel G.O. Sars from 20-29 June 2009. 16S rDNA amplicons from each of six mesocosms were barcoded with unique 6 nt barcodes for identification, then pooled and sequenced in one direction in a single run on a Roch/454 GS FLX platform. Raw sequences were quality-checked and trimmed using the RDP-II pyrosequencing pipeline. Chimeras were removed using the Black Box Chimera Check (B2C2; Gontcharova et al 2010, Open Microbiol J 4, 47-52) program.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2013-04-05T17:02:42Z
attributes.tag: ENA-KEYWORD
attributes.value: GSC, MIxS, MIMARKS
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-09T09:56:54Z
attributes.tag: External Id
attributes.value: SAMEA1710142
attributes.tag: Geographic location (country:region,area)
attributes.value: Fram Strait
attributes.tag: INSDC center name
attributes.value: UIB-UNIRES-MILJO
attributes.tag: INSDC first public
attributes.value: 2013-04-05T17:02:42Z
attributes.tag: INSDC last update
attributes.value: 2018-03-09T09:56:54Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: P3
attributes.tag: amount or size of sample collected
attributes.value: 1000
attributes.tag: chimera check
attributes.value: 6
attributes.tag: collection date
attributes.value: 2009-06-29
attributes.tag: depth
attributes.value: 0-0.5
attributes.tag: elevation
attributes.value: 0
attributes.tag: enviornmental package
attributes.value: water
attributes.tag: environment (biome)
attributes.value: oceanic sea surface microlayer biome ENVO:01000034
attributes.tag: environment (featurel)
attributes.value: cold surface current ENVO:01000102
attributes.tag: environment (material)
attributes.value: ocean water ENVO:00002151
attributes.tag: geographic location (latitude)
attributes.value: 76.93
attributes.tag: geographic location (longitude)
attributes.value: 3.58 W
attributes.tag: investigation type
attributes.value: MIxS
attributes.tag: pcr primers
attributes.value: 8F: 5'-BxxxxxxAGAGTTTGATCMTGGCTCAG-3', 357R: 5'-AxxxxxxCTGCTGCCTYCCGTA-3'
attributes.tag: ph
attributes.value: 8.121
attributes.tag: project name
attributes.value: MERCLIM (NFR contract 184860/S30)
attributes.tag: sample name
attributes.value: P3
attributes.tag: scientific_name
attributes.value: marine metagenome
attributes.tag: sequencing method
attributes.value: Pyrosequencing
attributes.tag: target gene
attributes.value: 16S rRNA