organism: sea anemone metagenome
attributes.tag: project_name
attributes.value: Preliminary assessment of Edwardsiella andrillae Microbiome
attributes.tag: submitted_to_insdc
attributes.value: NCBI-SRA
attributes.tag: investigation_type
attributes.value: miens-survey
attributes.tag: geo_loc_name
attributes.value: Antarctica: Coulman High Site, Ross Ice Shelf
attributes.tag: lat_lon
attributes.value: 77.5266667 S 171.3350000 E
attributes.tag: collection_date
attributes.value: 2010-12
attributes.tag: env_biome
attributes.value: ice shelf
attributes.tag: env_feature
attributes.value: ice shelf bottom
attributes.tag: env_material
attributes.value: ice and seawater
attributes.tag: env_package
attributes.value: host-associated
attributes.tag: host
attributes.value: Edwardsiella andrillae
attributes.tag: subspecf_gen_lin
attributes.value: bacteria
attributes.tag: source_mat_id
attributes.value: Anemone.E.A.3
attributes.tag: oxy_stat_samp
attributes.value: aerobic
attributes.tag: samp_collect_device
attributes.value: ROV SCINI
attributes.tag: samp_mat_process
attributes.value: preserved in ethanol
attributes.tag: samp_size
attributes.value: 1 individual
attributes.tag: nucl_acid_ext
attributes.value: http://www.ncbi.nlm.nih.gov/pubmed/8979338
attributes.tag: nucl_acid_amp
attributes.value: A single-step 30 cycle PCR using HotStarTaq Plus Master Mix Kit (Qiagen, Valencia, CA) were used under the following conditions: 94oC for 3 minutes, followed by 28 cycles of 94oC for 30 seconds; 53oC for 40 seconds and 72oC for 1 minute; after which a final elongation step at 72oC for 5 minutes was performed. Following PCR, all amplicon products from different samples were mixed in equal concentrations and purified using Agencourt Ampure beads (Agencourt Bioscience Corporation, MA, USA). Samples were sequenced utilizing Roche 454 FLX titanium instruments and reagents and following manufacturer’s guidelines
attributes.tag: target_gene
attributes.value: 16S rRNA
attributes.tag: target_subfragment
attributes.value: V1-2
attributes.tag: seq_meth
attributes.value: 454 pyrosequencing
attributes.tag: pcr_primers
attributes.value: 27Fmod:GRGTTTGATCMTGGCTCAG, 519R:GWATTACCGCGGCKGCTG
attributes.tag: pcr_cond
attributes.value: initial denaturation: 94degC_3min; 28 cycles: denaturation=94degC_30sec, annealing=53degC_40sec, elongation=72degC_1min; final elongation: 72degC_5min
attributes.tag: depth
attributes.value: 256 m
attributes.tag: host_common_name
attributes.value: Anemone
attributes.tag: host_taxid
attributes.value: 1821226
attributes.tag: host_subject_id
attributes.value: E.A.3
attributes.tag: host_life_stage
attributes.value: Adult
attributes.tag: host_body_site
attributes.value: whole specimen
attributes.tag: host_substrate
attributes.value: ice
attributes.tag: samp_salinity
attributes.value: 34.573 PSU
attributes.tag: std_deviation_of_salinity
attributes.value: 0.017
attributes.tag: oxy_stat_samp
attributes.value: aerobic
attributes.tag: temp
attributes.value: -1.949 degree Celcius
attributes.tag: std_deviation_of_temp
attributes.value: 0.053
attributes.tag: samp_store_temp
attributes.value: 4 degree Celcius
attributes.tag: samp_store_dur
attributes.value: 5 years
attributes.tag: diss_react_phos
attributes.value: 65 mg m^-3
attributes.tag: nitrate_as_nitro
attributes.value: 419 mg m^-3
attributes.tag: BioSampleModel
attributes.value: MIMARKS.survey
attributes.tag: BioSampleModel
attributes.value: MIGS/MIMS/MIMARKS.host-associated