title: EE501S1_Susceptible_Bulk
organism: Solanum chacoense
description: 10 Solanum chacoense F2 individuals derived from a cross between S. chacoense line USDA8380-1 and S. chacoense M6 susceptible to Colorado potato beetle defoliation, as determined by replicated field trial. A hole punch was taken from each leaflet of each genotype and pooled prior to DNA extraction.
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: cultivar
attributes.value: not applicable
attributes.tag: ecotype
attributes.value: not applicable
attributes.tag: age
attributes.value: 16 weeks
attributes.tag: dev_stage
attributes.value: mature
attributes.tag: geo_loc_name
attributes.value: not collected
attributes.tag: tissue
attributes.value: terminal leaflet, third fully expanded leaf
attributes.tag: biomaterial_provider
attributes.value: Douches Lab, 1066 Bogue Street, East Lansing MI 48824
attributes.tag: collected_by
attributes.value: Natalie Kaiser
attributes.tag: collection_date
attributes.value: 2017-11-08
attributes.tag: genotype
attributes.value: Solanum chacoense
attributes.tag: growth_protocol
attributes.value: In vitro plants of each genotype were grown in a growth chamber (20°C, 16-hr photoperiod, 70% relative humidity)
attributes.tag: lat_lon
attributes.value: 22.3 N 33.5 W
attributes.tag: phenotype
attributes.value: Colorado potato beetle susceptible
attributes.tag: population
attributes.value: F2
attributes.tag: sample_type
attributes.value: Bulked leaf tissue sample
attributes.tag: BioSampleModel
attributes.value: Plant