title: Sample 12
organism: Mus musculus
description: Protocols: RNAseq was performed on tumors extracted from TAg+ mice fed control, low sugar, high protein, 10% fat, 30% fat, high glucose, or sugar-free diets. Mice were assessed for tumour formation by palpation and dissected tumours were selected for similar size before sample freezing. Total RNA was isolated using RNeasy Mini kit (Qiagen). RNA concentration and purity were determined spectrophotometrically using the Nanodrop ND-1000 (Nanodrop Technologies) and RNA integrity was assessed using a Bioanalyser 2100 (Agilent). 3′mRNA-seq library preparation and transcriptome analysis was performed using the QuantSeq 3′mRNA-Seq Library Prep Kit for Illumina and QuantSeq data analysis workflow.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-07-16T04:05:51Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-08-08T15:20:29Z
attributes.tag: External Id
attributes.value: SAMEA5858306
attributes.tag: INSDC center name
attributes.value: Laboratory of Angiogenesis and Vascular Metabolism VIB-KU Leuven Center for Cancer Biology (CCB)
attributes.tag: INSDC first public
attributes.value: 2020-07-16T04:05:51Z
attributes.tag: INSDC last update
attributes.value: 2019-08-08T15:20:29Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8227:Sample 12
attributes.tag: age
attributes.value: 101
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: disease
attributes.value: pancreatic cancer
attributes.tag: genotype
attributes.value: Ela1-TAg
attributes.tag: organism part
attributes.value: pancreas
attributes.tag: sample name
attributes.value: E-MTAB-8227:Sample 12
attributes.tag: sampling site
attributes.value: neoplasm
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: C57BL/6