title: General Sample for Chlamys farreri
organism: Azumapecten farreri
description: Embryos (blastulae and gastrulae), larvae (trochophore and D-shaped larvae) and adults (the total soft tissues, adductor muscle, male and female gonads) of C. farreri were collected.All the samples were flash frozen in liquid nitrogen and stored at -80°C until use. Total RNA was separately extracted from each sample by following the protocol previously described in Hu et al. (2006). Equal amount of RNA from blastulae, gastrulae, trochophore larvae and D-shaped larvae was mixed as the embryo and larval RNA pool. Five 454 libraries were prepared by following the protocol as described in Meyer et al. (2009). The embryo and larval library and the total soft tissues library were normalized using the Trimmer-Direct cDNA normalization kit (Evrogen, Moscow, Russia) to decrease the prevalence of abundant transcripts. Libraries for adductor muscle, male and female gonads were not normalized in order to facilitate the identification of candidate tissue-specific transcripts. During the construction of each library, adaptors with a unique barcode (a 3-base sequence) were ligated to both ends of the cDNAs to distinguish the sequencing reads from those of other libraries. Approximately, 5 μg of the mixed libraries was sequenced using the Roche Genome Sequencer FLX system (Roche, Basel, Switzerland).
attributes.tag: label
attributes.value: A library representing diverse life stages and adult tissues of C. farreri