title: Oci-ly10 AzaPBA
organism: Homo sapiens
description: Protocols: The DLBCL cell lines used were SU-DHL4, SU-DHL6, SU-DHL10, SU-DHL16 and RC-K8 of GC DLBCL subtype and U-2932, Oci-Ly3, Oci-Ly10, SU-DHL2, SU-DHL5 and RIVA of ABC DLBCL as well as SU-DHL7 (unclassified). Cell lines were maintained at 37 degrees C, 5%CO2 in a humidified atmosphere in RPMI or IMDM (RIVA, Oci-Ly10) supplemented with 10% (Oci-Ly10, RIVA, SU-DHL2 and SU-DHL5) or 20% heat-inactivated FBS and antibiotics. CD19 positive B cells were isolated from buffy coats of healthy volunteer blood donors obtained from the blood donation centre Zurich (ZHBSD). The quality of the isolated RNA was determined with a Qubit® (1.0) Fluorometer (Life Technologies, California, USA) and a Bioanalyzer 2100 (Agilent, Waldbronn, Germany). Only those samples with a 260 nm/280 nm ratio between 1.8-2.1 and a 28S/18S ratio within 1.5-2 were further processed. The TruSeq RNA Sample Prep Kit v2 (Illumina, Inc, California, USA) was used in the succeeding steps. Briefly, total RNA samples (100-1000 ng) were poly A enriched and then reverse-transcribed into double-stranded cDNA. The cDNA samples was fragmented, end-repaired and polyadenylated before ligation of TruSeq adapters containing the index for multiplexing Fragments containing TruSeq adapters on both ends were selectively enriched with PCR. The quality and quantity of the enriched libraries were validated using Qubit® (1.0) Fluorometer and the Caliper GX LabChip® GX (Caliper Life Sciences, Inc., USA). The product is a smear with an average fragment size of approximately 260 bp. The libraries were normalized to 10nM in Tris-Cl 10 mM, pH8.5 with 0.1% Tween 20.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2015-03-25T17:01:39Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T21:16:28Z
attributes.tag: External Id
attributes.value: SAMEA2768224
attributes.tag: INSDC center name
attributes.value: University of Zurich
attributes.tag: INSDC first public
attributes.value: 2015-03-25T17:01:39Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T21:16:28Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-2925:Oci-ly10 AzaPBA
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: Oci-ly10
attributes.tag: common name
attributes.value: human
attributes.tag: sample name
attributes.value: E-MTAB-2925:Oci-ly10 AzaPBA
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: female