title: PIK14_exp
organism: Helicobacter pylori 26695
description: PIK14  (deltaHP_0260::aphA) were 26695 derivative strains. Exponential phase. strand-nonspecific mRNA-seq. This strain was grown in Brucella both containing 10% FBS up to mid-exponential phase (O.D.600 =0.4). Then, cells were harvested for RNA extraction. Total RNA was extracted from cell pellets using PureLink RNA mini kit (Ambion). rRNA was depleted using Ribo-Zero rRNA Removal kit for Gram-negative bacteria (Epicentre). cDNA library was constructed using TruSeq RNA library prep kit (Illumina). The indexed cDNA libraries were pooled and sequenced in HiSeq2500 platform (Illumina).
attributes.tag: sample_name
attributes.value: PIK14_exp
attributes.tag: strain
attributes.value: 26695
attributes.tag: sub_strain
attributes.value: PIK14