title: SCA1_human cerebellum
organism: Homo sapiens
description: Protocols: Post-mortem human cerebellum from a 74-year-old female SCA1 patient and a age/sex-matched healthy individual were obtained from the MRC London Neurodegerative Diseases Brain Bank. Total RNA was extracted from frozen samples using the Direct-zol RNA Kit (Zymo Research). An aliquot of total RNA was loaded on Total RNA Pico Chip and measured for its integrity using Bioanalyzer 2100 (Agilent Technologies, USA). Sequencing libraries were prepared using KAPA RNA HyperPrep with Riboerase Kit, according to manufacture's instructions (Roche, USA).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-12-02T04:02:46Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-02-19T14:28:41Z
attributes.tag: External Id
attributes.value: SAMEA5360472
attributes.tag: INSDC center name
attributes.value: Institute of Applied Biosciences/Centre for Research and Technology Hellas, Thessaloniki, Greece
attributes.tag: INSDC first public
attributes.value: 2019-12-02T04:02:46Z
attributes.tag: INSDC last update
attributes.value: 2019-02-19T14:28:41Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7714:SCA1_human cerebellum
attributes.tag: age
attributes.value: 74
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: Spinocerebellar ataxia type 1
attributes.tag: organism part
attributes.value: cerebellum
attributes.tag: sample name
attributes.value: E-MTAB-7714:SCA1_human cerebellum
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: female