title: Generic sample from Strombus gigas
organism: Aliger gigas
description: Total RNA was isolated from 12 individual conch tissue samples (neural ganglia, gonad, digestive gland from each of four conchs) using TRIzol (Invitrogen, Carlsbad, CA, USA) following the manufacturer's protocol; 200 ng each RNA sample were pooled and used as the template for the cDNA library construction and normalization using the SMART (Clontech/Takara Bio USA, Madison, WI, USA) and Trimmer (Evrogen Joint Stock Company, Moscow, Russia) kits, respectively.