title: Ioni-7
organism: marine metagenome
description: marine water sample in the Ionian Sea collected with 12 l Niskin bottles at the DCM depth
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2013-04-15T17:02:35Z
attributes.tag: ENA-KEYWORD
attributes.value: GSC, MIxS
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-09T10:01:36Z
attributes.tag: External Id
attributes.value: SAMEA1877300
attributes.tag: Geographic location (country:region,area)
attributes.value: Ionian Sea
attributes.tag: INSDC center name
attributes.value: ESE
attributes.tag: INSDC first public
attributes.value: 2013-04-15T17:02:35Z
attributes.tag: INSDC last update
attributes.value: 2018-03-09T10:01:36Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: Ioni-7_ITS
attributes.tag: adapters
attributes.value: 5'‐CGTATCGCCTCCCTCGCGCCATCAG‐MID‐FORWARD-specific-sequence-3' and 5'‐CTATGCGCCTTGCCAGCCCGCTCAG‐MID‐REVERSE-specific-sequence-3'
attributes.tag: amount or size of sample collected
attributes.value: 168 litres
attributes.tag: broker name
attributes.value: EMG
attributes.tag: collection date
attributes.value: 2010-01-10
attributes.tag: depth
attributes.value: 77
attributes.tag: elevation
attributes.value: 77
attributes.tag: enviornmental package
attributes.value: water
attributes.tag: environment (biome)
attributes.value: ENVO:01000004
attributes.tag: environment (featurel)
attributes.value: ENVO:00000016
attributes.tag: environment (material)
attributes.value: ENVO:00002149
attributes.tag: geographic location (latitude)
attributes.value: 36.4827
attributes.tag: geographic location (longitude)
attributes.value: 15.6494
attributes.tag: investigation type
attributes.value: MIxS
attributes.tag: multiplex identifiers
attributes.value: CGAGAGATAC
attributes.tag: nucleic acid amplification
attributes.value: PCR
attributes.tag: nucleic acid extraction
attributes.value: Total DNA was purified from filters cut in small pieces using the PowerSoil DNA Isolation Kit (MoBio, USA). Nucleic acids were resuspended in 10 mM Tris-HCl, pH 8.
attributes.tag: pcr conditions
attributes.value: initial denaturation 94degC_3min; 25 cycles of denaturation:94degC_15sec/annealing:52degC_30sec/elongation:72degC_1min; final elongation 72degC_15min
attributes.tag: pcr primers
attributes.value: 5′-CAATYTGTTGCAGRGCGMAAGC-3'(forward) and 5′-AGCAATAGAAGGGCATCTA-3'(reverse)
attributes.tag: project name
attributes.value: Tintinnid 454
attributes.tag: sample collection device or method
attributes.value: 12 litres Niskin bottles
attributes.tag: sample material processing
attributes.value: Samples filtered to keep the >20 µm size fraction of the biomass
attributes.tag: sample name
attributes.value: Ioni-7_ITS
attributes.tag: sample storage duration
attributes.value: 1 month
attributes.tag: sample storage temperature
attributes.value: -20
attributes.tag: scientific_name
attributes.value: marine metagenome
attributes.tag: sequencing method
attributes.value: Pyrosequencing