title: Miwi2-asEGFP#6-4
organism: Mus musculus
description: Protocols: Mice were grown under the following condition: temperature 23.5 degree centigrade +/-2 degree , humidity 50 +/-10 % and 12 hour illumination (light period 8:00-20:00) The mouse tail was treated with Protease K and RNase A. The equal amount of Phenol/Chloroform solution was added. After centrifugation, the sup was harvested and then the equal amount of isopropanol was addicted. The DNA pellet was centrifugated and washed with 70% ethanol. The genomic DNAs were dried up at 55 degree centigrade. The DNAs were dilluted with TE. The extracted DNAs were fragmented to a length of 150 -200 nt by Covaris. These DNAs were amplified and concentrated with SureSelect XT Target Enrichment System (Agilent technologies) according to the manufacture's protocol. The bait RNAs corresponding to Miwi2 Promoter sequence were synthesized by Hokkaido System Science.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2015-03-18T17:03:50Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T22:58:26Z
attributes.tag: External Id
attributes.value: SAMEA3226107
attributes.tag: INSDC center name
attributes.value: Osaka University
attributes.tag: INSDC first public
attributes.value: 2015-03-18T17:03:50Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T22:58:26Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-3257:Miwi2-asEGFP#6-4
attributes.tag: age
attributes.value: 8 week
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: Miwi2-asEGFP#6
attributes.tag: organism part
attributes.value: tail
attributes.tag: sample name
attributes.value: E-MTAB-3257:Miwi2-asEGFP#6-4
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: strain
attributes.value: BDF1 background