title: Sample 4
organism: Mus musculus
description: Protocols: The mouse stomach corpus was dissected and the tissue was dissociated into single-cell suspension by the incubation with Dispase ‖ (45U.ml). Then more than 9000 cells for each sample were sorted directly into 300 ul of lysis buffer (Arcturus Pico Pure RNA Isolation Kit) using MOFLO system. Cell lysate was snap-frozen on dry ice and subsequently stored at -80 °C. RNA purification was performed using the Arcturus Pico Pure RNA Isolation Kit (Applied Biosystems) following the manufacturer's instructions. The integrity of the total RNA was confirmed using an Agilent BioAnalyzer. cDNA libraries wre generated from total RNA according to QuantSeq 3' mRNA-Seq FWD library preparation protocol.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-05-14T04:02:24Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-06-01T17:03:51Z
attributes.tag: External Id
attributes.value: SAMEA4704755
attributes.tag: INSDC center name
attributes.value: WT-MRC Cambridge Stem Cell Institute, The University of Cambridge
attributes.tag: INSDC first public
attributes.value: 2019-05-14T04:02:24Z
attributes.tag: INSDC last update
attributes.value: 2018-06-01T17:03:51Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6850:Sample 4
attributes.tag: age
attributes.value: 9
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: stem cell of gastric corpus gland
attributes.tag: common name
attributes.value: house mouse
attributes.tag: genotype
attributes.value: Gt(ROSA)26Sor<tm1(CAG-Venus/GMNN,Cherry/CDT1)Jkn>
attributes.tag: organism part
attributes.value: stomach corpus
attributes.tag: phenotype
attributes.value: mVenus+ve
attributes.tag: sample name
attributes.value: E-MTAB-6850:Sample 4
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: C57BL/6