organism: skin metagenome
description: Larval sablefish skin microbiome. Collected by swabbing fish skin after fish were collected from rearing tanks. DNA extracted using the Qiagen DNeasy PowerSoil kit and used for PCR amplification of 16S rRNA gene (V3-4) with primers 341/806R. PCR products were pair-end sequenced (2 x 300 bp) on an Illumina MiSeq.
attributes.tag: host
attributes.value: sablefish, Anoplopoma fimbria
attributes.tag: isolation_source
attributes.value: Puget Sound, Washington, USA
attributes.tag: collection_date
attributes.value: 2016-03-10
attributes.tag: geo_loc_name
attributes.value: USA: NOAA research laboratory, Manchester, Washington
attributes.tag: lat_lon
attributes.value: 47.571199 N 122.552665 W
attributes.tag: source_material_id
attributes.value: 3_10_16_Tank15_FishSkin_16S
attributes.tag: BioSampleModel
attributes.value: Metagenome or environmental