title: Bulk of individuals from DIRECTIONAL selection on HIGH NITRATE replicate A
organism: Arabidopsis thaliana
attributes.tag: isolate
attributes.value: Pool of all ~200 individuals from the last generation of selection (generation 10) from DIRECTIONAL selection on HIGH NITRATE replicate A
attributes.tag: dev_stage
attributes.value: 10 day seedling
attributes.tag: geo_loc_name
attributes.value: United Kingdom: Cambridge
attributes.tag: tissue
attributes.value: whole plant (plant ontology term PO:0005679)
attributes.tag: growth_protocol
attributes.value: In the last generation of selection we collected self seed from all 200 individuals of each of the 18 populations. We then pooled together 5 seeds from each (total of 1000 seeds per population) and grew them in liquid media for 10 days. We used 50ml liquid ATS media, shaken at 40RPM in a tissue culture room with 16 hour light and a temperature of 22/18 ºC during day/night cycles. After 10 days, all seedlings were harvested and ground together for DNA extraction. In this way, each of the original 200 individuals should have contributed a similar amount of tissue to the final pool.
attributes.tag: population
attributes.value: Population derived from the Arabidopsis MAGIC lines, themselves derived from 19 accessions
attributes.tag: sample_type
attributes.value: Pooled DNA
attributes.tag: BioSampleModel
attributes.value: Plant