title: B. xylophilus isolate from Japan
organism: Bursaphelenchus xylophilus
description: B. xylophilus from Japan (1 isolate), was grown in fungal culture (Botrytis cinerea. The total RNA was isolated from mixed stage cultures with the Trizol method. cDNA was synthesized with MINT cDNA synthesis kit (Evrogen, Moscow, Russia) to amplify selectively mRNA through polyA tails, and normalized according to the Duplex-Specific Nuclease-technology, following the instructions of the TRIMMER cDNA Normalization kit (Evrogen, Moscow, Russia). 454 pyrosequencing of each normalized library was performed according to 454 standard procedures.