title: Structure-seq2 (-DMS) Rep2 [Custom_primer_B]
organism: Oryza sativa Japonica Group
attributes.tag: source_name
attributes.value: Shoot
attributes.tag: cultivar
attributes.value: Nipponbare
attributes.tag: tissue
attributes.value: shoot
attributes.tag: age
attributes.value: 14 days
attributes.tag: treatment
attributes.value: -DMS
attributes.tag: rt denaturation
attributes.value: 90 °C with salt
attributes.tag: rt reaction
attributes.value: 55 °C
attributes.tag: biotin added
attributes.value: no
attributes.tag: rt purification
attributes.value: PAGE
attributes.tag: ligation method
attributes.value: T4 DNA ligase
attributes.tag: ligation purification
attributes.value: PAGE
attributes.tag: pcr enzyme
attributes.value: Q5
attributes.tag: final purification
attributes.value: PAGE
attributes.tag: sequencing primer
attributes.value: custom
attributes.tag: cultivar
attributes.value: japonica