title: 0.1% DMSO treatment
organism: Homo sapiens
description: Protocols: Hu5/KD3 cells were seeded 5×10^4 cells per collagenⅠcoated 24-well plate, cultured with DMEM (High Gluc.) containing 20% FBS, 2% UltroserG and supplemented with penicillin(100 units/mL), streptomycin (100 units/mL), Amphotericin B (250 ng/mL), L-Glu (2 mmol/L). Next day, started to treat with 0.1% DMSO or 10 μM DAPT in 10% FBS/DMEM and cultured three days. Total RNA was extracted as one sample from three wells by using RNeasy mini kit (QIAGEN). bcl2fastq2 (v2.17) software used for basecalling.Sequenced reads were mapped to hg19 whole genome using STAR software (ver. 2.5.2b) with parameters -twopassMode Basic, -outFilterIntronMotifs RemoveNoncanonical, -sjdbOverhang 99, -sjdbGTFfile.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-03-07T04:06:35Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-02-26T13:26:14Z
attributes.tag: External Id
attributes.value: SAMEA6591589
attributes.tag: INSDC center name
attributes.value: Department of Molecular Therapy National Institute of Neuroscience National Center of Neurology and Psychiatry
attributes.tag: INSDC first public
attributes.value: 2020-03-07T04:06:35Z
attributes.tag: INSDC last update
attributes.value: 2020-02-26T13:26:14Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8825:0.1% DMSO treatment
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: Hu5/KD3
attributes.tag: cell type
attributes.value: myoblast
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: normal
attributes.tag: growth condition
attributes.value: 10% FBS/ DMEM
attributes.tag: sample name
attributes.value: E-MTAB-8825:0.1% DMSO treatment
attributes.tag: scientific_name
attributes.value: Homo sapiens