title: Tet-on YFP-ATXN1(Q82) MSCs_D5_C
organism: Homo sapiens
description: Protocols: Samples include technical triplicates of Tet-On YFP-ATXN1(Q82) MSCs cultured in the absence (D0) or presence of Dox for 2, 5 or 10 days (D2, D5 or D10, respectively) and technical replicates of a Venus-tagged MSC line, stably producing a fluorescent variant of YFP. Total RNA was extracted from samples using the AllPrep DNA/RNA/miRNA Universal Kit (QIAGEN, Hilden, Germany) according to manufacture's protocol. mRNA was enriched from 250ng total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). Sequencing libaries were prepared from mRNA using SENSE Total RNA-seq Library Prep Kit (Lexogen, Austria), according to manufacture's protocol.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-12-02T04:02:46Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-02-19T15:13:32Z
attributes.tag: External Id
attributes.value: SAMEA5360506
attributes.tag: INSDC center name
attributes.value: Institute of Applied Biosciences/Centre for Research and Technology Hellas, Thessaloniki, Greece
attributes.tag: INSDC first public
attributes.value: 2019-12-02T04:02:46Z
attributes.tag: INSDC last update
attributes.value: 2019-02-19T15:13:32Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7713:Tet-on YFP-ATXN1(Q82) MSCs_D5_C
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: stem cell derived cell line
attributes.tag: cell type
attributes.value: mesenchymal stem cell
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: Spinocerebellar ataxia type 1
attributes.tag: genetic modification
attributes.value: Inducible overexpression of human ataxin-1(Q82)
attributes.tag: genotype
attributes.value: Tet-on YFP-ATXN1(Q82)
attributes.tag: sample name
attributes.value: E-MTAB-7713:Tet-on YFP-ATXN1(Q82) MSCs_D5_C
attributes.tag: scientific_name
attributes.value: Homo sapiens