organism: Escherichia coli K-12
description: Protocols: RNA from WT and arcA mutant of APEC XM, cultured in duck serum, and RNA from WT and arcA mutant, grown in LB broth at 37°C under microaerobic conditions RNA from WT and arcA mutant of APEC XM, cultured in duck serum, and RNA from WT and arcA mutant, grown in LB broth, was extracted using an RNeasy minikit (Promega) with a 15 mins on-column DNase digestion (Promega) according to the manufacturer's instructions. The cDNA libraries were constructed from 1,000 ng of total RNA and rRNA was removed using the MICROBExpress™ Bacterial mRNA Enrichment Kit (Life Technologies, Carlsbad, CA). The sequencing libraries were prepared using a TruSeq Stranded total RNA sample preparation kit (Illumina, San Diego, CA) following the guidelines of the manufacturers. Multiplex libraries were prepared using barcoded primers and a median insert size of 250 bp. Libraries were analyzed for size distribution using a Bioanalyzer and quantified by quantitative RT-PCR using a Kapa library quantification kit (Kapa Biosystems, Boston, MA), and relative volumes were pooled accordingly.
attributes.tag: ENA first public
attributes.value: 2017-03-01
attributes.tag: ENA last update
attributes.value: 2015-03-10
attributes.tag: External Id
attributes.value: SAMEA3292127
attributes.tag: INSDC center alias
attributes.value: Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University
attributes.tag: INSDC center name
attributes.value: Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University
attributes.tag: INSDC first public
attributes.value: 2017-03-01T17:07:06Z
attributes.tag: INSDC last update
attributes.value: 2015-03-10T14:09:31Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-3396:APEC Mutant serum 2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: genotype
attributes.value: mutant
attributes.tag: growth condition
attributes.value: Serum
attributes.tag: sample name
attributes.value: E-MTAB-3396:APEC Mutant serum 2
attributes.tag: strain
attributes.value: Avian pathogenic E. coli