organism: Candida parapsilosis
description: Protocols: The strain CPL2H1 and two homozygous mutants otf1/otf1 and gtf1/gtf1 derived from this strain have the background of Candida parapsilosis CLIB214 (CBS604). The strains CPL2H1 (LH), CPL2H1∆gtf1/∆gtf1 and CPL2H1∆otf1/∆otf1 were pre-cultivated in an SD medium (0.67% [wt/vol] yeast nitrogen base w/o amino acids (Difco), 2% [wt/vol] glucose) for 24 h at 28 °C, then washed in water, inoculated (OD600 ~ 0.2) into an SMix medium (0.67% [wt/vol] yeast nitrogen base w/o amino acids (Difco), 5 mM 3-hydroxybenzoate, 5 mM 4-hydroxybenzoate and 5 mM hydroquinone) and grown for additional 24 h at 28 °C. The pre-cultures were inoculated (OD600 ~ 0.3-0.4) into an SMix medium and cultivated at 28 °C till OD600 ~ 1. All media were supplemented with leucine (20 μg/ml) and histidine (20 μg/ml). Total RNA was extracted using the hot acid phenol protocol essentially as described in Collart and Oliviero (2001) and purified using an Uneasy mini kit (Qiagen); (RIN 8.6 - 9.1). Reference: Collart, M.A. and Oliviero, S. (2001) Preparation of yeast RNA. Curr. Protoc. Mol. Biol. Chapter 13: Unit 13.12. The sequencing library was prepared using a TruSeq Stranded mRNA LT Sample Prep Kit (2x 151).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2021-01-15T04:09:30Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-08-17T14:14:49Z
attributes.tag: External Id
attributes.value: SAMEA7192629
attributes.tag: INSDC center name
attributes.value: Department of Computer Science Faculty of Mathematics, Physics and Informatics Comenius University in Bratislava, Slovakia
attributes.tag: INSDC first public
attributes.value: 2021-01-15T04:09:30Z
attributes.tag: INSDC last update
attributes.value: 2020-08-17T14:14:49Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-9442:CPL2H1_otf1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: genotype
attributes.value: CPL2H1 delta otf1/delta otf1
attributes.tag: organism part
attributes.value: whole organism
attributes.tag: sample name
attributes.value: E-MTAB-9442:CPL2H1_otf1
attributes.tag: scientific_name
attributes.value: Candida parapsilosis
attributes.tag: strain
attributes.value: CLIB 214 (CBS 604)