organism: Mus musculus
description: Protocols: Cells were isolated from a pool of six mouse proximal small intestines (1cm of length each) at E16.5 using collagenase treatment and cell sorting as described in the previous section. 20000 DAPInegCD31negCD45negEpCAMpos cells were processed using 10x Genomics Chromium protocols. RNA was converted into cDNA and amplified as part of the 10x v2 chemistry. Libraries were constructed using the 10x v2 chemistry.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-05-15T04:03:51Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-02-25T10:43:34Z
attributes.tag: External Id
attributes.value: SAMEA5374488
attributes.tag: INSDC center name
attributes.value: Biotech Research and Innovation Centre, University of Copenhagen
attributes.tag: INSDC first public
attributes.value: 2019-05-15T04:03:51Z
attributes.tag: INSDC last update
attributes.value: 2019-02-25T10:43:34Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7660:Sample 1
attributes.tag: age
attributes.value: embryonic day 16.5
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: intestinal epithelial cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: embryo
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: individual
attributes.value: mixed pool of 6 fetuses
attributes.tag: organism part
attributes.value: small intestine
attributes.tag: phenotype
attributes.value: DAPI-, CD31-, CD45-, EpCAM+
attributes.tag: sample name
attributes.value: E-MTAB-7660:Sample 1
attributes.tag: sampling site
attributes.value: proximal small intestine
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: mixed
attributes.tag: strain
attributes.value: C57BL/6NRj