organism: Phaseolus vulgaris
description: Plasmids were introduced into Agrobacterium rhizogenes K599 strain, and used for plant transformation in independent experiments with 15 plants each. Plants were maintained for hairy root formation for 2 weeks and we obtained from 2 to 6 independent TdT fluorescence positive roots per plant, which were pooled in each independent experiment used for total RNA isolation.
attributes.tag: cultivar
attributes.value: Pinto Saltillo
attributes.tag: ecotype
attributes.value: missing
attributes.tag: age
attributes.value: 5 weeks
attributes.tag: geo_loc_name
attributes.value: Mexico: Cuernavaca, Morelos
attributes.tag: tissue
attributes.value: transgenic hairy roots
attributes.tag: biomaterial_provider
attributes.value: Dr. Jose Luis Reyes, Instituto de Biotecnología, UNAM
attributes.tag: collected_by
attributes.value: Biol. Guadalupe Sosa-Valencia
attributes.tag: collection_date
attributes.value: 2014-10
attributes.tag: growth_protocol
attributes.value: Estrada-Navarrete G, Alvarado-Affantranger X, Olivares JE, Guillen G, Diaz-Camino C, Campos F, Quinto C, Gresshoff PM, Sanchez F. 2007. Fast, efficient and reproducible genetic transformation of Phaseolus spp. by Agrobacterium rhizogenes. Nat Protoc 2, 1819-1824.
attributes.tag: sample_type
attributes.value: hairy roots
attributes.tag: temp
attributes.value: 24 C
attributes.tag: BioSampleModel
attributes.value: Plant