organism: Mus musculus
description: Protocols: Crypts were isolated from the small intestines of Bach2 KO and control mice (n=3, respectively) 10 days after tamoxifen administration. Total RNA was extracted for sequencing using RNA-Seq.RNA degradation and contamination was monitored on 1% agarose gels. RNA purity was checked using the NanoPhotometer® spectrophotometer (IMPLEN, CA, USA).A total amount of 3ug RNA per sample was used as input material for the RNA sample preparations. Sequencing libraries were generated using NEBNext® (NEB, USA) following manufacture's recommendations and index codes were added to attribute sequencing to each sample.
attributes.tag: ENA first public
attributes.value: 2021-01-12
attributes.tag: ENA last update
attributes.value: 2021-01-08
attributes.tag: External Id
attributes.value: SAMEA7773326
attributes.tag: INSDC center alias
attributes.value: FUDAN UNIVERSITY
attributes.tag: INSDC center name
attributes.value: FUDAN UNIVERSITY
attributes.tag: INSDC first public
attributes.value: 2021-01-12T05:00:13Z
attributes.tag: INSDC last update
attributes.value: 2021-01-08T11:03:56Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-9865_2:K_2
attributes.tag: age
attributes.value: 12
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: intestinal epithelial cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: Bach2-/-
attributes.tag: organism part
attributes.value: epithelium of small intestine
attributes.tag: sample name
attributes.value: E-MTAB-9865_2:K_2
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: C57BL/6J