organism: Mus musculus
description: Protocols: Embryonic mammary tissues (embryonic mammary primordial epithelium (MPE), embryonic mammary stroma (includes mammary mesenchyme (MM) and fat pad precursor (FPP)), and surface epithelium (Epi), were micro-dissected from E12.5 B6 mice following the protocol described in Sun L, Lee MY, & Veltmaat JM (2011) A non-enzymatic microsurgical dissection technique of mouse embryonic tissues for gene expression profiling applications. Int J Dev Biol 55(10-12):969-974. Total RNA from mammary tissues were extracted using RNeasy Mini kit 74104, Qiagen, Manchester, UK), Amplified cDNA from embryonic mammary tissue was generated by the SMARTer amplification kit. The cDNA was end-repaired, A-tailed, adapter-ligated and amplified. The prepared libraries were size selected, multiplexed and quality checked.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-11-30T11:02:52Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-06-06T16:55:45Z
attributes.tag: External Id
attributes.value: SAMEA4712217
attributes.tag: INSDC center name
attributes.value: The Institute of Cancer Research
attributes.tag: INSDC first public
attributes.value: 2018-11-30T11:02:52Z
attributes.tag: INSDC last update
attributes.value: 2018-06-06T16:55:45Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6859:mammary mesenchyme rep2.1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: mesodermal cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: embryonic day 12.5
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: mammary bud
attributes.tag: sample name
attributes.value: E-MTAB-6859:mammary mesenchyme rep2.1
attributes.tag: sampling site
attributes.value: embryonic mammary stroma
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: C57BL/6