organism: Mus musculus
description: Protocols: Mice were humanely euthanized and the brain was removed. A coronal section containing the hypothalamus was removed from the brain using an adult mouse brain matrix. The hypothalamus was dissected from this section and immediately flash-frozen in liquid nitrogen until all samples had been collected. see sample collection protocol not applicable Wild-type, heterozygote and homozygote littermates were fed a 55% high-fat diet (Research Diets) starting at 6 weeks of age and concluding at 16 weeks of age at which time animal were humanely sacrificed for RNA profiling. All three genotypes were present in each cage. Complementary DNA was synthesized from 1 ug of RNA using Superscript II Reverse Transcriptase (Thermo Fisher 18064014) with oligo(dT). Frozen tissue was resuspended in 300 ul TriReagent on ice and then homozenized. Total RNA was isolated with the Zymo Direct-zol RNA purification kit (R2060) following manufacturer's instructions. RNA-seq libraries were generated with the Illumina TruSeq V2 kit (Illumina, RS-122-2001) and 500 ng of RNA, following manufacturer's instructions.
attributes.tag: ENA first public
attributes.value: 2020-01-03
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-02-27
attributes.tag: External Id
attributes.value: SAMEA5339772
attributes.tag: INSDC center alias
attributes.value: UNIVERSITY OF CHICAGO
attributes.tag: INSDC center name
attributes.value: UNIVERSITY OF CHICAGO
attributes.tag: INSDC first public
attributes.value: 2020-01-03T15:59:17Z
attributes.tag: INSDC last update
attributes.value: 2023-02-27T14:47:59Z
attributes.tag: INSDC status
attributes.value: suppressed
attributes.tag: Submitter Id
attributes.value: E-MTAB-7691:wt-1
attributes.tag: age
attributes.value: 16
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: individual
attributes.value: 1
attributes.tag: organism part
attributes.value: hypothalamus
attributes.tag: sample name
attributes.value: E-MTAB-7691:wt-1
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: C57/B6
attributes.tag: tag number
attributes.value: 450