organism: Aphidius ervi
description: Adult parasitoids (males and females) were collected live and stored in 1.5 ml centrifuge tubes containing ethanol 95% at -20°C until DNA extraction
attributes.tag: strain
attributes.value: Acyrthosiphon Pisum (AP)
attributes.tag: age
attributes.value: adult
attributes.tag: dev_stage
attributes.value: adult
attributes.tag: sex
attributes.value: pooled male and female
attributes.tag: tissue
attributes.value: whole body
attributes.tag: biomaterial_provider
attributes.value: Universidad de Talca, Talca, Chile
attributes.tag: breeding_history
attributes.value: Aphidius ervi parasitoids were reared in fresh host plants containing wasp-naïve aphids from the same aphid host from which they emerged under controlled conditions [16 h light: 8 h dark, 20°C]. Pea aphids were reared on broad bean (Vicia faba L.). These lineages were propagated for approximately 75 generations under controlled conditions . Each week, fresh host plants containing wasp-naïve aphids were introduced into the A. ervi rearing cages for parasitoid population maintenance, together with vials containing diluted honey and water for adult parasitoid feeding. Further reduction of intra-population genetic differences was accomplished by establishing isofemale parasitoid lines. These isofemale lines were maintained as described previously and propagated for approximately 10 generations before wasps' collection for genomic sequencing.
attributes.tag: collected_by
attributes.value: Gabriel Ballesteros
attributes.tag: isolation_source
attributes.value: These lineages were first collected as parasitized aphids (recognizable as mummies) from fields of cereals and legumes in two different geographic zones in Chile: Region de Los Rios and Region del Maule. S. avenae aphids were sampled on wheat (Triticum aestivum L.) .
attributes.tag: BioSampleModel
attributes.value: Model organism or animal