organism: Mus musculus
description: Protocols: Foxp3-GFP KI mice were infected with 200 pfu of the Lymphocytic choriomeningitis virus (strain WE) (LCMV WE strain) or left uninfected (naive). Infection with low dose LCMV WE induces an acute systemic Th1-driven immune response which is accompanied by a peak in CD4+Foxp3+CXCR3+ expressing Treg cells at day 14 post infection. At day 14 post infection spleens and peripheral LNs were collected, mashed through a metal grid or a 70 µm cell strainer, respectively to obtain a single cell suspension. Red blood cells were lysed via ACK lysis. CD4+ T cells were MACS sorted using anti-CD4 beads (Miltenyi) prior to flow sorting of CD4+Foxp3-GFP+ Treg cells, CD4+Foxp3-GFP- Tconv from naive mice or CD4+Foxp3-GFP+CXCR3+ Treg cells, CD4+Foxp3-GFP+ Treg cells or CD4+Foxp3-GFP- Tconv from LCMV-infected mice. RNA was extracted using the Qiagen RNeasy Micro Kit. Libraries for sequencing were generated with the TruSeq RNA Sample Preparation kit (Illumina).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-03-11T04:05:41Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-03-06T16:25:39Z
attributes.tag: External Id
attributes.value: SAMEA6613570
attributes.tag: INSDC center name
attributes.value: University of Zurich
attributes.tag: INSDC first public
attributes.value: 2020-03-11T04:05:41Z
attributes.tag: INSDC last update
attributes.value: 2020-03-06T16:25:39Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8861:Sample 15
attributes.tag: age
attributes.value: 4 to 23
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: regulatory T cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: LCMW WE
attributes.tag: genotype
attributes.value: Foxp3-GFP KI
attributes.tag: organism part
attributes.value: spleen and lymph node
attributes.tag: phenotype
attributes.value: CXCR3+CD4+Foxp3+
attributes.tag: sample name
attributes.value: E-MTAB-8861:Sample 15
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: C57BL/6