organism: Homo sapiens
description: Protocols: MCF7-ELF5-Isoform2-V5 cells treated with Dox or vehicle from 24 hours after plating for 48 hours. RNA was extracted with phenol/chloroform using the miRNeasy Mini Kit (Qiagen) with on-column DNase treatment. RNA quality was assessed on the 2100 Bioanalyser using RNA Nano chips (Agilent), with all samples having an RNA Integrity Number (RIN) of 9.8-9.9 (out of 10). Samples were prepared with the TruSeq Stranded Total RNA Sample prep kit (RS-122-2201 Illumina, San Diego, California, USA) according to the manufacturer's instructions. One ug of total RNA was used as input to the ribosomal ribozero RNA depletion, followed by 13 cycles of PCR to amplify the adapter-ligated cDNA.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-01-06T04:04:24Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-01-31T16:19:11Z
attributes.tag: External Id
attributes.value: SAMEA5313174
attributes.tag: INSDC center name
attributes.value: Garvan Institute of Medical Research and The Kinghorn Cancer Centre
attributes.tag: INSDC first public
attributes.value: 2020-01-06T04:04:24Z
attributes.tag: INSDC last update
attributes.value: 2019-01-31T16:19:11Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7641:RNA-seq Dox1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: MCF-7
attributes.tag: cell type
attributes.value: BTO:0000093
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: Cancer cell line
attributes.tag: disease
attributes.value: breast carcinoma
attributes.tag: genotype
attributes.value: MCF7-ELF5-Isoform2-V5
attributes.tag: organism part
attributes.value: breast
attributes.tag: replicate
attributes.value: 1
attributes.tag: sample name
attributes.value: E-MTAB-7641:RNA-seq Dox1
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: stimulus
attributes.value: Doxycyclin