organism: Candida albicans
description: Protocols: Cell harvesting Initial Growth: Cells were cultured in YPD at 30 °C for 16.5 hours to an OD600 = ~0.8 before collection by (2 min. at 1000 x g at 4 ◦C) and wash by distilled water. Treatment: cells were resuspended in 15 ml pre-warmed final media (YPD or RPMI) and grown at an appropriate temperature (30 ◦C or 37 ◦C respectively) for 90 minutes. OD600 values were recorded (OD600 = ~1.5) followed by RNA extraction. RNA extraction with modifications: Omega EZNA Yeast RNA Kit (VWR) following the kit protocol with two modifications: (1) 30 °C incubation in SE Buffer/2-mercaptoethanol/lyticase solution time was increased to 90 minutes (2) lysis was performed with bead mill at top speed for 30 minutes at 4 °C. DNase I digestion was included prior to final wash steps. RNA was eluted with 50 µL DEPC water (RNase inactivated) into low binding tubes. In addition to absorbance readings (260/280 ~2), quality was analysed by formaldehyde gel electrophoresis. Illumina
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-12-01T17:02:12Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-06-19T11:03:24Z
attributes.tag: External Id
attributes.value: SAMEA4743750
attributes.tag: INSDC center name
attributes.value: University of Kent
attributes.tag: INSDC first public
attributes.value: 2018-12-01T17:02:12Z
attributes.tag: INSDC last update
attributes.value: 2018-06-19T11:03:24Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6920:460-3R
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: genotype
attributes.value: MTL a/alpha ura3-iro1::imm434/URA3-IRO1 his1/his1 arg4/arg4 leu2/leu2 hda3/hda3
attributes.tag: growth condition
attributes.value: RPMI; 37 degree celsius
attributes.tag: sample name
attributes.value: E-MTAB-6920:460-3R
attributes.tag: scientific_name
attributes.value: Candida albicans
attributes.tag: strain
attributes.value: hda3/ (SN152 parental strain)