organism: Homo sapiens
description: Protocols: Cells are seed in the plate until 80% confluence. Then cells were washed by PBS twice, and lysed with the lysis buffer. Cells were maintained in Minimum Essential Medium (MEM) with10% FBS and penicillin-streptomycin at 37°C with 5% CO2 and 5% O2 and over 95% humidify. SOX2 and SFRP2 transduction in glioblastoma cells were performed using lentiviral transfection systme. Cells were selected by using puromycin. Total RNA was purified using the PureLink® kit following the manufacturer's instructions (Thermo Fisher Scientific) Illumina TruSeq strand-specific RNA libraries (poly-A selection)
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-01-03T15:59:16Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-01-15T08:54:25Z
attributes.tag: External Id
attributes.value: SAMEA5225178
attributes.tag: INSDC center name
attributes.value: Department of Oncology-pathology, Karolinska Institutet
attributes.tag: INSDC first public
attributes.value: 2020-01-03T15:59:16Z
attributes.tag: INSDC last update
attributes.value: 2019-01-15T08:54:25Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7591:U-2987-control (YFP)-1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: U-2987
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: glioblastoma
attributes.tag: genotype
attributes.value: pLEX-blast-V5-YFP
attributes.tag: organism part
attributes.value: brain
attributes.tag: phenotype
attributes.value: wild type
attributes.tag: sample name
attributes.value: E-MTAB-7591:U-2987-control (YFP)-1
attributes.tag: scientific_name
attributes.value: Homo sapiens