organism: Homo sapiens
description: Protocols: We generated RNH1 knock out K562 cells using the CRISPR/Cas9 method. Total RNA was isolated from wild type and RNH1 deficient K562 cells. Illumina TruSeq Stranded mRNA protocol Illumina TruSeq Stranded mRNA protocol. We prepared Illumina TruSeq stranded mRNA libraries. The libraries were subjected to a preparative size selection on a PippinHT instrument to exclude library molecules with insert sizes of less than 300 bp.
attributes.tag: ENA checklist
attributes.value: ERC000011
attributes.tag: INSDC center name
attributes.value: Department of Clinical Research, Bern University, Switzerland
attributes.tag: INSDC first public
attributes.value: 2017-03-05T17:02:09Z
attributes.tag: INSDC last update
attributes.value: 2017-02-23T14:30:41Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: SRA accession
attributes.value: ERS1571145
attributes.tag: Sample Name
attributes.value: ERS1571145
attributes.tag: alias
attributes.value: E-MTAB-5162:K562WT1_S69
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: K562
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism
attributes.value: Homo sapiens
attributes.tag: title
attributes.value: K562WT1_S69