organism: Mus musculus
description: Protocols: Myotubes were grown in adherent condition, they are fixed in Formaldehyde 1% solution, quenched in Glycine, washed in PBS and then collected with scrapers. Cells are opportunely lised and the chromatin is then sonicated to final size of about 300bp. Chromatin was immunoprecipitated with the described antibody Confluent myoblasts are differentiated in DMEM containing 2% Horse Serum, at 37°C in 5% of CO2 following standard protocols Myotubes day 1 were treated with 100 uM of H2O2 for 24 hours Illumina prep library
attributes.tag: ENA first public
attributes.value: 2017-03-01
attributes.tag: ENA last update
attributes.value: 2016-06-16
attributes.tag: External Id
attributes.value: SAMEA4036476
attributes.tag: INSDC center alias
attributes.value: INGM Istituto Nazionale di Genetica Molecolare \Romeo ed Enrica Invernizzi\
attributes.tag: INSDC center name
attributes.value: INGM Istituto Nazionale di Genetica Molecolare \Romeo ed Enrica Invernizzi\
attributes.tag: INSDC first public
attributes.value: 2017-03-01T17:07:12Z
attributes.tag: INSDC last update
attributes.value: 2016-06-16T22:02:52Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-4794:Input_H2O2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: C2C12
attributes.tag: common name
attributes.value: house mouse
attributes.tag: sample name
attributes.value: E-MTAB-4794:Input_H2O2
attributes.tag: stimulus
attributes.value: Hydrogen peroxide