organism: Homo sapiens
description: Protocols: Under signed informed consent, peripheral blood from healthy volunteers was obtained for the generation of antigen-specific T cells. Genomic DNA was isolated using a QIAamp DNA Mini Kit (Qiagen) and quantified with Qubit DNA Br kit according to manufactures' instructions. Genomic DNA was used for amplicon specific PCR using genome specific primers which flanked the expected on-target site for GR CRISPR/Cas9 target, and 7 (2 exonic and 5 intronic) in silico predicted potential offsite targets (named Off-site1-7). The 8 PCR products from each sample were pooled and 100ng of the final pool were used as input for the library construction with the NEBNext Ultra II Library Prep kit (New England Biolabs, #E7645). Library quantification was performed with the KAPA SYBR FAST Universal qPCR kit (KAPA Biosystems, #4824).
attributes.tag: ENA first public
attributes.value: 2021-01-14
attributes.tag: ENA last update
attributes.value: 2020-12-22
attributes.tag: External Id
attributes.value: SAMEA7746876
attributes.tag: INSDC center alias
attributes.value: Institute of Applied Biosciences (INAB), Centre for Research and Technology Hellas (CERTH), Thessaloniki, Greece
attributes.tag: INSDC center name
attributes.value: Institute of Applied Biosciences (INAB), Centre for Research and Technology Hellas (CERTH), Thessaloniki, Greece
attributes.tag: INSDC first public
attributes.value: 2021-01-14T04:14:27Z
attributes.tag: INSDC last update
attributes.value: 2020-12-22T15:59:49Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-9968:Cb-STs_AK
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: pathogen-specific T-cells targeting CMV, EBV, BKV, adenovirus and Aspergillus fumigatus
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: normal
attributes.tag: genetic modification
attributes.value: CRISPR/Cas9-mediated disruption of the glucocorticoid receptor
attributes.tag: organism part
attributes.value: blood
attributes.tag: sample name
attributes.value: E-MTAB-9968:Cb-STs_AK