organism: Solea senegalensis
description: Protocols: N=6 pseudo-albino or n=6 normally pigmented juvenile sole (6-month old) belonging to the same spawning batch in Aquaculture (Cadiz, Spain) were anesthetized and dorsal skin and anterior gut samples were excised and incubated in RNA-later at 4ºC for 24 h and stored at -20ºC until RNA extraction. Total RNA was extracted using an E.Z.N.A. Total RNA Kit I (Omega-Biotek) following mechanical disruption of skin and gut tissue fragments using 5 mm iron beads (Qiagen) for 2x20sec at 6,800 rpm in a Precellys 24. RNA samples were treated with DNAse to remove possible DNA contamination. Four RNA-seq libraries were constructed using pools of total RNA from dorsal skin and anterior gut of pseudo-albino or pigmented sole (n=4/pool, 250 ng/individual). RNA-seq libraries were prepared using an Illumina TruSeq RNA sample preparation kit and TruSeq index adaptors.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-01-03T15:59:15Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-11-26T13:00:38Z
attributes.tag: External Id
attributes.value: SAMEA5130945
attributes.tag: INSDC center name
attributes.value: Centre of Marine Sciences (CCMAR)
attributes.tag: INSDC first public
attributes.value: 2020-01-03T15:59:15Z
attributes.tag: INSDC last update
attributes.value: 2018-11-26T13:00:38Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7434:Pseudo-albino gut
attributes.tag: age
attributes.value: 6
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: Senegalese sole
attributes.tag: developmental stage
attributes.value: juvenile stage
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: individual
attributes.value: mixed pool of 4 fish
attributes.tag: organism part
attributes.value: intestine
attributes.tag: phenotype
attributes.value: pseudo-albino
attributes.tag: sample name
attributes.value: E-MTAB-7434:Pseudo-albino gut
attributes.tag: scientific_name
attributes.value: Solea senegalensis
attributes.tag: sex
attributes.value: mixed