organism: Mus musculus
description: Protocols: Primary fibroblasts were derived from adult CAST/EiJ x C57/Bl6 mice. Tail explants were cultured in fibroblast medium (DMEM high glucose, 10% ES cell FBS, 1% Penicillin/Streptomycin, 1% non-essential amino acids, 1% sodium-puryvate, 0.1mM b-Mercaptoethanol). Cells were passaged twice before distributed as individual cells into wells of 384 or 96 well plates using a FACS. Wells was pre-loaded with lysis buffer. ES cells were cultured as in Meissner et al. Methods Mol Biol 2009, and similarly distributed via FACS into as single cells into wells of 96 and 384-well plates Cell lysis. Smart-seq2, indexed by Nextera i5 and i7 and with a unique molecular identifier present in template-switching oligonucleotide. No exogenous spike-ins were used..
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-11-30T11:01:58Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-07-30T11:05:01Z
attributes.tag: External Id
attributes.value: SAMEA4808699
attributes.tag: INSDC center name
attributes.value: Karolinska Institutet, Department of Cell and Molecular Biology
attributes.tag: INSDC first public
attributes.value: 2018-11-30T11:01:58Z
attributes.tag: INSDC last update
attributes.value: 2018-07-30T11:05:01Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7098:Sample 252
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: fibroblast
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: dermis
attributes.tag: sample name
attributes.value: E-MTAB-7098:Sample 252
attributes.tag: sampling site
attributes.value: tail
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: C57BL/6 x CAST/EiJ