organism: Homo sapiens
description: Protocols: hPSCs were differentiated into endothelial cells capable of haemogenic potential following a small molecules-driven differentiation protocol. These cells were detached from their plates in culture using TryplE and resuspended in culture media. Cells were differentiated until the emergence of haematopoietic clusters at EHT D3, when the first samples were collected and processed for scRNAseq (CTRL1). Alternatively, they were further cultured for 48 hours applying one of the following treatments: - 0.1% DMSO (CTRL2) - 1 μM PD0332991 - 10 μM RO3306 - 4 μM Roscovitine - 2 μM GSK126 At the end of the treatment, cells were collected and processed for scRNAseq. Conversion was performed following the 10X protocol (https://www.10xgenomics.com/) Chromium 10X platform. Constructed by core facility at Wellcome Sanger Institute.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-05-17T17:05:10Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-05-16T21:27:52Z
attributes.tag: External Id
attributes.value: SAMEA6844878
attributes.tag: INSDC center name
attributes.value: Wellcome Sanger Institute
attributes.tag: INSDC first public
attributes.value: 2020-05-17T17:05:10Z
attributes.tag: INSDC last update
attributes.value: 2020-05-16T21:27:52Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-82051589660429:OT10xRNA8764427
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: A1AT-RR
attributes.tag: cell type
attributes.value: haematopoietic stem cell
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: Alpha-1-antitrypsin deficiency
attributes.tag: genotype
attributes.value: corrected Glu342Lys mutation
attributes.tag: organism part
attributes.value: skin
attributes.tag: progenitor cell type
attributes.value: induced pluripotent stem cell
attributes.tag: sample name
attributes.value: E-MTAB-82051589660429:OT10xRNA8764427
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: male