organism: Mus musculus
description: The FUT2 gene encodes an α-1,2-fucosyltransferase responsible for the expression of ABO histo-blood-group antigens on mucosal surfaces and bodily secretions. Individuals who carry at least one functional allele are known as “secretors”, whereas those homozygous for loss-of- function mutations are known as “nonsecretors”. Nonsecretor individuals are more susceptible to chronic inflammatory disorders such as Crohn Disease, which may be mediated by alterations in the microbiome. Here, we investigated the dynamics of microbial community assembly with respect to genotype using a Fut2-deficient mouse model, taking the genotype of the maternal lineage over two generations into account. We found strong differences in community assembly of microbial communities over time, depending on the Fut2 genotype of the host and that of their progenitors. By applying interaction network analyses, we further identified patterns of specialization and stabilization over time, which are influenced by the host and parental genotype during the process of community development. We also show genotype- and breeding-dependent patterns of community susceptibility to disturbance in a novel in silico approach integrating ecological- and network analysis. Our results indicate that it may be important to investigate the influence of Fut2 genotype in a familial context in order to fully understand its role in the etiology of chronic inflammatory disorders.
attributes.tag: ENA first public
attributes.value: 2017-02-27
attributes.tag: ENA last update
attributes.value: 2016-10-21
attributes.tag: External Id
attributes.value: SAMEA4066412
attributes.tag: INSDC center alias
attributes.value: MPIEVB
attributes.tag: INSDC center name
attributes.value: Max-Planck-Institute for Evolutionary Biology
attributes.tag: INSDC first public
attributes.value: 2017-02-27T17:01:46Z
attributes.tag: INSDC last update
attributes.value: 2016-10-21T09:40:21Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: 226_11_colon
attributes.tag: adapters
attributes.value: CGTATCGCCTCCCTCGCGCCATCAG
attributes.tag: breeding cage
attributes.value: ZK9A(m_wt)
attributes.tag: breeding direction
attributes.value: female Fut2-/-
attributes.tag: cage
attributes.value: 58
attributes.tag: collection date
attributes.value: 2012-08-04
attributes.tag: common name
attributes.value: house mouse
attributes.tag: environment (biome)
attributes.value: intestine environment
attributes.tag: environment (feature)
attributes.value: microbial feature
attributes.tag: environment (material)
attributes.value: organic material
attributes.tag: geographic location (country and/or sea)
attributes.value: Germany
attributes.tag: geographic location (latitude)
attributes.value: 54.338410
attributes.tag: geographic location (longitude)
attributes.value: 10.120036
attributes.tag: geographic location (region and locality)
attributes.value: Schleswig-Holstein, Kiel
attributes.tag: host age
attributes.value: 15
attributes.tag: host body habitat
attributes.value: gut
attributes.tag: host body product
attributes.value: tissue,mucosa
attributes.tag: host body site
attributes.value: colon
attributes.tag: host common name
attributes.value: house mouse
attributes.tag: host disease status
attributes.value: healthy
attributes.tag: host genotype
attributes.value: Fut2+/-
attributes.tag: host infra specific name
attributes.value: C57BL6/J
attributes.tag: host infra specific rank
attributes.value: strain
attributes.tag: host life stage
attributes.value: adult
attributes.tag: host sex
attributes.value: male
attributes.tag: host subject id
attributes.value: 226
attributes.tag: host taxid
attributes.value: 10090
attributes.tag: host-associated environmental package
attributes.value: host-associated
attributes.tag: investigation type
attributes.value: metagenome
attributes.tag: multiplex identifiers
attributes.value: CAGTAGACGTCA
attributes.tag: nucleic acid amplification
attributes.value: PMCID:3223430
attributes.tag: nucleic acid extraction
attributes.value: DNA Stool Kit (Qiagen) following manufacturer's protocol including a bead-beating step
attributes.tag: pcr conditions
attributes.value: 30s-98°C;30x9s-98°C,30s-55°C,30s-72°C;10min-72°C
attributes.tag: pcr primers
attributes.value: F-AGAGTTTGATCCTGGCTCAG,R-TGCTGCCTCCCGTAGGA
attributes.tag: project name
attributes.value: Fut2 metagenome
attributes.tag: sample material processing
attributes.value: material processing,denaturing,freezing
attributes.tag: sample name
attributes.value: 226_11_colon
attributes.tag: sequencing method
attributes.value: Illumina
attributes.tag: source material identifiers
attributes.value: bio_material
attributes.tag: target gene
attributes.value: 16S rRNA
attributes.tag: target subfragment
attributes.value: V1-V2