organism: Mus musculus
description: Protocols: Jejunum and ileum were flushed with ice-cold PBS and enterocytes were extracted by shaking them in tissue lysis buffer (HBSS, 5 Mm EDTA and 250 µM DTT) for 30 minutes at 37oC. Mice were kept under specific pathogen-free conditions at the Hadassah Medical School of the Hebrew University. All mouse experiments were performed in accordance with guidelines of the relevant institution's ethics committee. CKIα and p53 KO was induced by 4 subcutaneous tamoxifen injections (150 mg/kg) every alternate day, followed by mice sacrifice. CKIα and p53 KO was induced by 4 subcutaneous tamoxifen injections (150 mg/kg) every alternate day, followed by mice sacrifice. Mice were treated with gallic acid in the drinking water for 4 weeks prior KO induction + 1 week during the tamoxifen (KO) injections. Total RNA was extracted from cell pellets using miRNeasy extraction kit (QIAGEN), according to manufacturer's instructions. For quality control of RNA extraction yield and Library synthesis products, RNA ScreenTape kit (Agilent technologies), D1000 ScreenTape kit (Agilent technologies), Qubit® RNA HS Assay kit (Invitrogen), Qubit® DNA HS Assay kit (Invitrogen) were used for each specific step purpose. KAPA Stranded mRNA-seq kit with mRNA capture beads (Kapa Biosystems) was used. In brief, 1 µg was used for the library construction, library was eluted in 20 µl of elution buffer. Libraries were adjusted to 10 mM, then 10 µl from each sample was collected and pooled in one tube.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-01-03T15:59:19Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-04-06T10:54:36Z
attributes.tag: External Id
attributes.value: SAMEA5554956
attributes.tag: INSDC center name
attributes.value: Institute of Molecular Oncology, Center for Tumor and Immunobiology, Philipps-University Marburg
attributes.tag: INSDC first public
attributes.value: 2020-01-03T15:59:19Z
attributes.tag: INSDC last update
attributes.value: 2019-04-06T10:54:36Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7859:4a
attributes.tag: age
attributes.value: 3.5
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: enterocyte
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: CKIgut/p53R172H
attributes.tag: individual
attributes.value: mouse 10
attributes.tag: organism part
attributes.value: ileum
attributes.tag: sample name
attributes.value: E-MTAB-7859:4a
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: C57BL/6