organism: Sus scrofa
description: Protocols: The experiment procedures took place at approximately the same time (10:00 to 12:00 AM). Three hours after the treatment of dexamethasone, the animals were anesthetized by an intravenous (i.v.) administration of a combination of ketamine (Ursotamin; Serum-Werk Bernburg, Bernburg, Germany) and azaperone (Stresnil; Janssen-Cilag, Neuss, Germany), and euthanized by an i.v. administration of T61 (Intervet, Unterschleißheim, Germany). Tissue samples were quickly dissected (<10min), frozen in liquid nitrogen, and stored at -80ºC. The experimental protocol (7221.3-1-024/16) has been approved by the Animal Care Committee of the Leibniz Institute for Farm Animal Biology and the State Mecklenburg-Western Pomerania (Landesamt für Landwirtschaft, Lebensmittelsicherheit und Fischerei). 11 or 22 are genotypes of the pigs at the NR3C1 c.1829C>T (rs335303636, SNP) which 11 stands for homozygous CC and 22 stands for TT (Reyer et a. 2016; PMID 27736993) Total RNA was isolated using TRI reagent (Sigma-Aldrich, Taufkirchen, Germany). DNA traces were removed by DNaseI-treatment (Baseline-Zero DNase; Biozym, Hessisch Oldendorf, Germany), and the RNA was cleaned-up using the RNA Clean&Concentrator-25 Kit (Zymo Research, Freiburg, Germany). RNA quantity was determined using a Qubit fluorometer (ThermoFisher Scientific, Germany), and integrity was checked on an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). 2 µg of total RNA with RIN > 8 was used for library preparation using a TruSeq Stranded mRNA Kit with 11 cycles of PCR-amplification according to the manufacture's recommendation (Illumina).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-01-03T15:59:15Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-12-07T13:56:46Z
attributes.tag: External Id
attributes.value: SAMEA5158129
attributes.tag: INSDC center name
attributes.value: Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit
attributes.tag: INSDC first public
attributes.value: 2020-01-03T15:59:15Z
attributes.tag: INSDC last update
attributes.value: 2018-12-07T13:56:46Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7494:H6925
attributes.tag: age
attributes.value: 7
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: pig
attributes.tag: developmental stage
attributes.value: piglet
attributes.tag: genotype
attributes.value: homozygous SNP NR3C1 c.1829CC
attributes.tag: individual
attributes.value: 6925
attributes.tag: organism part
attributes.value: hypothalamus
attributes.tag: sample name
attributes.value: E-MTAB-7494:H6925
attributes.tag: scientific_name
attributes.value: Sus scrofa
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: German Landrace