organism: Homo sapiens
description: Protocols: Cell culture dishes were harvested. monocytes differentiated into MDMs, cultured in R5 media (RPMI + 5 % human AB Serum + 1 % Sodium Pyruvate. Media was changed 2 hours before treatment. Treated for 3 hours with the specified compound or solvent (EtOH) control. NucleoSpin RNA Kit (Macherey-Nagel, 740955.250); DNA-free™ DNA Removal Kit (ThermoFisher/Ambion, AM1906); according to manufacturer's protocol Libraries were created with the Lexogen QuantSeq 3′ mRNA-Seq Library Prep Kit FWD for Illumina.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-12-10T04:04:51Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-02-26T14:15:55Z
attributes.tag: External Id
attributes.value: SAMEA6591607
attributes.tag: INSDC center name
attributes.value: Phillips University Marburg
attributes.tag: INSDC first public
attributes.value: 2020-12-10T04:04:51Z
attributes.tag: INSDC last update
attributes.value: 2020-02-26T14:15:55Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8830:MD001
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: macrophage
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: blood
attributes.tag: progenitor cell type
attributes.value: peripheral blood mononuclear cell
attributes.tag: sample name
attributes.value: E-MTAB-8830:MD001
attributes.tag: scientific_name
attributes.value: Homo sapiens