organism: Homo sapiens
description: Protocols: We isolated PBMCs and sorted monocytes and CD4 T cells through FACS prior to the initiation of biological therapy. All recruited patients had endoscopy proven disease at time of sample collection. Total RNA lysed, sorted CD4+ and CD14+ cells was extracted using the AllPrep DNA/RNA Mini kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions. The integrity and quantity of all RNA was assessed with a 2100 Bioanalyzer (Agilent, Waldbronn, Germany) and a Nanodrop ND-1000 spectrophotometer (Thermo Scientific, Waltham, Massachusetts, USA). Library preparation was performed using the TruSeq Stranded mRNA protocol (Illumina, San Diego, USA) according to the manufacturer's instructions.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-12-30T04:03:54Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-05-02T09:10:19Z
attributes.tag: External Id
attributes.value: SAMEA5587570
attributes.tag: INSDC center name
attributes.value: Dpt. Gastroenterology and Hepatology University Hospitals Leuven
attributes.tag: INSDC first public
attributes.value: 2019-12-30T04:03:54Z
attributes.tag: INSDC last update
attributes.value: 2019-05-02T09:10:19Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7927:GC061549
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: CD4 T cell
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: Crohn's disease
attributes.tag: individual
attributes.value: Patient11
attributes.tag: organism part
attributes.value: blood
attributes.tag: sample name
attributes.value: E-MTAB-7927:GC061549
attributes.tag: scientific_name
attributes.value: Homo sapiens