organism: Mus musculus
description: Protocols: The preparation of the material was as described in Kutejova et al., 2008. First branchial arches were dissected (the whole tissue), crosslinked and frozen in order to pool enough for ChIP. Kutejova E, Engist B, Self M, Oliver G, Kirilenko P, Bobola N. Six2 functions redundantly immediately downstream of Hoxa2. Development. 2008. 135(8):1463-70. PMID: 18321982. MinElute PCR purification kit (Qiagen) ChIP-seq was performed as described in Donaldson et al., 2012, using 20 ug of H3K27Ac antibody (Abcam, ab4729). Donaldson I, Amin S, Hensman J, Kutejova E, Rattray M, Lawrence N, Hayes A, Ward C and Bobola N. Genome-wide occupancy links Hoxa2 to Wnt-beta-catenin signaling in mouse embryonic development. Nucleic Acids Res. 2012. 40(9):3990-4001. 70 to 100 arches were used for each sample. Library preparation was performed using the TruSeq ChIP Sample Preparation kit (Illumina).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-01-06T17:04:14Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-05-20T14:04:57Z
attributes.tag: External Id
attributes.value: SAMEA5618889
attributes.tag: INSDC center name
attributes.value: UNIVERSITY OF MANCHESTER
attributes.tag: INSDC first public
attributes.value: 2020-01-06T17:04:14Z
attributes.tag: INSDC last update
attributes.value: 2019-05-20T14:04:57Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7966:H3K27Ac_BA1_Input_rep2
attributes.tag: age
attributes.value: embryonic day 11.5
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: embryo
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: individual
attributes.value: pool of embryos
attributes.tag: organism part
attributes.value: pharyngeal arch 1
attributes.tag: sample name
attributes.value: E-MTAB-7966:H3K27Ac_BA1_Input_rep2
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: mixed sex
attributes.tag: strain
attributes.value: CD1