organism: Drosophila melanogaster
attributes.tag: strain
attributes.value: not applicable
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: breed
attributes.value: not applicable
attributes.tag: cultivar
attributes.value: not applicable
attributes.tag: ecotype
attributes.value: not applicable
attributes.tag: age
attributes.value: not applicable
attributes.tag: dev_stage
attributes.value: adult
attributes.tag: sex
attributes.value: pooled male and female
attributes.tag: tissue
attributes.value: head
attributes.tag: biomaterial_provider
attributes.value: Hoffmann Lab, School of BioSciences, University of Melbourne, 30 Flemington Rd, Parkville 3010 Victoria, Australia
attributes.tag: breeding_history
attributes.value: The offspring of 60 field-collected females were pooled and mass-bred for two generations in the laboratory to a size of approx. 5000 individuals (generation 0). These 5000 virgin females and males were separated by sex under light CO2 anesthesia and held in separate vials according to sex, at a density of 25 individuals per vial. A random subset of flies were randomly allocated into five replicate lines comprising 100Ñ110 flies of each sex (200Ñ210 in total) per replicate. For each of the following generations, 1000 females and 1000 males per line were retained and a randomly-chosen 10% of adults (100Ñ110 of each sex, 200Ñ210 in total) were kept as parents for the next generation. Population sizes were maintained in this way every generation until generation eight, and every second generation thereafter until generation 21. In the alternate generations, populations were maintained at approx. 2000 individuals. This BioSample is a pool of 25 females and 25 males from Replicate 4 after the 2+21 generations of culture (the same timepoint as the selected lines after 13 generations of selection).
attributes.tag: breeding_method
attributes.value: All cultures were held at constant 19¡C, under 12 : 12 h light : dark cycle in 250 ml bottles containing laboratory medium composed of dextrose (7.5% w/v), cornmeal (7.3% w/v), inactive yeast (3.5% w/v), soy flour (2% w/v), agar (0.6% w/v), 4-methyl 4-hydroxybenzoate (1.6%) and acid mix (1.4% 10:1 proprionic acid:orthophosphoric acid). The experimental flies were reared under controlled density conditions by removing parents from the bottles after 48 h of oviposition.
attributes.tag: collected_by
attributes.value: Sandra Hangartner, sangartner@hotmail.com
attributes.tag: geo_loc_name
attributes.value: Australia: Templestowe, Melbourne, Victoria
attributes.tag: sample_type
attributes.value: pool of 25 male and 25 female heads (per experiment)
attributes.tag: BioSampleModel
attributes.value: Model organism or animal