organism: seawater metagenome
description: Seawater-associated microbial community sampled from sablefish rearing tanks. 1200 mL of seawater was collected onto 0.22 µm filters and DNA was extracted using the Qiagen Dneasy PowerWater Sterivex kit and used for PCR amplification of 16S rRNA gene (V3-4) with primers 341F/806R. PCR products were pair-end sequenced (2 x 300 bp) on an Illumina MiSeq.
attributes.tag: host
attributes.value: not applicable
attributes.tag: isolation_source
attributes.value: Puget Sound, Washington, USA
attributes.tag: collection_date
attributes.value: 2019-06-03
attributes.tag: geo_loc_name
attributes.value: USA: NOAA research laboratory, Machester, Washington
attributes.tag: lat_lon
attributes.value: 47.571199 N 122.552665 W
attributes.tag: source_material_id
attributes.value: 6_3_19_Taurine_Tank4_Seawater_4h_16S
attributes.tag: BioSampleModel
attributes.value: Metagenome or environmental