organism: Mus musculus
description: Protocols: C57BL/6J mice were purchased at 8 weeks old, acclimatized for one week, and sham-operated at 9 weeks old. After 45 days of recovery at basal laboratory conditions (chow at libitum, 22C), mice were fasted for 4 hours in the morning. Subsequently, mice were euthanized by cardiac puncture under isoflurane anesthesia, and the interscapular BAT depots were obtained. Total RNA was isolated using the TriPure isolation reagent (Roche Diagnostics, Mannheim, Germany) and contaminating genomic DNA was removed by the RQ1 RNase-free DNase (Promega Benelux, Leiden, Netherlands), according to the manufacturers' instructions. RNA quality was assessed by an Agilent 2100 Bioanalyzer (Agilent Technologies, Amstelveen, Netherlands). A TruSeq RNA Library Prep Kit v2 (Illumina, Eindhoven, Netherlands) was used for library preparation according to the manufacturer's instructions.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2021-01-16T04:08:47Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-01-22T14:28:20Z
attributes.tag: External Id
attributes.value: SAMEA6497486
attributes.tag: INSDC center name
attributes.value: Dept. of Internal Medicine Erasmus MC
attributes.tag: INSDC first public
attributes.value: 2021-01-16T04:08:47Z
attributes.tag: INSDC last update
attributes.value: 2020-01-22T14:28:20Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8717:Sample 2
attributes.tag: age
attributes.value: 16
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: individual
attributes.value: M2
attributes.tag: organism part
attributes.value: brown adipose tissue
attributes.tag: sample name
attributes.value: E-MTAB-8717:Sample 2
attributes.tag: sampling site
attributes.value: interscapular fat pad
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: C57BL/6J