organism: Homo sapiens
description: Protocols: We collected inflamed colonic and ileal biopsies, as well as blood (of which PBMCs were isolated and sorted through FACS) prior to the initiation of anti-TNF and Vedolizumab therapy. All recruited Crohn's disease patients had endoscopy-proven active disease (SES-CD > 7, and > 4 in patients with pure ileal involvement); all included UC patients had a Mayo endoscopic subscore 2-3. Total RNA from lysed inflamed biopsies and lysed sorted CD4+ and CD14+ cells was extracted using the AllPrep DNA/RNA Mini kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions. The integrity and quantity of all RNA was assessed with a 2100 Bioanalyzer (Agilent, Waldbronn, Germany) and a Nanodrop ND-1000 spectrophotometer (Thermo Scientific, Waltham, Massachusetts, USA). Library preparation was performed using the TruSeq Stranded mRNA protocol (Illumina, San Diego, USA) according to the manufacturer's instructions.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-01-03T15:59:20Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-04-26T19:15:22Z
attributes.tag: External Id
attributes.value: SAMEA5581214
attributes.tag: INSDC center name
attributes.value: Department of Gastroenterology, University Hospitals Leuven, KU Leuven, Leuven, Belgium Department of Chronic Diseases, Metabolism and Ageing, KU Leuven, Leuven, Belgium
attributes.tag: INSDC first public
attributes.value: 2020-01-03T15:59:20Z
attributes.tag: INSDC last update
attributes.value: 2019-04-26T19:15:22Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7914:GC049969
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: clinical information
attributes.value: endoscopic remission
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: Crohn's disease
attributes.tag: individual
attributes.value: Patient 9
attributes.tag: organism part
attributes.value: ileum
attributes.tag: sample name
attributes.value: E-MTAB-7914:GC049969
attributes.tag: scientific_name
attributes.value: Homo sapiens