organism: Mus musculus
description: Protocols: 1mg/kg for 7 consecutive days Total RNA was extracted from FACS-isolated GFP+ adipocytes or preadipocytes using the RNeasy Micro Kit (Qiagen). RNA quality (RIN: RNA Integrity Number) was determined by Bioanalyzer (Agilent Technologies) and samples with RIN of >7.0 were subjected to RNA-seq. Libraries were constructed from minimum of 22 ng of total RNA as previously described (Nature 504 (7478), 163-167)
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2016-08-25T17:01:16Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-09T09:21:25Z
attributes.tag: External Id
attributes.value: SAMEA3627886
attributes.tag: INSDC center name
attributes.value: Diabetes Center, University of California, San Francisco (UCSF), San Francisco, California, USA.
attributes.tag: INSDC first public
attributes.value: 2016-08-25T17:01:16Z
attributes.tag: INSDC last update
attributes.value: 2018-03-09T09:21:25Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-3978:Day5-C
attributes.tag: age
attributes.value: 8-12 weeks
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: adipocyte
attributes.tag: common name
attributes.value: house mouse
attributes.tag: compound
attributes.value: CL316,243
attributes.tag: dose
attributes.value: 1mg/kg for 7 consecutive days
attributes.tag: genotype
attributes.value: Ucp1Cre/+;Rosa26-GFP reporter
attributes.tag: organism part
attributes.value: inguinal white adipose tissue
attributes.tag: sample name
attributes.value: E-MTAB-3978:Day5-C
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: time
attributes.value: 5