organism: Gallus gallus
description: Protocols: Workspace and dissection tools were thoroughly cleaned using RNaseZap (Ambion), then rinsed in diethyl pyrocarbonate (depc, SIGMA) treated water. Embryos at the desired stage were dissected into fresh depc treated 1xPBS (Phosphate buffered saline). Posterior forewing/foreleg sections were cut out from the embryo using micro-dissection scissors and placed in RNAlater and stored at -80°C (Ambion). Posterior forelimb sections were collected from embryonic chickens at embryonic day 10.5 E10.5 (If cyclopmaine treated, this was carried out at Hamburger Hamilton Stage 19, E4.5). Samples were collected in triplicate (with 10 posterior limb sections pooled per replicate). Pb sample tissue was collected from Pekin bantam feathered leg chickens. All other samples are of a wild-type background (Bovans Brown chicken eggs). Addition of 5ul cyclopamine at Hamburger Hamilton stage HH19. Samples were homogenised in 1ml of Trizol. An equal volume of 100% ethanol was added and samples were purified using Zymo Research Direct-zol RNA Miniprep Kit. TruSeq Stranded Total RNA and Ribo-Zero. Enzymatic fragmentation by Illumina Elute, Fragment, Prime mix
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-12-04T04:02:31Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-12-14T16:26:26Z
attributes.tag: External Id
attributes.value: SAMEA5176913
attributes.tag: INSDC center name
attributes.value: UNIVERSITY OF SHEFFIELD
attributes.tag: INSDC first public
attributes.value: 2019-12-04T04:02:31Z
attributes.tag: INSDC last update
attributes.value: 2018-12-14T16:26:26Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7520:pb2
attributes.tag: breed
attributes.value: Pekin Bantam
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: mesenchyme and epithelium
attributes.tag: common name
attributes.value: chicken
attributes.tag: developmental stage
attributes.value: embryonic day 10.5
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: posterior foreleg
attributes.tag: sample name
attributes.value: E-MTAB-7520:pb2
attributes.tag: scientific_name
attributes.value: Gallus gallus