organism: Homo sapiens
description: Protocols: Peripheral (isolated from peripheral blood) and tumor (melanoma) associated B cells (PBMCB and TAB, respectively) were EBV-immortalized (pre-immortalization = NATIVE) and either exposed to melanoma conditioned medium (MCM) or control medium (MOCK). TRI Reagent, Sigma, Cat. Nr. T9424 RNA-seq libraries were prepared with the TruSeq Stranded mRNA LT sample preparation kit (Illumina) using both, Sciclone and Zephyr liquid handling robotics (PerkinElmer). Library concentrations were quantified with the Qubit Fluorometric Quantitation system (Life Technologies) and the size distribution was assessed using the Experion Automated Electrophoresis System (Bio-Rad).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-08-14T17:04:07Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-06-12T17:03:49Z
attributes.tag: External Id
attributes.value: SAMEA5713984
attributes.tag: INSDC center name
attributes.value: EMBL-European Bioinformatics Institute
attributes.tag: INSDC first public
attributes.value: 2019-08-14T17:04:07Z
attributes.tag: INSDC last update
attributes.value: 2019-06-12T17:03:49Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-74721560354963:P2_PBMC_B_MCM_S51254
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: EBV-transformed B-cell
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: not available
attributes.tag: disease
attributes.value: metastatic melanoma
attributes.tag: growth condition
attributes.value: melanoma conditioned medium
attributes.tag: individual
attributes.value: P4
attributes.tag: medium donor
attributes.value: P4
attributes.tag: organism part
attributes.value: blood
attributes.tag: sample name
attributes.value: E-MTAB-74721560354963:P2_PBMC_B_MCM_S51254
attributes.tag: sampling site
attributes.value: normal tissue
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: not available