organism: Homo sapiens
description: Protocols: Lymphoblast cell lines were obtained for seven primate species. All cell lines were transformed by the Epstein Barr virus except for the macaque cell lines that were transformed by a herpesvirus. Cells were grown in suspension at a confluency of 200 000-1 x106 cells/ml in RPMI1640 media supplemented with 10% Fetal Bovine Serum and 2 mM L-glutamine. C57BL/6J mice were housed in the Biological Resources Unit under UK Home Office licensing. Tissue was obtained from at least two independent males and cross-linked as described in (Schmidt, Methods,2009). Male and female human tissue samples were obtained from biopsied tissue collected at Addenbrooke's Hospital, Cambridge, and were provided by the Biobank under human tissue license 08/H0308/117. Liver tissue was also obtained from the Liver Tissue Distribution Program (NIDDK contract number N01-DK-9-2310) at the University of Pittsburgh. 1x10^8 cells were cross-linked with 1% formaldehyde as previously described (Schmidt, Methods,2009). ChIP-seq assays were performed as previously described (Schmidt, 2009). Protein-bound DNA was immunoprecipitated with antibodies against CTCF (Millipore, 07-729), or YY1 (Santa Cruz, sc-281).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2013-04-22T17:03:04Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T16:17:58Z
attributes.tag: External Id
attributes.value: SAMEA1877853
attributes.tag: INSDC center name
attributes.value: EFPL
attributes.tag: INSDC first public
attributes.value: 2013-04-22T17:03:04Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T16:17:58Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-1511:Human liver 1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: human
attributes.tag: organism part
attributes.value: liver
attributes.tag: sample name
attributes.value: E-MTAB-1511:Human liver 1
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: strain
attributes.value: HH1320