organism: Homo sapiens
description: Protocols: PBMCs were isolated from three individuals. Cells from these biological replicates were exposed to infected RBCs, uninfected RBCs, or RPMI prior to stimulation with PAM3CSK4 (10g/mL) for 0, 4, and 12 hours. Total RNA was extracted using the Aurum Total RNA Mini Kit (BioRad). For each sample, 0.5 g of total RNA was used to prepare RNA-seq libraries using the TruSeq Stranded Total RNA Library Prep Human/Mouse/Rat kit and the TruSeq RNA single indexes Set A (Illumina) according to the manufacturer's instructions.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-05-15T04:06:09Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-05-13T21:29:32Z
attributes.tag: External Id
attributes.value: SAMEA6831294
attributes.tag: INSDC center name
attributes.value: Department of Medicine University of Massachusetts Medical School
attributes.tag: INSDC first public
attributes.value: 2020-05-15T04:06:09Z
attributes.tag: INSDC last update
attributes.value: 2020-05-13T21:29:32Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-9066:Sample 8
attributes.tag: age
attributes.value: 27
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: peripheral blood mononuclear cell
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: normal
attributes.tag: growth condition
attributes.value: erythrocytes infected with P. falciparum 3D7
attributes.tag: individual
attributes.value: 1
attributes.tag: organism part
attributes.value: blood
attributes.tag: replicate
attributes.value: 1
attributes.tag: sample name
attributes.value: E-MTAB-9066:Sample 8
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: female
attributes.tag: stimulus
attributes.value: PAM3/CSK4; 10 microgram per milliliter