organism: Mus musculus
description: Protocols: Gli1cre/Tom mice were treated with 2 x 3mg tamoxifen i.p. at 7 weeks of age. One week later, full epidermal cells were isolated as described previously (Jaks et al., Nat Genetics. 2008). In brief, dorsal skin was harvested, dermal and adipose tissue was removed, the tissue was cut into strips and subsequently digested in trypsin. Epidermal cells were scraped off the dermis and a single-cell suspension was obtained by magnetic stirring. The cells were stained with Sca1 and Cd34 antibodies and life-sorted into a fraction of traced cells from the interfollicular epidermis (Gli1/Tomato+ / Sca1+) and a fraction of cells from the hair follicle (Gli1/Tomato+ / Sca1-) using a FACSAria system. Single-cells from both the hair follicle and interfollicular epidermis fractions were captured separately on Fluidigm C1 Autoprep microfluidic systems. Cell lysis and cDNA synthesis were subsequently performed using a custom protocol (STRT-C1) as described previously (Islam et al., Nat Methods. 2014). Library preparation from single-cell cDNA was performed using a tagmentation-based approach as described in detail in Islam et al., 2014.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-01-03T15:59:16Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-12-19T10:16:42Z
attributes.tag: External Id
attributes.value: SAMEA5190082
attributes.tag: INSDC center name
attributes.value: Center for Innovative Medicine & Department for Biosciences and Nutrition, Karolinska Institutet
attributes.tag: INSDC first public
attributes.value: 2020-01-03T15:59:16Z
attributes.tag: INSDC last update
attributes.value: 2018-12-19T10:16:42Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6031:1772099239-H01
attributes.tag: age
attributes.value: 8
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: keratinocyte
attributes.tag: common name
attributes.value: house mouse
attributes.tag: genotype
attributes.value: Gli1cre(het)-Tomato(het)
attributes.tag: inferred cell type
attributes.value: removed
attributes.tag: organism part
attributes.value: epidermis
attributes.tag: phenotype
attributes.value: Gli1-Tomato+/Sca1+
attributes.tag: sample name
attributes.value: E-MTAB-6031:1772099239-H01
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: C57BL/6