organism: Arabidopsis thaliana
attributes.tag: comment
attributes.value: Wild-type, pin6-2 (SALK_046393), and myb7-2 (SALK_030969) A. thaliana plants were grown on soil in individual pots under a 16h day (150 microE m-2 s-1 photosynthetically active radiation)/8h night cycle for 30-35 days as described previously (Kram et al. 2009). RNA was isolated from pooled lateral nectaries of Stage 14-15 flowers as previously described (~300 pooled nectaries per sample; Kram et al. 2009). The pin6-2 and myb57-2 mutants partially phenocopy each other in producing less nectar and often missing one or both short stamen.
attributes.tag: tissue
attributes.value: lateral nectaries
attributes.tag: dev-stage
attributes.value: 14-15 flowers
attributes.tag: substrate
attributes.value: peat-based growth medium with vermiculite and perlite (Pro-Mix BX)
attributes.tag: temperature
attributes.value: 23C
attributes.tag: age
attributes.value: 30-35 days
attributes.tag: light_intensity
attributes.value: 150 microE m-2 s-1 photosynthetically active radiation
attributes.tag: genotypes
attributes.value: Columbia-0 pin6-2
attributes.tag: ecotype
attributes.value: Col-0