organism: Mus musculus
description: Protocols: Approximately 10,000-90,000 Lgr5-GFPHi ISCs or Lgr5-GFPLow progenitors from 3 pairs of WT or Lkb1Lgr5-KO mice 5 days after tamoxifen injection were isolated by flow cytometry. Tamoxifen induction on Lgr5GFP-Cre/+;Lkb1+/+;R26Td/+ mouse leads to the activation of TdTomato lineage tracing while Lkb1 remains wildtype (Lkb1-WT) in intestinal Lgr5+ stem cells and their progeny in comparison to tamoxifen induction on Lgr5GFP-Cre/+;Lkb1fl/fl;R26Td/+ mouse leads to the activation of TdTomato lineage tracing and inactivation of Lkb1 (Lkb1-KO) in intestinal Lgr5+ stem cells and their progeny. Total RNA was extracted using TRI Reagent (MRC, TR118) Ovation Universal RNA-Seq System kit was used for lllumina library preparations (NuGEN Technologies., CA, USA). Purified total RNAs were used for ribosomal removal as outlined in the kit manual. Libraries were purified with AMPure XP beads (Beckman Coulter Inc., MA, USA).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-12-30T04:03:40Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-13T14:19:00Z
attributes.tag: External Id
attributes.value: SAMEA104692582
attributes.tag: INSDC center name
attributes.value: UoH
attributes.tag: INSDC first public
attributes.value: 2019-12-30T04:03:40Z
attributes.tag: INSDC last update
attributes.value: 2018-03-13T14:19:00Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6501:Sample 6
attributes.tag: age
attributes.value: 3
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: intestinal stem cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: Lgr5GFP-Cre/+;Lkb1+/+;R26Td/+
attributes.tag: individual
attributes.value: low_2138_wt
attributes.tag: organism part
attributes.value: small intestine
attributes.tag: phenotype
attributes.value: Lkb1 wildtype; Lgr5-GFP low
attributes.tag: sample name
attributes.value: E-MTAB-6501:Sample 6
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: male
attributes.tag: stimulus
attributes.value: tamoxifen; i.p 0.15 milligram per gram body weight
attributes.tag: strain
attributes.value: C57BL/6 x CD1