organism: Saccharomyces cerevisiae
description: Protocols: S. cerevisiae cells were grown according to standard procedures at 25 °C in synthetic complete medium with bases and amino acids omitted as necessary for selection and with glucose (2%) as a carbon source. The pCM185 vector (CEN, TRP1) from Euroscarf was used as backbone to create the Doxycycline-regulated (Tet off) Rho expression construct (pCM185-Rho-NLS) (Mosrin-Huaman et al., 2009). Strains transformed by Rho expression vector were grown under repressing conditions (5 µg/mL of Doxycycline) at 25°C in YNB medium supplemented with SC-TRP (Synthetic Complete Supplement Mixture from MP Biomedicals) and glucose as carbon source (final concentration 2%). To allow the expression of Rho, the yeast cells were grown the same medium but without Doxycycline. Growth was performed during 16 hours incubation at 25°C. Total RNA extraction was performed using the acidic hot phenol/chloroform method Libraries preparations were made by I2BC high-throughput sequencing platform (Gif sur Yvette) using Illumina ScriptSeq protocol after RiboZero treatment.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-12-04T04:02:31Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-12-18T14:33:59Z
attributes.tag: External Id
attributes.value: SAMEA5187604
attributes.tag: INSDC center name
attributes.value: CNRS
attributes.tag: INSDC first public
attributes.value: 2019-12-04T04:02:31Z
attributes.tag: INSDC last update
attributes.value: 2018-12-18T14:33:59Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7538:WT_NI_1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: baker's yeast
attributes.tag: genotype
attributes.value: pCM185-Rho-NLS
attributes.tag: growth condition
attributes.value: YNB medium supplemented with SC-TRP and 2% glucose
attributes.tag: phenotype
attributes.value: wild type phenotype
attributes.tag: sample name
attributes.value: E-MTAB-7538:WT_NI_1
attributes.tag: scientific_name
attributes.value: Saccharomyces cerevisiae
attributes.tag: stimulus
attributes.value: -doxycycline
attributes.tag: strain
attributes.value: BMA41