organism: freshwater metagenome
description: Strand-specific freshwater metatranscriptome RE-16aug2020-ssRNA Sampling was performed on the 16th of August 2020 at 9:00 in Rimov reservoir 0.5m, Czech Republic, (48°50'54.4N 14°29'16.7E) using a hand-held vertical Friedinger (2 L) sampler. A total of 20 L of water were collected from a depth of 0.5m and immediately transported to the laboratory. Sample was subjected to rRNA depletion and used for stranded library preparation. Strand-specificity was achieved by incorporation of dUTPs instead of dTTPs in the second-strand cDNA followed by digestion of dUTPs by uracil-DNA glycosylase to prevent PCR amplification of this strand. Paired-end (PE 150-bp) sequencing was carried out using a Novaseq 6000 platform.
attributes.tag: ENA first public
attributes.value: 2021-01-14
attributes.tag: ENA last update
attributes.value: 2020-11-25
attributes.tag: External Id
attributes.value: SAMEA7625098
attributes.tag: INSDC center alias
attributes.value: BIOLOGY CENTRE ASCR, V.V.I., INSTITUTE OF HYDROBIO
attributes.tag: INSDC center name
attributes.value: BIOLOGY CENTRE ASCR, V.V.I., INSTITUTE OF HYDROBIO
attributes.tag: INSDC first public
attributes.value: 2021-01-14T17:19:15Z
attributes.tag: INSDC last update
attributes.value: 2020-11-25T15:13:56Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: RE-16aug2020-ssRNA
attributes.tag: sample name
attributes.value: RE-16aug2020-ssRNA