organism: Mus musculus
description: Protocols: Ventricles were freshly dissected in cold PBS and snap frozen on liquid nitrogen. Heart tissue was homogenized in TRIZOL (Ambion, 15596026) using a Tissue Lyser LT (Qiagen). RNA was purified using the columns from RNeasy Mini Kit (Qiagen, 74104) according to the manufacturer instructions. Sequencing libraries were prepared using TrueSeq Stranded RNA LT Kit Ribo-Zero Gold (Illumina, 15032619).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-01-03T15:59:17Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-02-14T16:57:15Z
attributes.tag: External Id
attributes.value: SAMEA5339791
attributes.tag: INSDC center name
attributes.value: Max Planck Institute for Molecular Biomedicine Regulatory Genomics Laboratory
attributes.tag: INSDC first public
attributes.value: 2020-01-03T15:59:17Z
attributes.tag: INSDC last update
attributes.value: 2019-02-14T16:57:15Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7686:Control_3
attributes.tag: age
attributes.value: 12 weeks
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: Ephb4 Flox/Flox; +/+
attributes.tag: individual
attributes.value: C_3
attributes.tag: organism part
attributes.value: heart ventricles
attributes.tag: sample name
attributes.value: E-MTAB-7686:Control_3
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: Bl6 and mixed background