organism: Candida parapsilosis
description: Protocols: The strain CLIB214 (CBS604) The CLIB214 (CBS604) cells were pre-cultivated in an SD medium (0.67% [wt/vol] yeast nitrogen base w/o amino acids (Difco), 2% [wt/vol] glucose) for 24 h at 28 °C, then washed in water, inoculated (OD600 ~ 0.2-0.3) into an SMix medium (0.67% [wt/vol] yeast nitrogen base w/o amino acids (Difco), 3.3 mM 3-hydroxybenzoate, 3.3 mM 4-hydroxybenzoate and 3.3 mM hydroquinone) for additional 24 h at 28 °C. The pre-culture was inoculated (OD600 ~ 0.3) into synthetic media (0.67% [wt/vol] yeast nitrogen base w/o amino acids (Difco)) containing 2% [wt/vol] galactose (SGal) or 10 mM hydroxyaromatic compound (i.e. 3-hydroxybenzoate (S3OH), 4-hydroxybenzoate (S4OH), 3,4-dihydroxybenzoate (S34diOH) or hydroquinone (SHyd)) as a sole carbon source and cultivated at 28 °C till OD600 ~ 1. Total RNA was extracted using the hot acid phenol protocol essentially as described in Collart and Oliviero (2001) and purified using an Uneasy mini kit (Qiagen); (RIN 7.4-9.3). Reference: Collart, M.A. and Oliviero, S. (2001) Preparation of yeast RNA. Curr. Protoc. Mol. Biol. Chapter 13: Unit 13.12. The sequencing library was prepared using a TruSeq Stranded mRNA LT Sample Prep Kit (2x 151).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2021-01-15T04:09:30Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-08-17T13:04:37Z
attributes.tag: External Id
attributes.value: SAMEA7192482
attributes.tag: INSDC center name
attributes.value: Department of Computer Science Faculty of Mathematics, Physics and Informatics Comenius University in Bratislava, Slovakia
attributes.tag: INSDC first public
attributes.value: 2021-01-15T04:09:30Z
attributes.tag: INSDC last update
attributes.value: 2020-08-17T13:04:37Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-9443:S4OH_2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: growth condition
attributes.value: S4OH
attributes.tag: organism part
attributes.value: whole organism
attributes.tag: sample name
attributes.value: E-MTAB-9443:S4OH_2
attributes.tag: scientific_name
attributes.value: Candida parapsilosis
attributes.tag: strain
attributes.value: CLIB 214 (CBS 604)