organism: Homo sapiens
description: Protocols: Fresh biopsies from both inflamed and non-inflamed segments were obtained during endoscopy from 8 patients with active UC (endoscopic Mayo subscore of ≥2) and an accessible border of inflammation. Crypts were isolated and cultured as organoids for 4 weeks with weekly mechanical splitting. RNA was extracted from snapfrozen biopsies biopsies, isolated crypts and 1 and 4 week old organoids. Total RNA was extracted using the RNeasy Mini Kit following the manufacturer instructions (Qiagen, Hilden, Germany). RNA was quantified by Nanodrop and the integrity was evaluated using Agilent Bio-analyzer 2100. The library construction was performed by the Lexogen QuantSeq 3' mRNA-Seq Library Kit FWD (Lexogen, Vienna, Austria)
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-01-03T15:59:19Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-04-15T11:20:34Z
attributes.tag: External Id
attributes.value: SAMEA5567293
attributes.tag: INSDC center name
attributes.value: Department of Chronic Diseases, Metabolism and Ageing (CHROMETA), Translational Research Center for Gastrointestinal Disorders (TARGID), KU Leuven, Leuven, Belgium
attributes.tag: INSDC first public
attributes.value: 2020-01-03T15:59:19Z
attributes.tag: INSDC last update
attributes.value: 2019-04-15T11:20:34Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7860:GC057652
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: ulcerative colitis
attributes.tag: individual
attributes.value: Patient 5
attributes.tag: organism part
attributes.value: colon
attributes.tag: sample name
attributes.value: E-MTAB-7860:GC057652
attributes.tag: sampling site
attributes.value: inflammation
attributes.tag: scientific_name
attributes.value: Homo sapiens