organism: Nematostella vectensis
attributes.tag: source_name
attributes.value: gastrulae (24h)
attributes.tag: development stage
attributes.value: gastrulae (24h)
attributes.tag: chip antibody
attributes.value: Nematostella p300/CBP
attributes.tag: chip antibody source
attributes.value: We used a 146 aa peptide of the C-terminus of Nematostella p300 protein, including a N-terminal 10x His tag for immunization of two rabbits each. The p300 peptide sequence was HPNSQAQQPPGQHYQAKQMQPRLMSPQAPPPMSPQQGGLPSQSPRPVMSPQPHANTLSPRQQTMSPAHPQQSLTSPRPAQSPHQSHMDPSNLGDKPNLFTQPLSARMTLTPLAAPSESDMSLGQEDSETVPPLTPQDQLTKFVENL. P300 antisera and purified p300 antibodies were generated by Primm (www.primmbiotech.com).
attributes.tag: fragmentation
attributes.value: sonication
attributes.tag: library method
attributes.value: ChIP-Seq
attributes.tag: read length
attributes.value: 50