organism: Homo sapiens
description: Protocols: Cell culture dishes were harvested. Monocytes obtained from 3 individual donors were cultured for 6 days in RPMI 5% FCS 1% sodium pyruvate, differentiating them into monocyte-derived-macrophages. Treated for 3h with solvent (LSM) 50 µM (Arachidonic Acid Biomol Cay90010-100), 20 ng/ml IFNb (Interferon beta1a, human recombinant (rHuIFN-beta1a-CHO)Biomol Art# 86421.20) or both. NucleoSpin RNA Kit (Macherey-Nagel, 740955.250); DNA-free™ DNA Removal Kit (ThermoFisher/Ambion, AM1906); according to manufacturer's protocol Libraries were created using the Illumina Truseq mRNA kit v2 used according to the manufacturer's instructions.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-12-10T04:04:51Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-02-28T13:45:46Z
attributes.tag: External Id
attributes.value: SAMEA6595729
attributes.tag: INSDC center name
attributes.value: Philipps University Marburg
attributes.tag: INSDC first public
attributes.value: 2020-12-10T04:04:51Z
attributes.tag: INSDC last update
attributes.value: 2020-02-28T13:45:46Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8833:ST042
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: monocyte derived macrophage
attributes.tag: cell type
attributes.value: monocyte
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: blood
attributes.tag: sample name
attributes.value: E-MTAB-8833:ST042
attributes.tag: scientific_name
attributes.value: Homo sapiens