organism: Mus musculus
description: Protocols: The preparation of the material was as described in Kutejova et al., 2008. Second branchial arches were dissected (the whole tissue), crosslinked and frozen in order to pool enough for ChIP. Kutejova E, Engist B, Self M, Oliver G, Kirilenko P, Bobola N. Six2 functions redundantly immediately downstream of Hoxa2. Development. 2008. 135(8):1463-70. PMID: 18321982. Diapure kit (Diagenode) ChIP-seq was performed as described in Donaldson et al., 2012.  'Rep1' used 20ug of a anti-Hoxa2 rabbit polyclonal antibody was generated by Biogenes (Berlin, Germany).  'Rep2' used 20ug of previously generated Ab detailed in Kutejova et al., 2009. 60 arches were used for 'rep1' and 'rep2'. Library preparation was performed using the Microplex Diagenode kit (Diagenode). Donaldson I, Amin S, Hensman J, Kutejova E, Rattray M, Lawrence N, Hayes A, Ward C and Bobola N. Genome-wide occupancy links Hoxa2 to Wnt-beta-catenin signaling in mouse embryonic development. Nucleic Acids Res. 2012. 40(9):3990-4001 Kutejova et al, 2009 DOI:10.1242/dev.017624).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-01-06T17:04:11Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-03-12T13:46:55Z
attributes.tag: External Id
attributes.value: SAMEA5418139
attributes.tag: INSDC center name
attributes.value: UNIVERSITY OF MANCHESTER
attributes.tag: INSDC first public
attributes.value: 2020-01-06T17:04:11Z
attributes.tag: INSDC last update
attributes.value: 2019-03-12T13:46:55Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7766:Hoxa2 Input BA2 rep2
attributes.tag: age
attributes.value: 11.5
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: embryo stage
attributes.tag: genotype
attributes.value: WT
attributes.tag: individual
attributes.value: pooled
attributes.tag: organism part
attributes.value: second branchial arch
attributes.tag: sample name
attributes.value: E-MTAB-7766:Hoxa2 Input BA2 rep2
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: mixed
attributes.tag: strain
attributes.value: CD1