organism: Homo sapiens
description: Protocols: Nasal brushing samples from inferior turbinates of healthy and nasal polyps of AERD patients of cohort 1 were collected under anterior rhinoscopy using the Rhino-Pro Curette (Arlington Scientific, Springville, UT, USA) as described previously (Dhariwal J. et al, American Journal of Respiratory and Critical Care Medicine, 2017; Proud D. et al, The Journal of Immunology, 2004). Nasal brushing samples were directly transferred into RNAprotect (Qiagen, Hilden, Germany) and stored at -80 °C until RNA isolation. Total RNA was extracted according to the manufacturer's instructions (Quick-RNA MicroPrep Kit, Zymo Research, Irvine, CA, USA). RNA integrity number (RIN) was determined with the Agilent 2100 BioAnalyzer (RNA 6000 Nano Kit, Agilent). For library preparation, 1 μg of RNA was depleted for cytoplasmatic rRNAs , fragmented, and reverse transcribed with the Elute, Prime, Fragment Mix . A-tailing, adaptor ligation, and library enrichment were performed as described in the High Throughput protocol of the TruSeq RNA Sample Prep Guide (Illumina). RNA libraries were assessed for quality and quantity with the Agilent 2100 BioAnalyzer and the Quant-iT PicoGreen dsDNA Assay Kit (Life Technologies).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-01-03T15:59:20Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-05-14T15:31:55Z
attributes.tag: External Id
attributes.value: SAMEA5607360
attributes.tag: INSDC center name
attributes.value: Center of Allergy & Environment (ZAUM) Technische Universitat and Helmholtz Zentrum Munchen
attributes.tag: INSDC first public
attributes.value: 2020-01-03T15:59:20Z
attributes.tag: INSDC last update
attributes.value: 2019-05-14T15:31:55Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7962:MUC7201_S24
attributes.tag: age
attributes.value: 53
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: aspirin-exacerbated respiratory disease
attributes.tag: individual
attributes.value: 75
attributes.tag: organism part
attributes.value: nasal cavity
attributes.tag: sample name
attributes.value: E-MTAB-7962:MUC7201_S24
attributes.tag: sampling site
attributes.value: nasal cavity polyp
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: female