organism: Mus musculus
description: TNG_MKOS MEFs are overexpressed with ESRP2 by retroviral transductions.
attributes.tag: strain
attributes.value: 129_TNG MKOS
attributes.tag: dev_stage
attributes.value: Embryonic
attributes.tag: sex
attributes.value: not applicable
attributes.tag: tissue
attributes.value: Embryonic Fibroblasts
attributes.tag: cell_type
attributes.value: induced pluripotent stem cells
attributes.tag: growth_protocol
attributes.value: 3 to 15% of TNG_MKOS MEFs together with 97 to 85% WT MEFs were transduced with retroviral particles expressing transgenes for 4 hrs and grown in GMEM+1000 units of LIF, 1ug/ml Doxycycline, 10 ug/ml Vitamin C, 10% FBS, 1X non-essential medium, 1X pen/strep, 0.1 mM 2mercaptoethanol, 2mM L- glutamine. mOrange positive cells for the first five days and Nanog-GFP positive for day 15 were collected by FACS for sample preparation.
attributes.tag: sample_type
attributes.value: SRA
attributes.tag: treatment
attributes.value: Around 350,000 Nanog-GFP cells were sorted by flow cytometry for preparing mRNA-seq samples as mentioned in Mittal et al., Nat Commun 2017; 8: 457.
attributes.tag: BioSampleModel
attributes.value: Model organism or animal