organism: Arabidopsis thaliana
description: Protocols: Roots/root parts were cut off and directly frozen in liquid nitrogen. Arabidopsis thaliana plants were grown from seeds on ½ MS medium in squared Petri dishes, which were placed vertically in a growth chamber. Plants were kept under long-day conditions (16 h light, 120-130 µE/ 8 h dark) at 24 °C during light and 22 °C during darkness. Humidity was kept between 60-80%. L. bicolor was grown from culture on cellophane and P20 medium for 14 days at 22 °C in darkness. Per bi-compartmented squared Petri dish, 15 4-days-old A. thaliana seedlings were co-incubated with 4 14-days-old L. bicolor plugs. Control seedlings were grown with cellophane and P20. Arabidopsis RNA was extracted using RNAeasy extraction kit (Qiagen) according to the manufacturer's protocol. Poly-A RNA was enriched using the NEBNext Poly (A) mRNA Magnetic Isolation Module (New England Biolabs (NEB), Ipswich, Massachusetts, USA). Sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB) according to manufacturer's instructions.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-12-10T04:04:48Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-01-16T16:13:08Z
attributes.tag: External Id
attributes.value: SAMEA6484057
attributes.tag: INSDC center name
attributes.value: Georg-August-Universitaet Buesgen-Institut Department of Forest Botany and Tree Physiology
attributes.tag: INSDC first public
attributes.value: 2020-12-10T04:04:48Z
attributes.tag: INSDC last update
attributes.value: 2020-01-16T16:13:08Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8682:Sample 5
attributes.tag: age
attributes.value: 6
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: thale cress
attributes.tag: developmental stage
attributes.value: plant structure development stage
attributes.tag: ecotype
attributes.value: Col-0
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: growth condition
attributes.value: long day length regimen
attributes.tag: organism part
attributes.value: root
attributes.tag: sample name
attributes.value: E-MTAB-8682:Sample 5
attributes.tag: scientific_name
attributes.value: Arabidopsis thaliana