organism: Homo sapiens
description: Protocols: Colon cancer patient-derived xenograft (PDX) models models were processed to single cells and sorted by FACS (BD FACS Aria II) for ALDH activity (Aldefluor assay) and DAPI. ALDH Negative and ALDH Positive cells from each PDX model were collected. ALDH Negative and ALDH Positive cells from each PDX model were collected and lysed in RLT buffer and processed for RNA using the RNeasy Mini Plus RNA extraction kit (Qiagen). Samples were processed using Illumina's TrueSeq RNA protocol
attributes.tag: ENA first public
attributes.value: 2021-01-11
attributes.tag: ENA last update
attributes.value: 2020-12-11
attributes.tag: External Id
attributes.value: SAMEA7705421
attributes.tag: INSDC center alias
attributes.value: Charite - Universitatsmedizin Berlin
attributes.tag: INSDC center name
attributes.value: Charite - Universitatsmedizin Berlin
attributes.tag: INSDC first public
attributes.value: 2021-01-11T04:04:54Z
attributes.tag: INSDC last update
attributes.value: 2020-12-11T13:59:59Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8927:77_302-CB-M-MF_XENO_ALDH-POS
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: colorectal cancer cell
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: colon carcinoma
attributes.tag: fraction
attributes.value: ALDH Positive
attributes.tag: growth condition
attributes.value: Xenograft in female nude-/- mice
attributes.tag: individual
attributes.value: Patient 302-CB-M-MF
attributes.tag: sample name
attributes.value: E-MTAB-8927:77_302-CB-M-MF_XENO_ALDH-POS