organism: Homo sapiens
description: Protocols: Clonal HAP1 KO clones were obtained from Horizon Discovery. Cells were grown in IMDM (GIBCO, #21980-032) supplemented with 10% FCS (GIBCO, #10270) and harvested at 70-90% confluency. Total RNA was isolated from cells using the Direct-zol 96 RNA kit (Zymo research) according to the manufacturer's protocol. In brief, 2-3 * 106 cells were lysed in 1 ml Trizol. Ethanol was added to the supernatant and RNA purified using the Zymo-spin plate. RNA integrity was checked on a Fragment Analyzer (Advanced analytical) and prepared for sequencing using the NEBNext Ultra RNA Library Preparation Kit for Illumina (NEB) according to the manufacturer's instructions. DNA was amplified by 13-14 PCR cycles and 10-11 samples were pooled before sequencin
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-07-14T04:03:13Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-05-31T11:22:23Z
attributes.tag: External Id
attributes.value: SAMEA5671911
attributes.tag: INSDC center name
attributes.value: EMBL
attributes.tag: INSDC first public
attributes.value: 2019-07-14T04:03:13Z
attributes.tag: INSDC last update
attributes.value: 2019-05-31T11:22:23Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7061_2:Sample 45
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: HAP-1
attributes.tag: cell type
attributes.value: fibroblast
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: chronic myelogenous leukemia
attributes.tag: genetic modification
attributes.value: 104bp insertion in exon 5
attributes.tag: genotype
attributes.value: MTF2 CRISPR/Cas9 mediated frameshift mutation
attributes.tag: organism part
attributes.value: bone marrow
attributes.tag: phenotype
attributes.value: MTF2 homozygous knockout
attributes.tag: sample name
attributes.value: E-MTAB-7061_2:Sample 45
attributes.tag: scientific_name
attributes.value: Homo sapiens