organism: fish metagenome
description: Larval sablefish microbiome. 12-15 fish collected from rearing tanks Following surface sterilization, whole fish were homoginized and DNA was extracted using the Qiagen DNeasy PowerSoil kit and used for PCR amplification of 16S rRNA gene (V3-4) with primers 341F/806R. PCR products were pair-end sequenced (2 x 300 bp) on an Illumina MiSeq.
attributes.tag: host
attributes.value: sablefish, Anoplopoma fimbria
attributes.tag: isolation_source
attributes.value: Puget Sound, Washington, USA
attributes.tag: collection_date
attributes.value: 2019-06-03
attributes.tag: geo_loc_name
attributes.value: USA: NOAA research laboratory, Machester, Washington
attributes.tag: lat_lon
attributes.value: 47.571199 N 122.552665 W
attributes.tag: source_material_id
attributes.value: 6_3_19_Control_Tank3_Fish_16S_4h_16S
attributes.tag: BioSampleModel
attributes.value: Metagenome or environmental