organism: Mus musculus
description: Protocols: Cells were harvested by trypsinisation. Total RNA from cells were extracted using RNeasy Mini kit 74104 Qiagen, Manchester, UK), cDNA library preparation was carried out at Oxford Genomics Centre, The Wellcome Trust Centre for Human Genetics using PolyA+ RNA enrichment method for total RNA from cultured cells . mRNA fraction was selected from the total RNA before conversion to cDNA. Second strand cDNA synthesis incorporated dUTP. The cDNA was end-repaired, A-tailed and adapter-ligated. Prior to amplification, samples underwent uridine digestion. The prepared libraries were size selected, multiplexed and quality checked.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-11-30T11:02:52Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-06-04T14:00:18Z
attributes.tag: External Id
attributes.value: SAMEA4706952
attributes.tag: INSDC center name
attributes.value: The Institute of Cancer Research
attributes.tag: INSDC first public
attributes.value: 2018-11-30T11:02:52Z
attributes.tag: INSDC last update
attributes.value: 2018-06-04T14:00:18Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6846:Empc1 KO2 rep2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: embryonic cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: embryonic day 11.5
attributes.tag: genotype
attributes.value: Sox9 heterozygous knockout
attributes.tag: organism part
attributes.value: mammary bud
attributes.tag: sample name
attributes.value: E-MTAB-6846:Empc1 KO2 rep2
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: C57BL/6 x (CBA/Ca x C57BL/10) F6