organism: Homo sapiens
description: Protocols: Peripheral blood mononuclear cells were magnetically enriched for CD4+ T-cells with a kit (Miltenyi). T-cells were stained with antibodies to CD4, Il-7R, CD25, CXCR5, CCR6, CXCR3, CD161 and CCR5 and purified on a FACS Aria (BD). CD4+IL-7R+CD25-/lo helper T-cells were purified as CXCR5+CCR6+CXCR3-CD161-CCR5- TFH17-cells, CXCR5-CCR6+CXCR3-CD161+ or CCR5+Th17-cells or as CXCR5-CCR6+CXCR3-CD161-CCR5-CCR6SP T-cells RNA was isolated using mirVana Isolation Kit (ThermoFisher) Library construction was performed with the TruSeq RNA Sample Preparation Kit v2 (Illumina)
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-03-11T04:05:41Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-03-09T12:43:46Z
attributes.tag: External Id
attributes.value: SAMEA6615106
attributes.tag: INSDC center name
attributes.value: Istituto Nazionale Genetica Molecolare
attributes.tag: INSDC first public
attributes.value: 2020-03-11T04:05:41Z
attributes.tag: INSDC last update
attributes.value: 2020-03-09T12:43:46Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8862:SQ_2050
attributes.tag: age
attributes.value: not available
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: helper T cell
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: normal
attributes.tag: individual
attributes.value: 3
attributes.tag: organism part
attributes.value: blood
attributes.tag: phenotype
attributes.value: CXCR5-CCR6+CXCR3-CD161-
attributes.tag: sample name
attributes.value: E-MTAB-8862:SQ_2050
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: not available