organism: Mus musculus
description: Protocols: 2. We followed the standard procedures for cell collection. 50 million E14-TIR1 or SCC1-AID mESCs were mixed with 500K HEK293 cells before fixation. Cells were fixed for 10 minutes in 1% formaldehyde at room temperature. Formaldehyde was quenched by the addition of glycine to a final concentration of 125 µM. 1. Wild type E14 mES cells were grown on gelatin-coated plates in DMEM supplemented with 10% FBS, penicillin/streptomycin, 5 µM 2-Mercaptoethanol, 2 mM L-glutamine, non-essential amino-acids (ThermoFisher) and 10 ng/mL recombinant Leukaemia-Inhibitory Factor (LIF). 3. As described in King et al., 2017. 4. As described in King et al., 2017.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-01-03T15:59:19Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-03-26T15:36:24Z
attributes.tag: External Id
attributes.value: SAMEA5537176
attributes.tag: INSDC center name
attributes.value: Principal Investigator
attributes.tag: INSDC first public
attributes.value: 2020-01-03T15:59:19Z
attributes.tag: INSDC last update
attributes.value: 2019-03-26T15:36:24Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7817:Scc1-input-AUX-rep1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: ES-E14 cell
attributes.tag: cell type
attributes.value: mouse embryonic stem cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: date of experiment
attributes.value: 08.10.2018
attributes.tag: developmental stage
attributes.value: embryo
attributes.tag: genotype
attributes.value: E14_Scc1_AID
attributes.tag: sample name
attributes.value: E-MTAB-7817:Scc1-input-AUX-rep1
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: spike in cell line
attributes.value: HEK293 Homo sapiens cells
attributes.tag: strain
attributes.value: 129Ola
attributes.tag: treatment
attributes.value: Auxin treatment for 6 hours