organism: Homo sapiens
description: Protocols: We collected inflamed colonic and ileal biopsies, as well as blood (of which PBMCs were isolated and sorted through FACS) prior to the initiation of ustekinumab therapy. All recruited Crohn's disease patients had endoscopy-proven active disease (SES-CD > 7, and > 4 in patients with pure ileal involvement) and were ustekinumab naïve. Total RNA from inflamed biopsies and sorted CD4+ and CD14+ cells was extracted using the AllPrep DNA/RNA Mini kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions. The integrity and quantity of all RNA was assessed with a 2100 Bioanalyzer (Agilent, Waldbronn, Germany) and a Nanodrop ND-1000 spectrophotometer (Thermo Scientific, Waltham, Massachusetts, USA). Library preparation was performed using the TruSeq Stranded mRNA protocol (Illumina, San Diego, USA) according to the manufacturer's instructions.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-01-03T15:59:19Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-03-21T09:54:19Z
attributes.tag: External Id
attributes.value: SAMEA5523739
attributes.tag: INSDC center name
attributes.value: Dpt. Gastroenterology and Hepatology, University Hospitals Leuven, Leuven, BELGIUM Dpt. Chronic Diseases, Metabolism and Ageing, KU Leuven, Leuven, BELGIUM
attributes.tag: INSDC first public
attributes.value: 2020-01-03T15:59:19Z
attributes.tag: INSDC last update
attributes.value: 2019-03-21T09:54:19Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7799:GC061641
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: CD4
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: Crohn's disease
attributes.tag: individual
attributes.value: Patient_058
attributes.tag: organism part
attributes.value: blood
attributes.tag: response to treatment
attributes.value: Response
attributes.tag: sample name
attributes.value: E-MTAB-7799:GC061641
attributes.tag: scientific_name
attributes.value: Homo sapiens