organism: Zea mays subsp. parviglumis
attributes.tag: age
attributes.value: 6 weeks after planting
attributes.tag: dev_stage
attributes.value: vegetative phase
attributes.tag: cultivar
attributes.value: Ames 21812
attributes.tag: ecotype
attributes.value: originally collected from 2 km west of Teloloapan, Altitude 1860 m
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: geo_loc_name
attributes.value: USA:Aurora, SD
attributes.tag: organism
attributes.value: Zea mays ssp parviglumis
attributes.tag: sample_name
attributes.value: control
attributes.tag: sample_title
attributes.value: non-weedy control
attributes.tag: tissue
attributes.value: mature leaf tips
attributes.tag: biomaterial_provider
attributes.value: Sharon Clay
attributes.tag: collected_by
attributes.value: Stephanie Bruggeman
attributes.tag: collection_date
attributes.value: 2014-07-15
attributes.tag: growth_protocol
attributes.value: Teosinte lines were selected based upon seed availability and previous research performed by other groups (Flint-Garcia et al., 2009). Teosinte lines were from the Guerrero, Mexico area, approximately 1100 km apart, and had varying seed characteristics. Teosinte lines were grown during the 2014 growing seasons at the South Dakota State University Aurora Research Farm. Soil series was a Brandt silty clay loam (fine-silty, mixed, super-active, frigid Calcic Hapldolls). Additional information regarding the experimental location is available in Clay et al. (2009). Lines were planted May 30, 2014 in an amended four replication split-plot design, with teosinte line being the main treatment, and weedy or non-weedy being the sub-treatment. Plots were fertilized with 140 kg N/hectare with urea treated with urease inhibitor the 1st week of June. Individual treatment plots consisted of a 3.6-m2 area, with 4 seeds (2014) planted in a 3-m x 3-m plot at an equilateral distance from each other and from plot borders. A naturally abundant weed population was allowed to grow unchecked in weedy plots, whereas weed free plots were maintained weed free by hand hoeing and weeding during the growing season.
attributes.tag: treatment
attributes.value: control- non weedy plots
attributes.tag: description
attributes.value: teosinte leaves collected from 3 different weed-free plots
attributes.tag: sub_species
attributes.value: parviglumis
attributes.tag: BioSampleModel
attributes.value: Plant