organism: Bos taurus
description: Protocols: The animal experiment was conducted in according to German Law of Animal Protection and the experimental protocol was approved by the Animal Care Committee of the State Mecklenburg-Western Pomerania, Germany (State Office for Agriculture, Food Safety and Fishery; LALLF M-V/TSD/7221.3-2.1-010/03). At the age of 18 months, the animals were fasted for 24 h and slaughtered in a slaughterhouse facility. Muscle samples (M. longissimus dorsi ) were dissected from the left side of 12th rip area, snap-frozen in liquid nitrogen and kept in -80°C until the RNA extraction. Groupings are as follows: High (59.7 ± 1.0 kg) and low (36.4 ± 0.4 kg) birth weight. Total RNA was isolated using TRI reagent (Sigma-Aldrich). The RNA was treatmented with DNase (Qiagen) and purified using an RNeasy kit (Qiagen) to remove traces genomic DNA. RNA quantity was measured using a Qubit fluorometer (ThermoFisher Scientific) and the integrity was assessed on an Agilent 2100 Bioanalyzer (Agilent Technologies). 2 µg of total RNA with RIN > 7 was used for library preparation using a TruSeq Stranded mRNA Kit with 11 cycles of PCR-amplification according to the manufacture's recommendation (Illumina).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-01-03T15:59:15Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-12-10T14:35:09Z
attributes.tag: External Id
attributes.value: SAMEA5161326
attributes.tag: INSDC center name
attributes.value: Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit
attributes.tag: INSDC first public
attributes.value: 2020-01-03T15:59:15Z
attributes.tag: INSDC last update
attributes.value: 2018-12-10T14:35:09Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7517:M245
attributes.tag: age
attributes.value: 18
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: cattle
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: individual
attributes.value: 245
attributes.tag: organism part
attributes.value: longissimus thoracic muscle
attributes.tag: phenotype
attributes.value: high birth weight
attributes.tag: sample name
attributes.value: E-MTAB-7517:M245
attributes.tag: scientific_name
attributes.value: Bos taurus
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: Charolais x Holstein