organism: Phallusia mammillata
description: Protocols: Sperm was collected from a single adult Phallusia mammillata animal obtained from the Roscoff Marine Station (France). Sperm from a single P. mammillata individual was lysed in 20 ml spooling buffer (75 mM NaCl, 25 mM EDTA pH 8.0, 1% SDS, 0.2mg/ml Proteinase K) at 55 degrees overnight with gentle shaking. 5 ml of saturated NaCl solution were added and the solution was mixed by gentle shaking till homogeneous. 25 ml of isopropanol were added and the solution was mixed by gentle shaking till homogeneous. The stringy DNA precipitate was fished out and washed in 70% ethanol. After removing the 70% ethanol, the DNA pellet was air dried and resuspended in 10mM Tris pH 8 at 55 degrees with gentle shaking. A genomic DNA library was prepared using the NEBNext Ultra II DNA library preparation kit starting with 50 ng of sheared material following the manufacturer's instructions.
attributes.tag: ENA first public
attributes.value: 2020-01-03
attributes.tag: ENA last update
attributes.value: 2018-02-23
attributes.tag: External Id
attributes.value: SAMEA104632935
attributes.tag: INSDC center alias
attributes.value: Cell Biology and Biophysics Unit. European Molecular Biology Laboratory
attributes.tag: INSDC center name
attributes.value: Cell Biology and Biophysics Unit. European Molecular Biology Laboratory
attributes.tag: INSDC first public
attributes.value: 2020-01-03T15:59:08Z
attributes.tag: INSDC last update
attributes.value: 2018-02-23T11:02:54Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6530:Sample 1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: organism part
attributes.value: sperm
attributes.tag: sample name
attributes.value: E-MTAB-6530:Sample 1