organism: freshwater metagenome
description: Microplankton diatoms were collected from station Environment Canada (EC) 341 located in the west-central basin by vertical net tow using a 154 micron mesh size plankton net deployed to 10 to15 meters depth, following which the diatoms were concentrated by gentle filtration on 20 micron pore size polycarbonate membranes and immediately frozen in liquid nitrogen. Total RNA was extracted from the Lake Erie seston using the RNeasy kit ,Qiagen, MA, USA. The RNA sample was sent to the Georgia Genomics Facility (Athens, GA, USA) to generate a cDNA library followed by 454 sequencing using a Genome Sequencer (GS) FLX Titanium instrument (454 Life Sciences, Branford, CT, USA) employing a standard protocol.
attributes.tag: isolation_source
attributes.value: lake erie Epilimnion
attributes.tag: collection_date
attributes.value: 2009-02-17
attributes.tag: geo_loc_name
attributes.value: Canada: Lake Erie EC341
attributes.tag: lat_lon
attributes.value: 41.79833 N 82.29160000 W
attributes.tag: rel_to_oxygen
attributes.value: aerobe
attributes.tag: samp_collect_device
attributes.value: plankton net tow
attributes.tag: samp_mat_process
attributes.value: frozen stored at -80
attributes.tag: samp_size
attributes.value: 5g
attributes.tag: source_material_id
attributes.value: EC_341 Feb. 2009
attributes.tag: BioSampleModel
attributes.value: Metagenome or environmental