organism: Mus musculus
attributes.tag: sample_name
attributes.value: DRS016015
attributes.tag: sample comment
attributes.value: Medaka were bred and growth in a freshwater recirculating aquarium system in the Atmosphere and Ocean Research Institute, the University of Tokyo. The aquarium system was controlled at 26 Â°C with a photoperiod of 14h/10h light/dark cycle. Fish was fed daily with freshly hatched brine shrimp. Individuals (1.0 - 1.2 g body weight) were randomly divided into nine experimental tanks. Medaka were transferred to 50% seawater. In sampling, the experimental fish was anaesthetized in 0.1% tricaine methanesulfonate (MS-222) and further sacrificed by spinal transection. Intestine was immediately dissected, snap-frozen in liquid nitrogen, and stored at -80 Â°C until use. Total RNA was extracted from the intestine samples using Isogen (Nippon Gene, Toyama) according to the manufacturer protocols. Poly(A) RNA was isolated from the total RNA according to the manufacturer protocols.
attributes.tag: Strain
attributes.value: C57BL/6J