organism: Zea mays
description: Protocols: Suitable farms were selected by KALRO extension officers, spaced at least 10km apart, with emphasis placed on getting samples from a wide geographic area. Leaf tissue was detached using clean tweezers, submerged in RNA later (Ambion) and stored on dry ice until a -40 freezer was reached. Sampled maize plants were grown outside, and infected naturally in farmers fields Symptomatic plants were selected from farmers fields under natural infection conditions RNA was extracted using Trizol (Ambion). RNA treated using Ribo-Zero Magnetic Kit (Plant Leaf-Epicentre). Indexed stranded libraries were constructed using Scriptseq V2 RNA-Seq Library Preparation kits and Scriptseq Index PCR primers (Epicentre). Purification steps were performed using Agencourt AMPure XP beads. Library quantity and quality were checked using Qubit (Life Technologies) and on a Bioanalyzer High Sensitivity DNA Chip (Agilent Technologies).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-12-01T17:02:12Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-07-11T10:37:45Z
attributes.tag: External Id
attributes.value: SAMEA4785279
attributes.tag: INSDC center name
attributes.value: Department of Plant Sciences, University of Cambridge
attributes.tag: INSDC first public
attributes.value: 2018-12-01T17:02:12Z
attributes.tag: INSDC last update
attributes.value: 2018-07-11T10:37:45Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7002:T1F7S2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: developmental stage
attributes.value: leaf production stage
attributes.tag: disease
attributes.value: Maize lethal necrosis
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: growth condition
attributes.value: field study
attributes.tag: infect
attributes.value: Maize chlorotic mottle virus, sugarcane mosaic virus
attributes.tag: organism part
attributes.value: leaf
attributes.tag: sample name
attributes.value: E-MTAB-7002:T1F7S2
attributes.tag: sampling site
attributes.value: S00.63207,E036.36880
attributes.tag: scientific_name
attributes.value: Zea mays