organism: Danio rerio
description: Protocols: Total RNAs were extracted from liver tissues of zebrafish larvae. A total amount of 1.5μg RNA per sample was used as input material for the RNA sample preparations. Briefly, mRNA was purified from total RNA using poly-T oligo-attached magnetic beads. Fragmentation was carried out using divalent cations under elevated temperature in NEB. Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer's recommendations and index codes were added to attribute sequences to each sample.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-12-01T17:02:09Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-29T12:32:24Z
attributes.tag: External Id
attributes.value: SAMEA104725948
attributes.tag: INSDC center name
attributes.value: Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College
attributes.tag: INSDC first public
attributes.value: 2018-12-01T17:02:09Z
attributes.tag: INSDC last update
attributes.value: 2018-03-29T12:32:24Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6636:Sample 4
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: zebrafish
attributes.tag: developmental stage
attributes.value: larval stage
attributes.tag: diet
attributes.value: overfeeding
attributes.tag: organism part
attributes.value: liver
attributes.tag: sample name
attributes.value: E-MTAB-6636:Sample 4
attributes.tag: scientific_name
attributes.value: Danio rerio