organism: Saccharomyces cerevisiae
description: Cells were FACS separated. DNA was extracted from the sub-populations and bar-codes for each sub-population were appended to the CDR heavy chains and amplified for Hi-seq sequencing.
attributes.tag: strain
attributes.value: EBY100
attributes.tag: isolate
attributes.value: pRA11B
attributes.tag: host
attributes.value: None
attributes.tag: isolation_source
attributes.value: Synthetic
attributes.tag: collection_date
attributes.value: 2015-08-25
attributes.tag: geo_loc_name
attributes.value: USA: Cold Spring Harbor
attributes.tag: sample_type
attributes.value: cell culture SNP sequencing
attributes.tag: altitude
attributes.value: 0 m
attributes.tag: biomaterial_provider
attributes.value: Justin Kinney at 1 Bungtown Road Simons Center for Quantitative Biology, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA
attributes.tag: collected_by
attributes.value: Rhys Adams
attributes.tag: culture_collection
attributes.value: missing
attributes.tag: depth
attributes.value: not applicable
attributes.tag: env_biome
attributes.value: not applicable
attributes.tag: genotype
attributes.value: pRA11
attributes.tag: host_tissue_sampled
attributes.value: not applicable
attributes.tag: identified_by
attributes.value: Rhys Adams
attributes.tag: lab_host
attributes.value: not applicable
attributes.tag: lat_lon
attributes.value: 40.858312 N 73.465636 W
attributes.tag: mating_type
attributes.value: alpha
attributes.tag: passage_history
attributes.value: 2, FACS
attributes.tag: samp_size
attributes.value: 5 ml
attributes.tag: serotype
attributes.value: pRA11B
attributes.tag: serovar
attributes.value: pRA11B
attributes.tag: specimen_voucher
attributes.value: missing
attributes.tag: temp
attributes.value: 4 C
attributes.tag: BioSampleModel
attributes.value: Microbe, viral or environmental