organism: human gut metagenome
description: Microcosms were constructed using homogenized vegetarian feces from 8 individuals. Microcosm was amended with 10 mM sulfoquinovose and incubated at 37C under anoxic conditions. This metagenome was generated from DNA extracted from biomass, which was harvested 114 h post substrate addition.
attributes.tag: host
attributes.value: Homo sapiens
attributes.tag: isolation_source
attributes.value: 10 mM sulfoquinovose-amended vegetarian human fecal microcosm after 114 h incubation
attributes.tag: collection_date
attributes.value: 2016-11-01
attributes.tag: geo_loc_name
attributes.value: Austria: Vienna
attributes.tag: lat_lon
attributes.value: 48.2084 N 16.3735 E
attributes.tag: rel_to_oxygen
attributes.value: anaerobe
attributes.tag: samp_collect_device
attributes.value: Microcosm (Hungate tubes crimp-sealed with a rubber stopper) was subsampled using an 1 ml sterile syringe and 20G needle
attributes.tag: samp_mat_process
attributes.value: Subsampled fecal biomass was pelleted by centrifugation and pellet was stored at -80C. DNA was harvested by phenol/chloroform extraction.
attributes.tag: samp_size
attributes.value: 1
attributes.tag: env_broad_scale
attributes.value: Vegetarian human fecal microcosm
attributes.tag: env_local_scale
attributes.value: Microcosm amended with 10 mM sulfoquinovose
attributes.tag: env_medium
attributes.value: Fecal slurry in 10 mM bicarbonate buffer
attributes.tag: host_age
attributes.value: 26-55 years
attributes.tag: host_diet
attributes.value: Vegetarian
attributes.tag: host_height
attributes.value: 160-186 cm
attributes.tag: host_sex
attributes.value: pooled male and female
attributes.tag: host_tot_mass
attributes.value: 52-85 kg
attributes.tag: special_diet
attributes.value: vegetarian
attributes.tag: BioSampleModel
attributes.value: Metagenome or environmental