title: IG2
organism: soil metagenome
description: Intensive grassland grazed and periodically (1-5 years) cultivated to grow annual forage crops
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2015-12-31T17:01:14Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-09T12:30:36Z
attributes.tag: External Id
attributes.value: SAMEA3219703
attributes.tag: INSDC center name
attributes.value: INRA-CMSE
attributes.tag: INSDC first public
attributes.value: 2015-12-31T17:01:14Z
attributes.tag: INSDC last update
attributes.value: 2018-03-09T12:30:36Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Level of intensification of land-use
attributes.value: high
attributes.tag: Submitter Id
attributes.value: LTO_18S_IG2
attributes.tag: amount or size of sample collected
attributes.value: 500
attributes.tag: collection date
attributes.value: 2011-11
attributes.tag: current land use
attributes.value: Pasture + annual forage crops
attributes.tag: current vegetation
attributes.value: grassland
attributes.tag: depth
attributes.value: 0.1
attributes.tag: environment (biome)
attributes.value: temperate woodland
attributes.tag: environment (feature)
attributes.value: field soil
attributes.tag: environment (material)
attributes.value: Cambisols
attributes.tag: geographic location (country and/or sea)
attributes.value: Italy
attributes.tag: geographic location (elevation)
attributes.value: 300
attributes.tag: geographic location (latitude)
attributes.value: 40.8162003
attributes.tag: geographic location (longitude)
attributes.value: 9.292335555555555
attributes.tag: investigation type
attributes.value: mimarks-survey
attributes.tag: multiplex identifiers
attributes.value: CGATCGTATA
attributes.tag: pcr conditions
attributes.value: 5ng of DNA were used for a 25µl PCR conducted under the following conditions: initial denaturation: 94degC_3.0min; 35 cycles of denaturation: 94degC_1.0min; annealing: 52degC_1.0min;elongation: 72degC_1.0min;final elongation:72degC_5.0min. A second PCR of 9 cycles was then conducted under similar PCR conditions with purified PCR products and ten base pair multiplex identifiers added to the primers at 5' position to specifically identify each sample and avoid PCR biases.
attributes.tag: pcr primers
attributes.value: FR1 :5-ANCCATTCAATCGGTANT-3 ; FF390 :5-CGATAACGAACGAGACCT-3
attributes.tag: ph
attributes.value: 5.18
attributes.tag: pooling of DNA extracts (ifdone)
attributes.value: yes
attributes.tag: project name
attributes.value: LTO_div_functioning
attributes.tag: sample name
attributes.value: LTO_18S_IG2
attributes.tag: sample weight for DNA extraction
attributes.value: 2
attributes.tag: scientific_name
attributes.value: soil metagenome
attributes.tag: sequencing method
attributes.value: pyrosequencing
attributes.tag: soil environmental package
attributes.value: soil
attributes.tag: storage conditions (fresh/frozen/other)
attributes.value: frozen
attributes.tag: target gene
attributes.value: 18S rRNA gene
attributes.tag: texture
attributes.value: 67/19/14
attributes.tag: total n
attributes.value: 1.5
attributes.tag: total organic carbon
attributes.value: 19.2