title: Sample 29
organism: Mus musculus
description: cloneK8_13-IDE1-d3-1 Protocols: Mouse ESCs were maintained without feeders in LIF+serum medium (DMEM (high glucose, no glutamine, with sodium bicarbonate, Invitrogen) supplemented with 15% ES-qualified EmbryoMax Fetal Calf Serum (Millipore), 10 ng/ml murine LIF (EMBL Protein Expression and Purification Core Facility), 1x Non-Essential Amino Acids, 2 mM L-glutamine, 1 mM sodium pyruvate, 100 U/ml penicillin and 100 microgram/ml strep- tomycin, 0.1 mM 2-mercaptoethanol (all Invitrogen) on culture dishes (Nunc) coated with 0.1% gelatin (Sigma) solution and cultured at 37 degree centigrade with 5% CO2. Medium was changed daily and cells were passaged every other day with 0.05% Trypsin-EDTA (Invitrogen) at a passaging ratio of 1/6- 1/12. mESCs were differentiated to endoderm precursors in Advanced RPMI, 100 U/ml penicillin and 100 microgram/ml streptomycin (both Invitrogen), 0.2% FCS (EmbryoMax, Millipore), 1 microgramM IDE-1 (Tocris) following Borowiak et al. (Cell Stem Cell 2009). Barcoded mRNA libraries were prepared using TruSeq RNA Sample Preparation (Illumina) following the manufacturer's instructions.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2015-12-31T17:01:14Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T22:44:18Z
attributes.tag: External Id
attributes.value: SAMEA3212635
attributes.tag: INSDC center name
attributes.value: Cell Biology and Biophysics Unit. European Molecular BIology Laboratory
attributes.tag: INSDC first public
attributes.value: 2015-12-31T17:01:14Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T22:44:18Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-3234:Sample 29
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: embryonic stem cells
attributes.tag: common name
attributes.value: house mouse
attributes.tag: differentiation day
attributes.value: 3 day
attributes.tag: growth condition
attributes.value: IDE1 endodermal diff
attributes.tag: mir142 genotype
attributes.value: mir142 -/-
attributes.tag: sample name
attributes.value: E-MTAB-3234:Sample 29
attributes.tag: scientific_name
attributes.value: Mus musculus