title: stool-day0-R3
organism: Mus musculus
description: Protocols: oral gavage of helicobacter hepaticus at 1E8 bacterium on days 0,1 and 2 intraperitoneal injection of anti-IL10 receptor antibody at days 0 and 7 For simultaneous DNA and RNA extraction, pellets were dissolved in 500 ul extraction buffer (200 mM NaCl, 20 mM EDTA, 4 M guanidine thiocyanate, 1 % (v/v) I2-mercaptoethanol) and 210 ul 20 % (w/v) SDS and lysed with a bead beater twice for 2 minutes employing Lysing Matrix E tubes (MP Biomedicals). Samples were centrifuged at 8000 g for 5 minutes and the supernatant was diluted with 3 volumes of lysing buffer (ZR-Duet DNA/RNA Miniprep Kit, Zymo Research). DNA and RNA were further extracted employing the ZR-Duet DNA/RNA Miniprep Kit according to the manufacturera's instructions. Libraries were prepared using the PrepX ILM 32i DNA Library Kit Total RNA was depleted of ribosomal RNA using EpibioaTMs universal bacteria ribo-zero kit, single version. Libraries were constructed using the NEBNext Ultra directional RNA Library Prep Kit (E7420L).
attributes.tag: ENA first public
attributes.value: 2015-12-31
attributes.tag: ENA last update
attributes.value: 2015-11-26
attributes.tag: External Id
attributes.value: SAMEA3678200
attributes.tag: INSDC center alias
attributes.value: Kennedy Institute of Rheumatology University of Oxford
attributes.tag: INSDC center name
attributes.value: Kennedy Institute of Rheumatology University of Oxford
attributes.tag: INSDC first public
attributes.value: 2015-12-31T17:01:18Z
attributes.tag: INSDC last update
attributes.value: 2015-11-26T13:44:24Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-4082:stool-day0-R3
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: sample name
attributes.value: E-MTAB-4082:stool-day0-R3
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: C56BL/6