title: B21
organism: Mus musculus
description: Protocols: Mice were euthanized by injection of 250 mg/kg Avertin (20 mg/ml; tribromoethanol) followed by cardiac puncture and exsanguination under deep anesthesia. The brain was dissected and placed in an ice-cold coronal mouse brain matrix. A slab containing the prefrontal cortex (PFC) was removed, and the PFC taken, snap frozen and stored at -80 °C. Mice were co-housed with a single cage mate from either the C57BL6/J or DBA/2J strain from weaning (3 weeks old) to sacrifice (11 weeks old). Total RNA was extracted using a Qiagen Qiacube and RNeasy mini kit according to the manufacturers protocols and was loaded on a 96-well plate, 17 with samples randomly allocated to wells, for shipment to the sequencing centre. Libraries were prepared according to the dUTP strand-specific protocol.
attributes.tag: ENA first public
attributes.value: 2016-12-12
attributes.tag: ENA last update
attributes.value: 2016-12-07
attributes.tag: External Id
attributes.value: SAMEA23684668
attributes.tag: INSDC center alias
attributes.value: EMBL - EBI
attributes.tag: INSDC center name
attributes.value: EMBL - EBI
attributes.tag: INSDC first public
attributes.value: 2016-12-12T11:05:38Z
attributes.tag: INSDC last update
attributes.value: 2016-12-07T16:40:57Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-5276:B21
attributes.tag: age
attributes.value: 11
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cage mate identifier
attributes.value: B44
attributes.tag: common name
attributes.value: house mouse
attributes.tag: environmental history
attributes.value: had C57BL6/J cage mate
attributes.tag: organism part
attributes.value: prefrontal cortex
attributes.tag: sample name
attributes.value: E-MTAB-5276:B21
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: C57BL/6J