title: pcm14 (2)
organism: Drosophila melanogaster
description: Protocols: null pcm mutants pcm14 and pcm15 were induced by excision of P elements from Bloomington stocks 11456 and 33263 respectively. Homo/hemizygous pcm14 and pcm15 mutants are lethal at the pupal stage and were balanced over FM7i-GFP. Homo/hemizygous pcm14 and pcm15 mutants were selected under UV light (non-GFP larvae). wild-type 1 is a clean excision of the same P-element used to create pcm14. wild-type 2 is a clean excision of the same P-element used to create pcm15. 60 L3 wing imaginal discs were dissected per biological replicate for wild-type samples. 120 L3 wing imaginal discs were dissected per replicate for pcm14 and pcm15. RNA was extracted using a Qiagen miRNeasy micro kit (217084) with on-column DNase treatment (79254) Sequencing libraries were created using an Illumina TruSeq RNA Sample Prep Kit v2
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2016-01-01T17:01:22Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-09T02:12:49Z
attributes.tag: External Id
attributes.value: SAMEA3493976
attributes.tag: INSDC center name
attributes.value: Brighton and Sussex Medical School
attributes.tag: INSDC first public
attributes.value: 2016-01-01T17:01:22Z
attributes.tag: INSDC last update
attributes.value: 2018-03-09T02:12:49Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-3789:pcm14 (2)
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: fruit fly
attributes.tag: developmental stage
attributes.value: L3 larva
attributes.tag: genetic modification
attributes.value: pcm null
attributes.tag: genotype
attributes.value: w[1118], pcm[14]/FM7i-GFP
attributes.tag: organism part
attributes.value: wing disc
attributes.tag: sample name
attributes.value: E-MTAB-3789:pcm14 (2)
attributes.tag: scientific_name
attributes.value: Drosophila melanogaster