title: KO3
organism: Plasmodium berghei
description: Protocols: Male Tcr delta-deficient mice (Tcrdtm1Mom) (TCRdelta-/-) and wild-type C57BL/6 mice, of ages 6-8 weeks old, were infected with intravenous (i.v.) inoculation of 2x10^4 GFP-expressing P. berghei ANKA sporozoites purified from mosquito salivary glands. Parasites were collected from the blood at exactly 74 hours after sporozoites infection, at the late trophozoite stage of development. Samples were pooled from 3 mice/ group. DNA was removed with a TURBO DNA-free Kit (Applied Biosystems).​ Barcoded stranded mRNA-seq libraries were prepared using the Illumina TruSeq kit implemented on the liquid handling robot Beckman FXP2.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-04-11T17:04:03Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-03-11T11:36:09Z
attributes.tag: External Id
attributes.value: SAMEA5410848
attributes.tag: INSDC center name
attributes.value: Instituto Gulbenkian de Ciencia, Oeiras, Portugal
attributes.tag: INSDC first public
attributes.value: 2019-04-11T17:04:03Z
attributes.tag: INSDC last update
attributes.value: 2019-03-11T11:36:09Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6493:KO3
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: growth condition
attributes.value: Collected from TCR-/- mice blood
attributes.tag: sample name
attributes.value: E-MTAB-6493:KO3
attributes.tag: scientific_name
attributes.value: Plasmodium berghei
attributes.tag: strain
attributes.value: ANKA