title: nhr-23 GFP L2 C.elegans ChIP Rep1
organism: Caenorhabditis elegans
attributes.tag: source_name
attributes.value: nhr-23 L2 ChIPRep1
attributes.tag: strain
attributes.value: OP43(official name : OP43 genotype : unc-119(ed3); wgIs43(nhr-23::TY1 EGFP FLAG; unc-119) outcross : 3 mutagen : Bombard tags : GFP::3xFlag description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology and Genetics in Dresden using Tony Hyman's recombineering pipeline. The resulting plasmid was used for bombardment transformation of an unc-119(ed3) strain. The NHR-23::EGFP fusion protein is expressed in the correct nhr-23 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the NHR-223 transcription factor. made_by : Bob Waterston's lab from UW )
attributes.tag: developmental stage
attributes.value: L2
attributes.tag: genotype
attributes.value: unc-119(ed3); wgIs43(nhr-23::TY1 EGFP FLAG; unc-119)
attributes.tag: Sex
attributes.value: hermaphrodite