title: Capture_1 from Mycroarray probes on beads
organism: Gossypium hirsutum
description: A total of four regions on a 0.5 454 PTP plate were used to generate sequence capture data for this pilot experiment.  This means, 1/8 of a plate was used to sequence the captured products. Capture 1 was composed entirely of TX2094.  The sample was captured on Mycroarray beads containing probes targeting 534 genes of the cotton exome.  The capture followed the recommended protocols from Mycroarray.  The samples were amplified after capture using the adapter sequences (LM-PCR).  The number of PCR cycles for LM-PCR was empirically determined using a test PCR sample (30 ul) and evaluation of amplificed products of every 5-cycle increment on a Lonza gel.