title: Col-0_rep1
organism: Arabidopsis thaliana
description: Protocols: 13-day-old rosette were cut out and flash frozen in liquid nitrogen Plants were grown in soil in a growth cabinet at 23°C, 50% humidity, using long day conditions (16h light, 8h dark). Total RNA was extracted in TRIzol reagent, precipitated with isopropanol and washed two times in ethanol 70%. RNA was then DNase treated using 1 unit of RQ1 DNAse (Promega) per μg of RNA, following manufacturer's instructions. Following DNase treatment, RNAs were further purified in phenol-chloroform. RNA integrity was assessed by running 1ug of RNA through a agarose gel supplemented with formaldehyde after denaturation in 1X MOPS 4% formaldehyde for 15 minutes at 65°C. Sequencing libraries were generated at Fasteris S.A. (Geneva, Switzerland).
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism
attributes.value: Arabidopsis thaliana
attributes.tag: age
attributes.value: 18
attributes.tag: developmental stage
attributes.value: mid rosette growth stage
attributes.tag: organism part
attributes.value: rosette
attributes.tag: genotype
attributes.value: Col-0
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-05-19
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-04-14
attributes.tag: ecotype
attributes.value: Col-0