title: NHR-21 GFP L2 C.elegans ChIP Rep1
organism: Caenorhabditis elegans
attributes.tag: source_name
attributes.value: NHR-21 L2 ChIPRep1
attributes.tag: strain
attributes.value: OP361(official name : OP361 genotype : unc119(ed3); wgIs361(nhr-21::TY1-GFP-3xFLAG; unc119) outcross : 3 mutagen : Bombard tags : GFP::3xFlag description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Tubiginen using Tony Hyman's recombineering pipeline. The resulting plasmid was used for biolistic transformation of an unc-119(ed3) strain. The NHR-21::EGFP fusion protein is expressed in the correct nhr-21 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the NHR-21 transcription factor. made_by : Unknown )
attributes.tag: developmental stage
attributes.value: L2
attributes.tag: genotype
attributes.value: unc119(ed3);wgIs361(nhr-21::TY1-GFP-3xFLAG;unc119)
attributes.tag: Sex
attributes.value: hermaphrodite