title: sRNAs from SINV-infected transgenic flies expressing RFP tagged hDicer in a Dicer-2 null background, 5 dpi
organism: Drosophila melanogaster
attributes.tag: source_name
attributes.value: Transgenic flies with a Dicer-2 null background
attributes.tag: cell line
attributes.value: None
attributes.tag: construct
attributes.value: RFP::hDicer
attributes.tag: growth protocol
attributes.value: Flies were fed on standard cornmeal-agar medium at 25°C, tested for Wolbachia infection and cured whenever necessary.
attributes.tag: viral infection condition
attributes.value: 5 days post-infection by intrathoracic injection with viral particles prepared in 10 mM Tris-HCL, pH 7.5 (SINV 5x10^8 PFU/ml)
attributes.tag: extract protocol
attributes.value: Total RNA was extracted from infected flies using TRI Reagent RT bromoanisole solution (MRC)