title: MR33
organism: Homo sapiens
description: Protocols: The hESCs H9 (WA-09)-NURR1-GFP reporter line was maintained using E8-essential medium (Fisher Scientific) without feeder on VTA-N (Fisher Scientific) and passaged every 4-5 days by EDTA. Midbrain dopamine (mDA) differentiation from hESC was done with an optimized protocol from previously published by our group. mock (solvent) or 50uM 6OHDA treatment for 16h ChIP was performed and DNA was purified with a magnetic bead-based purification method according to the manufacturer's protocol (MAGnify Chromatin Immunoprecipitation System-Kit, LifeTechnologies). sheared DNA was used to generate ChIP-sequencing libraries by employing Ovation Ultralow System V2 Kit (NuGEN).
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism
attributes.value: Homo sapiens
attributes.tag: cell_line
attributes.value: H9
attributes.tag: progenitor cell type
attributes.value: embryonic stem cell
attributes.tag: cell_type
attributes.value: dopaminergic neuron
attributes.tag: genotype
attributes.value: NURR1::GFP
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-07-22
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2017-08-15