title: Medicago truncatula cv. Jemalong A17 S. Meliloti Inoculated CPTIO treated root sample A
organism: Medicago truncatula
description: Medicago truncatula roots 4 days after inoculation with S. meliloti strains grown in LB medium supplemented with 2.5 mM CaCl2 and 2.5 mM MgSO4 (LBMC). Plants were treated with 2-(4-carboxyphenyl)-4,4,5,5-tetramethyl imidazoline-1-oxyl-3-oxide (cPTIO; Sigma), 250 *l of a 1 mM solution prepared in tris-KCl 10mM buffer was added along the whole length of the roots. The plants were allowed to stay in contact with cPTIO for 8 hours.