title: Sample 5
organism: Homo sapiens
description: Protocols: Total RNA was isolated from hMLOs using TRIzol Reagent (Thermo Fisher). DNA contaminants were digested with DNASE I (Ambion). For RNA-seq library preparation, total RNA was further purified using the Purelink RNA Mini kit (Ambion). 1.5 ug total RNA was used for library construction according to manufacturer's protocol (TruSeq stranded mRNA Library Preparation Kit, Illumina).
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism
attributes.value: Homo sapiens
attributes.tag: cell_line
attributes.value: H9
attributes.tag: developmental stage
attributes.value: embryo
attributes.tag: organism part
attributes.value: inner cell mass
attributes.tag: progenitor cell type
attributes.value: embryonic stem cell
attributes.tag: genotype
attributes.value: GBA1 knockout
attributes.tag: growth condition
attributes.value: midbrain-like organoids
attributes.tag: sampling time point
attributes.value: 90
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-10-01
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-10-17