title: 16S rRNA gene fragments from Monju chimney in Kairei field.
organism: uncultured marine microorganism
attributes.tag: sample_name
attributes.value: DRS001478
attributes.tag: sample comment
attributes.value: Monju site was located at north of Fugen site (25Â°19.2200'S, 70Â°02.4066'E) and we found active black smoker chimneys in this site. The vent fluid temperature was 299Â°C. We sampled the chimney. We extracted DNA and subjected to PCR amplification. The amplified 100 bp of 16S rRNA gene fragments were used for 454 pyrosequencing analysis.