title: 04_d1_mid_T_rep1
organism: Mus musculus
description: Protocols: T(Brachyury)-inducible OCT4-YFP ESCs cultured in 2iLIF system were stimulated by doxycycline and dexamethasone for 24 hr in order to induce T. Different dose fractions of the T-induced cells were sorted and collected for the following RNA-seq experiment by using the fluorescence-Activated Cell Sorting (FACS) Analysis. Total RNA was isolated using a NucleoSpin RNA Mini kit (MACHEREY-NAGEL) and then, mRNA was isolated using NEB Next Poly A m-RNA magnetic bead selection kit (E7490S). RNA-seq library was prepared using NEBNext Ultra II RNA Library Prep Kit for Illumina (E7770).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-09-17T04:04:25Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-08-27T15:23:10Z
attributes.tag: External Id
attributes.value: SAMEA7279324
attributes.tag: INSDC center name
attributes.value: Max Planck Institute for Molecular Biomedicine, Department Cell and Developmental Biology
attributes.tag: INSDC first public
attributes.value: 2020-09-17T04:04:25Z
attributes.tag: INSDC last update
attributes.value: 2020-08-27T15:23:10Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-9494:04_d1_mid_T_rep1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: OCT4-YFP embryonic stem cells
attributes.tag: cell type
attributes.value: embryonic stem cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: embryo stage
attributes.tag: genotype
attributes.value: OCT4-YFP transgenic reporter
attributes.tag: sample name
attributes.value: E-MTAB-9494:04_d1_mid_T_rep1
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: stimulus
attributes.value: mid T-induction