title: june_10_488m_B
organism: phyllosphere metagenome
description: Phyllosphere fungal assemblages associated with 15 beech leaves taken from 5 trees
attributes.tag: plant body site
attributes.value: adult vascular leaf PO:0006340
attributes.tag: Investigation type
attributes.value: MIMARKS
attributes.tag: Project name
attributes.value: gradient_pyrenees
attributes.tag: Enviornmental package
attributes.value: host-associated
attributes.tag: organism
attributes.value: phyllosphere metagenome
attributes.tag: target gene
attributes.value: ITS1-ITS2
attributes.tag: Environment (biome)
attributes.value: Temperate broadleaf and mixed forest biome ENVO:00000876
attributes.tag: host taxid
attributes.value: 28930
attributes.tag: scientific_name
attributes.value: phyllosphere metagenome
attributes.tag: Environment (material)
attributes.value: phyllosphere
attributes.tag: ENA-KEYWORD
attributes.value: GSC, MIxS, MIMARKS
attributes.tag: host common name
attributes.value: European beech
attributes.tag: pcr primers
attributes.value: ITS2 : CGTATCGCCTCCCTCGCGCCATCAGATGCGGCTGCGTTCTTCATCGATGC ITS1F : CTATGCGCCTTGCCAGCCCGCTCAGATCGCCTTGGTCATTTAGAGGAAGTAA
attributes.tag: Geographic location (longitude)
attributes.value: -0.08722222
attributes.tag: Geographic location (latitude)
attributes.value: 43.09611111
attributes.tag: pcr conditions
attributes.value: initial denaturation:95degC_5min; 30 cycles of denaturation:95degC_30sec; annealing=54degC_1min; extension=72degC_1min30sec; final extension=72degC_10min
attributes.tag: Elevation
attributes.value: 488
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2011-12-06T11:09:11Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-09T09:55:08Z
attributes.tag: Sequencing method
attributes.value: Pyrosequencing
attributes.tag: Collection date
attributes.value: 3rd June 2010
attributes.tag: Geographic location (country:region,area)
attributes.value: France
attributes.tag: multiplex identifiers
attributes.value: ATGCG
attributes.tag: Environment (feature)
attributes.value: plant-associated habitat ENVO:00009001
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2011-12-06
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-09