title: TA070
organism: Homo sapiens
description: Protocols: Cells were isolated on October 14th 2014 from two samples of healthy human donor blood of unknown gender via LSM1077 gradient centrifugation. Adherent cells were positivly selected on primaria 6 well dishes (3x10^6 monocytes per well in 2 ml PBS (PAA)) followed by 6 washing steps with PBS. Finally the monocytes were cultured for 7d in humidified incubator at 37 °C, 5 % CO2 in 2ml RPMI medium supplemented with 10 % FCS (both PAA). Lysis in hypotonic lysis buffer Nuclei were resuspended in ChIP RIPA and sheared with a Branson S250D using a microtip (60 pulses of 1 s, 2 s pause, 20% amplitude) with coolling (ice-ethanol). A 15 min 20,000xg supernatant was used for IP (after preclearing). The Diagenode Microplex Library Preparation kit (Diagenode, C05010011) was used according to the manufacturer's instructions with 1-2 ng of input DNA. DNA concentrations were measured on a Nanodrop 3300 using the Quant-It dsDNA High-Sensitivity Assay Kit (Life Technologies).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2015-12-31T17:01:14Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T21:48:55Z
attributes.tag: External Id
attributes.value: SAMEA3139809
attributes.tag: INSDC center name
attributes.value: Institute for molecular and tumor biology Philipps University Marburg Germany
attributes.tag: INSDC first public
attributes.value: 2015-12-31T17:01:14Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T21:48:55Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-3113:TA070
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: macrophage
attributes.tag: common name
attributes.value: human
attributes.tag: sample name
attributes.value: E-MTAB-3113:TA070
attributes.tag: scientific_name
attributes.value: Homo sapiens