title: Adult Dirofilaria immitis
organism: Dirofilaria immitis
description: Live adult heartworms were collected at necropsy of an adult dog with sudden death, obtained from a pet hospital in Ya’an, Sichuan, China. Phosphate-buffered saline (PBS; pH 7.4; 37℃) were used to clean the live worms, both males and females, for five times to remove contamination from the host. Then the worms were immediately frozen in liquid nitrogen and stored at -80℃ until use.