title: CF4-B sputum from Cystic Fibrosis individual
organism: human metagenome
description: CF4-B Microbial Metatranscriptomic. Sputum was collected from CF154 patient at time point D (02/01/2011). Detailed experimental design is published in Lim et al. 2011. Sample was split into three parts, (i) Ambion MICROBEnrich and MICROExpress kit microbial mRNA enrichment, (ii) Ribo-Zero Meta Kit human and microbial rRNA removal, and (iii) no rRNA removal. cDNA was generated using the Whole Transcriptome Amplification kit (WTA2).
attributes.tag: Keywords
attributes.value: GSC:MIMS
attributes.tag: host
attributes.value: human
attributes.tag: material
attributes.value: sputum
attributes.tag: env_package
attributes.value: human-associated
attributes.tag: host_taxid
attributes.value: 9606
attributes.tag: body_site
attributes.value: lung
attributes.tag: feature
attributes.value: human-associated habitat; ENVO:00009003
attributes.tag: disease_stat
attributes.value: Cystic Fibrosis
attributes.tag: biome
attributes.value: terrestial biome