title: grxs17-1_R1
organism: Arabidopsis thaliana
description: Protocols: Seeds were surface-sterilized and sown in MS-agar medium and stratified at 4°C in darkness for 3 days, then germinated for one week under long days (16h light/8h dark) at 21°C. RNeasy Plant Mini Kit (Qiagen) and DNase I treated (Promega) preparation of strand-specific cDNA libraries: Purification of poly-A containing mRNA molecules; mRNA fragmentation; Random primed cDNA synthesis (strand specific); Adapter ligation and adapter specific PCR amplification.
attributes.tag: ENA first public
attributes.value: 2018-01-02
attributes.tag: ENA last update
attributes.value: 2016-08-10
attributes.tag: External Id
attributes.value: SAMEA4378188
attributes.tag: INSDC center alias
attributes.value: Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, Belgium Department of Plant Systems Biology,VIB, Gent, Belgium
attributes.tag: INSDC center name
attributes.value: Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, Belgium Department of Plant Systems Biology,VIB, Gent, Belgium
attributes.tag: INSDC first public
attributes.value: 2018-01-02T17:02:30Z
attributes.tag: INSDC last update
attributes.value: 2016-08-10T17:21:10Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-5001:grxs17-1_R1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: thale cress
attributes.tag: developmental stage
attributes.value: Seedling
attributes.tag: ecotype
attributes.value: Columbia
attributes.tag: genetic modification
attributes.value: SALK_021301
attributes.tag: replicate
attributes.value: R1
attributes.tag: sample name
attributes.value: E-MTAB-5001:grxs17-1_R1