title: OT-control-002-L
organism: Agaricus bisporus
description: commercially purchased button mushroom as positive control, Long amplicon
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-05-18T04:06:29Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-03-20T12:31:28Z
attributes.tag: External Id
attributes.value: SAMEA6633765
attributes.tag: INSDC center name
attributes.value: UPPSALA UNIVERISTY
attributes.tag: INSDC first public
attributes.value: 2020-05-18T04:06:29Z
attributes.tag: INSDC last update
attributes.value: 2020-03-20T12:31:28Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: OT-control-002-L
attributes.tag: common name
attributes.value: common button mushroom
attributes.tag: geographic location (country and/or sea)
attributes.value: Sweden
attributes.tag: geographic location (region and locality)
attributes.value: Uppsala
attributes.tag: isolation source
attributes.value: purchased at local grocery store
attributes.tag: pcr conditions
attributes.value: initial denaturation:95degC_10min; denaturation:95degC_45s; annealing:59degC_45s; extension:72degC_90s; cycles:30; final extension:72degC_10min
attributes.tag: pcr primers
attributes.value: ITS1-0007_For: GGTAGACAGTCTATACTGCTGTCCGTAGGTGAACCTGC; LR5-0016_Rev: GGTAGTAGATGCGAGAGTAGATCCTGAGGGAAACTTCG
attributes.tag: project name
attributes.value: ena-STUDY-UPPSALA UNIVERISTY-19-03-2020-10:28:50:771-6
attributes.tag: sample name
attributes.value: OT-control-002-L
attributes.tag: scientific_name
attributes.value: Agaricus bisporus
attributes.tag: sequencing method
attributes.value: PACBIO_SMRT
attributes.tag: target gene
attributes.value: ITS1, 5.8S, ITS2, LSU