title: Control DNA sequences for analysis of allelic imbalance in D. melanogaster - D. simulans hybrid head tissues
organism: Drosophila
description: Isogenic strains of D. simulans (C167.4; BDSC 4736) and D. melanogaster (Berlin; BDSC 8522) were crossed to produce F1 hybrid progeny. One to one and a half day old adults were flash frozen in synchronized batches.DNA was extracted from bodies of a single pool of 20 individuals. DNA was extracted using the MasterPure DNA purification kit (Epicentre). The Genomic DNA Clean and Concentrator kit (Zymo Research) was used to isolate only high molecular weight DNA, following the manufacturer’s protocol.