title: Central starchy endosperm 3
organism: Triticum aestivum
description: Protocols: Heads were tagged at anthesis and pure starchy endosperm dissected at 10, 14, 17, 21, and 28dpa for transcript analysis. The outside two florets on the central four spikelets on each side of the ear, in total 16 caryopses, were dissected to yield pure central starchy endosperm of the developing grain. Each head was considered a biological replicate for the qRT analysis, and up to 10 replicates were pooled for the RNA sequencing and biochemical analysis. Dissected samples were immediately frozen in liquid nitrogen, lyophilised, and homogenised in a TissueLizer (Qiagen). Bread wheat (Triticum aestivum) cv Cadenza plants were grown in controlled environment as described in Sparks and Jones, 2009, (In HD Jones, PR Shewry, eds, Transgenic Wheat, Barley and Oats. Humana Press, pp 71-92).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2011-11-26T17:00:49Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T15:33:31Z
attributes.tag: External Id
attributes.value: SAMEA1094612
attributes.tag: INSDC center name
attributes.value: GATC Biotech AG
attributes.tag: INSDC first public
attributes.value: 2011-11-26T17:00:49Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T15:33:31Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-861:Central starchy endosperm 3
attributes.tag: age
attributes.value: 17 d
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: bread wheat
attributes.tag: initial time point
attributes.value: anthesis
attributes.tag: organism part
attributes.value: starchy endosperm
attributes.tag: sample name
attributes.value: E-MTAB-861:Central starchy endosperm 3
attributes.tag: scientific_name
attributes.value: Triticum aestivum