title: OT-blank-002-S
organism: blank sample
description: PCR blank
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-05-18T04:06:30Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-04-20T14:20:02Z
attributes.tag: External Id
attributes.value: SAMEA6792970
attributes.tag: INSDC center name
attributes.value: UPPSALA UNIVERISTY
attributes.tag: INSDC first public
attributes.value: 2020-05-18T04:06:30Z
attributes.tag: INSDC last update
attributes.value: 2020-04-20T14:20:02Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: OT-blank-002-S
attributes.tag: common name
attributes.value: blank sample
attributes.tag: geographic location (country and/or sea)
attributes.value: Sweden
attributes.tag: geographic location (region and locality)
attributes.value: Uppsala
attributes.tag: isolation source
attributes.value: Nuclease free water
attributes.tag: pcr conditions
attributes.value: initial denaturation:95degC_10min; denaturation:95degC_60s; annealing:56degC_45s; extension:72degC_50s; cycles:35; final extension:72degC_3min
attributes.tag: pcr primers
attributes.value: gITS7mod-35: CCATCTCATCCCTGCGTGTCTCCGACTCAGTAAGCCATTGTCGATGTGARTCATCGARTCTTTG; ITS4: TCCTCCGCTTATTGATATGC, ITS4A: TCCTCGCCTTATTGATATGC
attributes.tag: project name
attributes.value: ena-STUDY-UPPSALA UNIVERISTY-19-03-2020-10:28:50:771-6
attributes.tag: sample name
attributes.value: OT-blank-002-S
attributes.tag: scientific_name
attributes.value: blank sample
attributes.tag: sequencing method
attributes.value: ION_TORRENT, PACBIO_SMRT, ILLUMINA
attributes.tag: target gene
attributes.value: ITS2