title: BW39
organism: Mus musculus
description: Protocols: At 4 weeks mice were anesthetized using isoflurane and blood was collected by retro-orbital bleeding, plasma was prepared and immediately stored at -80ºC for later assessment of metabolites. Livers were collected and immediately freeze-clamped with tongs cooled in liquid nitrogen and stored at -80°C until used to measure metabolites, DNA and RNA or stored in 10% neutral buffered formalin. All procedures were performed according to protocols approved by the Animal Care and Use Committee of the National Institutes of Health. The Ovation RRBS Methyl-Seq system (NuGen) was used to generate the library and the EpiTect Fast DNA Bisulfite Kit (Qiagen) was used for bisulfite conversion. The library quality and quantity was determined with a Qubit HS (Invitrogen, Q32854) and Agilent Bioanalyzer DNA 1,000 kit followed by post processing Kapa RT-PCR (KK4873).
attributes.tag: ENA first public
attributes.value: 2020-09-18
attributes.tag: ENA last update
attributes.value: 2016-10-26
attributes.tag: External Id
attributes.value: SAMEA4523709
attributes.tag: INSDC center alias
attributes.value: University of North Carolina at Chapel Hill
attributes.tag: INSDC center name
attributes.value: University of North Carolina at Chapel Hill
attributes.tag: INSDC first public
attributes.value: 2020-09-18T04:05:58Z
attributes.tag: INSDC last update
attributes.value: 2016-10-26T12:31:32Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-5204:BW39
attributes.tag: age
attributes.value: 4
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: liver
attributes.tag: sample name
attributes.value: E-MTAB-5204:BW39
attributes.tag: strain
attributes.value: C57BL/6J