title: Acellular_K/BxN_thoracic_lymph
organism: Mus musculus
description: Protocols: Thoracic lymph was collected as described in Milasan, Tessandier et al, JEV, 2016 (PMID: 27664155). Cells were removed by centrifugation (400 g, 5 min), and acellular lymph was stored at -80C up to RNA extraction. RNA was extracted using standard TriZol LS protocol. Total RNA of each sample was used to prepare the miRNA sequencing library which included the following steps: 1) 3'-adapter ligation with T4 RNA ligase 2 (truncated); 2) 5'-adapter ligation with T4 RNA ligase; 3) cDNA synthesis with RT primer; 4) PCR amplification; 5) extraction and purification of ~130-150 bp PCR amplified fragments (correspond to ~16-30 nt small RNAs) from the PAGE gel.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-10-01T04:02:54Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-07-23T15:34:24Z
attributes.tag: External Id
attributes.value: SAMEA5806235
attributes.tag: INSDC center name
attributes.value: Centre de Recherche du Centre Hospitalier Universitaire de Quebec
attributes.tag: INSDC first public
attributes.value: 2019-10-01T04:02:54Z
attributes.tag: INSDC last update
attributes.value: 2019-07-23T15:34:24Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8171:Acellular_K/BxN_thoracic_lymph
attributes.tag: age
attributes.value: 8 to 9
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: disease
attributes.value: rheumatoid arthritis
attributes.tag: genotype
attributes.value: FcgRIIA TGN +/+
attributes.tag: individual
attributes.value: pooled from 3 mice
attributes.tag: organism part
attributes.value: lymph
attributes.tag: sample name
attributes.value: E-MTAB-8171:Acellular_K/BxN_thoracic_lymph
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: C57BL/6