title: Jack pine bark and xylem from leader and internodes of a mechanically wounded and methyl jasmonate sprayed tree
organism: Pinus banksiana
description: Jack pine (P. banksiana clone ID PSB 410 1 0) saplings were maintained outdoors at the University of British Columbia (Hall et al., 2011).  For RNA extractions, four 2 year old saplings of each species were moved into the greenhouse at the University of British Columbia and were maintained at 24C and 16 hr light per day for 2 weeks prior to induction of defense gene expression.  For induction, the stem of each tree was wounded with a razor blade and the aerial portion of the tree was sprayed with 50 ml of 0.1% methyl jasmonate.  One quarter of the stem (combined bark and xylem) of each tree was harvested 2h, 6h, 24h and 48 h following treatment and the four time points from each individual were pooled prior to RNA extraction).