title: S_ve_ctrl_rep1_run1
organism: Zea mays
description: Protocols: Samples were taken at three growth stages: coleoptile (VE); seedling with the tip of the second leaf visible (called here “VE/V1 stage”); seedling with the first leaf fully developed (V1, ligular region present). Each sample consisted of pooled material from at least three plants (coleoptile for VE, first leaf for VE/V1 and V1 stages). Plants were grown from kernels in a growth chamber under 14h/10h photoperiod, light irradiance of 500 μmol quanta m−2 s−1 during the light phase, and 70% air relative humidity. Day/night temperature 24°C/20°C from the start of the experiment. Nucleosome-free DNA was extracted according to the FAIRE procedure. Briefly, leaf samples were fixed with formaldehyde solution, crosslinked chromatin was sonicated (to 200-800 bp range), isolated with Phenol-Chlorophorm-Isoamyl alcohol method and cleaned with DNA Clean & Concentrator-5 (Zymo Research, USA). Standard Protocol Illumina ChIP-Seq TruSeq
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-09-18T04:04:32Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-10-02T17:49:29Z
attributes.tag: External Id
attributes.value: SAMEA5988927
attributes.tag: INSDC center name
attributes.value: Faculty of Biology, University of Warsaw
attributes.tag: INSDC first public
attributes.value: 2020-09-18T04:04:32Z
attributes.tag: INSDC last update
attributes.value: 2019-10-02T17:49:29Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8401:S_ve_ctrl_rep1_run1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cultivar
attributes.value: S68911
attributes.tag: developmental stage
attributes.value: coleoptile emergence stage
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: coleoptile
attributes.tag: sample name
attributes.value: E-MTAB-8401:S_ve_ctrl_rep1_run1
attributes.tag: scientific_name
attributes.value: Zea mays