title: ELT-3 GFP EMB C.elegans Input Rep2
organism: Caenorhabditis elegans
attributes.tag: source_name
attributes.value: ELT-3 EMB InputRep2
attributes.tag: strain
attributes.value: OP75(official name : OP75 genotype : unc-119 (ed3) III; wgIs75 [elt-3::TY1::EGFP::FLAG; unc-119 (+)] outcross : 3 mutagen : Bombard tags : GFP::3xFlag description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Tubiginen using Tony Hyman's recombineering pipeline. The resulting plasmid was used for biolistic transformation of an unc-119(ed3) strain. The ELT-3::EGFP fusion protein is expressed in the correct elt-3 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the ELT-3 transcription factor. made_by : R Waterston )
attributes.tag: developmental stage
attributes.value: embryo
attributes.tag: genotype
attributes.value: unc-119 (ed3) III; wgIs75 [elt-3::TY1::EGFP::FLAG; unc-119 (+)]
attributes.tag: sex type
attributes.value: mixed Male and Hermaphrodite population