title: MNase sequencing of macronuclei from vegetative Paramecium tetraurelia (MonoNuc 3-2 replicate)
organism: Paramecium tetraurelia
description: Mono-nucleosomal DNA fragments were obtained by MNase digestion of chromatin isolated from macronuclei of exponentially growing vegetative P. tetraurelia (strain 51 mating type 7) cells for 2 biological replicates (PTET_WT_VEG_MNASE_SEQ_MonoNuc_3-2 and PTET_WT_VEG_MNASE_SEQ_MonoNuc_4-1). As controls, two DNA samples extracted from macronuclei of exponentially growing vegetative P. tetraurelia (strain 51 mating type 7) were digested with MNase and DNA fragments of the same molecular weight as the mono-nucleosomal DNA fragments were selected for library preparation (PTET_WT_VEG_MNASE_SEQ_NakedDNA_D1 and PTET_WT_VEG_MNASE_SEQ_NakedDNA_D2). Paired-end Illumina sequencing was performed to assess the genome-wide nucleosome profiles.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-12-15T17:12:12Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-09-11T11:29:48Z
attributes.tag: External Id
attributes.value: SAMEA7296307
attributes.tag: INSDC center name
attributes.value: INSTITUT JACQUES MONOD
attributes.tag: INSDC first public
attributes.value: 2020-12-15T17:12:12Z
attributes.tag: INSDC last update
attributes.value: 2020-09-11T11:29:48Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: PTET_WT_VEG_MNASE_SEQ_MonoNuc_3-2
attributes.tag: sample name
attributes.value: PTET_WT_VEG_MNASE_SEQ_MonoNuc_3-2
attributes.tag: scientific_name
attributes.value: Paramecium tetraurelia
attributes.tag: strain
attributes.value: 51