organism: Bos taurus
description: DNAmixture of 44 semens - The sample was the mixture of DNA extracted from 44 Chinese Holstein bulls semen
attributes.tag: Common Name
attributes.value: cattle
attributes.tag: Sample Name
attributes.value: pooled DNA
attributes.tag: Scientific Name
attributes.value: Bos taurus
attributes.tag: Sex
attributes.value: male
attributes.tag: Submission description
attributes.value: We designed and synthesized 23 pairs of primers in the entire exons, and 3 kb of 5′ and 3′ flanking regions of bovine LIPJ gene according to the genomic sequences of (GenBank accession number: NC_037353.1). 44 semen DNAs were pooled to use for the PCR amplifications with the equal concentration (50 ng/µL) for each DNA. All the PCR products were sequenced by ABI3730XL DNA analyzer (Applied Biosystems, Foster, CA, USA). Then, we aligned the sequences with the reference genome (UMD3.1.1) using the BLAST (https://blast.ncbi.nlm.nih.gov/Blast.cgi), and identified the novel SNP, g.10250120A/G.
attributes.tag: Submission identifier
attributes.value: GSB-702
attributes.tag: Submission title
attributes.value: the National Science and Technology Programs of China
attributes.tag: collected_by
attributes.value: Dongxiao Sun