title: Sample 1
organism: Homo sapiens
description: Protocols: Methodology: U2OS cells were infected with retrovirus expressing NUAK1 shRNA4977 (see Liu et al. Nature. 2012 for shRNA details) or non-targeting control shRNA. 24hrs after infected, cells were selected on Puromycin for 48hrs, then trypsinized and re-seeded. Cells were harvested 24hrs after re-seeding and RNA was isolated using Qiagen RNEasy midi kits. cDNA was generated using TruSeq Stranded mRNA Library Prep Kit (Illumina). Following library quantification (D1000 ScreenTape, Agilent Technologies), libraries were standardized to 10nM, denatured, diluted to 10pM and analyzed by paired-end sequencing using an Illumina NextSeq500 platform. Sample number NM 1 to 3 are treated with shCtrl. Sample number NM7 to 9 are shNUAK1. Cells were lysed directly on 10cm dishes using Qiagen RNEasy extraction buffer RNA was isolated using Qiagen RNEasy cDNA was synthesized with the TruSeq Stranded mRNA Library Prep Kit (Illumina)
attributes.tag: ENA first public
attributes.value: 2018-01-01
attributes.tag: ENA last update
attributes.value: 2017-11-24
attributes.tag: External Id
attributes.value: SAMEA104402382
attributes.tag: INSDC center alias
attributes.value: BAH-GU
attributes.tag: INSDC center name
attributes.value: University of Glasgow
attributes.tag: INSDC first public
attributes.value: 2018-01-01T17:01:44Z
attributes.tag: INSDC last update
attributes.value: 2017-11-24T13:53:16Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6244:Sample 1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: U2OS
attributes.tag: cell type
attributes.value: epithelial cell
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: osteosarcoma
attributes.tag: organism part
attributes.value: bone tissue
attributes.tag: sample name
attributes.value: E-MTAB-6244:Sample 1