title: 22334_8_185
organism: Homo sapiens
description: Protocols: Cells were grown in DMEM, high glucose, GlutaMAX Supplement, pyruvate (Life Technologies / 10569-010) + Fetal Bovine Serum (Lab Tech / FB-1001) + Penicillin - Streptomycin (Life Technologies / 15140122). All cells were derived from healthy donors and were nontransgenic. Reverse transcription and cDNA amplification was performed according to the SmartSeq2 protocol (Picelli et al., 2014) Library preparation was performed using an Illumina Nextera kit. We used ERCC spike ins from Life Technologies, catalogue number 4456740, at a concentration of 1:64Million. All uploaded cells have passed our QC (we have not included failed cells), we have outlined the QC process/thresholds in the accompanying publication.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-09-30T04:05:10Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-09-20T14:25:31Z
attributes.tag: External Id
attributes.value: SAMEA4933338
attributes.tag: INSDC center name
attributes.value: EMBL-EBI & Wellcome Sanger Institute
attributes.tag: INSDC first public
attributes.value: 2019-09-30T04:05:10Z
attributes.tag: INSDC last update
attributes.value: 2018-09-20T14:25:31Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-71671537448367:22334_8_185
attributes.tag: age
attributes.value: 67
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: fibroblast of dermis
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: normal
attributes.tag: individual
attributes.value: vuna
attributes.tag: organism part
attributes.value: skin
attributes.tag: post analysis well quality
attributes.value: pass
attributes.tag: sample name
attributes.value: E-MTAB-71671537448367:22334_8_185
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: female
attributes.tag: single cell quality
attributes.value: OK
attributes.tag: single cell well quality
attributes.value: OK