title: C2C12_G_accessibility
organism: Mus musculus
attributes.tag: sample_name
attributes.value: DRS001050
attributes.tag: sample comment
attributes.value: Restriction enzyme accessibility experiments using a ligation-mediated-PCR protocol were performed as described previously (de la Serna et al., 2005). Briefly, nuclei were digested with PvuII and the genomic DNA was purified using a DNeasy Blood & Tissue kit (Qiagen).DNA flagments were were extracted by Long Mate-Paired Library kit (Life Technologies).The purified library was used for cluster generation and sequencing analysis using the Genome Analyzer GAIIx (Illumina K. K.).