title: HRASC
organism: Danio rerio
description: Protocols: 8 dpf wildtype and Tg(twhh:Gal4);Tg(UAS:EGFP-HRASV12) were euthanized with 3% Tricaine methanesulfonate (Sigma). Embryos were decapitated and incubated in Tripsin-EDTA (Sigma) for 30 minutes to facilitate tissue dissociation. Notochords were then dissected using tungsten needles and immediately transferred to Trizol-LS (Ambion). We isolated 30-50 notochords per replicate, with a total of 3 replicates per condition (3x wildtype, 3x HRASV12). Notochord RNA was extracted following the manufacturer's protocol using Trizol-LS. RNA-seq libraries were constructed using the Truseq stranded total RNA kit.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-12-01T17:03:21Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-10-30T13:30:13Z
attributes.tag: External Id
attributes.value: SAMEA5055149
attributes.tag: INSDC center name
attributes.value: UZH
attributes.tag: INSDC first public
attributes.value: 2018-12-01T17:03:21Z
attributes.tag: INSDC last update
attributes.value: 2018-10-30T13:30:13Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7349:HRASC
attributes.tag: age
attributes.value: 8
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: zebrafish
attributes.tag: developmental stage
attributes.value: embryo
attributes.tag: genotype
attributes.value: Tg(twhh:Gal4);Tg(UAS:EGFP-HRASV12)
attributes.tag: individual
attributes.value: mixed pool of 30 to 50 embryos
attributes.tag: organism part
attributes.value: notochord
attributes.tag: sample name
attributes.value: E-MTAB-7349:HRASC
attributes.tag: scientific_name
attributes.value: Danio rerio
attributes.tag: sex
attributes.value: mixed