title: IR29-RBOHB-SSR
organism: Oryza sativa Indica Group
description: Protocols: A leaf punch of approximately 1 centimeter in diameter was collected from plants DNA Leaf tissue from each rice variety was extracted using a Qiagen DNeasy kit per manufacturer's protocols. DNA from each rice variety was added to Promega PCR Master Mix as per manufacturer's protocol for a 50 microliter reaction. Purification of PCR product was obtained by precipitating with a 9:1 ratio of 3M sodium acetate and 1:3 ratio of 100% ethanol. After 20 minute incubation at -20 Celsius, samples were centrifuged for 30 minutes. Ethanol was removed from the sample and the pellet containing purified PCR product was dried. PCR product was resuspended in double distilled water.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2017-12-31T17:02:37Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2017-03-28T12:09:31Z
attributes.tag: External Id
attributes.value: SAMEA103942078
attributes.tag: INSDC center name
attributes.value: OREGON STATE UNIVERS
attributes.tag: INSDC first public
attributes.value: 2017-12-31T17:02:37Z
attributes.tag: INSDC last update
attributes.value: 2017-03-28T12:09:31Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-5560:IR29-RBOHB-SSR
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: long-grained rice
attributes.tag: organism part
attributes.value: leaf
attributes.tag: sample name
attributes.value: E-MTAB-5560:IR29-RBOHB-SSR
attributes.tag: scientific_name
attributes.value: Oryza sativa Indica Group
attributes.tag: strain
attributes.value: IR29 PI 393986 OR06AR SD