title: Sample 3
organism: Yarrowia lipolytica
description: Protocols: Samples for RNA analysis were rapidly taken when the chemostats reached steady state, defined as stable CO2 and O2 outflow and optical density, which was achieved after circa 120 h. Samples were frozen in liquid nitrogen and stored at −80°C. Chemostat cultivation was performed with a dilution rate of 0.05 per hour at 30 °C in a 1.2 L bioreactor (DASGIP, Jülich, Germany) with a working volume of 750 ml at pH 3.5, controlled with 2 M KOH. Dissolved oxygen was kept above 30% with a stirrer rate of 600 rpm and an air flow rate of 1 v.v.m. Growth medium contained 25 g/L glucose, 0.5 g/L (NH4)2SO4, 5.96 g/L K2SO4, 3 g/L KH2PO4, 0.5 g/L MgSO4·7 H2O, vitamins and trace metal solutions and 125 μl antifoam 204 (Sigma-Aldrich, St Louis, MO, USA). Total RNA was extracted with Trizol (Invitrogen, Carlsbad, CA, USA) following the manufacturer's instructions with additional mechanical disruption of the cells using a FastPrep homogenizer (MP Biomedicals, Santa Ana, CA, USA) and 1 mm silica beads. Samples were depleted of rRNA using RiboZero
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-11-26T17:05:25Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-11-21T13:02:03Z
attributes.tag: External Id
attributes.value: SAMEA5097590
attributes.tag: INSDC center name
attributes.value: Department of Biology and Biological Engineering, Chalmers University of Technology
attributes.tag: INSDC first public
attributes.value: 2018-11-26T17:05:25Z
attributes.tag: INSDC last update
attributes.value: 2018-11-21T13:02:03Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7400:Sample 3
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: genotype
attributes.value: matA; leu2-270::leu2+; xpr2-332; axp-2; ku70::hph+; smooth-33
attributes.tag: growth condition
attributes.value: chemostat cultivation with nitrogen restriction conditions
attributes.tag: sample name
attributes.value: E-MTAB-7400:Sample 3
attributes.tag: scientific_name
attributes.value: Yarrowia lipolytica
attributes.tag: strain
attributes.value: FKP514