title: NHR-12 GFP L3 C.elegans ChIP Rep2
organism: Caenorhabditis elegans
attributes.tag: source_name
attributes.value: NHR-12 L3 ChIPRep2
attributes.tag: strain
attributes.value: OP318(official name : OP318 genotype : unc-119(ed3); wgIs318(nhr-12::TY1 EGFP FLAG; unc-119(+)) outcross : 3 mutagen : None tags : GFP::3xFlag description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Dresden using Tony Hyman's recombineering pipeline. The resulting plasmid was used for biolistic transformation of an unc-119(ed3) strain. The spatio-temporal expression pattern of NHR-12::EGFP fusion protein was examined through in vivo microscopy. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the NHR-12 transcription factor. made_by : Bob Waterston's lab from UW )
attributes.tag: developmental stage
attributes.value: L3
attributes.tag: genotype
attributes.value: unc-119(ed3); wgIs318(nhr-12::TY1 EGFP FLAG;unc-119(+))
attributes.tag: Sex
attributes.value: hermaphrodite