title: Sequence_Capture_of_25-36
organism: Gossypium hirsutum
description: Whole genome DNA samples were extracted using a modified CTAB protocol. Twelve samples were tagged with multiplex identifiers (MIDs), each with a different MID.  They were idependently hybridized to a custom Nimblegen Sequence Capture Array (12-plex).  After washing, the samples were all eluted into a single tube and subjected to ligation-mediated PCR (i.e. PCR using the 454 sequence adapters) to amplify the captured fragments to a high enough concentration for sequencing.  Subsequently, the libraries were prepared for 454 sequencing (size selection on a Capliper XL, emPCR, and bead preparation etc.).  The twelve captured samples tagged with multiplex identifiers (MIDs) were run on a single plate (2 large regions) of 454.  The samples were prepared using 'traditional' WGS libraries from Roche.  Below is a list of the samples and MIDs included in this run.  Sample Name MID MID Forward Sequence (in that order). TX_2002 1  ACGAGTGCGT; TX_2090 2  ACGCTCGACA; TX_2091 3  AGACGCACTC; TX_2092 4  AGCACTGTAG; TX_2093 5  ATCAGACACG; TX_2094 6  ATATCGCGAG; TX_2095 7  CGTGTCTCTA; TX_2096 8  CTCGCGTGTC; ARK_2402 9  TAGTATCAGC; ST825 10  TCTCTATGCG; TX_1988 11  TGATACGTCT; DP90 12  TACTGAGCTA;