title: Sample 23
organism: Mus musculus
description: Protocols: T helper type 2 cells were extracted from mouse spleens and activated with CD3/CD28. Each mouse replicate is a separate purification of T cells from a collection of mice. Mice were 8-12 weeks old and of mixed gender. Cells were spinoculated with retroviruses overexpressing a gene of interest on day 1. IL4 is 10ng/ml, and the cells were in treated with this for the remainder of the time spent in culture. For the overexpression for chipseq and rnaseq you can refer to Addgene plasmids #117263-117267. On day 4, BFP-positive (infected) cells were FACS sorted and measured by a variant of SMART-seq2. Further information can be found in the preprint here: https://www.biorxiv.org/content/early/2017/12/28/196022 or E-MTAB-6285. Lrrc40dec is a truncated version of Lrrc40. Cells were lysed with RLT+ and RNA purified using ampure XP beads A modified version of SMARTseq2
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-11-27T17:03:40Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-09-20T10:02:50Z
attributes.tag: External Id
attributes.value: SAMEA4925773
attributes.tag: INSDC center name
attributes.value: Sanger Institute / Karolinska Institutet
attributes.tag: INSDC first public
attributes.value: 2018-11-27T17:03:40Z
attributes.tag: INSDC last update
attributes.value: 2018-09-20T10:02:50Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7260:Sample 23
attributes.tag: age
attributes.value: 8 to 12
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: T-helper 2 cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: genotype
attributes.value: MSCV-Ccdc134-3xFLAG-IRES-BFP
attributes.tag: individual
attributes.value: mixed pool 2
attributes.tag: organism part
attributes.value: spleen
attributes.tag: phenotype
attributes.value: Ccdc134 overexpression
attributes.tag: sample name
attributes.value: E-MTAB-7260:Sample 23
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: mixed
attributes.tag: stimulus
attributes.value: CD3/CD28
attributes.tag: strain
attributes.value: CBLA