title: AM1
organism: Mus musculus
description: Protocols: Neurons and astrocytes were cultured as described in Bell et al., \Neuronal development is promoted by weakened intrinsic antioxidant defences due to epigenetic repression of Nrf2\, Nature Communications 6: 7066 (2015). Pure neuronal cultures or mixed astrocyte/neuronal cultures were treated ± tBHQ (10 µM) for 8h. At this point RNA was extracted and subject to RNA-seq analysis. RNA-seq libraries were prepared by Edinburgh Genomics using the Illumina TruSeq stranded mRNA-seq kit, according to the manufacturer's protocol (Illumina).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2017-12-31T17:01:43Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2017-05-16T18:32:10Z
attributes.tag: External Id
attributes.value: SAMEA104032772
attributes.tag: INSDC center name
attributes.value: unspecified
attributes.tag: INSDC first public
attributes.value: 2017-12-31T17:01:43Z
attributes.tag: INSDC last update
attributes.value: 2017-05-16T18:32:10Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-5688:AM1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: neuron
attributes.tag: common name
attributes.value: house mouse
attributes.tag: growth condition
attributes.value: Co-culture
attributes.tag: sample name
attributes.value: E-MTAB-5688:AM1
attributes.tag: scientific_name
attributes.value: Mus musculus