title: 16S rRNA gene fragments from surface layer of Marker 27 chimney in Edmond field.
organism: uncultured marine microorganism
attributes.tag: sample_name
attributes.value: DRS001480
attributes.tag: sample comment
attributes.value: Marker 27 chimney was an active black smoker chimney and was found at Edmond field (23Â°52.6660'S, 69Â°35.8075'E). The vent fluid temperature was 324.5Â°C. We sampled the chimney and divided into the surface and inside layer. We extracted DNA from each layer, and subjected to PCR amplification. But no fragment was amplified. Therefore, we analyzed only DNA fragments from surface l layer of the chimney. The amplified 100 bp of 16S rRNA gene fragments were used for 454 pyrosequencing analysis.