title: Sample 14
organism: Saccharina latissima
description: Protocols: Gametophytes from uniparental stock cultures (AWI seaweed collection) of S. latissima (AWI culture Number - males 3094, females 3096) from Helgoland, Germany were cultivated in glass flasks under red light (16hL:8hD) at 12°C in an environmentally controlled room. Seawater enriched with an adapted Provasoli medium without the addition of iron was replaced weekly. These conditions prevented the onset of gametogenesis (Lüning and Dring, 1975; Motomura and Sakai, 1981; Lewis et al., 2013), which was checked under the microscope regularly. To eliminate the confounding factor of red irradiance in our transcriptomic analysis, red light was replaced by white light with a photon fluence rate of 10 - 15 µmol photons m-2 s -1 (16hL:8hD) provided by fluorescent lamps OSRAM L 18W for one week prior to and during the experiment. These conditions retained our gametophytes' cultures at a vegetative stage. At the start of the experiment 3.10 +/- 0.10 g of fresh weight of female gametophyte clumps and 2.20 +/- 0.10 g of male gametophytes clumps were transferred separately to plastic petri dishes (n=4). Vegetative gametophytes were isolated from spores of fertile sporophyte collected at Helgoland, Germany according to the protocol of Bartsch I. (2018). Derivation of clonal stock cultures and hybridization of kelps. In Protocols for Macroalgae Research (pp. 61-78): CRC Press Taylor and Francis Group Total RNA extraction was conducted using the method described in Heinrich et al. 2012. Heinrich S, Frickenhaus S, Glöckner G, Valentin K (2012a) A comprehensive cDNA library of light- and temperature-stressed Saccharina latissima (Phaeophyceae). European Journal of Phycology, 47, 83-94.https://www.tandfonline.com/doi/abs/10.1080/09670262.2012.660639 cDNA libraries were prepared with an Illumina TruSeq RNA Library Prep Kit according to the manufacturer protocol.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-09-26T04:04:28Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-08-21T11:36:26Z
attributes.tag: External Id
attributes.value: SAMEA5882430
attributes.tag: INSDC center name
attributes.value: 1 Marine Botany, Faculty Biology/Chemistry, University of Bremen, Bremen, Germany 2 Station Biologique de Roscoff, plateforme ABiMS, CNRS: FR2424, Sorbonne Universite (UPMC), 29680 Roscoff, France 3Sorbonne Universite, CNRS, Integrative Biology of Marine Models (LBI2M), Station Biologique de Roscoff, 29680 Roscoff, France
attributes.tag: INSDC first public
attributes.value: 2019-09-26T04:04:28Z
attributes.tag: INSDC last update
attributes.value: 2019-08-21T11:36:26Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8267:Sample 14
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: developmental stage
attributes.value: gametophyte
attributes.tag: ecotype
attributes.value: Helgoland, Germany
attributes.tag: growth condition
attributes.value: long day length regimen
attributes.tag: organism part
attributes.value: whole organism
attributes.tag: sample name
attributes.value: E-MTAB-8267:Sample 14
attributes.tag: scientific_name
attributes.value: Saccharina latissima
attributes.tag: sex
attributes.value: male