title: L36_JQ1_1
organism: Homo sapiens
description: Protocols: Cells were treated for 24 hours before harvesting. Cells were treated for 24 hours before harvesting. RNA was isolated with QIAzol Lysis Reagent (Qiagen) added to the cells and centrifuged at full speed for 30 min. The aqueous phase was removed and mixed with equal volume of chloroform and centrifuged again for 20 minutes. The aqueous phase was then removed and the RNA was allowed to precipitate in isopropanol and incubated at -20°C overnight. Subsequently, RNA was washed twice with 70% ethanol and re-suspended in RNase free water. The integrity of RNA was validated by gel electrophoresis and 500ng were used to make the libraries in triplicates for each condition. Libraries were made using the TruSeq RNA Library Prep Kit V2 (Illumina) according to the manufacturer's instructions.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-10-01T04:02:45Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-09-17T16:21:47Z
attributes.tag: External Id
attributes.value: SAMEA4920512
attributes.tag: INSDC center name
attributes.value: Department of General, Visceral and Pediatric Surgery, University Medical Center Gottingen, 37075 Gottingen, Germany
attributes.tag: INSDC first public
attributes.value: 2019-10-01T04:02:45Z
attributes.tag: INSDC last update
attributes.value: 2018-09-17T16:21:47Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7216:L36_JQ1_1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: L3.6pl
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: pancreatic adenosquamous carcinoma
attributes.tag: metastatic site
attributes.value: celiac axis lymph node
attributes.tag: organism part
attributes.value: pancreas; liver
attributes.tag: sample name
attributes.value: E-MTAB-7216:L36_JQ1_1
attributes.tag: scientific_name
attributes.value: Homo sapiens