title: Old mouse, transgenic 5xFAD, treated with HSP, sample ID 126
organism: Mus musculus
description: Adult males of NMRI mice (11 months old) and transgenic 5xFAD mice were used in experiments. Transgenic (Tg) and non-transgenic (NTg) 5XFAD mice treated with the same doze of Hsp70 (2 μg/mouse in 4μl intranasal injections) daily for one month belong to two age groups. “Young mice”-4months (y_*) and “aging mice”-11 months (o_*). The animals were maintained in their home cages in a climate-controlled room (21-23 °C) with a 12:12 hour light-dark cycle and had free access to water and food. 5xFAD mice (TG6799). These mice co-express the Swedish (K670N/M671L), Florida (I716V) and London (V717I) mutations in human APP(695), and M146L and L286V mutations in PS1, with expression of both transgenes driven by Thy1 promoter. Transgenic mice were acquired from Charles River laboratories and maintained on a mixed SJL/C57Bl6. Mice were genotyped by PCR analysis of DNA extracted from ears biopsies. The transgenic cassette was detected using primers 5’-AGGACTGACCACTCGACCAG-3’ and 5’-CGGGGGTCTAGTTCTGCAT-3’, yielding a 377bp product.
attributes.tag: strain
attributes.value: 5XFAD
attributes.tag: age
attributes.value: 11 months
attributes.tag: sex
attributes.value: male
attributes.tag: tissue
attributes.value: hippocampus
attributes.tag: biomaterial_provider
attributes.value: Institute of Cell Biophysics, Russian Academy of Sciences, Pushchino
attributes.tag: replicate
attributes.value: biological replicate 2
attributes.tag: sample_type
attributes.value: Hippocampus tissue sample
attributes.tag: store_cond
attributes.value: -80C
attributes.tag: treatment
attributes.value: Hsp70 (2 μg/mouse in 4μl intranasal injections) daily for one month belong to two age groups
attributes.tag: BioSampleModel
attributes.value: Model organism or animal