title: Sequence_capture_of_37-48
organism: Gossypium hirsutum
description: Despite the 3635 Taxon ID, this sequence capture sample contains some reads from diploids related to G. histutum.  See the Sample Names below. Whole genome DNA samples were extracted using a modified CTAB protocol. Twelve samples were tagged with multiplex identifiers (MIDs), each with a different MID.  They were independently hybridized to a custom Nimblegen Sequence Capture Array (12-plex).  After washing, the samples were all eluted into a single tube and subjected to ligation-mediated PCR (i.e. PCR using the 454 sequence adapters) to amplify the captured fragments to a high enough concentration for sequencing.  Subsequently, the libraries were prepared for 454 sequencing (size selection on a Capliper XL, emPCR, and bead preparation etc.).    The twelve captured samples tagged with multiplex identifiers (MIDs) were run on a single plate (2 large regions) of 454.  The samples were prepared using 'traditional' WGS libraries from Roche.  Below is a list of the samples and MIDs included in this run.  Sample Name MID MID Forward Sequence (in that order).   Sample Name MID MID Forward Sequence  Coker_315 1  ACGAGTGCGT;  Cascot_L7 2  ACGCTCGACA;  Lkt_511 3  AGACGCACTC;  PM_145 4  AGCACTGTAG;  FM_958 5  ATCAGACACG;  BR_110 6  ATATCGCGAG;  Maxxa 7  CGTGTCTCTA;  D5 8  CTCGCGTGTC;  AD3 9  TAGTATCAGC;  GK 10  TCTCTATGCG;  TAM 11  TGATACGTCT;  A2 12  TACTGAGCTA;