title: 16S rRNA gene fragments from surface layer of White Head chimney in Edmond field.
organism: uncultured marine microorganism
attributes.tag: sample_name
attributes.value: DRS001479
attributes.tag: sample comment
attributes.value: White Head chimney was an active clear smoker chimney and was found at Edmond field (23Â°52.6772'S, 69Â°35.7847'E). The top of the chimney was covered with white sulfur particle. The vent fluid temperature was 264Â°C. We sampled the chimney and divided into the surface and inside layer. We extracted DNA from each layer, and subjected to PCR amplification. But no fragment was amplified. Therefore, we analyzed only DNA fragments from surface l layer of the chimney. The amplified 200 bp of 16S rRNA gene fragments were used for 454 pyrosequencing analysis.