title: LPS24_IL7_704
organism: Homo sapiens
description: Protocols: Peripheral blood was obtained from genotyped individuals recruited via the Oxford biobank (www.oxfordbiobank.org.uk) with full ethical approval (REC: 06/Q1605/55) and written informed consent. Genotype was blinded at the time of study and only revealed at the end of recruitment. Peripheral blood and synovial fluid samples from were recruited with informed consent from patients with inflammatory arthritis attending the Oxford University Hospitals NHS Foundation Trust (Ethics reference number 06/Q1606/139). The AllPrep DNA/RNA/miRNA kit (Qiagen) was used for RNA extraction. Cells were spun down and re-suspended in 350μl of RLTplus buffer and transferred to 2ml tubes. Samples were then stored at -80°C for batched RNA extraction. Homogenization of the sample was carried out using the QIAshredder (Qiagen). DNase I was used during the extraction protocol to minimise DNA contamination. RNA was eluted into 35μl of RNasefree water. The RNA amount was quantified by qubit and the RNA samples stored at - 80°C for storage until ready for sequencing. RNA underwent quality control testing using a bioanalyser (RNA 6000 Nano kit, Agilent) followed by cDNA library preparation.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-08-17T04:02:47Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-08-07T12:07:44Z
attributes.tag: External Id
attributes.value: SAMEA5857662
attributes.tag: INSDC center name
attributes.value: Department of Oncology, University of Oxford
attributes.tag: INSDC first public
attributes.value: 2019-08-17T04:02:47Z
attributes.tag: INSDC last update
attributes.value: 2019-08-07T12:07:44Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8225:LPS24_IL7_704
attributes.tag: age
attributes.value: 50
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: mononuclear cell
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: individual
attributes.value: 704
attributes.tag: organism part
attributes.value: blood
attributes.tag: sample name
attributes.value: E-MTAB-8225:LPS24_IL7_704
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: female
attributes.tag: stimulus
attributes.value: LPS24+IL7