title: 0_1H6_IP_replicate2
organism: Homo sapiens
description: Protocols: HL-60/S4 cells were cultured in RPMI-1640 medium with 10% heat-inactivated fetal calf serum and 1% penicillin/streptomycin. Cells were differentiated into granulocytes by addition of all-trans ratinoic acid and into macrophage by phorbol ester. Cells were fixed, lysed and incubated with primary antibody, either PL2-6 or 1H6, before they were fixed again and sonicated in Covaris micro-chambers. The harvested supernatant was incubated with Protein A/G agarose to bind the epichromatin-antibody complex, and the remainder (input) represents the total nuclear chromatin. The epichromatin (IP) was eluted from the complex. DNA was purified with QIAquick PCR Purification Kit (QIAGEN). ChIP-seq libraries were prepared with NEB Next Chip Seq DNA Sample Prep Kit according to the manufacturers protocol.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2015-12-31T17:01:12Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T17:13:26Z
attributes.tag: External Id
attributes.value: SAMEA2395808
attributes.tag: INSDC center name
attributes.value: DKFZ
attributes.tag: INSDC first public
attributes.value: 2015-12-31T17:01:12Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T17:13:26Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-2360:0_1H6_IP_replicate2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: HL-60/S4
attributes.tag: cell type
attributes.value: undifferentiated myeloid leukemic cell
attributes.tag: common name
attributes.value: human
attributes.tag: sample name
attributes.value: E-MTAB-2360:0_1H6_IP_replicate2
attributes.tag: scientific_name
attributes.value: Homo sapiens