title: 16S rRNA gene fragments from surface layer of Kali chimney in Kairei field.
organism: uncultured marine microorganism
attributes.tag: sample_name
attributes.value: DRS001474
attributes.tag: sample comment
attributes.value: Kali site was located at east end of Kairei field (25Â°19.2233'S, 70Â°02.3720'E) and we found active black smoker chimneys in this site. The vent fluid temperature was 362Â°C. We sampled the chimney and divided into the surface and inside layer. We extracted DNA from each layer and subjected to PCR amplification. The amplified 100 bp of 16S rRNA gene fragments were used for 454 pyrosequencing analysis.