title: Macleaya cordata phase1 root
organism: Macleaya cordata
description: Total RNA was extracted by modified CTAB method 41from 10 different tissues of the Macleaya cordata  (Willd.) R. Br., including Phase I(roots, leaves) and Phase II(roots, leaves and shells) which were snap-frozen and stored at -80*C until processing. RNA integrity was confirmed using the Agilent 2100 Bioanalyzer with a minimum integrity number value of 8. Equal amounts of total RNA from each tissue were prepared for cDNA preparation.