title: OT-15-Ang-20-X-S
organism: soil metagenome
description: Ouémé Supérieur soil transect 2015, Angaradebou sample #20, preserved with Xpedition, Short amplicon
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-05-18T04:06:29Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-03-20T11:12:15Z
attributes.tag: External Id
attributes.value: SAMEA6631807
attributes.tag: INSDC center name
attributes.value: UPPSALA UNIVERISTY
attributes.tag: INSDC first public
attributes.value: 2020-05-18T04:06:29Z
attributes.tag: INSDC last update
attributes.value: 2020-03-20T11:12:15Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: OT-15-Ang-20-X-S
attributes.tag: amount or size of sample collected
attributes.value: 0.125 L
attributes.tag: collection date
attributes.value: 2015-05
attributes.tag: common name
attributes.value: soil metagenome
attributes.tag: environment (biome)
attributes.value: tropical savannah ENVO:01000188
attributes.tag: environment (feature)
attributes.value: ectomycorrhizal woodland
attributes.tag: environment (material)
attributes.value: tropical soil ENVO:00005778
attributes.tag: experimental factor
attributes.value: 20 m from transect origin
attributes.tag: geographic location (country and/or sea)
attributes.value: Benin
attributes.tag: geographic location (depth)
attributes.value: 0-0.05
attributes.tag: geographic location (elevation)
attributes.value: 320
attributes.tag: geographic location (latitude)
attributes.value: N 9.75456
attributes.tag: geographic location (longitude)
attributes.value: W 2.14064
attributes.tag: geographic location (region and locality)
attributes.value: Borgou department, N'Dali Commune, Forêt Classée de l'Ouémé Supérieur near Angaradebou village
attributes.tag: investigation type
attributes.value: metagenome
attributes.tag: nucleic acid extraction
attributes.value: Zymo Research Xpedition Soil Microbe DNA MiniPrep D6202
attributes.tag: pcr conditions
attributes.value: initial denaturation:95degC_10min; denaturation:95degC_60s; annealing:56degC_45s; extension:72degC_50s; cycles:35; final extension:72degC_3min
attributes.tag: pcr primers
attributes.value: gITS7mod-67: CCATCTCATCCCTGCGTGTCTCCGACTCAGTTCCTACCAGTCGATGTGARTCATCGARTCTTTG; ITS4: TCCTCCGCTTATTGATATGC, ITS4A: TCCTCGCCTTATTGATATGC
attributes.tag: project name
attributes.value: ena-STUDY-UPPSALA UNIVERISTY-19-03-2020-10:28:50:771-6
attributes.tag: sample name
attributes.value: OT-15-Ang-20-X-S
attributes.tag: sample volume or weight for DNA extraction
attributes.value: 0.05
attributes.tag: sample weight for DNA extraction
attributes.value: 0.25
attributes.tag: scientific_name
attributes.value: soil metagenome
attributes.tag: sequencing method
attributes.value: ION_TORRENT, PACBIO_SMRT, ILLUMINA
attributes.tag: soil environmental package
attributes.value: soil
attributes.tag: storage conditions (fresh/frozen/other)
attributes.value: field lysis by bead beating in Xpedition lysis solution (Zymo Research)
attributes.tag: target gene
attributes.value: ITS2