title: Functional_S3
organism: Homo sapiens
description: Protocols: Cells were sorted using fluorescence-activated cell sorting, pelleted, and frozen. Nucleic acids were extracted from frozen cell pellets using a Qiagen all prep DNA/RNA extraction kit One microgram of purified RNA was reverse-transcribed and PCR amplified using gene specific primers with truncated Illumina adapter primers. The initial PCR reaction was separated on a DNA agarose gel and the PCR band was extracted and gel purified using a DNA extraction kit (Thermo). The purified product was amplified using the final segments of the Illumina adapter primers and purified using a DNA clean-up kit (Thermo). Samples were then quantified and submitted for Illumina MiSeq analysis.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-10-01T04:02:55Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-09-20T16:36:15Z
attributes.tag: External Id
attributes.value: SAMEA5790048
attributes.tag: INSDC center name
attributes.value: UNIVERSITY OF MINNESOTA
attributes.tag: INSDC first public
attributes.value: 2019-10-01T04:02:55Z
attributes.tag: INSDC last update
attributes.value: 2019-09-20T16:36:15Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8357:Functional_S3
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: HEK293T
attributes.tag: common name
attributes.value: human
attributes.tag: fraction
attributes.value: functional HIV-Vif variants
attributes.tag: infect
attributes.value: Human immunodeficiency virus
attributes.tag: sample name
attributes.value: E-MTAB-8357:Functional_S3
attributes.tag: scientific_name
attributes.value: Homo sapiens