title: Sample 9
organism: Triticum aestivum
description: Protocols: endosperm isolation. rolled out from grain Glasshouse growth. 16h photoperiod 25/19 oC total RNA isolation. As in Pellny et al. (2012) Library Preparation_RNA-Seq PolyA. The mRNA fraction will be selected from the total RNA provided before conversion to cDNA. Second strand cDNA synthesis will incorporate dUTP. The cDNA will be end-repaired, A-tailed and adapter-ligated. Prior to amplification, samples will undergo uridine digestion. The prepared libraries will be size selected, multiplexed and QC'ed
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-10-01T04:02:54Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-08-09T13:39:29Z
attributes.tag: External Id
attributes.value: SAMEA5860540
attributes.tag: INSDC center name
attributes.value: ROTHAMSTED RESEARCH
attributes.tag: INSDC first public
attributes.value: 2019-10-01T04:02:54Z
attributes.tag: INSDC last update
attributes.value: 2019-08-09T13:39:29Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8237:Sample 9
attributes.tag: age
attributes.value: 17
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: bread wheat
attributes.tag: cultivar
attributes.value: Cadenza
attributes.tag: developmental stage
attributes.value: whole plant fruit ripening stage
attributes.tag: genotype
attributes.value: TaIRX9b triple homozygous knockout
attributes.tag: growth condition
attributes.value: long day length regimen
attributes.tag: organism part
attributes.value: endosperm
attributes.tag: sample name
attributes.value: E-MTAB-8237:Sample 9
attributes.tag: scientific_name
attributes.value: Triticum aestivum