title: Global analysis of DNA methylation in early-stage liver fibrosis
organism: Mus musculus
description: The genomic DNA from each of 4 mouse livers treated with 2 ml/kg CCl4 for 2 weeks were pooled.  The genomic DNA from 3 mouse livers treated with olive oil for 2 weeks was pooled and used as control.  The genomic DNA purified from the CCl4-treated mouse liver specimens was randomly fragmented into 50–350 bp lengths as described in the SOLiD 5500xl fragment library protocol.  The fragmented DNA was then subjected to the MethylMiner methylated DNA enrichment kit according to the manufacturer’s protocol.  These methylated fragments were eluted with 1000 mM NaCl and used to construct standard fragment libraries using a combination of adaptor ligation and nick translation (SOLiD Fragment Library Construction Kit, Invitrogen).  Each DNA library was selected on the basis of size (inserts were approximately 500 bp) by AgencourtAMPure XP (Beckman) before PCR amplification, bead attachment, and emulsion PCR.