title: 33S
organism: Homo sapiens
description: Protocols: All samples were placed in RNAlater RNA Stabilization Reagent (QIAGEN, Germany) and stored at -80ºC before RNA extraction. Total RNA was extracted with TRIzol (Ambion, USA) reagent according to the manufacturer's instructions. A barcoded cDNA library was first generated with SuperScript® VILO™ cDNA Synthesis kit from 10 ng of total RNA. Then cDNA amplified using Ion AmpliSeq™ Transcriptome Human Gene Expression Kit (ThermoFisher) to accurately maintain expression levels of all targeted genes. The average size of each amplicon is ~150 bp. Amplified cDNA Libraries evaluated for quality and quantified using Agilent Bioanalyzer High sensitivity chip. Libraries were then diluted to 100pM and pooled equally, with eight individual samples per pool. Pooled libraries amplified and enriched by using IonChef Instrument (ThermoFisher) according to manufacturer instructions.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-09-30T17:04:23Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-07-10T10:47:01Z
attributes.tag: External Id
attributes.value: SAMEA5766282
attributes.tag: INSDC center name
attributes.value: Laboratory of Preclinical & Translational Research, IRCCS-CROB Referral Cancer Center of Basilicata
attributes.tag: INSDC first public
attributes.value: 2019-09-30T17:04:23Z
attributes.tag: INSDC last update
attributes.value: 2019-07-10T10:47:01Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8135:33S
attributes.tag: age
attributes.value: 79
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: gastric carcinoma
attributes.tag: individual
attributes.value: 33
attributes.tag: organism part
attributes.value: stomach
attributes.tag: sample name
attributes.value: E-MTAB-8135:33S
attributes.tag: sampling site
attributes.value: normal tissue adjacent to neoplasm
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: male