title: SKU-DT-L3
organism: Oryza sativa
description: Protocols: Oryza sativa cv. Nipponbare seeds were used in all experiments. Seeds germinated at 28 C in dark for 2 days were transferred to plant growth chamber to grow for 14 days under controlled conditions (12 h light 30 C/12h dark 24 C cycles) to harvest the seedlings. For stress treatments, 14-day-old seedlings were incubated in solutions containing 20% PEG-6000, 200 mM NaCl, for 4 h at 30 C, respectively. For cold treatment seedlings at the same developmental stage were treated at 4 C for 24 h in dark. Total genomic DNA was extracted from leaf tissues using DNase (New England Biolabs), and total RNA was isolated using the TRIzol reagent (Invitrogen). The OligoTex mRNA midi kit (Qiagen) was used to purify poly(A) mRNA from the total RNA samples. Next, mRNAs were fragmented by the RNA fragmentation kit (Ambion). The first cDNA strand was synthesized using random hexamer primers and the second strand cDNA was synthesized where dUTP was used instead of dTTP. Then the dUTP was digested. Small RNAs, ranging from 18-35 bp were enriched by polyacrylamide gel electrophoresis, and ligated to 5' and 3' adapters. The ligation product was reverse-transcript into cDNA, which was then amplified by 15 PCR cycles.
attributes.tag: ENA first public
attributes.value: 2011-12-28
attributes.tag: ENA last update
attributes.value: 2018-03-08
attributes.tag: External Id
attributes.value: SAMEA1031593
attributes.tag: INSDC center alias
attributes.value: NCGR
attributes.tag: INSDC center name
attributes.value: National Center for Gene Research, Chinese Academy of Sciences
attributes.tag: INSDC first public
attributes.value: 2011-12-28T17:00:35Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T15:27:10Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-721:SKU-DT-L3
attributes.tag: age
attributes.value: 14 days
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: rice
attributes.tag: growth condition
attributes.value: drought stress
attributes.tag: organism part
attributes.value: seedling
attributes.tag: sample name
attributes.value: E-MTAB-721:SKU-DT-L3