title: Arabidopsis thaliana 3 week seedlings
organism: Arabidopsis thaliana
description: Briefly, A. thaliana seeds were surface sterilized with 75% ethanol, plated on half strength Murashige and Skoog (MS) agar medium at 4 C in the dark for 3 days until uniform germination, and then were germinated on at 22 C in constant light for 10 days. The seedlings with about similar size were transferred to potting mix for growth in 22 C growth chambers with 16-h light/8-h dark cycles. The plant materials of whole seedlings were collected at day 21 after initiation of germination. Genomic DNA was extracted using a DNeasy Plant Mini kit (Qiagen) following manufacturers' instructions.
attributes.tag: ecotype
attributes.value: Col-0
attributes.tag: dev_stage
attributes.value: 3 week seedlings
attributes.tag: geo_loc_name
attributes.value: USA
attributes.tag: tissue
attributes.value: seedlings
attributes.tag: BioSampleModel
attributes.value: Plant