title: Complete genome sequence of a new Ebola virus strain, isolated from the 2017 Likati Ebola outbreak in Democratic Republic of the Congo.
organism: Zaire ebolavirus
description: As genomic sequencing can greatly facilitate public health efforts to identify and characterize the infectious agent as well as to contain and monitor an epidemic, our research team was deployed to undertake real-time sequencing using the Oxford Nanopore Technologies MinION™ sequencer in support of the local diagnostic teams during the May 2018 Ebola virus outbreak in the Equateur province of Democratic Republic of the Congo (DRC). As a pilot study, we investigated a confirmed Ebola-positive blood sample from the May 2017 Likati outbreak at the Institut National de Recherche Biomédicale (INRB), Kinshasa. The sample, collected on 7 May 2017, originated from a 22-year-old male (onset of disease 30 April 2017, deceased 8 May 2017). Here, we present the complete genome sequence of this Ebola virus isolate, obtained using MinION™ sequencer. We were able to generate results in less than 24 hours, with the sequencing workflow started from the viral RNA extraction to raw MinION data generation.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-04-10T04:03:03Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-04-09T18:12:37Z
attributes.tag: External Id
attributes.value: SAMEA5561116
attributes.tag: INSDC center name
attributes.value: KU LEUVEN - UNIVERSITY OF LEUVEN
attributes.tag: INSDC first public
attributes.value: 2019-04-10T04:03:03Z
attributes.tag: INSDC last update
attributes.value: 2019-04-09T18:12:37Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: 1
attributes.tag: collecting institution
attributes.value: Institut National de Recherche Biomédicale
attributes.tag: collection date
attributes.value: 2017-05-07
attributes.tag: collector name
attributes.value: INRB
attributes.tag: geographic location (country and/or sea)
attributes.value: Democratic Republic of the Congo
attributes.tag: geographic location (latitude)
attributes.value: 3.35 N
attributes.tag: geographic location (longitude)
attributes.value: 23.88 E
attributes.tag: geographic location (region and locality)
attributes.value: Likati health zone in the province of Bas Uele
attributes.tag: host age
attributes.value: 22
attributes.tag: host common name
attributes.value: Human
attributes.tag: host disease outcome
attributes.value: dead
attributes.tag: host health state
attributes.value: not provided
attributes.tag: host scientific name
attributes.value: Homo sapiens
attributes.tag: host sex
attributes.value: male
attributes.tag: host subject id
attributes.value: EVD sample 12
attributes.tag: isolate
attributes.value: Zaire ebolavirus
attributes.tag: sample capture status
attributes.value: active surveillance in response to outbreak
attributes.tag: sample name
attributes.value: 1
attributes.tag: scientific_name
attributes.value: Zaire ebolavirus
attributes.tag: virus identifier
attributes.value: Zaire ebolavirus