title: Col-0_H2A.W
organism: Arabidopsis thaliana
description: Protocols: Tissues were fixed in 1% (v/v) formaldehyde and flash frozen in liquid nitrogen. Plants were grown in vitro in a growth cabinet at 23°C, 50% humidity, using long day conditions (16h light, 8h dark). Tissues were homogenized in liquid nitrogen. After nuclei isolation and lysis, chromatin was sonicated in a Covaris S220 following manufacturer's instructions and shearing efficiency was verified on gel. Immunoprecipitation with specific antibodies was followed by reverse-crosslink and phenol-choloroform DNA purification. Libraries were constructed with the NuGEN Ovation Ultra Low System V2 kit, following the manufacturer's instructions.
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism
attributes.value: Arabidopsis thaliana
attributes.tag: ecotype
attributes.value: Col-0
attributes.tag: age
attributes.value: 10
attributes.tag: developmental stage
attributes.value: mid rosette growth stage
attributes.tag: organism part
attributes.value: rosette
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-05-19
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-05-11