title: B6-10-TSPAN8-
organism: Mus musculus
description: Protocols: TEC were isolated via enzymatic digestion of thymic lobes using Liberase (Roche) and DNaseI (Roche). Cells were counted and stained with anti-CD45 microbeads (Miltenyi Biotec) for 15 minutes at room temperature, before negative selection using the AutoMACS (Miltenyi Biotec) system in order to enrich for TEC. Samples were then stained for cell surface markers for 20 minutes at 4°C and were FACS sorted into 1.5ml DNase/RNase free Eppendorf tubes pre-coated with BSA and containing 150µl of plain RPMI-1640. TSPAN8+/- (CD45-EpCAM+ -> LY51-CD80+ -> TSPAN8+/-); GP2+/- (CD45-EpCAM+ -> LY51-UEA1+ -> GP2+/-); Unselected (GP2+/- (CD45-EpCAM+ -> LY51-UEA1+). 10x genomics Chromium Single Cell 3' V1 kit or V2 kit. 10x genomics Chromium Single Cell 3' V1 kit or V2 kit.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-10-01T04:02:54Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-07-30T14:58:05Z
attributes.tag: External Id
attributes.value: SAMEA5818858
attributes.tag: INSDC center name
attributes.value: MRC Human Genetics Unit, IGMM, The University of Edinburgh
attributes.tag: INSDC first public
attributes.value: 2019-10-01T04:02:54Z
attributes.tag: INSDC last update
attributes.value: 2019-07-30T14:58:05Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8105:B6-10-TSPAN8-
attributes.tag: age
attributes.value: 4 to 6
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: medullary thymic epithelial cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: thymus lobe
attributes.tag: phenotype
attributes.value: CD45negative; EpCAMpositive; LY51negative; CD80positive; TSPAN8negative;
attributes.tag: sample name
attributes.value: E-MTAB-8105:B6-10-TSPAN8-
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: C57BL/6