title: Land_use_LUSIGNAN_soil_bacteria_fungi_diversity_study
organism: soil metagenome
attributes.tag: ENA first public
attributes.value: 2015-12-31
attributes.tag: ENA last update
attributes.value: 2016-10-21
attributes.tag: External Id
attributes.value: SAMEA2729955
attributes.tag: INSDC center alias
attributes.value: INRA-CMSE
attributes.tag: INSDC center name
attributes.value: UMR Agroecology, INRA - Burgundy University, Dijon, France
attributes.tag: INSDC first public
attributes.value: 2015-12-31T17:01:12Z
attributes.tag: INSDC last update
attributes.value: 2016-10-21T09:33:12Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: LUS_18S_C2-R-T9
attributes.tag: amount or size of sample collected
attributes.value: 200
attributes.tag: collection date
attributes.value: 2012-05-24
attributes.tag: depth
attributes.value: 0.1
attributes.tag: environment (biome)
attributes.value: temperate grasslands
attributes.tag: environment (feature)
attributes.value: field soil
attributes.tag: environment (material)
attributes.value: Cambisol
attributes.tag: geographic location (countryand/orsea,region)
attributes.value: France
attributes.tag: geographic location (elevation)
attributes.value: 85
attributes.tag: geographic location (latitude)
attributes.value: 46.413789
attributes.tag: geographic location (longitude)
attributes.value: 0.122089
attributes.tag: history/crop rotation
attributes.value: Wheat/Barley/Maize
attributes.tag: history/previous land use
attributes.value: Cropland
attributes.tag: investigation type
attributes.value: mimarks-survey
attributes.tag: multiplex identifiers
attributes.value: CGTGCTGTGT
attributes.tag: pcr conditions
attributes.value: 5ng of DNA were used for a 25 µl PCR conducted under the following conditions: initial denaturation : 94degC_3min; 35 cycles of: denaturation : 94degC_1min; annealing : 52degC_1min ; elongation: 72degC_1min, final elongation : 72degC_5min. A second PCR of 9 cycles was then conducted under similar PCR conditions with purified PCR products and ten base pair multiplex identifiers added to the primers at 5' position to specifically identify each sample and avoid PCR biases.
attributes.tag: pcr primers
attributes.value: FR1 (5'-ANCCATTCAATCGGTANT-3') FF390 (5'-CGATAACGAACGAGACCT-3')
attributes.tag: ph
attributes.value: 6.58
attributes.tag: project name
attributes.value: LAND-USE_LUSIGNAN_2014
attributes.tag: sample name
attributes.value: LUS_18S_C2-R-T9
attributes.tag: sample weight for DNA extraction
attributes.value: 1
attributes.tag: sequencing method
attributes.value: 454-pyrosequencing
attributes.tag: soil environmental package
attributes.value: soil
attributes.tag: soil type
attributes.value: Cambisol
attributes.tag: storage conditions (fresh/frozen/other)
attributes.value: lyophilized + frozen
attributes.tag: target gene
attributes.value: 18S rRNA gene
attributes.tag: texture
attributes.value: 18/69/13
attributes.tag: water content of soil
attributes.value: 0.21