title: WT1
organism: Mus musculus
description: Protocols: Lungs were removed from mice and stored in RNAlater solution (Thermo fisher scientific)until being processed. B-16V WT or BAG6KO cells were cultured in CD293 particle-free medium for 48h under hypoxic conditions before EVs were isolated from supernatants by Ultracentrifugation (precentrifugation 5min 300xg, 10 min 2000xg and 20 min 3500xg; ultracentrifugation at 100k x g)Mice were intravenously injected with 20 µg WT BAG6KO EVs in 50µl PBS, or 50µl PBS control, for 4 weeks on a weekly basis. Tissue homogenization of lungs stored in RNAlater solution (Thermo fisher scientific) for total RNA extraction using gentle MACS (Mitenyi biotec) followed by RNA isolation using RNeasy Mini Kit (Qiagen) including on-column DNA digest using RNase-Free DNase Set (Qiagen), RNA elution in H20 Illumina truseq mRNA kit v2
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-11-30T11:01:58Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-08-08T14:15:42Z
attributes.tag: External Id
attributes.value: SAMEA4821601
attributes.tag: INSDC center name
attributes.value: Center for Immune and Tumor Biology
attributes.tag: INSDC first public
attributes.value: 2018-11-30T11:01:58Z
attributes.tag: INSDC last update
attributes.value: 2018-08-08T14:15:42Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7119:WT1
attributes.tag: age
attributes.value: 8 to 12
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: lung
attributes.tag: sample name
attributes.value: E-MTAB-7119:WT1
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: C57BL/6J