title: ES_mock_antiL3MBTL2
organism: Mus musculus
description: Protocols: Mouse J1 ES Cells were cultivated feeder-cell free on gelatin-coated plates in DMEM + GlutaMax (Gibco), supplemented with 15% fetal bovine serum (Biochrom), 1% non-essential amino acids (Gibco), 1% Penicillin/Streptomycin (Sigma), 50mM beta-Mecraptoethanol and 1000U/ml ESGRO leukmia inhibitory factor (Merck Millipore). ChIP was performed using the One Day ChIP Kit (Diagenode) according to manufacturer's instructions. Three to four individual ChIPs were pooled and purifed on QIAquick columns (Qiagen). Five nanograms of precipitated DNA were used for indexd deep sequency library preperation using the Microplex library preparation kit v2 (Diagenode). Libraries were purified on AMPure magnetic beads (Beckman Coulter).]
attributes.tag: ENA first public
attributes.value: 2018-01-01
attributes.tag: ENA last update
attributes.value: 2017-08-24
attributes.tag: External Id
attributes.value: SAMEA104225672
attributes.tag: INSDC center alias
attributes.value: Institute of Molecular Biology and Tumor Resarch (IMT), Philipps-University of Marburg, Marburg, Germany
attributes.tag: INSDC center name
attributes.value: Institute of Molecular Biology and Tumor Resarch (IMT), Philipps-University of Marburg, Marburg, Germany
attributes.tag: INSDC first public
attributes.value: 2018-01-01T17:01:42Z
attributes.tag: INSDC last update
attributes.value: 2017-08-24T17:01:08Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6007:ES_mock_antiL3MBTL2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: J1
attributes.tag: cell type
attributes.value: embryonic stem cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: sample name
attributes.value: E-MTAB-6007:ES_mock_antiL3MBTL2