title: Mus musculus fetal liver
organism: Mus musculus
description: Mouse erythroleukemia (MEL) cells and primary erythroid progenitors (obtained from fetal liver of E13.5 mouse embryos) were cultured as previously described(Soler et al., 2010). Primary mouse erythrocytes were differentiated at day 3 or 4 of ex vivo culturing by transferring them to StemPro-34 Serum-Free medium supplemented with 2 mM L-Glutamine, penicillin/streptomycin, 10U/ml EPO and 1 mg/ml Transferrin for 16 hours. Mouse dermal fibroblasts (MDF) were maintained under low oxygen (3%) conditions in DMEM/Ham’s F-10 medium (50%/50%, Lonza). All animal experiments were carried out according to institutional and national guidelines.
attributes.tag: dev_stage
attributes.value: E13.5
attributes.tag: age
attributes.value: 12.5
attributes.tag: tissue
attributes.value: liver