title: E-MTAB-7115:Human_009h
organism: Homo sapiens
attributes.tag: Alias
attributes.value: E-MTAB-7115:Human_009h
attributes.tag: Broker name
attributes.value: ArrayExpress
attributes.tag: Description
attributes.value: Protocols: BLaER1 cells are as described here: https://doi.org/10.1016/j.celrep.2013.03.003 and are derived as a subclone of Seraphina cells CVCL_M646. C10 cells are as described https://doi.org/10.1016/j.stem.2009.10.004 and are derived as a subclone of the transformed fetal liver HAFTL-1 cell line. Fluorescent staining of cell surface markers was done with antibodies against Mac1 (APC) and CD19 (APC-Cy7) (BD Pharmigen). Samples were analyzed on the LSRII flow cytometer (BD Biosciences) and data were analyzed with FlowJo software (Tree Star). Human BLaER1 cells and mouse C10 cells were grown in RPMI (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum, 2 mM L-glutamine, and 100 U/ml penicillin G sodium. Transdifferentiation was induced in BlaER1 and C10 cells by addition of 100nM of β-estradiol (Calbiochem) and grown with 10nM of IL-3 and CSF-1 (Peprotech). Cells were lysed with Qiazol following the manufacturer's instructions (Qiagen). Chloroform was added to each sample and RNA contained in the aqueous fraction was isolated and purified by using RNeasy mini kit columns also following the manufacturer's instructions (Qiagen). Stranded poly A+ libraries were prepared with 1 ug of total RNA using TruSeq mRNA Sample Prep Kit (Illumina) according to manufacturer's protocol.
attributes.tag: ENA checklist
attributes.value: ERC000011
attributes.tag: INSDC center alias
attributes.value: Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Bioinformatics and Genomics.
attributes.tag: INSDC center name
attributes.value: Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Bioinformatics and Genomics.
attributes.tag: INSDC first public
attributes.value: 2018-11-30T11:01:58Z
attributes.tag: INSDC last update
attributes.value: 2018-08-03T10:51:39Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: SRA accession
attributes.value: ERS2634169
attributes.tag: Sample Name
attributes.value: ERS2634169
attributes.tag: Title
attributes.value: Human_009h
attributes.tag: cell_line
attributes.value: BLaER1
attributes.tag: cell_type
attributes.value: B cell
attributes.tag: disease
attributes.value: Epstein-Barr virus-related Burkitts lymphoma
attributes.tag: organism
attributes.value: Homo sapiens
attributes.tag: organism part
attributes.value: blood