organism: Homo sapiens
description: hindlimb cell suspension
attributes.tag: Biomaterial type
attributes.value: cell_suspension
attributes.tag: dissociation_protocol - method
attributes.value: enzymatic dissociation
attributes.tag: dissociation_protocol - protocol_core - protocol_description
attributes.value: Samples were chopped into a mash using a scalpel and incubated in 5ml of 2.5mg/ml liberase at 37 degrees for 30-40 min till fully digestion. Cells were spun down at 4C 750 for 5min and resuspended in 3ml of 1X RBC lysis buffer (eBioscience 00-4333, or diluted from eBioscience 00-4300) followed by 4-5 min incubation at RT. 10ml of 1X PBS was then added. The cells were then spun down at 4C at 750 for 5min, resuspended in 5ml of 2% FBS in 1X PBS, filtered through a 40um strainer, spun down again iat 4C 750g for 5min, resuspended in 50-200ul of 2% FBS in PBS, and spun down again.
attributes.tag: dissociation_protocol - protocol_core - protocol_id
attributes.value: dissociation
attributes.tag: dissociation_protocol - protocol_core - protocol_name
attributes.value: Single cell isolation from human fetal thigh
attributes.tag: estimated_cell_count
attributes.value: 4498
attributes.tag: project
attributes.value: Human Cell Atlas
attributes.tag: sample name
attributes.value: cell_suspension_7