title: Naive rep1
organism: Mus musculus
description: Protocols: CD4+, CD62L+ cells were purified with CD4+CD62L+ T Cell Isolation Kit II (Miltenyi Biotec) and further purification was performed by FACS using anti-CD62L and anti-CD4 Fab fragments. Poly-(A)+ RNA was purified from ~500,000 cells using the Oligotex kit (Qiagen). The manufacturers protocol was modified slightly to include additional final elution steps resulting in a larger volume. RNA was purified, reverse transcribed and prepared for sequencing described in http://msb.embopress.org/content/7/1/497
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2015-04-05T17:04:12Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T17:48:51Z
attributes.tag: External Id
attributes.value: SAMEA2536558
attributes.tag: INSDC center name
attributes.value: EBI
attributes.tag: INSDC first public
attributes.value: 2015-04-05T17:04:12Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T17:48:51Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-2582:Naive rep1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: naive thymus-derived CD4-positive, alpha-beta T cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: sample name
attributes.value: E-MTAB-2582:Naive rep1
attributes.tag: scientific_name
attributes.value: Mus musculus