title: 4453
organism: Gallus gallus
description: Protocols: Spleen tissues were collected from each chicken at day 2 or day 6 post infection. At 21 days posthatch (dph), the chickens were divided randomly into two groups separated by different rooms. One group was treated with the LaSota lentogenic strain of NDV (200 ul of 108 EID50 for each chicken) through nasal and ocular inoculation routes (50 ul into each eye and nostril), while the control group was treated with 200ul phosphate-buffered saline (PBS) for each chicken through the same routes. RNA samples was extracted from spleen tissue using Ambion RNAqueous Total RNA Isolation Kit (Thermo Fisher Scientific) following the manufacturer's protocol. The transcriptome library of each sample was constructed with 0.5 µg total RNA using the Illumina TruSeq RNA sample preparation kit (Illumina Inc., San Diego, CA) following the low sample protocol in the TruSeq RNA sample preparation v2 guide (Part #15026495, March 2014).The proper length of cDNA in each library was guaranteed by running the DNA 1000 assay on the Agilent 2100 Bioanalyzer. Libraries from 2 and 6 dpi were independently constructed and sequenced at different times.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-09-30T04:05:20Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-09-20T10:50:56Z
attributes.tag: External Id
attributes.value: SAMEA5789128
attributes.tag: INSDC center name
attributes.value: IOWA STATE UNIVERSITY
attributes.tag: INSDC first public
attributes.value: 2019-09-30T04:05:20Z
attributes.tag: INSDC last update
attributes.value: 2019-09-20T10:50:56Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8350:4453
attributes.tag: age
attributes.value: 23
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: chicken
attributes.tag: organism part
attributes.value: spleen
attributes.tag: sample name
attributes.value: E-MTAB-8350:4453
attributes.tag: scientific_name
attributes.value: Gallus gallus
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: Hy-Line Brown
attributes.tag: time
attributes.value: 2