title: AGPolle_Sample_M
organism: Fagus sylvatica
description: Protocols: A 100 cm^2 area of bark was peeled of a living tree. Developing xylem was scraped from the surface underneath and directly frozen in liquid nitrogen. Mint-2 Kit for cDNA preparation (Evrogen) & cDNA normalization TRIMMER kit (Evrogen) Frozen tissue was ground under liquid nitrogen in a ball mill (Retsch, Haan, Germany). Tissue powder (500 mg) was used for RNA extraction after Chang et al. (1993) with minor modifications: the extraction buffer contained 2% b-mercaptoethanol and no spermindine. RNA was purified (RNeasy Mini Kit, Qiagen, Venlo, Netherlands). Total RNA yield and purity were determined spectrophotometrically (NanoDrop, Thermo Fisher Scientific, Waltham, MA, USA). RNA integrity was assessed on an Agilent 2100 Bioanalyzer (Agilent, Santa Clara, CA, USA). TruSeq RNA Library Preparation Kit (Illumina)
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2016-01-01T17:01:22Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-09T01:31:31Z
attributes.tag: External Id
attributes.value: SAMEA3446419
attributes.tag: INSDC center name
attributes.value: Dept. of Tree Physiology and Forest Botany University of Gottingen
attributes.tag: INSDC first public
attributes.value: 2016-01-01T17:01:22Z
attributes.tag: INSDC last update
attributes.value: 2018-03-09T01:31:31Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-3615:AGPolle_Sample_M
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: European beech
attributes.tag: harvest timepoint
attributes.value: div.
attributes.tag: individual
attributes.value: blend
attributes.tag: organism part
attributes.value: developing xylem
attributes.tag: sample name
attributes.value: E-MTAB-3615:AGPolle_Sample_M
attributes.tag: sampling site
attributes.value: Calvoerde & Goerde
attributes.tag: scientific_name
attributes.value: Fagus sylvatica