title: MCF-7_jc33
organism: Homo sapiens
description: Protocols: MCF-7 cells were grown in DMEM containing 10% heat-inactivated FBS, 2 mM L glutamine, 50 U/ml penicillin and 50 _g/ml streptomycin and ZR75-1 cells were grown in RPMI containing 10% heat-inactivated FBS, 2 mM L glutamine, 50 U/ml penicillin and 50 _g/ml streptomycin. MCF10A cells were maintained in Mammary Epithelium Cell Growth Medium bullet kit (Clonetics, Lonza, MD, USA), containing mammary epithelium basal medium supplemented with bovine pituitary extract, human epidermal growth factor, hydrocortisone and GA-1000 (Gentamicin Sulfate and Amphotericin-B). To hormone deprive the cells, cells were grown in steroid-depleted medium for three days and then treated with vehicle (ethanol), 100 nM estrogen or 1 uM tamoxifen for 45 minutes or three hours. The antibodies used were anti-ER (sc-543) from Santa Cruz Biotechnologies and anti-CTCF (07-729) from Millipore. At least four 15 cm dishes of cells were used per chromatin immunoprecipitation (ChIP) and samples were processed according to standard ChIP procedures. The CTCF ChIP samples were performed as described in Carroll, 2005, Cell and the ER ChIP samples were performed as described in Schmidt, 2009, Methods. The immunoprecipitated DNA was subsequently amplified as previously described for Illumina sequencing Schmidt, 2009, Methods.
attributes.tag: ENA first public
attributes.value: 2011-12-16
attributes.tag: ENA last update
attributes.value: 2018-03-08
attributes.tag: External Id
attributes.value: SAMEA1032055
attributes.tag: INSDC center alias
attributes.value: CRUK-CRI
attributes.tag: INSDC center name
attributes.value: CRUK-CRI
attributes.tag: INSDC first public
attributes.value: 2011-12-16T16:59:35Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T15:27:48Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-740:MCF-7_jc33
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: MCF-7
attributes.tag: cell type
attributes.value: epithelial
attributes.tag: common name
attributes.value: human
attributes.tag: organism part
attributes.value: breast
attributes.tag: sample name
attributes.value: E-MTAB-740:MCF-7_jc33
attributes.tag: sex
attributes.value: female