title: Pig 26
organism: Sus scrofa
description: Protocols: Endothelial cells were obtained from adult pigs immediately after euthanasia at a local slaughterhouse. The left ventricle was dissected revelaing the chamber lumen. The ventricle was rinsed with ice-cold PBS. Endothelial cells were freshly harvested by gentle scraping of regions (1-2 cm2) located at the apex, the posterior mid-ventricle (midv) and the outflow tract (OT), just beneath the aortic valve. Endothelial cells were obtained from adult pigs immediately after euthanasia at a local slaughterhouse. The left ventricle was dissected revelaing the chamber lumen. The ventricle was rinsed with ice-cold PBS. Endothelial cells were freshly harvested by gentle scraping of regions (1-2 cm2) located at the apex, the posterior mid-ventricle (midV) and the outflow tract (OT), just beneath the aortic valve. Total RNA was isolated by mirVana miRNA Isolation Kit (Life Technologies). The quality of RNA was evaluated by NanoDrop 1000 Spectrophotometer and TapeStation 2200 (Agilent Technologies). TruSeq RNA Sample prep library construction
attributes.tag: ENA first public
attributes.value: 2015-12-31
attributes.tag: ENA last update
attributes.value: 2018-03-08
attributes.tag: External Id
attributes.value: SAMEA3466668
attributes.tag: INSDC center alias
attributes.value: University of Pennsylvania
attributes.tag: INSDC center name
attributes.value: University of Pennsylvania
attributes.tag: INSDC first public
attributes.value: 2015-12-31T17:01:09Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T15:25:24Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-3669:Pig 26
attributes.tag: age
attributes.value: ~ 6 months
attributes.tag: breed
attributes.value: Landrace x Yorkshire
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: pig
attributes.tag: organism part
attributes.value: heart
attributes.tag: region
attributes.value: apex
attributes.tag: sample name
attributes.value: E-MTAB-3669:Pig 26
attributes.tag: sex
attributes.value: male