title: 16S rRNA gene fragments from in situ colonization system deployed into Kali hydrothermal vent.
organism: uncultured marine microorganism
attributes.tag: sample_name
attributes.value: DRS001475
attributes.tag: sample comment
attributes.value: Kali site was located at east end of Kairei field (25Â°19.2233'S, 70Â°02.3720'E) and we found active black smoker chimneys in this site. The vent fluid temperature was 362Â°C. We have deployed in situ colonization system into the vent to capture microbes habited in the rocks beneath the hydrothermal area. We extracted DNA from porous careers in ISCS and subjected to PCR amplification. The amplified 100 bp of 16S rRNA gene fragments were used for 454 pyrosequencing analysis.