title: NS20_2_d27_S15
organism: Homo sapiens
description: Protocols: GnRH enriched cells are GnRH positive cells selected in FACS analysis using our HEL11.4 GNRH1-reporter cell line generated via CRISPR/Cas9. Cells were sorted and pooled using a FACS machine GNRH1 neurons were differentiated based a protocol previously published (Lund C et al., 2016, Stem cell reports) During the differentiation, after initial treatment with Dorsomporphin and SB431542, FGF8 treatment was continued with DAPT treatment in the terminal step Using RNAqueous micro Total RNA Isolation kit (Thermo Scietific) RNA was isolated. RNA was analyzed for concentration, integrity and quality using Qubit Fluorometer and TapeStation 4200 (Agilent). NEBNext Ultra Directional polyA capture method as paired-end sequencing for read length 75 bp
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-09-30T04:05:16Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-05-07T10:32:33Z
attributes.tag: External Id
attributes.value: SAMEA5595252
attributes.tag: INSDC center name
attributes.value: Department of Physiology, Faculty of Medicine, University of Helsinki
attributes.tag: INSDC first public
attributes.value: 2019-09-30T04:05:16Z
attributes.tag: INSDC last update
attributes.value: 2019-05-07T10:32:33Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7938:NS20_2_d27_S15
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: HEL11.4
attributes.tag: cell type
attributes.value: neuron
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: CRISPR-Cas9 induced GNRH1 reporter
attributes.tag: organism part
attributes.value: skin
attributes.tag: progenitor cell type
attributes.value: induced pluripotent stem cell
attributes.tag: sample name
attributes.value: E-MTAB-7938:NS20_2_d27_S15
attributes.tag: scientific_name
attributes.value: Homo sapiens