title: Paramecium tetraurelia PacBio RSII sequencing T50 stage (Library 1+2, ipdSummary analysis)
organism: Paramecium tetraurelia
description: Total genomic DNA was extracted from wild type Paramecium tetraurelia cells (strain 51 mating type 7) at a late developmental stage (T50) of the self-fertilization process of autogamy. 2 SMRTbell libraries were prepared (size selection step using a 15kb (Library 1) or 13kb (Library 2) cutoff) then were sequenced on 16 SMRTcells on the RSII instrument. After alignment, sequencing data from all SMRTcells was merged. Then, 6mA were detected using the ipdSummary.py script.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-12-15T17:12:14Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-09-17T18:29:02Z
attributes.tag: External Id
attributes.value: SAMEA7320726
attributes.tag: INSDC center name
attributes.value: INSTITUT JACQUES MONOD
attributes.tag: INSDC first public
attributes.value: 2020-12-15T17:12:14Z
attributes.tag: INSDC last update
attributes.value: 2020-09-17T18:29:02Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: PTET_WT_T50_PACBIORSII_SEQ_Library1and2_ipdSummary
attributes.tag: dev stage
attributes.value: T50
attributes.tag: sample name
attributes.value: PTET_WT_T50_PACBIORSII_SEQ_Library1and2_ipdSummary
attributes.tag: scientific_name
attributes.value: Paramecium tetraurelia
attributes.tag: strain
attributes.value: 51