title: E-MTAB-7115:Mouse_030m
organism: Mus musculus
description: Protocols: BLaER1 cells are as described here: https://doi.org/10.1016/j.celrep.2013.03.003 and are derived as a subclone of Seraphina cells CVCL_M646. C10 cells are as described https://doi.org/10.1016/j.stem.2009.10.004 and are derived as a subclone of the transformed fetal liver HAFTL-1 cell line. Fluorescent staining of cell surface markers was done with antibodies against Mac1 (APC) and CD19 (APC-Cy7) (BD Pharmigen). Samples were analyzed on the LSRII flow cytometer (BD Biosciences) and data were analyzed with FlowJo software (Tree Star). Human BLaER1 cells and mouse C10 cells were grown in RPMI (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum, 2 mM L-glutamine, and 100 U/ml penicillin G sodium. Transdifferentiation was induced in BlaER1 and C10 cells by addition of 100nM of β-estradiol (Calbiochem) and grown with 10nM of IL-3 and CSF-1 (Peprotech). Cells were lysed with Qiazol following the manufacturer's instructions (Qiagen). Chloroform was added to each sample and RNA contained in the aqueous fraction was isolated and purified by using RNeasy mini kit columns also following the manufacturer's instructions (Qiagen). Stranded poly A+ libraries were prepared with 1 ug of total RNA using TruSeq mRNA Sample Prep Kit (Illumina) according to manufacturer's protocol.
attributes.tag: ENA checklist
attributes.value: ERC000011
attributes.tag: INSDC center alias
attributes.value: Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Bioinformatics and Genomics.
attributes.tag: INSDC center name
attributes.value: Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Bioinformatics and Genomics.
attributes.tag: INSDC first public
attributes.value: 2018-11-30T11:01:58Z
attributes.tag: INSDC last update
attributes.value: 2018-08-03T10:51:40Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: SRA accession
attributes.value: ERS2634185
attributes.tag: Sample Name
attributes.value: ERS2634185
attributes.tag: alias
attributes.value: E-MTAB-7115:Mouse_030m
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: C10
attributes.tag: cell type
attributes.value: precursor B cell
attributes.tag: disease
attributes.value: normal
attributes.tag: organism
attributes.value: Mus musculus
attributes.tag: organism part
attributes.value: liver
attributes.tag: title
attributes.value: Mouse_030m