title: MR36
organism: Homo sapiens
description: Protocols: The hESCs H9 (WA-09)-NURR1-GFP reporter line was maintained using E8-essential medium (Fisher Scientific) without feeder on VTA-N (Fisher Scientific) and passaged every 4-5 days by EDTA. Midbrain dopamine (mDA) differentiation from hESC was done with an optimized protocol from previously published by our group. mock (solvent) or 50uM 6OHDA treatment for 16h ChIP was performed and DNA was purified with a magnetic bead-based purification method according to the manufacturer's protocol (MAGnify Chromatin Immunoprecipitation System-Kit, LifeTechnologies). sheared DNA was used to generate ChIP-sequencing libraries by employing Ovation Ultralow System V2 Kit (NuGEN).
attributes.tag: ENA first public
attributes.value: 2018-07-22
attributes.tag: ENA last update
attributes.value: 2017-08-15
attributes.tag: External Id
attributes.value: SAMEA104215622
attributes.tag: INSDC center alias
attributes.value: The Rockefeller University Laboratory of Molecular and Cellular Neuroscience
attributes.tag: INSDC center name
attributes.value: The Rockefeller University Laboratory of Molecular and Cellular Neuroscience
attributes.tag: INSDC first public
attributes.value: 2018-07-22T17:01:55Z
attributes.tag: INSDC last update
attributes.value: 2017-08-15T15:26:59Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-5965:MR36
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: H9
attributes.tag: cell type
attributes.value: dopaminergic neuron
attributes.tag: common name
attributes.value: human
attributes.tag: genotype
attributes.value: NURR1::GFP
attributes.tag: progenitor cell type
attributes.value: embryonic stem cell
attributes.tag: sample name
attributes.value: E-MTAB-5965:MR36