title: m30
organism: Homo sapiens
description: Protocols: Cells were grown in regular DMEM or in DMEM lacking Met/Cys for different time points (6-30-120 hours for regular DMEM or 6-15-30-72-120 hours for medium w/o Met/Cys) . Cells were trypsinized and collected by centrifugation, then immediately frozen at -80°C. Total RNA was prepared using Trizol (Sigma), to preserve transcripts of both small and long sizes, and DNase treated to avoid contamination of genomic DNA. Ovation RNA-Seq System V2
attributes.tag: ENA first public
attributes.value: 2018-07-21
attributes.tag: ENA last update
attributes.value: 2018-02-01
attributes.tag: External Id
attributes.value: SAMEA104558735
attributes.tag: INSDC center alias
attributes.value: Center for Translational Genomics and Bioinformatics, IRCCS San Raffaele Scientific Institute
attributes.tag: INSDC center name
attributes.value: Center for Translational Genomics and Bioinformatics, IRCCS San Raffaele Scientific Institute
attributes.tag: INSDC first public
attributes.value: 2018-07-21T17:02:53Z
attributes.tag: INSDC last update
attributes.value: 2018-02-01T14:46:39Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6452:m30
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: HeLa
attributes.tag: cell type
attributes.value: epithelial cell
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: cervical adenocarcinoma
attributes.tag: genotype
attributes.value: pcDNA3.1-OA1
attributes.tag: growth condition
attributes.value: DMEM without methionine or cysteine
attributes.tag: organism part
attributes.value: uterine cervix
attributes.tag: sample name
attributes.value: E-MTAB-6452:m30