title: MTLAB_fleQ1
organism: Pseudomonas syringae pv. tomato str. DC3000
description: Protocols: The strains were grown on LB plates for 48 hours and resuspended in 3 ml of 10 mM MgCl2. Ten ml of MMR minimal medium (Robertsen et al., 1982) were inoculated and the OD adjusted to an OD660 of 0.05. The strains were grown in MMR minimal medium at 20o C to an OD660=1.5. At OD660=1.5 in MMR liquid minimal medium, the cells were harvested, washed with sarkosyl 0.1% and cell pellets were immediately frozen in liquid nitrogen and conserved at -80oC until RNA isolation. rRNA removal with Ribo zero magnetic kit was performed by Macrogen. The cells were processed for RNA isolation using TRI Reagent®LS (Molecular Research Center, Cincinnati, OH, USA) according to the manufacturer instructions, except that the TRI Reagent was preheated at 70oC and the lysis step was carried out at 65oC. The RNA obtained was treated with RNase-free DNase I (Quiagen) followed by purification with RNeasy mini kit (Quiagen).RNA concentration was determined spectrophotometrically and its integrity was assessed by agarose gel electrophoresis. cDNA libraries were constructed by Macrogen.
attributes.tag: ENA first public
attributes.value: 2015-12-31
attributes.tag: ENA last update
attributes.value: 2015-07-29
attributes.tag: External Id
attributes.value: SAMEA3493445
attributes.tag: INSDC center alias
attributes.value: Estacion Experimental del Zaidin (CSIC)
attributes.tag: INSDC center name
attributes.value: Estacion Experimental del Zaidin (CSIC)
attributes.tag: INSDC first public
attributes.value: 2015-12-31T17:01:16Z
attributes.tag: INSDC last update
attributes.value: 2015-07-29T16:59:09Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-3779:MTLAB_fleQ1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: genotype
attributes.value: fleQ mutant
attributes.tag: growth condition
attributes.value: liquid medium
attributes.tag: sample name
attributes.value: E-MTAB-3779:MTLAB_fleQ1