title: WT rep2
organism: Mus musculus
description: Protocols: Gene expression profiling analyses by high throughput sequencing were performed on mRNA samples isolated from the sense Pja2 in HT-22 cells (Pja2/+) and the wildtype cells. HT-22 were routinely maintained in the conventional mouseEagle's medium RNA was harvested using Trizol reagent.TruSeq® RNA LT Sample Prep Kit v2 was used with total RNA for the construction of sequencing libraries. RNA was harvested using Trizol reagent.TruSeq® RNA LT Sample Prep Kit v2 was used with total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard BGISEQ500 protocols
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-12-15T04:05:20Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-01-28T17:12:05Z
attributes.tag: External Id
attributes.value: SAMEA5278088
attributes.tag: INSDC center name
attributes.value: School of Life Sciences, Anhui University
attributes.tag: INSDC first public
attributes.value: 2020-12-15T04:05:20Z
attributes.tag: INSDC last update
attributes.value: 2019-01-28T17:12:05Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6464:WT rep2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: HT-22
attributes.tag: cell type
attributes.value: neuron
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: mouse postnatal
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: growth condition
attributes.value: Eagles medium
attributes.tag: sample name
attributes.value: E-MTAB-6464:WT rep2
attributes.tag: scientific_name
attributes.value: Mus musculus