title: AM.IP
organism: Homo sapiens
description: Protocols: PDAC tissue from surgical samples was fragmented, mixed with 100 μL of Matrigel and implanted with a trocar (10 gauge; Innovative Research of America, Sarasota, FL) in the subcutaneous right upper flank of an anesthetized and disinfected male NMRI-nude mouse. Samples obtained from EUS-FNA were mixed with 100 μL of Matrigel (BD Biosciences, Franklin Lakes, NJ) and injected in the upper right flank of a male nude mice (Swiss Nude Mouse Crl: NU(lco)-Foxn1nu; Charles River Laboratories, Wilmington, MA) for the first implantation. When xenografts reached 1 cm3, these were removed and passed to NMRI-nude mice in the same manner as the surgical samples. All animal experiments were conducted in accordance with institutional guidelines and were approved by the “Plateforme de Stabulation et d'Expérimentation Animale” (PSEA, Scientific Park of Luminy, Marseille). RNA was extracted using RNeasy mini kit with optional on column DNA digestion (Qiagen). RNA libraries were prepared (Illumina TruSeq mRNA v2) and run on the Illumina High Seq-2000 for 101 bp paired end reads
attributes.tag: ENA first public
attributes.value: 2017-12-30
attributes.tag: ENA last update
attributes.value: 2017-06-28
attributes.tag: External Id
attributes.value: SAMEA104143852
attributes.tag: INSDC center alias
attributes.value: Mayo Clinic
attributes.tag: INSDC center name
attributes.value: Mayo Clinic
attributes.tag: INSDC first public
attributes.value: 2017-12-30T17:01:52Z
attributes.tag: INSDC last update
attributes.value: 2017-06-28T10:14:18Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-5639:AM.IP
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: pancreatic adenocarcinoma
attributes.tag: growth condition
attributes.value: xenograft in nude mouse
attributes.tag: individual
attributes.value: AM.IP
attributes.tag: organism part
attributes.value: neoplasm
attributes.tag: phenotype
attributes.value: basal
attributes.tag: sample name
attributes.value: E-MTAB-5639:AM.IP