title: sRNAs from SINV-infected HEK293 cells expressing Dicer-2, 16 hpi
organism: Homo sapiens
attributes.tag: source_name
attributes.value: HEK293 cell line
attributes.tag: cell line
attributes.value: HEK293
attributes.tag: construct
attributes.value: pFlag-HA-Dicer2-Puro
attributes.tag: growth protocol
attributes.value: HEK293 cells were maintained in Dulbecco's modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS) in a humidified atmosphere of 5% CO2 at 37°C.
attributes.tag: viral infection condition
attributes.value: 16 hours post-infection, SINV MOI 0.01
attributes.tag: extract protocol
attributes.value: Total RNA was extracted from infected cells using TRI-Reagent (Ambion, AM9738)