title: biological replicate 2
organism: Rattus norvegicus
attributes.tag: strain
attributes.value: not applicable
attributes.tag: isolate
attributes.value: The S1_2 rats were trained to self-administer heroin under a fixed ratio paradigm for 4 h daily in operant chambers which equipped with two nose pokes (ENV-114 M, Med Associates, St. Albans, VT). Each response in the active hole was immediately reinforced with an infusion of heroin (0.5 mg/kg) via the pump over 4 s, at the same time was accompanied by the noise of the infusion pump and illumination of the stimulus light located above the active nose poke for 20 s. The number of responses during this 20 s period was recorded, but there was no delivery of heroin. Daily training sessions were conducted over 14 consecutive days. The criterion of stable heroin self-administration was defined as less than 10% of the variability in the number of active nose poking responses that rats touched on the last 3 days .
attributes.tag: breed
attributes.value: Rat
attributes.tag: cultivar
attributes.value: Sprague Dawley
attributes.tag: ecotype
attributes.value: Sprague Dawley
attributes.tag: age
attributes.value: 6-8 weeks
attributes.tag: dev_stage
attributes.value: 250 to 300 g
attributes.tag: sex
attributes.value: male
attributes.tag: tissue
attributes.value: Nucleus accumbens
attributes.tag: BioSampleModel
attributes.value: Model organism or animal