title: run38_s5
organism: Schizosaccharomyces pombe
description: Protocols: All cDNA samples used for Illumina sequencing were prepared by first treating ~1 mg of total RNA for 30 min with amplification grade RNase-free DNase (Invitrogen), according to the manufacturer's protocols. PolyA enriched RNA was then prepared using an oligo(dT) selection kit (Oligotex Direct mRNA MiniKit, Qiagen). The resulting poly(A)-enriched RNA was then converted to double-stranded cDNA using a cDNA synthesis kit (Superscript choice system for cDNA synthesis, Invitrogen) , primed by an oligo(dT) primer, according to the manufacturer's protocols. Random-primed RNA from rapidly proliferating cells was treated with an enzyme that specifically degrades non-capped RNA molecules (mRNA-only™ Eukaryotic mRNA isolation kit, Epicentre Biotechnologies) according to the manufacturer's protocols. The resulting cDNA was then converted to double-stranded cDNA as above, except that the reaction was primed with random hexamers. RNA samples from the pooled meiotic timepoints were subjected to IVT amplification after undergoing a poly(A) enrichment step as described above. RNA from the oxidative stress condition and prp2 mutant was treated in the same way as the rapidly proliferating samples with oligo(dT)-primed cDNA synthesis after mRNA enrichment.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2011-12-15T17:00:21Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T15:33:40Z
attributes.tag: External Id
attributes.value: SAMEA1035313
attributes.tag: INSDC center name
attributes.value: SC
attributes.tag: INSDC first public
attributes.value: 2011-12-15T17:00:21Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T15:33:40Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: StrainOrLine
attributes.value: JB371
attributes.tag: Submitter Id
attributes.value: E-MTAB-5:run38_s5
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: fission yeast
attributes.tag: genotype
attributes.value: pat1-114/pat1-114 ade6-M210/ade6-M216 h+/h+
attributes.tag: sample name
attributes.value: E-MTAB-5:run38_s5
attributes.tag: scientific_name
attributes.value: Schizosaccharomyces pombe
attributes.tag: sex
attributes.value: mating_type_h_plus/plus