title: L1
organism: soil metagenome
description: Long term abandoned grassland (26-29 years)
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2015-12-31T17:01:14Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-09T12:30:12Z
attributes.tag: External Id
attributes.value: SAMEA3219687
attributes.tag: INSDC center name
attributes.value: INRA-CMSE
attributes.tag: INSDC first public
attributes.value: 2015-12-31T17:01:14Z
attributes.tag: INSDC last update
attributes.value: 2018-03-09T12:30:12Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Level of intensification of land-use
attributes.value: low
attributes.tag: Submitter Id
attributes.value: LTO_18S_L1
attributes.tag: amount or size of sample collected
attributes.value: 500
attributes.tag: collection date
attributes.value: 2011-10
attributes.tag: current land use
attributes.value: Pasture
attributes.tag: current vegetation
attributes.value: grassland
attributes.tag: depth
attributes.value: 0.1
attributes.tag: environment (biome)
attributes.value: temperate grasslands
attributes.tag: environment (feature)
attributes.value: field soil
attributes.tag: environment (material)
attributes.value: Podzols
attributes.tag: geographic location (country and/or sea)
attributes.value: Netherlands
attributes.tag: geographic location (elevation)
attributes.value: 36
attributes.tag: geographic location (latitude)
attributes.value: 52.0291719
attributes.tag: geographic location (longitude)
attributes.value: 5.801076111111111
attributes.tag: investigation type
attributes.value: mimarks-survey
attributes.tag: multiplex identifiers
attributes.value: CAGACGTCTG
attributes.tag: pcr conditions
attributes.value: 5ng of DNA were used for a 25µl PCR conducted under the following conditions: initial denaturation: 94degC_3.0min; 35 cycles of denaturation: 94degC_1.0min; annealing: 52degC_1.0min;elongation: 72degC_1.0min;final elongation:72degC_5.0min. A second PCR of 9 cycles was then conducted under similar PCR conditions with purified PCR products and ten base pair multiplex identifiers added to the primers at 5' position to specifically identify each sample and avoid PCR biases.
attributes.tag: pcr primers
attributes.value: FR1 :5-ANCCATTCAATCGGTANT-3 ; FF390 :5-CGATAACGAACGAGACCT-3
attributes.tag: ph
attributes.value: 5.6
attributes.tag: pooling of DNA extracts (ifdone)
attributes.value: yes
attributes.tag: project name
attributes.value: LTO_div_functioning
attributes.tag: sample name
attributes.value: LTO_18S_L1
attributes.tag: sample weight for DNA extraction
attributes.value: 2
attributes.tag: scientific_name
attributes.value: soil metagenome
attributes.tag: sequencing method
attributes.value: pyrosequencing
attributes.tag: soil environmental package
attributes.value: soil
attributes.tag: storage conditions (fresh/frozen/other)
attributes.value: frozen
attributes.tag: target gene
attributes.value: 18S rRNA gene
attributes.tag: texture
attributes.value: 84/10/6
attributes.tag: total n
attributes.value: 1.69
attributes.tag: total organic carbon
attributes.value: 32.2