title: A549_dex_4C-seq_Rep1
organism: Homo sapiens
description: Protocols: Cells were cultured to confluency and treated for 90 min with 1μM dexamethasone. 4C experiments were performed as previously described (van de Werken et al., Methods Enzymol, 2012. 513: p. 89-112.), using 5x10^6 cells. 4-bp cutters were used as primary (CviQI, Thermo Fisher Scientific) and secondary (DpnII, Thermo Fisher Scientific) restriction enzymes. For each cell line 4C libraries were generated in two biological replicates. Based on 4C-libraries we amplified a total of 1.2 μg of DNA by inverse-PCR using viewpoint-specific primers (fwd: CTACACGACGCTCTTCCGATCTAATGTTCAGGTGTGGGAGTAC, rev: CAGACGTGTGCTCTTCCGATCTGCCCTCTGCCTCTTGTTAGG), which include adaptors for subsequent high throughput sequencing (Illumina HiSeq2500).
attributes.tag: ENA first public
attributes.value: 2017-12-31
attributes.tag: ENA last update
attributes.value: 2016-12-19
attributes.tag: External Id
attributes.value: SAMEA30153418
attributes.tag: INSDC center alias
attributes.value: Abt Vingron Max Planck Institute for Molecular Genetics
attributes.tag: INSDC center name
attributes.value: Abt Vingron Max Planck Institute for Molecular Genetics
attributes.tag: INSDC first public
attributes.value: 2017-12-31T17:02:35Z
attributes.tag: INSDC last update
attributes.value: 2016-12-19T17:53:50Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-5350:A549_dex_4C-seq_Rep1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: A549
attributes.tag: common name
attributes.value: human
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: sample name
attributes.value: E-MTAB-5350:A549_dex_4C-seq_Rep1
attributes.tag: stimulus
attributes.value: dexamethasone