title: 12PB7801
organism: Homo sapiens
description: Protocols: Peripheral blood was collected from patients with primary myelofibrosis (PMF) or post ET- or PV-myelofibrosis (MF). Patients were part of of the MPNSG-0109 study (Pomalidomide in myeloproliferative neoplasm-associated myelofibrosis. Schlenk RF, Stegelmann F et al., Leukemia 2017). HREC approval 45/07 Universität Ulm Ethikkommission. Most patients were untreated at sample collection, apart from two patients treated with Pomalidomide and one patient treated with Hydroxyurea. RNA was extracted RNA libraries were prepared using the NEBnext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs), assessed for size and quantified using the 2100 Bioanalyzer (Agilent Technologies) and Kapa Library Quantitation Kit (Illumina) respectively.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-12-01T17:02:15Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-10-01T09:55:40Z
attributes.tag: External Id
attributes.value: SAMEA4947841
attributes.tag: INSDC center name
attributes.value: Gordon and Jessie Gilmour Leukaemia Research Laboratory, QIMR Berghofer Medical Research Institute
attributes.tag: INSDC first public
attributes.value: 2018-12-01T17:02:15Z
attributes.tag: INSDC last update
attributes.value: 2018-10-01T09:55:40Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7255:12PB7801
attributes.tag: age
attributes.value: 78
attributes.tag: asxl1 gene
attributes.value: wild type
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: calr gene
attributes.value: wild type
attributes.tag: clinical history
attributes.value: primary myelofibrosis
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: primary myelofibrosis
attributes.tag: dnmt3a gene
attributes.value: c.G517T.pG173c
attributes.tag: genotype
attributes.value: JAK2VF DNMT3A
attributes.tag: jak2vf gene
attributes.value: mutation
attributes.tag: mpl gene
attributes.value: wild type
attributes.tag: organism part
attributes.value: blood
attributes.tag: sample name
attributes.value: E-MTAB-7255:12PB7801
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: female
attributes.tag: srsf2 gene
attributes.value: wild type
attributes.tag: tet2 gene
attributes.value: wild type