title: BrDi.W4.mix.1
organism: Brachypodium distachyon
description: Protocols: Leaf material was collected and flash frozen i) in the afternoon (8 hours after lights on) before cold treatment and the next day 8 hours after cold treatment and ii) in the morning (at lights on) before cold treatment, 4 weeks after cold treatment and 9 weeks after cold treatment. Seeds were germinated and initially grown in a greenhouse at a neutral day length (12 hours of light), 17°C and a minimum artificial light intensity of 150 µmol/m2s. Depending on the species, one to eight week old plants were randomized and distributed to two cold chambers with short day (8 hours of light), 6°C and a light intensity of 50 µmol/m2s. Cold (6°C) with short day (8 hours of light), and a light intensity of 50 µmol/m2s. Flash frozen leaves were individually homogenized using a TissueLyser (Qiagen Retsch) and total RNA was isolated (from each leaf) using RNeasy Plant Mini Kit (Qiagen) following the manufacturer's instructions. The purity and integrity of total RNA extracts was determined using a NanoDrop 8000 UV-Vis Spectrophotometer (Thermo Scientific) and 2100 Bioanalyzer (Agilent), respectively. Strand-specific TruSeq RNA-seq library prep (Illumina)
attributes.tag: ENA first public
attributes.value: 2016-12-12
attributes.tag: ENA last update
attributes.value: 2016-11-30
attributes.tag: External Id
attributes.value: SAMEA18180418
attributes.tag: INSDC center alias
attributes.value: Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences
attributes.tag: INSDC center name
attributes.value: Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences
attributes.tag: INSDC first public
attributes.value: 2016-12-12T11:05:38Z
attributes.tag: INSDC last update
attributes.value: 2016-11-30T15:57:24Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-5300:BrDi.W4.mix.1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: stiff brome
attributes.tag: day length
attributes.value: 8
attributes.tag: genotype
attributes.value: line BD1-1
attributes.tag: individual
attributes.value: mix
attributes.tag: light intensity
attributes.value: 50
attributes.tag: organism part
attributes.value: leaf
attributes.tag: sample name
attributes.value: E-MTAB-5300:BrDi.W4.mix.1
attributes.tag: temperature
attributes.value: 6