title: CHRF
organism: Homo sapiens
description: Protocols: CHRF 288-11 cells were cultured in RPMI-1640 (Sigma-Aldrich, Gillingham, UK, R0883), 10 % FBS (Invitrogen, Paisley, UK, 10100-147) and 200 uM L-glutamine, 100 U penicillin and 100 ug/ml streptomycin (Sigma-Aldrich, G1146). We performed FAIRE in the human megakaryocytic cell line CHRF-288-11. Cells were maintained in RPMI-1640 medium (Sigma-Aldrich, R0883), 20 % horse serum (heat- inactivated, Invitrogen, 26050-088), and 1 % L-glutamine-penicillin-streptomycin solution (Sigma-Aldrich, G1146), and grown at 37C, 5 % CO2, and 100% humidified atmosphere. FAIRE experiments were carried out as previously described. Twenty million cells were cross-linked with 1 % formaldehyde for 12 min and subjected to 12 sonication cycles (30 sec of high pulse, 30 sec of rest) using the Bioruptor UCD-200. A DNA amplification step was neglected. The sample was cleaned-up using the QIAGEN MinElute PCR Purification Kit (Qiagen, 28004). FAIRE DNA was processed following the Illumina paired-end library generation protocol.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2012-01-11T17:00:36Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T15:35:51Z
attributes.tag: External Id
attributes.value: SAMEA1094711
attributes.tag: INSDC center name
attributes.value: SC
attributes.tag: INSDC first public
attributes.value: 2012-01-11T17:00:36Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T15:35:51Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-858:CHRF
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: CHRF-288-11
attributes.tag: common name
attributes.value: human
attributes.tag: disease state
attributes.value: megakaryoblastic leukaemia
attributes.tag: individual
attributes.value: CHRF
attributes.tag: sample name
attributes.value: E-MTAB-858:CHRF
attributes.tag: scientific_name
attributes.value: Homo sapiens