title: Sample_17A
organism: Arabidopsis thaliana
description: graft of shoot/root C24/C24 Protocols: Plants of Arabidopsis thaliana accessions Col-0 and C24 were utilized in our experiments, as well as a previously described dcl2-1/dcl3-1/dcl4-2 triple mutant (Col-0 background). The C24 plants harbored a GFP transgene silenced by a partial GFP inverted-repeat, termed GxGF-IR. Arabidopsis plants were grown under 10 h supplemental fluorescent lighting at 20ºC, on vertical plates of 0.8% agar, 1⁄2 Murashige and Skoog media, pH 5.7. Micrografting was conducted seven days after germination.Plant tissue was harvested five weeks after grafting, taking care to separate root from scion and exclude tissue 0.3-0.5 cm surrounding the graft junction. DNA for the MethylC-seq libraries was prepared from 3-4 pooled plants per sample using the Gentra Puregene Tissue Kit (Qiagen) according to manufacturer's instructions. MethylC-seq libraries were constructed as described previously (Schmitz et al, Science 334, 369-373).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2016-01-04T17:01:46Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-09T01:22:58Z
attributes.tag: External Id
attributes.value: SAMEA3432729
attributes.tag: INSDC center name
attributes.value: University of Cambridge
attributes.tag: INSDC first public
attributes.value: 2016-01-04T17:01:46Z
attributes.tag: INSDC last update
attributes.value: 2018-03-09T01:22:58Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-3473:Sample_17A
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: thale cress
attributes.tag: ecotype
attributes.value: C24/C24
attributes.tag: organism part
attributes.value: root structure
attributes.tag: sample name
attributes.value: E-MTAB-3473:Sample_17A
attributes.tag: scientific_name
attributes.value: Arabidopsis thaliana