title: Wild type Switch B
organism: Herbaspirillum seropedicae SmR1
description: Protocols: Herbaspirillum seropedicae strains were grown at 30°C in NFbHP-Malate medium supplemented with 20 mM of NH4Cl under 350 rpm (high oxygen) or upon switch from 350 rpm to 120 rpm (low oxygen) Total RNA was purified using the Qiagen Rneasy Mini Kit (#74104) according to manufacturer instructions. mRNA enrichment was performed using Ribo-Zero Gram-negative Bacteria kit (Epicentre#MRZGN126) according to manufacturer instructions The library was constructed using the Illumina TruSeq RNA library kit by the sequencing contractor.
attributes.tag: ENA first public
attributes.value: 2018-02-26
attributes.tag: ENA last update
attributes.value: 2018-01-18
attributes.tag: External Id
attributes.value: SAMEA104483586
attributes.tag: INSDC center alias
attributes.value: Postdoctoral Researcher
attributes.tag: INSDC center name
attributes.value: Postdoctoral Researcher
attributes.tag: INSDC first public
attributes.value: 2018-02-26T17:02:53Z
attributes.tag: INSDC last update
attributes.value: 2018-01-18T11:46:04Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-5741:Wild type Switch B
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: growth condition
attributes.value: 120 rpm - switch or low oxygen
attributes.tag: sample name
attributes.value: E-MTAB-5741:Wild type Switch B
attributes.tag: strain
attributes.value: SmR1