title: Aphidicolin+ATRi E14 single cell
organism: Mus musculus
description: Aphidicolin+ATRi treated E14 cells.
attributes.tag: strain
attributes.value: not applicable
attributes.tag: age
attributes.value: not applicable
attributes.tag: dev_stage
attributes.value: not applicable
attributes.tag: sex
attributes.value: male
attributes.tag: tissue
attributes.value: cell culture
attributes.tag: cell_line
attributes.value: E14
attributes.tag: growth_protocol
attributes.value: ESCs were grown on feeder free culture condition and incubated at 37¡C and 3% O2 tension. ESCs medium (knockOut DMEM, Thermo fisher), 15% ESC qualified Fetal Bovine Serum (Thermo fisher), 2 mM L-glutamine, 1/500 home-made leukemia inhibitory factor, 0,1 mM non-essential amino acids, 0,1 mM 2-Bmercaptoethanol, 50 units/ml penicillin, 50 mg/ml streptomycin supplemented with the two inhibitors PD 0325901 (1uM) and CHIR 99021 (3uM).
attributes.tag: sample_type
attributes.value: cell culture
attributes.tag: treatment
attributes.value: Aphidicolin 6uM + ATRi (VE 822) 1uM
attributes.tag: BioSampleModel
attributes.value: Model organism or animal