title: SGBS D14
organism: Homo sapiens
attributes.tag: source_name
attributes.value: human mature adipocyte
attributes.tag: cell line
attributes.value: SGBS
attributes.tag: cell type
attributes.value: adipocyte
attributes.tag: passages
attributes.value: 42-46
attributes.tag: differentiation days
attributes.value: 14
attributes.tag: culture media
attributes.value: DMEM/F12 supplemented with transferrin (0.01 mg/ml), insulin (20nM), cortisol (100nM), and triiodothyronine (T3, 0.2 nM). For the initial four days, the differentiation medium was additionally supplemented with dexamethasone (25nM), IBMX (250 μM) and rosiglitazone (2 μM).