title: Hyposoter didymator ovaries pupae stage 3 M_S21
organism: Hyposoter didymator
description: Rearing of Hyposoter didymator wasps was conducted as described by Visconti et al.(Molecular Immunology, 2019). Total RNAs were extracted from pools of 40 to 50 ovaries dissected from females at pupal stage 3 (~5 day-old pupae) using the Qiagen RNeasy extraction kit according to manufacturer's protocol. Following extraction, total RNAs were systematically submitted to DNase treatment with the TURBO DNA-free Kit (Life Technologies).
attributes.tag: replicate
attributes.value: Pupae stage 3 biological replicate M
attributes.tag: strain
attributes.value: missing
attributes.tag: isolate
attributes.value: missing
attributes.tag: breed
attributes.value: missing
attributes.tag: cultivar
attributes.value: missing
attributes.tag: ecotype
attributes.value: missing
attributes.tag: dev_stage
attributes.value: Pupae stage 3
attributes.tag: sex
attributes.value: female
attributes.tag: tissue
attributes.value: ovaries
attributes.tag: biomaterial_provider
attributes.value: Anne-Nathalie VOLKOFF (DGIMI, INRA, France)
attributes.tag: BioSampleModel
attributes.value: Model organism or animal