organism: Homo sapiens
description: Use HBB-mut-F2+HBB-mut-R1 to amplify the endogenous HBB sequence
attributes.tag: isolate
attributes.value: 37
attributes.tag: age
attributes.value: not applicable
attributes.tag: biomaterial_provider
attributes.value: Baisong Lu
attributes.tag: sex
attributes.value: not applicable
attributes.tag: tissue
attributes.value: Human embryo kidney
attributes.tag: cell_line
attributes.value: HEK293T
attributes.tag: sample_type
attributes.value: To detect background HBB Indels
attributes.tag: treatment
attributes.value: 293T cells treated with lentiviral vector only expressing HBB sgRNA1, but without Cas9
attributes.tag: BioSampleModel
attributes.value: Human