title: ZR75.1_ER-beta
organism: Homo sapiens
description: Protocols: Human breast cancer ZR75.1 cells were used to produce stable clones expressing ER-beta tagged with 3xFlag-tag respectively at the N-term (ZR-flag-ER-beta). All were grown in Dulbeccos modified Eagles medium (DMEM), supplemented with 10% fetal bovine serum (FBS) (both from Invitrogen SpA, San Giuliano, Milanese, Italy), 100 U/ml penicillin, 100 mg/ml streptomycin, 250 ng/ml Amfotericin-B, 50 ug/ml G418 (normal growing condition). For G0-G1 synchronization cells were plated at 40% confluence in steroid-free medium (phenol red-free DMEM medium, with 5% fetal bovine serum pre-treated with dextran-coated charcoal and antibiotics) and maintained for 5 days without any additional scompound Cells were harvested in absence of estrogen stimuli Total RNA was extracted using TRIZOL reagent (Invitrogen) according to the manufacturer instructions. 1ug of total RNA was used in a library preparation according to the Illumina TruSeq small RNA sample preparation protocol rev. E (Illumina, USA).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2014-11-13T17:02:20Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T16:54:55Z
attributes.tag: External Id
attributes.value: SAMEA2250476
attributes.tag: INSDC center name
attributes.value: Laboratory of Molecular Medicine and Genomics, Department of Medicine and Surgery, University of Salerno, Baronissi, Italy.
attributes.tag: INSDC first public
attributes.value: 2014-11-13T17:02:20Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T16:54:55Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-2069:ZR75.1_ER-beta
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: ZR75.1
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: breast cancer
attributes.tag: genotype
attributes.value: ER-beta expression
attributes.tag: sample name
attributes.value: E-MTAB-2069:ZR75.1_ER-beta
attributes.tag: scientific_name
attributes.value: Homo sapiens