title: Hoxa2_exp2
organism: Mus musculus
description: Protocols: The preparation of the material was as described in Kutejova et al., 2008. Branchial arches are dissected (the whole tissue), crosslinked and frozen in order to pool enough for ChIP. Kutejova E, Engist B, Self M, Oliver G, Kirilenko P, Bobola N. Six2 functions redundantly immediately downstream of Hoxa2. Development. 2008. 135(8):1463-70. PMID: 18321982. ChIP assays were performed as described in Kutejova et al., 2008, with the following modifications for ChIP-seq. The crosslinked material was sonicated to 300 bp fragments (Vibracell sonicator, Sonics: 8 times for 10 seconds at 50% output). Immunoprecipitation was performed starting from 12 pairs of second branchial arches from E11.5 embryos and incubating overnight at 4oC with 3 ug of anti-Hoxa2 antibodies or control IgG (Kutejova et al., 2008), followed by protein A beads (Invitrogen). Kutejova E, Engist B, Self M, Oliver G, Kirilenko P, Bobola N. Six2 functions redundantly immediately downstream of Hoxa2. Development. 2008. 135(8):1463-70. PMID: 18321982.
attributes.tag: ENA first public
attributes.value: 2012-01-17
attributes.tag: ENA last update
attributes.value: 2018-03-08
attributes.tag: External Id
attributes.value: SAMEA1031352
attributes.tag: INSDC center alias
attributes.value: UNIVERSITY OF MANCHESTER
attributes.tag: INSDC center name
attributes.value: UNIVERSITY OF MANCHESTER
attributes.tag: INSDC first public
attributes.value: 2012-01-17T11:10:22Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T15:27:24Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-711:Hoxa2_exp2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: second branchial arch
attributes.tag: common name
attributes.value: house mouse
attributes.tag: sample name
attributes.value: E-MTAB-711:Hoxa2_exp2
attributes.tag: sex
attributes.value: mixed sex