title: Illumina RNA-seq results of Eustigmatos cf. polyphem
organism: Vischeria cf. polyphem
description: Eustigmatos cf. polyphem is formarly determined as Myrmecia bisecta, which Taxon ID is 41462.   Total RNA was isolated using TRIzol reagent (Invitrogen) according to the manufacturer's protocol from pure axenic cultures of E. cf. polyphem.The samples for transcriptome analysis were prepared using Illumina's kit following manufacturer's recommendations. Briefly, mRNA was purified from 6 μg of total RNA using oligo (dT) magnetic beads. Following purification, mRNA is fragmented into small pieces using divalent cations under elevated temperature and the cleaved RNA fragments were used for first strand cDNA synthesis using reverse transcriptase and random primers. This was followed by second strand cDNA synthesis using DNA polymerase I and RNaseH. These cDNA fragments then went through an end repair process and ligation of adapters. These products were purified and enriched with PCR to create the final cDNA library.At last, the library was sequenced using Illumina GA IIX.