title: Metagenomic sequencing of KB-1 microbial consotrium: KB-1/VC-H2 strain KBVC1
organism: Dehalococcoides mccartyi
description: Metagenomic sequencing of KB-1: a mixed microbial consotrium used to enhance bioremediation of chlorinated solvents in subsurface environments. This series of cultures were originally enriched from contaminated sediment from Southern Ontario in 1996. Subsequent transfers in 1997 were amended with vinyl chloride (VC) and 5x electron equivalents of methanol (MeOH). The culture was further enriched using 2% transfers in 2001 and grown on VC and 5x electron equivalents of hydrogen gas. Formally named KB-1/VC-H2 after its lineage, electron acceptor and donor. This culture continues to be maintained batch style in anaerobic minimal medium in the Edwards Laboratory at the University of Toronto.
attributes.tag: strain
attributes.value: KBVC1
attributes.tag: num_replicons
attributes.value: missing
attributes.tag: rel_to_oxygen
attributes.value: anaerobe
attributes.tag: isol_growth_condt
attributes.value: missing
attributes.tag: env_material
attributes.value: water
attributes.tag: lat_lon
attributes.value: 43.6587 N 79.3955 W
attributes.tag: env_biome
attributes.value: bioreactor
attributes.tag: ref_biomaterial
attributes.value: primary publication
attributes.tag: depth
attributes.value: groundwater
attributes.tag: env_feature
attributes.value: anaerobic bioreactor
attributes.tag: geo_loc_name
attributes.value: Canada: Toronto
attributes.tag: collection_date
attributes.value: 2013-02
attributes.tag: BioSampleModel
attributes.value: MIGS.ba
attributes.tag: BioSampleModel
attributes.value: MIGS/MIMS/MIMARKS.water