title: Sample 5
organism: Mus musculus
description: Protocols: Colonic epithelial cells were dissociated in HBSS containing 5mM EDTA and 10mM HEPES. Cells were further digested using Dispase (5 units per ml; BD Biosciences ), CollagenaseD (0.5 mg/ml; Roche) and DNaseA (0.5 mg/ml; Sigma-Aldrich). Cells were highly purified as DAPI-EpCam+CD45-CD24MedLgr5+ For γ-irradiation of mice, animals were exposed to a Caesium137 source in a OB58/905-2 irradiator (Buchler, Germany). total RNA was isolated using Trizol according to the Immgen protocol (https://www.immgen.org/Protocols/Total%20RNA%20Extraction%20with%20Trizol.pdf) SMARTer Ultra Low Input RNA (Clontech) was used for library preparation
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-01-01T17:01:56Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-04-27T14:08:20Z
attributes.tag: External Id
attributes.value: SAMEA4618624
attributes.tag: INSDC center name
attributes.value: Institute of Microbiology and Infection Immunology, Charite - University Medicine Berlin, 12203 Berlin, Germany
attributes.tag: INSDC first public
attributes.value: 2019-01-01T17:01:56Z
attributes.tag: INSDC last update
attributes.value: 2018-04-27T14:08:20Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6723:Sample 5
attributes.tag: age
attributes.value: 8 to 10
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: epithelial cell of large intestine
attributes.tag: common name
attributes.value: house mouse
attributes.tag: genotype
attributes.value: Lgr5-Cre-ERT2
attributes.tag: organism part
attributes.value: colon
attributes.tag: phenotype
attributes.value: DAPI-EpCam+CD45-CD24MedLgr5+
attributes.tag: sample name
attributes.value: E-MTAB-6723:Sample 5
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: strain
attributes.value: C57BL/6