title: raw_rbm20_hits_NH_HM_004
organism: Rattus norvegicus
description: Protocols: Neonatal rat ventricular myocytes (NRVMs) were isolated from SD rats using a modification of the Kasten's technique as described (37). 48 h past isolation, cardiomyocytes were irradiated one time for 400mJ/cm and additional 200 mJ/cm with 254 nm UV light, and lysed in NP40 lysis buffer. After DNAse and RNase T1 treatment, the lysate was immunoprecipitated using RBM20 antibody (5) covalently coupled to Protein G Dynabeads (Invitrogen). Radiolabeling of immunoprecipitated RNA, protein-RNA complexes separation, sequencing library preparation and sequencing was done following the PAR-CLIP protocol 4 ug of total RNA was used for library preparation with the TruSeq RNA Sample Preparation Kit (Illumina).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2014-11-11T17:02:10Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T20:51:07Z
attributes.tag: External Id
attributes.value: SAMEA3112878
attributes.tag: INSDC center name
attributes.value: MDC
attributes.tag: INSDC first public
attributes.value: 2014-11-11T17:02:10Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T20:51:07Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-2572:raw_rbm20_hits_NH_HM_004
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: myocyte
attributes.tag: common name
attributes.value: Norway rat
attributes.tag: individual
attributes.value: HITS-CLIP 3
attributes.tag: sample name
attributes.value: E-MTAB-2572:raw_rbm20_hits_NH_HM_004
attributes.tag: scientific_name
attributes.value: Rattus norvegicus
attributes.tag: specimen with known storage state
attributes.value: frozen specimen