title: 3_KO_male
organism: Mus musculus
description: Protocols: Two days post-natal Bicc1 WT and KO mice were sacrificed by decapitation. For RNAseq experiment one kidney per animal was collected, flash frozen in liquid nitrogen and stored at -80°C. The contralateral kidneys of the same animals were collected and processed according to the ChIPseq protocol. The total RNA (from 14 kidneys) was extracted using a Qiagen RNAeasy plus mini kit and a TRIzol/Chloroform extraction procedure. The RNA concentration and quality was determined using a Thermo Scientific™ NanoDrop™ spectrophotometer (1.8 ≤ A260/A280 ≤ 2.2). For each genotype (WT, KO) and gender (male and female), pools of equal RNA amounts from two total RNA samples were generated. A total of seven pools resulting in two replicates per genotype and sex (with the exception of knockout females: only two animals were available resulting in only one pool) were used for mRNA library preparation. Illumina TruSeq Stranded mRNA protocol (Illumina, USA) was followed using 500 ng of RNA per pool-sample as starting material. Libraries were checked for quality and quantified using the Bioanalyzer 2100 (Agilent, USA) before being sequenced.
attributes.tag: ENA first public
attributes.value: 2019-01-20
attributes.tag: ENA last update
attributes.value: 2018-01-24
attributes.tag: External Id
attributes.value: SAMEA104547407
attributes.tag: INSDC center alias
attributes.value: Prof. Daniel Constam' s group (UPCDA) Ecole Polytechnique Federale de Lausanne (EPFL) SV ISREC, Lausanne, Switzerland.
attributes.tag: INSDC center name
attributes.value: Prof. Daniel Constam' s group (UPCDA) Ecole Polytechnique Federale de Lausanne (EPFL) SV ISREC, Lausanne, Switzerland.
attributes.tag: INSDC first public
attributes.value: 2019-01-20T17:02:07Z
attributes.tag: INSDC last update
attributes.value: 2018-01-24T15:17:59Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6423:3_KO_male
attributes.tag: age
attributes.value: 2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: diet
attributes.value: 2 days post natal mice : mother milk ; adult (mother) mice : Breeding diet 3242 from KLIBA NAFAG, PROVIMI KLIBA AG
attributes.tag: disease
attributes.value: polycystic kidney disease
attributes.tag: genotype
attributes.value: Bicc1 homozygous knockout
attributes.tag: organism part
attributes.value: kidney
attributes.tag: phenotype
attributes.value: cystic kidney
attributes.tag: sample name
attributes.value: E-MTAB-6423:3_KO_male
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: C57BL/6