title: sample preparation for a normalized cDNA library of heart and liver RNA of Pachycara brachycephalum
organism: Pachycara brachycephalum
description: Specimens of P. brachycephalum were caught with baited traps during an expedition ANTXV/3 with the RV "Polarstern" close to the Antarctic Peninsula. The animals were brought to the Alfred Wegener Institute in Bremerhaven and kept under habitat conditions until experimentation. Preparation of a random-primed and normalized cDNA for pyrosequencing was done with pooled total RNA from liver and heart. For further information please refer to Windisch et al 2012.