title: Kuenenia stuttgartiensis anoxic membrane bioreactor enrichment culture
organism: Candidatus Kuenenia stuttgartensis
description: Planktonic Kuenenia cells sustained in a anoxic membrane bioreactor. The culture was obtained by Strous et. al (2006) by inoculating a gas lift bioreactor with nitrifying activated sludge in 2002. This resulted in a enrichment of Kuenenia cells occurring as flocs. The culture was transferred into a sequencing batch reactor in 2004. The current culturing setup, in operation since 2009, utilizes a membrane bioreactor. This change in cultivation enabled obtaining planktonic cells instead of flocs which resulted in an enrichment of up to 95%.
attributes.tag: ENA first public
attributes.value: 2017-10-20
attributes.tag: ENA last update
attributes.value: 2017-10-02
attributes.tag: External Id
attributes.value: SAMEA104314680
attributes.tag: INSDC center alias
attributes.value: RU
attributes.tag: INSDC center name
attributes.value: Radboud University Nijmegen, The Netherlands
attributes.tag: INSDC first public
attributes.value: 2017-10-20T17:02:48Z
attributes.tag: INSDC last update
attributes.value: 2017-10-02T22:07:11Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: KS_RU-NIJMEGEN_1
attributes.tag: collection date
attributes.value: 2014-03
attributes.tag: environment (biome)
attributes.value: anaerobic bioreactor
attributes.tag: environment (feature)
attributes.value: anaerobic bioreactor
attributes.tag: environment (material)
attributes.value: anaerobic bioreactor
attributes.tag: geographic location (country and/or sea)
attributes.value: Netherlands
attributes.tag: geographic location (latitude)
attributes.value: 51.8238509
attributes.tag: geographic location (longitude)
attributes.value: 5.8685827
attributes.tag: investigation type
attributes.value: metagenome
attributes.tag: miscellaneous environmental package
attributes.value: miscellaneous natural or artificial environment
attributes.tag: project name
attributes.value: Resolving the complete genome of Kuenenia stuttgartiensis from a membrane bioreactor enrichment using Single Molecule Real-Time sequencing
attributes.tag: sample name
attributes.value: KS_RU-NIJMEGEN_1
attributes.tag: sequencing method
attributes.value: Single Molecule Real-Time sequencing - PacBio RS II