title: MB49
organism: Mus musculus
description: Protocols: Cells were grown in DMEM supplemented with 5% fetal calf serum, 2mM glutamine and 5µg/mL gentamycin. 24h before collection, cells were transferred in plates coated with polyHEMA. At harvest time, supernatants were collected and centrifuged 5min à 400g. Cell pellets were used for nucleid acid extraction. Cells were lysed in TRI reagent (MRC) and total RNA isolated according to the manufacturer's instructions, using chloroform for the phase separation. RNA quality was assessed by spectrophotometry (NanoDrop™ 2000c, Thermo Scientific). Sequencing libraries were generated using NEBNext Ultra II Directional RNA preparation kit following manufacturer's recommendations.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-09-20T04:05:04Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-09-10T16:37:02Z
attributes.tag: External Id
attributes.value: SAMEA7301470
attributes.tag: INSDC center name
attributes.value: CNRS, UMR 9018, Gustave Roussy and Universite Paris-Saclay
attributes.tag: INSDC first public
attributes.value: 2020-09-20T04:05:04Z
attributes.tag: INSDC last update
attributes.value: 2020-09-10T16:37:02Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-9570:MB49
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: MB49
attributes.tag: cell type
attributes.value: epithelial cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: bladder carcinoma
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: urinary bladder
attributes.tag: sample name
attributes.value: E-MTAB-9570:MB49
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: strain
attributes.value: C57BL/6NJ