title: Bisulfite sequencing of Zea mays B73 early-prophase meiocytes
organism: Zea mays
description: Bisulfite sequencing of Zea mays B73 early-prophase meiocytes, anthers and seedling control. We used isolated zygotene meiocytes from the Zea mays inbred line B73 and 2-week-old seedlings for chromatin extraction and subsequently performed bisulfite conversion and library construction for sequencing with Illumina technology. ~20,000 meiocytes and ~24 anthers were collected from 8 B73 maize plants, and for the seedling sample 3 2-week old B73 maize seedlings were pooled.
attributes.tag: cultivar
attributes.value: B73
attributes.tag: dev_stage
attributes.value: zygotene
attributes.tag: geo_loc_name
attributes.value: USA: UMN Greenhouse St. Paul MN
attributes.tag: tissue
attributes.value: Meiocytes
attributes.tag: BioSampleModel
attributes.value: Plant