title: MDCKTGFbD6_3
organism: Canis lupus familiaris
description: Protocols: MDCK cells were maintained in DMEM growth medium supplemented with 10% Foetal Bovine Serum (FBS) For TGF-beta treatment, wildtype MDCK were stimulated in culture by adding 5ng of TGF-beta to the medium. TGF-beta was added with every passage of cells, to maintain stimulus. Total RNA was extracted usign Qiagen RNAeasy kit following manufacturer's instructions. Libraries were constructed according to the Illumina TruSeq stranded mRNA protocol with poly-A enrichment.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2017-10-23T17:03:32Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2017-03-28T11:43:27Z
attributes.tag: External Id
attributes.value: SAMEA103942076
attributes.tag: INSDC center name
attributes.value: Research Fellow Tremethick Lab Department of Genome Sciences The John Curtin School of Medical Research The Australian National University
attributes.tag: INSDC first public
attributes.value: 2017-10-23T17:03:32Z
attributes.tag: INSDC last update
attributes.value: 2017-03-28T11:43:27Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-5628:MDCKTGFbD6_3
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: MDCK
attributes.tag: cell type
attributes.value: Epithelial cell of kidney
attributes.tag: common name
attributes.value: dog
attributes.tag: growth condition
attributes.value: MDCK cells were maintained in DMEM growth medium supplemented with 10% Foetal Bovine Serum (FBS)
attributes.tag: organsim part
attributes.value: kidney
attributes.tag: sample name
attributes.value: E-MTAB-5628:MDCKTGFbD6_3
attributes.tag: scientific_name
attributes.value: Canis lupus familiaris
attributes.tag: sex
attributes.value: female
attributes.tag: sub_species
attributes.value: familiaris