title: Chicken gut microbiota determines Salmonella colonization
organism: Gallus gallus
description: Heterogeneity of infection and extreme shedding patterns are common features of animal infectious diseases. The super-shedder host individuals are considered key targets for control strategies. Nevertheless, the mechanisms, dynamics, and patterns of diversity associated with the emergence of super-shedders remain largely unknown. For chicken salmonellosis, we have shown recently that high heterogeneity of infection is observed in isolator, an experimental device allowing to reduce animal-animal cross-contaminations and reinfections. With this device, we suggested that unlike the super-shedders, the low-shedders are able to block the first Salmonella colonization. In the present paper, we demonstrated that (1) chicks either bred in axenic conditions or harbouring depleted gut microbiota are more prone to become super-shedders; (2) it is possible to acquire the super or low-shedder phenotypes through microbiota transfer; (3) the chicks bred in isolator, which will develop the low-shedder phenotype after Salmonella infection, present specific gut microbiota taxonomic features before infection. These observations show the key role of gut microbiota composition in heterogeneity of infection and pave the way of developing predictive biomarkers and protective probiotics.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-08-20T17:04:17Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-05-21T13:12:53Z
attributes.tag: External Id
attributes.value: SAMEA5624905
attributes.tag: INSDC center name
attributes.value: INRA DE TOURS
attributes.tag: INSDC first public
attributes.value: 2019-08-20T17:04:17Z
attributes.tag: INSDC last update
attributes.value: 2019-05-21T13:12:53Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: FPP3
attributes.tag: adapters
attributes.value: CCTACGGGNGGCWGCAG
attributes.tag: collection date
attributes.value: 2015-06-01/2018-02-01
attributes.tag: common name
attributes.value: chicken
attributes.tag: environment (biome)
attributes.value: temperate
attributes.tag: environment (feature)
attributes.value: building
attributes.tag: environment (material)
attributes.value: air
attributes.tag: geographic location (country and/or sea)
attributes.value: France
attributes.tag: geographic location (latitude)
attributes.value: 47.5434� N
attributes.tag: geographic location (longitude)
attributes.value: 0.7442� E
attributes.tag: host age
attributes.value: 11 days
attributes.tag: host body habitat
attributes.value: intestine
attributes.tag: host body product
attributes.value: stool
attributes.tag: host body site
attributes.value: intestine
attributes.tag: host common name
attributes.value: chicken
attributes.tag: host growth conditions
attributes.value: isolator reducing host-to-host cross contaminations
attributes.tag: host subject id
attributes.value: 21
attributes.tag: host taxid
attributes.value: 9030
attributes.tag: host-associated environmental package
attributes.value: host-associated
attributes.tag: investigation type
attributes.value: metagenome
attributes.tag: known pathogenicity
attributes.value: inoculated with Salmonella
attributes.tag: multiplex identifiers
attributes.value: TCGATGT
attributes.tag: nucleic acid amplification
attributes.value: doi: 10.1093/nar/gks808
attributes.tag: pcr primers
attributes.value: S-D-Bact-0341-b-S-17 / S-D-Bact-0785-a-A-21
attributes.tag: project name
attributes.value: The transnational Aniwha project AWAP and the joint research program MoMIR-PPC (One Health European Joint Program)
attributes.tag: sample collection device or method
attributes.value: individual fecal sampling in isolator
attributes.tag: sample name
attributes.value: FPP3
attributes.tag: scientific_name
attributes.value: Gallus gallus
attributes.tag: sequencing method
attributes.value: Illumina Miseq
attributes.tag: target gene
attributes.value: 16S V3V4 region