title: Sample 3
organism: Rattus norvegicus
description: Protocols: Neonatal rat ventricular cardiomyocytes were treated with either control or Vezf1 siRNA for 72 hours. Cell samples were washed twice with PBS and snap frozen at -80 deg. celcius Control Total RNA was collected using EZNA Total RNA kit I (Omega Bio-tek, Norcross, GA) Nuclease-Free water (Thermo Fisher Scientific, Waltham, MA) was used to dissolve the RNA pellets or eluate the RNA from isolation columns For cDNA synthesis Transcriptor 1st strand cDNA synthesis kit (Roche, Basel, Switzerland) was used according to manufacturer's instructions.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-08-19T04:02:51Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-08-09T13:02:38Z
attributes.tag: External Id
attributes.value: SAMEA5859691
attributes.tag: INSDC center name
attributes.value: University of Oulu Institute of Biomedicine
attributes.tag: INSDC first public
attributes.value: 2019-08-19T04:02:51Z
attributes.tag: INSDC last update
attributes.value: 2019-08-09T13:02:38Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7124:Sample 3
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: cardiac muscle cell
attributes.tag: common name
attributes.value: Norway rat
attributes.tag: developmental stage
attributes.value: neonate
attributes.tag: organism part
attributes.value: cardiac ventricle
attributes.tag: sample name
attributes.value: E-MTAB-7124:Sample 3
attributes.tag: scientific_name
attributes.value: Rattus norvegicus
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: Sprague-Dawley