title: WT105
organism: Mus musculus
description: Protocols: Cells were grown in RPMI supplemented with 10% fetal calf serum, 2mM glutamine and 5µg/mL gentamycin. 24h before collection, cells were transferred in low attachment plates (Corning®). At harvest time, supernatants were collected and centrifuged 5min at 400g. Cell pellets were used for nucleic acid extraction. Cells were lysed in TRI reagent (MRC) and total RNA isolated according to the manufacturer's instructions, using chloroform for the phase separation. RNA quality was assessed by spectrophotometry (NanoDrop™ 2000c, Thermo Scientific). Sequencing libraries were generated using NEBNext Ultra II Directional RNA preparation kit following manufacturer's recommendations.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-09-20T04:05:04Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-09-10T16:29:34Z
attributes.tag: External Id
attributes.value: SAMEA7301465
attributes.tag: INSDC center name
attributes.value: CNRS, UMR 9018, Gustave Roussy and Universite Paris-Saclay
attributes.tag: INSDC first public
attributes.value: 2020-09-20T04:05:04Z
attributes.tag: INSDC last update
attributes.value: 2020-09-10T16:29:34Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-9559:WT105
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: CT26
attributes.tag: cell type
attributes.value: intestinal epithelial cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: colon carcinoma
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: colon
attributes.tag: sample name
attributes.value: E-MTAB-9559:WT105
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: strain
attributes.value: BALB/c