title: Setd5 ES cells KO; 3586-MAM-5
organism: Mus musculus
description: Protocols: Heterozygous (Het) and knockout (KO) Setd5 mouse embryonic stem cell (mESC) lines were derived from inner cells mass outgrowths obtained from blastocysts cultured in mouse ES cell media (Dulbecco's modified Eagle's medium supplemented with 15% FBS, 1X nonessential amino acids, 1X penicillin-streptomycin, 1× L-glutamine containing MEK1 inhibitor PD98059 (Cell Signaling). All mES cells were cultured on a feeder layer of mitotically inactivated mouse embryonic fibroblasts (MEFs) in mES cell culture medium supplemented with 1000 U/ml of leukemia inhibitory factor (ESGRO/LIF, Millipore) according to standard protocols. For RNA isolations, mES cells were re-plated on gelatinased plates without MEFs. Total RNA was extracted from mES cells using TRIzol reagent isolation method (Invitrogen). The resulting RNA was treated with 4U of DNAse I (RNase free) (Ambion) for 30 min and purified using DNAse-free RNA kit (Zymo-Research). cDNA libraries were constructed using the TruSeq Stranded mRNA Library Prep Kit (Illumina).
attributes.tag: ENA first public
attributes.value: 2016-12-13
attributes.tag: ENA last update
attributes.value: 2016-10-26
attributes.tag: External Id
attributes.value: SAMEA4523679
attributes.tag: INSDC center alias
attributes.value: Vanderbilt University
attributes.tag: INSDC center name
attributes.value: Vanderbilt University
attributes.tag: INSDC first public
attributes.value: 2016-12-13T17:04:00Z
attributes.tag: INSDC last update
attributes.value: 2016-10-26T10:21:48Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-5189:Setd5 ES cells KO; 3586-MAM-5
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: embryonic stem cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: genotype
attributes.value: Setd5 homozygous (null) knockout
attributes.tag: sample name
attributes.value: E-MTAB-5189:Setd5 ES cells KO; 3586-MAM-5
attributes.tag: specimen with known storage state
attributes.value: fresh specimen