title: Single Amplified Genome TOSAG23-3_ABAI_cell1
organism: Stramenopiles sp. TOSAG23-3
description: This cell was isolated using flow cytometry from a water sample (TARA_X000001437) collected during the Tara Oceans expedition (2009-2013) at station TARA_023 (latitudeN=42.1735 and longitudeE=17.7252), on date/time=2009-11-18T13:50, using a PUMP (High Volume Peristaltic Pump). The water sample material was collected in the marine biome (ENVO:00000447) from a deep chlorophyll maximum layer (ENVO:01000326) at a depth of 53-57 m. The water sample material was size-fractionated (5-20 micrometres) and prepared on board for later detection and identification of single-cell amplified genomes (SAGs) by sequencing (SEQ) methods. The sampled cell has been identified by pyrosequencing methods and amplified using Multiple Displacement Amplification (MDA).
attributes.tag: Chlorophyll Sensor
attributes.value: 0.0868
attributes.tag: Citation
attributes.value: http://doi.pangaea.de/10.1594/PANGAEA.842197
attributes.tag: ENA first public
attributes.value: 2016-01-04
attributes.tag: ENA last update
attributes.value: 2015-12-03
attributes.tag: Event Date/Time End
attributes.value: 2009-11-18T15:46
attributes.tag: External Id
attributes.value: SAMEA3692432
attributes.tag: INSDC center alias
attributes.value: GSC
attributes.tag: INSDC center name
attributes.value: Genoscope
attributes.tag: INSDC first public
attributes.value: 2016-01-04T17:01:48Z
attributes.tag: INSDC last update
attributes.value: 2015-12-03T10:02:23Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Latitude End
attributes.value: 42.1659
attributes.tag: Longitude End
attributes.value: 17.7285
attributes.tag: Salinity Sensor
attributes.value: 38.395417
attributes.tag: Submitter Id
attributes.value: TARA_BCB_ABAI
attributes.tag: depth
attributes.value: 55
attributes.tag: environment (biome)
attributes.value: marine biome (ENVO:00000447); Westerlies Biome (Longhurst 2007)
attributes.tag: environment (feature)
attributes.value: deep chlorophyll maximum layer (ENVO:01000326)
attributes.tag: environment (material)
attributes.value: particulate matter (ENVO:xxxxxxxx), including plankton (ENVO:xxxxxxxx)
attributes.tag: environmental package
attributes.value: water
attributes.tag: event date/time start
attributes.value: 2009-11-18T13:50
attributes.tag: event label
attributes.value: TARA_20091118T1240Z_023_EVENT_PUMP
attributes.tag: further details
attributes.value: https://ws.pangaea.de/dds-fdp/rest/panquery?datasetDOI=doi.pangaea.de/10.1594/PANGAEA.842197&filterParameter=SampleID&filterValue=TARA_X000001437
attributes.tag: latitude start
attributes.value: 42.1735
attributes.tag: longitude start
attributes.value: 17.7252
attributes.tag: marine region
attributes.value: (MS) Mediterranean Sea [MRGID:1905]
attributes.tag: nitrate sensor
attributes.value: 9999
attributes.tag: oxygen sensor
attributes.value: 224.266917
attributes.tag: project name
attributes.value: Tara Oceans expedition (2009-2013)
attributes.tag: protocol description
attributes.value: This cell was isolated using a MoFlo flow cytometer equipped with a 488 nm laser for excitation. This cell was considered as an heterotrophic protist based on the presence of SYBR Green I fluorescence and the absence of chlorophyll fluorescence. The cell was then lysed using cold KOH and its DNA was amplified using multiple displacement amplification (MDA). Flow-cytometric sorting and whole genome amplification were performed by the Bigelow Laboratory Single Cell Genomics Center.
attributes.tag: protocol label
attributes.value: SCG-SEQ_W5-20
attributes.tag: sample collection device
attributes.value: high volume peristaltic pump [HVP-PUMP]
attributes.tag: sample derived from
attributes.value: ERS905354
attributes.tag: sample name
attributes.value: TARA_BCB_ABAI
attributes.tag: sample status
attributes.value: This version can be used to provide data discovery services
attributes.tag: sampling campaign
attributes.value: TARA_20091111Z
attributes.tag: sampling platform
attributes.value: SV Tara
attributes.tag: sampling station
attributes.value: TARA_023
attributes.tag: size fraction lower threshold
attributes.value: 5
attributes.tag: size fraction upper threshold
attributes.value: 20
attributes.tag: temperature
attributes.value: 15.705783