title: Third instar wandering larva RNA-Seq
organism: Drosophila melanogaster
description: CS flies were reared at room temperature (23-24C) for collection.  Approximately 30 third-instar wandering larva were picked.  Whole body samples were homogenized in 1ml of Trizol (Invitrogen, Carlsbad, California, USA) per 50-100 mg of tissue using a glass-Teflon homogenizer.  RNA was purified according to the suggested manufacturer's protocol (Invitrogen).  RNA was sequenced on the Illumina GA II instrument for 75bp paired-end reads.