title: Single Amplified Genome TOSAG39-1_AMDI_cell4
organism: Bathycoccus sp. TOSAG39-1
description: This cell was isolated using flow cytometry from a water sample (TARA_G000000266) collected during the Tara Oceans expedition (2009-2013) at station TARA_039 (latitudeN=18.5918, longitudeE=66.622) on date/time=2010-03-18T04:27, using a PUMP (High Volume Peristaltic Pump). The water sample material was collected at a depth of 3-7 m, targeting a surface water layer (ENVO:00002042) in the marine biome (ENVO:00000447). The water sample was NOT size-fractionated, preserved on board with 48% Glycine Betaine, and stored in liquid nitrogen for later detection of unicellular eukaryote (protist) whole-genome by pyrosequencing methods, and for later single cell genomics analysis. The sampled cell has been identified by pyrosequencing methods and amplified using Multiple Displacement Amplification (MDA).
attributes.tag: Chlorophyll Sensor
attributes.value: 0.099089
attributes.tag: Citation
attributes.value: tbd
attributes.tag: ENA first public
attributes.value: 2016-01-04
attributes.tag: ENA last update
attributes.value: 2021-10-01
attributes.tag: Event Date/Time End
attributes.value: 2010-03-18T08:32
attributes.tag: External Id
attributes.value: SAMEA3692403
attributes.tag: INSDC center alias
attributes.value: GSC
attributes.tag: INSDC center name
attributes.value: Genoscope
attributes.tag: INSDC first public
attributes.value: 2016-01-04T17:01:48Z
attributes.tag: INSDC last update
attributes.value: 2021-10-01T10:24:26Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Latitude End
attributes.value: 18.5706
attributes.tag: Longitude End
attributes.value: 66.5023
attributes.tag: Salinity Sensor
attributes.value: 36.285367
attributes.tag: Submitter Id
attributes.value: TARA_BCB_AMDI
attributes.tag: depth
attributes.value: 5
attributes.tag: environment (biome)
attributes.value: marine biome (ENVO:00000447)
attributes.tag: environment (feature)
attributes.value: surface water layer (ENVO:00002042)
attributes.tag: environment (material)
attributes.value: saline water (ENVO:00002010), including plankton (ENVO:xxxxxxxx)
attributes.tag: environmental package
attributes.value: water
attributes.tag: event date/time start
attributes.value: 2010-03-18T04:27
attributes.tag: event label
attributes.value: TARA_20100318T0427Z_039_EVENT_PUMP
attributes.tag: further details
attributes.value: tbd
attributes.tag: latitude start
attributes.value: 18.5918
attributes.tag: longitude start
attributes.value: 66.622
attributes.tag: nitrate sensor
attributes.value: -1.415582
attributes.tag: oxygen sensor
attributes.value: 193.3535
attributes.tag: project name
attributes.value: Tara Oceans expedition (2009-2013)
attributes.tag: protocol description
attributes.value: This cell was isolated using a MoFlo flow cytometer equipped with a 488 nm laser for excitation. This cell was considered as an heterotrophic protist based on the presence of SYBR Green I fluorescence and the absence of chlorophyll fluorescence. The cell was then lysed using cold KOH and its DNA was amplified using multiple displacement amplification (MDA). Flow-cytometric sorting and whole genome amplification were performed by the Bigelow Laboratory Single Cell Genomics Center.
attributes.tag: protocol label
attributes.value: PROT_NUC-SCG_W
attributes.tag: sample collection device
attributes.value: PUMP (High Volume Peristaltic Pump)
attributes.tag: sample derived from
attributes.value: ERS488887
attributes.tag: sample name
attributes.value: TARA_BCB_AMDI
attributes.tag: sample status
attributes.value: This version can be used to provide data discovery services
attributes.tag: sampling campaign
attributes.value: TARA_20100309Z
attributes.tag: sampling platform
attributes.value: SV Tara
attributes.tag: sampling station
attributes.value: TARA_039
attributes.tag: temperature
attributes.value: 26.81765