title: Rhizosphere microbial communities were collected by saline washes of coarse roots (diameter 2-4 mm) of maize cultivar 3BT, collected at the flowering stage BBCH 63-65. Total DNA was extracted from the collected cells by “FastDNA SPIN kit for soil” (MP Biomedicals, Illkirch, France). The extraction included two bead beating steps of 45 s at 6.5 m s-1 on a FastPrep-24 system (MP Biomedicals) and additional washing steps of the binding matrix each with 1 ml 5.5 M guanidine thiocyanate (Carl Roth GmbH) until the matrix retained its original color (1 - 3 washes).
organism: rhizosphere metagenome
description: Rhizosphere microbial communities were collected by saline washes of coarse roots (diameter 2-4 mm) of maize cultivar 3BT, collected at the flowering stage BBCH 63-65. Total DNA was extracted from the collected cells by “FastDNA SPIN kit for soil” (MP Biomedicals, Illkirch, France). The extraction included two bead beating steps of 45 s at 6.5 m s-1 on a FastPrep-24 system (MP Biomedicals) and additional washing steps of the binding matrix each with 1 ml 5.5 M guanidine thiocyanate (Carl Roth GmbH) until the matrix retained its original color (1 - 3 washes).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2012-01-10T15:58:44Z
attributes.tag: ENA-KEYWORD
attributes.value: GSC, MIxS
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-09T09:55:25Z
attributes.tag: External Id
attributes.value: SAMEA1094730
attributes.tag: Geographic location (country:region,area)
attributes.value: Germany: Lower Saxony
attributes.tag: INSDC center name
attributes.value: VTI
attributes.tag: INSDC first public
attributes.value: 2012-01-10T15:58:44Z
attributes.tag: INSDC last update
attributes.value: 2018-03-09T09:55:25Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: 3BT_c
attributes.tag: collection date
attributes.value: BBCH 63 - 65; August 2009
attributes.tag: current vegetation
attributes.value: maize culticar 3BT
attributes.tag: depth
attributes.value: 2-15 cm
attributes.tag: enviornmental package
attributes.value: soil
attributes.tag: environment (featurel)
attributes.value: field soil; ENVO:00005755
attributes.tag: environment (material)
attributes.value: rhizosphere; ENVO:00005801
attributes.tag: geographic location (latitude)
attributes.value: 52° N
attributes.tag: geographic location (longitude)
attributes.value: 10° E
attributes.tag: investigation type
attributes.value: MIMARKS
attributes.tag: nucleic acid extraction
attributes.value: “FastDNA SPIN kit for soil” (MP Biomedicals, Illkirch, France)
attributes.tag: pcr conditions
attributes.value: initial denaturation: 95degC_15min; 30 cycles of: denaturation: 94degC_60s, annealing: 50degC_60s, extention: 72degC_70s; final extension at 72degC_5min
attributes.tag: pcr primers
attributes.value: F_Com1_MID11: ccatctcatccctgcgtgtctccgactcagTGATACGTCTCAGCAGCCGCGGTAATAC; R_Com2: cctatcccctgtgtgccttggcagtctcagCCGTCAATTCCTTTGAGTTT
attributes.tag: ph
attributes.value: 6.1
attributes.tag: project name
attributes.value: maize rhizosphere
attributes.tag: sample name
attributes.value: 3BT_c
attributes.tag: scientific_name
attributes.value: rhizosphere metagenome
attributes.tag: sequencing method
attributes.value: Pyrosequencing
attributes.tag: target gene
attributes.value: 16S rRNA