title: Mouse_2_GEX
organism: Mus musculus
description: Protocols: Mice (n=2) were infected with MT P. chabaudi (AS)-GFP by intra-peritoneal injection of 1x105 parasitised erythrocytes, and euthanised at day 7 post-infection. CD3+ CD4+ splenocytes were were isolated by FACS using a BD FACSAria III instrument and sorted into 100L 0.04% BSA in PBS. Standard 10x Genomics protocols for the kit were used to load cells into the Chromium chip. Barcoded cDNA libraries were prepared from sorted samples using the 10x Genomics Single Cell 5' Library Kit v2. Full length V(D)J segments were enriched from amplified cDNA with primers specific to the TCR constant region using the Chromium Single Cell V(D)J Enrichment Kit - Mouse T-Cell. Libraries were prepared as per manufacturers' protocol.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-11-13T04:06:52Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-10-21T17:32:52Z
attributes.tag: External Id
attributes.value: SAMEA7472032
attributes.tag: INSDC center name
attributes.value: Inistitute of Immunology and Infection University of Edinburgh
attributes.tag: INSDC first public
attributes.value: 2020-11-13T04:06:52Z
attributes.tag: INSDC last update
attributes.value: 2020-10-21T17:32:52Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-9691:Mouse_2_GEX
attributes.tag: age
attributes.value: 8 to 10
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: CD4-positive, alpha-beta T cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: individual
attributes.value: Mouse 2
attributes.tag: infect
attributes.value: Plasmodium chabaudi
attributes.tag: organism part
attributes.value: spleen
attributes.tag: sample name
attributes.value: E-MTAB-9691:Mouse_2_GEX
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: C57BL/6J