title: iPS-j
organism: Sus scrofa
description: LIF dependent pig iPS in mouse ESC Protocols: The piPS cells were maintained on mitotically inactive mouse embryonic fibroblasts (MEFs) feeder derived from ICR mice supplemented with 10% FBS, sodium pyruvate, nonessential amino acids, mercaptoethanol, and LIF, bFGF. For immunoprecipitation, the DNA was first denatured, then immunoprecipitated with the 5mC antibody using the Magnetic Methylated DNA Immunoprecipitation kit (Diagenod). Genomic DNA was extracted from the cell pellets using the DNeasy Mini Kit (Qiagen). The gDNA was sonicated to 100-500 bp and repaired to contain a 3-dA overhang; adapters were then ligated to the end of the DNA fragments according to the Paired-End DNA Sample Prep Kit (Illumina). Real time-PCR was performed to validate the enrichment efficiency. Next, the immunoprecipitated DNA was amplified for approximately 12-15 cycles, and fragments of the proper size (usually 200-300 bp) were gel-purified using the Gel Extraction Kit (Qiagen) and quantified using a 2100 Bioanalyzer (Agilent Technologies).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2016-01-06T17:01:52Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T19:42:31Z
attributes.tag: External Id
attributes.value: SAMEA2636343
attributes.tag: INSDC center name
attributes.value: EBI
attributes.tag: INSDC first public
attributes.value: 2016-01-06T17:01:52Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T19:42:31Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-2643:iPS-j
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: induced pluripotent stem cell
attributes.tag: common name
attributes.value: pig
attributes.tag: growth condition
attributes.value: media
attributes.tag: sample name
attributes.value: E-MTAB-2643:iPS-j
attributes.tag: scientific_name
attributes.value: Sus scrofa