title: Seedling-shoot-2-days-light-replicate-3
organism: Brachypodium distachyon
description: Protocols: Samples harvested after growing them in continuous dark for 8 days (8DD) including germination and followed by 48hrs (48LL) of continuous exposure to light (120µmol/m2/sec at soil surface). The growth chamber was set to cycle between 20C for 12 hours and 18C for the next 12 hours, and the soil was kept moist by gently spraying with water every 72 hours. For continuous dark (no light) regimen samples, sampling was done at 7.30 pm after green light. For samples with 8 days continuous dark followed by 2 days continous light, sampling was done at 10am under light. Continuous light (2-Days): Samples harvested after growing them in continuous dark for 8 days including germination followed by 48hrs (48LL) of continuous exposure to light (120µmol/m2/sec at soil surface). Total RNA from frozen seedling shoot samples was extracted as per manufacturer's protocol for RNA Plant reagent (Invitrogen Inc., USA), RNeasy kits (Qiagen Inc., USA), and RNase-free DNase (Life Technologies Inc., USA). True-Seq kit (Illumina Inc.) was used to prepare RNA-seq libraries, according to the manufacturer's protocol.
attributes.tag: ENA first public
attributes.value: 2019-01-10
attributes.tag: ENA last update
attributes.value: 2018-01-31
attributes.tag: External Id
attributes.value: SAMEA104558087
attributes.tag: INSDC center alias
attributes.value: OREGON STATE UNIVERS
attributes.tag: INSDC center name
attributes.value: Oregon State University
attributes.tag: INSDC first public
attributes.value: 2019-01-10T17:02:35Z
attributes.tag: INSDC last update
attributes.value: 2018-01-31T16:22:53Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6445:Seedling-shoot-2-days-light-replicate-3
attributes.tag: age
attributes.value: 10
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: stiff brome
attributes.tag: developmental stage
attributes.value: LP.02 two leaves visible stage
attributes.tag: ecotype
attributes.value: Bd21
attributes.tag: environmental stress
attributes.value: continuous light regimen
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: growth condition
attributes.value: 48 hours continuous light regimen after 8 days continuous dark (no light) regimen
attributes.tag: organism part
attributes.value: shoot
attributes.tag: sample name
attributes.value: E-MTAB-6445:Seedling-shoot-2-days-light-replicate-3