title: Frog histone reconstitute Mnase
organism: Ovis aries/Saccharomyces cerevisiae mixed DNA library
description: Two plasmid DNAs were employed: pBLG (BLG) comprised 10,841 bp of ovine DNA containing the beta-lactoglobulin gene and 2,020 bp of plasmid vector; p13 (YRO) comprised 13,626 bp of Saccharomyces cerevisiae DNA (chrXIV:243,179-256,806 ((SacCer_Apr2011/sacCer3) assembly)) containing a late-firing replication origin (ARS1413 (~ 250,600-251,220) and 6683 bp of plasmid vector. A mixture of equal weights of linearised plasmids containing BLG and YRO were reconstituted with recombinant Xenopus laevis core histones by salt gradient dialysis. Nucleosomal DNA was prepared from these reconstitutes using micrococcal nuclease. The resulting ~146 bp mononucleosome DNA fragments were purified after electrophoresis.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2012-01-28T17:00:42Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-09T09:55:45Z
attributes.tag: External Id
attributes.value: SAMEA1094822
attributes.tag: INSDC center name
attributes.value: ED
attributes.tag: INSDC first public
attributes.value: 2012-01-28T17:00:42Z
attributes.tag: INSDC last update
attributes.value: 2018-03-09T09:55:45Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: Frog histone reconstitute MNase
attributes.tag: sample name
attributes.value: Frog histone reconstitute MNase
attributes.tag: scientific_name
attributes.value: Ovis aries/Saccharomyces cerevisiae mixed DNA library