title: 16S rDNA amplicon sequencing
organism: groundwater metagenome
description: No industrial activity and no specific geographical location can be disclosed for the four sites studied due to confidentiality reasons. At each site (B, F, G and H), groundwater samples were collected by SITA Remediation Company (Lyon, France) from the monitoring wells drilled in the deep aquifer i.e. between -20 and -30 m depth for the site B, -7 and -12 m for the site F, -7 and -10 m for the site G and -3 and -6 m for the site H.  Genomic DNA (gDNA) was extracted from all groundwater samples according to the experimental protocol described by Dugat-Bony et al. (2011).