title: Eth_Rep1
organism: Saccharomyces cerevisiae
description: Protocols: 10OD of cells were collected, pelleted and snap frozen in liquid nitrogen. The S. cerevisiae strain CEN.PK113-7D was pre-cultured in a batch culture in Delft media with 2% glucose at 30°C and 200 rpm in shake flasks and then transferred to the chemostats in duplicates. Four different media compositions with a limitation in a different nutrient were employed in the chemostat runs. The temperature was also set to 30°C. The dilution rate was fixed to 0.1 and the cells were grown for 4 days to achieve stable cell numbers in the culture. RNA was extracted using the RNeasy® Mini Kit from QIAGEN. Cells were mechanically disrupted using a FastPrep®-24 from MPbio in combination with the lysing matrix tubes type C from MPbio. The FastPrep was run 3 times for 20 seconds with 4.0m/s settings and a 5 minute break in between each run. RNA quality was assessed using NanoDrop and Bioanalyzer. The non-amplification non-tagging CAGE protocol for Illumina sequencing (nAnT-iCAGE) as previously published by Murata et. al. 2014 was used on two biological replicates of each condition, starting with 5 micrograms of extracted RNA.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-01-03T17:03:19Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-04-04T15:15:40Z
attributes.tag: External Id
attributes.value: SAMEA1054242
attributes.tag: INSDC center name
attributes.value: Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, SE-41296, Sweden
attributes.tag: INSDC first public
attributes.value: 2019-01-03T17:03:19Z
attributes.tag: INSDC last update
attributes.value: 2018-04-04T15:15:40Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6650:Eth_Rep1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: baker's yeast
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: growth condition
attributes.value: Delft media with 2 percent glucose
attributes.tag: sample name
attributes.value: E-MTAB-6650:Eth_Rep1
attributes.tag: scientific_name
attributes.value: Saccharomyces cerevisiae
attributes.tag: strain
attributes.value: CEN.PK113-7D