title: Sample 20
organism: Homo sapiens
description: Protocols: Embryonic and fetal bladders and genital tissue were obtained by surgeons from terminated pregnancies after informed consent and with ethics approval. Samples were taken from weeks 7 (n=2), 7,5 (n=1), 8 (n=3), 9 (n=4) for the bladder tissues and from weeks 7 (n=3), 8 (n=3), 9 (n=3) as well as 10 (n=4) for the genital tissues, respectively. Fresh tissues were dissected and immediately submerged in 5 volumes of RNAlater Stabilization Solution (Cat No. AM7020, Thermoisher Scientific, Waltham, MA), incubated at 4°C overnight and then transferred to -20°C. RNA was extracted by using TissueLyser LT (Cat No. 85600, QIAGEN, Hilden, Germany) and RNeasy Fibrous Tissue Mini Kit (cat. no. 74704, QIAGEN, Hilden, Germany). The RNA was measured both quantitatively and qualitatively using NanoDrop (Thermofisher Scientific, Waltham, MA) and Bioanalyzer 2100 (Agilent, Santa Clara, CA) instruments. Paired-end libraries of 2x126 bp with poly-A selection according to Illumina TruSeq Stranded mRNA protocol.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-01-01T17:01:36Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-09T16:53:47Z
attributes.tag: External Id
attributes.value: SAMEA104678760
attributes.tag: INSDC center name
attributes.value: Karolinska Institutet, Department of Women's and Children's Health, Center for Molecular Medicine
attributes.tag: INSDC first public
attributes.value: 2019-01-01T17:01:36Z
attributes.tag: INSDC last update
attributes.value: 2018-03-09T16:53:47Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6592:Sample 20
attributes.tag: age
attributes.value: 10
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: late embryo
attributes.tag: disease
attributes.value: normal
attributes.tag: organism part
attributes.value: reproductive organ
attributes.tag: sample name
attributes.value: E-MTAB-6592:Sample 20
attributes.tag: scientific_name
attributes.value: Homo sapiens