title: Ana_Rep1
organism: Saccharomyces cerevisiae
description: Protocols: 10OD of cells were collected, pelleted and snap frozen in liquid nitrogen. The S. cerevisiae strain CEN.PK113-7D was pre-cultured in a batch culture in Delft media with 2% glucose at 30°C and 200 rpm in shake flasks and then transferred to the chemostats in duplicates. Four different media compositions with a limitation in a different nutrient were employed in the chemostat runs. The temperature was also set to 30°C. The dilution rate was fixed to 0.1 and the cells were grown for 4 days to achieve stable cell numbers in the culture. Each environmental stress condition is described as follows: Low Glucose: minimal media with 0.75 % glucose and 1.35 g/l NH4. Anaerobic condition, low Glucose: minimal media with 1 % glucose and 1.35 g/l NH4, continuous gassed with N2. Low Ethanol: minimal media with 0.5 % ethanol and 1.35 g/l NH4. Low Nitrogen: minimal media with 6 % glucose and 0.27 g/l NH4 RNA was extracted using the RNeasy® Mini Kit from QIAGEN. Cells were mechanically disrupted using a FastPrep®-24 from MPbio in combination with the lysing matrix tubes type C from MPbio. The FastPrep was run 3 times for 20 seconds with 4.0m/s settings and a 5 minute break in between each run. RNA quality was assessed using NanoDrop and Bioanalyzer. The Illumina Kit TrueSeq RNA stranded HT kit was used following the manufacturers protocol.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-01-03T17:03:20Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-04-27T14:17:20Z
attributes.tag: External Id
attributes.value: SAMEA4618626
attributes.tag: INSDC center name
attributes.value: Department of Biology and Biological Engineering, Chalmers University of Technology, Gothenburg, SE-41296, Sweden
attributes.tag: INSDC first public
attributes.value: 2019-01-03T17:03:20Z
attributes.tag: INSDC last update
attributes.value: 2018-04-27T14:17:20Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6722:Ana_Rep1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: baker's yeast
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: growth condition
attributes.value: minimal media in chemostat
attributes.tag: sample name
attributes.value: E-MTAB-6722:Ana_Rep1
attributes.tag: scientific_name
attributes.value: Saccharomyces cerevisiae
attributes.tag: strain
attributes.value: CEN.PK113-7D