title: Snyder_GEI-11_GFP_L3_rep2 extraction1_seq1 aliquote 1
organism: Caenorhabditis elegans
attributes.tag: source_name
attributes.value: Snyder_GEI-11_GFP_L3_rep2
attributes.tag: strain
attributes.value: OP179(official name : OP179 genotype : unc-119(ed3) III; wgIs179 [unc-119(+) gei-11::TY1::EGFP::3xFLAG] outcross : 3 mutagen : Bombard tags : GFP::3xFlag description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Tubiginen using Tony Hyman's recombineering pipeline. The resulting plasmid was used for biolistic transformation of an unc-119(ed3) strain. The GEI-11::EGFP fusion protein is expressed in the correct gei-11 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the GEI-11 transcription factor. made_by : R. Waterston )
attributes.tag: developmental stage
attributes.value: L3
attributes.tag: genotype
attributes.value: unc-119(ed3) III; wgIs179 [unc-119(+) gei-11::TY1::EGFP::3xFLAG]
attributes.tag: Sex
attributes.value: hermaphrodite