title: TPI_PTC100_1_CAAT
organism: Homo sapiens
description: Protocols: Standard lab conditions were used to grow the HeLa cells used for these experiments HeLa cells were grown over night in 6 cm plates and transiently transfected with 300 pmol siRNA using Lipofectamine RNAiMAX (Life Technologies). 24 h post transfection the cells were split 1:2 in 10 cm plates and the day after transfected again with 600 pmol siRNA. RNA samples were obtained from XRN1 knockdown HeLa cells transfected with the indicated reporter mRNA expressing plasmids (TPI-WT or -PTCs). Total RNA was extracted using Isol-RNA-Lysis Reagent. 3' fragments were linker ligated, reverse transcribed and specific PCR products were amplified with gene specific primers.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2014-11-11T17:02:10Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T21:34:45Z
attributes.tag: External Id
attributes.value: SAMEA2755483
attributes.tag: INSDC center name
attributes.value: Institute for Genetics University of Cologne
attributes.tag: INSDC first public
attributes.value: 2014-11-11T17:02:10Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T21:34:45Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-2907:TPI_PTC100_1_CAAT
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: HeLa
attributes.tag: common name
attributes.value: human
attributes.tag: fraction
attributes.value: TPI PTC100
attributes.tag: genotype
attributes.value: XRN1 siRNA-mediated knockdown
attributes.tag: sample name
attributes.value: E-MTAB-2907:TPI_PTC100_1_CAAT
attributes.tag: scientific_name
attributes.value: Homo sapiens