title: Metagenomic sequencing of KB-1 microbial consotrium: KB-1/1,2-DCA-MeOH strain KBDCA1
organism: Dehalococcoides mccartyi
description: Metagenomic sequencing of KB-1: a mixed microbial consortium used to enhance bioremediation of chlorinated solvents in subsurface environments. This series of cultures were originally enriched from contaminated sediment from Southern Ontario in 1996. Subsequent transfers in 1998 were amended with trichloroethene and 5x electron equivalents of methanol (MeOH). The culture was transferred and enriched on 1,2-dichloroethane (1,2-DCA) and 5x MeOH in 2003. Formally named KB-1/1,2-DCA-MeOH after its lineage, electron acceptor and donor. This culture continues to be maintained batch style in anaerobic minimal medium in the Edwards Laboratory at the University of Toronto.
attributes.tag: isol_growth_condt
attributes.value: missing
attributes.tag: lat_lon
attributes.value: 43.6587 N 79.3955 W
attributes.tag: env_biome
attributes.value: bioreactor
attributes.tag: rel_to_oxygen
attributes.value: anaerobe
attributes.tag: geo_loc_name
attributes.value: Canada: Toronto
attributes.tag: env_material
attributes.value: water
attributes.tag: ref_biomaterial
attributes.value: primary publication
attributes.tag: env_feature
attributes.value: anaerobic bioreactor
attributes.tag: depth
attributes.value: groundwater
attributes.tag: collection_date
attributes.value: 2013-02
attributes.tag: num_replicons
attributes.value: missing
attributes.tag: strain
attributes.value: KBDCA1
attributes.tag: BioSampleModel
attributes.value: MIGS.ba
attributes.tag: BioSampleModel
attributes.value: MIGS/MIMS/MIMARKS.water