title: 2496sTS.Mouse.Liver.10.5.Male
organism: Mus musculus
description: Protocols: The organs were dissected from 14 developmental stages (starting at e10.5 until P63). Until e12.5 the dissected 'brain' samples correspond to the whole brain, and from e13.5 onwards they correspond to the cerebral hemispheres (without the olfactory bulbs). Early 'hindbrain' samples correspond to the prepontine hindbrain-enriched brain region (until e15.5) and from then onwards only to the cerebellum. RNA was extracted using the RNeasy protocol from QIAGEN. RNeasy Micro columns were used to extract RNA from small (< 5 mg) or fibrous samples and RNeasy Mini columns were used to extract RNA from larger samples. The tissues were homogenized in RLT buffer supplemented with 40 mM dithiothreitol (DTT) or QIAzol. The RNA-seq libraries were created using the TruSeq Stranded mRNA LT Sample Prep Kit (Illumina).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-12-30T17:01:51Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-05-21T17:56:00Z
attributes.tag: External Id
attributes.value: SAMEA4671624
attributes.tag: INSDC center name
attributes.value: The University of Heidelberg
attributes.tag: INSDC first public
attributes.value: 2018-12-30T17:01:51Z
attributes.tag: INSDC last update
attributes.value: 2018-05-21T17:56:00Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6798:2496sTS.Mouse.Liver.10.5.Male
attributes.tag: age
attributes.value: 10.5
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: embryo
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: liver
attributes.tag: sample name
attributes.value: E-MTAB-6798:2496sTS.Mouse.Liver.10.5.Male
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: CD-1