title: FOXK2_rep2
organism: Homo sapiens
description: Protocols: U2OS cells, were grown in DMEM supplemented with 10% foetal bovine serum. ChIP was performed as previously described (Marais et al., 2010) using ~8-10 x 10+7 U2OS-FOXK2-HF cells stably expressing FOXK2 tagged with Flag and His tags (~4Ã-150 mm plates) or stable U2OS-HF cells containing an empty vector encoding the same tags. Marais A, Ji Z, Child ES, Krause E, Mann DJ, Sharrocks AD. 2010. Cell cycle-dependent regulation of the Forkhead transcription factor FOXK2 by CDK-cyclin complexes. J Biol Chem. 285: 35728-35739. Anti-Flag (Sigma-Aldrich, F3165)