title: Meloidogyne javanica protoplast
organism: Meloidogyne javanica
description: 9-HOT oxylipins used in this study were purchased from Cayman Chemical Company, diluted with MES buffer to the final concentration of 10 mM. 500 fresh hatched sterilized J2 were incubated in each vial for a total of 15,000 juveniles for each oxylipin containing the 13-KOD, 9-HOT or 0.01 M MES buffer with ethanol to the final concentration of 10mM, as control for 3 hours at 26 Celsius, in dark condition. Total RNA extraction from fresh hatched juveniles, juveniles exposed to 9-HOT was performed with Trizol, using Total RNA extraction from C.elegans bioprotocol with modification, the steps 10-12 were replaced by using Arcturus PicoPure RNA isolation Kit, Applied Biosystem and stored frozen at -80 Celsius till further process.