title: SAOS+p53EE/RR
organism: Homo sapiens
description: Protocols: SAOS cells were cultured in Dulbecco's modified Eagle's medium (Life Technologies) supplemented with 10 % fetal bovine serum (PAA) and penicillin/streptomycin (Life Technologies) using standard conditions and procedures. Cells were transduced with recombinant adenovirus containing p53 wildtype, p53 R181E mutant, p53 E180R mutant, p53 R181E_E180R double mutant or GFP as previously described (Cam et al., 2006). ChIP was perfomed with a p53-specific antibody. The DNA was purified with the Qiaquick PCR purification Kit (Qiagen).