title: CONT_1
organism: mixed sample
description: Protocols: Solanum lycopersicum cv. Moneymaker tomato plants were grown in greenhouse from March through December under natural light, which provided a photoperiod ranging from 9h to 15 h, temperature from 16 degrees C to 30 degrees C and relative humidity from 55% to 80%. The seedlings were grown in pots with sterile quartz sand (80%) and sterile pumice (20%) for non-inoculated plants or with a mixture of sand (55%), sterile pumice (20%) and Funneliformis mosseae (formerly Glomus mosseae) inoculum (25%) Gerd. and Trappe (BEG 12) purchased from Agrauxine (Bretagne, France, http://www.agrauxine.fr) for inoculated plants. Forty-five plants divided into 3 groups of fifteen plants according to their growth conditions were considered: plants grown with F. mosseae inoculum (MYC) or without inoculum were watered, once a week, with a modified Long-Ashton solution containing a low phosphorus and nitrogen concentration (3.2 uM P, 1mM N). The fertilized control plants (CONT) were grown without inoculum and watered, once a week, with a commercial fertilizer solution (7.6mM P, 13.48mM N). At the end of the experiment, the MYC plant roots were cut and fungal colonization was assessed according to the Trouvelot system using MYCOCALC software. Fruits were harvested from the CONT and MYC plants when they reached the turning stage. RNA was extracted using the pine tree-method, with the addition of 1% PVPP to the extraction buffer. For each growth condition, we used two biological replicates, each one formed by pooled RNAs of three fruits. Ten micrograms of each RNA sample was sent to Fasteris Life Science Co. (Geneva, Switzerland) where the libraries were produced.