title: primary_monocyte_7
organism: Homo sapiens
description: Protocols: Monocytes were separated with the Miltenyi Monocyte Isolation kit II (Miltenyi biotec) and re-suspended in RPMI 1640 medium (supp. 2 mM L-glutamine 10,000 U/mL penicillin 10 mg/mL streptomycin and 10% (v/v) fetal bovine serum (Invitrogen, Paisley, UK)) Experimental samples were treated with 10ng/ml LPS for 4 hours and the controls were left untreated (n = 4 per group). Total RNA was extracted using the Qiagen RNeasy kit and included an on-column DNase treatment Ribosomal RNA (rRNA) was depleted using an early-access version of the Ribo-Zero Gold rRNA Removal Kit (Epicentre). Strand-specific cDNA libraries were prepared using an early-access version of the ScriptSeq v2 library preparation kit (Epicentre)