title: miRNA_seq_rep1
organism: Mus musculus
description: Protocols: NIH3T3 cells were transfected with a CMV-tdTomato plasmid and sorted based on fluorescence intensity to match the conditions for Ago2-RIP assays. Total RNA was then extracted using RNApure reagent. Small RNA libraries were prepared using NEBNext small RNA library prep set for Illumina (NEB) following manufacturer's protocol.