title: 58888_6-Kidney
organism: Rattus norvegicus
description: Protocols: Animals were bred on a 12-hour light-dark cycle. After weaning at 21 days, they were given normal chow (Teklad 7913 - Harlan Teklad NIH-31 irradiated, 18% protein, 6% fat). At 15 weeks of age they were switched to a 4% NaCl diet (Teklad 7913 modified with 4% NaCl) until they were humanely euthanized with CO2 at 18 weeks of age. All tissues were preserved in RNAlater reagent. 'About 500mg of RNAlater-treated tissue was homogenized in 2mL TRIzol (Invitrogen) and was generally extracted with the manfucturer''s instructions, with the exception that, following phase separation, 250uL of 50% isopropanol was added to the aqueous phase for each 1mL of TRIzol used prior to isopropanol precipitation. DNA was digested by RNAse-free DNAse (Roche). RNA quantity was quantified using Nanodrop (Thermo Fisher) and integrity qualified with Bioanalyzer 2011 (Agilent).' 'Libraries were prepared following the LT protocol of Illumina TruSeq RNA Library Kit v2 according to manufacturer''s instructions. Libraries were quantified by KAPA SYBR FAST Library Quantification kit.'