title: Colonized T. aestivum roots (sample 2)
organism: Triticum aestivum
description: Protocols: T. aestivum seeds were surface-sterilized and germinated (in water agar plates) at 30 degrees C for 12 h under darkness. Germinated seedlings were transferred to sterile glass tubes containing 25 mL Hoagland nutrient solution (two seedlings per tube) and cultivated at 26 degrees C under 14 h light/10 h darkness for 24 h. Each tube were then inoculated with 0.25 mL of A. brasilense solution grown until optical density (600 nm) of 1.0. The inoculated seedlings were incubated for 3 days at 26 degrees C under 14 h light/10 h darkness. RNAqueous (Ambion) SOLiD Total RNA-Seq Kit (barcoded by Transcriptome Multiplexing Kit) (Life Technologies)