title: cpb-3_L4_1-III
organism: Caenorhabditis elegans
description: Protocols: C. elegans CPEB homolog mutants and wild-type N2 were metabolically labelled at lysine and arginine amino acids by feeding light (Lys0 and Arg0) labelled bacteria using SILAC protocol, as previously described [Subasic et al. 2015]. Samples were harvested after two generations at different developmental stages (fog-1 and cpb-2 at L3; cpb-3 at L4; and N2 at L3, L4 and YA) in biological triplicates. RNA extraction was done using TRIzol reagent (15596-026; Life Technologies) in accordance with the manufacturer's protocol and further purified by using DNA-free kit (AM1906; Ambion). Random primed cDNA library was generated from each sample by GATC Biotech as per their \InView Transcriptome Explore\ package.