title: U937 AICAr2
organism: Homo sapiens
description: Protocols: RNA-seq transcriptome analysis of AML cell line U937. Cells were treated with AICAr (0.4 mM) for 24 h. Experiment was performed in biological triplicates. Cells were grown in RPMI + 10% FBS + pen/strep for 24 h in either control conditions or the presence of AICAr AICAr was used in concentration 0.2 mM/mL and treatment duration was 24 h. Total RNA was isolated and purified for polyA mRNA Libraries were prepared using NEBNext Ultra RNA Library Prep Kit for Illumina