title: F-36P
organism: Homo sapiens
description: Protocols: Cell lines were cultured using media conditions as follows. MUTZ-3: 60% alpha-MEM (Life Technologies), 20% FBS (PAA), 20% conditioned medium of cell line 5637, and 10 ng/ml hrGM-CSF (Amgen); F-36P, Kasumi-3, and UCSD-AML1: 80% RPMI 1640 (Life Technologies), 20% FBS, and 10 ng/ml GM-CSF; SB1690CB: 60% alpha-MEM, 20% FBS, 20% conditioned medium of cell line 5637; MOLM-1, and U937: 90% RPMI and 10% FBS. All cultures contained 50 U/ml penicillin and 50 μg/ml streptomycin (Sigma). Total sample RNA was extracted with phenol chloroform and reverse transcribed using Superscript II RT (Invitrogen). The cDNA was sheared with the Covaris device (Covaris S2) and further processed according to the TruSeq RNA Sample Preparation v2 Guide (Illumina).