title: liver_sample_1
organism: Homo sapiens
description: Protocols: pre-extracted RNA was sourced commercially from BioChain Ribosomal depletion and RNA library construction was performed using Truseq Stranded Total RNA kit with Ribo-Zero Human/Mouse/Rat Set A (Illumina, Cat. #RS-122-2201).1ug of total RNA used as starting material. The library preparations were performed according to the manufacturer's protocol (Truseq Stranded Total RNA Sample Prep Guide Pages 58-87) with minor modifications to the fragmentation and the number of PCR cycles (from the recommended 15 down to 8 in order to increase library fragment complexity). After the addition of elute, fragment and high mix to the ribosomal RNA-depleted RNA, samples were incubated on ice and followed immediately by first strand synthesis. The DNA fragments were amplified and the cleaned libraries quantified using high sensitivity Bioanalyzer.