title: Cell 84
organism: Mus musculus
description: Protocols: Murine in vitro Th2 differentiation using Naive (CD4+CD62L+) cells in presence of IL4 and plate coated anti-CD3 and anti-CD28 antibody lysis in Fluidigm C1 according to single cell mRNA library preparation manual “Using the C1TM Single-Cell Auto Prep System to Generate mRNA from Single Cells and Libraries for Sequencing” RT using SMARTer chemistry, PCR with Advantage Polymerase and library prep using Nextera XT with 8x12 barcoding, according to manual “Using the C1TM Single-Cell Auto Prep System to Generate mRNA from Single Cells and Libraries for Sequencing”