title: 11/7/12 5
organism: Mus musculus
description: Protocols: All procedures involving mice were conducted in accordance with approved Institutional Animal Care and Use Committee protocols. LGR5+ and differentiated cells were isolated and incubated in 1.11% formaldehyde for 10 minutes at room temperature. Chromatin was sonicated into 200 to 300bp fragments using the BioRuptor (Diagenode). ChIP-Seq libraries were made using the ChIP-Seq Library Prep Reagent Set (NEB). Immunoprecipitation was performed using 2ug H3K27ac antibody (Active Motif) and 2ug of chromatin for two biological replicates for each cell type.