title: B61 Dif
organism: Mus musculus
description: Protocols: All procedures involving mice were conducted in accordance with approved Institutional Animal Care and Use Committee protocols. WGSBS was performed as previously published (Lister, Nature 462: 315-322) with the following changes. Isolated genomic DNA was fragmented into 300bp pieces using the S220 Focused - ultrasonicator (Covaris). Sequencing libraries were generated with 500ng genomic DNA using the NEBNext genomic sequencing kit (NEB) and Illumina adaptors (PE-102-1001. PE-102- 1002). Libraries were bisulfite converted using Imprint DNA Modification Kit (Sigma). Size selection of the libraries to 300-600bp was performed on the Pippin Prep DNA size selection system (Sage Science). Amplification of libraries was performed in 18 rounds of PCR using Pfu Turbo Cx Hotstart DNA Polymerase (Agilent Technologies).