title: HUVEC1
organism: Homo sapiens
description: Protocols: Cultured HUVECs were dissociated using accutase and collected. Cells were pelleted, immediately snap frozen, and stored at -80 oC prior to RNA isolation. RNA was isolated from snap frozen cell pellets using the RNeasy Mirco Kit (74004, Qiagen), according to manufacturer's guidelines. cDNA libraries were prepared using the SMARTer Stranded Total RNA-Seq Kit v2- Pico Input (634412, Takara) by the University of Michigan Advanced Genomics Core according to manufacturer's guidelines.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-12-20T04:16:53Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-07-17T11:43:25Z
attributes.tag: External Id
attributes.value: SAMEA7066508
attributes.tag: INSDC center name
attributes.value: University of Michigan Health System - Internal Medicine - Gastroenterology
attributes.tag: INSDC first public
attributes.value: 2020-12-20T04:16:53Z
attributes.tag: INSDC last update
attributes.value: 2020-07-17T11:43:25Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-9372:HUVEC1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: endothelial cell of umbilical vein
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: late embryonic stage
attributes.tag: disease
attributes.value: normal
attributes.tag: individual
attributes.value: 19
attributes.tag: organism part
attributes.value: umbilical vein
attributes.tag: phenotype
attributes.value: CD31+CD144+
attributes.tag: sample name
attributes.value: E-MTAB-9372:HUVEC1
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: female