title: ips-KS1-A
organism: Homo sapiens
description: Protocols: Skin tissue (4 mm punch) biopsies from healthy male donors (HM1; 31 years, HM2; 34 years) and azoospermia patients diagnosed with Klinefelter syndrome (KS1; 31 years, KS2; 34 years) at the reproductive medicine unit at the Karolinska Hospital Huddinge, were obtained with a written consent and with the approval of the Stockholm Regional Ethics Board (Dnr:2013/1132-32). RNA was extracted using the RNeasy Mini Kit (Qiagen) with on-column DNaseI treatment (Qiagen) according to the manufacturer's protocol from three different passages of f-HM1 (p8-10), f-HM2 (p7, p10-11), f-KS1 (p5, p8-9), f-KS2 (p5, p7-8), f-HF1 (p12-14), ips-HM1 (p13-15), ips-HM2 (p11-13), ips-KS1 (p13-15), ips-KS2 (p11-13), ips-HF1 (p15-17), and HS980 (p23-25). Library preparation and sequencing was performed at the Science for Life Laboratory, Sweden. Briefly, libraries were made using Illumina TruSeq® Stranded mRNA with poly-A selection.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-10-05T04:02:43Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-10-25T16:28:03Z
attributes.tag: External Id
attributes.value: SAMEA5046580
attributes.tag: INSDC center name
attributes.value: Department for Clinical Science, Intervention and Technology, Division of Obstetrics and Gynecology, K57, Karolinska Institutet
attributes.tag: INSDC first public
attributes.value: 2019-10-05T04:02:43Z
attributes.tag: INSDC last update
attributes.value: 2018-10-25T16:28:03Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7340:ips-KS1-A
attributes.tag: age
attributes.value: 31
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: induced pluripotent stem cell
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: Klinefelter's syndrome
attributes.tag: growth condition
attributes.value: passage 13-15
attributes.tag: individual
attributes.value: 4
attributes.tag: karyotype
attributes.value: 46,XXY
attributes.tag: organism part
attributes.value: skin
attributes.tag: progenitor cell type
attributes.value: fibroblast of dermis
attributes.tag: sample name
attributes.value: E-MTAB-7340:ips-KS1-A
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: male