title: PISH_Humics_T96_RepB
organism: seawater metagenome
description: 16S-seq of April (ice-covered) Barrow seawater incubation: terrestrial dissolved organic matter addition at T=96h
attributes.tag: host
attributes.value: not applicable
attributes.tag: isolation_source
attributes.value: arctic seawater
attributes.tag: collection_date
attributes.value: 2011-04-30
attributes.tag: geo_loc_name
attributes.value: USA: 2.5 km northwest of Barrow, Alaska
attributes.tag: lat_lon
attributes.value: 71.34 N 156.69 W
attributes.tag: samp_collect_device
attributes.value: Acid-washed 20 L HDPE Carboy
attributes.tag: samp_mat_process
attributes.value: Whole seawater (8.75 L) amended with extracted terrestrial organic matter (1.25 L) and incubated for 96 h in a 10 L acid-washed polycarbonate carboy. A subsample was then collected from this bioassay and filtered onto 0.2-µm Supor filter and frozen at -80 ¡C until nucleic acid extraction.
attributes.tag: source_material_id
attributes.value: PISH_8B
attributes.tag: BioSampleModel
attributes.value: Metagenome or environmental