title: HL-60 untreated2
organism: Homo sapiens
description: Protocols: Neutrophils from three patients with CD40L deficiency and three healthy controls were obtained from heparinized blood by dextran sedimentation followed by Ficoll-Hypaque centrifugation. After isolation, neutrophils were resuspended in RPMI 1640 medium, counted and cell viability was determined by Trypan blue exclusion and Annexin V-FITC staining. For the experiments with HL-60 cells, The HL-60 cell line was obtained from ATCC (HL-60 ATCC® CCL-240™) Neutrophils from three patients with CD40L deficiency and three healthy controls were obtained by dextran isolation method and cultured in RPMI 1640 medium in the presence or absence of (100U/mL) IFN-gamma for 2 hours. For the experiments with HL-60 cells, HL-60 cell line was cultured in RPMI + 20% FBS for 6 days in the presence or absence of 500 ng/mL sCD40L and/or 1,0 % dimethyl sulfoxide (DMSO) cDNA libraries were obtained using the Illumina CBot station, and HiScanSQ using the Illumina TruSeq RNA Sample Preparation Kit (Illumina Inc., San Diego, USA,) according to each manufacturer´s instructions.
attributes.tag: ENA first public
attributes.value: 2017-03-14
attributes.tag: ENA last update
attributes.value: 2017-01-09
attributes.tag: External Id
attributes.value: SAMEA44462668
attributes.tag: INSDC center alias
attributes.value: University of Lubeck
attributes.tag: INSDC center name
attributes.value: University of Lubeck
attributes.tag: INSDC first public
attributes.value: 2017-03-14T17:01:21Z
attributes.tag: INSDC last update
attributes.value: 2017-01-09T13:18:24Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-5316:HL-60 untreated2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: HL-60
attributes.tag: cell type
attributes.value: neutrophil progenitor cell
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: acute promyelocytic leukemia
attributes.tag: genotype
attributes.value: somatic genotype
attributes.tag: sample name
attributes.value: E-MTAB-5316:HL-60 untreated2