title: HCC1954 siRNF40 3
organism: Homo sapiens
description: Protocols: HCC1954 cells were grown in RPMI GlutaMax medium supplemented with 10% fetal bovine serum, 100 units/ml penicillin and 100 μg/ml streptomycin at 37 C and 5% CO2 siRNA transfections were performed using Lipofectamine RNAiMAX(Invitrogen) according to the manufacturer's instructions RNA was extracted using QIAzol Lysis Reagent (Qiagen) according to the manufacturer's instructions Libraries were prepared using NETflexTM Rapid Illumina Directional Kit (Bioscientific) according to the manufacturer's instructions after verifying the RNA integrity on a agarose gel. Library quality was checked using an Agilent Bioanalyzer 2100.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-11-16T09:25:22Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-06-19T14:20:03Z
attributes.tag: External Id
attributes.value: SAMEA6999487
attributes.tag: INSDC center name
attributes.value: University Mdical Center Gottingen Clinic for General, Visceral and Pediatric Surgery Tumor Epigenetics
attributes.tag: INSDC first public
attributes.value: 2020-11-16T09:25:22Z
attributes.tag: INSDC last update
attributes.value: 2020-06-19T14:20:03Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-9234:HCC1954 siRNF40 3
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: HCC1954
attributes.tag: cell type
attributes.value: epithelial cell
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: breast ductal adenocarcinoma
attributes.tag: organism part
attributes.value: mammary gland
attributes.tag: rna interference
attributes.value: RNF40 siRNA
attributes.tag: sample name
attributes.value: E-MTAB-9234:HCC1954 siRNF40 3
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: female