title: Doxorubicin-BR2_S32
organism: Escherichia coli
description: doxorubicin
attributes.tag: strain
attributes.value: WO153
attributes.tag: isolate
attributes.value: NA
attributes.tag: host
attributes.value: NA
attributes.tag: isolation_source
attributes.value: NA
attributes.tag: collection_date
attributes.value: 2016/2018
attributes.tag: geo_loc_name
attributes.value: NA
attributes.tag: altitude
attributes.value: NA
attributes.tag: biomaterial_provider
attributes.value: NA
attributes.tag: culture_collection
attributes.value: NA
attributes.tag: depth
attributes.value: NA
attributes.tag: env_broad_scale
attributes.value: NA
attributes.tag: genotype
attributes.value: NA
attributes.tag: host_tissue_sampled
attributes.value: NA
attributes.tag: identified_by
attributes.value: NA
attributes.tag: lab_host
attributes.value: NA
attributes.tag: lat_lon
attributes.value: NA
attributes.tag: mating_type
attributes.value: NA
attributes.tag: passage_history
attributes.value: NA
attributes.tag: samp_size
attributes.value: NA
attributes.tag: serotype
attributes.value: NA
attributes.tag: serovar
attributes.value: NA
attributes.tag: specimen_voucher
attributes.value: NA
attributes.tag: temp
attributes.value: NA
attributes.tag: sample_type
attributes.value: cell culture
attributes.tag: id_espinoza-et-al
attributes.value: doxorubicin|dmso-10|-2|2|10.0|32|s32
attributes.tag: run
attributes.value: 10.0
attributes.tag: id_jira
attributes.value: SOLEXASEQ-1137
attributes.tag: id_orourke-et-al
attributes.value: R10_Doxorubicin_BR2
attributes.tag: id_sequencing-number
attributes.value: S32
attributes.tag: id_barcode-number
attributes.value: 32
attributes.tag: biological_replicate
attributes.value: 2
attributes.tag: sample-category
attributes.value: nuisance_compound
attributes.tag: sample-category_encoding
attributes.value: -2
attributes.tag: cpd_local
attributes.value: doxorubicin
attributes.tag: cpd_universal
attributes.value: doxorubicin
attributes.tag: ctrl_local
attributes.value: dmso-10
attributes.tag: ctrl_universal
attributes.value: dmso
attributes.tag: og_moa
attributes.value: nuisance
attributes.tag: moa
attributes.value: nuisance
attributes.tag: moa_encoding
attributes.value: -2
attributes.tag: moa_color
attributes.value: #d3d3d3
attributes.tag: neu_sub-moa
attributes.value: toxin
attributes.tag: neu_sub-moa_encoding
attributes.value: 3.0
attributes.tag: gene_target_description
attributes.value: For the purposes of this project, I would classify it as a nuisance compound along with CCCP and SDS. We come across doxorubicin fairly frequently as it is made by Streptomyces. It intercalates into DNA – and as a result, inhibits DNA synthesis. DNA intercalators do not make good antibiotics because the target isn't specific to bacteria. Just like, SDS kills bacteria but its mechanism isn't specific to bacteria. We consider all DNA intercalators to be nuisance compounds, but this one is especially frustrating because it is very potent. We can detect activity when it is present at very low levels and it can require a lot of volume of active culture to figure out that the active compound is doxorubicin.
attributes.tag: ORourke_et_al
attributes.value: True
attributes.tag: Espinoza_et_al
attributes.value: False
attributes.tag: BioSampleModel
attributes.value: Microbe, viral or environmental