title: Plant sample from Glycine max
organism: Glycine max
description: For the collection of the plants associated samples to do the bacteria 16S rRNA sequencing, we collected the rhizosphere, endosphere and soil samples of soybean Pioneer 93B15 in both ambient condition and elevated CO2 condition. For collection of rhizosphere, the soil on the surface of soybean root was collected in the phorsphate buffer and we used the vortex to release the soil on the surface of root. And then the root was transferred into a new 50ml tubes for collection of endosphere samples. The bulk soil surrounding the roots were collected and put into the zip lock plastic bags for the soil samples.
attributes.tag: collection_date
attributes.value: 2014-08
attributes.tag: env_biome
attributes.value: soybean agricultural field
attributes.tag: env_feature
attributes.value: Artifical elevated CO2 condition (SoyFACE facility) in Illinois. http://soyface.illinois.edu/
attributes.tag: env_material
attributes.value: Soybean endosphere, rhizosphere and soil surrounding roots
attributes.tag: geo_loc_name
attributes.value: USA: Illinois
attributes.tag: host
attributes.value: Soy
attributes.tag: lat_lon
attributes.value: 40.03 N 88.12 W
attributes.tag: air_temp_regm
attributes.value: 32celsius around in the soybean field in summer in 2014
attributes.tag: altitude
attributes.value: 228 m
attributes.tag: depth
attributes.value: 30cm
attributes.tag: gaseous_environment
attributes.value: We use the SoyFACE facility to increase the CO2 cencentration in the Octagonal plots (20 meters diameters) to a target concentration of 600 ppm in the field.
attributes.tag: host_age
attributes.value: 40 days
attributes.tag: host_genotype
attributes.value: Pioneer 93B15
attributes.tag: host_height
attributes.value: 50cm
attributes.tag: samp_collect_device
attributes.value: SoyFACE facility for treatment of elevated CO2 condition
attributes.tag: samp_size
attributes.value: 100g soil, 20g root and 1g rhizosphere for each sample
attributes.tag: cultivar
attributes.value: Pioneer 93B15
attributes.tag: age
attributes.value: 40 days
attributes.tag: dev_stage
attributes.value: R5
attributes.tag: tissue
attributes.value: Root, rhizosphere, soil
attributes.tag: biomaterial_provider
attributes.value: Schachtman Daniel
attributes.tag: collected_by
attributes.value: Schachtman Daniel
attributes.tag: BioSampleModel
attributes.value: Plant