organism: Arabidopsis thaliana
attributes.tag: age
attributes.value: 1 week
attributes.tag: dev_stage
attributes.value: seedling
attributes.tag: geo_loc_name
attributes.value: USA
attributes.tag: tissue
attributes.value: shoot
attributes.tag: growth_protocol
attributes.value: Seeds were surface sterilized in 12 % (w/v) bleach/0.1 % Tween 20 solution and washed extensively with sterile distilled water. Seeds were vernalized for 3 days at 4 °C in water, sown in two parallel rows on MS/agar plates and covered with a 100 micron nylon membrane. Seedlings were grown on vertical plates in the Conviron PGR15 growth chamber (12:12 h. light:dark, 21 °C, 50 % humidity, and 250 μmol/m2/s light intensity) for 1 week.
attributes.tag: ecotype
attributes.value: Col-0
attributes.tag: BioSampleModel
attributes.value: Plant