title: Nonabokra_D2_T_rep1
organism: Oryza sativa Indica Group
description: Protocols: Full above ground leaf tissue from each biological replicate. All samples collected at 21 days post germination Drought trial took place at the Oregon State University greenhouse with light intensity at 600 micro-mol m -2 s -1 at the base of the plant, relative humidity at 80% and the photoperiod programmed to cycle for 12-hour light and 12-hour dark. Each plant received 1ml of 100x diluted solution of 15-15-15 N-P-K fertilizer and an additional supplement of 10% chelated iron until the first day of stress treatment. Pots were removed from tub of water and allowed to naturally dry out over time to induce drought stress. Total RNA from frozen seedling shoot sample was extracted as per manufacturer's protocol using RNA Plant reagent (Invitrogen Inc., USA), RNeasy kits (Qiagen Inc., USA), and treated with RNase-free DNase (Life Technologies Inc., USA). True-Seq kit (Illumina Inc.) was used to prepare RNA-seq libraries, according to the manufacture's protocol
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-10-05T04:02:43Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-10-22T12:03:42Z
attributes.tag: External Id
attributes.value: SAMEA5040050
attributes.tag: INSDC center name
attributes.value: Botany and Plant Pathology Department Oregon State University
attributes.tag: INSDC first public
attributes.value: 2019-10-05T04:02:43Z
attributes.tag: INSDC last update
attributes.value: 2018-10-22T12:03:42Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7317:Nonabokra_D2_T_rep1
attributes.tag: age
attributes.value: 21
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: long-grained rice
attributes.tag: cultivar
attributes.value: Nonabokra
attributes.tag: developmental stage
attributes.value: seedling development stage
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: vascular leaf
attributes.tag: sample name
attributes.value: E-MTAB-7317:Nonabokra_D2_T_rep1
attributes.tag: scientific_name
attributes.value: Oryza sativa Indica Group
attributes.tag: stimulus
attributes.value: drought environment