title: 13A
organism: Homo sapiens
description: Protocols: Cell lines were grown either in normoxia (21 percent oxygen) or in hypoxia (0.1 percent oxygen). Cells were cultured in 6-well plates, harvested in 600 uL RLT buffer (RNeasy Mini Kit, Qiagen) and homogenized (QIAshredder, Qiagen). Plasmax cell culture medium which is the subject of this study is produced in house and it is not commercially available. However the precise formulation as well as other relevant information on its production are provided in the manuscript. RNA was extracted using the RNease Mini kit (Qiagen) and processed with on column DNAse digestion (Qiagen). Libraries for cluster generation and DNA sequencing were prepared following an adapted method from Fisher et al. (Genome Biol 12, R1, 2011) using Illumina TruSeq RNA Library Preparation Kit v2. Quality and quantity of the DNA libraries were assessed on an Agilent 2200 Tapestation (D1000 screentape) and Qubit (Thermo Fisher Scientific), respectively.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-01-07T17:03:25Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-11-22T09:52:57Z
attributes.tag: External Id
attributes.value: SAMEA5099603
attributes.tag: INSDC center name
attributes.value: Cancer Research UK Beatson Institute
attributes.tag: INSDC first public
attributes.value: 2019-01-07T17:03:25Z
attributes.tag: INSDC last update
attributes.value: 2018-11-22T09:52:57Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6647:13A
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: BT549
attributes.tag: cell type
attributes.value: mammary epithelial cell
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: ductal carcinoma
attributes.tag: growth condition
attributes.value: DMEM-F12 media
attributes.tag: organism part
attributes.value: mammary gland
attributes.tag: sample name
attributes.value: E-MTAB-6647:13A
attributes.tag: scientific_name
attributes.value: Homo sapiens