title: Sample 6 (ULSAM 1242)
organism: Homo sapiens
description: Protocols: Five samples were selected with acquired uniparental disomy of chromosome 22 and various hematological conditions, either CMML, MDS/MPN or age related clonal hematopoiesis. For samples E4051 and E6523, both tumor (peripheral blood leukocyte) and constitutional (cultured T-cell) DNA was extracted. T-cells were isolated from anticoagulated peripheral blood using CD3 MicroBeads and cultured using a T-cell Activation/Expansion kit (Miltenyi Biotec, Bisley, UK). For samples ULSAM1182, ULSAM1242 and ULSAM1356 DNA was extracted from the tumour only (peripheral blood leukocyte). Samples E4051 and E6523 were prepared for exome sequencing using the Agilent SureSelect kit (Agilent Technologies, Palo Alto, CA, USA) (Human All Exon 50 Mb). Whole exome sequencing of samples ULSAM1182, ULSAM1242 and ULSAM1356 was performed separately in Stockholm, Sweden.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-01-22T17:05:49Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-12-18T10:34:30Z
attributes.tag: External Id
attributes.value: SAMEA5186688
attributes.tag: INSDC center name
attributes.value: University of Southampton
attributes.tag: INSDC first public
attributes.value: 2019-01-22T17:05:49Z
attributes.tag: INSDC last update
attributes.value: 2018-12-18T10:34:30Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7491:Sample 6 (ULSAM 1242)
attributes.tag: age
attributes.value: not available
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: leukocyte
attributes.tag: cohort
attributes.value: ULSAM population cohort
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: cancer
attributes.tag: individual
attributes.value: 4
attributes.tag: organism part
attributes.value: blood
attributes.tag: sample name
attributes.value: E-MTAB-7491:Sample 6 (ULSAM 1242)
attributes.tag: sampling site
attributes.value: neoplasm
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: male