title: 4_2_RRHP-Seq
organism: Mus musculus musculus
description: Protocols: Mice were sacrificed via cervical dislocation, and the whole brains were isolated immediately. The hypothalamic ARC tissues were obtained according to the orientation of 0.4 mm lateral, 1.60 mm posterior to bregma, 7.40 mm below the surface of the dura Genomic DNA of ARC in two groups were extracted using the QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany). The 200 ng high-quality DNA was then digested by restriction endonucleases MspI (NEB) and subjected to 3′-end blunting and single nucleotide (A) addition and adaptor ligation. For RRHP, 5hmC positions at the adapter junctions were modified by T4 phage β-glucosyltransferase (NEB), and non glucosyl-5hmCs were removed by another round of MspI digestion. The 250-500 bp fragments were then selected and treated with bisulfite conversion using Epitect Bisulfite Kit (Qiagen) according to the manufacturer's instructions.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-08-21T04:03:38Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-08-11T12:05:55Z
attributes.tag: External Id
attributes.value: SAMEA7175937
attributes.tag: INSDC center name
attributes.value: Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University
attributes.tag: INSDC first public
attributes.value: 2020-08-21T04:03:38Z
attributes.tag: INSDC last update
attributes.value: 2020-08-11T12:05:55Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-9421:4_2_RRHP-Seq
attributes.tag: age
attributes.value: 4
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: eastern European house mouse
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: hypothalamic arcuate nucleus
attributes.tag: sample name
attributes.value: E-MTAB-9421:4_2_RRHP-Seq
attributes.tag: scientific_name
attributes.value: Mus musculus musculus
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: C57BL/6
attributes.tag: sub_species
attributes.value: musculus