title: Pacific oyster gill microbiota treated with None not translocated
organism: Magallana gigas
description: Pacific oyster (Crassostrea gigas) gill microbiota; collected 05-Jun-2012; treated with None not translocated (Sylt D); samples taken 4 days after deployment
attributes.tag: ENA first public
attributes.value: 2016-01-14
attributes.tag: ENA last update
attributes.value: 2016-10-21
attributes.tag: External Id
attributes.value: SAMEA3255710
attributes.tag: INSDC center alias
attributes.value: ALFRED WEGENER INSTITUTE FOR POLAR AND MARINE RESE
attributes.tag: INSDC center name
attributes.value: ALFRED WEGENER INSTITUTE FOR POLAR AND MARINE RESE
attributes.tag: INSDC first public
attributes.value: 2016-01-14T17:03:08Z
attributes.tag: INSDC last update
attributes.value: 2016-10-21T09:39:12Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: Cg183_Gil_SySyC_Jn5
attributes.tag: collection date
attributes.value: 2012-06-05
attributes.tag: common name
attributes.value: Pacific oyster
attributes.tag: environment (biome)
attributes.value: aquatic marine benthic coastal
attributes.tag: environment (feature)
attributes.value: organic
attributes.tag: environment (material)
attributes.value: organic material
attributes.tag: geographic location (country and/or sea)
attributes.value: North Sea
attributes.tag: geographic location (latitude)
attributes.value: 55.028
attributes.tag: geographic location (longitude)
attributes.value: 8.434
attributes.tag: geographic location (region and locality)
attributes.value: Germany Schleswig Holstein Sylt List Wadden Sea Odewatt
attributes.tag: host body habitat
attributes.value: gills
attributes.tag: host body site
attributes.value: gills
attributes.tag: host common name
attributes.value: Pacific oyster
attributes.tag: host life stage
attributes.value: adult
attributes.tag: host subject id
attributes.value: 183
attributes.tag: host taxid
attributes.value: 29159
attributes.tag: host-associated environmental package
attributes.value: host-associated
attributes.tag: investigation type
attributes.value: mimarks-survey
attributes.tag: multiplex identifiers
attributes.value: f: GTCACTCT; r: GCTTGGAT
attributes.tag: pcr conditions
attributes.value: initial denaturation:98degC_30s; denaturation:98degC_9s;annealing:55degC_1min; elongation:72degC_90s; final elongation:72degC_10min; 30 cycles
attributes.tag: pcr primers
attributes.value: 27f-338r
attributes.tag: perturbation
attributes.value: treated with None not translocated (Sylt D)
attributes.tag: project name
attributes.value: PRJEB8492
attributes.tag: sample collection device or method
attributes.value: scalpel
attributes.tag: sample name
attributes.value: Cg183_Gil_SySyC_Jn5
attributes.tag: sample volume or weight for DNA extraction
attributes.value: 0.1
attributes.tag: sequencing method
attributes.value: Sequencing by synthesis Illumina paired-end
attributes.tag: target gene
attributes.value: 16s rRNA
attributes.tag: target subfragment
attributes.value: V1-V2
attributes.tag: temperature
attributes.value: 14