title: ChIP_Cnot1_control_liver_2
organism: Mus musculus
description: Protocols: Mice were maintained on a 12 hr light / dark cycle in a temperature-controlled (22ºC) barrier facility with free access to water and a normal diet (NCD, CA-1, CLEA Japan, Inc.). Control (Cnot1fl/fl) and Cnot1 LKO mice placed on tamoxifen-containing food at 6 weeks of age for 2 weeks. Lysate from liver was subjected to ChIP assay with Histone H3 (tri methyl K4) (H3K4me3) antibody (ab8580; abcam) using SimpleChIP Enzyatic Chromatin IP Kit (#9003; Cell Signaling Technology) with modification. Briefly, after micrococcal nuclease treatment, cross-linked chromatin was incubated with anti-H3K4me3 antibody at 4ºC for 24 hr. The immune complex was washed, and DNA was eluted and de-cross-linked. Libraries for DNA sequence were prepared from DNA isolated by ChIP assays with a KAPA Hyper Prep Kit (Illumina), according to the manufacturer's protocol.
attributes.tag: ENA first public
attributes.value: 2018-04-26
attributes.tag: ENA last update
attributes.value: 2017-09-22
attributes.tag: External Id
attributes.value: SAMEA104313374
attributes.tag: INSDC center alias
attributes.value: Cell Signal Unit, Okinawa Institute of Science and Technology Graduate University
attributes.tag: INSDC center name
attributes.value: Cell Signal Unit, Okinawa Institute of Science and Technology Graduate University
attributes.tag: INSDC first public
attributes.value: 2018-04-26T17:02:33Z
attributes.tag: INSDC last update
attributes.value: 2017-09-22T10:52:20Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-5887:ChIP_Cnot1_control_liver_2
attributes.tag: age
attributes.value: 10
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: genotype
attributes.value: Cnot1 flox/flox
attributes.tag: organism part
attributes.value: liver
attributes.tag: phenotype
attributes.value: wild type phenotype
attributes.tag: sample name
attributes.value: E-MTAB-5887:ChIP_Cnot1_control_liver_2
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: C57BL/6J