title: Inoculated (inoculated with bacterial suspension)
organism: Malus domestica
description: Protocols: The flowering trees were kept at 20C day, 18C night with a relative humidity of 80% Ten l of a bacterial suspension (OD600 = 0.1, ~10E8 cfu/ml) of Erwinia amylovora CFBP 1430 were directly applied to the hypanthium with a pipette Flowers were ground in liquid nitrogen using RNAse-free pestles and mortars. Total RNA from flowers was isolated using the innuPREP Plant RNA Kit (Analytik Jena, Germany) according to the manufacturers instructions. Total RNA was treated with DNase I (Thermo Scientific AG, Reinach, BL, Switzerland) From the total RNA samples polyA+ RNA was isolated, treated with tobacco acid pyrophosphatase (TAP), then a RNA oligonucleotide carrying the T7 RNA promotor sequence was ligated to the RNAs. First-strand cDNA synthesis with oligo(dT)-linker primer was performed and PCR amplified. The full-length cDNAs were ultrasound fragmented, end-repaired and purified using the Agencourt AMPure XP kit (Beckman Coulter Genomics). TruSeq sequencing adapters were ligated to the cDNA fragments and PCR-amplified to about 20-30 ng/l
attributes.tag: ENA first public
attributes.value: 2016-01-18
attributes.tag: ENA last update
attributes.value: 2015-12-10
attributes.tag: External Id
attributes.value: SAMEA3706997
attributes.tag: INSDC center alias
attributes.value: Environmental Genomics and Systems Biology Research Group Institute of Natural Resource Sciences Zurich University of Applied Sciences (ZHAW) School of Life Sciences and Facility Management
attributes.tag: INSDC center name
attributes.value: Environmental Genomics and Systems Biology Research Group Institute of Natural Resource Sciences Zurich University of Applied Sciences (ZHAW) School of Life Sciences and Facility Management
attributes.tag: INSDC first public
attributes.value: 2016-01-18T17:03:19Z
attributes.tag: INSDC last update
attributes.value: 2015-12-10T16:37:25Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-41102:Inoculated (inoculated with bacterial suspension)
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: apple
attributes.tag: growth condition
attributes.value: 20C day and 18C night with 80% relative humidity
attributes.tag: infect
attributes.value: Erwinia amylovora CFBP 1430
attributes.tag: organism part
attributes.value: flower
attributes.tag: sample name
attributes.value: E-MTAB-41102:Inoculated (inoculated with bacterial suspension)
attributes.tag: component_organism
attributes.value: Malus domestica
attributes.tag: component_organism
attributes.value: Malus domestica