title: yL122-gDNA-3
organism: Saccharomyces cerevisiae
description: gDNA sequencing for RM11 pool, replicate 3. The read sequences are structured like HNHNHN-{primer sequence}- {guide sequence} - {structural guide sequence}-{donor sequence}. The first HNHNHN portion are randomized nucleotides introduced for sequencing purposes. The guide sequences is flanked by the primer sequence GGGAGCTGCGATTGGCAG and structural guide sequence GTTTAAGAGCTA. 1 mismatch was allowed and both primer and guide sequence were trimmed in R using Biostrings. Only guides that are between 18 to 21 bp are used for subsequent analysis.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-04-25T17:03:04Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-04-25T14:32:30Z
attributes.tag: External Id
attributes.value: SAMEA4609697
attributes.tag: INSDC center name
attributes.value: EMBL
attributes.tag: INSDC first public
attributes.value: 2018-04-25T17:03:04Z
attributes.tag: INSDC last update
attributes.value: 2018-04-25T14:32:30Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: yL122-gDNA-3
attributes.tag: common name
attributes.value: baker's yeast
attributes.tag: sample name
attributes.value: yL122-gDNA-3
attributes.tag: scientific_name
attributes.value: Saccharomyces cerevisiae