title: mouse single cells from E14 ES cell line
organism: Mus musculus
description: Protocols: Wildtype E14 mESC were cultured under standard culture conditions in Knockout DMEM containing 15% Fetal Calf Serum, 1x Penicillin-Streptomycin-Glutamine, 1x NEAA 2-mercaptoethanol and 1000U Leukemia Inhibitory Factor. Trypisnised single mESC suspension is passed through 30micron filter to remove and clumps. Scripts with different method workflows were downloaded from Fluidigm Script Hub. Approx 3000 single mESC are loaded onto 5-10 micron Fluidigm C1 Single-Cell Auto Prep IFC and cell capture was performed as per manufacturers protocol. Two kinds of spike-ins (ERCC-#4456740 and SIRV-E2 #SKU025.03) were mixed (0.5ul 1:500 diluted ERCCs + 0.6ul 1:500 diluted SIRVs) and added to respective Lysis buffer. The subsequent steps are performed as per manufacturers protocol or described on Fluidigm Script hub. The STRT-Seq libraries were made and sequenced as previously described by Islam et al and Zeisel et al.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2017-03-15T17:01:09Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2017-03-15T10:25:11Z
attributes.tag: External Id
attributes.value: SAMEA103911227
attributes.tag: INSDC center name
attributes.value: Wellcome Trust Sanger Institute
attributes.tag: INSDC first public
attributes.value: 2017-03-15T17:01:09Z
attributes.tag: INSDC last update
attributes.value: 2017-03-15T10:25:11Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-5482:mouse single cells from E14 ES cell line
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: ES-E14
attributes.tag: cell type
attributes.value: mouse embryonic stem cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: growth condition
attributes.value: Serum + LIF condition
attributes.tag: sample name
attributes.value: E-MTAB-5482:mouse single cells from E14 ES cell line
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: strain
attributes.value: 129/Ola