title: hOrg121d.B9B
organism: Homo sapiens
description: Protocols: Organoids were grown according to the Lancaster et al. (Nature Protocols 2014, PMID: 25188634) Single cell suspensions from organoids were obtained by Papain based (Miltenyi Biotec, Neural tissue dissociation kit (P)) or Accutase-based dissociation followed by additional filtering and washing steps. Cell suspensions were loaded on Fluidigm C1 microfluidic chips to capture single cells or sorted into 96-well plates using a FACS Aria III (BD) sorter in single cell sorting mode. cDNA was generated on chip using the Fluidigm C1 platform according to the manufacturer's instructions or using the SmartSeq2 protocol (Picelli et al., Nature Protocols 2014) for sorted cells. cDNA quality and quantity was assessed using Agilent's Bioanalyzer DNA High Sensitivity assay or Fragment Analyzer (AATI) HS NGS Fragment assay. Libraries were constructed using Illumina's Nextera XT kit followed by SPRI bead cleanup. Library quality and quantity was assessed using Agilent's Bioanalyzer DNA High Sensitivity assay.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-10-04T04:04:47Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-08-23T12:26:31Z
attributes.tag: External Id
attributes.value: SAMEA5884980
attributes.tag: INSDC center name
attributes.value: Department of Biosystems Science and Engineering, ETH Zurich
attributes.tag: INSDC first public
attributes.value: 2019-10-04T04:04:47Z
attributes.tag: INSDC last update
attributes.value: 2019-08-23T12:26:31Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8234:hOrg121d.B9B
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: 409b2
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: growth condition
attributes.value: cerebral organoid
attributes.tag: inferred cell type
attributes.value: dorsal intermediate progenitor and neuron
attributes.tag: organism part
attributes.value: brain
attributes.tag: sample name
attributes.value: E-MTAB-8234:hOrg121d.B9B
attributes.tag: sampling time point
attributes.value: 121
attributes.tag: scientific_name
attributes.value: Homo sapiens