title: dCoREST_L_RNAi_3
organism: Drosophila melanogaster
description: Protocols: S2 cells were collected after 6 days of knock down by double stranded RNAs targeting dCoREST and its interactors. D. melanogaster S2 cell were maintained in Schneider's medium (Gibco) supplemented with 10% (v/v) fetal calf serum (BiochromAG) and 1% (v/v) Penicillin-Streptomycin (Gibco) under standard conditions (26 °C). S2 cells were treated with double stranded RNAs targeting EGFP (control), dCoREST, dCoREST-L, dLSD1, dL(3)mbt, dLint-1 and dG9a. Total RNA from Drosophila S2 cells was isolated using the peqGOLD Total RNA Kit (S-Line, Peqlab) according to manufacturer's instruction. RNA-seq libraries were prepared using a TruSeq Stranded mRNA Library Prep kit (Illumina).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-01-01T17:02:02Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-12-06T10:18:11Z
attributes.tag: External Id
attributes.value: SAMEA5150263
attributes.tag: INSDC center name
attributes.value: Institut fur Molekularbiologie und Tumorforschung (IMT) Biomedizinisches Forschungszentrum Philipps-Universitat Marburg
attributes.tag: INSDC first public
attributes.value: 2019-01-01T17:02:02Z
attributes.tag: INSDC last update
attributes.value: 2018-12-06T10:18:11Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7440:dCoREST_L_RNAi_3
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: S2
attributes.tag: common name
attributes.value: fruit fly
attributes.tag: developmental stage
attributes.value: late embryo
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: embryo
attributes.tag: sample name
attributes.value: E-MTAB-7440:dCoREST_L_RNAi_3
attributes.tag: scientific_name
attributes.value: Drosophila melanogaster