title: EMOSE_201706010725Z_DAY3_PUMP_SRF_(3m)_SEQ-(100L-on-membrane)_W0.22-3_N010000793
organism: marine metagenome
description: This sample (EMOSE_N010000793) was collected on board the RV Nereis II (Observatoire Oceanologique de Banyuls), using a high volume well pump, in the context of the EMOSE (2017) Inter-Comparison of Marine Plankton Metagenome Analysis Methods. The sampling event (EMOSE_201706010725Z_DAY3_EVENT-PUMP) occurred at position latitudeN=42.4871 and longitudeE=003.170033, on date/time=2017-06-01T07:25Z00, at a depth of 3 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated and prepared back in the lab using protocol [SEQ-(100L-on-membrane)_W0.22-3] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes and eukaryotes. This sample has replicate sample(s): EMOSE_N010000795,EMOSE_N010000797
attributes.tag: Citation
attributes.value: https://doi.pangaea.de/10.1594/PANGAEA.879516
attributes.tag: ENA first public
attributes.value: 2018-04-26
attributes.tag: ENA last update
attributes.value: 2017-08-14
attributes.tag: Event Date/Time End
attributes.value: 2017-06-01T07:39
attributes.tag: External Id
attributes.value: SAMEA104212464
attributes.tag: INSDC center alias
attributes.value: PANGAEA Data Publisher for Earth and Environmental Science
attributes.tag: INSDC center name
attributes.value: PANGAEA Data Publisher for Earth and Environmental Science
attributes.tag: INSDC first public
attributes.value: 2018-04-26T17:19:58Z
attributes.tag: INSDC last update
attributes.value: 2017-08-14T12:45:47Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Latitude End
attributes.value: 42.486
attributes.tag: Longitude End
attributes.value: 3.172
attributes.tag: Salinity Sensor
attributes.value: 37.6
attributes.tag: Submitter Id
attributes.value: EMOSE_N010000793
attributes.tag: depth
attributes.value: 3
attributes.tag: environment (biome)
attributes.value: Westerlies Biome
attributes.tag: environment (feature)
attributes.value: [SRF] surface water layer (ENVO:00010504) within the epipelagic zone (ENVO:00000209) at the top of the photic zone (ENVO:00000209)
attributes.tag: environment (material)
attributes.value: water (ENVO:00002006), including particulate matter (ENVO:01000060) such as planktonic material (ENVO:01000063)
attributes.tag: environmental package
attributes.value: water
attributes.tag: event date/time start
attributes.value: 2017-06-01T07:25
attributes.tag: further details
attributes.value: http://store.pangaea.de/Projects/EMOSE2017/LOGSHEETS/EMOSE_201706010725Z_DAY3_WETLAB_size-fractionated_100L.pdf
attributes.tag: last update date
attributes.value: 2017-08-10Z
attributes.tag: latitude start
attributes.value: 42.487
attributes.tag: longitude start
attributes.value: 3.17
attributes.tag: marine region
attributes.value: [MS] Mediterranean Sea (MRGID:1905)
attributes.tag: project name
attributes.value: EuroMarine Open Science Experiment (EMOSE) 2017 edition: Inter-Comparison of Marine Plankton Metagenome Analysis Methods
attributes.tag: protocol description
attributes.value: In protocole SEQ-(100L-on-membrane)_W0.22-3, the sample material was pre-filtered on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane filter (ref:TSTP14250), and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 100 Litres was filtered and the 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250) was packaged in a sterile 5-mL cryotube, treated with no addition of chemicals, labelled with a barcode identification sticker, flash frozen in liquid nitrogen and stored in a freezer at -80 degree Celsius. Note 1: The filtration lasted 121 minutes, starting at 10:04 and ending at 12:05 local time. Note 2: Latex or nitril gloves were used for this protocol. All containers, filter holders and tubing were washed with 0.1% bleach, rinsed with miliQ water. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The sample was sent for later analysis to the Genoscope, National Sequencing Centre, Paris, France.
attributes.tag: protocol label
attributes.value: SEQ-(100L-on-membrane)_W0.22-3
attributes.tag: sample collection device
attributes.value: high volume well pump
attributes.tag: sample name
attributes.value: EMOSE_N010000793
attributes.tag: sample status
attributes.value: This version can be used to provide data discovery services
attributes.tag: sampling campaign
attributes.value: EMOSE2017
attributes.tag: sampling platform
attributes.value: RV Nereis II (Observatoire Oceanologique de Banyuls)
attributes.tag: sampling station
attributes.value: DAY3
attributes.tag: size fraction lower threshold
attributes.value: 0.22
attributes.tag: size fraction upper threshold
attributes.value: 3
attributes.tag: temperature
attributes.value: 19