title: Sample 5
organism: Homo sapiens
description: Protocols: HepG2 with different treatment (sodium oleate, E2 and ER-alpha siRNA) were harvested for RNA-seq HepG2 cells were treated with 60 ug/ml sodium oleate, 50 ng/ml E2 and ER-alpha siRNA Total RNA was isolated from the RNA later preserved spleen samples with miRNeasy Mini kit (QIAGEN, Gemany) in accordance to the manufacturers protocol. RNA was quantified using the NanoPhotometer spectrophotometer (Implen Inc., CA, USA). We then measured RNA concentration using Qubit RNA Assay Kit in Qubit 2.0 Flurometer (Life Technologies, CA, USA), and assessed RNA integrity using the RNA Nano 6000 Assay Kit in Bioanalyzer 2100 (Agilent Technologies, CA, USA). All the samples had a high RNA Integrity Number (RIN) score (>9.4). A total of 5ug of RNA per sample was submitted to the Genefund Co. Ltd (Shanghai, China) for library generation and sequencing.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-10-05T04:02:43Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-10-22T16:39:46Z
attributes.tag: External Id
attributes.value: SAMEA5040554
attributes.tag: INSDC center name
attributes.value: Department of Reproductive Endocrinology, Zhejiang Provincal People's Hospital, Hangzhou Medical College. Hangzhou, Zhejiang, P.R. China
attributes.tag: INSDC first public
attributes.value: 2019-10-05T04:02:43Z
attributes.tag: INSDC last update
attributes.value: 2018-10-22T16:39:46Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7318:Sample 5
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: HepG2
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: hepatocellular carcinoma
attributes.tag: organism part
attributes.value: liver
attributes.tag: sample name
attributes.value: E-MTAB-7318:Sample 5
attributes.tag: scientific_name
attributes.value: Homo sapiens