title: E-MTAB-5901:Cnot1_control_liver_ActD_8h_1
organism: Mus musculus
description: Protocols: Mice were maintained on a 12 hr light / dark cycle in a temperature-controlled (22ºC) barrier facility with free access to water and a normal diet (NCD, CA-1, CLEA Japan, Inc.). For comprehensive mRNA half-life profiling, we injected control (Cnot1 fl/fl) and Cnot1 LKO mice, which were placed on a tamoxifen-containing diet at 6 weeks of age for 2 weeks, intraperitoneally with 2 mg actinomycin D per g body weight for 4 and 8 hr, and then liver samples were collected. Total RNA was isolated from liver using Isogen II (Nippongene), according to the manufacturer's protocol 1 µg of total RNA was used for RNA-seq library preparation with TruSeq Stranded mRNA LT Sample Prep Kit (Illumina), according to the manufacturer's protocol.
attributes.tag: ENA checklist
attributes.value: ERC000011
attributes.tag: INSDC center alias
attributes.value: Cell Signal Unit, Okinawa Institute of Science and Technology Graduate University
attributes.tag: INSDC center name
attributes.value: Cell Signal Unit, Okinawa Institute of Science and Technology Graduate University
attributes.tag: INSDC first public
attributes.value: 2018-04-25T17:02:00Z
attributes.tag: INSDC last update
attributes.value: 2017-09-22T11:13:50Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: SRA accession
attributes.value: ERS1938370
attributes.tag: Sample Name
attributes.value: ERS1938370
attributes.tag: age
attributes.value: 10
attributes.tag: alias
attributes.value: E-MTAB-5901:Cnot1_control_liver_ActD_8h_1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: Cnot1 flox/flox
attributes.tag: organism
attributes.value: Mus musculus
attributes.tag: organism part
attributes.value: liver
attributes.tag: phenotype
attributes.value: wild type phenotype
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: C57BL/6J
attributes.tag: title
attributes.value: Cnot1_control_liver_ActD_8h_1