title: Sample 5
organism: Lasius neglectus
description: Protocols: Adult worker Lasius niger, Lasius neglectus, Lasius flavus, Myrmica scabrinodis and Formica fusca ants and adult queen Lasius flavus ants were collected from individual nests in Cambridge, UK. Total RNA was extracted from 10-20 adult ants (or 5 queens) using Trizol (Invitrogen) following the manufacturer's instructions. RNA was treated with DNase (Promega, RQ1 RNase-free DNase). Ribosomal RNA was depleted using the Ribo-Zero kit (Illumina), and remaining RNA subjected to alkaline hydrolysis, followed by acrilamide gel purification of RNA bands within the size range 35-50 nt for L. niger and 70-85 for the other species. All library amplicons were constructed using a small RNA cloning strategy (Cook et al., 2013, PMID 24260463; Guo et al., 2010, PMID 20703300).
attributes.tag: ENA first public
attributes.value: 2017-10-24
attributes.tag: ENA last update
attributes.value: 2017-05-25
attributes.tag: External Id
attributes.value: SAMEA104098233
attributes.tag: INSDC center alias
attributes.value: Division of Virology, University of Cambridge
attributes.tag: INSDC center name
attributes.value: Division of Virology, University of Cambridge
attributes.tag: INSDC first public
attributes.value: 2017-10-24T17:02:53Z
attributes.tag: INSDC last update
attributes.value: 2017-05-25T09:07:00Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-5781:Sample 5
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: organism part
attributes.value: whole organism
attributes.tag: protocol
attributes.value: RNA-seq
attributes.tag: replicate
attributes.value: 1
attributes.tag: sample name
attributes.value: E-MTAB-5781:Sample 5