title: Sample 1
organism: Drosophila melanogaster
description: Protocols: RNA was prepared from adult fly heads Fly stocks and crosses were maintained on standard media (1% agar, 1.5% sucrose, 3% glucose, 3.5% dried yeast, 1.5% maize, 1% wheat, 1% soya, 3% treacle, 0.5% propionic acid, 0.1% Nipagin) RNA was extracted from 20 heads per sample in five independent samples. Heads were homogenised in TRI Reagent (Sigma) by using a motorised pestle and following the manufacturer's instructions. The guidelines from the Illumina TruSeq Stranded mRNA Sample Preparation Guide (Part 15031047 Rev. E, October 2013) were followed
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-05-20T04:02:47Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-05-10T11:28:05Z
attributes.tag: External Id
attributes.value: SAMEA5602948
attributes.tag: INSDC center name
attributes.value: University of Cambridge, MRC Toxicology Unit
attributes.tag: INSDC first public
attributes.value: 2019-05-20T04:02:47Z
attributes.tag: INSDC last update
attributes.value: 2019-05-10T11:28:05Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7952:Sample 1
attributes.tag: age
attributes.value: 10
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: fruit fly
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: diet
attributes.value: normal food
attributes.tag: genotype
attributes.value: da-GAL4
attributes.tag: organism part
attributes.value: head
attributes.tag: rearing temperature
attributes.value: 29
attributes.tag: sample name
attributes.value: E-MTAB-7952:Sample 1
attributes.tag: scientific_name
attributes.value: Drosophila melanogaster
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: wDah