title: DNA blank extraction
organism: metagenome
description: Blank extraction with ddH2O; this was a single NTC with amplificiation
attributes.tag: ENA first public
attributes.value: 2016-01-14
attributes.tag: ENA last update
attributes.value: 2016-10-21
attributes.tag: External Id
attributes.value: SAMEA3256107
attributes.tag: INSDC center alias
attributes.value: ALFRED WEGENER INSTITUTE FOR POLAR AND MARINE RESE
attributes.tag: INSDC center name
attributes.value: ALFRED WEGENER INSTITUTE FOR POLAR AND MARINE RESE
attributes.tag: INSDC first public
attributes.value: 2016-01-14T17:03:09Z
attributes.tag: INSDC last update
attributes.value: 2016-10-21T09:43:22Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: DNA_extract_NTC_IC2012
attributes.tag: collection date
attributes.value: 2012-06-10
attributes.tag: environment (biome)
attributes.value: water
attributes.tag: environment (feature)
attributes.value: water
attributes.tag: environment (material)
attributes.value: water
attributes.tag: geographic location (country and/or sea)
attributes.value: Germany
attributes.tag: geographic location (latitude)
attributes.value: 55.028
attributes.tag: geographic location (longitude)
attributes.value: 8.434
attributes.tag: investigation type
attributes.value: mimarks-survey
attributes.tag: miscellaneous environmental package
attributes.value: miscellaneous natural or artificial environment
attributes.tag: multiplex identifiers
attributes.value: f: TGTCCAGA; r TGTGGACT
attributes.tag: pcr conditions
attributes.value: initial denaturation:98degC_30s; denaturation:98degC_9s;annealing:55degC_1min; elongation:72degC_90s; final elongation:72degC_10min; 30 cycles
attributes.tag: pcr primers
attributes.value: 27f-338r
attributes.tag: project name
attributes.value: PRJEB8492
attributes.tag: sample name
attributes.value: DNA_extract_NTC_IC2012
attributes.tag: sequencing method
attributes.value: Sequencing by synthesis Illumina paired-end
attributes.tag: target gene
attributes.value: 16s rRNA
attributes.tag: target subfragment
attributes.value: V1-V2