title: Saline-4
organism: Mus musculus
description: Protocols: Mice were transcardially perfused with 0.9% saline at 4 °C to remove circulating blood, and brains were removed and collected into RNAlater (Thermofisher Scientific Ltd., UK) prior to storage at -20 °C for later analysis. TMAO-treated mice were injected i.p. with 1.8 mg/kg TMAO in 100 microlitres saline vehicle, a dose calculated to approximate human circulating TMAO levels. Saline-treated animals received only 100 microlitres saline. Whole brain total RNA was extracted using a PureLink RNA Mini Kit (Thermofisher Scientific Ltd., UK). TruSeq Stranded mRNA LT Sample Prep Kit as per the manufacturer's instructions.
attributes.tag: ENA first public
attributes.value: 2020-12-19
attributes.tag: ENA last update
attributes.value: 2020-12-09
attributes.tag: External Id
attributes.value: SAMEA7699742
attributes.tag: INSDC center alias
attributes.value: Dept of Bioscience, School of Science and Technology, Nottingham Trent University
attributes.tag: INSDC center name
attributes.value: Dept of Bioscience, School of Science and Technology, Nottingham Trent University
attributes.tag: INSDC first public
attributes.value: 2020-12-19T04:15:09Z
attributes.tag: INSDC last update
attributes.value: 2020-12-09T16:35:48Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-9869:Saline-4
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: brain
attributes.tag: sample name
attributes.value: E-MTAB-9869:Saline-4
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: C57BL/6J