title: MH1-7
organism: Macaca mulatta
description: Protocols: Cubes were dissected from prefrontal cortex from human, chimpanzee, bonobo and macaque on dry ice aiming for cubes with few curvature to obtain reproducible slicing results. Briefly, the thickness of grey matter at all facets of the cube was measured to obtain a mean gray matter thickness. The mean thickness was divided by 10 to obtain the thickness for each of the segments, whereby each of the segments consisted of several slices at 50 um thickness. Sectioning was performed in a cryostat (Microm, Thermo Fisher), with slices being alternately immersed in Trizol (Invitrogen) for bulk RNA isolation or transferred to a dry tube (low binding) for single nucleus isolation on dry ice. Segments 11 and 12 were collected as well but were considered being derived from white matter of the cortex. Samples were then stored at -80°C until further use. RNA isolation for bulk-RNA Seq was performed using the Direct-zol 96 RNA kit (Zymo Research) and was quantified using Agilent's Bioanalyzer RNA 6000 Nano and Pico kit. Libraries were prepared using the NEBNext Ultra Low RNA Library Prep Kit (New England Biolabs). Library quantification was performed using Agilent's Bioanalyzer DNA 1000 chip kit.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-10-04T04:04:47Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-08-08T16:44:31Z
attributes.tag: External Id
attributes.value: SAMEA5858445
attributes.tag: INSDC center name
attributes.value: Department of Biosystems Science and Engineering, ETH Zurich
attributes.tag: INSDC first public
attributes.value: 2019-10-04T04:04:47Z
attributes.tag: INSDC last update
attributes.value: 2019-08-08T16:44:31Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8231:MH1-7
attributes.tag: age
attributes.value: 9
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: Rhesus monkey
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: normal
attributes.tag: individual
attributes.value: MH1
attributes.tag: organism part
attributes.value: prefrontal cortex
attributes.tag: sample name
attributes.value: E-MTAB-8231:MH1-7
attributes.tag: scientific_name
attributes.value: Macaca mulatta
attributes.tag: segment order
attributes.value: 7
attributes.tag: sex
attributes.value: male