title: P7_5
organism: Mus musculus
description: Protocols: Intestinal epithelial cell isolation are based on protocol by Sato and Clevers, 2013. The proximal half of mouse small intestine was isolated and washed with ice-cold PBS until luminal contents were cleared. The intestine were opened longitudinally cut into ~2mm pieces. These fragments were washed 5 times with ice cold PBS. Tissue pieces were incubated with 2 mM EDTA in ice-cold PBS for 30-60 minutes. Intestinal epithelium cells were isolated from up to 10 fractions after pipetting up and down 5 times with PBS. Sato, T., Clevers, H., 2013. Primary mouse small intestinal epithelial cell cultures. Methods Mol. Biol. 945, 319-328. doi:10.1007/978-1-62703-125-7_19 RNA was isolated with the Quick-RNA Micro prep kit (R1050, Zymo research) NEB Next® Ultra™ RNA Library Prep Kit
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-11-16T09:25:14Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-01-21T20:32:32Z
attributes.tag: External Id
attributes.value: SAMEA6495634
attributes.tag: INSDC center name
attributes.value: Dept. of Clinical and Molecular Medicine Norwegian University of Science and Technology (NTNU)
attributes.tag: INSDC first public
attributes.value: 2020-11-16T09:25:14Z
attributes.tag: INSDC last update
attributes.value: 2020-01-21T20:32:32Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8713:P7_5
attributes.tag: age
attributes.value: 7
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: juvenile
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: individual
attributes.value: E
attributes.tag: organism part
attributes.value: epithelium of small intestine
attributes.tag: sample name
attributes.value: E-MTAB-8713:P7_5
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: strain
attributes.value: C57BL/6