title: Seawater sample 3
organism: seawater metagenome
description: seawater sample taken near the oyster reef at low tide; nutrients, pH and salinity measured at a nearby site, around 300 m away on the same day
attributes.tag: ENA first public
attributes.value: 2016-01-14
attributes.tag: ENA last update
attributes.value: 2016-10-21
attributes.tag: External Id
attributes.value: SAMEA3255995
attributes.tag: INSDC center alias
attributes.value: ALFRED WEGENER INSTITUTE FOR POLAR AND MARINE RESE
attributes.tag: INSDC center name
attributes.value: ALFRED WEGENER INSTITUTE FOR POLAR AND MARINE RESE
attributes.tag: INSDC first public
attributes.value: 2016-01-14T17:03:08Z
attributes.tag: INSDC last update
attributes.value: 2016-10-21T09:44:01Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: SW_Jn6
attributes.tag: ammonium
attributes.value: 0.267
attributes.tag: chlorophyll
attributes.value: 5.4
attributes.tag: collection date
attributes.value: 2012-06-06
attributes.tag: dissolved organic nitrogen
attributes.value: 17.9587
attributes.tag: environment (biome)
attributes.value: aquatic, marine, benthic, coastal
attributes.tag: environment (feature)
attributes.value: oyster reef
attributes.tag: environment (material)
attributes.value: environmental material, water, seawater
attributes.tag: geographic location (country and/or sea)
attributes.value: North Sea
attributes.tag: geographic location (depth)
attributes.value: 0.5
attributes.tag: geographic location (latitude)
attributes.value: 55.028
attributes.tag: geographic location (longitude)
attributes.value: 8.434
attributes.tag: investigation type
attributes.value: mimarks-survey
attributes.tag: multiplex identifiers
attributes.value: f: CAGTTGAC; r TGTGGACT
attributes.tag: nitrate
attributes.value: 0.189
attributes.tag: nitrite
attributes.value: 0.007
attributes.tag: nitrogen
attributes.value: 0.007
attributes.tag: organic nitrogen
attributes.value: 17.9587
attributes.tag: pcr conditions
attributes.value: initial denaturation:98degC_30s; denaturation:98degC_9s;annealing:55degC_1min; elongation:72degC_90s; final elongation:72degC_10min; 30 cycles
attributes.tag: pcr primers
attributes.value: 27F: TCAGAGTTTGATCMTGGCTCAG + 338r: CATGCTGCCTCCCGTAGGAGT
attributes.tag: ph
attributes.value: 8.4
attributes.tag: phosphate
attributes.value: 0.06
attributes.tag: project name
attributes.value: PRJEB8492
attributes.tag: salinity
attributes.value: 30.02241922
attributes.tag: sample collection device or method
attributes.value: tube
attributes.tag: sample material processing
attributes.value: filtering onto 0.2 um filter
attributes.tag: sample name
attributes.value: SW_Jn6
attributes.tag: sample volume or weight for DNA extraction
attributes.value: 100
attributes.tag: sequencing method
attributes.value: Sequencing by synthesis, Illumina paired-end
attributes.tag: silicate
attributes.value: 2.48
attributes.tag: suspended particulate matter
attributes.value: 19.39
attributes.tag: target gene
attributes.value: 16s rRNA
attributes.tag: target subfragment
attributes.value: V1-V2
attributes.tag: temperature
attributes.value: 13.9
attributes.tag: tidal stage
attributes.value: low
attributes.tag: total dissolved nitrogen
attributes.value: 18.47
attributes.tag: water environmental package
attributes.value: water