title: Dongjin-20DAH_seeds
organism: Oryza sativa Japonica Group
description: Protocols: Developing rice (Oryza sativa subsp. japonica) seeds, Dongjin and Iksan 526, respectively, were harvested from one panicle per plant (total four plants per replication) at 6, 13, 20, 31 and 41 days after heading (DAH). Seedlings of wild-type rice (Oryza sativa subsp. japonica cv. Dongjin) and an inbred line of its transgenic counterpart, Iksan 526 (I.526), were used in this study. The seedlings were transplanted in a genetically modified organism (GMO) paddy field (30 cm × 15 cm) of the National Institute of Crop Science (NICS), Republic of Korea, using the standard rice cultivation method of the NICS. A completely randomized design (CRD) was used, with three replications. Total RNA was extracted from Dongjin and Iksan 526 seeds at 20 days after heading using the RNeasy Plant Mini Kit (QIAGEN, Hilden, Germany), according to the manufacturer's instructions. TruSeq RNA Sample Prep Kit v2 was used for library construction.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-12-20T04:17:02Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-10-27T16:16:22Z
attributes.tag: External Id
attributes.value: SAMEA7492759
attributes.tag: INSDC center name
attributes.value: Department of Plant Science, Research Institute for Agriculture and Life Sciences, and Plant Genomics and Breeding Institute, Seoul National University
attributes.tag: INSDC first public
attributes.value: 2020-12-20T04:17:02Z
attributes.tag: INSDC last update
attributes.value: 2020-10-27T16:16:22Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-9695:Dongjin-20DAH_seeds
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: Japanese rice
attributes.tag: cultivar
attributes.value: Dongjin
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: whole seed
attributes.tag: sample name
attributes.value: E-MTAB-9695:Dongjin-20DAH_seeds
attributes.tag: scientific_name
attributes.value: Oryza sativa Japonica Group