title: nascentRNA 72h
organism: Mus musculus
description: Protocols: Primary hematopoietic precursor cells from C57/BL6 age 3 to 6 months old female mice were transformed in vitro by an tamoxifen inducible version of Hoxa9 (Hoxa9-ER). Samples were generated in the presence of active Hoxa9 (0h) and in a time series after Hoxa9 was inactivated by tamoxifen withdrawal at 8h, 16h, 24h, 48h, and 72h. nascent RNA isolation, cells were labeled with 4-thio-uridine for 1h before total RNA was extracted by standard methods. Labeled RNA was specifically biotinylated and separated by streptavidin chromatography. Selected RNA was recovered by cleavage of biotion crosslinkers and subjected to standard library construction methods. Illumina standard RNA library construction kit using Illumina TrueSeq RNA Library Preparation Kit
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-05-20T17:03:34Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-08-06T10:16:20Z
attributes.tag: External Id
attributes.value: SAMEA4819562
attributes.tag: INSDC center name
attributes.value: FAU Erlangen-Nurnberg Dept. Genetics
attributes.tag: INSDC first public
attributes.value: 2019-05-20T17:03:34Z
attributes.tag: INSDC last update
attributes.value: 2018-08-06T10:16:20Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7107:nascentRNA 72h
attributes.tag: age
attributes.value: 3 to 6
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: hematopoietic multipotent progenitor cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: genotype
attributes.value: Hoxa9-ER
attributes.tag: organism part
attributes.value: bone marrow
attributes.tag: phenotype
attributes.value: tamoxifen-inducible overexpression of Hoxa9
attributes.tag: sample name
attributes.value: E-MTAB-7107:nascentRNA 72h
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: C57BL/6