title: Sample 32
organism: Arabidopsis thaliana
description: Protocols: Tissues were collected at the desired growth stage, pooled, snap-frozen in liquid nitrogen and homogenized to a fine powder. Arabidopsis thaliana wild type (Columbia-0) plants were grown on soil or MS media respectively. Total RNA was isolated using the NucleoSpin RNA Plant kit (Macherey-Nagel, Düren, Germany). DNA was remove by on-column treatment with rDNAse (Macherey-Nagel, Düren, Germany). For recalcitrant samples (seed, silique, pollen), we adopted a LiCl-based protocol with minor modifications (Onate-Sanchez and Vicente-Carbajosa, 2008). After LiCl precipitation the RNA pellet was dissolved in rDNAse buffer (Macherey-Nagel, Düren, Germany) and treated with rDNAse (Macherey-Nagel, Düren, Germany). The final pellet was re-suspended in DEPC-treated water. cDNA libraries were prepared using the TruSeq Stranded mRNA Sample Preparation kit (Ilumina, San Diego, CA, USA) according to the manufacturer's instructions.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-01-10T18:10:22Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-05-20T14:08:18Z
attributes.tag: External Id
attributes.value: SAMEA5618915
attributes.tag: INSDC center name
attributes.value: TUM
attributes.tag: INSDC first public
attributes.value: 2020-01-10T18:10:22Z
attributes.tag: INSDC last update
attributes.value: 2019-05-20T14:08:18Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7978:Sample 32
attributes.tag: age
attributes.value: 22
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: thale cress
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: rosette leaf
attributes.tag: sample name
attributes.value: E-MTAB-7978:Sample 32
attributes.tag: sampling site
attributes.value: rosette leaf 1
attributes.tag: scientific_name
attributes.value: Arabidopsis thaliana
attributes.tag: ecotype
attributes.value: Col-0