title: Target capture data for Heliophila elata (S0797), using hyb-seq baits following Nikolov et al. (2019) and Angiosperms-353 (PAFTOL).
organism: Heliophila elata
attributes.tag: isolate
attributes.value: herbarium voucher
attributes.tag: age
attributes.value: dead
attributes.tag: geo_loc_name
attributes.value: South Africa:Along road 364 from Butterkloof Pass to Clanwilliam, 200 m W of Elizabethsfontein junction
attributes.tag: tissue
attributes.value: green leaf
attributes.tag: biomaterial_provider
attributes.value: Klaus Mummenhoff
attributes.tag: collected_by
attributes.value: Mummenhoff, Ramdhani
attributes.tag: collection_date
attributes.value: 2008-09-25
attributes.tag: specimen_voucher
attributes.value: missing
attributes.tag: sample_number_when_collection_private
attributes.value: Klaus Mummenhoff personal collection:Mummenhoff & Ramdhani 65
attributes.tag: DNA_extraction_date
attributes.value: 2020-08-13T00:00:00
attributes.tag: DNA_extraction_done_by
attributes.value: Kasper Hendriks
attributes.tag: DNA_extraction_kit_and_protocol
attributes.value: Qiagen DNeasy PowerPlant Pro Kit (50)
attributes.tag: DNA_extraction_changes_to_protocol
attributes.value: Step 1: added 40 ul of Phenolic Separation Solution as suggested; Step 3, bead beating: 2 x 5 min @ 20 Hz (rotating plates halfway) with Qiagen TissueLyser (of which max speed is 20 Hz); Step 5: used 250 ul of Solution IR as suggested; Step 7: quick spin of 30 s @ 10000G after vortex to get all droplets out of inside cap; Step 13: reloaded flowthrough on to filter membrane for second elution, as suggested.
attributes.tag: DNA_extraction_additional_purification_done
attributes.value: Yes
attributes.tag: DNA_extraction_additional_purification_date
attributes.value: 2020-08-14T00:00:00
attributes.tag: DNA_extraction_additional_purification_done_by
attributes.value: Kasper Hendriks
attributes.tag: Naturalis_Biodiversity_Center_sample_plate
attributes.value: NCBN001243
attributes.tag: Naturalis_Biodiversity_Center_extraction_identifier
attributes.value: 4032059890
attributes.tag: Library_preparation_method
attributes.value: NEBNext Ultra II DNA Library Prep Kit for Illumina (E7645L)
attributes.tag: Library_preparation_start_date
attributes.value: 2020-09-01T00:00:00
attributes.tag: Library_preparation_end_date
attributes.value: 2020-09-01T00:00:00
attributes.tag: Library_preparation_done_by
attributes.value: Kasper Hendriks, Alex Hooft, Elza Duijm
attributes.tag: Target_capture_baits_used
attributes.value: Arbor Bioscience myBaits Expert Angiosperms-353 (item number 308196), mixed with myBaits Custom 20-40K sensu. Nikolov et al. (2019) (item number 300296R); baits from these two kits were mixed prior to hybridization in a 2:1 ratio.
attributes.tag: Hybridization_enrichment_ID
attributes.value: HYB02_ENR07
attributes.tag: Hybridization_temperature_in_degrees_Celsius
attributes.value: 65
attributes.tag: Hybridization_time_in_hours
attributes.value: 24
attributes.tag: Hybridization_cleanup_date
attributes.value: 2020-09-01T00:00:00
attributes.tag: Hybridization_done_by
attributes.value: Kasper Hendriks
attributes.tag: Sequencing_date
attributes.value: 2020-10-13T00:00:00
attributes.tag: Sequencing_company_and_location
attributes.value: BaseClear, Leiden, the Netherlands
attributes.tag: Sequencing_platform
attributes.value: Illumina NovaSeq 6000
attributes.tag: BaseClear_sample_name
attributes.value: S0797_Brassi_HYB02
attributes.tag: BaseClear_reported_number_of_read-pairs
attributes.value: 1483839
attributes.tag: BaseClear_reported_yield_in_mbp
attributes.value: 446
attributes.tag: BaseClear_reported_average_quality
attributes.value: 35.8
attributes.tag: BioSampleModel
attributes.value: Plant