title: Sample 7
organism: Homo sapiens
description: Protocols: RNA was collected using Trizol H1299 cells were transfected with Lipofectamine 2000 with either pIRES puro (empty vector), pIRES puro containing p53-R273H, p53-R273H 22/23, or p53-R273H 22/23 53/54 cDNA. The p53 mutations were created by cloning p53-WT cDNA into the pIRES puro vector and performing site-directed mutagenesis. RNA was extracted using Trizol reagent Libraries were constructed using Illumina TruSeq Stranded Total Ribo-Zero
attributes.tag: ENA first public
attributes.value: 2020-12-18
attributes.tag: ENA last update
attributes.value: 2020-12-16
attributes.tag: External Id
attributes.value: SAMEA7725652
attributes.tag: INSDC center alias
attributes.value: VCU
attributes.tag: INSDC center name
attributes.value: Virginia Commonwealth University
attributes.tag: INSDC first public
attributes.value: 2020-12-18T04:08:22Z
attributes.tag: INSDC last update
attributes.value: 2020-12-16T17:01:45Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-9833:Sample 7
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: H1299
attributes.tag: cell type
attributes.value: lung cancer cell
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: non-small cell lung carcinoma
attributes.tag: genotype
attributes.value: p53-R273H 22/23 53/54 clone 5
attributes.tag: organism part
attributes.value: lung
attributes.tag: sample name
attributes.value: E-MTAB-9833:Sample 7