title: WT+rLCMV-core_day7_r2
organism: Mus musculus
description: Protocols: 5x10^6 naive CD8 T cells from Cor93 TCR transgenic mice (BC10.3) were transferred into C57BL/6 wild-type or MUP-core recipients. Mice were splenectomized and treated with anti-CD62L Abs 48 hours and 4 hours prior to cell transfer, respectively. Wild-type mice were injected with 2.5*10^5 infectious units of non-replicating rLCMV-core 4 hours prior CD8 T cell transfer. Livers were collected and analyzed at day 1, 3 and 7 after CD8 T cell transfer. Naive CD8 T cells were sorted from cor93 TCR transgenic mice (BC10.3). The transposition reaction was performed on 8,000-50,000 cells per replicate, using digitonin 1%, Tn5 transposes and TD Buffer for 45 minutes at 37°C. The reaction was stopped using a solution of 900 mM NaCl and 300 mM EDTA, 5% SDS and Proteinase K for 30 minutes at 40°C. Transposed DNA fragments were purified using AMPure XP beads barcoded with dual indexes and PCR amplified with KAPA HiFi PCR kit.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-01-02T17:01:50Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-12-05T10:30:05Z
attributes.tag: External Id
attributes.value: SAMEA5147928
attributes.tag: INSDC center name
attributes.value: San Raffaele Telethon Institute for Gene Therapy.
attributes.tag: INSDC first public
attributes.value: 2019-01-02T17:01:50Z
attributes.tag: INSDC last update
attributes.value: 2018-12-05T10:30:05Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7461:WT+rLCMV-core_day7_r2
attributes.tag: age
attributes.value: 8 to 10
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: growth condition
attributes.value: transplanted with niave CD8+ ve T cells from Cor93 TCR (BC10.3) transgenic mice
attributes.tag: infect
attributes.value: replication-defective lymphocytic choriomeningitis virus-core
attributes.tag: organism part
attributes.value: liver
attributes.tag: sample name
attributes.value: E-MTAB-7461:WT+rLCMV-core_day7_r2
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: C57BL/6