title: VCaP-CTRL_rep1
organism: Homo sapiens
description: Protocols: For over-expression a derivate of the pLX304 vector was used in which the CMV promoter has been exchanged to a PGK promoter and the blastocidin cassette exchanged by a puromycin resistance cassette (pLX_TRC_307, available at Addgene as Plasmid 41392, pCW107). All ORFs were cloned into pLX_TRC_307-mOrange using Nhe1 and Mlu1. After infection, cells were selected in the presence of puromycin (2 ug/ml). VCaP were grown in DMEM (Gibco) and supplemented with 10% FBS (Gibco), 1% Penicilin/Streptomycin/L-Glutamate. All cells were incubated at 37°C and 5% CO2. Cells were routinely tested for mycoplasma contamination. RNA was extracted using the Rnasy kit (Qiagen) and sample quality was assessed using Agilent Bioanalyzer. RNA libraries were prepared for sequencing using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina®
attributes.tag: ENA first public
attributes.value: 2020-12-17
attributes.tag: ENA last update
attributes.value: 2020-12-16
attributes.tag: External Id
attributes.value: SAMEA7725654
attributes.tag: INSDC center alias
attributes.value: IOR - Institute of Oncology Research
attributes.tag: INSDC center name
attributes.value: IOR - Institute of Oncology Research
attributes.tag: INSDC first public
attributes.value: 2020-12-17T04:08:04Z
attributes.tag: INSDC last update
attributes.value: 2020-12-16T17:23:53Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-71731608139429:VCaP-CTRL_rep1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: VCaP
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: prostate carcinoma
attributes.tag: genetic modification
attributes.value: CTRL
attributes.tag: metastatic site
attributes.value: vertebrae
attributes.tag: organism part
attributes.value: prostate gland
attributes.tag: sample name
attributes.value: E-MTAB-71731608139429:VCaP-CTRL_rep1