title: IO1736_2-20
organism: Mus musculus
description: Protocols: CT-26.WT (5x10^6 cells/mouse) tumor cells were implanted subcutaneously (s.c.) in the left flank of female Balb/c mice. AZD8835 was dosed at 50mg/kg twice daily in at 2days on/ 5 days off schedule for times indicated in figures. Tumor samples were harvested 7 or 14 days after cell implantation respectively. At end of study tumor tissues were then transferred into the gentle MACS C Tube containing RPMI. Tumor samples were processed using the mouse tumor dissociation kit from Miltenyi Biotec. Cells were liberated from tumors for downstream application using a mouse tumor dissociation kit and octodissociator (Miltenyi) according to manufacturer's instructions. For RNA sequencing, total RNA was extracted using the RNeasy 96 Qiacube HT Kit (Qiagen), quality validated using nanodrop and Quantit RNA Assay Kit (Thermo Fisher). TrueSeq Stranded mRNA library preparation
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-01-08T17:03:42Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-11-12T11:43:08Z
attributes.tag: External Id
attributes.value: SAMEA5080123
attributes.tag: INSDC center name
attributes.value: AZ
attributes.tag: INSDC first public
attributes.value: 2019-01-08T17:03:42Z
attributes.tag: INSDC last update
attributes.value: 2018-11-12T11:43:08Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7386:IO1736_2-20
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: CT26.WT
attributes.tag: cell type
attributes.value: fibroblast
attributes.tag: common name
attributes.value: house mouse
attributes.tag: disease
attributes.value: carcinoma
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: growth condition
attributes.value: transplanted until neoplasm formation in BALB/c mice
attributes.tag: organism part
attributes.value: colon
attributes.tag: sample name
attributes.value: E-MTAB-7386:IO1736_2-20
attributes.tag: scientific_name
attributes.value: Mus musculus