title: MIMS Environmental/Metagenome 16S rRNA amplicons from soil microbial community metagenome
organism: soil metagenome
description: The V4 region of 16S rRNA was amplified from pooled DNA samples (4 DNA extracts per amplification) unless otherwise specified. Amplicons were multiplexed for sequencing on Illumina MiSeq: 24 amplicon samples per sequencing run. All together, 6 sequencing runs were performed including a duplicate run, for a total of 22x5=110 soil amplicon samples plus a positive control (E. coli) and a negative control (water) per run.
attributes.tag: collection_date
attributes.value: 2011/2012
attributes.tag: depth
attributes.value: top soil (0-15 cm)
attributes.tag: elev
attributes.value: VT grassland: 1014 ft; VT forest: 1053 ft; NH grassland: 1043 ft; NH forest: 1025 ft
attributes.tag: environment_biome
attributes.value: soil microbial community
attributes.tag: feature
attributes.value: grassland and forest
attributes.tag: geo_loc_name
attributes.value: USA: Vermont; USA, New Hampshire
attributes.tag: lat_lon
attributes.value: VT grassland: 44.1.912 N 72.7.769 W; VT forest: 44.1.84 N 72.7.841 W; NH grassland: 43.34.62 N 71.58.994 W; NH forest: 43.34.51 N 71.58.967 W
attributes.tag: material
attributes.value: soil
attributes.tag: agrochem_addition
attributes.value: Unknown
attributes.tag: annual_season_precpt
attributes.value: see Figure 1 in publication
attributes.tag: annual_season_temp
attributes.value: see Figure 1 in publication
attributes.tag: pool_dna_extracts
attributes.value: 4 DNA extracts were pooled to form one sample unless specified
attributes.tag: samp_collect_device
attributes.value: MoBio Power Soil DNA kit was used to extract DNA from soil.
attributes.tag: samp_mat_process
attributes.value: Four soil samples were extracted per site. Extracted DNA was then pooled to obtain a DNA sample for 16S rRNA amplification.
attributes.tag: samp_size
attributes.value: 250 -500 mg of soil
attributes.tag: soil_type
attributes.value: VT locations: cabot very stoney silt loams; NH locations: skerry fine sandy loams
attributes.tag: soil_type_meth
attributes.value: USDA
attributes.tag: source_mat_id
attributes.value: Location, vegetation, sampling time
attributes.tag: store_cond
attributes.value: frozen at -20C
attributes.tag: BioSampleModel
attributes.value: MIMS.me
attributes.tag: BioSampleModel
attributes.value: MIGS/MIMS/MIMARKS.soil