title: Sample 1
organism: Mus musculus
description: Protocols: Self-maintaining macrophages were sorted on a BD AriaIII (BD Biosciences) in DMEM containing 30% FBS before being pelleted and resuspended in PBS with 0.04% BSA. FACS isolated cells were immediately counted by a Luna-FL Dual Fluorescence Cell Counter (Logos Biosystems). Single cell suspensions were converted to barcoded Drop-seq libraries by using the Chromium Single Cell 3â€™ Library, Gel Bead & Multiplex Kit and Chip Kit (10X Genomics; Pleasanton, California, USA). Samples were processed using kits pertaining to the V2 barcoding chemistry of 10X Genomics.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-07-27T17:01:53Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-07-11T14:46:46Z
attributes.tag: External Id
attributes.value: SAMEA4786373
attributes.tag: INSDC center name
attributes.value: Translational Research Center for Gastrointestinal Disorders (TARGID) Department of Chronic Diseases, Metabolism and Ageing KU Leuven Belgium
attributes.tag: INSDC first public
attributes.value: 2018-07-27T17:01:53Z
attributes.tag: INSDC last update
attributes.value: 2018-07-11T14:46:46Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6976:Sample 1
attributes.tag: age
attributes.value: 35
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: macrophage
attributes.tag: common name
attributes.value: house mouse
attributes.tag: genotype
attributes.value: Cx3cr1CreERT2.Rosa26-LSL-YFP
attributes.tag: organism part
attributes.value: lamina propria of large intestine
attributes.tag: phenotype
attributes.value: YFP+ CD64+
attributes.tag: sample name
attributes.value: E-MTAB-6976:Sample 1
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: female
attributes.tag: stimulus
attributes.value: tamoxifen
attributes.tag: strain
attributes.value: C57BL/6