title: BSA-WGS of Neurospora crassa dim mutants: N7022-LP3 mutant
organism: Neurospora crassa
description: Genomic DNA was isolated using a protocol modified from (Oakley et al, 1987); if Bulk Segregant analysis was to be performed, the genomic DNA from 15 to 50 dim progeny (characterized by a methylation-sensitive restriction enzyme Southern blot) from a cross between the dim mutant (Oakridge) and the SNP mapping strain Mauriceville were equally pooled, and this pool of genomic DNA was subjected to whole genome sequencing. Whole genome sequencing was done with the Nextera kit (Illumina) following manufacturers' protocol, except that after the PCR amplification step, libraries were purified using a Qiagen MinElute kit (PCR purification protocol), fragments between 350 – 550bp were size selected from a 2% TAE gel following gel electrophoresis, and final libraries were purified using a Qiagen MinElute kit (Gel Extraction protocol).
attributes.tag: strain
attributes.value: N2977
attributes.tag: isolate
attributes.value: N7022-LP3
attributes.tag: host
attributes.value: not applicable
attributes.tag: isolation_source
attributes.value: not applicable
attributes.tag: collection_date
attributes.value: 2010/2017
attributes.tag: geo_loc_name
attributes.value: not applicable
attributes.tag: sample_type
attributes.value: whole organism
attributes.tag: biomaterial_provider
attributes.value: Eric U. Selker; N7022-LP3
attributes.tag: collected_by
attributes.value: University of Oregon; Selker lab
attributes.tag: genotype
attributes.value: chap; his-3RIP::barM::his-3RIP, mat a; am132?, inl?; amRIP::hphM::amRIP
attributes.tag: mating_type
attributes.value: a
attributes.tag: passage_history
attributes.value: one to three; asexual culture growth
attributes.tag: BioSampleModel
attributes.value: Microbe, viral or environmental