title: Listeria_Disease_whole blood [MS0108]
organism: Mus musculus
attributes.tag: source_name
attributes.value: Listeria_Disease
attributes.tag: mouse model
attributes.value: Listeria monocytogenes
attributes.tag: condition
attributes.value: Disease
attributes.tag: infection protocol
attributes.value: Listeria monocytogenes was originally obtained from Drs. H. Rogers, K. Murphy, and E. Unanue, DNAX Research Institute, USA. Bacteria were grown in BHI broth to mid-log phase as determined by OD560 and cryopreserved in 20% glycerol/PBS at -80°C. Female C57BL/6 mice were infected by intravenous injection of 5x103 CFU of Listeria monocytogenes derived from cryopreserved stocks diluted in 200 ml of PBS. Control uninfected mice received PBS only. Whole blood samples were collected from individual infected and control treated mice on day 3 post infection.
attributes.tag: tissue
attributes.value: Whole blood