title: Sample 1
organism: Homo sapiens
description: Protocols: Samples from two biological replicates (R1 and R2) were collected. HEK293 cells were grown under standard conditions in DMEM supplemented with 10% (v/v) bovine calf serum (Life Technologies). The cells were treated with small interfering RNAs (siRNAs) targeting PUF60 and RBM39 as described (Kralovicova et al. Nucleic Acids Res, 43:3747, 2015). Total RNA was extracted using RNeasy Plus (Qiagen). The NEBNext poly(A) mRNA magnetic isolation module (E7490L) and the Human/mouse/rat Ribo-Zero™ rRNA Removal Kit (Cambio/Epicentre) for RNA were employed according to manufacturers' recommendations. The libraries were prepared using the NEBNext® Ultra DNA Library Prep Kit for Illumina® (E7370L), size-selected and multiplexed before paired-end sequencing on the HiSeq 2500 Ultra-High-Throughput Sequencing System (Illumina).
attributes.tag: ENA first public
attributes.value: 2018-04-27
attributes.tag: ENA last update
attributes.value: 2017-12-18
attributes.tag: External Id
attributes.value: SAMEA104434036
attributes.tag: INSDC center alias
attributes.value: University of Southampton
attributes.tag: INSDC center name
attributes.value: University of Southampton
attributes.tag: INSDC first public
attributes.value: 2018-04-27T17:02:10Z
attributes.tag: INSDC last update
attributes.value: 2017-12-18T09:40:36Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6010:Sample 1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: 293T
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: late embryonic stage
attributes.tag: organism part
attributes.value: kidney
attributes.tag: replicate
attributes.value: R1
attributes.tag: sample name
attributes.value: E-MTAB-6010:Sample 1