title: E. coli AcrB D408A 1
organism: Escherichia coli str. K-12 substr. MG1655
description: Protocols: Cultures were plunged into 2/5 of ice-cold phenol:ethanol (5% phenol in 95% of pure ethanol). Samples were incubated on ice for 30 minutes and centrifuged at 3500 Xg for 20 minutes at 4C to concentrate the culture. Phenol:ethanol was removed and pellets were stored at -80C overnight before RNA extraction. Protocol from Kroger et al., 2013 doi: 10.1016/j.chom.2013.11.010 Overnight cultures of single colonies were diluted 1:100 in sterile LB broth. Cultures were incubated at 37C, shaking at 200rpm for 10 hours. RNA was extracted from quenched bacterial pellets using RNeasy mini kit (QIAGEN, cat. number 74104). The manufacturer's instructions were followed. Quality of extracted RNA was assessed by Bioanalyzer and Nanodrop. Library was constructed by the Beijing Genomics Institute (BGI) as a contracted service.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-01-13T04:04:18Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-09-20T14:08:21Z
attributes.tag: External Id
attributes.value: SAMEA5789877
attributes.tag: INSDC center name
attributes.value: The University of Birmingham
attributes.tag: INSDC first public
attributes.value: 2020-01-13T04:04:18Z
attributes.tag: INSDC last update
attributes.value: 2019-09-20T14:08:21Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8352:E. coli AcrB D408A 1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: genotype
attributes.value: AcrB D408A
attributes.tag: growth condition
attributes.value: cell suspension culture
attributes.tag: sample name
attributes.value: E-MTAB-8352:E. coli AcrB D408A 1
attributes.tag: scientific_name
attributes.value: Escherichia coli K-12
attributes.tag: strain
attributes.value: K-12
attributes.tag: substrain
attributes.value: MG1655