title: Bps_chronic_Disease_whole blood [MS013]
organism: Mus musculus
attributes.tag: source_name
attributes.value: Bps_chronic_Disease
attributes.tag: mouse model
attributes.value: Burkholderia pseudomallei - chronic
attributes.tag: condition
attributes.value: Disease
attributes.tag: infection protocol
attributes.value: Burkholderia pseudomallei strain 576 originally isolated from a melioidosis patient was provided by Dr. T. Pitt (Health Protection Agency, London, UK). Female C57BL/6 mice were infected intranasally with 50 ml containing 100 colony forming units (chronic model) of B. pseudomallei derived from cryopreserved stocks diluted in pyrogen-free saline. Control uninfected mice received 50 ml pyrogen-free saline only. Whole blood samples were collected from individual infected and control treated mice on days on days 27, 39, 49, 65 and 90 post infection.
attributes.tag: tissue
attributes.value: Whole blood