title: 255
organism: human lung metagenome
description: Spontaneously expectorated sputum from subject 158
attributes.tag: ENA first public
attributes.value: 2018-10-13
attributes.tag: ENA last update
attributes.value: 2017-08-14
attributes.tag: External Id
attributes.value: SAMEA104211888
attributes.tag: INSDC center alias
attributes.value: NATIONAL HEART AND LUNG INSTITUTE, IMPERIAL COLLEGE LONDON
attributes.tag: INSDC center name
attributes.value: NATIONAL HEART AND LUNG INSTITUTE, IMPERIAL COLLEGE LONDON
attributes.tag: INSDC first public
attributes.value: 2018-10-13T17:03:32Z
attributes.tag: INSDC last update
attributes.value: 2017-08-14T10:26:09Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: 255
attributes.tag: adapters
attributes.value: AATGATACGGCGACCACCGAGATCTACAC, CAAGCAGAAGACGGCATACGAGAT
attributes.tag: collection date
attributes.value: 2014-08-14
attributes.tag: common name
attributes.value: human lung metagenome
attributes.tag: environment (biome)
attributes.value: Human
attributes.tag: environment (feature)
attributes.value: Lung
attributes.tag: environment (material)
attributes.value: Sputum
attributes.tag: geographic location (country and/or sea)
attributes.value: United Kingdom
attributes.tag: geographic location (latitude)
attributes.value: 51.487251
attributes.tag: geographic location (longitude)
attributes.value: -0.170825
attributes.tag: host age
attributes.value: 62.28610541
attributes.tag: host disease status
attributes.value: Case
attributes.tag: host subject id
attributes.value: 158
attributes.tag: human associated environmental package
attributes.value: human-associated
attributes.tag: investigation type
attributes.value: metagenome
attributes.tag: library construction method
attributes.value: Cowman et al. 2017
attributes.tag: lung/pulmonary disorder
attributes.value: Secondary bronchiectasis
attributes.tag: multiplex identifiers
attributes.value: CATGCCTAAAGGAGTA
attributes.tag: nebulised_antibiotic_prophylaxis
attributes.value: FALSE
attributes.tag: ntm_species
attributes.value: M. avium complex
attributes.tag: ntm_treatment
attributes.value: TRUE
attributes.tag: nucleic acid amplification
attributes.value: Cowman et al. 2017
attributes.tag: nucleic acid extraction
attributes.value: Cowman et al. 2017
attributes.tag: oral_antibiotic_prophylaxis
attributes.value: FALSE
attributes.tag: oral_corticosteroids
attributes.value: No
attributes.tag: pcr primers
attributes.value: TB11, TB12
attributes.tag: project name
attributes.value: Profiling mycobacterial communities in pulmonary nontuberculous mycobacterial disease
attributes.tag: sample name
attributes.value: 255
attributes.tag: sample volume or weight for DNA extraction
attributes.value: 300ul
attributes.tag: sequencing method
attributes.value: Illumina Miseq
attributes.tag: sputum_aafb_result
attributes.value: No growth
attributes.tag: target gene
attributes.value: hsp65