title: Animal sample from Crassostrea virginica
organism: Crassostrea virginica
description: The American oyster Crassostrea virginica, an ecologically and economically important estuarine organism, can suffer high mortalities in areas in the Northeast United States due to Roseovarius Oyster Disease (ROD), caused by the gram-negative bacterial pathogen Roseovarius crassostreae.  The goals of this research were to provide insights into: 1) the responses of American oysters to R. crassostreae, and 2) potential mechanisms of resistance or susceptibility to ROD.  The responses of oysters to bacterial challenge were characterized by exposing oysters from ROD-resistant (GX3) and susceptible (F3L) families to R. crassostreae, followed by high-throughput sequencing of cDNA samples from various timepoints after disease challenge (1, 5, 15, and 30 days after challenge) .
attributes.tag: geo_loc_name
attributes.value: USA: Delaware Bay, New Jersey
attributes.tag: age
attributes.value: 1 year old
attributes.tag: phenotype
attributes.value: Resistant to challenge with Roseovarius crassostreae
attributes.tag: sex
attributes.value: not determined
attributes.tag: isolation_source
attributes.value: 5 Oysters from nursery cultured in tanks in laboratory for challenge for 4 months
attributes.tag: disease
attributes.value: Roseovarius Oyster Disease (ROD)
attributes.tag: sample_type
attributes.value: Whole organism
attributes.tag: dev_stage
attributes.value: Juveniles (spat or seed)
attributes.tag: breed
attributes.value: GX09
attributes.tag: disease_stage
attributes.value: No signs of ROD
attributes.tag: biomaterial_provider
attributes.value: Ximing Guo, HSRL, Rutgers University
attributes.tag: tissue
attributes.value: Whole oyster
attributes.tag: breeding_method
attributes.value: Family selection
attributes.tag: growth_protocol
attributes.value: Tank culture, recirculation, 28 psu salinity, temperature 18 - 22C
attributes.tag: birth_location
attributes.value: HSRL, Rutgers University
attributes.tag: death_date
attributes.value: 2010
attributes.tag: birth_date
attributes.value: 2009
attributes.tag: store_cond
attributes.value: Whole tissue preserved in RNAlater until isolation
attributes.tag: BioSampleModel
attributes.value: Model organism or animal