title: E+
organism: Achnatherum inebrians
description: Protocols: Both E+ and E- A. inebrians seeds were incubated at 5, 10, 15, 20 and 25 oC in Petri dishes (15-cm diameter). After imbibition, germination rate of seeds was monitored every 24 h during 24-192 h. The seeds showing radical extension of 1 mm were scored and removed from the Petri dishes. After 25 d, when no additional seeds had germinated for 5 d, the germination experiment was terminated. Each treatment was repeated five times independently with 100 seeds in each replicate. Both E+ and E- A. inebrians seeds were sown on filter paper and grown at 10 oC in constant temperature incubators for two days. Total RNAs were isolated from about 10 g of either E+ or E- drunken horse grass seeds using the TRIzol reagent (Invitrogen) and incubated with DNase I (Takara) to remove residual genomic DNA according to manufacturers instruction. The concentration and quantification of all RNA were determined spectrophotometrically.
attributes.tag: ENA first public
attributes.value: 2016-01-20
attributes.tag: ENA last update
attributes.value: 2016-01-10
attributes.tag: External Id
attributes.value: SAMEA3723437
attributes.tag: INSDC center alias
attributes.value: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences
attributes.tag: INSDC center name
attributes.value: Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences
attributes.tag: INSDC first public
attributes.value: 2016-01-20T17:01:29Z
attributes.tag: INSDC last update
attributes.value: 2016-01-10T12:08:18Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-4199:E+
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: infect
attributes.value: N. gansuense
attributes.tag: organism part
attributes.value: seeds
attributes.tag: sample name
attributes.value: E-MTAB-4199:E+