title: MFD1_1
organism: Homo sapiens
description: Protocols: MFD-1 cells were grown in DMEM supplemented with 10% foetal bovine serum and 1% PenStrep. ATAC-seq was performed as published in Buenrostro, J. D., Giresi, P. G., Zaba, L. C., Chang, H. Y., & Greenleaf, W. J. (2013). Transposition of native chromatin for fast and sensitive epigenomic profiling of open chromatin, DNA-binding proteins and nucleosome position. Nature Methods, 10(12), 1213-1218. http://doi.org/10.1038/nmeth.2688 Nuclei were incubated at 37degC for 30 minutes with Tn5 transposase. DNA was purified using Zymo DNA Clean and Concentrator 5. Libraries were constructed as described in Nature Methods, 10(12), 1213-1218.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-04-27T17:02:14Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-04-27T11:47:46Z
attributes.tag: External Id
attributes.value: SAMEA4618214
attributes.tag: INSDC center name
attributes.value: UOM
attributes.tag: INSDC first public
attributes.value: 2018-04-27T17:02:14Z
attributes.tag: INSDC last update
attributes.value: 2018-04-27T11:47:46Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6720:MFD1_1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: MFD-1
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: Oesophageal adenocarcinoma
attributes.tag: organism part
attributes.value: esophagus
attributes.tag: sample name
attributes.value: E-MTAB-6720:MFD1_1
attributes.tag: scientific_name
attributes.value: Homo sapiens