title: Sample 47
organism: Hordeum vulgare subsp. vulgare
description: Protocols: After three weeks of growth, the second expanded leaf after the cotyledon leaf was harvested for two replicate plants per genotype and time point every 4h for 24h starting with lights off. For Bowman, an extra time-point at 14-h was taken. After completion of a 12h dark/12h light diel cycle, the growth chamber was switched to constant light and 20°C and sampling continued for 36 h more every 4 hours starting from the first subjective dusk. Four spring barley genotypes were used, the wild-type spring barley Bowman (BW) and three derived introgression lines with mutations in HvELF3 (BW290), HvLUX1 (BW284) and EARLY MATURITY 7 (EAM7) (BW287). The lines were sown in soil (Einheitserde) in 96-well format. Seeds were maintained at 4°C for three days, followed by germination in 12 h light/12 h dark photoperiods at 20°C with a photon flux density 300µmol m-2 s-1 during the day and 18°C during the night and grown for three weeks. Total RNA was extracted from ground leaf tissue using a hybrid protocol of TRIZOL (Invitrogen) and purification columns from an RNeasy RNA extraction kit (Qiagen). Extracted total RNA was DNAse treated (Ambion). The concentration and integrity of the extracted RNA were determined on a BioAnalyzer (Agilent) before library preparation. The library preparation was carried out following the TruSeq protocol.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-05-25T17:05:27Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-09-30T11:39:36Z
attributes.tag: External Id
attributes.value: SAMEA5983524
attributes.tag: INSDC center name
attributes.value: Institute for Plant Genetics, Heinrich Heine University Duesseldorf
attributes.tag: INSDC first public
attributes.value: 2020-05-25T17:05:27Z
attributes.tag: INSDC last update
attributes.value: 2019-09-30T11:39:36Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8372:Sample 47
attributes.tag: age
attributes.value: 21
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: domesticated barley
attributes.tag: cultivar
attributes.value: BW284
attributes.tag: developmental stage
attributes.value: leaf production stage
attributes.tag: genotype
attributes.value: HvLUX1 mutant
attributes.tag: organism part
attributes.value: leaf
attributes.tag: sample name
attributes.value: E-MTAB-8372:Sample 47
attributes.tag: sampling site
attributes.value: second leaf
attributes.tag: scientific_name
attributes.value: Hordeum vulgare subsp. vulgare
attributes.tag: sub_species
attributes.value: vulgare