title: MG1655 del suhB DNA-seq
organism: Escherichia coli str. K-12 substr. MG1655
description: Protocols: Overnight cultures were started from single colonies in 2 mL LB at 37 C, shaking at 225rpm. Subcultures were set up at 1:100 in 20 mL LB and grown at 37 C, shaking at 225rpm until OD600 reached 0. products were sequenced to identify the presence, if any, of suppressor mutations. Genomic DNA from a strain with wild-type rnc was prepared using a DNeasy Blood and Tissue Kit (Qiagen). Nextera kit (Illumina)
attributes.tag: ENA first public
attributes.value: 2016-01-20
attributes.tag: ENA last update
attributes.value: 2016-01-20
attributes.tag: External Id
attributes.value: SAMEA3730074
attributes.tag: INSDC center alias
attributes.value: Wadsworth Center, NY Department of Health
attributes.tag: INSDC center name
attributes.value: Wadsworth Center, NY Department of Health
attributes.tag: INSDC first public
attributes.value: 2016-01-20T17:01:28Z
attributes.tag: INSDC last update
attributes.value: 2016-01-20T11:46:58Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-4240:MG1655 del suhB DNA-seq
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: genetic modification
attributes.value: deletion suhB
attributes.tag: genotype
attributes.value: thyA suhB::thyA
attributes.tag: sample name
attributes.value: E-MTAB-4240:MG1655 del suhB DNA-seq
attributes.tag: strain
attributes.value: K-12
attributes.tag: substrain
attributes.value: MG1655