title: Sample 25
organism: Populus tremula x Populus tremuloides
description: Protocols: A piece of stem was collected during the harvest and the bark was removed. The woody material was shock frozen in liquid nitrogen and kept at -80 °C. Plants were multiplied by in vitro propagation and kept on 1/2 MS-medium for four weeks. Afterwards, rooted plants were potted in 1.5 l pots and kept in a glass chamber at a temperature of 22 °C, relative humidity of 60% and additional light (16 h, PAR of 100 μmol photons m-2 s-1). The plants were grown there for eight weeks and well irrigated before the treatment started. Well-watered and drought treatments were applied. Control plants were well-watered exhibiting soil moistures around 0.4 m3 m-3. The drought treatment was achieved by stopping irrigation until the soil moisture decreased to 0.1 m3 m-3. Then small amounts of water were applied to each plant to keep soil moisture at this level until the end of the treatment. Nucleic acid was extracted according to CTAB extraction protocol (Chang et al., 1993). TruSeq mRNA Sample Prep kit v2 (Illumina, San Diego, CA, USA) according to manufacturer's instructions.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-01-20T17:02:15Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-01-10T16:27:45Z
attributes.tag: External Id
attributes.value: SAMEA5209702
attributes.tag: INSDC center name
attributes.value: Georg-August-Universitat Busgen-Institut Department of Forest Botany and Tree Physiology
attributes.tag: INSDC first public
attributes.value: 2019-01-20T17:02:15Z
attributes.tag: INSDC last update
attributes.value: 2019-01-10T16:27:45Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7589:Sample 25
attributes.tag: age
attributes.value: 16
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cultivar
attributes.value: T89
attributes.tag: developmental stage
attributes.value: sapling
attributes.tag: environmental stress
attributes.value: drought environment
attributes.tag: genotype
attributes.value: 417-17
attributes.tag: organism part
attributes.value: wood
attributes.tag: sample name
attributes.value: E-MTAB-7589:Sample 25
attributes.tag: scientific_name
attributes.value: Populus tremula x Populus tremuloides