organism: marine metagenome
description: The seasonal structure of the protistan community was examined from March 2011 to July 2013 in a temperate meso-eutrophic coastal system (eastern English Channel; EEC) by means of 18S rRNA tag pyrosequencing. A total of 32 DNA samples were amplified using the two eukaryotic primers 18S-82F 18S-82F (GAAACTGCGAATGGCTC) (Lopez-Garcia et al., 2003) and Euk-516r (ACCAGACTTGCCCTCC) (Amann et al., 1990). These primers have been designed to amplify a domain around 470-480 bp corresponding to the hypervariable V2 and V3 eukaryote 18S rRNA gene regions. The library was prepared following the procedures described by Roche (Basel, Switzerland) and used in one plate run on a 454 GS FLX Titanium Sequencer.
attributes.tag: isolation_source
attributes.value: Sea water
attributes.tag: collection_date
attributes.value: 2011-02/2013-07
attributes.tag: geo_loc_name
attributes.value: France: Eastern English Channel
attributes.tag: lat_lon
attributes.value: 50.679167 N 1.521389 E
attributes.tag: BioSampleModel
attributes.value: Metagenome or environmental