title: GMR/+ Pupa 1
organism: Drosophila melanogaster
description: Protocols: Fly strains were maintained on standard cornmeal food at 18°C. Flies were investigated at the third instar larva stage and in pupa 40 hours after puparium formation. RNA was extracted with mirVana isolation kit according to the manufacturer protocols (mirVana ThermoFisher #AM 1560). Libraries for Illumina sequencing were constructed from 100 ng of total RNA. Libraries for pupal retinae were prepared with random primed cDNA protocol according to GATC biotech company protocols.
attributes.tag: ENA first public
attributes.value: 2018-12-30
attributes.tag: ENA last update
attributes.value: 2017-08-08
attributes.tag: External Id
attributes.value: SAMEA104205812
attributes.tag: INSDC center alias
attributes.value: INGM, National Institute of Molecular Genetics, “Romeo ed Enrica Invernizzi”, Milan, Italy
attributes.tag: INSDC center name
attributes.value: INGM, National Institute of Molecular Genetics, “Romeo ed Enrica Invernizzi”, Milan, Italy
attributes.tag: INSDC first public
attributes.value: 2018-12-30T17:01:43Z
attributes.tag: INSDC last update
attributes.value: 2017-08-08T10:22:25Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-5954:GMR/+ Pupa 1
attributes.tag: age
attributes.value: 40
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: fruit fly
attributes.tag: developmental stage
attributes.value: pupa
attributes.tag: genotype
attributes.value: GMR/+
attributes.tag: organism part
attributes.value: retina
attributes.tag: phenotype
attributes.value: wild type phenotype
attributes.tag: sample name
attributes.value: E-MTAB-5954:GMR/+ Pupa 1