title: Vaccinia virus WR DelB1mutB12-A3_Wiebe
organism: Vaccinia virus WR
description: The B1 gene was replaced with the mCherry gene under the late p11 vaccinia virus promoter. The B1 deletion virus was passaged serially seven times (MOI 0.1) in CV1 cells and later amplified in B1myc expressing CV1 cells for sequencing.
attributes.tag: strain
attributes.value: WR
attributes.tag: collected_by
attributes.value: Annabel Olson
attributes.tag: collection_date
attributes.value: 2016
attributes.tag: geo_loc_name
attributes.value: USA: Nebraska
attributes.tag: host
attributes.value: n/a
attributes.tag: host_disease
attributes.value: n/a
attributes.tag: isolation_source
attributes.value: n/a
attributes.tag: lat_lon
attributes.value: n/a
attributes.tag: passage_history
attributes.value: 7 serial passages at MOI 0.1 in CV1 cells, amplified in B1myc expressing CV1 cells
attributes.tag: BioSampleModel
attributes.value: Pathogen.cl