title: E-MTAB-5448:T0R3
organism: Cellulophaga baltica
attributes.tag: Alias
attributes.value: E-MTAB-5448:T0R3
attributes.tag: Broker name
attributes.value: ArrayExpress
attributes.tag: Description
attributes.value: Protocols: Bacterial isolates C. baltica strains #18 and NN016038 and phages phi38:1 and phi18:3 used in this study were previously isolated and characterized(Holmfeldt, Middelboe et al. 2007, Holmfeldt, Solonenko et al. 2013, Holmfeldt, Howard-Varona et al. 2014, Dang, & Howard-Varona et al. 2015), and the genomes are publicly available: C. baltica #18 (originally at GenBank # CP009976 and refined at https://de.cyverse.org/de/?type=data&folder=/iplant/home/shared/iVirus/ /Cellulophaga_Transcriptomics/Cellulophaga_baltica_strain#18), C. baltica NN016038 (GenBank # CP009887.1), phi18:3 (Genbank # NC_021794.1), phi38:1 (originally at GenBank # NC_021796.1 and refined at https://de.cyverse.org/de/?type=data&folder=/iplant/home/shared/iVirus/ /Cellulophaga_Transcriptomics/). RNA extractions were performed with the RNeasy Mini Kit (Qiagen cat. no. 74104), DNase treated with TURBO DNA-free (Ambion AM1907) and concentrated to 20 μl with the RNA Clean and Concentrator -5 kit (Zymo Research R1015). The RNA integrity number (RIN) and concentration of the extracted RNA was determined via the Agilent Bioanalyzer 2100 (Agilent Technologies, USA).Libraries of complementary DNA were prepared for SOLiD 5500 XL (Applied Biosystems) sequencing according to the manufacturer's protocol (Life Technologies, USA).
attributes.tag: ENA checklist
attributes.value: ERC000011
attributes.tag: INSDC center name
attributes.value: The Ohio State University
attributes.tag: INSDC first public
attributes.value: 2017-03-20T17:01:23Z
attributes.tag: INSDC last update
attributes.value: 2017-02-13T11:27:21Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: SRA accession
attributes.value: ERS1555282
attributes.tag: Sample Name
attributes.value: ERS1555282
attributes.tag: Title
attributes.value: T0R3
attributes.tag: organism
attributes.value: Cellulophaga baltica
attributes.tag: strain
attributes.value: #18