title: RVARBCL 244691
organism: Mus musculus
description: Protocols: Small intestine of mice was isolated, flushed with water. Approximately 7cm from the end of proximal small intestine (stomach end), 0.5cm of tissue was isolated and then transferred to RNA alter and stored at -80 until processing Whole pieces of small intestinal tissue were used for RNA purification using RNeasy Mini Kit (QIAGEN, #74104) according to the manufacturer's instructions. RNA integrity was analysed with a NanoChip (Agilent RNA 6000 Nanokit #5067-1511). A total of 2µg of RNA was purified via Poly-A selection. TruSeq RNA Library Prep Kit v2
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-01-15T17:03:25Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-01-14T12:26:51Z
attributes.tag: External Id
attributes.value: SAMEA5223735
attributes.tag: INSDC center name
attributes.value: CRUK Beatson Institute, Garscube Estate, Switchback Road, Glasgow, G61 1B
attributes.tag: INSDC first public
attributes.value: 2019-01-15T17:03:25Z
attributes.tag: INSDC last update
attributes.value: 2019-01-14T12:26:51Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7546:RVARBCL 244691
attributes.tag: age
attributes.value: 12
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: disease
attributes.value: intestinal cancer
attributes.tag: genotype
attributes.value: VillinCreER Apcfl/fl
attributes.tag: individual
attributes.value: 244691
attributes.tag: organism part
attributes.value: small intestine
attributes.tag: phenotype
attributes.value: epithelium-specific Apc knockout
attributes.tag: sample name
attributes.value: E-MTAB-7546:RVARBCL 244691
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: C57BL/6