title: Sample 13
organism: Populus tremula x Populus tremuloides
description: Protocols: A piece of stem was collected during the harvest and the bark was removed. The woody material was and shock frozen in liquid nitrogen and kept at -80 °C. Plants were multiplied by in vitro propagation and kept on 1/2 MS-medium for four weeks. Afterwards, rooted plants were potted in 1.5 L pots and kept in a CO2 chamber at a temperature of 22 °C, relative humidity of 80% and additional light (16 h, PAR of 100 μmol photons m-2 s-1). Soil mixed with 25 % v/v coarse sand (Ø 0.71 - 1.25 mm) and 25 % v/v fine sand (Ø 0.4 - 0.8 mm) was used. The chamber was supplemented with 1000 ppm CO2. The plants were grown there for six weeks and well irrigated before the treatment started. Three treatments were applied for about 4 weeks: well-watered (control), ABA feeding and drought. The control plants and ABA-fed plants were well-watered with tap water or ABA solution (ABA dissolved in tap water, 100 µM in the first two weeks and 200 µM from the 3rd week on) to keep the soil moisture around 0.4 m3 m-3. The soil moisture of drought-treated plants was gradually decreased from 0.4 to 0.1 m3 m-3 in the first two weeks and was kept constantly between 0.1 and 0.15 m3 m-3 afterwards. Nucleic acid was extracted according to CTAB extraction protocol (Chang et al., 1993). TruSeq mRNA Sample Prep kit v2 (Illumina, San Diego, CA, USA) according to manufacturer's instructions.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-01-20T17:02:15Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-01-10T16:28:24Z
attributes.tag: External Id
attributes.value: SAMEA5209749
attributes.tag: INSDC center name
attributes.value: Georg-August-Universitat Busgen-Institut Department of Forest Botany and Tree Physiology
attributes.tag: INSDC first public
attributes.value: 2019-01-20T17:02:15Z
attributes.tag: INSDC last update
attributes.value: 2019-01-10T16:28:24Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7588:Sample 13
attributes.tag: age
attributes.value: 14
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cultivar
attributes.value: T89
attributes.tag: developmental stage
attributes.value: sapling
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: debarked wood
attributes.tag: phenotype
attributes.value: wild type
attributes.tag: sample name
attributes.value: E-MTAB-7588:Sample 13
attributes.tag: scientific_name
attributes.value: Populus tremula x Populus tremuloides
attributes.tag: stimulus
attributes.value: control