title: MSL2 iCLIP replicate 1
organism: Drosophila melanogaster
description: Protocols: Clone 8 cells, expressing roX1 and roX2, were grown in M3 + 2% FCS + 5 ug/ml insulin + 2.5% fly extract at 25C. We have isolated nuclei, and used nuclear extracts in all experiments. We used Clone8 cells and antibodies against MLE (rat1) and MSL2 (d300, Santa Cruz). One 150mm dish of ~80% confluent cells is used per immunoprecipitation; 6uL from each antibody and 100uL slurry of Protein G (for MLE) or protein A (MSL2) Dynal beads (Invitrogen). The low-RNaseI (Ambion) concentration used for library-preparation was 0.5U/mL. Biological triplicates were tagged with different barcodes and mixed together after reverse-transcription.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2013-07-18T17:00:55Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T16:27:31Z
attributes.tag: External Id
attributes.value: SAMEA2073061
attributes.tag: INSDC center name
attributes.value: University Freiburg
attributes.tag: INSDC first public
attributes.value: 2013-07-18T17:00:55Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T16:27:31Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-1669:MSL2 iCLIP replicate 1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: clone 8
attributes.tag: cell type
attributes.value: wing disc derived
attributes.tag: common name
attributes.value: fruit fly
attributes.tag: developmental stage
attributes.value: 3rd instar larva
attributes.tag: genotype
attributes.value: CME W1 cl.8+
attributes.tag: sample name
attributes.value: E-MTAB-1669:MSL2 iCLIP replicate 1
attributes.tag: scientific_name
attributes.value: Drosophila melanogaster
attributes.tag: sex
attributes.value: male
attributes.tag: specimen with known storage state
attributes.value: frozen specimen