title: Control 1 Day 3
organism: human gut metagenome
description: fecal sample of Control 1 on day 3 of the follow-up
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2013-07-31T17:01:44Z
attributes.tag: ENA-KEYWORD
attributes.value: GSC, MIxS, MIMS
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-09T10:01:26Z
attributes.tag: External Id
attributes.value: SAMEA2164646
attributes.tag: Geographic location (country:region,area)
attributes.value: Spain
attributes.tag: IHMC ethnicity
attributes.value: caucasian
attributes.tag: INSDC center name
attributes.value: CSISP
attributes.tag: INSDC first public
attributes.value: 2013-07-31T17:01:44Z
attributes.tag: INSDC last update
attributes.value: 2018-03-09T10:01:26Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: C1-D3
attributes.tag: age
attributes.value: 66
attributes.tag: body mass index
attributes.value: 31.7
attributes.tag: body product
attributes.value: feces
attributes.tag: broker name
attributes.value: EMG
attributes.tag: collection date
attributes.value: 2010-06-25
attributes.tag: diet
attributes.value: mediterranean diet
attributes.tag: enviornmental package
attributes.value: human-gut
attributes.tag: environment (biome)
attributes.value: terrestrial biome ENVO:00000446
attributes.tag: environment (featurel)
attributes.value: human-associated habitat
attributes.tag: environment (material)
attributes.value: feces
attributes.tag: family relationship
attributes.value: husband of P1
attributes.tag: geographic location (latitude)
attributes.value: 39.46972222222
attributes.tag: geographic location (longitude)
attributes.value: -0.3775
attributes.tag: host subject id
attributes.value: C1
attributes.tag: investigation type
attributes.value: MIxS
attributes.tag: nucleic acid extraction
attributes.value: DNA and RNA were simultaneously extracted from cell pellets using the AllPrep DNA/RNA Mini Kit (QIAGEN)
attributes.tag: project name
attributes.value: Follow-up-IBS
attributes.tag: sample collection device or method
attributes.value: Feces were self-collected by the volunteers in tubes containing 10 mL of phosphate buffered saline (PBS). Samples were kept at 4 ºC until their storage at -80 ºC (approximately 2 hours after being collected)
attributes.tag: sample material processing
attributes.value: Before nucleic acid extraction, fecal samples were resuspended in PBS and centrifuged at 4,000 rpm for 3 min to remove fecal debris. 1-4 mL of the supernatants were centrifuged at 14,000 rpm for 3 min to recover cell pellets
attributes.tag: sample name
attributes.value: C1-D3
attributes.tag: sample storage temperature
attributes.value: -80 ºC
attributes.tag: scientific_name
attributes.value: human gut metagenome
attributes.tag: sequencing method
attributes.value: Pyrosequencing
attributes.tag: sex
attributes.value: male
attributes.tag: smoker
attributes.value: ex-smoker