title: lib02345
organism: Bos taurus
description: Protocols: Multilayers of bovine dorsal soft palate cells were propagated at the air-liquid interface for 5 weeks before infection. At days 0, 1 and 28, cells from 2 inserts were lysed with 750 μl of TRIzol Reagent (Life Technologies, Carlsbad, CA, USA) and frozen at -80 °C. 150 µL of trichlormethane (Carl Roth, Karlsruhe, Germany) was added to lysed cells in 750 μl TRIzol Reagent and the RNA-containing aqueous phase was then mixed with an equal amount of 100% ethanol (Carl Roth). Total RNA was extracted using the RNeasy Mini Kit (Qiagen, Hilden, Germany) with on-column DNase digestion with the RNase-Free DNase Set (Qiagen). Polyadenylated mRNA was isolated from 1-3 µg of high-quality total RNA using the Dynabeads mRNA DIRECT Micro kit (Invitrogen). For preparation of whole-transcriptome libraries the Ion Total RNA-Seq Kit v2 (Life Technologies) was used (1 - 100 ng mRNA, RNase III treatment at 37°C for 10 min). Libraries were quantified using the KAPA Library Quantification Kit Ion Torrent (Kapa Biosystems, Wilmington, MA, USA) on a CFX96 Real-Time PCR Detection System (Bio-Rad Laboratories, München, Germany).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-01-18T17:03:31Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-01-17T10:19:57Z
attributes.tag: External Id
attributes.value: SAMEA5228026
attributes.tag: INSDC center name
attributes.value: Friedrich-Loeffler-Institut Federal Research Institute for Animal Health Institute of Diagnostic Virology
attributes.tag: INSDC first public
attributes.value: 2019-01-18T17:03:31Z
attributes.tag: INSDC last update
attributes.value: 2019-01-17T10:19:57Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7605:lib02345
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: epithelial cell
attributes.tag: common name
attributes.value: cattle
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: wild type
attributes.tag: individual
attributes.value: Animal 2
attributes.tag: infect
attributes.value: foot-and-mouth disease virus
attributes.tag: organism part
attributes.value: soft palate
attributes.tag: sample name
attributes.value: E-MTAB-7605:lib02345
attributes.tag: scientific_name
attributes.value: Bos taurus
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: Swedish Red-and-White