title: WT_expo_2
organism: Dickeya dadantii
description: Protocols: Cells were collected in exponential phase (OD=0.2 at 600nm) or transition to stationary phase (OD=1.5 at 600nm). Growth in minimal medium M63 supplemented with sucrose (0.2% wt/vol) Total RNA from each D. dadantii sample was isolated using the frozen acid phenol method (Maes et Messens 1992), and submitted to Vertis Biotechnologie AG for ribosomal depletion (Illumina RiboZero kit) and RNA fragmentation. Strand-specific cDNA libraries were constructed by Vertis Biotechnologie AG.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-04-16T17:03:56Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-02-01T16:37:08Z
attributes.tag: External Id
attributes.value: SAMEA5315649
attributes.tag: INSDC center name
attributes.value: INSA Lyon
attributes.tag: INSDC first public
attributes.value: 2019-04-16T17:03:56Z
attributes.tag: INSDC last update
attributes.value: 2019-02-01T16:37:08Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7650:WT_expo_2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: growth condition
attributes.value: M63, sucrose
attributes.tag: growth phase
attributes.value: exponential
attributes.tag: sample name
attributes.value: E-MTAB-7650:WT_expo_2
attributes.tag: scientific_name
attributes.value: Dickeya dadantii
attributes.tag: strain
attributes.value: 3937