title: ambystoma mexicanum tissue pool
organism: Ambystoma mexicanum
description: Protocols: Whole body tissue samples of Mexican axolotl salamanders (Ambystoma mexicanum) at the following developmental stages: 1. Embryo at the tail bud stage 2. Newly hatched larva 3. Larva at the limb bud stage 4. Juvenile at 8.5 centimeters 5. Adult were pooled Total RNA was isolated from tissue samples using variations of guanidinium-based protocols (MacLeod et. al., 1996; Chomczynski P and Sacchi N, 1987). All tissues were considered normal and careful efforts were in place to minimize any distortion of transcript levels caused by the collection procedure. RNA quantity, purity, and integrity of both the individual samples and the resulting pool were determined with an Agilent 2100 Bioanalyzer using the Eukaryotic Total RNA nano series II analysis kit. RNA was poly-A selected and used for Illumina random priming directional library prep using the Illumina mRNA-Seq Kit (Part #RS-100-0801).
attributes.tag: ENA first public
attributes.value: 2018-07-29
attributes.tag: ENA last update
attributes.value: 2017-10-06
attributes.tag: External Id
attributes.value: SAMEA104337969
attributes.tag: INSDC center alias
attributes.value: Bioinformatics Core, Memorial Sloan Kettering Cancer Center
attributes.tag: INSDC center name
attributes.value: Bioinformatics Core, Memorial Sloan Kettering Cancer Center
attributes.tag: INSDC first public
attributes.value: 2018-07-29T17:02:18Z
attributes.tag: INSDC last update
attributes.value: 2017-10-06T09:34:47Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6092:ambystoma mexicanum tissue pool
attributes.tag: age
attributes.value: Mixed age (day 1 to 1.5 years)
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: axolotl
attributes.tag: developmental stage
attributes.value: Mixed developmental stage
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: whole organism
attributes.tag: sample name
attributes.value: E-MTAB-6092:ambystoma mexicanum tissue pool
attributes.tag: sex
attributes.value: Mixed sex
attributes.tag: specimen with known storage state
attributes.value: Fresh specimen