title: JDM-RN-15-1127-MR
organism: Homo sapiens
description: Protocols: CD19+ B cells were sorted from freeze-thawed peripheral blood mononuclear cells (PBMC) by flow cytometry (FACS Aria III), using CD19 PE-Cy7 (BioLegend). DAPI was used to exclude dead cells. Sorted B cell RNA was extracted using the Arcturus PicoPure RNA Isolation Kit. Samples were processed using Illumina's TruSeq Stranded mRNA LT sample preparation kit (p/n RS-122-2101) according to manufacturer's instructions.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-01-03T17:03:12Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2017-03-24T14:16:57Z
attributes.tag: External Id
attributes.value: SAMEA103939858
attributes.tag: INSDC center name
attributes.value: Genetics and Genomic Medicine UCL GOS Institute of Child Health
attributes.tag: INSDC first public
attributes.value: 2019-01-03T17:03:12Z
attributes.tag: INSDC last update
attributes.value: 2017-03-24T14:16:57Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-5616:JDM-RN-15-1127-MR
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: B cell
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: juvenile dermatomyositis
attributes.tag: individual
attributes.value: 15
attributes.tag: organism part
attributes.value: blood
attributes.tag: sample name
attributes.value: E-MTAB-5616:JDM-RN-15-1127-MR
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: female