title: E-MTAB-3063:LM_rep1
organism: Hordeum vulgare subsp. vulgare
description: Protocols: Four-week-old barley cv Barke plants were used for these experiments. For the RNA- seq analyses, the first true leaf (leaf 1) was used for the treatments and leaves 2 and 3 were pooled and harvested as the systemic tissue. Hordeum vulgare L. (barley) cultivar Barke seeds were sterilized in 1% sodium hypochlorite for 5 minutes, rinsed three times for 10 min with sterilized water, and then sown on fertilized soil (Einheitserde Classic of the Bayerische Gaertnereigenossenschaft, Munich, Germany). The plants were grown in the green house under the additional lights HQI-TS 400W/D (Osram, Munich, Germany) in 12 hour light/12 hour dark cycles at 70% relative humidity and were kept at 24◦C during the day and 20◦C during the night. The first true leaf (leaf 1) of four-week-old barley plants was syringe-infiltrated with 10 mM MgCl2 (Mock), and harvested 5 days after treatment, was used as Local Mock-treated samples (LM). Nucleospin RNA plant kit (Macherey-Nagel GmbH, Dueren, Germany) Illumina TruSeq RNA Sample preparation v2
attributes.tag: ENA checklist
attributes.value: ERC000011
attributes.tag: INSDC center alias
attributes.value: Helmholtz Zentrum Muenchen, Department of Environmental Sciences, Institute of Biochemical Plant Pathology
attributes.tag: INSDC center name
attributes.value: Helmholtz Zentrum Muenchen, Department of Environmental Sciences, Institute of Biochemical Plant Pathology
attributes.tag: INSDC first public
attributes.value: 2014-11-24T17:01:34Z
attributes.tag: INSDC last update
attributes.value: 2014-11-13T14:39:20Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: SRA accession
attributes.value: ERS604995
attributes.tag: Sample Name
attributes.value: ERS604995
attributes.tag: age
attributes.value: 4
attributes.tag: alias
attributes.value: E-MTAB-3063:LM_rep1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cultivar
attributes.value: Barke
attributes.tag: growth condition
attributes.value: green house study
attributes.tag: infect
attributes.value: mock
attributes.tag: organism
attributes.value: Hordeum vulgare
attributes.tag: organism part
attributes.value: leaf
attributes.tag: sampling site
attributes.value: local leaf at site of infection
attributes.tag: title
attributes.value: LM_rep1
attributes.tag: sub_species
attributes.value: vulgare