title: Sample 58
organism: Gallus gallus
description: Protocols: To obtain tissue samples at 2, 6, and 10 days post inoculation, approximately one-third of the birds were euthanized with sodium pentobarbital solution at each of the three time points. The Harderian gland was harvested and placed into RNAlater® solution (ThermoFisher Scientific, Waltham, Massachusetts) for short-term storage. Within a week the samples were removed from RNAlater and placed into a -80°C freezer for long-term storage. RNA was isolated from the Harderian gland using the RNAqueous® kit (ThermoFisher Scientific, Waltham, Massachusetts) and samples were then DNase treated using the DNA-free™ kit (ThermoFisher Scientific, Waltham, Massachusetts). The quality of all samples was validated (RNA quality number>8.0) using the Fragment Analyzer™ (Advanced Analytical Technologies, Ankeny, Iowa). From the same RNA isolate, two cDNA libraries were made (technical replicates) A 500 ng input for each technical replicate was utilized to construct the cDNA library using TruSeq® RNA Sample Preparation v2 Guide (Illumina, San Diego, California) using the high throughput protocol. Following, cDNA library validation using the Fragment Analyzer™ (Advanced Analytical Technologies, Ankeny, Iowa).
attributes.tag: ENA first public
attributes.value: 2017-10-30
attributes.tag: ENA last update
attributes.value: 2017-09-18
attributes.tag: External Id
attributes.value: SAMEA104306053
attributes.tag: INSDC center alias
attributes.value: Iowa State University
attributes.tag: INSDC center name
attributes.value: Iowa State University
attributes.tag: INSDC first public
attributes.value: 2017-10-30T17:01:59Z
attributes.tag: INSDC last update
attributes.value: 2017-09-18T17:25:12Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6038:Sample 58
attributes.tag: age
attributes.value: 23
attributes.tag: breed
attributes.value: Fayoumi
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: chicken
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: individual
attributes.value: HG1562_2
attributes.tag: organism part
attributes.value: Harderian gland
attributes.tag: phenotype
attributes.value: resistant to Newcastle disease virus infection
attributes.tag: replicate
attributes.value: 2
attributes.tag: sample name
attributes.value: E-MTAB-6038:Sample 58
attributes.tag: sex
attributes.value: female