title: Culture 1
organism: Mus musculus
description: Protocols: P19 embryonal carcinoma cells were cultured in DMEM with 10% fetal calf serum. The mouse P19ECs were obtained from ATCC and were cultured in DMEM, 10% fetal 655 calf serum, 2mM L-glutamine (Gibco) and 50 ug/ml penicillin/streptomycin (Gibco) P19ECs were stably transfected with AP2gamma. Cells were harvested and crosslinked with 1% formaldehyde. Phenol Chloroform extraction Performed ChIP according to: Magnusdottir, E. et al. Epidermal terminal differentiation depends on B lymphocyte912 induced maturation protein-1. Proceedings of the National Academy of Sciences of the United States of America 104, 14988-93 (2007). Libraries were generated using Nugene Ovation Ultralow Library Kit.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2013-07-17T17:01:01Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T16:21:53Z
attributes.tag: External Id
attributes.value: SAMEA2062613
attributes.tag: INSDC center name
attributes.value: Biomedical Center, University of Iceland
attributes.tag: INSDC first public
attributes.value: 2013-07-17T17:01:01Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T16:21:53Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-1600:Culture 1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: P19EC
attributes.tag: cell type
attributes.value: epiblast
attributes.tag: common name
attributes.value: house mouse
attributes.tag: phenotype
attributes.value: wild type
attributes.tag: sample name
attributes.value: E-MTAB-1600:Culture 1
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: specimen with known storage state
attributes.value: fresh specimen