title: A1502_087_P2
organism: soil metagenome
description: This study was carried out in the agricultural region of Itasy situated in the Highlands of Madagascar and submitted to a tropical altitude climate with MAT of 18.4�C and MAP of 1319 mm. Hillsides (locally called �tanety�) of Highlands are dominated by ferrallitic soils or Ferralsols according to the FAO classification and are subjected to a hot rainy season from November to April and a cold dry season from May to October. In April 2015, i.e. at the end of the cropping season, soil samples from 49 agricultural parcels managed by smallholder famers and 4 unexploited fields were sampled in the municipality of Imerintsiatosika (18� 59_ 00_ S, 47� 19_ 00_ E). The agricultural plots were submitted to 3 land use types (subsistence crops, agroforestry and forestry) and smallholder farmers conducted different agricultural practices.In each plot, six soil cores, 0-10 cm depth, were sampled using a metallic cylinder. One core was used to measure the soil bulk density and other physicochemical parameters. The remaining 5 soil cores were pooled, sieved (2 mm), and coarse plant debris were removed. Composite samples were maintained at 4�C for one week, prior to further fresh soil incubations and analyses.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-01-09T17:03:21Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2017-03-29T17:36:55Z
attributes.tag: External Id
attributes.value: SAMEA103949564
attributes.tag: INSDC center name
attributes.value: INRA-CMSE
attributes.tag: INSDC first public
attributes.value: 2019-01-09T17:03:21Z
attributes.tag: INSDC last update
attributes.value: 2017-03-29T17:36:55Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: MadaTR_2015_A1502_087_P2_18S
attributes.tag: collection date
attributes.value: 2015-04
attributes.tag: environment (biome)
attributes.value: terrestrial biome
attributes.tag: environment (feature)
attributes.value: anthropogenic geographic feature
attributes.tag: environment (material)
attributes.value: soil
attributes.tag: geographic location (country and/or sea)
attributes.value: Madagascar
attributes.tag: geographic location (depth)
attributes.value: 0,1
attributes.tag: geographic location (elevation)
attributes.value: 1334
attributes.tag: geographic location (latitude)
attributes.value: -18,9481
attributes.tag: geographic location (longitude)
attributes.value: 47,34615
attributes.tag: investigation type
attributes.value: metagenome
attributes.tag: multiplex identifiers
attributes.value: CGCGTATACA
attributes.tag: nucleic acid extraction
attributes.value: DNA was extracted using GnS-GII protocol as described in Terrat et al , 2012
attributes.tag: pcr primers
attributes.value: FR1 (5-ANCCATTCAATCGGTANT-3) FF390 (5-CGATAACGAACGAGACCT-3)
attributes.tag: project name
attributes.value: CAMMiSolE_Itasy
attributes.tag: sample name
attributes.value: MadaTR_2015_A1502_087_P2_18S
attributes.tag: sample volume or weight for DNA extraction
attributes.value: 2
attributes.tag: scientific_name
attributes.value: soil metagenome
attributes.tag: sequencing method
attributes.value: Illumina-MiSeq
attributes.tag: soil environmental package
attributes.value: soil
attributes.tag: target gene
attributes.value: 18S rRNA gene