title: Hadronyche formidabilis
organism: Hadronyche formidabilis
description: Nucleic acid sequences and peptide sequences from venom-gland preparations from the Australian funnel-web spider Hadronyche formidabilis.
attributes.tag: ENA first public
attributes.value: 2018-07-28
attributes.tag: ENA last update
attributes.value: 2016-07-29
attributes.tag: External Id
attributes.value: SAMEA4359021
attributes.tag: INSDC center alias
attributes.value: Institute for Molecular Biosciences
attributes.tag: INSDC center name
attributes.value: Institute for Molecular Biosciences
attributes.tag: INSDC first public
attributes.value: 2018-07-28T17:02:53Z
attributes.tag: INSDC last update
attributes.value: 2016-07-29T06:42:46Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: IMB_RL7_HFORVD1
attributes.tag: amount or size of sample collected
attributes.value: 4 venom glands
attributes.tag: broker name
attributes.value: Bioinformatics Resources Australia - EMBL
attributes.tag: collection date
attributes.value: 2012
attributes.tag: geographic location (country and/or sea)
attributes.value: Australia
attributes.tag: lat lon
attributes.value: 20.9175738 S 142.70279559 E
attributes.tag: sample material processing
attributes.value: All spiders used for complementary DNA (cDNA) library construction were first anesthetized using CO2, frozen at â80Â°C for 10 min, and then dissected at 4Â°C. To access the venom gland, the chelicerr ae were first removed from the base of the structure (towards the carapace). Once separated, each chelicera was individually cut from the ventral side up to the base of the fang in order to approach the venom gland from underneath. The cheliceral muscle that surrounds the venom gland was detached to expose the isolated venom gland. Dissected venom glands were placed immediately in TRIzolÂ® reagent (Invitrogen) to be processed.
attributes.tag: sample name
attributes.value: IMB_RL7_HFORVD1