title: DNA sample isolated from microbiological community inhabiting weathered Kupferschiefer black shale
organism: metagenome
description: Metagenomic study performed on single environmental DNA sample sequenced on Ion Proton sequencer
attributes.tag: ENA first public
attributes.value: 2019-01-09
attributes.tag: ENA last update
attributes.value: 2017-06-26
attributes.tag: External Id
attributes.value: SAMEA104140799
attributes.tag: INSDC center alias
attributes.value: INSTITUTE OF BIOCHEMISTRY AND BIOPHYSICS, POLISH ACADEMY OF SCIENCES
attributes.tag: INSDC center name
attributes.value: INSTITUTE OF BIOCHEMISTRY AND BIOPHYSICS, POLISH ACADEMY OF SCIENCES
attributes.tag: INSDC first public
attributes.value: 2019-01-09T17:03:22Z
attributes.tag: INSDC last update
attributes.value: 2017-06-26T10:58:10Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: WBS_sample1
attributes.tag: collection date
attributes.value: 2015
attributes.tag: environment (biome)
attributes.value: ENVO:01000246
attributes.tag: environment (feature)
attributes.value: ENVO:00000076
attributes.tag: environment (material)
attributes.value: ENVO:00002056
attributes.tag: geographic location (country and/or sea)
attributes.value: Poland
attributes.tag: geographic location (depth)
attributes.value: 777
attributes.tag: geographic location (elevation)
attributes.value: 620
attributes.tag: geographic location (latitude)
attributes.value: 51.434015
attributes.tag: geographic location (longitude)
attributes.value: 16.156069
attributes.tag: investigation type
attributes.value: metagenome
attributes.tag: library construction method
attributes.value: Isolated DNA was used to prepare barcoded library with Ion Xpress™ Plus Fragment Library Kit (Thermo Fisher) according to manufacturer's manual. Library was subjected to clonal amplification on Ion One Touch 2 system (Thermo Fisher) using Ion PI™ Template OT2 200 Kit v2 (Thermo Fisher) and sequenced on Ion Proton sequencer using Ion PI™ Sequencing 200 Kit v2 (Thermo Fisher) according to manufacturer's manual.
attributes.tag: library reads sequenced
attributes.value: 6645876
attributes.tag: nucleic acid extraction
attributes.value: Zhou, J., Bruns, M.A., and Tiedje, J.M. (1996) DNA recovery from soils of diverse composition. Appl Environ Microbiol 62: 316-322
attributes.tag: project name
attributes.value: The long-term oxidative weathering of fossil organic matter and sulfide minerals of the subterrestrial Kupferschiefer black shale by lithobiontic microbial community - metaomics field studies
attributes.tag: sample name
attributes.value: WBS_sample1
attributes.tag: sample volume or weight for DNA extraction
attributes.value: 100
attributes.tag: sediment environmental package
attributes.value: sediment
attributes.tag: sequencing method
attributes.value: Ion Torrent