title: 10g DNA Extracted Directly From Sediment
organism: marine sediment metagenome
description: Sediment was collected from Cadillac Avenue located on Dauphin Island, Alabama, USA on August 22, 2011. Environmental DNA was extracted directly from 10 g of sediment using the MoBio Laboratories PowerSoil DNA Isolation Kit. Extractions were performed in duplicate and serve as biological replicates. Amplification of the eukaryotic specific hypervariable V9 region of the 18S small subunit ribosomal RNA (SSU rRNA) gene was done in duplicate and serve as technical replicates. Sequencing was performed on an Illumina HiSeq 2500 at the Genomic Services Laboratory at Hudson Alpha Institute for Biotechnology (Huntsville, Alabama). Biological and technical replicates were kept separate through the Operational Taxonomic Unit (OTU) clustering. Biological replicates were used to observe variation while technical replicates served as a quality control measure.
attributes.tag: collection_date
attributes.value: 2011-08-22
attributes.tag: geo_loc_name
attributes.value: USA: Cadillac Avenue located on Dauphin Island, Alabama
attributes.tag: lat_lon
attributes.value: 30.253167 N 88.136317 W
attributes.tag: salinity
attributes.value: 23 ppt
attributes.tag: temperature
attributes.value: NA
attributes.tag: depth
attributes.value: 0-3 cm
attributes.tag: elev
attributes.value: 0
attributes.tag: env_biome
attributes.value: marine
attributes.tag: env_feature
attributes.value: bay
attributes.tag: env_material
attributes.value: sediment
attributes.tag: BioSampleModel
attributes.value: MIMS.me
attributes.tag: BioSampleModel
attributes.value: MIGS/MIMS/MIMARKS.sediment