title: D0_B
organism: Homo sapiens
description: Protocols: Human peripheral-blood monocytes were obtained from healthy donors. CD14+ monocytes were purified using CD14 microbeads with a light-scattering (LS) column according to the manufacturer's instructions (Miltenyi Biotec, Bergisch Gladbach, Germany). CD14+ were treated with CSF1 at 100 ng/mL during 3 days CD14+ were treated with CSF1 at 100 ng/mL and QVD at 50 μM during 3 days CD14+ were treated with GM-CSF at 100 ng/mL during 3 days RNA were extracted with the RNeasy mini kit (QIAGEN) SureSelect Automated strand specific RNA library prep. mRNA library prep for Illumina paired-end multiplexed sequencing.
attributes.tag: ENA first public
attributes.value: 2018-12-30
attributes.tag: ENA last update
attributes.value: 2017-05-22
attributes.tag: External Id
attributes.value: SAMEA104091391
attributes.tag: INSDC center alias
attributes.value: Inserm U1170 - Institut Gustave Roussy
attributes.tag: INSDC center name
attributes.value: Inserm U1170 - Institut Gustave Roussy
attributes.tag: INSDC first public
attributes.value: 2018-12-30T17:01:42Z
attributes.tag: INSDC last update
attributes.value: 2017-05-22T15:33:35Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-5752:D0_B
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: CD14-positive, CD16-negative classical monocyte
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: normal
attributes.tag: individual
attributes.value: B
attributes.tag: organism part
attributes.value: blood
attributes.tag: sample name
attributes.value: E-MTAB-5752:D0_B