title: Wildtype anti-H4K20me3 ChIP
organism: Mus musculus
description: Protocols: mES cells (E14.1) were cultured on plates coated with 0.1% gelatin (Millipore) in high-glucose Dulbecco's modiﬁed Eagle's medium (DMEM; HyClone) supplemented with 15% ES cell-qualiﬁed fetal bovine serum (Gibco), 1% penicillin and streptomycin (Gibco), 2 mM L-glutamine (Gibco), 0.1 mM non-essential amino acids (NEAAs; Gibco), 0.1 mM 2-mercaptoethanol (Gibco) and 1000 units/ml of LIF (Chemicon). Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. Libraries were prepared for sequencing using standard Illumina protocols at BGI-Shenzhen (sequencing service provider).
attributes.tag: ENA first public
attributes.value: 2018-12-30
attributes.tag: ENA last update
attributes.value: 2017-08-22
attributes.tag: External Id
attributes.value: SAMEA104222293
attributes.tag: INSDC center alias
attributes.value: Department of Bioinformatics,Tongji University
attributes.tag: INSDC center name
attributes.value: Department of Bioinformatics,Tongji University
attributes.tag: INSDC first public
attributes.value: 2018-12-30T17:01:43Z
attributes.tag: INSDC last update
attributes.value: 2017-08-22T11:59:51Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-5985:Wildtype anti-H4K20me3 ChIP
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: E14.1
attributes.tag: cell type
attributes.value: embryonic stem cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: embryo
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: sample name
attributes.value: E-MTAB-5985:Wildtype anti-H4K20me3 ChIP