title: OG89 sample (Oryza glumaepatula STEUD.)
organism: Oryza glumipatula
attributes.tag: isolate
attributes.value: Fourth generation of single descent seed from an Oryza glumaepatula acession tolerant to aluminium OG89
attributes.tag: age
attributes.value: 31 days since germination
attributes.tag: dev_stage
attributes.value: Seedlings with two developed leaves
attributes.tag: geo_loc_name
attributes.value: Venezuela:Guarico State
attributes.tag: tissue
attributes.value: roots
attributes.tag: collected_by
attributes.value: Lentini and colaborators-Icesi University from Colombia and Central University of Venezuela
attributes.tag: collection_date
attributes.value: 2005
attributes.tag: growth_protocol
attributes.value: Seeds were grow on distillated water at 30 C degrees in dark during 10 days, then transferred to hydroponic culture solution Kimura B supplemented with Arnon micronutrients (Chen el at. 2006) at pH6,0 where they grow for 7 days. After that, the pH was gradually lowered to 4,0 during 4 more days. The half of the plants were then subjected to aluminum treatment of 100 uM Al3+ free and the other half to 0 uM Al3+, during a period of 10 days. Roots samples were collected after those 10 days of treatment.
attributes.tag: treatment
attributes.value: 100uM Al 3+ free and 0uM Al 3+ free in an the hydroponic culture solution KimuraB with Arnon micronutrients (Chen et al 2006), at pH 4,0
attributes.tag: BioSampleModel
attributes.value: Plant