title: BAL_HDM [Chris_G_A3_9_WB17_1_S207_L006]
organism: Mus musculus
attributes.tag: source_name
attributes.value: BAL_HDM
attributes.tag: mouse model
attributes.value: House dust mite (HDM) allergy
attributes.tag: mouse strain
attributes.value: C57BL/6/J
attributes.tag: condition
attributes.value: Disease
attributes.tag: infection protocol
attributes.value: For HDM allergy induction via mucosal exposure, female C57BL/6/J mice were administered 25 μg HDM (Greer) in 25 μl PBS or 25 μl PBS control intranasally under isofluorane anaesthesia 5 days per week for 3 weeks. Whole blood and bronchoalveolar lavage (BAL) samples were collected from individual HDM and PBS control treated mice 24 hours after final allergen challenge. For BAL, airways were flushed 3 times with 1 ml of chilled 5 mM EDTA (Invitrogen, Thermo Fisher) in PBS via a tracheal cannula, before resting mice with 1 ml EDTA/PBS in the lungs for 5 minutes and lavaging a further 3 times with 1 ml EDTA/PBS.
attributes.tag: tissue
attributes.value: Bronchoalveolar lavage