title: Sample 1
organism: Trichonephila clavipes
description: Protocols: A total of 10 N. clavipes spiders were collected in a georeferenced site from the campus of the University of São Paulo State at Rio Claro, SP, Southeast Brazil. The silk glands were dissected and stored in the presence of RNAlater stabilization reagent (ThermoFisher Scientific) for RNA preservation until use. cDNA fragments subsequently were through an end-repair process, the addition of a single A base and ligation of the adaptors. The products were purified and enriched with 15 cycles of PCR (with a size distribution of ~200–250 bp) to create the final cDNA library.
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-01-09T17:03:20Z
attributes.tag: organism
attributes.value: Nephila clavipes
attributes.tag: organism
attributes.value: Nephila clavipes
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2017-02-08T12:27:46Z
attributes.tag: scientific_name
attributes.value: Nephila clavipes
attributes.tag: organism part
attributes.value: silk gland
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-01-09
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2017-02-08