title: flag03_a
organism: Escherichia coli
description: Protocols: EPEC E2348/69 hfq-Flag strain was grown in 2 set of conditions: For non-activating conditions, the culture was grown overnight in LB, without shaking. For activating conditions, a non-activating culture was diluted 1:100 into Dulbecco's modified Eagle medium (DMEM, Biological Industries) and grown to reach OD600 of 0.3. in activating- conditions:growth on DMEM at 37°C to mid exponential growth phase (e.g., OD600=0.3). In these conditions EPEC strongly expresses its major virulence components, T3SS and BFP, mimicking infection RIL-seq experiments were performed as described in Melamed et al 2018, PMID: 29215635 , each repeated three times. RNAtag-Seq protocol (Shishkin et al., 2015)
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2021-01-29T04:14:02Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-02-18T16:02:18Z
attributes.tag: External Id
attributes.value: SAMEA6567108
attributes.tag: INSDC center name
attributes.value: Department of Microbiology and Molecular Genetics, Institute for Medical Research Israel-Canada, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem 9112102, Israel. MIT Physics of Living Systems, Department of Physics, Massachusetts Institute of Technology
attributes.tag: INSDC first public
attributes.value: 2021-01-29T04:14:02Z
attributes.tag: INSDC last update
attributes.value: 2020-02-18T16:02:18Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8806:flag03_a
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: genotype
attributes.value: hfq-flag
attributes.tag: growth condition
attributes.value: DMEM
attributes.tag: organism part
attributes.value: whole organism
attributes.tag: sample name
attributes.value: E-MTAB-8806:flag03_a
attributes.tag: scientific_name
attributes.value: Escherichia coli
attributes.tag: strain
attributes.value: O127:H6 str. E2348/69