title: cell_culture_3
organism: Homo sapiens
description: Protocols: The HEK293_M2 + RHOXF2 stable cell line (PB-TGcMV-Neo) were seeded in 10cm-dishes, cultivated 24 hours in the presence or absence of doxycycline (2microgram/ml final) before been treated with 40nM of mitomycin C or DMSO (control) during a 2-days period. Total RNA was extracted (RNeasy Mini Kit - Qiagen), mRNA-focused libraries was generated from 1ug of total RNA (Illumina TruSeq RNA Sample Preparation Kit V.2). Cells were treated with 40nM of mitomycin C or DMSO (control) during a 2-days period. Total RNA was extracted (RNeasy Mini Kit Qiagen) mRNA-focused libraries was generated from 1ug of total RNA (Illumina TruSeq RNA Sample Preparation Kit V.2)
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2014-05-15T17:02:21Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T17:21:50Z
attributes.tag: External Id
attributes.value: SAMEA2464849
attributes.tag: INSDC center name
attributes.value: University of Toronto
attributes.tag: INSDC first public
attributes.value: 2014-05-15T17:02:21Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T17:21:50Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-2497:cell_culture_3
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: HEK293
attributes.tag: common name
attributes.value: human
attributes.tag: genotype
attributes.value: RHOXF2 not overexpressed in HEK293
attributes.tag: sample name
attributes.value: E-MTAB-2497:cell_culture_3
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: specimen with known storage state
attributes.value: fresh specimen