title: Non-Podocytes_tecrp1
organism: Mus musculus
description: Protocols: Glomeruli were isolated using the beads perfusion method and glomerular singe cell solution was prepared as previously described (see Boerries M et al., Kidney Int. 2013 Jun;83(6):1052-64: Materials and Methods → Glomerular preparation & Podocyte preparation). Glomeruli were isolated using the beads perfusion method and glomerular singe cell solution was prepared as previously described (see Boerries M et al., Kidney Int. 2013 Jun;83(6):1052-64: Materials and Methods → Glomerular preparation & Podocyte preparation). RNA was extracted using the chloroform/phenol method with DNAase digestion (see Boerries M et al., Kidney Int. 2013 Jun;83(6):1052-64: Materials and Methods → RNA preparation). The Illumina TruSeq stranded total RNA sample preparation LS protocol was used for directional, polyA+ library preparation.
attributes.tag: ENA first public
attributes.value: 2019-01-09
attributes.tag: ENA last update
attributes.value: 2017-02-08
attributes.tag: External Id
attributes.value: SAMEA80827168
attributes.tag: INSDC center alias
attributes.value: Huber lab Medical Center - University of Freiburg Department of Medicine IV (Specialties: Nephrology and Primary Care)
attributes.tag: INSDC center name
attributes.value: Huber lab Medical Center - University of Freiburg Department of Medicine IV (Specialties: Nephrology and Primary Care)
attributes.tag: INSDC first public
attributes.value: 2019-01-09T17:03:20Z
attributes.tag: INSDC last update
attributes.value: 2017-02-08T10:47:03Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-5457:Non-Podocytes_tecrp1
attributes.tag: age
attributes.value: 10 weeks
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: Non-Podocytes glomerular cells
attributes.tag: common name
attributes.value: house mouse
attributes.tag: genetic modification
attributes.value: Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J x hNPHS2Cre
attributes.tag: sample name
attributes.value: E-MTAB-5457:Non-Podocytes_tecrp1
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: mixed genetic background (Sv129/C57Bl6/ICR)