title: run3.7
organism: freshwater metagenome
description: 40394-metalimnion
attributes.tag: ENA first public
attributes.value: 2018-04-29
attributes.tag: ENA last update
attributes.value: 2017-08-23
attributes.tag: External Id
attributes.value: SAMEA104225214
attributes.tag: INSDC center alias
attributes.value: UU
attributes.tag: INSDC center name
attributes.value: Uppsala University
attributes.tag: INSDC first public
attributes.value: 2018-04-29T17:02:57Z
attributes.tag: INSDC last update
attributes.value: 2017-08-23T16:29:14Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: run3.7
attributes.tag: collection date
attributes.value: 2014-08-05
attributes.tag: common name
attributes.value: freshwater metagenome
attributes.tag: environment (biome)
attributes.value: aquatic
attributes.tag: environment (feature)
attributes.value: freshwater
attributes.tag: environment (material)
attributes.value: small lake
attributes.tag: geographic location (country and/or sea)
attributes.value: Sweden
attributes.tag: geographic location (depth)
attributes.value: 6-Dec
attributes.tag: geographic location (elevation)
attributes.value: 15
attributes.tag: geographic location (latitude)
attributes.value: 59.8376
attributes.tag: geographic location (longitude)
attributes.value: 18.6203
attributes.tag: investigation type
attributes.value: mimarks-survey
attributes.tag: lake stratification
attributes.value: stratified
attributes.tag: layer sampled
attributes.value: metalimnion
attributes.tag: multiplex identifiers
attributes.value: GTAGAGAG-TAGATCGC
attributes.tag: pcr conditions
attributes.value: initial denaturation step at 95 �C for 30 s, followed by 20 cycles of dissociation at 95 �C for 10 s, annealing at 53 �C for 30 s extension at 72 �C for 30 s, with a final extension of 2 min at 72 �C. then second round: initial denaturation step at 95 �C for 30 s, followed by 15 cycles of dissociation at 95 �C for 10 s, annealing at 66 �C for 30 s, extension at 72 �C for 30 s, with a final extension of 2 min at 72 �C
attributes.tag: pcr primers
attributes.value: CCTACGGGNGGCWGCAG-GACTACNVGGGTATCTAATCC
attributes.tag: ph
attributes.value: 8.19
attributes.tag: project name
attributes.value: Lake Erken SSU rRNA gene amplicon timeseries
attributes.tag: sample name
attributes.value: run3.7
attributes.tag: sample storage temperature
attributes.value: -80
attributes.tag: sample volume or weight for DNA extraction
attributes.value: 0.4
attributes.tag: sequencing method
attributes.value: Illumina Miseq PE
attributes.tag: target gene
attributes.value: SSU rRNA
attributes.tag: target subfragment
attributes.value: V3-V4
attributes.tag: total depth of water column
attributes.value: 20
attributes.tag: total nitrogen
attributes.value: 697.44
attributes.tag: total phosphorus
attributes.value: 0.58597275
attributes.tag: water environmental package
attributes.value: water