title: CreWT_ExVivo_Untreated_3
organism: Mus musculus
description: Protocols: FACS sorted subsets were either left untouched (Ex vivo) or cultured for 6h with 1mg/ml LPS and 10mg/ml anti-IgM, before DNA extraction. ~40,000 cells were pelleted by centrifuging for 5 min at 4 °C at 300 x g. After centrifugation, the pellet was carefully resuspended in the transposase reaction mix (12.5 ml 2xTD buffer, 2 ml TDE1 (Illumina) and 10.25 ml nuclease-free water, 0.25 µl 5% Digitonin (Sigma)) for 30 min at 37 °C. Per manufacturer's instruction Per manufacturer's instruction
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-12-31T17:02:32Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-12-21T14:19:54Z
attributes.tag: External Id
attributes.value: SAMEA5198351
attributes.tag: INSDC center name
attributes.value: Bering Limited
attributes.tag: INSDC first public
attributes.value: 2018-12-31T17:02:32Z
attributes.tag: INSDC last update
attributes.value: 2018-12-21T14:19:54Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7525:CreWT_ExVivo_Untreated_3
attributes.tag: age
attributes.value: 8 to 12
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: B cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: Mb1+/cre
attributes.tag: individual
attributes.value: 4-4_ct_ev_1
attributes.tag: organism part
attributes.value: spleen
attributes.tag: phenotype
attributes.value: CD19+CD21hiCD24hi
attributes.tag: sample name
attributes.value: E-MTAB-7525:CreWT_ExVivo_Untreated_3
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: C57BL/6