title: SE_5D
organism: Arabidopsis thaliana
description: Immature zygotic embryos collected after 5 d of inoculation on B5 medium supplemented with 5 uM 2, 4-dichlorophenoxyacetic acid Protocols: Somatic embryos were induced on B5 medium supplemented with 5 uM 2, 4-dichlorophenoxyacetic acid (Gaj, 2001). In vitro cultures were maintained at 24C under a 16 h/ 8 h (light/dark) cycle in a culture room. Samples were collected after 5 d of in vitro culture. Approximately 700 embryogenic cultures induced at each time-point were pooled together to extract total RNA. Total RNA was extracted using the RNeasy® Plant Mini Kit (Qiagen, UK) according to the manufacturers protocol. The cDNA libraries were constructed in accordance with the Illumina TruSeq RNA sample preparation guide v2 for Illumina paired-end multiplexed sequencing.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2016-01-21T17:01:32Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T17:18:22Z
attributes.tag: External Id
attributes.value: SAMEA2421742
attributes.tag: INSDC center name
attributes.value: School of Agriculture, Policy and Development, The University of Reading, UK
attributes.tag: INSDC first public
attributes.value: 2016-01-21T17:01:32Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T17:18:22Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-2403:SE_5D
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: thale cress
attributes.tag: organism part
attributes.value: somatic embryo
attributes.tag: sample name
attributes.value: E-MTAB-2403:SE_5D
attributes.tag: scientific_name
attributes.value: Arabidopsis thaliana
attributes.tag: specimen with known storage state
attributes.value: frozen specimen