title: MuYFPplus2
organism: Mus musculus
description: Protocols: FACS sorting experiments for gene expression analyses were performed on a BD Aria III (BD Biosciences). Cells were immediately sorted in RLT plus lysis buffer (Qiagen) with beta-mercaptoethanol and placed on dry ice. RNA was isolated using the RNeasy Plus Micro Kit (Qiagen) according to instructions by the manufacturer. cDNA libraries were prepared SMART-Seq v4 Ultra Low Input RNA kit (Clonentech) following the manufacturer's protocol.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-07-26T17:01:55Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-07-11T13:11:15Z
attributes.tag: External Id
attributes.value: SAMEA4786353
attributes.tag: INSDC center name
attributes.value: Translational Research Center for Gastrointestinal Disorders (TARGID) Department of Chronic Diseases, Metabolism and Ageing KU Leuven Belgium
attributes.tag: INSDC first public
attributes.value: 2018-07-26T17:01:55Z
attributes.tag: INSDC last update
attributes.value: 2018-07-11T13:11:15Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6977:MuYFPplus2
attributes.tag: age
attributes.value: 35
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: macrophage
attributes.tag: common name
attributes.value: house mouse
attributes.tag: genotype
attributes.value: Cx3cr1CreERT2.Rosa26-LSL-YFP
attributes.tag: individual
attributes.value: 1
attributes.tag: organism part
attributes.value: muscularis externa layer of small intestine
attributes.tag: phenotype
attributes.value: YFP positive
attributes.tag: sample name
attributes.value: E-MTAB-6977:MuYFPplus2
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: female
attributes.tag: stimulus
attributes.value: tamoxifen
attributes.tag: strain
attributes.value: C57BL/6