title: S19_PL8
organism: Gallus gallus
description: Protocols: Tissues (HH8 node sub-regions) were isolated from transgenic-GFP embryos using 30G syringe needles in sterile molecular grade PBS. Tissues were collected into RNAlater (Invitrogen, # AM7020). For each sample, tissues were collected from thirteen to seventeen embryos. RNA was extracted using the Micro Total RNA Isolation Kit (Invitrogen, # AM1931) and concentration and quality measured using Tapestation The NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (# E6420) was used for cDNA and library synthesis
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2021-01-31T04:08:30Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-05-23T19:03:44Z
attributes.tag: External Id
attributes.value: SAMEA6853609
attributes.tag: INSDC center name
attributes.value: Cell and Development Biology, University College London
attributes.tag: INSDC first public
attributes.value: 2021-01-31T04:08:30Z
attributes.tag: INSDC last update
attributes.value: 2020-05-23T19:03:44Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-9115:S19_PL8
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: chicken
attributes.tag: developmental stage
attributes.value: HH8
attributes.tag: sample name
attributes.value: E-MTAB-9115:S19_PL8
attributes.tag: sampling site
attributes.value: Hensen's node posterior left
attributes.tag: scientific_name
attributes.value: Gallus gallus