title: E-MTAB-5499:Sample 2
organism: Pseudolactococcus piscium
attributes.tag: Alias
attributes.value: E-MTAB-5499:Sample 2
attributes.tag: Broker name
attributes.value: ArrayExpress
attributes.tag: Description
attributes.value: Protocols: LAB were grown separately in individual cultures or in mixed cultures on a glucose-containing growth medium (MRS) under microaerobic conditions at 25 °C, samples were taken at three time points (3, 5 and 11 h) RNA extraction and library construction were done analogously as in the study (Andreevskaya M et al., 2015. Appl. Environ. Microbiol. 81:3800–3811, doi: 10.1128/AEM.00320-15). Briefly, cell metabolism and RNAase activity were inhibited by the treatment with a cold 10:1 mixture of ethanol-phenol, samples were centrifuged and immediately frozen. The cells were disrupted by a mixer mill and RNA was extracted by the Rneasy Plant Mini Kit (Qiagen) with DNAase treatment. Ribosomal RNAs were omitted from the total RNA. RNA-seq libraries were produced using Ovation RNA-seq System V2 (NuGEN). The obtained dsDNA was sheared using sonication, followed by the polishing of the obtained fragments with T4 DNA polymerase and the ligation of adapters for SOLiD sequencing. Libraries were size selected.
attributes.tag: ENA checklist
attributes.value: ERC000011
attributes.tag: INSDC center name
attributes.value: DNA Sequencing and Genomics Lab Institute of Biotechnology University of Helsinki
attributes.tag: INSDC first public
attributes.value: 2018-04-27T17:03:08Z
attributes.tag: INSDC last update
attributes.value: 2017-02-22T16:18:06Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: SRA accession
attributes.value: ERS1568755
attributes.tag: Sample Name
attributes.value: ERS1568755
attributes.tag: Title
attributes.value: Sample 2
attributes.tag: batch
attributes.value: b2
attributes.tag: organism
attributes.value: Lactococcus piscium
attributes.tag: replicate
attributes.value: 2