title: deltahfqNA_c
organism: Escherichia coli
description: Protocols: EPEC E2348/69 w.t and delta-hfq strains was grown in 2 set of conditions: For non-activating conditions, the culture was grown overnight in LB, without shaking. For activating conditions, a non-activating culture was diluted 1:100 into Dulbecco's modified Eagle medium (DMEM, Biological Industries) and grown to reach OD600 of 0.3. Bacteria were harvested by centrifugation, the pellet was resuspended in TE buffer, mixed with lysosyme (Sigma Aldrich) in a final concentration of 0.9 mg/ml and immediately frozen in liquid nitrogen. The frozen pellet was subjected to two cycles of thaw and freeze at 37°C and in liquid nitrogen, respectively, and RNA was extracted using Tri-Reagent (Sigma Aldrich) RNAtag-Seq protocol (Shishkin et al., 2015)
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2021-01-29T04:14:02Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-02-18T17:02:13Z
attributes.tag: External Id
attributes.value: SAMEA6567150
attributes.tag: INSDC center name
attributes.value: Department of Microbiology and Molecular Genetics, Institute for Medical Research Israel-Canada, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem 9112102, Israel. MIT Physics of Living Systems, Department of Physics, Massachusetts Institute of Technology
attributes.tag: INSDC first public
attributes.value: 2021-01-29T04:14:02Z
attributes.tag: INSDC last update
attributes.value: 2020-02-18T17:02:13Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8804:deltahfqNA_c
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: genotype
attributes.value: Hfq mutant
attributes.tag: growth condition
attributes.value: LB medium
attributes.tag: organism part
attributes.value: whole organism
attributes.tag: sample name
attributes.value: E-MTAB-8804:deltahfqNA_c
attributes.tag: scientific_name
attributes.value: Escherichia coli
attributes.tag: strain
attributes.value: O127:H6 str. E2348/69