title: 7-1_ct_ev_S26
organism: Mus musculus
description: Protocols: Splenic CD21hiCD24hi B cells were isolated from Mb1cre and Ahrfl/-Mb1cre mice in the remission phase of arthritis. Sorted cells were either left untouched (ex-vivo) or stimulated with LPS+anti-IgM for 6h in IMDM media. Dead cells were excluded using DAPI. Total RNA was isolated from these populations using the Picopure RNA isolation kit (ThermoFisher Scientific), according to manufacturer's instructions. 130-500ng of total RNA was fragmented followed by reverse transcription and second strand cDNA synthesis. The double strand cDNA was subjected to end repair, A base addition, adapter ligation and PCR amplification to create libraries. Libraries were evaluated by Qubit and TapeStation. Sequencing libraries were constructed with barcodes to allow multiplexing of samples in 3 lanes.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-01-12T17:02:22Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-01-02T11:57:23Z
attributes.tag: External Id
attributes.value: SAMEA5203290
attributes.tag: INSDC center name
attributes.value: Bering Limited
attributes.tag: INSDC first public
attributes.value: 2019-01-12T17:02:22Z
attributes.tag: INSDC last update
attributes.value: 2019-01-02T11:57:23Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7345:7-1_ct_ev_S26
attributes.tag: age
attributes.value: 8 to 12
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: B cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: genotype
attributes.value: Mb1+/cre
attributes.tag: organism part
attributes.value: spleen
attributes.tag: phenotype
attributes.value: wild type
attributes.tag: sample name
attributes.value: E-MTAB-7345:7-1_ct_ev_S26
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: C57BL/6