title: WT_FLAG
organism: Homo sapiens
description: Protocols: Approx 8 million adherent cells were crosslinked for 10 mins in 1% formaldehyde. Cross linking was stopped by adding glycine to 0.125M. Cells were lysed in Farnham Lysis Buffer, washed in PBS and nuclei were lysed with RIPA buffer. ZR75-1 cells were cultured in RPMI 1640 supplemented with 10% serum Cell lines were generated by retroviral transduction of ZR75-1 with vectors containing either GATA3 WT, 308 or 335 mutations. Samples were sonicated using a Diagenode XL Bioruptor to fragments of 200-400 bp. Chromatin immunoprecipitation was performed using anti-FLAG antibody bound to protein G magnetic dynabeads. DNA fragments recovered from chromatin immunoprecipitation were reapired using Klenow DNA polymerase, liganted to Illumina TruSeq adapters using T4 DNA ligase and size selected for DNA with adapters attached.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-01-18T17:02:25Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-01-08T17:31:39Z
attributes.tag: External Id
attributes.value: SAMEA5207810
attributes.tag: INSDC center name
attributes.value: Kabara Cancer Research Institute
attributes.tag: INSDC first public
attributes.value: 2019-01-18T17:02:25Z
attributes.tag: INSDC last update
attributes.value: 2019-01-08T17:31:39Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7578:WT_FLAG
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: ZR-75-1
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: invasive ductal carcinoma
attributes.tag: individual
attributes.value: wild type GATA3
attributes.tag: organism part
attributes.value: mammary gland
attributes.tag: sample name
attributes.value: E-MTAB-7578:WT_FLAG
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: female