title: RNA-seq CHX 1
organism: Homo sapiens
description: Protocols: Naive CD4+ T cells purified from the blood of healthy donors were polarized in vitro to TH1 in RPMI media with 10% FBS, 0.1% Penicillin/Streptomycin 0.1 % nonessential amino acids and 0,1% Sodium Pyruvate at 37°C and 5% CO2. For RNAseq samples, RNA was extracted using the mirVana isolation kit according to manufacturer's instructions (mirVana ThermoFisher #AM 1560) Libraries for RNAseq were prepared using Illumina TruSeq Stranded Total RNA Library Prep Kit
attributes.tag: ENA first public
attributes.value: 2019-01-20
attributes.tag: ENA last update
attributes.value: 2017-08-10
attributes.tag: External Id
attributes.value: SAMEA104209705
attributes.tag: INSDC center alias
attributes.value: INGM, National Institute of Molecular Genetics, “Romeo ed Enrica Invernizzi”, Milan, Italy
attributes.tag: INSDC center name
attributes.value: INGM, National Institute of Molecular Genetics, “Romeo ed Enrica Invernizzi”, Milan, Italy
attributes.tag: INSDC first public
attributes.value: 2019-01-20T17:02:03Z
attributes.tag: INSDC last update
attributes.value: 2017-08-10T15:52:51Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-5961:RNA-seq CHX 1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: T-helper 1 cell
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: normal
attributes.tag: organism part
attributes.value: blood
attributes.tag: protocol
attributes.value: RNA-seq
attributes.tag: sample name
attributes.value: E-MTAB-5961:RNA-seq CHX 1