title: SP_rep3
organism: Hordeum vulgare
description: Protocols: Four-week-old barley cv Barke plants were used for these experiments. For the RNA- seq analyses, the first true leaf (leaf 1) was used for the treatments and leaves 2 and 3 were pooled and harvested as the systemic tissue. Hordeum vulgare L. (barley) cultivar Barke seeds were sterilized in 1% sodium hypochlorite for 5 minutes, rinsed three times for 10 min with sterilized water, and then sown on fertilized soil (Einheitserde Classic of the Bayerische Gaertnereigenossenschaft, Munich, Germany). The plants were grown in the green house under the additional lights HQI-TS 400W/D (Osram, Munich, Germany) in 12 hour light/12 hour dark cycles at 70% relative humidity and were kept at 24◦C during the day and 20◦C during the night. The first true leaf (leaf 1) of four-week-old barley plants was syringe-infiltrated with 106 cfu/ml of Psj. Five days after the primary treatment, the next two fully expanded leaves of the treated plants (leaf 2 and 3) were harvested and used as systemic Psj pretreated samples (SP). Nucleospin RNA plant kit (Macherey-Nagel GmbH, Dueren, Germany) Illumina TruSeq RNA Sample preparation v2
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2014-11-24T17:01:34Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2014-11-13T14:39:20Z
attributes.tag: External Id
attributes.value: SAMEA3120855
attributes.tag: INSDC center name
attributes.value: Helmholtz Zentrum Muenchen, Department of Environmental Sciences, Institute of Biochemical Plant Pathology
attributes.tag: INSDC first public
attributes.value: 2014-11-24T17:01:34Z
attributes.tag: INSDC last update
attributes.value: 2014-11-13T14:39:20Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-3063:SP_rep3
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: infect
attributes.value: Pseudomonas syringae pathovar japonica
attributes.tag: organism part
attributes.value: leaf
attributes.tag: sample name
attributes.value: E-MTAB-3063:SP_rep3
attributes.tag: sampling site
attributes.value: systemic leaf not at the site of infection
attributes.tag: scientific_name
attributes.value: Hordeum vulgare