title: R1_Col_elf18_010
organism: Arabidopsis thaliana
description: Protocols: Seedlings for collection were in 24-well plates. Each well contained two seedlings; two wells and four seedlings were combined for one sample. Samples were quickly blotted dry and flash-frozen in liquid nitrogen. Arabidopsis seeds were surface-sterilized, stratified 3-5 days, grown 4 days in 22 °C, 16 h/8 h light/dark on full strength MS agar plates, 1% sucrose. 4-day-old seedlings were transferred to full strength liquid MS media, 1% sucrose, and grown for 9 more days. When plants were 13 days old, media was exchanged, and after overnight incubation elicitor was added to individual wells. Elicitor treatment was added as 400 uL 2.5x concentration in liquid MS medium to 600uL liquid MS medium, resulting in 1 mL 1x concentration. mRNA was extracted from plant lysate through pull-down of poly-A tail, as described in \BrAD-seq: Breath Adapter Directional sequencing: a streamlined, ultra-simple and fast library preparation protocol for strand specific mRNA library construction\ Townsley et al Frontiers in Plant Science 2015. dscDNA was made as in \A high-throughput method for Illumina RNA-Seq library preparation\ Kumar et al Frontiers in Plant Science 2012 Libraries were simultaneously fragmented and tagged using a hyperactive Tn-5 as in \Tn5 transposase and tagmentation procedures for massively scaled sequencing projects\ Picelli et al 2014 Genome Research and \High‐resolution Expression Profiling of Selected Gene Sets during Plant Immune Activation\ Ding et al Plant Biotechnology Journal 2020
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2021-01-29T04:16:20Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-10-21T15:22:56Z
attributes.tag: External Id
attributes.value: SAMEA7471721
attributes.tag: INSDC center name
attributes.value: UZH
attributes.tag: INSDC first public
attributes.value: 2021-01-29T04:16:20Z
attributes.tag: INSDC last update
attributes.value: 2020-10-21T15:22:56Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-9694:R1_Col_elf18_010
attributes.tag: age
attributes.value: 2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: thale cress
attributes.tag: developmental stage
attributes.value: seedling development stage
attributes.tag: ecotype
attributes.value: Col-0
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: growth condition
attributes.value: long day length regimen
attributes.tag: organism part
attributes.value: whole organism
attributes.tag: sample name
attributes.value: E-MTAB-9694:R1_Col_elf18_010
attributes.tag: scientific_name
attributes.value: Arabidopsis thaliana
attributes.tag: stimulus
attributes.value: elf18