title: c402
organism: Mus musculus
description: Protocols: The neural retinas (NR) of enucleated eyes were dissected in 1X PBS (with no RPE). The NR were then placed in 1.5ml eppendorf tubes in 1X PBS and were span down. The supernatant (PBS) was discarded. RNA was either extracted straight away or the NR were frozen down in dry ice. Once frozen, they were stored at -80ºC for long term storage.Total RNA extraction from NR was carried out using the miRNeasy Micro Kit from Qiagen (217084), following manufacturers instructions. An optional DNase step (Qiagen 79254) was also included in the protocol, following manufacturers instructions, in order to remove genomic DNA. RNA was quantified using an Agilent TapeStation (TapeStation Analysis Software A.02.01) for RNA sequencing. Extracted RNA was stored at -80ºC before sequencing. RNA was extracted using the miRNeasy Micro Kit (Cat No./ID: 217084) following manufacturer's instructions and including the optional DNase step. PolyA- Illumina's TruSeq stranded kit (RS-122-2103) was used for library prep.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-01-14T17:03:33Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-01-14T10:21:13Z
attributes.tag: External Id
attributes.value: SAMEA5216294
attributes.tag: INSDC center name
attributes.value: University College London Great Ormond Street Institute of Child Health
attributes.tag: INSDC first public
attributes.value: 2019-01-14T17:03:33Z
attributes.tag: INSDC last update
attributes.value: 2019-01-14T10:21:13Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6133:c402
attributes.tag: age
attributes.value: 21
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: genotype
attributes.value: Dicer flox/flox
attributes.tag: organism part
attributes.value: retinal neural layer
attributes.tag: phenotype
attributes.value: wild type
attributes.tag: sample name
attributes.value: E-MTAB-6133:c402
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: strain
attributes.value: C57BL/6