title: RN7_95
organism: soil metagenome
description: This study was carried out in the Highlands of Madagascar, usually characterized as being above 900 m above sea level. Hillsides (locally called �tanety�) are dominated by ferrallitic soils subjected to tropical altitude climate with a hot rainy season from November to April and a cold dry season from May to October. In May 2014, soil samples from 41 plots were sampled under similar vegetation (herbaceous savannah mainly composed of the grass Aristida sp., Poaceae). Plots were located along 3 different climatic gradients (Fig. 1) allowing the discrimination of mean annual temperature and precipitation effects. Temperature and precipitation of the first climatic gradient ranged from 18.6 to 25.5�C and from 1359 and 1508mm. Along the second gradient, temperature and precipitation ranged from 17.7 to 26.8�C and from 1327 to 1817mm. Along the third gradient, temperature and precipitation ranged from 16.4 to 22�C and from 779 to 1491mm. Mean annual temperature and rainfall were extracted from the WORLDCLIM database (Hijmans et al., 2005), obtained from 1950 to 2000, on the basis of each geographic coordinates of sampling. At each plot, six soil cores, 0-10 cm depth, were sampled with a metallic cylinder. One of them was used to measure the bulk density and other physicochemical analyses. The other 5 were pooled, sieved at 2 mm, and coarse plant debris were removed. Composite samples were kept at 4�C prior to further fresh soil incubations and analyses. For ten plots along the three gradients sampling was triplicated in order to assess the soil intrinsic variability under similar climate parameters.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-01-09T17:03:21Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2017-02-24T14:45:20Z
attributes.tag: External Id
attributes.value: SAMEA100444918
attributes.tag: INSDC center name
attributes.value: INRA-CMSE
attributes.tag: INSDC first public
attributes.value: 2019-01-09T17:03:21Z
attributes.tag: INSDC last update
attributes.value: 2017-02-24T14:45:20Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: MadaTR_2014_RN7_95_16S
attributes.tag: collection date
attributes.value: 2014-05
attributes.tag: environment (biome)
attributes.value: terrestrial biome
attributes.tag: environment (feature)
attributes.value: anthropogenic geographic feature
attributes.tag: environment (material)
attributes.value: soil
attributes.tag: geographic location (country and/or sea)
attributes.value: Madagascar
attributes.tag: geographic location (depth)
attributes.value: 0,2
attributes.tag: geographic location (elevation)
attributes.value: 1443
attributes.tag: geographic location (latitude)
attributes.value: 47,13705
attributes.tag: geographic location (longitude)
attributes.value: -20,893894
attributes.tag: investigation type
attributes.value: metagenome
attributes.tag: multiplex identifiers
attributes.value: CGTCTAGTAC
attributes.tag: nucleic acid extraction
attributes.value: DNA was extracted using GnS-GII protocol as described in Terrat et al , 2012
attributes.tag: pcr primers
attributes.value: F479 (5-CAGCMGCYGCNGTAANAC-3) R888 (5-CCGYCAATTCMTTTRAGT-3)
attributes.tag: project name
attributes.value: CAMMiSolE_Grad_Clim_Mada
attributes.tag: sample name
attributes.value: MadaTR_2014_RN7_95_16S
attributes.tag: sample volume or weight for DNA extraction
attributes.value: 2
attributes.tag: scientific_name
attributes.value: soil metagenome
attributes.tag: sequencing method
attributes.value: 454-pyrosequencing
attributes.tag: soil environmental package
attributes.value: soil
attributes.tag: target gene
attributes.value: 16S rRNA gene