title: Single retinal ganglion cell OFF T alpha 4 from Mus musculus musculus
organism: Mus musculus musculus
description: Retinal ganglion cells were manually isolated based upon their physiological distinction through cell-attached recordings. Single cell RNA-Sequencing was employed for the characterization of transcriptomes to identify gene candidates that were expressed solely in ganglion cell subtypes. Design: Retinas from C57BL/6 mice were dissected under IR light (940 nm), various visual stimuli were projected upon the retina and the response of each RGC was monitored. Once a given cell was classified, a fire-polished glass electrode was used to aspirate the soma and transfer it to a nuclease-free PCR tube for processing. Single cell libraries were generated using SMART-seq protocols. The resulting samples were pooled and sequenced on an Illumina 2500, with an average read length of 100bp at the Iowa State University DNA Facility.
attributes.tag: strain
attributes.value: C57BL/6J
attributes.tag: age
attributes.value: Adult
attributes.tag: sex
attributes.value: not collected
attributes.tag: tissue
attributes.value: retina
attributes.tag: genotype
attributes.value: Wild type
attributes.tag: sub_species
attributes.value: musculus
attributes.tag: BioSampleModel
attributes.value: Model organism or animal