title: Sample 13
organism: Populus tremula x Populus alba
description: Protocols: Developing xylem and mature xylem were collected separately during the harvest and shortly frozen in liquid nitrogen. Plants were multiplied by in vitro propagation and kept on 1/2 MS-medium for four weeks. Afterwards, plants were grown in Long Ashton nutrient solution for 8 weeks until the treatment started. Poplars were kept in a culture room (air temperature: 24 °C, relative humidity: 25%, light regime: 16 h light/8 h dark, photosynthetic active radiation (PAR): 150 μmol photons m-2 s-1). Three treatments were applied for 30 days: control treatment (-ABA), continuous ABA treatment (+ABA), and discontinuous ABA treatment (±ABA). No ABA was fed to control plants. ABA was added to the Long Ashton solution to achieve the specified concentration. ABA (100 µM) was fed to +ABA plants for all 30 days of the treatment. For ±ABA plants, ABA was fed only at the beginning (0 to 2 days), the middle (14 to 16 days) and the end (28 to 30 days) of the treatment. Nucleic acid was extracted according to CTAB extraction protocol (Chang et al., 1993). TruSeq mRNA Sample Prep kit v2 (Illumina, San Diego, CA, USA) according to manufacturer's instructions.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-01-20T17:02:15Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-01-10T16:23:06Z
attributes.tag: External Id
attributes.value: SAMEA5209665
attributes.tag: INSDC center name
attributes.value: Georg-August-Universitat Busgen-Institut Department of Forest Botany and Tree Physiology
attributes.tag: INSDC first public
attributes.value: 2019-01-20T17:02:15Z
attributes.tag: INSDC last update
attributes.value: 2019-01-10T16:23:06Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7587:Sample 13
attributes.tag: age
attributes.value: 16
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cultivar
attributes.value: INRA717 1-B4
attributes.tag: developmental stage
attributes.value: sapling
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: xylem
attributes.tag: sample name
attributes.value: E-MTAB-7587:Sample 13
attributes.tag: sampling site
attributes.value: mature xylem
attributes.tag: scientific_name
attributes.value: Populus tremula x Populus alba
attributes.tag: stimulus
attributes.value: continuous abscisic acid