organism: Mus musculus
description: Protocols: 3T3-L1 or IBA cells were induced to differentiation at day 0 (2 days post-confluence) by adding the induction medium, which is complete culture medium supplemented with the MDI cocktail (1μM dexameth¬asone, 0.5 mM 3-isobutyl-1-methylxanthine, and 167 nM insulin, all from Sigma, Saint-Louis, MO). At day 2, induction medium was removed and maintainance medium (complete culture medium supplemented with 167 nM insulin) was added. At day 4, the maintenance medium was removed and complete culture medium was added. The cells were harvested for staining or RNA extraction at day 6. RNA extractions were performed using the Direct-zol RNA Miniprep kit (R2052, Zymo Research). Potential genomic DNA contamination was eliminated by Dnase I digestion. The RNA quality and concentration was checked by Nanodrop spectrophotometer (Thermo Scientific). RNA extractions were performed using the Direct-zol RNA Miniprep kit (R2052, Zymo Research). Potential genomic DNA contamination was eliminated by Dnase I digestion. The RNA quality and concentration was checked by Nanodrop spectrophotometer (Thermo Scientific).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-08-01T17:02:02Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-07-09T13:48:51Z
attributes.tag: External Id
attributes.value: SAMEA4780208
attributes.tag: INSDC center name
attributes.value: EPFL SV IBI-SV UPDEPLA
attributes.tag: INSDC first public
attributes.value: 2018-08-01T17:02:02Z
attributes.tag: INSDC last update
attributes.value: 2018-07-09T13:48:51Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6995:Ctrl_D0_1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: 3T3-L1
attributes.tag: cell type
attributes.value: fibroblast
attributes.tag: common name
attributes.value: house mouse
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: scrambled shRNA
attributes.tag: growth condition
attributes.value: adipogenic differentiation
attributes.tag: organism part
attributes.value: embryo
attributes.tag: replicate
attributes.value: 1
attributes.tag: sample name
attributes.value: E-MTAB-6995:Ctrl_D0_1
attributes.tag: scientific_name
attributes.value: Mus musculus