organism: Trifolium pratense
description: Trifolium pratense ''Marathon'' seeds were planted in Leonard jars containing vermiculite and perlite (3 to 1 mixture) and inoculated with Rhizobium leguminosarum bv. trifolii (isolated from Nitragin Gold Clover pre-inoculant powder) at a rate of 10^8 cells per seedling. Plants were watered with 0.25X Hoagland''s nitrogen-free nutrient solution and grown in a growth chamber at 22C and with a 16 h photoperiod. Nodules were harvested 30 days post inoculation, and and flash frozen in liquid nitrogen. RNA was extracted from nodules, using an RNeasy Plant Mini Kit.
attributes.tag: cultivar
attributes.value: Marathon
attributes.tag: age
attributes.value: 30 days post inoculation
attributes.tag: geo_loc_name
attributes.value: USA: Minnesota, growth chamber
attributes.tag: tissue
attributes.value: Nodules
attributes.tag: biomaterial_provider
attributes.value: Nevin Young
attributes.tag: collected_by
attributes.value: Diana Trujillo
attributes.tag: BioSampleModel
attributes.value: Plant