organism: Mus musculus
description: sequencing of miRNA from wild type and diseased mouse hearts using ligation-based bar-codes. Samples of cardiac left ventricle were taken from three wild-type mouse and a pooled sample was created. Samples of cardiac left ventricle were also taken from three diseased mouse (Hypertrophic cardiomyopathy) and a pooled sample was created. miRNA were extracted from these two samples using the AMbion mirVana miRNA Isolation Kit (Ambion AM1561). The two samples were prepared in parallel; each mouse miRNA sample was split into ten equal aliquots, and marked by the same set of ten different 5’ ligation bar-codes during the library preparation. The two sets of ten libraries were sequenced by Illumina's GAIIx instrument in a single flow cell, each set in a single laneillumina GAIIx instrument.