organism: synthetic construct
description: 14 type IIS restriction enzymes (AcuI, BbvI, BpmI, BpuEI, BseRI, BsgI, BsmFI, Eco57I, Eco57MI, FauI, FokI, GsuI, MmeI and SmuI) and one type III restriction enzyme (EcoP15I) were assayed for their variation in cleavage distance. For each enzyme an oligonucleotide was ordered that included the recognition sequence and a randomer spanning the distance between the recognition site and the cut site. These oligos were treated with corresponding restriction enzyme pooled and sequenced. 
attributes.tag: strain
attributes.value: missing
attributes.tag: collection_date
attributes.value: missing
attributes.tag: geo_loc_name
attributes.value: missing
attributes.tag: isolation_source
attributes.value: missing
attributes.tag: sample_type
attributes.value: missing
attributes.tag: BioSampleModel
attributes.value: Microbe, viral or environmental