organism: Sus scrofa
description: Protocols: The uterus was flushed with 10 ml and another 50 ml of phosphate-buffered saline (autoclaved, ph 7.4). Then, endometrial samples were collected, shock frozen in liquid nitrogen and stored at - 80 °C. We analyzed the effects of gestational estradiol-17β (E2) exposure on the endometrium. Two distinct low doses corresponding to the acceptable daily intake (ADI) and close to the no-observed effect level (NOEL) and a high dose (0.05, 10 and 1000 µg E2/kg body weight daily, respectively) were orally applied to sows from insemination until sampling at day 10 of pregnancy and compared to carrier-treated controls. RNA was harvested by means of TRIzol reagent (n = 4 per group, except for the ADI group, where a repeated tissue extraction was done because of only n=3 available animals). The library preparation starting from 125 ng total RNA of each endometrial sample was performed with the Encore Complete RNA-Seq Multiplex System IB (NuGEN, AC Leek, The Netherlands) following the manufacturers' protocol.
attributes.tag: ENA first public
attributes.value: 2018-10-16
attributes.tag: ENA last update
attributes.value: 2017-11-29
attributes.tag: External Id
attributes.value: SAMEA104408997
attributes.tag: INSDC center alias
attributes.value: ETH Zurich, Animal Physiology, Institute of Agricultural Sciences, Zurich, Switzerland
attributes.tag: INSDC center name
attributes.value: ETH Zurich, Animal Physiology, Institute of Agricultural Sciences, Zurich, Switzerland
attributes.tag: INSDC first public
attributes.value: 2018-10-16T17:02:44Z
attributes.tag: INSDC last update
attributes.value: 2017-11-29T14:54:36Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6242:Sample 16
attributes.tag: breed
attributes.value: German Landrace
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: pig
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: endometrium
attributes.tag: sample name
attributes.value: E-MTAB-6242:Sample 16
attributes.tag: sampling time point
attributes.value: day 10 of pregnancy
attributes.tag: sex
attributes.value: female