organism: Mercurialis annua
description: Protocols: Collect of mature leafs from ten individuals randomly taken from a particular experimental line (diploids) or natural population (tetraploids). Plant growth in soil with 2 g of slow-release fertilizer per litre of soil in controlled greenhouse conditions Control lines were let to evolve during four generations in populations with 1:1 sex ratio. Selected lines are one females let to evolve in male-deprived population, reproducing only through inconstancy of sex expression Pools of tissues from 10 individuals. Total RNA extraction using Maxwell 16 Instrument IVD processor Nucleic acid and protein purification (Promega) following manufacturer recommandations Illumina stranded TruSeq RNA library incl. poly(A) enrichment
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-08-01T17:02:01Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-06-14T12:17:40Z
attributes.tag: External Id
attributes.value: SAMEA4729065
attributes.tag: INSDC center name
attributes.value: Department of Ecology and Evolution University of Lausanne
attributes.tag: INSDC first public
attributes.value: 2018-08-01T17:02:01Z
attributes.tag: INSDC last update
attributes.value: 2018-06-14T12:17:40Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6905_2:L3F
attributes.tag: age
attributes.value: 7
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cultivar
attributes.value: European diploid
attributes.tag: developmental stage
attributes.value: sexually mature individuals
attributes.tag: genotype
attributes.value: selected for increased male allocation
attributes.tag: individual
attributes.value: selected line 3
attributes.tag: organism part
attributes.value: mature leaf
attributes.tag: sample name
attributes.value: E-MTAB-6905_2:L3F
attributes.tag: scientific_name
attributes.value: Mercurialis annua
attributes.tag: sex
attributes.value: canalized female