organism: Mus musculus
description: Protocols: After sacrifice, the blood was flushed from circulation by infusing pressurized phosphate buffered saline from the left ventricle of the animal. The entire aortae, which included the distal portion of the ascending aortae, aortic arches, and descending aortae down to the liac bifurcations, were excised from the sacrificed animals, under dissecting microscope. The excised aortae were thoroughly cleaned under dissection microscope to be free of other tissue such as adipose, muscle, and connective tissues. They were then stored in -80 degree until the extraction of RNA. After sacrifice, the blood was flushed from circulation by infusing pressurized phosphate buffered saline from the left ventricle of the animal. The entire aortae, which included the distal portion of the ascending aortae, aortic arches, and descending aortae down to the liac bifurcations, were excised from the sacrificed animals, under dissecting microscope. The excised aortae were thoroughly cleaned under dissection microscope to be free of other tissue such as adipose, muscle, and connective tissues. They were then stored in -80 degree until the extraction of RNA. All mice were placed on high-fat diet (HFD, 1.25% cholesterol, 40% kcal fat; D12108Ci, Research Diets, Inc., New Brunswick, NJ) at 8th week of age and maintained on the diet for 16 weeks. HFD contained either ticagrelor, clopidogrel, or nothing (control). The mice were fed HFD containing either ticagrelor (180mg/kg/day), clopidogrel (25 mg/kg/day), or nothing (control). The doses of ticagrelor and clopidogrel completely inhibited the platelet aggregation by light transmission aggregometry. Illumina software bcl2fastq v2.17.1.14 was used to convert bcl files into fastq format. Total RNA was isolated individually from each aorta using Qiagen RNA purification columns. The quality of the RNA was examined, using the Agilent 2100 Bioanalyzer and found that all samples had RNA integrity numbers higher than 8. RNA samples were polyA selected and paired-end sequencing libraries were constructed using TruSeq RNA Sample Prep Kit as described in the TruSeq RNA Sample Preparation V2 Guide (Illumina).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-06-16T04:04:29Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-06-11T09:51:34Z
attributes.tag: External Id
attributes.value: SAMEA5704570
attributes.tag: INSDC center name
attributes.value: University of Texas Medical Branch at Galveston (UTMB)
attributes.tag: INSDC first public
attributes.value: 2019-06-16T04:04:29Z
attributes.tag: INSDC last update
attributes.value: 2019-06-11T09:51:34Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8050:ticagrelor in high-fat-diet #2
attributes.tag: age
attributes.value: 8
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: LDLR-/-APOBEC1-/-
attributes.tag: organism part
attributes.value: aorta
attributes.tag: sample name
attributes.value: E-MTAB-8050:ticagrelor in high-fat-diet #2
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: C57BL/6J