organism: Homo sapiens
description: Protocols: Use of tissue samples remaining after diagnosis for scientific reasons was approved by Medical Ethical Committee (MEC) of the Erasmus MC Rotterdam (The Netherlands), permission 02.981. This included the permission to use the secondary tissue without further consent. Samples were used according to the 'Code for Proper Secondary Use of Human Tissue in The Netherlands' developed by the Dutch Federation of Medical Scientific Societies (FMWV (Version 2002, update 2011)). Tissues were stored frozen in liquid nitrogen or as formalin-fixed and paraffin-embedded (FFPE) blocs. RNA was extracted from frozen tissue samples using TRIZOL and purified on RNeasy column (Qiagen) QC was performed on Agilent Bioanalzer using a Eukaryote total RNA Pico chip, vs 2.6. RNA samples were rRNA reduced with RiboZero Gold and library preparation was performed using the using the Ion Total RNA-seq kit
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-07-31T17:02:12Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2017-05-16T18:32:05Z
attributes.tag: External Id
attributes.value: SAMEA104062033
attributes.tag: INSDC center name
attributes.value: unspecified
attributes.tag: INSDC first public
attributes.value: 2018-07-31T17:02:12Z
attributes.tag: INSDC last update
attributes.value: 2017-05-16T18:32:05Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-5746:T6107-NS
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: clinical history
attributes.value: Chemotherapy-resistant cancer, after orchiectomy
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: testicular non-seminomatous germ cell tumor
attributes.tag: individual
attributes.value: T6107
attributes.tag: organism part
attributes.value: testis
attributes.tag: sample name
attributes.value: E-MTAB-5746:T6107-NS
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: male
attributes.tag: specimen with known storage state
attributes.value: frozen tissue sample