organism: Mus musculus
description: Protocols: Tissues were collected from timed matings with the day of the copulation plug as E0.5. Embryos were dissected into hindbrain and heart. Indexed DNA libraries were obtained by end repair, A-tailing and adapter ligation (NEBNext Ultra II DNA library Prep kit) following the manufacturer's guideline. Per indexed library, cytosine or fully modified 5mC, 5hmC or 5fC DNA was subsequently generated by PCR (25 cycles) using the Taq polymerase and dCtp, dmCtp, dhmCtp and dfCtp and purified by GeneJET PCR Purification Kit and eluted with ultra pure water to generate gDNA_C, gDNA_mC, gDNA_hmC and gDNA_fC input samples.
attributes.tag: ENA first public
attributes.value: 2018-10-22
attributes.tag: ENA last update
attributes.value: 2017-12-07
attributes.tag: External Id
attributes.value: SAMEA104420734
attributes.tag: INSDC center alias
attributes.value: Senior research associate
attributes.tag: INSDC center name
attributes.value: Senior research associate
attributes.tag: INSDC first public
attributes.value: 2018-10-22T17:03:09Z
attributes.tag: INSDC last update
attributes.value: 2017-12-07T13:57:46Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6271:gDNA_hmC_input
attributes.tag: age
attributes.value: embryonic day 11.5
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: hindbrain
attributes.tag: sample name
attributes.value: E-MTAB-6271:gDNA_hmC_input
attributes.tag: sex
attributes.value: mixed