organism: bovine gut metagenome
description: High molecular weight DNA extracted from the post-incubated switchgrass adherent rumen microbiome was used as a template for three different sequencing libraries. Two whole genome shotgun sequencing libraries with insert size 200 bp and 300 bp were independently generated from DNA, followed by high-throughput sequencing from both ends, one by 2x125bp using Illumina GAIIx and one by 2x100bp using Illumina HiSeq2000 instruments. Two additional libraries were built with insert sizes of 3kb and 5kb respectively to facilitate genome assembly. Both of these two “jumping” libraries were sequenced using Illumina GAIIx by 2x75bp pair-end sequencing.