organism: Mus musculus
description: Protocols: The ChIP procedure and analysis were carried out essentially as described previously (PMID: 27773674). Briefly, mESCs were grown in 2iL medium. The cells were detached, and fixed by 1.5% formaldehyde in PBS for 15min. Chromatin was solubilized by sonication, and sheared to < 500 bp fragments. Dazl immunoprecipitation was carried out using CST antibodies #8042 overnight at 4 °C. Following washing steps, chromatin was eluted, and DNA was purified The eluted chromatin was treated with proteinase K (20ug) to degrade proteins. Then the samples were heated at 95 C for 10 min to reverse the crosslinking. Then DNA was purified by SPRI magnetic beads 2x vol of the samples. After washing with 80% ethanol, DNA was eluted in Tris HCl 10mM. DNA library was prepared using NEBNext Illumina Ultra II components. The libraries were indexed using NEBNext Multiplex Oligos. The size distribution of the libraries were checked using TapeStation, and the libraries were pooled.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-12-25T04:05:25Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-07-03T21:36:16Z
attributes.tag: External Id
attributes.value: SAMEA7039542
attributes.tag: INSDC center name
attributes.value: Postdoc researcher The Francis Crick institute
attributes.tag: INSDC first public
attributes.value: 2020-12-25T04:05:25Z
attributes.tag: INSDC last update
attributes.value: 2020-07-03T21:36:16Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-9302:Dazl_R2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: embryonic stem cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: sample name
attributes.value: E-MTAB-9302:Dazl_R2
attributes.tag: scientific_name
attributes.value: Mus musculus