organism: Mus musculus
description: Protocols: TDG KO mice contain a deletion of the coding sequence for the catalytic domain of TDG, which is replaced by a neomycin-resistant cassette. Genotyping was carried out using DNA isolated from the yolk sac as template with the following primers:TDG KO/WT forward: 5′-GGGCAGCAAGGATCTGTCTA-3′TDG KO/WT reverse: 5′-GCCAGTTCCTCTGACACTTAGC-3′Mice were maintained as heterozygotes and intercrossed to generate homozygous knockout embryos for analysis. Tissues were collected from timed matings with the day of the copulation plug as E0.5. Embryos were dissected into hindbrain and heart. Tissues were collected from timed matings with the day of the copulation plug as E0.5. Embryos were dissected into hindbrain and heart. Total RNA for each sample was extracted using RNeasy mini kit (QIAGEN) and following the manufacturer's instructions. For RNA-seq, total RNA for each sample was extracted using RNeasy mini kit (QIAGEN) and following the manufacturer's instructions. Extracted RNA was polyA-enriched. Then, RNA was used for library preparation using the TruSeq RNA Library Prep Kit v2 (Illumina) following the manufacturer's instructions.
attributes.tag: ENA first public
attributes.value: 2018-10-22
attributes.tag: ENA last update
attributes.value: 2017-12-07
attributes.tag: External Id
attributes.value: SAMEA104420761
attributes.tag: INSDC center alias
attributes.value: Senior research associate
attributes.tag: INSDC center name
attributes.value: Senior research associate
attributes.tag: INSDC first public
attributes.value: 2018-10-22T17:03:09Z
attributes.tag: INSDC last update
attributes.value: 2017-12-07T13:57:47Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6271:rnaseq_brain_KO_2
attributes.tag: age
attributes.value: embryonic day 11.5
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: genotype
attributes.value: Tdg knockout
attributes.tag: organism part
attributes.value: hindbrain
attributes.tag: sample name
attributes.value: E-MTAB-6271:rnaseq_brain_KO_2
attributes.tag: sex
attributes.value: mixed