organism: Danio rerio
description: Protocols: The ChIP assay was carried out within the tgf-β mutant and sibling embryos at 32 hpf in zebrafish. The sonicated DNA was precipitated by Jun or Normal Rabbit IgG antibodies. Afterward, the precipitated DNA was collected by QIAquick PCR Purification Kit. The gene binding profiles were detected by deep-sequencing. DNA samples were end-repaired, A tailed, and adaptor ligated using TruSeq Nano DNA Sample Prep Kit (#FC-121-4002, Illumina), following the manufacturer's instructions. 200 - 500 bp fragments were selected at the gel size fractionation step. The final size of the library was confirmed by gel electrophoresis.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2017-06-08T17:01:19Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2017-05-16T18:32:16Z
attributes.tag: External Id
attributes.value: SAMEA104016676
attributes.tag: INSDC center name
attributes.value: unspecified
attributes.tag: INSDC first public
attributes.value: 2017-06-08T17:01:19Z
attributes.tag: INSDC last update
attributes.value: 2017-05-16T18:32:16Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-5699:Mu_Jun_2
attributes.tag: age
attributes.value: 32
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: zebrafish
attributes.tag: developmental stage
attributes.value: embryo
attributes.tag: genotype
attributes.value: tgfb mutant
attributes.tag: sample name
attributes.value: E-MTAB-5699:Mu_Jun_2
attributes.tag: scientific_name
attributes.value: Danio rerio