organism: Mus musculus
description: Protocols: Splenic B cells were collected from TG2-sufficient and TG2-deficient mouse under resting and stimulated (2 µg / ml murine TG2 for 120 min) conditions. Total RNA was isolated from B cells with an RNeasy minikit (Qiagen). RNA integrity was analyzed on Agilent Bioanalyzer using Agilent RNA 6000 Pico kit. RNA integrity was 9.1 ± 0.7. RNA-seq libraries were generated from purified rRNA-depleted RNA samples using the strand-specific TruSeq stranded mRNA library prep kit (Illumina, San Diego, CA, USA).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-11-08T04:04:44Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-11-07T12:37:46Z
attributes.tag: External Id
attributes.value: SAMEA6162969
attributes.tag: INSDC center name
attributes.value: K. G Jebsen centre for Coeliac disease research, Department of Immunology, Institute of Clinical Medicine, University of Oslo
attributes.tag: INSDC first public
attributes.value: 2019-11-08T04:04:44Z
attributes.tag: INSDC last update
attributes.value: 2019-11-07T12:37:46Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7567:Sample 21
attributes.tag: age
attributes.value: 9
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: B cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: genotype
attributes.value: 14E06 Ig KI HLA-DQ2.5 tg
attributes.tag: individual
attributes.value: 606
attributes.tag: organism part
attributes.value: spleen
attributes.tag: phenotype
attributes.value: Tgm2+
attributes.tag: sample name
attributes.value: E-MTAB-7567:Sample 21
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: C57BL/6