organism: Mus musculus
description: Protocols: Mouse embryonic stem cells were UV cross linked and lysed in 50 mM TrisвЂ“HCl, pH 7.4, 100 mM NaCl, 1% Igepal CA-630, 0.1% SDS, 0.5% sodium deoxycholate and ROCHE c0mplete protease inhibitor cocktail (1x) RNA cross-linked to DAZL was immunoprecipitated and PAGE was performed on protein-RNA complexes. After transfer of the protein-RNA complexes to nitrocellulose (wet blotting) the complexes were digested with Proteinase K and the liberated RNA-peptide complexes purified using PCA. Library construction was carried out according to the iCLIP protocol described elsewhere [1]. RNA was ligated with a IRL3 3'adapter, circularised and reverse transcribed using barcoded RT primers. [1] Blazquez L et al. Mol Cell 2018. doi: 10.1016/j.molcel.2018.09.033
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-12-25T04:05:25Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-07-09T15:56:48Z
attributes.tag: External Id
attributes.value: SAMEA7050870
attributes.tag: INSDC center name
attributes.value: The Francis Crick Institute
attributes.tag: INSDC first public
attributes.value: 2020-12-25T04:05:25Z
attributes.tag: INSDC last update
attributes.value: 2020-07-09T15:56:48Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-9332:Dazl iCLIP
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: 46C
attributes.tag: cell type
attributes.value: embryonic stem cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: embryo
attributes.tag: disease
attributes.value: normal
attributes.tag: sample name
attributes.value: E-MTAB-9332:Dazl iCLIP
attributes.tag: scientific_name
attributes.value: Mus musculus