organism: Bos taurus
description: Protocols: Oocytes were collected from cows using transvaginal ultrasound-guided ovum pick-up (OPU). Using an ultrasound probe system (General Electric) with a cannula connected to an aspiration pump (Minitube), subordinate follicles of size smaller than 8mm were aspirated. Oocytes were mechanically denuded using hyaluronidase and snap frozen. Pooled oocytes were lysed and bisulfite treated using the EZ-DNA Methylation-Direct Kit (Zymo Research) following manufacturer's instructions. Sequencing libraries were generated from the bisulfite treated DNA using the Pico Methyl-Seq Library Prep Kit (Zymo) according to manufacturer's recommendations with 20µM PrepAmp primer mix and 10-cycle PCR amplification.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-07-23T04:03:27Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-07-26T16:53:37Z
attributes.tag: External Id
attributes.value: SAMEA5816282
attributes.tag: INSDC center name
attributes.value: Leibniz-Institute for Farm Animal Biology (FBN) Institute of Genome Biology/ Genomics Unit
attributes.tag: INSDC first public
attributes.value: 2020-07-23T04:03:27Z
attributes.tag: INSDC last update
attributes.value: 2019-07-26T16:53:37Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8191:ER1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: cattle
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: organism part
attributes.value: oocytes
attributes.tag: sample name
attributes.value: E-MTAB-8191:ER1
attributes.tag: scientific_name
attributes.value: Bos taurus
attributes.tag: sex
attributes.value: female
attributes.tag: study group
attributes.value: 5-6 week post-partum cows
attributes.tag: breed
attributes.value: Holstein-Friesian