organism: Solanum lycopersicum
description: Protocols: Samples were harvested at the end of the day, and consisted of pools of at least a hundred whole shoot apical meristems or at least six first leaves. Samples were collected from two week old plants of tomato. S. lycopersicum seeds were directly sown in soil (Levington F2, ICL) and stratified in the dark for one week at 4°C. Plants were grown in a Conviron MTPS chamber (Conviron) at 25/18°C (day/night); with 16/8 hours light cycle, 300 µmol/m2/s light intensity and ~60% humidity. DNA was extracted with the DNeasy Plant Mini Kit (Qiagen). PCR-free libraries for the DNA-seq were prepared with 1 ug of DNA using the TruSeq DNA PCR-Free Library Prep kit (Illumina); following provider's instructions.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-06-14T04:02:46Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-03-29T14:10:02Z
attributes.tag: External Id
attributes.value: SAMEA5542008
attributes.tag: INSDC center name
attributes.value: IFEVA (CONICET-UBA), Facultad de Agronomia (FAUBA),Universidad de Buenos Aires (UBA)
attributes.tag: INSDC first public
attributes.value: 2019-06-14T04:02:46Z
attributes.tag: INSDC last update
attributes.value: 2019-03-29T14:10:02Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7823:Mersitems_A_lane1
attributes.tag: age
attributes.value: 15 days old
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: tomato
attributes.tag: cultivar
attributes.value: M82
attributes.tag: developmental stage
attributes.value: vegetative
attributes.tag: genotype
attributes.value: wild-type
attributes.tag: organism part
attributes.value: shoot apical meristem
attributes.tag: sample name
attributes.value: E-MTAB-7823:Mersitems_A_lane1
attributes.tag: scientific_name
attributes.value: Solanum lycopersicum