organism: freshwater metagenome
description: Microbial community of Bolshaya Osinovka river mouth
attributes.tag: isolation_source
attributes.value: water
attributes.tag: collection_date
attributes.value: 2018-03-21
attributes.tag: geo_loc_name
attributes.value: Russia:Lake Baikal
attributes.tag: lat_lon
attributes.value: 51.50056 N 104.24403 E
attributes.tag: samp_collect_device
attributes.value: Shale Niskin
attributes.tag: samp_mat_process
attributes.value: Water samples (5 L) were filtered on nitrocellulose filters (25 mm diameter, 0.2 micron pore size; Millipore, Germany) using a peristaltic pump. The filter was then placed in a TE buffer (10 mM Tris-HCl, pH 7.4; 1 mM EDTA, pH 8.0) and frozen at -20. Then, it was transported to the laboratory. DNA was extracted according to the modified method of phenol-chloroform extraction (Sambrook et al. 1989). The extracted DNA was kept at -70C for the further use.
attributes.tag: samp_size
attributes.value: 5L
attributes.tag: BioSampleModel
attributes.value: Metagenome or environmental