organism: Drosophila melanogaster
attributes.tag: isolate
attributes.value: Control C1-F1-75 of SAIP from DGRP
attributes.tag: age
attributes.value: 11-14 days
attributes.tag: dev_stage
attributes.value: adult
attributes.tag: sex
attributes.value: female
attributes.tag: tissue
attributes.value: whole body
attributes.tag: biomaterial_provider
attributes.value: Yazmin L. Serrano Negron
attributes.tag: birth_location
attributes.value: National Heart Lung and Blood Institute
attributes.tag: breeding_history
attributes.value: We created the Sleep Advanced Intercross Population (SAIP) from the 5 longest-sleeping and 5 shortest-sleeping lines of the Drosophila Genetic Reference Panel (DGRP). The lines we used were DGRP-235, DGRP-313, DGRP-335, DGRP-338, and DGRP-379 (long sleepers); and DGRP-38, DGRP-310, DGRP-365, DGRP-808, and DGRP-832 (short sleepers). These lines are maximally homozygous; the genome-wide heterozygosity is less than 5%. DGRP-38 is heterozygous for the In(3R)K inversion; DGRP-313 is homozygous for the In(2L)t inversion; DGRP-335 is heterozygous for the In(3R)Mo inversion; DGRP-338 is heterozygous for the In(2L)t and In(2R)NS inversions; and the remaining lines have the standard karyotype for all common polymorphic inversions. DGRP-310, DGRP-335, DGRP-338, DGRP-365, and DGRP-832 were infected with Wolbachia pipientis; the remaining lines were not. All ten lines were crossed in a full diallel design, resulting in 100 crosses. Two virgin females and two males from the F1 of each cross were randomly mixed together and placed into 20 bottles, with 10 males and 10 females in each bottle. At each generation thereafter, 20 virgin females and 20 males from each bottle were randomly mixed with the other flies, and a new generation of 20 bottles was reared. Each generation of random mating had a census population size of 800. The genomes of these flies were allowed to recombine in this manner for 21 generations, resulting in an outbred population of flies.
attributes.tag: breeding_method
attributes.value: We began with the SAIP base population derived from the 5 longest-sleeping and 5 shortest-sleeping lines of the DGRP. We measured sleep in 100 virgin females and 100 virgin males, and chose 25 individuals of each sex at random to establish control populations. Each subsequent generation, we chose 25 virgin males and 25 virgin females at random from 100 measured of each sex as parents of the next generation. We continued this process for 75 generations.
attributes.tag: collected_by
attributes.value: Laboratory of Systems Genetics
attributes.tag: geo_loc_name
attributes.value: USA: Bethesda, MD
attributes.tag: phenotype
attributes.value: Sleep duration
attributes.tag: sample_type
attributes.value: whole organism
attributes.tag: treatment
attributes.value: None
attributes.tag: BioSampleModel
attributes.value: Model organism or animal