organism: Arabidopsis thaliana
description: Protocols: Unopened flower buds at floral stage 9 were collected in tubes, snap frozen in liquid nitrogen and stored at -80°C until further use. Plants were grown under long day conditions (16 hours light/8 hours dark) at 20°C. 6 μg of total RNA was treated with DNAseI using TURBO DNase reagents (ThermoFisher, AM2238). 4 μg of total DNAseI-treated total RNA was then treated with Ribo-Zero rRNA removal magnetic kit (Epicentre, MRZPL116) to deplete rRNA from the total RNA sample. 100 mg of unopened flower buds were ground in liquid nitrogen and RNA was extracted using Trizol reagent. 50 ng of rRNA-depleted DNAseI-treated RNA was used to prepare a stranded RNA-seq library using the ScriptSeq v2 RNA-seq Library Preparation Kit (Epicentre, SSV21124), as described in Choi et al. (2018) Genome Res. 28(4): 532-546.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-11-10T04:03:12Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-11-01T14:13:45Z
attributes.tag: External Id
attributes.value: SAMEA5059739
attributes.tag: INSDC center name
attributes.value: Department of Plant Sciences, University of Cambridge
attributes.tag: INSDC first public
attributes.value: 2019-11-10T04:03:12Z
attributes.tag: INSDC last update
attributes.value: 2018-11-01T14:13:45Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7371:wt_RNAseq_Rep1
attributes.tag: age
attributes.value: 6
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: thale cress
attributes.tag: developmental stage
attributes.value: floral organ formation stage
attributes.tag: ecotype
attributes.value: Col-0
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: growth condition
attributes.value: long day length regimen
attributes.tag: organism part
attributes.value: flower bud
attributes.tag: sample name
attributes.value: E-MTAB-7371:wt_RNAseq_Rep1
attributes.tag: scientific_name
attributes.value: Arabidopsis thaliana