organism: mouse gut metagenome
description: We investigated whether dietary iron alters microbiota composition. To ensure a uniform and normalized microbiota composition, we first cohoused 4-6-week-old mice by feeding standard chow diet for 14 days (weeks 1& 2). Their diet was switched to iron-enriched (350 ppm) diet for 14 days (weeks 3& 4). Then the mice were divided into two iron diet groups for the next 14 days (weeks 5& 6): group 1 was maintained on iron-enriched (350 ppm) diet and group 2 was fed with iron-deficient diet (<5-ppm). We examined the effect of dietary iron on the gut microbiota by collecting feces at the baseline (after completion of co-housing; at the end of week 2), at the completion iron enrichment by 350-ppm diet (at the end of week 4) and the end of the iron manipulation (at the end of week 6) followed by determining the microbiota composition by Illumina 16S rRNA gene sequencing.
attributes.tag: collection_date
attributes.value: 2019-06-19
attributes.tag: env_broad_scale
attributes.value: intestinal microbiome
attributes.tag: env_local_scale
attributes.value: duodenum, feces
attributes.tag: env_medium
attributes.value: intestinal contents
attributes.tag: geo_loc_name
attributes.value: USA: Ann Arbor
attributes.tag: host
attributes.value: Mus musculus
attributes.tag: lat_lon
attributes.value: 42.28 N 83.74 W
attributes.tag: isolation_source
attributes.value: duodenum, feces
attributes.tag: BioSampleModel
attributes.value: MIMS.me
attributes.tag: BioSampleModel
attributes.value: MIGS/MIMS/MIMARKS.host-associated