organism: Saccharomyces cerevisiae
description: Protocols: For the time course experiments, the indicated strains were grown in 450ml of SC-raffinose to mid-log phase (OD600 ~0.6) at 22℃. 50ml of cells were collected by centrifugation (raffinose 0). The remaining cells were also recovered by centrifugation, resuspended in SC media lacking any carbon source, and then transferred to media containing the indicated carbon sources. 50ml of cells were collected by centrifugation at the indicated time points. Total RNAs were isolated with hot acidic phenol/chloroform (1:1) (Sigma). Sequencing libraries were prepared using the TruSeq Stranded Total RNA Library Prep Kit (Illumina) after ribosomal RNA was depleted using the Ribo-Zero yeast kit (Epicenter), as recommended by manufacturers.
attributes.tag: ENA first public
attributes.value: 2018-10-23
attributes.tag: ENA last update
attributes.value: 2018-02-28
attributes.tag: External Id
attributes.value: SAMEA104655536
attributes.tag: INSDC center alias
attributes.value: Ewha Womans University
attributes.tag: INSDC center name
attributes.value: Ewha Womans University
attributes.tag: INSDC first public
attributes.value: 2018-10-23T17:03:39Z
attributes.tag: INSDC last update
attributes.value: 2018-02-28T15:11:40Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6551:pho23delta-Ra-0
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: baker's yeast
attributes.tag: genotype
attributes.value: pho23delta
attributes.tag: growth condition
attributes.value: raffinose
attributes.tag: sample name
attributes.value: E-MTAB-6551:pho23delta-Ra-0
attributes.tag: strain
attributes.value: YSB1319