organism: Homo sapiens
description: Protocols: For SPOP mutant over-expression a derivate of the pLX304 vector was used in which the CMV promoter has been exchanged to a PGK promoter and the blastocidin cassette exchanged by a puromycin resistance cassette (pLX_TRC_307, available at Addgene as Plasmid 41392, pCW107). All ORFs were cloned into pLX_TRC_307-mOrange using Nhe1 and Mlu1. VCaP were grown in DMEM (Gibco) and supplemented with 10% FBS (Gibco), 1% Penicilin/Streptomycin/L-Glutamate. All cells were incubated at 37°C and 5% CO2. Cells were routinely tested for mycoplasma contamination. DNA was extracted using the Quiagen kit and sample quality was assessed using Agilent Bioanalyzer. DNA libraries were prepared for sequencing using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®
attributes.tag: ENA first public
attributes.value: 2020-12-18
attributes.tag: ENA last update
attributes.value: 2020-12-17
attributes.tag: External Id
attributes.value: SAMEA7730616
attributes.tag: INSDC center alias
attributes.value: IOR - Institute of Oncology Research
attributes.tag: INSDC center name
attributes.value: IOR - Institute of Oncology Research
attributes.tag: INSDC first public
attributes.value: 2020-12-18T04:05:36Z
attributes.tag: INSDC last update
attributes.value: 2020-12-17T17:18:05Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-71741608225450:VCAP_CONTROL
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: VCaP
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: prostate carcinoma
attributes.tag: genotype
attributes.value: empty vector
attributes.tag: metastatic site
attributes.value: vertebra
attributes.tag: organism part
attributes.value: prostate gland
attributes.tag: sample name
attributes.value: E-MTAB-71741608225450:VCAP_CONTROL