organism: Homo sapiens
description: Protocols: T24 cells were obtained from American Type Culture Collection. To generate the cisplatin resistant cells, T24 were treated with a gradual increment (4, 8, 16, 32, 64 ug/ml cisplatin) with a discontinuous period until cells recover. 10^6 cells T24R and T24 cells were harvested for RNA-seq. 5 ug RNA was extracted by Trizol and treated with DNase I following the manufacturer's instructions. RNA quality was assessed using a Total RNA Nano chip with a 2100 Bioanalyzer instrument (Agilent). RNA-seq libraries were prepared from T24 cells using NEBNext Ultra II RNA library Prep Kit for Illumina (NEB,E7770S). Library quality was assessed using a DNA1000 chip with a 2100 Bioanalyzer instrument (Agilent).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-11-11T04:04:44Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-11-28T15:57:40Z
attributes.tag: External Id
attributes.value: SAMEA5139351
attributes.tag: INSDC center name
attributes.value: Department of urology, Taizhou people's hospital, Taizhou, Jiangsu, 225300, China.
attributes.tag: INSDC first public
attributes.value: 2019-11-11T04:04:44Z
attributes.tag: INSDC last update
attributes.value: 2018-11-28T15:57:40Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7437:Sample 2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: T24
attributes.tag: cell type
attributes.value: epithelial cell
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: Transitional Cell Carcinoma
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: urinary bladder
attributes.tag: sample name
attributes.value: E-MTAB-7437:Sample 2
attributes.tag: scientific_name
attributes.value: Homo sapiens