organism: Apis mellifera
description: Protocols: Bees were developing in a brood frame Varroa-free colony for 3 days after hatching from egg. Then entire the brood frame with bee larvae was transferred to a Varroa-infested colony with high levels of Deformed wing virus (DWV). Both Varroa-free colony and Varroa-infested colony were kept in outdoor flight cages, bees were fed with pollen sourced in Varroa-free region and with sugar syrup. Larvae of worker honeybee, Apis mellifra (day 3 of bee development), which were hatched from the eggs laid in a colony free from Varroa destructor mites and harbouring low levels of Deformed wing virus (DWV), were either left to develop in the same Varroa-free hive before sampling at pupal stage (day 15) (EnvironmentalHistory-NoTransfer), or were transferred to a Varroa-infested colony with high levels of DWV in bees and the mites (EnvironmentalHistory-TransferToVarroaColony). All transferred larvae were receiving the DWV strains present in this Varroa-infested colony with the food delivered by the nurse bees until capping on day 9. About half of these larvae were capped with Varroa mite and were subjected to the mite piercing and feeding on their haemolymph for 5 days during pupal development until sampling on day 14 (GrowthCondition -MiteFeeding), the rest of the pupae developed without the mite (GrowthCondition- NoMite). Exposure to the mite piercing and feeding resulted in about 1000-fold increase of the DWV levels in about 80% of the mite-exposed pupae (DiseaseState-HighDWV). In the rest of mite-exposed pupae the levels of DWV remained relatively low (DiseaseState-LowMidDWV) and were similar to those in the pupae not exposed to the mite. TRIzol reagent (Invitrogen) extraction of total RNA from individual whole pupae. Purification of the 15 to 40 nt RNA fraction using gel extraction. Small RNA (siRNA and miRNA) library cloning using Illumina TruSeq Small RNA Sample Preparation Kit according to manufactirers instructions
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2013-09-01T17:01:52Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T16:26:48Z
attributes.tag: External Id
attributes.value: SAMEA2071628
attributes.tag: INSDC center name
attributes.value: School of Life Sciences
attributes.tag: INSDC first public
attributes.value: 2013-09-01T17:01:52Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T16:26:48Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-1671:Group VH - Pool YH3
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: honey bee
attributes.tag: developmental stage
attributes.value: pupa
attributes.tag: individual
attributes.value: YH3
attributes.tag: sample name
attributes.value: E-MTAB-1671:Group VH - Pool YH3
attributes.tag: scientific_name
attributes.value: Apis mellifera