organism: Nicotiana benthamiana
description: Protocols: Leaf tissues were excised and flash frozen in liquid nitrogen plants were grown in controlled environment at 22°C with a photoperiod of 16h light / 8h dark and a photosynthetically active radiation of 90 µmol m-2 sec-1 provided by cool white fluorescent tubes. GFP fluorescence was monitored using a hand-held 1 000 W long-wavelength UV lamp (B1000 AP; Ultraviolet Products, Upland, CA, USA). Total RNA were extracted from leaf tissues grinded in liquid nitrogen using the TRIzol™ (Invitrogen™, ThermoFisher Scientific, Waltham, MA, USA) reagent method following manufacturer instructions. For each RNA samples (WT, GFP6.4, NS, OS and SS), librairies were were constructed using 3µg of pooled total RNAs extracted from four individual plants. Library preparation and sequencing were performed at GenXPro (GmbH, Frankfurt am Main, Germany) as described in (Zawada et al., 2014 Massive analysis of cDNA ends (MACE) and miRNA expression profiling identifies proatherogenic pathways in chronic kidney disease).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-04-19T04:02:41Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-04-09T16:02:42Z
attributes.tag: External Id
attributes.value: SAMEA5560255
attributes.tag: INSDC center name
attributes.value: Laboratory of Plant Molecular Biology Institute of Molecular Biology and Biotechnology Foundation for Research and Technology - Hellas
attributes.tag: INSDC first public
attributes.value: 2019-04-19T04:02:41Z
attributes.tag: INSDC last update
attributes.value: 2019-04-09T16:02:42Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7851:Sample 3
attributes.tag: age
attributes.value: 3
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: developmental stage
attributes.value: 2-3 leaf pair
attributes.tag: genotype
attributes.value: GFP6.4; Phenotype OS
attributes.tag: organism part
attributes.value: leaf
attributes.tag: sample name
attributes.value: E-MTAB-7851:Sample 3
attributes.tag: scientific_name
attributes.value: Nicotiana benthamiana