organism: uncultured soil microorganism
description: Soil samples collected in October 2011 on Site A of the BEF China project. Each soil sample was collected in 20-30 cm distance from a specified sampling tree seedling. Tree seedlings have been planted March/April 2009.
attributes.tag: ENA first public
attributes.value: 2016-02-06
attributes.tag: ENA last update
attributes.value: 2016-10-21
attributes.tag: External Id
attributes.value: SAMEA3697590
attributes.tag: INSDC center alias
attributes.value: HELMHOLTZ CENTRE FOR ENVIRONMENTAL RESEARCH - UFZ.
attributes.tag: INSDC center name
attributes.value: HELMHOLTZ CENTRE FOR ENVIRONMENTAL RESEARCH - UFZ.
attributes.tag: INSDC first public
attributes.value: 2016-02-06T17:05:42Z
attributes.tag: INSDC last update
attributes.value: 2016-10-21T09:49:08Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: 9
attributes.tag: amount or size of sample collected
attributes.value: 15
attributes.tag: base saturation
attributes.value: 14.56
attributes.tag: carbon/nitrogen
attributes.value: 13.85
attributes.tag: cation exchange capacity
attributes.value: 72.97
attributes.tag: collected by
attributes.value: Zhiqin Pei and Christina Weissbecker
attributes.tag: collection date
attributes.value: 2011-10
attributes.tag: composite design/sieving (if any)
attributes.value: one forest plot had the size 25.8 m x 25.8 m with 400 tree individuals planted in 1.29 m distance, at each forest plot four tree individuals of each tree species were randomly selected for sampling, at each sampling tree four soil cores were retrieved at 20-30 cm distance in each direction, all four cores form one composite sample for each sampling tree
attributes.tag: depth
attributes.value: 0.1
attributes.tag: diversity level
attributes.value: 1
attributes.tag: dna extraction kit
attributes.value: two extractions of 0.25 g soil by Power Soil htp 96 well Soil DNA Isolation Kit (MoBio)
attributes.tag: environment (biome)
attributes.value: forest
attributes.tag: environment (feature)
attributes.value: subtropical experimental forest
attributes.tag: environment (material)
attributes.value: soil
attributes.tag: environmental package
attributes.value: soil
attributes.tag: experimental factor
attributes.value: diversity gradient
attributes.tag: geographic location (country and/or sea)
attributes.value: China
attributes.tag: geographic location (elevation)
attributes.value: 188.29
attributes.tag: geographic location (latitude)
attributes.value: 29.12472022
attributes.tag: geographic location (longitude)
attributes.value: 117.9078234
attributes.tag: geographic location (region and locality)
attributes.value: Jiangxi Province, Xingangshan village
attributes.tag: history/previous land use
attributes.value: Cunninghamia lanceolata plantation
attributes.tag: investigation type
attributes.value: mimarks-survey
attributes.tag: iron exchange capacity
attributes.value: 0
attributes.tag: mycorrhiza type sampling tree
attributes.value: AM
attributes.tag: note
attributes.value: soil
attributes.tag: ph_h2o
attributes.value: 4.53
attributes.tag: plot id
attributes.value: K19
attributes.tag: sample collection device or method
attributes.value: metal soil corer of 6 cm diameter and 10 cm height
attributes.tag: sample material processing
attributes.value: removal of roots and stones,sieving 2 mm mesh, homogenization of 4 soil cores collected around one sampling tree, snap-freezing of about 15g soil in liquid nitrogen, transport on dry ice and storage at -80�C, freeze-drying before transport and relocation to -80�C upon arrival four days later
attributes.tag: sample name
attributes.value: 9
attributes.tag: sample weight for DNA extraction
attributes.value: 0.5
attributes.tag: sampling tree
attributes.value: Schima_superba
attributes.tag: slope
attributes.value: 26.83
attributes.tag: soil taxonomic/fao classification
attributes.value: Cambisol
attributes.tag: total organic carbon
attributes.value: 3.68
attributes.tag: url
attributes.value: http://www.bef-china.de
attributes.tag: water content
attributes.value: 48.02