organism: Phaeodactylum tricornutum
description: Protocols: P.tricornutum cells from three biological replicates were harvested in the middle of light period by vacuum filtration on 1.2 um polycarbonate filters (Millipore, United States) long time(8 months). P. tricornutum cells were harvested in the middle of the light period by vacuum filtration onto 1.2 um polycarbonate filters (Millipore, United States), then flash frozen in liquid N2 and maintained at -80C until RNA extraction. The LC sample comprised three biological replicates and HC sample comprised three biological replicates. P.tricornutum cells from three biological replicates were harvested in the middle of light period by vacuum filtration on 1.2 um polycarbonate filters (Millipore, United States) long time(8 months). P. tricornutum cells were harvested in the middle of the light period by vacuum filtration onto 1.2 um polycarbonate filters (Millipore, United States), then flash frozen in liquid N2 and maintained at -80C until RNA extraction. The LC sample comprised three biological replicates and HC sample comprised three biological replicates. Total RNA was extracted from the frozen cell pellets using the TRIzol Reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions. Total RNA was extracted using the mirVana miRNA Isolation Kit (Ambion) following the manufacturer's protocol. RNA integrity was evaluated using the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). The samples with RNA Integrity Number (RIN) 7 or higher were subjected to the subsequent analysis. The libraries were constructed using TruSeq Stranded mRNA LTSample Prep Kit (Illumina, San Diego, CA, USA) according to the manufacturer's instructions.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-11-07T17:04:19Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-09-20T11:47:30Z
attributes.tag: External Id
attributes.value: SAMEA5789186
attributes.tag: INSDC center name
attributes.value: Marine Life College, Ocean University of China
attributes.tag: INSDC first public
attributes.value: 2019-11-07T17:04:19Z
attributes.tag: INSDC last update
attributes.value: 2019-09-20T11:47:30Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8351:Sample LC1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: growth condition
attributes.value: 390 ppmv CO2 environment
attributes.tag: organism part
attributes.value: whole organism
attributes.tag: sample name
attributes.value: E-MTAB-8351:Sample LC1
attributes.tag: scientific_name
attributes.value: Phaeodactylum tricornutum
attributes.tag: strain
attributes.value: NEPCC640
attributes.tag: time
attributes.value: 8