organism: Saccharomyces cerevisiae
attributes.tag: source_name
attributes.value: saccharomyces cerevisiae cells
attributes.tag: strain
attributes.value: BY4741 ecm33-delta-intron ubc4-delta-intron hnt1-delta-intron sac6-delta-intron rfa2-delta-intron
attributes.tag: growth condition
attributes.value: Culture was seeded at OD600 0.2 from overnight culture, allowed to grow to early log phase (4h), collected by vacuum filtration, resuspended in fresh media supplemented with 100 nM rapamycin, and harvested after 4h.
attributes.tag: treatment
attributes.value: pre-growth in SC, growth in SC+rapamycin (100 nM)