organism: hydrocarbon metagenome
description: Sample was obtained from a core sample drilled at a Northern Alberta reservoir in February 2008. The core sample represented from 595 to 625 metres below surface.  Subsamples were collected from this core by taking 4 cm sections from selected depths.  Material for use in DNA extraction was scrapred from the center area of the 4cm subsample. DNA was extracted from 1-2 g of this scraped material using the MP Biomedicals FastDNA Spin Kit for Soil. 2 rounds of PCR were used, one with universal non-bar coded primers and the second round with titanium amplicon primers,  to generate amplicons from the obtained DNA.