organism: Hypocreales sp. ML197-S3
attributes.tag: strain
attributes.value: ML197-S3
attributes.tag: isolation_source
attributes.value: coastal sea water
attributes.tag: collection_date
attributes.value: 2015-08-01/2015-08-08
attributes.tag: env_broad_scale
attributes.value: mediterranean sea biome [ENVO_01000047]
attributes.tag: env_local_scale
attributes.value: environment associated with an aquatic invertebrate [ENVO_01001176]
attributes.tag: env_medium
attributes.value: Protula[NCBITaxon_51281]|coastal sea water [ENVO_00002150]
attributes.tag: geo_loc_name
attributes.value: Spain:Granada
attributes.tag: host
attributes.value: Protula
attributes.tag: isol_growth_condt
attributes.value: PMID: 32392868
attributes.tag: lat_lon
attributes.value: 36.723083 N 3.726417 W
attributes.tag: host_body_habitat
attributes.value: Protula intestinum, Aphia ID: 131032
attributes.tag: samp_collect_device
attributes.value: host collection: scuba diving
attributes.tag: samp_mat_process
attributes.value: After sampling, the invertebrate was transferred carefully to the laboratory to be processed. Small pieces of tissue were taken from the sample before freezing. 1 cm3 samples were ground in sterile seawater and heated at 50C for 1 h. The suspension was serially diluted, plated on Difco Marine Broth Agar (BD Biosciences San Jose, CA, USA), and incubated at 28C for six weeks. A single colony was picked from the agar and cultivated as a pure culture on Difco Potato Dextrose Agar and Difco Marine Broth Agar (BD Biosciences San Jose, CA, USA) (media and cultivated for five days at 28C. The strain spores and mycelium were recovered by a gentle scratch of the agar plate surface using a scalpel, and they were conserved at 20C in 10% glycerol solution.
attributes.tag: samp_salinity
attributes.value: sea water salinity: 38
attributes.tag: samp_store_temp
attributes.value: host tissue: -20C, fungal isolate: -80C
attributes.tag: temp
attributes.value: sea water temperature: 21C - 27C
attributes.tag: BioSampleModel
attributes.value: MIGS.eu
attributes.tag: BioSampleModel
attributes.value: MIGS/MIMS/MIMARKS.host-associated