organism: Escherichia coli str. K-12 substr. MG1655
description: Protocols: An overnight culture of E. coli TOP10-pBIC1a was diluted 100-fold in fresh BHI broth and allowed to grow to an OD600 of 0.5. The culture was then split and imipenem (Imipenem-Cilastatin, Mylan, USA) was added to one of the cultures at a final concentration of 5 µg/ml. Ten minutes later, 2 ml of each culture were taken and mixed with RNA protect (Qiagen, Courtaboeuf, France) according to the manufacturer's recommendations. Cells were immediately pelleted by a 10 min centrifugation at 5000 g. RNA was extracted using GeneJet RNA extraction kit (ThermoScientific). DNAse treatment was performed with the dsDNAse kit (ThermoScientific) cDNA libraries were prepared using the NEBNext® Ultra Directional RNA Library Prep Kit for Illumina protocol (New England Biolabs, MA, USA). The quality of the cDNA was validated using the Agilent 2100 Bioanalyzer DNA1000 kit (Agilent Technologies) and quantity was determined with the Qubit dsDNA BR Assay (ThermoFisher Scientific).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-09-15T17:01:49Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-09-05T15:48:59Z
attributes.tag: External Id
attributes.value: SAMEA4886519
attributes.tag: INSDC center name
attributes.value: Bicetre Hospital, EA7361
attributes.tag: INSDC first public
attributes.value: 2018-09-15T17:01:49Z
attributes.tag: INSDC last update
attributes.value: 2018-09-05T15:48:59Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7190:Sample 2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: genotype
attributes.value: pBIC1a
attributes.tag: growth condition
attributes.value: cell suspension culture
attributes.tag: sample name
attributes.value: E-MTAB-7190:Sample 2
attributes.tag: scientific_name
attributes.value: Escherichia coli K-12
attributes.tag: strain
attributes.value: K-12
attributes.tag: substrain
attributes.value: MG1655