organism: Mus musculus
description: Untreated sample. The AtT-20 cell line is an anterior pituitary corticotroph of murine origin (ATCC). Cell lines were maintained in Dulbecco's Modified Eagle Medium (DMEM) (Invitrogen,  Carlsbad, CA) supplemented with 10% fetal bovine serum (Gemini, Woodland,  California), 2 mM L-glutamine, 1 mM sodium pyruvate, 0.1 mM non-essential amino  acids, 5 mg/ml penicillin-streptomycin (Invitrogen, Carlsbad, CA) and kept at 37oC  incubator with 5% CO2.