organism: Apocynum
description: The experiment was carried out in the shelter covered by a transparent film that blocked rainwater and other conditions were similar to the open-air environment in Nanjing University of Chinese Medicine. The main roots of Apocynum venetum L. (two years old and originated from the same plant) were planted in a pot containing 25 kg of soil. Each pot contains three roots. Salt stress tests were conducted when Apocynum venetum L. was about 30 cm height. Four groups were exposed to different levels of salt treatment, 0 (control, watering), 100 (low stress), 200 (moderate stress) and 300 (high stress) mM NaCl, respectively. Each group was designed with 3 replicates and 3 pots per replicate. Each pot was poured by 2 L of solution according to preliminary experiments. NaCl concentration was increased gradually by 50 mM every four days to reduce osmotic shock until the designated concentration was reached. The treatments were lasted for 6 times (20 days) under four levels of NaCl. After the last salt treatments, leaves were harvested. After being quick frozen in liquid nitrogen, these leaves were transferred to a -80 C refrigerator for storage.
attributes.tag: isolate
attributes.value: pick
attributes.tag: cultivar
attributes.value: Luobuma
attributes.tag: ecotype
attributes.value: The production area is Jiangsu Province
attributes.tag: age
attributes.value: Flowering period
attributes.tag: dev_stage
attributes.value: Traditional harvest period
attributes.tag: geo_loc_name
attributes.value: China: jiangsu
attributes.tag: tissue
attributes.value: leaves
attributes.tag: treatment
attributes.value: watering
attributes.tag: replicate
attributes.value: 1
attributes.tag: BioSampleModel
attributes.value: Plant