organism: hydrocarbon metagenome
description: Samples from a oil and gas company's seawater Injection system were sent with the objective of 16S pyrosequencing. Six seawater samples were filtered on-site and the filter paper was fixed with particular fixative and dried. Similarly, three seawater samples from crude pipeline were also filtered on-site, filter paper fixed with fixative and dried. Each of the nine fixed filter papers were transferred in a 2 ml Eppendorf tube immersed in 1.5 ml filtrate and sent for 16S pyrosequencing. Samples # 1, 2, 3, 4, 5 and 6 were seawater samples, while samples # 7A, 7B and 8 were seawater samples from crude pipeline (as mentioned with the document included with the samples). On receiving the samples, the Eppendorf tubes were strongly vortexed to get all the biomass into the solution (seawater filtrate). This was repeated 2-3 times to be sure that all the biomass could be recovered from the fixed filter paper. Next, the tubes were centrifuged at 17,000 x g for 10 minutes. The supernatant was discarded and the cell pellet was frozen at -70* till DNA extraction.