organism: Mus musculus
description: Protocols: mESC-derived neurons were grown in the presence of 200nM 4-thiouridine (s4U) for 24 hours, then s4U was replaced with unmodified uridine (U); Soma/neurite compartments were separated by the lab standard protocol (1 filter/replicate). RNA was isolated at the indicated time points, where 0 hours is the time of s4U withdrawal. Isolated RNA was subjected to alkylation using SLAMseq Kinetics kit (Lexogen 062.24) according to the manufacturer's recommendations. Libraries were prepared from 70-200ng of input RNA using QuantSeq 3' mRNA-Seq Library Prep Kit (REV) for Illumina (Lexogen 016.24).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-12-24T04:05:24Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-07-29T17:27:12Z
attributes.tag: External Id
attributes.value: SAMEA7098588
attributes.tag: INSDC center name
attributes.value: Berlin Institute for Medical Systems Biology
attributes.tag: INSDC first public
attributes.value: 2020-12-24T04:05:24Z
attributes.tag: INSDC last update
attributes.value: 2020-07-29T17:27:12Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-9400:SLAM_T0_S3
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: Ascl1-induced neurons (iNeurons)
attributes.tag: cell type
attributes.value: neuron
attributes.tag: common name
attributes.value: house mouse
attributes.tag: disease
attributes.value: normal
attributes.tag: fraction
attributes.value: soma
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: sample name
attributes.value: E-MTAB-9400:SLAM_T0_S3
attributes.tag: scientific_name
attributes.value: Mus musculus