organism: Mus musculus
description: Protocols: Mice culled by anaesthetizing with isofluorane followed by cervical dislocation. Brain removed by cutting coronally at approximately bregma -0.1 and -3.0. The slice was orientated with the caudal side face up and a hypothalamic punch of 3 mm in diameter was taken from the ventral aspect. Punches were transferred to a microfuge tube immediately and frozen on dry ice. Tissue was stored at -70 degrees Celsius until processed. Sixteen wild type C57BL/6J mice, 3-4 weeks old (Harlan), singly housed and acclimatized (12:12 light:dark) on low fat diet (10% fat) for two weeks. Eight mice then switched to high fat diet (60% fat) and eight mice remain on low fat diet (10% fat). Diets continued for 20 weeks. RNA was extracted from the whole hypothalamic block using Machery-Nagel RNA isolation kit as per manufacturer's instructions. Libraries were prepared for sequencing using the Illumina TruSeq Stranded mRNA kit.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-10-23T17:03:42Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-06-21T10:02:11Z
attributes.tag: External Id
attributes.value: SAMEA4752151
attributes.tag: INSDC center name
attributes.value: Centre for Genome Enabled Biology and Medicine University of Aberdeen
attributes.tag: INSDC first public
attributes.value: 2018-10-23T17:03:42Z
attributes.tag: INSDC last update
attributes.value: 2018-06-21T10:02:11Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6929:17
attributes.tag: age
attributes.value: 25 to 26
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: diet
attributes.value: high fat diet
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: hypothalamus
attributes.tag: sample name
attributes.value: E-MTAB-6929:17
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: C57BL/6J