organism: Corynebacterium glutamicum
description: Protocols: Strain ATCC 13032, harvesting cells after cultivation and/or sorting using a FACS device. First the cells were grown in BHI complex media for 5 to 6 hours. After this, the cells that were starved to iron by cultivating them under low iron conditions (1 µM) over night. In the next day the cells were used to inoculate a new culture under normal iron conditions (36 µM). In the iron shift experiments a plasmid based reporter was used. This reporter system consists of the venus gene cloned under the control of a promoter of the SOS-response gene divS. For large volumes: RNeasy Kit (Qiagen, Hilden, Germany), cell disruption was performed with glass beads For small volumes (sorted samples): NucleoZol (Machery-Nagel, Düren, Germany) was used, cell disruption was performed with lysozyme + mutanlysin large sample volumes: rRNA depletion was done using Ribo-Zero rRNA Removal Kit (Gram-Positive Bacteria) (Epicentre/illumina, Munich, Germany) small volumes: a Protocol for Removal of rRNA from Small Amounts of Total RNA” from Clontech used All libraries were generated using: TruSeq Stranded emRNA Library Prep Kit (Illumina, Munich, Germany)
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-09-15T17:01:47Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-05-29T15:57:38Z
attributes.tag: External Id
attributes.value: SAMEA4697087
attributes.tag: INSDC center name
attributes.value: IBG-1, Forschungszentrum Julich GmbH
attributes.tag: INSDC first public
attributes.value: 2018-09-15T17:01:47Z
attributes.tag: INSDC last update
attributes.value: 2018-05-29T15:57:38Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6809:Sample 33
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: genotype
attributes.value: divS-Venus
attributes.tag: phenotype
attributes.value: negative
attributes.tag: replicate
attributes.value: 1
attributes.tag: sample name
attributes.value: E-MTAB-6809:Sample 33
attributes.tag: scientific_name
attributes.value: Corynebacterium glutamicum
attributes.tag: stimulus
attributes.value: iron shift
attributes.tag: strain
attributes.value: ATCC 13032
attributes.tag: type-material
attributes.value: type strain of Corynebacterium glutamicum