organism: marine sediment metagenome
attributes.tag: isolation_source
attributes.value: marine sediment
attributes.tag: collection_date
attributes.value: Not applicable
attributes.tag: geo_loc_name
attributes.value: North Sea: German Bight (Helgoland Mud Area)
attributes.tag: lat_lon
attributes.value: 54.052300 N 7.580400 E
attributes.tag: rel_to_oxygen
attributes.value: obligate anaerobe
attributes.tag: samp_collect_device
attributes.value: anoxic sampling of sediment slurry incubations at defined time points
attributes.tag: samp_mat_process
attributes.value: Previously collected sediments from Helgoland mud area, stored at 4ºC (near in-situ temperature) until use as starting material for incubation experiments. For the incubation experiments, anaerobic slurries were prepared in ratio 1:4 and incubated at 30°C, sample name identifies the unique information of each sample including the target 16S rRNA gene and incubation timepoint.
attributes.tag: samp_size
attributes.value: ~0.5 g sediment pellets in triplicates, Pooled DNA from triplicates were sequenced as 1 sample.
attributes.tag: source_material_id
attributes.value: Day_250 methane amended sample from methanic zone sequenced for Archaeal 16S rRNA gene
attributes.tag: BioSampleModel
attributes.value: Metagenome or environmental