organism: Homo sapiens
description: Protocols: All animal experiments were performed to the according to the Home Office UK. HCC70 cells (1x106 cells in RPMI serum free medium mixed 50:50 with MatrigelTM) tumours were implanted in the flank of female nude mice (nu/nu:Alpk) (AstraZeneca, Alderley Park, UK) between the ages of 8 and 12 weeks. Once tumours reached ~200-500mm3 animals were randomised into control and treatment groups. Tumour volume was calculated twice weekly from bilateral caliper measurements using the formula (Length x width x width) x π/6). Dosage of treated xenografts For xenograft models, cell pellets and tissues from xenograft models were snap frozen. Total RNA was extracted using miRNeasy kit (Qiagen), with DNAse treatment, following manufacturer's instructions. RNA quantity was assessed by Nanodrop 2000. Samples profiled for RNA-seq were assessed for RNA integrity (RIN>7) using the RNA 6000 Nano Assay on the BioAnalyser (Agilent). Sample were sent for RNA-sequencing at Liverpool Centre for Genomic Research. Total RNA was ribo-depleted, paired-end ScriptSeq library was performed and sequenced for 100bp/100M reads per sample.
attributes.tag: ENA first public
attributes.value: 2017-11-01
attributes.tag: ENA last update
attributes.value: 2016-06-01
attributes.tag: External Id
attributes.value: SAMEA4019864
attributes.tag: INSDC center alias
attributes.value: AZ
attributes.tag: INSDC center name
attributes.value: AstraZeneca
attributes.tag: INSDC first public
attributes.value: 2017-11-01T17:02:33Z
attributes.tag: INSDC last update
attributes.value: 2016-06-01T15:27:43Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-4656:HCC70_100_2hr_B2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: HCC70
attributes.tag: common name
attributes.value: human
attributes.tag: sample name
attributes.value: E-MTAB-4656:HCC70_100_2hr_B2
attributes.tag: sampling site
attributes.value: flank
attributes.tag: xenograft
attributes.value: breast cancer cell line
attributes.tag: xenograft host organism
attributes.value: mouse
attributes.tag: xenograft strain
attributes.value: nu/nu:Alpk