organism: Drosophila melanogaster
description: adult fly line expressing GFP Mago, RNAseq biological replicate 2. mRNA was extracted from whole flies using Trizol LS (Ambion) according to manufacture's protocol. 10μg of purified total RNA were depleted from rRNA and small RNAs through two consecutive poly-A mRNA capture steps, using oligo-dT25 coated magnetic Dynabeads (Sigma-Aldrich) according to manufactures protocol. 500ng purified mRNA were submitted for library preparation. Barcoded stranded mRNA-seq libraries were prepared using the Illumina TruSeq RNA Sample Preparation v2 Kit (attached, Illumina, San Diego, CA, USA) implemented on the liquid handling robot Beckman FXP2.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-08-02T17:03:12Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-04-25T22:32:45Z
attributes.tag: External Id
attributes.value: SAMEA4609857
attributes.tag: INSDC center name
attributes.value: EMBL
attributes.tag: INSDC first public
attributes.value: 2018-08-02T17:03:12Z
attributes.tag: INSDC last update
attributes.value: 2018-04-25T22:32:45Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: RNAseq_3A2_rep2
attributes.tag: common name
attributes.value: fruit fly
attributes.tag: sample name
attributes.value: RNAseq_3A2_rep2
attributes.tag: scientific_name
attributes.value: Drosophila melanogaster