organism: human metagenome
description: Fragments of bacterial 16S rRNA gene were amplified from 194 human patient or control samples (Forward primer 5'-ACTCCTACGGGAGGCAGCAG-3': Reverse primer: 5'-CCGTCAATTCMTTTGAGTTT-3'). PCR products were barcoded and sequenced in three 454 runs. Bar code sequences are provided as run attributes.   Only sequences with perfect matches to 454 MIDs and sequence specific primers were retained. Sequences containing ambiguity codes and homopolymers longer than 7 nt, singleton sequences (sequences present only once in all 194 samples) were also filtered out.