organism: Mus musculus
description: Protocols: Experiments were carried out in agreement with European Community Council Directive (86/609/EEC) and the animal ethics guidelines of the i3S, and approved by the Portuguese Veterinary Ethics Committee. Dorsal spinal cord tissues were collected from E14.5 embryos and then dissected in ice-cold PBS. Cells were fixed sequentially with di(N-succimidyl) glutarate and 1% formaldehyde in phosphate buffered saline and then lysed, sonicated and immunoprecipitated, as previously described (Castro et al., 2011; Regadas et al, 2014), with an anti-Tlx3 antibody DNA libraries were prepared from 10ng of immunoprecipitated DNA according to the standard Illumina ChIP-seq protocol.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-06-14T04:02:39Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-07-04T15:12:48Z
attributes.tag: External Id
attributes.value: SAMEA4776692
attributes.tag: INSDC center name
attributes.value: Instituto Gulbenkian de Ciencia
attributes.tag: INSDC first public
attributes.value: 2019-06-14T04:02:39Z
attributes.tag: INSDC last update
attributes.value: 2018-07-04T15:12:48Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6974:Sample 1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: neuron
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: embryonic day 14.5
attributes.tag: organism part
attributes.value: dorsal spinal cord
attributes.tag: sample name
attributes.value: E-MTAB-6974:Sample 1
attributes.tag: scientific_name
attributes.value: Mus musculus