organism: Homo sapiens
description: Human induced pluripotent stem cell line derived from foreskin fibroblasts, isolated as described by Yu, J. et al. Human induced pluripotent stem cells free of vector and transgene sequences. Science 324, 797-801 (2009). For differentiation of the iPS cells into trophoblast lineage using BMP4, the cells were grown in 10cm2 dishes (approximately 1 x 10e7 cells/dish) in feeder free conditions on Matrigel using mTeSR1 media containing 50 ng/ml BMP4 for 5 days (RND systems, Minneapolis, MN).