organism: Mus musculus
description: sequencing of miRNA from wild type and diseased mouse hearts using PCR-based bar-codes. Samples of cardiac left ventricle were taken from three wild-type mouse, in addition a pooled sample was created. Samples of cardiac left ventricle were also taken from three diseased mouse (Hypertrophic cardiomyopathy), in addition a pooled sample was created. miRNA were extracted from these eight samples using the AMbion mirVana miRNA Isolation Kit (Ambion AM1561). The eight libraries were sequenced together using PCR-based bar-codes in one lane of illumina GAIIx instrument.