organism: Alexandrium minutum
description: Alexandrium minutum strain CCMP113 was obtained from the CCMP culture collection (https://ccmp.bigelow.org/) and grown in L1 media, at 16 *C, under a 12/12 light cycle, and a photon irradiance of ~100 mmol photons m-2 s-1. To isolate total RNA for 454-library construction the culture was harvested in exponential growth phase through centrifugation (1 min, 1000 x g, 12 *C). Cells were washed twice with PBS, exposed to bead-beating on dry ice with the Fast Prep bead-beater from Medinor (20 s, speed 4) using 1.4 mm beads (Medinor) and total RNA was extracted with the ChargeSwitch* Total RNA Cell kit (Invitrogen) according to the manufacturers’ protocol. Normalized polyA-enriched cDNA libraries with 454 adapters attached at each end were constructed commercially by Vertis Biotechnologie AG (http://www.vertis-biotech.com/). Adapter sequences were: GACCTTGGCTGTCACTCA (5') and TCGCAGTGAGTGACAGGCCA (3'). Two runs, a quarter of a plate each, were sequenced at the Norwegian High-Throughput Sequencing Centre (http://www.sequencing.uio.no/)using Roche 454 sequencing TITAN technology.