title: Identification of Taxus microRNAs with high-throughput sequencing
description: Total RNA was extracted from the fresh leaves of pot-cultivated Taxus mairei using Trizol (Invitrogen). The samples were then subjected to 15% denaturing polyacrylamide gel electrophoresis (PAGE), after which the small RNA fragments of 18–30 nt were isolated from the gel and purified.