title: Wild-type
organism: Trypanosoma brucei brucei
description: Protocols: T. brucei bloodstream form parasites (strain Lister 427, antigenic type MiTat 1.2, clone 221a) [Johnson and Cross, 1979] were cultured at 37 degrees C, 5% CO2, in HMI-11 medium. RNA interference of histone H1 was induced for 4 days in culture. Total RNA was extracted using TRIzol standard protocol. Ribosomal RNA was depleted with RiboMinus kit (Invitrogen). PolyA-containing RNA was purified using Dynabeads oligo-(dT) (Invitrogen) and cDNA was synthesized using random hexamers according to Complete RNA-seq Library System kit (NuGEN) instructions.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2014-06-10T17:03:23Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2013-06-11T10:56:05Z
attributes.tag: External Id
attributes.value: SAMEA2078113
attributes.tag: INSDC center name
attributes.value: Lab manager and bioinformatician
attributes.tag: INSDC first public
attributes.value: 2014-06-10T17:03:23Z
attributes.tag: INSDC last update
attributes.value: 2013-06-11T10:56:05Z
attributes.tag: INSDC status
attributes.value: suppressed
attributes.tag: Submitter Id
attributes.value: E-MTAB-1715:Wild-type
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: developmental stage
attributes.value: bloodstream slender form
attributes.tag: genotype
attributes.value: wild type
attributes.tag: sample name
attributes.value: E-MTAB-1715:Wild-type
attributes.tag: scientific_name
attributes.value: Trypanosoma brucei brucei
attributes.tag: specimen with known storage state
attributes.value: fresh specimen
attributes.tag: strain
attributes.value: Lister 427
attributes.tag: sub_species
attributes.value: brucei