title: Mt_NSP1_MYCS_10-6M_2H+4H_R3
organism: Medicago truncatula
attributes.tag: Title
attributes.value: Mt_NSP1_MYCS_10-6M_2H+4H
attributes.tag: Strain
attributes.value: Jemalong
attributes.tag: Genotype
attributes.value: NSP1
attributes.tag: Molecule
attributes.value: total_RNA
attributes.tag: Extract_protocol
attributes.value: 2.5 cm long root samples were harvested 1 mm above the root tip. Total RNA was extracted using the Qiagen RNeasy Plant mini kit (Qiagen, Hilden, Germany). RNA quality was assessed using the Agilent 2100 BioAnalyzer technology (Agilent technologies, Santa Clara, USA).
attributes.tag: Growth_protocol
attributes.value: Medicago truncatula cv Jemalong nsp1-1 mutant (Catoira et al., 2000) was grown on Phytagel-solidified M medium (Becard and Fortin, 1988) at 21°C (70% humidity, 150 µmol m-2 s-1 photosynthetic photon flux, 16 h light period) for 4 days.