title: Phosphorus and Iron Co-limited
organism: Thalassiosira pseudonana CCMP1335
description: Axenic T. pseudonana CCMP 1335 was grown at 14*C under 24 hour light (120 *mol photons m-2 s-1) after Dyhrman et al. (2012) in f/2 plus silica chelated media made from surface Sargasso Sea water. Nitrate, silica, vitamins, and trace metals were at f/2 concentrations.  Iron and phosphate were modified from the f/2 concentrations for the Co-limited treatment to 0.4 uM PO4 and 40 nM Fe.