title: control_0_1
organism: Mus musculus
description: Protocols: Cardiomyocytes were isolated from neonatal mice and seeded on gelatin. In particular, Mouse neonatal cardiomyocytes were isolated using Worthington Rat neonatal isolation kit. Briefly, hearts were isolated from 1‐ to 4‐day‐old BALB/c mice, minced, and incubated overnight at 4°C. Trypsin inhibitor was added, and heart pieces were incubated for 35 min in the presence of collagenase at 37°C. Cells were passed through 100‐μm cell strainer, fibroblasts were removed by adhesion for 2 h, and non‐adhered cells were centrifuged (50 g/5 min) and seeded at concentration of 3 × 104 cells/well in gelatin‐coated 6‐well plates in a myocytes growth medium (promocell) for 5 days. ShRNA was delivered by lentiviruses and LPS stimulation was given after 72 hours. The results are of two biological replicates. 500 ng LPS stimulation for 2 and 4 hours RNA was extracted using Norgen RNA isolation kit. nebnext ultra rna seq kit, multiplexed
attributes.tag: ENA first public
attributes.value: 2017-06-13
attributes.tag: ENA last update
attributes.value: 2017-06-13
attributes.tag: External Id
attributes.value: SAMEA104121248
attributes.tag: INSDC center alias
attributes.value: Faculty of medicine in safed, Bar-Ilan university
attributes.tag: INSDC center name
attributes.value: Faculty of medicine in safed, Bar-Ilan university
attributes.tag: INSDC first public
attributes.value: 2017-06-13T17:01:25Z
attributes.tag: INSDC last update
attributes.value: 2017-06-13T14:54:21Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-5826:control_0_1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: cardiac myocyte
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: mouse postnatal
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: sample name
attributes.value: E-MTAB-5826:control_0_1
attributes.tag: stimulus
attributes.value: no stimulus
attributes.tag: time
attributes.value: 0