title: DS_20
organism: Mycobacterium tuberculosis H37Rv
description: Protocols: M. tuberculosis H37Rv (ATCC 25618) was grown at 37C in modified Dubos medium (Difco) supplemented with 4% Dubos medium albumin (Difco) and 0.5% w/v glycerol. RNA was prepared using a FastRNA pro blue kit (Qbiogene). Contaminating DNA was removed using a TURBO DNA-free kit (Ambion) 2-3 ug of total RNA were fragmented (Ambion Cat # AM8740) and strand-specific cDNA libraries were constructed using the Illumina directional mRNA-Seq protocol (Part # 15018460 Rev. A) but with exclusion of polyA-tail and size selection to capture all RNA species. Briefly, this protocol ligates the Illumina v1.5 small RNA 3prime adapter followed by a 5prime adapter to preserve strand specificity.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2013-09-17
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2013-05-16
attributes.tag: External Id
attributes.value: SAMEA2072011
attributes.tag: INSDC center alias
attributes.value: MRC National Institute for Medical Research
attributes.tag: INSDC center name
attributes.value: MRC National Institute for Medical Research
attributes.tag: INSDC first public
attributes.value: 2013-09-17T17:00:33Z
attributes.tag: INSDC last update
attributes.value: 2013-05-16T10:00:56Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-1667:DS_20
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: genotype
attributes.value: HigB overexpressing
attributes.tag: sample name
attributes.value: E-MTAB-1667:DS_20
attributes.tag: specimen with known storage state
attributes.value: fresh specimen
attributes.tag: strain
attributes.value: H37Rv
attributes.tag: type-material
attributes.value: type strain of Mycobacterium tuberculosis