title: Sample 1
organism: Homo sapiens
description: Protocols: Exponentially growing HAP1 cells in monolayer were harvested by trypsinization and then centrifugation. Cell pellets were washed twice in phosphate buffered saline (PBS) and then stored at -80 degrees Celsius until required for nucleic acid extraction. Nucleic acid was extracted from cell pellets using Trizol (Invitrogen), and the total RNA fraction was retained and purified according to manufacturerâ€™s instructions. polyA+ RNA was purified from total RNA samples, and a PCR-amplified library was prepared using the Illumina TruSeq RNA stranded library preparation kit.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-08-06T17:02:14Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-07-27T11:30:25Z
attributes.tag: External Id
attributes.value: SAMEA4807016
attributes.tag: INSDC center name
attributes.value: UZH
attributes.tag: INSDC first public
attributes.value: 2018-08-06T17:02:14Z
attributes.tag: INSDC last update
attributes.value: 2018-07-27T11:30:25Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7089:Sample 1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: HAP-1
attributes.tag: cell type
attributes.value: fibroblast
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: chronic myelogenous leukemia
attributes.tag: organism part
attributes.value: bone marrow
attributes.tag: sample name
attributes.value: E-MTAB-7089:Sample 1
attributes.tag: scientific_name
attributes.value: Homo sapiens