title: High throughput detection of gRNA activity in HEK293T cells
organism: Homo sapiens
description: we performed a genome-scale screen to measure gRNA activity for two highly specific SpCas9 variants (eSpCas9(1.1) and SpCas9-HF1) and wild-type SpCas9 (WT-SpCas9) in human cells, and obtained indel rates of over 50,000 gRNAs for each nuclease, covering ~20,000 genes.
attributes.tag: isolate
attributes.value: HEK293T
attributes.tag: age
attributes.value: fetus
attributes.tag: biomaterial_provider
attributes.value: ATCC
attributes.tag: sex
attributes.value: female
attributes.tag: tissue
attributes.value: embryonic kidney
attributes.tag: cell_line
attributes.value: HEK293T
attributes.tag: BioSampleModel
attributes.value: Human