title: Microbial zonation in a bioreactor modeling a souring low temperature oil reservoir subjected to nitrate injection.
organism: hydrocarbon metagenome
description: Samples collected across the flow path of sand-packed bioreactor injected with sulfate and nitrate. Originally inoculated with an enrichment culture from production waters of a low-temperature oil reservoir. DNA was extracted from each of the sand-pack fractions and the 16S community composition was analyzed using PCR-based techniques such as denaturant gradient gel electrophoresis, 454 pyrosequencing and quantitative PCR.