title: 29_TRAR
organism: Homo sapiens
description: Protocols: Melanoma specimens were obtained during surgical interventions, and grown in vitro in serum-free EGF/bFGF-containing medium as described previously (Sztiller-Sikorska et al., 2012 Melanoma Research). Cell resistance was developed for 10uM vemurafenib or 50 nM trametinib. DNA was extracted using DNeasy Blood & Tissue Kit from Qiagen according to manufacturer protocol. DNA samples were quantified using Picogreen (Invitrogen Thermo Fisher Scientific, Carlsbad, CA, USA) and resolved by 1% agarose gel electrophoresis (30 min, 100 V) to confirm the presence of high molecular weight fragments. DNA samples were then prepared according to an Agilent SureSelect Human All Exome V6 kit (Agilent Technologies) which is a solution-based system utilizing ultra-long 120-mer biotinylated cRNA baits to capture regions of interest. Targeted regions were selected using magnetic streptavidin beads, amplified and loaded on the sequencer.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-10-20T17:02:10Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-09-28T18:02:15Z
attributes.tag: External Id
attributes.value: SAMEA4947710
attributes.tag: INSDC center name
attributes.value: Department of Molecular Biology of Cancer, Medical University of Lodz, Poland
attributes.tag: INSDC first public
attributes.value: 2018-10-20T17:02:10Z
attributes.tag: INSDC last update
attributes.value: 2018-09-28T18:02:15Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7248:29_TRAR
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: melanoma
attributes.tag: drug resistance
attributes.value: trametinib resistance
attributes.tag: individual
attributes.value: 29
attributes.tag: organism part
attributes.value: skin
attributes.tag: sample name
attributes.value: E-MTAB-7248:29_TRAR
attributes.tag: scientific_name
attributes.value: Homo sapiens