title: Microbial community from the 2 m depth of an inactive oil sand taililngs pond in 2009
organism: hydrocarbon metagenome
description: Anaerobic sample collected from 2 mbs of an inactive pond in the Athabasca region of northern Alberta. Three sub-samples of approximately 0.5 g of tailings were used for DNA extraction by Fast DNA spin kit for soil. A first PCR round of 25 cycles was done to each separate subsample using 16 S universal primers 926Fw/1392R. The product of this PCR was used as the DNA template for a second PCR round of 25 cycles using reverse bar-coded primers. The resulting DNA was cleaned with Qiagen PCR purification kit and the three subsamples were mixed together as a unique sample which was then sequenced.