title: S2_mRNAseq_siCtrl_2
organism: Drosophila melanogaster
description: Protocols: S2 cells were grown at room temperature in Drosophila Schenider's medium containing 10% fetal bovine serum. For knockdown, one million S2 cells were plated per 6 well in 2m of serum containing medium. After 2-3h, 25ug of dsRNA were dissolved in 1ml of serum free medium, which was added to the cells. Cells were soaked for 30 minutes, after that 2ml of additional serum containing medium was added. Cells were lysed 3 days later. RNA was extracted from cell pellets using Maxwell RSC simply RNA cells kit in Maxwell RSC instrument following manufacturer's instructions. RNA-seq libraries were generated using SENSE mRNA-Seq Library Prep kit V2, following manufacturer's instructions.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-10-15T17:02:11Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-10-04T15:51:43Z
attributes.tag: External Id
attributes.value: SAMEA4952677
attributes.tag: INSDC center name
attributes.value: Department of Molecular Neuroscience, UCL Institute of Neurology, Queen Square, London, WC1N 3BG, UK
attributes.tag: INSDC first public
attributes.value: 2018-10-15T17:02:11Z
attributes.tag: INSDC last update
attributes.value: 2018-10-04T15:51:43Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7271:S2_mRNAseq_siCtrl_2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: S2
attributes.tag: cell type
attributes.value: epithelial cell
attributes.tag: common name
attributes.value: fruit fly
attributes.tag: organism part
attributes.value: late embryo
attributes.tag: sample name
attributes.value: E-MTAB-7271:S2_mRNAseq_siCtrl_2
attributes.tag: scientific_name
attributes.value: Drosophila melanogaster