title: Spacer  acquisition (200_tdI) VvRT-Cas1-Array2
organism: Escherichia coli BL21
description: Targeted DNA sequencing to determine if spliced td  intron RNA is present as cDNA in HMS  174 (DE3) E. coli host over Array 1 over-expressing RT-Cas1, Cas2A+Cas2B - Mix of 200 independent cultures
attributes.tag: isolate
attributes.value: BL21
attributes.tag: host
attributes.value: Escherichia coli
attributes.tag: collection_date
attributes.value: 2019-06-28
attributes.tag: geo_loc_name
attributes.value: Not applicable
attributes.tag: sample_type
attributes.value: cell culture
attributes.tag: altitude
attributes.value: Not applicable
attributes.tag: biomaterial_provider
attributes.value: Not applicable
attributes.tag: collected_by
attributes.value: Estacin Experimental del Zaidn
attributes.tag: culture_collection
attributes.value: Not applicable
attributes.tag: depth
attributes.value: Not applicable
attributes.tag: env_broad_scale
attributes.value: Not applicable
attributes.tag: genotype
attributes.value: Not applicable
attributes.tag: host_tissue_sampled
attributes.value: Not applicable
attributes.tag: identified_by
attributes.value: Not applicable
attributes.tag: lab_host
attributes.value: Escherichia coli BL21
attributes.tag: lat_lon
attributes.value: Not applicable
attributes.tag: mating_type
attributes.value: Not applicable
attributes.tag: passage_history
attributes.value: Not applicable
attributes.tag: serotype
attributes.value: Not applicable
attributes.tag: serovar
attributes.value: Not applicable
attributes.tag: specimen_voucher
attributes.value: Not applicable
attributes.tag: temp
attributes.value: 37
attributes.tag: BioSampleModel
attributes.value: Microbe, viral or environmental