title: HudsonAlpha_MethylRrbs_MCF-7_Randomized_shRNA_control
organism: Homo sapiens
attributes.tag: source_name
attributes.value: MCF-7
attributes.tag: biomaterial provider
attributes.value: ATCC
attributes.tag: lab
attributes.value: HudsonAlpha
attributes.tag: lab description
attributes.value: Myers - Hudson Alpha Institute for Biotechnology
attributes.tag: datatype
attributes.value: MethylRrbs
attributes.tag: datatype description
attributes.value: DNA Methyl Reduced Rep Bisulfite Seq
attributes.tag: cell
attributes.value: MCF-7
attributes.tag: cell organism
attributes.value: human
attributes.tag: cell description
attributes.value: mammary gland, adenocarcinoma. (PMID: 4357757), newly promoted to tier 2: not in 2011 analysis
attributes.tag: cell karyotype
attributes.value: cancer
attributes.tag: cell lineage
attributes.value: ectoderm
attributes.tag: cell sex
attributes.value: F
attributes.tag: treatment
attributes.value: Randomized_shRNA_control
attributes.tag: treatment description
attributes.value: Cells were transduced with a lentivirus expressing an shRNA containing a randomized DNA sequence generated by the siRNA Wizard v3.1 (http://www.sirnawizard.com/scrambled2.php) program. Lentiviral constructs also contained the puromycin resistance selectable marker and cells were treated 2 times, for three days each, to select for cells containing the retroviral construct.
attributes.tag: labexpid
attributes.value: SL14363
attributes.tag: obtainedby
attributes.value: Duke
attributes.tag: obtainedby description
attributes.value: Crawford - Duke University
attributes.tag: replicate
attributes.value: 1