title: activated B cells with Aicda Enhancer 2 deletion (Mus musculus)
organism: Mus musculus
description: Resting splenic B cells were isolated from 6-8 week-old wild-type C57BL6/J mice with anti-CD43 Microbeads (anti-Ly48; Miltenyi Biotech) and were activated for 48-60 h with LPS (50 μg/ml; Sigma), IL-4 (5 ng/ml; Sigma) and 0.5 μg ml−1 of anti-CD180 (RP105) antibody (RP/14, BD Pharmingen).  Aicda enhancer E2 was deleted using TALENs approach.
attributes.tag: age
attributes.value: 6-8 weeks
attributes.tag: cell_type
attributes.value: splenic B cells
attributes.tag: genotype
attributes.value: Aicda enhancer 2 deletion
attributes.tag: description
attributes.value: Switchable IgM+àIgA+ murine CH12-F3 Ly-1+ B cell lymphoma line was maintained and passaged every 2 days in RPMI 1640 supplemented with 10% FBS (ATCC), 1% penicillin/streptomycin (Invitrogen), 55 uM 2-b mercaptoethanol (Invitrogen). All cells were maintained at 37°C and 5% CO2 in a humidified incubator. Aicda enhancer E2 was deleted using TALENs approach.
attributes.tag: cell line
attributes.value: CH12