title: HCT116 siRNF40 TNFa 3
organism: Homo sapiens
description: Protocols: HCT116 were grown in McCoy's medium supplemented with 10% fetal bovine serum, 100 units/ml penicillin and 100 μg/ml streptomycin at 37°C and 5% CO2. siRNA transfections were performed using Lipofectamine® RNAiMAX (Invitrogen) according to the manufacturer's instructions. 48 hours post-transfection, cells were treated with 10 ng/mL of human recombinant TNFα for 30 min. RNA was extracted usiung QIAzol® Lysis Reagent (Qiagen) according to the manufacturer's protocol. Libraries were prepared using TruSeq RNA Library Preparation Kit v2 (Illumina®) after verifying the RNA integrity on an agarose gel. Library quality was assessed using an Agilent Bioanalyzer 2100
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-10-22T17:03:17Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-09-07T16:18:33Z
attributes.tag: External Id
attributes.value: SAMEA4889328
attributes.tag: INSDC center name
attributes.value: University Medical Center Gottingen Clinic for General, Visceral and Pediatric Surgery Tumor Epigenetics
attributes.tag: INSDC first public
attributes.value: 2018-10-22T17:03:17Z
attributes.tag: INSDC last update
attributes.value: 2018-09-07T16:18:33Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7197:HCT116 siRNF40 TNFa 3
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: HCT116
attributes.tag: cell type
attributes.value: epithelial cell
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: colon carcinoma
attributes.tag: organism part
attributes.value: colon
attributes.tag: sample name
attributes.value: E-MTAB-7197:HCT116 siRNF40 TNFa 3
attributes.tag: scientific_name
attributes.value: Homo sapiens