title: Mud loach skin
organism: Misgurnus anguillicaudatus
description: Loach were purchased from a local market in Wuhan, China, and acclimated at 28oC for 5 days. The fish were euthanized with 0.016% tricaine methanesulfonate before tissue collection. Skin of the region between the dorsal fin and lateral line was teared out with scalpel and hemostatic forceps. Total RNA was extracted with TRIZOL Reagent from Invitrogen following the manufacturer’s instructions. Total RNA contents were determined by NanoDrop 8000 from Thermo Scientific. The quality of RNA samples was assessed by agarose gel electrophoresis. Equal volumes of total RNA from three fish were combined and used for RNA-seq analysis.