title: E9.5 Sox17^GFP+EpCAM- cells 3
organism: Mus musculus
description: pool of 26 E9.5 Sox17^GFP/+ embryos Protocols: Embryos were isolated from TgH(Sox17.Cre.GFP)Agb mice; the presence of vaginal plug at noon was considered as E0.5. Embryos were genotyped by fluorescence microscopy. The mid gut areas, including ventral pancreatic buds, were dissected by cutting out on the upper region from the heart and the lower region below the ventral pancreas. The dissection event was confirmed by checking the GFP fluorescence. Tissues were dissociated with Accumax (Sigma) at 37 C. Cells were immuno-labeled with anti-EpCAM (G8.8, Santa Cruz) antibody. Flow Cytometry experiments were performed in the VMC Flow Cytometry Shared Resource on a BD Aria I or II.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2012-07-20T17:00:48Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T15:37:45Z
attributes.tag: External Id
attributes.value: SAMEA1466615
attributes.tag: INSDC center name
attributes.value: Vanderbilt University
attributes.tag: INSDC first public
attributes.value: 2012-07-20T17:00:48Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T15:37:45Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: StrainOrLine
attributes.value: Sox17tm1.3(Cre.GFP)Mgn
attributes.tag: Submitter Id
attributes.value: E-MTAB-970:E9.5 Sox17^GFP+EpCAM- cells 3
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: Sox17 expressing endothelial cell in mid gut
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: E9.5
attributes.tag: sample name
attributes.value: E-MTAB-970:E9.5 Sox17^GFP+EpCAM- cells 3
attributes.tag: scientific_name
attributes.value: Mus musculus