title: Significant changes of community structure of consortia incubated under different conditions for 540 days
organism: oil production facility metagenome
description: This dataset is generated by relatively relaxed criteria (as described below) than those used in the related paper, so as to allow for a complete picture of the data and more data to be preserved for other types of analyses. The primers used for amplification of the 16S rRNA gene V3 region are: the forward primer 5’-NNNNNNNNCCTACGGGAGGCAGCAG-3’ and the reverse primer 5’-NNNNNNNNATTACCGCGGCTGCT-3’. The NNNNNNNN are the sample-unique 8- base barcode. So each read has been labeled with a 8 nucleotide tag adjacent to the primer, however, the first 2 nucleotides are for protection and only the last 6 nucleotides ( 3rd to 8th position of the tag from 5’ to 3’ end ) are used as barcode. All reads from a whole sequencing run are assigned to their corresponding samples as follows: 1) Search the primer with BLAST (Word size=4, e value=0.1) at the sequencing end; 2) Locate the barcode according to the position of the primer; 3) If the barcode is complete, assign the read to the corresponding sample based on the barcode list of the samples of this experiment; 4) If the barcode is not complete, go to the other end to search the primer with BLAST (Word size=4, e value=0.1); 5) Repeat step 2 and 3; 6) If the barcode is not complete, discard the sequence; 7) Repeat this process until all the reads have been checked. Both barcodes and primers are included in the texts of the FASTA file. Barcodes are listed below:  Sample name   barcode  I AGTACATG    LM180 AGTGATAT   HM180 AGTCATCG   LS180 ACATATAG  HS180 AAGAGTAT   LS540 AGCGATGT   LM540 AGATCTCT   HS540 AGATCATG   HM540 AGAGTGCT