title: SF1_OE
organism: Astyanax mexicanus
description: Protocols: Hyphessobrycon anisitsi and Astyanax mexicanus Pachon cave and Texas surface morphs were used. Sample collection included embryos and larvae encompassing stages from 10hpf (hours post-fertilisation) to 17dpf (days post-fertilisation), as well as dissected brains and olfactory epithelia from adults. Samples were immersed in Trizol (Invitrogen) and freezed at -80°C. Total RNA was extracted from the samples according to the manufacturer's instructions for Trizol Reagent (Invitrogen) mRNA were purified from total RNA (FastTrack mRNA isolation kit (Thermo Fischer)), fragmented (NEBNext Magnesium RNA fragmentation module, NEB) and treated with the TruSeq small RNA library preparation Kit (Illumina) according to the manufacturer recommendations. Final libraries were purified with AMPureXP kit (Beckman Coulter) instead of gel purification to recover all fragments. Library quality has been assessed on a bioanalyzer instrument (Agilent).
attributes.tag: ENA first public
attributes.value: 2017-06-13
attributes.tag: ENA last update
attributes.value: 2016-10-11
attributes.tag: External Id
attributes.value: SAMEA4503848
attributes.tag: INSDC center alias
attributes.value: Evolution, Genome, Comportement, Ecologie UMR9191 CNRS - Universite Paris Sud
attributes.tag: INSDC center name
attributes.value: Evolution, Genome, Comportement, Ecologie UMR9191 CNRS - Universite Paris Sud
attributes.tag: INSDC first public
attributes.value: 2017-06-13T17:01:24Z
attributes.tag: INSDC last update
attributes.value: 2016-10-11T15:00:16Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-5142:SF1_OE
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: Mexican tetra
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: organism part
attributes.value: olfactory epithelium
attributes.tag: origin
attributes.value: San Solomon Spring, Texas, USA
attributes.tag: sample name
attributes.value: E-MTAB-5142:SF1_OE
attributes.tag: sampling site
attributes.value: Surface