title: Pool of differentiating barley endosperm transfer cells from grains (3 DAF, 5 DAF, 7 DAF).
organism: Hordeum vulgare subsp. vulgare
description: Differentiating barley ETCs (3 DAF, 5 DAF, 7 DAF) have been isolated from cryosections of barley grains using laser-assisted microdissection. Total RNA was extracted from tissue sections and mRNA was amplified by one round of T7-based amplification. The three antisense (a)RNA populations were pooled in equal amounts and first strand cDNA synthesis was primed with a N6 randomized primer. After ligation of 454 adapters and PCR-amplification (20 cycles), cDNA was normalized and fractioned cDNA in the size range of 400 to 600 nucleotides (gel fractioning) was used for GS FLX Titanium sequencing.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2012-07-20T17:00:48Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T15:39:07Z
attributes.tag: External Id
attributes.value: SAMEA1466837
attributes.tag: INSDC center name
attributes.value: IPK-Gatersleben
attributes.tag: INSDC first public
attributes.value: 2012-07-20T17:00:48Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T15:39:07Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: barley ETCs
attributes.tag: common name
attributes.value: domesticated barley
attributes.tag: sample name
attributes.value: barley ETCs
attributes.tag: scientific_name
attributes.value: Hordeum vulgare subsp. vulgare
attributes.tag: sub_species
attributes.value: vulgare