title: KSL hematopoetic stem cells (Mus musculus)
organism: Mus musculus
description: Hematopoetic stem cells were isolated from the bone marrow of 25 six-week old C57BL6 mice (Jackson Laboratory). Cells were purified following Ema et al.’s protocol (PMID: 17406558). KSL (KIT+, SCA1+, Lin-, IL-7R-) cells were sorted using MoFlo Legacy (Beckman Coulter) and BD FACSAria III.
attributes.tag: age
attributes.value: 6 weeks
attributes.tag: cell_type
attributes.value: hematopoetic stem cells
attributes.tag: description
attributes.value: Hematopoetic stem cells were isolated from the bone marrow of 25 six-week old C57BL6 mice (Jackson Laboratory). Cells were purified following Ema et al.’s protocol (PMID: 17406558). KSL (KIT+, SCA1+, Lin-, IL-7R-) cells were sorted using MoFlo Legacy (Beckman Coulter) and BD FACSAria III.
attributes.tag: breed
attributes.value: C57BL/6