title: Cseq31
organism: Homo sapiens
description: Protocols: MCF10A cells were cultured with DMEM/F12 media supplemented with 2.5% Horse serum, 0.5ug/mL hydrocortisone, 10ug/mL Insulin, 20ng/mL EGF and 100ng/mL cholera toxin. IMR90 cells were cultured in MEM with Earles salts with 10% FBS. We have followed the ChIP protocol previously published in Kim, T.H. et al. Analysis of the vertebrate insulator protein CTCF-binding sites in the human genome. Cell 128, 17382889 (2007). The ChIP DNA was further processed by the Yale Center for Genomic Analysis following the instructions from Illumina, Inc. 35 bases were sequenced.
attributes.tag: ENA first public
attributes.value: 2012-08-01
attributes.tag: ENA last update
attributes.value: 2018-03-08
attributes.tag: External Id
attributes.value: SAMEA1484680
attributes.tag: INSDC center alias
attributes.value: Yale Center for Genomic Analysis
attributes.tag: INSDC center name
attributes.value: Yale Center for Genomic Analysis
attributes.tag: INSDC first public
attributes.value: 2012-08-01T17:00:50Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T15:27:49Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-744:Cseq31
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: MCF 10A
attributes.tag: cell type
attributes.value: epithelial
attributes.tag: common name
attributes.value: human
attributes.tag: sample name
attributes.value: E-MTAB-744:Cseq31