title: KTG_1
organism: Mus musculus
description: Protocols: BALBc and IL4Ra-/- mice were intra-peritoneally injected with 700ul thioglycollate (4%) or intra-peritoneally implanted with 4 B. malayi females and 1 male. Macrophages were harvested 3 days post injection or 21 days post-implantation. Peritoneal exudate cells were harvested by lavage and purified using flow cytometry based upon F4/80 expression. Purity in each sample was confirmed to be >90%. Cells were Qiazol (Qiagen) prior to RNA extraction. Isolation: F4/80+, SiglecF-, B220-, CD4-ve Total RNA was extracted using the miRNeasy kit (Qiagen) according to the manufacturers protocol. Quality was assessed on an Agilent bioanalyzer, all samples had RIN scores >8. Each library was composed of 0.66ug total RNA pooled from 3 mice. RNA-Seq libraries were prepared using the Illumina Paired-End library preparation kit according to the manufacturers instructions.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2012-08-01T17:00:53Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T15:37:50Z
attributes.tag: External Id
attributes.value: SAMEA1486467
attributes.tag: INSDC center name
attributes.value: The University of Edinburgh
attributes.tag: INSDC first public
attributes.value: 2012-08-01T17:00:53Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T15:37:50Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: StrainOrLine
attributes.value: IL4Ra-/-
attributes.tag: Submitter Id
attributes.value: E-MTAB-995:KTG_1
attributes.tag: age
attributes.value: 10 weeks
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: peritoneal macrophage
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: phenotype
attributes.value: KTG
attributes.tag: sample name
attributes.value: E-MTAB-995:KTG_1
attributes.tag: scientific_name
attributes.value: Mus musculus