title: MEFSp2ko_anti-Pbx
organism: Mus musculus
description: Protocols: cell culture, see growth and nucleic acid extraction protocol. Antibodies used: Anti-Pbx1 (Cell Signaling Technology, Danvers, USA, Cat. No. #4342); Anti-Prep1 (Santa Cruz, Dallas, USA, Cat. No. sc-25282); antiSp2 Ab1 (produced in-house) MEFs were cultured in a 1:1 mixture of Dulbecco's modified eagle medium (DMEM)/high glucose (4.5 g/L) (Gibco Thermo Fisher Scientific, Waltham, USA) and HAM's F-10 (Gibco Thermo Fisher Scientific, Waltham, USA) supplemented with 10% (v/v) fetal calf serum (Sigma-Aldrich, St. Louis, USA) and 1% Penicillin-Streptomycin. Sp2ko MEFs expressing Sp2 mutants were selected and propagated in the presence of 2 µg/mL puromycin. Conventional ChIPs of Pbx1, Prep1 and Flag-Sp2 were performed using the One Day ChIP kit (Diagenode, Seraing, Belgium). Three microgram of antibody were used per ChIP experiment, and five nanogram of precipitated DNA were used for indexed sequencing library preparation using the Microplex library preparation kit v2 (Diagenode). Microplex library preparation kit
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-10-17T17:03:27Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-08-09T14:52:33Z
attributes.tag: External Id
attributes.value: SAMEA4824124
attributes.tag: INSDC center name
attributes.value: INSTITUTE FOR MOLECULAR BIOLOGY AND TUMOR RESEARCH
attributes.tag: INSDC first public
attributes.value: 2018-10-17T17:03:27Z
attributes.tag: INSDC last update
attributes.value: 2018-08-09T14:52:33Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7125:MEFSp2ko_anti-Pbx
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: mouse embryonic fibroblast cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: embryo
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: Sp2 homozygous knockout
attributes.tag: organism part
attributes.value: embryo
attributes.tag: sample name
attributes.value: E-MTAB-7125:MEFSp2ko_anti-Pbx
attributes.tag: scientific_name
attributes.value: Mus musculus