title: GluA_T0_2
organism: Corynebacterium glutamicum ATCC 13032
description: Protocols: C. glutamicum GluA T0, GluA T5 and GluA T7 were grown in CGXII minimal medium with 4% (w/v) glucose supplemented with 1 mM IPTG. Exponentially growing cells were harvested by centrifugation (14000 × g, 1 min) and kept at -80°C. C. glutamicum GluA T0, GluA T5 and GluA T7 were grown in CGXII minimal medium with 4% (w/v) glucose supplemented with 1 mM IPTG. Exponentially growing cells were harvested by centrifugation (14000 × g, 1 min) and kept at -80°C. Strain reference: Pérez-García F, Jorge JMP, Dreyszas A, Risse JM, Wendisch VF. Efficient Production of the Dicarboxylic Acid Glutarate by Corynebacterium glutamicum via a Novel Synthetic Pathway. Front Microbiol 2018;9. https://doi.org/10.3389/fmicb.2018.02589. Total RNA was isolated from 3 biological replicates of C. glutamicum cells grown to the exponential phase using a Quick-RNA Miniprep Plus kit according to the manufacturer's instructions (Zymo Research). After additional DNase treatment, RNA samples were purified with an RNA Clean&Concentrator-5 kit (Zymo Research) and quantified with a DropSense 16 (Trinean). The quality of total RNA was controlled with an RNA 6000 Nano kit in an Agilent 2100 Bioanalyzer (Agilent Technologies). To construct the whole transcriptome cDNA library, 2.5 μg total RNA (RIN > 9) was used for the depletion of rRNA with a Ribo-Zero rRNA Removal Kit (Bacteria) according to manufacturer's instructions (Illumina). The rRNA removal was checked with an Agilent RNA Pico 6000 kit and the Agilent 2100 Bioanalyzer (Agilent Technologies). The mRNA obtained was converted to a cDNA library according to the TruSeq Stranded mRNA Sample Preparation guide (Illumina). The quality and quantity of the cDNA library was checked with an Agilent High Sensitivity DNA kit and the Agilent 2100 Bioanalyzer (Agilent Technologies).
attributes.tag: ENA first public
attributes.value: 2021-01-31
attributes.tag: ENA last update
attributes.value: 2021-01-20
attributes.tag: External Id
attributes.value: SAMEA7845129
attributes.tag: INSDC center alias
attributes.value: Technology Platform Genomics, Center for Biotechnology (CeBiTec), Bielefeld University, Sequenz 1, 33615 Bielefeld
attributes.tag: INSDC center name
attributes.value: Technology Platform Genomics, Center for Biotechnology (CeBiTec), Bielefeld University, Sequenz 1, 33615 Bielefeld
attributes.tag: INSDC first public
attributes.value: 2021-01-31T04:08:44Z
attributes.tag: INSDC last update
attributes.value: 2021-01-20T14:13:15Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-10025:GluA_T0_2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: replicate
attributes.value: 2
attributes.tag: sample name
attributes.value: E-MTAB-10025:GluA_T0_2
attributes.tag: strain
attributes.value: parental strain GluA T0 with SNP in gabD from P.stutzerii resulting in amino acid exchange P134L based on published C. glutamicum ATCC13032 modified strain GluA