title: iCLIP_Nova_mm_Brain_20100320_LUjn01_2
organism: Mus musculus
description: Protocols: Mouse brain tissue (Nova iCLIP) or COS-7 cells grown in a tissue culture plate (mutant NSUN2 iCLIP). Tissue was dissociated in ice-cold PBS buffer (Nova iCLIP) or medium was replaced (mutant NSUN2 iCLIP). Material was then subjected to UV-C irradiation. Cells were then precipitated by centrifugation for 1 min at 14000 rpm and snap-frozen on dry ice. iCLIP protocol was followed to isolate RNA bound to Nova protein or mutant NSUN2 protein (C271A) and amplify cDNA library as described in the manuscript. Specific barcodes were used for each RT reaction, and are specified below for each sequencing run. Random barcodes were also incoperated into cDNAs to distinguish between PCR duplication of cDNA products. The final products were obtained by PCR with Illumina pair-end sequencing primers: 5-CAAGCAGAAGACGGCATACGAGATCGGTCTCGGCATTCCTGCTGAACCGCTCTTCCGATCT-3; 5-AATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTTCCGATCT-3
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2012-07-20T17:00:48Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T15:37:17Z
attributes.tag: External Id
attributes.value: SAMEA1466760
attributes.tag: INSDC center name
attributes.value: CRUK-CRI
attributes.tag: INSDC first public
attributes.value: 2012-07-20T17:00:48Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T15:37:17Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-1008:iCLIP_Nova_mm_Brain_20100320_LUjn01_2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: organism part
attributes.value: brain
attributes.tag: sample name
attributes.value: E-MTAB-1008:iCLIP_Nova_mm_Brain_20100320_LUjn01_2
attributes.tag: scientific_name
attributes.value: Mus musculus