title: DNA-seq of Drosophila silvestris adult female replicate 1
organism: Drosophila silvestris
attributes.tag: strain
attributes.value: Y11R6
attributes.tag: age
attributes.value: 4-5 weeks after eclosion (FBdv:00007026)
attributes.tag: dev_stage
attributes.value: adult (FBdv:00007026)
attributes.tag: sex
attributes.value: female
attributes.tag: tissue
attributes.value: whole organism (FBbt:00000001)
attributes.tag: cell_type
attributes.value: not applicable
attributes.tag: growth_protocol
attributes.value: see GSM2113266; 70-100 Flies were grown in gallon glass jars with a 5 cm bed of dry sterile sand. Flies were fed once a week with five vials containing ~3ml of Yeastless Wheeler Clayton medium (placed horizontally in each jar). A single batch of this media was prepared with: 16g each of agar, rice cereal, KelloggÍs Special K, and wheat germ powder, plus 400g GerberÍs banana baby food and 1400ml water. These ingredients were mixed, cooked to boil and cooled. After cooling (but still liquid), 6ml each of 95% ethanol and propionic acid was added and mixed and then used to fill glass dram vials, which were stored 4oC until needed. 1/5 sheet of folded non-bleached facial tissue paper saturated with a water extract of fermenting Clermontia sp. leaves was added to each vial. Females of each strain laid eggs onto the tissue. Vials with eggs were collected weekly. Eggs hatched in about one week. Larvae were fed weekly for 2 weeks (as needed based on density of larvae). The recipe for cornmeal paste larval food was: 800g cornmeal, 100g brewers yeast, 90g soybean meal, 90g agar, 133ml karo syrup, 45g molasses and 4L water. This mixture was boiled, cooled, and 45ml each of 95% ethanol and propionic acid was added. The cornmeal paste is mixed with the water extract of fermenting Clermontia sp. leaves. After 3 weeks the larval tubes were transferred to moist sterile sand jars. Larvae burrow into the sand for pupation. Adults eclosed after 6 weeks and were aged for experiment. All flies were grown at 18oC with a 12h/12h Day/Night cycle.
attributes.tag: BioSampleModel
attributes.value: Model organism or animal