title: Sample 2
organism: Homo sapiens
description: Protocols: 5ug of PolyA+ RNA isolated from the cerebral cortex of post mortem human brain tissue was purchased from Clontech (catalog id 636164). The tissue was pooled from 2 caucasian males, ages: 27 & 78. The cause of death was trauma. RNA was isolated by a modified guanidinium thiocyanate method (Ausubel FM, et al. (1994) Current Protocols in Molecular Biology. (John Wiley & Sons, NY), Section 4) polyA+ RNA selection was performed with two rounds of oligo(dT)-cellulose columns (Sambrook J, et al. (1989) Molecular Cloning: A laboratory Manual, Second Edition (Cold Spring Harbor Laboratory, Cold Spring Harbor, NY), pp. 6.22-6.34) as per manufacturer's instructions, and the sequencing library was prepared using the Illumina TruSeq RNA stranded library preparation kit.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-08-06T17:02:14Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-07-27T11:30:25Z
attributes.tag: External Id
attributes.value: SAMEA4807017
attributes.tag: INSDC center name
attributes.value: UZH
attributes.tag: INSDC first public
attributes.value: 2018-08-06T17:02:14Z
attributes.tag: INSDC last update
attributes.value: 2018-07-27T11:30:25Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7089:Sample 2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: normal
attributes.tag: organism part
attributes.value: cerebral cortex
attributes.tag: sample name
attributes.value: E-MTAB-7089:Sample 2
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: male