title: NoMS-rep3
organism: Verticillium dahliae
description: Protocols: The cultures were of Verticillium dahliae, strain VdLs.17, that was producing (MS) or not producing microsclerotia (NoMS). The NoMS culture could be considered the control. Cultures were grown at 25 C on potato dextrose agar overlaid with a nitrocellulose membrane. All MS and NoMS cultures were ten days old. Total RNA was extracted from the pulverized fungal tissue using a Qiagen RNeasy Kit (Valencia, CA) and following the manufacturer's instructions, and included an on-column DNase I digestion. The sequncing library was constructed by using ScriptSeq_V2 RNA-Seq Library Preparation kit.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2013-09-07
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2013-04-23
attributes.tag: External Id
attributes.value: SAMEA2066308
attributes.tag: INSDC center alias
attributes.value: Univ of Alabama at Birmingham
attributes.tag: INSDC center name
attributes.value: Univ of Alabama at Birmingham
attributes.tag: INSDC first public
attributes.value: 2013-09-07T17:00:44Z
attributes.tag: INSDC last update
attributes.value: 2013-04-23T13:24:45Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-1581:NoMS-rep3
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: phenotype
attributes.value: not microsclerotia-producing
attributes.tag: sample name
attributes.value: E-MTAB-1581:NoMS-rep3
attributes.tag: specimen with known storage state
attributes.value: fresh specimen
attributes.tag: strain
attributes.value: strain VdLs.17