title: s4
organism: Drosophila melanogaster
description: Protocols: Wild-type Drosophila melanogaster (Canton S strain) were raised on standard medium on a 12:12 h L:D cycle, at 23C, and at 55% r.h. To facilitate the collection of accurately staged adults, a laying population of around 12 males and 12 females were transferred to fresh vials daily. When adults subsequently emerged, they were transferred to fresh vials on the day of emergence, and used 7 days later. Organism parts used: tubule (The full length of the Malpighian tubules including upper and lower ureter, severed at their insertion into the hindgut), testes (testes until they end up in the collecting duct), head (The complete head, severed at the neck). RNA extraction: QIAGEN RNeasy Mini columns. 30 wholeflies are pooled for RNA extraction, 100 testes are pooled for RNA extraction, 600pairs of tubules pooled for RNA extraction, 300 heads are pooled fro RNA extraction
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2011-02-08T09:38:24Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T15:50:39Z
attributes.tag: External Id
attributes.value: SAMEA708619
attributes.tag: INSDC center name
attributes.value: SHWFGF
attributes.tag: INSDC first public
attributes.value: 2011-02-08T09:38:24Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T15:50:39Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: StrainOrLine
attributes.value: Canton S
attributes.tag: Submitter Id
attributes.value: E-MTAB-493:s4
attributes.tag: age
attributes.value: 7 days
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: fruit fly
attributes.tag: genotype
attributes.value: wild_type
attributes.tag: organism part
attributes.value: head
attributes.tag: sample name
attributes.value: E-MTAB-493:s4
attributes.tag: scientific_name
attributes.value: Drosophila melanogaster
attributes.tag: sex
attributes.value: mixed_sex