title: LoVo_Xenograft_Control_rep2
organism: Homo sapiens
description: Protocols: Cells for tumour implantation were grown in DMEM supplemented with glutamine and 10% foetal calf serum and implanted subcutaneously in nude mice. ~50mg of tissue were cut from the frozen tumours and RNA isolated using the RNeasy Lipid Tissue Mini Kit (Qiagen) according to manufacturer's instructions. On-column DNase digestion was performed using the RNase-free DNase Kit (Qiagen). Total RNA was cleaned using the Ribominus concentration module (Invitrogen) and rRNA depletion was carried out with the Dynabeads mRNA purification kit (Invitrogen). Libraries enriched with polyA mRNA suitable for sequencing by the SOLiD platform were created using the SOLiD Total RNA Seq Kit supplied by Applied Biosystems in combination with the SOLiD RNA Barcoding Kit, Module 1-16. In each instance, RNase III digested polyA RNA was used as input into library creation and 15 cycles of amplification were employed to produce the final libraries.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2013-09-09T17:00:31Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T16:23:06Z
attributes.tag: External Id
attributes.value: SAMEA2065414
attributes.tag: INSDC center name
attributes.value: AZ
attributes.tag: INSDC first public
attributes.value: 2013-09-09T17:00:31Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T16:23:06Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-1627:LoVo_Xenograft_Control_rep2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: LoVo
attributes.tag: common name
attributes.value: human
attributes.tag: sample name
attributes.value: E-MTAB-1627:LoVo_Xenograft_Control_rep2
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: specimen with known storage state
attributes.value: frozen sample