title: 8_A6_KIvCFj7HdT3X
organism: Drosophila melanogaster
description: Protocols: All fly stocks were grown at 25 C and a 12h light-dark cycle on corn-meal fly medium. We collected virgin flies from three of the DGRP lines (lines 362, 517 and 765) during three days and we set up the following crosses: _ 362 x _ 517 (and reciprocal) and _ 517 x _ 765 (and reciprocal). We performed RNA-seq to assess transcript expression profiles of 3-5 day old F1 females. 25 flies per sample were frozen between 1-3 pm Total RNA was extracted using the combined Trizol/RNeasy (Qiagen, www1.Qiagen.com) protocol. RNA quality was measured using RNA Labchips and Bioanalyzer from Agilent Technologies (http://www.chem.agilent.com). RNA-seq libraries were prepared using the RNA-True seq kit (Illumina).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2012-08-28T17:00:46Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T15:55:35Z
attributes.tag: External Id
attributes.value: SAMEA1487544
attributes.tag: INSDC center name
attributes.value: EPFL
attributes.tag: INSDC first public
attributes.value: 2012-08-28T17:00:46Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T15:55:35Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: StrainOrLine
attributes.value: DGRP-517/765
attributes.tag: Submitter Id
attributes.value: E-MTAB-1266:8_A6_KIvCFj7HdT3X
attributes.tag: age
attributes.value: 3 to 5 day
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: fruit fly
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: sample name
attributes.value: E-MTAB-1266:8_A6_KIvCFj7HdT3X
attributes.tag: scientific_name
attributes.value: Drosophila melanogaster
attributes.tag: sex
attributes.value: female