title: Desiree_PVY_DSN
organism: Solanum tuberosum
description: Potato plants  (Solanum tuberosum L.) were grown in stem node tissue culture. Two weeks  after node segmentation, plants were transferred to soil and kept in growth  chambers under controlled environmental conditions at 22/20 C with a long-day (16-h) photoperiod  of light (light intensity 4000 lm/m2) and 60%-70% relative humidity. Leaves  were inoculated with PVY(NTN). Inoculated leaves were sampled 3 days post  inoculation (3 dpi), frozen immediately in liquid nitrogen, and stored at -80  C. Frozen leaf samples were homogenized using a TissueLyzer (Qiagen, Germany)  and total RNA was extracted using RNeasy Plant Mini kits (Qiagen), treated  with DNase I (Invitrogen), and cleaned using RNeasy MinElute Cleanup Kit (Qiagen).
attributes.tag: cultivar
attributes.value: Desiree
attributes.tag: age
attributes.value: 4 weeks
attributes.tag: geo_loc_name
attributes.value: not applicable
attributes.tag: tissue
attributes.value: leaf
attributes.tag: treatment
attributes.value: PVY
attributes.tag: ry(sto)_carrier
attributes.value: no
attributes.tag: BioSampleModel
attributes.value: Plant