title: Bacteria
organism: Bacteria
description: Animal 3 control (no yeast supplementation)
attributes.tag: Animal
attributes.value: Holstein cows
attributes.tag: Sample type
attributes.value: Rumen
attributes.tag: Whole content
attributes.value: 500mL
attributes.tag: Storage
attributes.value: -80
attributes.tag: Preparation
attributes.value: Freeze-dried
attributes.tag: Amount used
attributes.value: 100
attributes.tag: Extraction method
attributes.value: Qiagen Stool Kit
attributes.tag: Primer combination
attributes.value: 27F(adaptB)-357R(adaptA)
attributes.tag: Number of cycles
attributes.value: 30
attributes.tag: Purification
attributes.value: Montage™ PCR96 Cleanup
attributes.tag: Qualtity and quantity
attributes.value: Biorad Experion
attributes.tag: Sequencing primer for unidirectional read
attributes.value: 357R(adaptA)
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2012-08-30
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08