title: Phased, diploid assembly of Cascade hop (Humulus lupulus)
organism: Humulus lupulus var. lupulus
description: Hop (Humulus lupulus L. var Lupulus) is a diploid, dioecious plant valued for its use in brewing and traditional medicine. The hop genome is large, repetitive, and heterozygous, which challenged early attempts at sequencing with short-reads. Presented here is the long-read, haplotype-phased assembly of the hop cultivar Cascade. A haplotype-phased assembly allows for investigation of structural variation between haplotypes and improved resolution of gene copy variation. Sequencing was done with PacBio long-read sequencing, assembly was performed with FALCON, and phasing with FALCON-Unzip.
attributes.tag: cultivar
attributes.value: Cascade
attributes.tag: collection_date
attributes.value: 2016-12/2017-02
attributes.tag: env_broad_scale
attributes.value: greenhouse under controlled conditions
attributes.tag: env_local_scale
attributes.value: N/A
attributes.tag: env_medium
attributes.value: N/A
attributes.tag: geo_loc_name
attributes.value: USA:Oregon
attributes.tag: host
attributes.value: N/A
attributes.tag: isol_growth_condt
attributes.value: Grown under controlled conditions in a greenhouse setting and sprayed for insect pests and plant diseases.
attributes.tag: lat_lon
attributes.value: N/A
attributes.tag: variety
attributes.value: lupulus
attributes.tag: BioSampleModel
attributes.value: MIGS.eu
attributes.tag: BioSampleModel
attributes.value: MIGS/MIMS/MIMARKS.plant-associated