title: WT_3
organism: Mus musculus
description: Protocols: Peripheral lymph nodes (LN) were minced and digested at 37 degrees C in RPMI (PAA) containing 0.25 mg/ml Collagenase D (Roche), 1 U/ml Dispase I (Roche), 25 mM Hepes, pH 7.2, and 120 U/ml DNase I (Roche) for 40 min, followed by addition of 5 mM EDTA and incubation for 5 min. LNs were digested with 0.5U/ml Liberase TM (Roche) and DNase I. Aire-ILC3s were sorted as Lin-, MHCII+, CD80+, IL7Ra+ cells on INFLUX sorter (BD). Total mRNA was extracted from the FACS-sorted cells using RNeasy Plus Microkit (Qiagen). The quality of the isolated RNA was controlled by Bioanalyzer 2100 electrophoresis (Agilent). RNA-seq libraries were prepared by EMBL Gene core facility in Heidelberg.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-03-14T17:02:42Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-07-27T11:51:35Z
attributes.tag: External Id
attributes.value: SAMEA4807023
attributes.tag: INSDC center name
attributes.value: Laboratory of Genomics and Bioinformatics Institute of Molecular Genetics of the ASCR
attributes.tag: INSDC first public
attributes.value: 2019-03-14T17:02:42Z
attributes.tag: INSDC last update
attributes.value: 2018-07-27T11:51:35Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7088:WT_3
attributes.tag: age
attributes.value: 4 to 6
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: group 3 innate lymphoid cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: lymph node
attributes.tag: phenotype
attributes.value: Lin-, MHCII+, CD80+, IL7Ra+
attributes.tag: sample name
attributes.value: E-MTAB-7088:WT_3
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: BALB/c