title: Sample 23
organism: Homo sapiens
description: Protocols: Human mesenchymal stem cells were treated with and without cyclic tensile strain (2.6 - 6.2% strain, 5.0 Hz, 1 hour) and incubated at 37°C, 5% CO2 for either 0 or 24 hrs. Following incubation, cells were washed with PBS, dissociated using trypsin EDTA, pelleted and frozen at -80°C for future use. RNA was extracted from cell pellets using the RNeasy Mini kit (Qiagen) and an on-column DNA digest performed using the RNase-Free DNase kit (Qiagen). The RNA was eluted in water and the quality and quantity assessed using a NanoDrop ND-1000 spectrometer (Thermo Fisher). TruSeq® Stranded mRNA assay (Illumina, Inc.) according to the manufacturer's protocol
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-06-18T17:08:10Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-04-30T16:33:27Z
attributes.tag: External Id
attributes.value: SAMEA5585748
attributes.tag: INSDC center name
attributes.value: UNIVERSITY OF MANCHESTER
attributes.tag: INSDC first public
attributes.value: 2019-06-18T17:08:10Z
attributes.tag: INSDC last update
attributes.value: 2019-04-30T16:33:27Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7925:Sample 23
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: mesenchymal stem cell
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: individual
attributes.value: 74
attributes.tag: organism part
attributes.value: bone marrow
attributes.tag: sample name
attributes.value: E-MTAB-7925:Sample 23
attributes.tag: scientific_name
attributes.value: Homo sapiens