title: PW363_Rysto_PVY_DSN
organism: Solanum tuberosum
description: Potato plants (Solanum tuberosum L.) were  grown in stem node tissue culture. Two weeks after node segmentation, plants  were transferred to soil and kept in growth chambers under controlled  environmental conditions at 22/20 C  with a long-day (16-h) photoperiod of light (light intensity 4000 lm/m2) and  60%-70% relative humidity. Leaves were inoculated with PVY(Wilga). Inoculated  leaves were sampled 3 days post inoculation (3 dpi), frozen immediately in  liquid nitrogen, and stored at -80 C. Frozen leaf samples were homogenized  using a TissueLyzer (Qiagen, Germany) and total RNA was extracted using  RNeasy Plant Mini kits (Qiagen), treated with DNase I (Invitrogen), and  cleaned using RNeasy MinElute Cleanup Kit (Qiagen).
attributes.tag: cultivar
attributes.value: PW363
attributes.tag: age
attributes.value: 4 weeks
attributes.tag: geo_loc_name
attributes.value: not applicable
attributes.tag: tissue
attributes.value: leaf
attributes.tag: treatment
attributes.value: PVY
attributes.tag: ry(sto)_carrier
attributes.value: yes
attributes.tag: BioSampleModel
attributes.value: Plant