title: Stanford_ChipSeq_A549_CTCF_(SC-15914)_IgG-rab
organism: Homo sapiens
attributes.tag: source_name
attributes.value: A549
attributes.tag: biomaterial_provider
attributes.value: ATCC
attributes.tag: lab
attributes.value: Stanford
attributes.tag: lab description
attributes.value: Snyder - Stanford University
attributes.tag: datatype
attributes.value: ChipSeq
attributes.tag: datatype description
attributes.value: Chromatin IP Sequencing
attributes.tag: cell
attributes.value: A549
attributes.tag: cell organism
attributes.value: human
attributes.tag: cell description
attributes.value: epithelial cell line derived from a lung carcinoma tissue. (PMID: 175022), "This line was initiated in 1972 by D.J. Giard, et al. through explant culture of lung carcinomatous tissue from a 58-year-old caucasian male." - ATCC, newly promoted to tier 2: not in 2011 analysis
attributes.tag: cell karyotype
attributes.value: cancer
attributes.tag: cell lineage
attributes.value: endoderm
attributes.tag: cell sex
attributes.value: M
attributes.tag: treatment
attributes.value: None
attributes.tag: treatment description
attributes.value: No special treatment or protocol applies
attributes.tag: antibody
attributes.value: CTCF_(SC-15914)
attributes.tag: antibody antibodydescription
attributes.value: Goat polyclonal, epitope mapping near the C-terminus of CTCF of human origin. Antibody Target: CTCF
attributes.tag: antibody targetdescription
attributes.value: Transcriptional regulator with 11 highly conserved zinc finger domains. Depending on context, can bind a histone acetyltransferase (HAT)-containing complex and function as transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as transcriptional repressor. Involved in transcriptional regulation by binding to chromatin insulators and preventing interaction between promoter and nearby enhancers and silencers. Preferentially interacts with unmethylated DNA, preventing spreading of CpG methylation. Can dimerize, mediating long-range chromatin looping. When bound to chromatin, provides an anchor point for nucleosomes positioning. Involved in sister chromatid cohesion. Associates with both centromeres and chromosomal arms during metaphase and required for cohesin localization to CTCF sites.
attributes.tag: antibody vendorname
attributes.value: Santa Cruz Biotech
attributes.tag: antibody vendorid
attributes.value: sc-15914
attributes.tag: control
attributes.value: IgG-rab
attributes.tag: control description
attributes.value: Input signal from Normal Rabbit IgG ChIP-seq.
attributes.tag: control
attributes.value: IgG-rab
attributes.tag: control description
attributes.value: Input signal from Normal Rabbit IgG ChIP-seq.
attributes.tag: controlid
attributes.value: wgEncodeEH002819