title: caRNA fraction2
organism: Drosophila melanogaster
description: Protocols: Chromatin assembly and accessibility analysis were performed as described elsewhere (Becker and Wu, 1992; Rhodes and Laskey, 1989; Stewart et al., 1991) For RNA isolation, chromatin was fractionated in a linear sucrose gradient (15-40%; 12 h; 7500g; 4�C). Fractions containing the chromatin associated RNA were de-proteinized and treated with DNase I. Isolated RNA was enriched for fragments < 200 bp and strand-specific cDNA libraries were prepared using the ExactStart Small RNA Cloning Kit (Epicentre). Libraries included small coding as well as non-coding RNAs and fragments of longer primary transcripts. The cDNA was amplified with hybrid primers containing sequences required for the Illumina platform. PCR products were cleaned up with AMPure XP beads (Beckman Coulter).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2014-06-19T17:01:49Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T18:27:05Z
attributes.tag: External Id
attributes.value: SAMEA2604744
attributes.tag: INSDC center name
attributes.value: University of Regensburg
attributes.tag: INSDC first public
attributes.value: 2014-06-19T17:01:49Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T18:27:05Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-1237:caRNA fraction2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: embryonal extract
attributes.tag: common name
attributes.value: fruit fly
attributes.tag: developmental stage
attributes.value: embryo
attributes.tag: sample name
attributes.value: E-MTAB-1237:caRNA fraction2
attributes.tag: scientific_name
attributes.value: Drosophila melanogaster