title: UntransplantedNSG_2_20026
organism: Mus musculus
description: Protocols: humanCD45 enriched xenograft samples from NSG or NSG-S xentransplanted mice with JMML CD34+ cells. For secondary xenografts, bone marrow cells from primary engrafted mice were injected in another mouse to test the self-renewal capacities of JMML cells. The bone marrow cells of the primary mice were not sorted before the injection into the secondary recipient because the percentage of human CD45 was high. Qiagen Mini or Midi Kit (Qiagen Gmbh) Exome capture was carried out using the SureSelect Human All Exon V5 or SureSelect Clinical Research (Agilent Technologies, Santa Clara, CA, USA) according to manufacturer's instruction and protocols.
attributes.tag: ENA first public
attributes.value: 2018-03-15
attributes.tag: ENA last update
attributes.value: 2018-02-06
attributes.tag: External Id
attributes.value: SAMEA104569302
attributes.tag: INSDC center alias
attributes.value: Assistance Publique des Hopitaux de Paris (AP-HP), Hopital Robert Debre, Departement de Genetique, Paris, France
attributes.tag: INSDC center name
attributes.value: Assistance Publique des Hopitaux de Paris (AP-HP), Hopital Robert Debre, Departement de Genetique, Paris, France
attributes.tag: INSDC first public
attributes.value: 2018-03-15T17:01:43Z
attributes.tag: INSDC last update
attributes.value: 2018-02-06T16:19:59Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6467:UntransplantedNSG_2_20026
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: mononuclear cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: disease
attributes.value: diabetes and severe combined immunodeficiency
attributes.tag: individual
attributes.value: NSG mouse 2
attributes.tag: phenotype
attributes.value: human CD45 positive
attributes.tag: sample name
attributes.value: E-MTAB-6467:UntransplantedNSG_2_20026