title: 2IFN-
organism: mixed sample
description: Protocols: Fresh water without nitrogen Samples for RNA isolation were immediately processed after sampling and kept on ice. Cells were collected by centrifugation at 4.000xg at 0°C for three minutes and immediately frozen in liquid nitrogen before storage at -80°C until further extraction. Cells were disrupted by grinding the pellet using mortar and pestle and liquid nitrogen. A 5 ml volume of heated (65°C) phenol-chloroform and 5 ml of extraction buffer (10 mM EDTA, 1% sodium dodecyl sulphate (SDS), 2% 2-mercaptoethaol and 200 mM sodium acetate, pH 5) was added to the ground biomass. The RNA was precipitated by addition of 1/3 volume 8M lithium chloride (LiCl) enriched with 1% 2-mercaptoethanol. The RNA pellet was washed with 2M LiCl and twice with 70% ethanol. After evaporation of the last residues of ethanol, the pellet was resuspended in RNase free H2O. Illumina TruSeq RNA protocol
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-03-14T17:02:44Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-09T13:27:41Z
attributes.tag: External Id
attributes.value: SAMEA3484720
attributes.tag: INSDC center name
attributes.value: Wageningen University, Laboratory of systems and synthetic biology.
attributes.tag: INSDC first public
attributes.value: 2018-03-14T17:02:44Z
attributes.tag: INSDC last update
attributes.value: 2018-03-09T13:27:41Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-3746:2IFN-
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: growth condition
attributes.value: Fresh water, nitrogen deplete
attributes.tag: sample name
attributes.value: E-MTAB-3746:2IFN-
attributes.tag: scientific_name
attributes.value: Ettlia oleoabundans
attributes.tag: component_organism
attributes.value: Ettlia oleoabundans
attributes.tag: component_organism
attributes.value: Ettlia oleoabundans