title: patient92-12561
organism: Homo sapiens
description: Protocols: The study included 15 patients (7 males, 8 females) with JMML. Peripheral blood and/or bone marrow aspirates were collected on EDTA at diagnosis. Non-hematopoietic tissues (fibroblasts) was derived from skin biopsy for each patient xhen available Genomic DNA was extracted from mononuclear cells and fibroblasts using a Qiagen Mini or Midi Kit (Qiagen Gmbh). RNA was extracted from mononuclear cells using the miRNeasy Mini Kit (Qiagen). Concentrations were measured using a NanoDrop® (Thermo Scientific). Targeted enrichment and massive parallel sequencing were performed on paired genomic DNA from leukocytes and fibroblasts. Exome capture was carried out using the SureSelect Human All Exon V4+UTRs or V5 or V5+UTRs or SureSelect Clinical Research (Agilent Technologies, Santa Clara, CA, USA) according to manufacturer's instruction and protocols.
attributes.tag: ENA first public
attributes.value: 2018-03-14
attributes.tag: ENA last update
attributes.value: 2018-02-08
attributes.tag: External Id
attributes.value: SAMEA104570358
attributes.tag: INSDC center alias
attributes.value: Assistance Publique des Hopitaux de Paris (AP-HP), Hopital Robert Debre, Departement de Genetique, Paris, France
attributes.tag: INSDC center name
attributes.value: Assistance Publique des Hopitaux de Paris (AP-HP), Hopital Robert Debre, Departement de Genetique, Paris, France
attributes.tag: INSDC first public
attributes.value: 2018-03-14T17:02:01Z
attributes.tag: INSDC last update
attributes.value: 2018-02-08T10:16:09Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6461:patient92-12561
attributes.tag: age
attributes.value: 4.5
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: mononuclear cell
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: juvenile myelomonocytic leukemia
attributes.tag: disease staging
attributes.value: diagnosis
attributes.tag: genotype
attributes.value: somatic genotype
attributes.tag: individual
attributes.value: patient92
attributes.tag: organism part
attributes.value: bone marrow cell
attributes.tag: sample name
attributes.value: E-MTAB-6461:patient92-12561
attributes.tag: sex
attributes.value: female