title: Col-0_2
organism: Arabidopsis thaliana
description: Protocols: Seedlings were grown on half-strength (0.5×) Murashige and Skoog (MS) medium under the growth chamber conditions 16 h day/8 h night with white light and 20°C. 10-day old Arabidopsis seedlings at the developmental stage 1.02 (Boyes et al., 2001) were dissected with laboratory micro scissors to remove roots and cotyledons and obtain shoot apices. The Arabidopsis seeds were sterilized in 5% (v/v) bleach containing 0.05% (v/v) Tween 20 for 10 min, washed in water, sown on half-strength Murashige Skoog (MS) medium and stratified at 4°C for 48 h. Subsequently seedlings were grown under the growth chamber conditions 16 h day/8 h night with white light and 20°C. The poly-A containing mRNA molecules were selected and converted to cDNA using the reagents provided in the Illumina TruSeq Stranded mRNA Sample Preparation Kits. The dissected shoot apices were frozen in liquid nitrogen and homogenized. RNA was isolated with RNeasy Plant Kit (Qiagen) with on-column DNase digestion. The manufacturer's protocol was modified by including two additional washes of RNeasy spin columns with the RPE buffer. The library of cDNA template molecules of known strand origin was obtained using the reagents provided in the Illumina TruSeq Stranded mRNA Sample Preparation Kits.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-05-14T17:05:50Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-05-11T15:55:43Z
attributes.tag: External Id
attributes.value: SAMEA4659714
attributes.tag: INSDC center name
attributes.value: Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, Belgium VIB Center for Plant Systems Biology,VIB, Gent, Belgium
attributes.tag: INSDC first public
attributes.value: 2018-05-14T17:05:50Z
attributes.tag: INSDC last update
attributes.value: 2018-05-11T15:55:43Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6780:Col-0_2
attributes.tag: age
attributes.value: 10
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: thale cress
attributes.tag: developmental stage
attributes.value: Stage 1.02, two rosette leaves >1 mm in length
attributes.tag: ecotype
attributes.value: Col-0
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: shoot apex
attributes.tag: sample name
attributes.value: E-MTAB-6780:Col-0_2
attributes.tag: scientific_name
attributes.value: Arabidopsis thaliana