title: Brewery Waste MEC, Day 108-supernatant
organism: bioreactor metagenome
description: Active microbial population within supernatant of brewery waste MEC. Sample was collected on day 108, and RNA was extracted from cells. RNA was randomly reverse transcribed, then PCR was performed with A109f-U529r primers (Archaeal, V2-V3) and with B27f-U529r (Bacterial, V1-V3) to create 16S rRNA amplicons. These were sequenced with PacBio, and only circular consensus reads were used.