title: Sample 3
organism: Homo sapiens
description: Protocols: Cells were transduced with non-targeting and TIGAR-targeting shRNAs for 54 hours. Scrambled non-targeting shRNA was used as a control. Cells were cultured in DMEM with 10% FBS. Cells were transduced with TIGAR shRNA or non-targeting shRNA viral supernatant. Trizol method was used to extract total RNA 1 ug of total RNA was used to synthesize cDNA, and library was prepared using Illumina TruSeq RNA Sample Preparation Kit v2.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-10-24T17:02:19Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-10-10T11:25:17Z
attributes.tag: External Id
attributes.value: SAMEA4968314
attributes.tag: INSDC center name
attributes.value: UNIVERSITY OF NEW MEXICO
attributes.tag: INSDC first public
attributes.value: 2018-10-24T17:02:19Z
attributes.tag: INSDC last update
attributes.value: 2018-10-10T11:25:17Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7284:Sample 3
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: OVCA420
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: ovarian serous adenocarcinoma
attributes.tag: genotype
attributes.value: scrambled_shRNA
attributes.tag: organism part
attributes.value: ovary
attributes.tag: phenotype
attributes.value: wild type
attributes.tag: rna interference
attributes.value: scrambled siRNA
attributes.tag: sample name
attributes.value: E-MTAB-7284:Sample 3
attributes.tag: scientific_name
attributes.value: Homo sapiens