title: Phomopsis longicolla isolate (MS10-6)
organism: Diaporthe longicolla MSPL 10-6
description: The Phomopsis longicolla isolate (MS10-6) was isolated from the seed harvested in the field plots at Stoneville in Mississippi. Briefly, Seeds were surface-disinfected in 0.5% sodium hypochlorite for 3 min, rinsed in sterile distilled water, and then placed on potato dextrose agar (Difico Laboratories, Detroit, MI) that was acidified (pH 4.8) with 25% lactic acid (APDA). After 4 d of incubation at 24*C, the fungus was isolated and transferred to a new culture plate. Identification of P. longicolla was based on morphological characteristics according to Hobbs et al., (1985) and confirmed by the sequence of the ribosomal internal transcribed spacers.
attributes.tag: country
attributes.value: USA: Stoneville, MS
attributes.tag: isolation-source
attributes.value: seed harvested in the field plots
attributes.tag: strain
attributes.value: MSPL 10-6