title: Glomus diaphanum DAOM 229456
organism: Oehlia diaphana
description: This read set was one of four that was used to as a basis for the evolutionary network analysis in "Estimating genome diversity in arbuscular mycorrhizal fungi: the case for a pangenome" by Eva Boon, S*bastien Halary, Eric Bapteste and Mohamed Hijri  The fungal material of isolate Glomus diaphanum DAOM 229456 was obtained from in vitro cultures with Agrobacterium rhizogenens transformed carrot roots. Spores and hyphae were freshly harvested by dissolving the Gellan-Gum matrix in which cultures were grown in a solution containing 0.0083 N sodium citrate and 0.0017 N citric acid. Spores and hyphae were gently crushed in a 1.5 ml microtube using a sterilized pestle. DNA was extracted using DNeasy Plant Mini kit (Qiagen), according to manufacturer's instructions. The purified DNA was then sent to the Genome Qu*bec Innovation Centre (McGill University, Montr*al) for pyrosequencing using the GS FLX Titanium whole genome shotgun kit (Roche 454 Life Science), employing a full run for each DNA sample