title: 14 days seedling of Caragana Intermedia Sample
organism: Caragana Intermedia
description: Germplasm resources collection and plant materials treatments  Seeds of C. intermedia used in the present study were collected from pods of C. intermedia which growing in desert habitat in Inner Mongolia, China. The pods splits into two valves with the seeds attached to one edge of the valves. Seeds were washed five times with tap water, and then germinated in sand. Two weeks later, the young seedlings were selected and prepared for the total RNA isolation. The entire 14 days seedling of C. intermedia plant tissues were snap-frozen and ground under liquid nitrogen before RNA extraction.