title: Festuca pratensis ‘B14/16’, cold acclimated sample
organism: Festuca pratensis
description: Festuca pratensis ‘B14/16’, cold acclimated sample Three clonal plants were grown at 20/12*C day/night, 16/8h light/darkness with light intensity 240 μmolm-2s-1 for two weeks and then subjected to cold acclimation at 1*C. Total RNA (100mg) was isolated from crown, stem and leaf tissues at 1, 2 and 3 weeks after initiation of cold acclimation using the RNeasy Mini kit (Qiagen, Hilden, Germany). cDNA was synthesized using MINT-Universal cDNA synthesis kit (Evrogen, protocol-II) and sequenced using a single-end sequencing protocol on the Roche 454 Titanium sequencing machine.