title: Sample 6
organism: Populus tremula x Populus alba
description: Protocols: Tissues of developing and mature xylem were taken seperately and directly frozen in liquid nitrogen. Plants were multiplied by in vitro propagation and kept on 1/2 MS-medium for four weeks. Afterwards, plants were planted in clay pots filled with a soil-sand-mixture. Poplars were kept in climate chambers for 8 weeks under long-day conditions (16 hours daylength) at 200 µmol*m-2*s-1 PAR at 22 °C and 60% humidity. The treatment of three month old poplars took place for five weeks in climate chambers. First, half of the plants were kept under long-day (16 h) or short-day (8 h) conditions. After two weeks, half of each daylength treated group was additionally drought stress treated (0.1-0.2 m³ water/m³ soil) while the others were still well-watered. Nucleic acid was extracted according to CTAB extraction protocol (Chang et al., 1993). TruSeq mRNA Sample Prep kit v2 (Illumina, San Diego, CA, USA) according to manufacturer's instructions.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-10-21T17:02:23Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-10-11T15:35:00Z
attributes.tag: External Id
attributes.value: SAMEA4969676
attributes.tag: INSDC center name
attributes.value: Georg-August-Universitat Busgen-Institut Department of Forest Botany and Tree Physiology
attributes.tag: INSDC first public
attributes.value: 2018-10-21T17:02:23Z
attributes.tag: INSDC last update
attributes.value: 2018-10-11T15:35:00Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7288:Sample 6
attributes.tag: age
attributes.value: 13
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cultivar
attributes.value: INRA717 1-B4
attributes.tag: developmental stage
attributes.value: sapling
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: growth condition
attributes.value: long day length regimen
attributes.tag: organism part
attributes.value: xylem
attributes.tag: sample name
attributes.value: E-MTAB-7288:Sample 6
attributes.tag: scientific_name
attributes.value: Populus tremula x Populus alba