title: PE_109
organism: Mus musculus
description: Protocols: Adult mice were treated with AngII, PE, or vehicle for 3 days using in vivo mini pumps. Hearts were excised and myocytes were isolated. Total RNA was collected from isolated myocytes. Total RNA was extracted from isolated myocytes using RNeasy Fibrous Mini Tissue Kit (Qiagen) Total RNA was used as input, and oligo-dT purification to enrich for mRNA, followed by random-primed reverse-transcription, second-strand cDNA synthesis, ligation of indexed adaptors for Illumina sequencing, and subsequent library creation from the resulting double-stranded DNA.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-05-12T17:02:50Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-05-08T09:26:52Z
attributes.tag: External Id
attributes.value: SAMEA4647080
attributes.tag: INSDC center name
attributes.value: UMENT
attributes.tag: INSDC first public
attributes.value: 2018-05-12T17:02:50Z
attributes.tag: INSDC last update
attributes.value: 2018-05-08T09:26:52Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6770:PE_109
attributes.tag: age
attributes.value: 10 to 11
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: myocyte
attributes.tag: common name
attributes.value: house mouse
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: heart
attributes.tag: sample name
attributes.value: E-MTAB-6770:PE_109
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: FVB/NJ