title: HRS
organism: Panonychus citri
description: Protocols:A colony of Citrus red mite, Panonychus citri (McGregor), was collected from citrange next to citrus orchard fences,which had never been exposed to acaricides for many years. These mites were reared in citrange for 4 years under acaricide-free conditions (25 +/-1 degree C, 80 +/-5% RH and photoperiod of 14 hours light: 10 hours darkness) and considered as a susceptible strain.The susceptible strain was treated with hexythiazox (about 70% population mortality rate in every spraying) and continuously screened for 28 generations for 4 years, and the resultant strain was regarded as the resistant strain.The mixture of eggs, larvae and adults was collected in a PE (PerkinElmer) tube as one sample for SS and RS, respectively. A collection of 200 individuals was used for each of the sample (SS and RS) for total RNA extraction
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2012-09-07T17:02:24Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T15:52:48Z
attributes.tag: External Id
attributes.value: SAMEA1501589
attributes.tag: INSDC center name
attributes.value: BGI
attributes.tag: INSDC first public
attributes.value: 2012-09-07T17:02:24Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T15:52:48Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: StrainOrLine
attributes.value: hexythiazox resistant strains
attributes.tag: Submitter Id
attributes.value: E-MTAB-1193:HRS
attributes.tag: age
attributes.value: 4 year
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: citrus red mite
attributes.tag: compound
attributes.value: hexythiazox
attributes.tag: dose
attributes.value: 233.1 microgram per liter
attributes.tag: sample name
attributes.value: E-MTAB-1193:HRS
attributes.tag: scientific_name
attributes.value: Panonychus citri
attributes.tag: sex
attributes.value: mixed sex