title: HudsonAlpha_ChipSeq_A549_USF-1_EtOH_0.02pct_v042211.1
organism: Homo sapiens
attributes.tag: source_name
attributes.value: A549
attributes.tag: biomaterial_provider
attributes.value: ATCC
attributes.tag: datatype
attributes.value: ChipSeq
attributes.tag: datatype description
attributes.value: Chromatin IP Sequencing
attributes.tag: antibody antibodydescription
attributes.value: Rabbit polyclonal, USF-1 (C-20). Antibody Target: USF1
attributes.tag: antibody targetdescription
attributes.value: The ubiquitously expressed cellular upstream stimulatory factor (USF) consists of USF-1 and USF-2 polypeptides which independently exhibit site-specific DNA binding and are members of the c-Myc-related family of regulatory factors containing helix-loop-helix domains. USF also contains a leucine repeat that is required for efficient DNA binding. USF was originally identified as an up- stream stimulatory factor that binds the core sequence CACGTG in the adeno- virus late promoter. These findings, together with the demonstration of coop- erative interaction between USF and the initiator-binding protein, TFII-I, raises the possibility of a more general involvement of USF in transcriptional regula- tion. While expression of both USF-1 and USF-2 species is ubiquitous, different ratios of USF homo- and heterodimers are found in different cell types.
attributes.tag: antibody vendorname
attributes.value: Santa Cruz Biotechnology
attributes.tag: antibody vendorid
attributes.value: sc-229
attributes.tag: controlid
attributes.value: SL6021
attributes.tag: labexpid
attributes.value: SL7279,SL6593
attributes.tag: softwareversion
attributes.value: MACS
attributes.tag: cell sex
attributes.value: M
attributes.tag: antibody
attributes.value: USF-1
attributes.tag: antibody antibodydescription
attributes.value: Rabbit polyclonal, USF-1 (C-20). Antibody Target: USF1
attributes.tag: antibody targetdescription
attributes.value: The ubiquitously expressed cellular upstream stimulatory factor (USF) consists of USF-1 and USF-2 polypeptides which independently exhibit site-specific DNA binding and are members of the c-Myc-related family of regulatory factors containing helix-loop-helix domains. USF also contains a leucine repeat that is required for efficient DNA binding. USF was originally identified as an up- stream stimulatory factor that binds the core sequence CACGTG in the adeno- virus late promoter. These findings, together with the demonstration of coop- erative interaction between USF and the initiator-binding protein, TFII-I, raises the possibility of a more general involvement of USF in transcriptional regula- tion. While expression of both USF-1 and USF-2 species is ubiquitous, different ratios of USF homo- and heterodimers are found in different cell types.
attributes.tag: antibody vendorname
attributes.value: Santa Cruz Biotechnology
attributes.tag: antibody vendorid
attributes.value: sc-229
attributes.tag: treatment
attributes.value: EtOH_0.02pct
attributes.tag: treatment description
attributes.value: 1 h with 0.02% Ethanol (Myers)
attributes.tag: protocol
attributes.value: v042211.1
attributes.tag: protocol description
attributes.value: Faster ChIP protocol & AMpure XP size selection for ChIP-seq (Myers)
attributes.tag: controlid
attributes.value: SL6021
attributes.tag: labexpid
attributes.value: SL7279,SL6593
attributes.tag: replicate
attributes.value: 1,2
attributes.tag: softwareversion
attributes.value: MACS