title: Bob
organism: Homo sapiens
description: Protocols: The healthy control hiPSC lines Yemz1, Lise1 and Kolf2 were acquired through the Human Induced Pluripotent Stem Cells Initiative Consortium (HipSci www.hipsci.org). The line Bob(CA1ATD) was generated in the Lab for regenerative medicine, Wellcome Trust MRC Cambridge Stem Cell Institute, as described in Yusa et al, Nature. 2011 Oct 12;478(7369):391-4. doi: 10.1038/nature10424. All iPSC lines originate from fibroblasts. Derivation and growth of iPSC-derived human intestinal epithelial organoids followed the protocol described by Forbester et al., Methods Mol Biol. 2016 Aug 31. [Epub ahead of print] Derivation of Intestinal Organoids from Human Induced Pluripotent Stem Cells for Use as an Infection System; also chacracterised in Forbester et al, Infect Immun. 2015 Jul;83(7):2926-34. doi: 10.1128/IAI.00161-15. Samples were harvested after at least 12 passages in culture. RNA was extracted using a commercially available kit (Qiagen) according to manufacturer's instructions. Libraries for RNA sequencing were prepared with Illumina TruSeq protocol (Illumina, Cambridge, UK).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-10-22T17:03:18Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-10-17T16:13:20Z
attributes.tag: External Id
attributes.value: SAMEA4981173
attributes.tag: INSDC center name
attributes.value: University of Cambridge Department of Paediatrics
attributes.tag: INSDC first public
attributes.value: 2018-10-22T17:03:18Z
attributes.tag: INSDC last update
attributes.value: 2018-10-17T16:13:20Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7306:Bob
attributes.tag: age
attributes.value: >18
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: Bob
attributes.tag: cell type
attributes.value: epithelial cell of small intestine
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: normal
attributes.tag: growth condition
attributes.value: intestinal epithelial organoid culture
attributes.tag: progenitor cell type
attributes.value: induced pluripotent stem cell
attributes.tag: sample name
attributes.value: E-MTAB-7306:Bob
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: male