title: WW Selection II, Round 3 Library
organism: Homo sapiens
description: A collection of the Human Yap65 WW variants were obtained using degenerate chemical synthesis. Variants contain a 102 nucleotide variable region. Following a short PCR amplification to yield double-stranded DNA molecules, variants were cloned into the T7 phage packaging extract for phage display. Phage displaying hYap65 variants were submitted to three rounds of selection for binding to the cognate peptide ligand (GTPPPPYTVG) on beads. Illumina sequencing libraries were prepared from phage of the unselected (Input) library, as well as after the first (Round 1), second (Round 2) and third (Round 3) rounds of selection. Staggered, overlapping 76bp paired-end reads were acquired using the Illumina GAIIx platform to sequence the 102 nucleotide variable regions in each library.