title: Onion normalized cDNA from DH5225
organism: Allium cepa
description: Total RNA extraction was carried out using a modified Trizol method (Invitrogen, USA) from each of four tissues (roots, leaves, umbels, and bulbs) collected from the onion double haploid line DH 5225. Isolated RNA was quantified and pooled in equal amounts from each of the tissue-type for cDNA synthesis using a modified SMART (Switching Mechanism At 5' end of RNA Transcript) cDNA synthesis method.  Size fractionation of re-amplified cDNA was carried out on an agarose gel to isolate 0.5 kb and longer cDNA fragments by electro-elution.  Samples were collected by the Michael Havey group at the University of Wisconsin.  RNA extraction, and cDNA synthesis and normalization were carried out by Bio S&amp;T, Canada.