title: Sample 10
organism: Mus musculus
description: Protocols: Mice carrying a conditional H2-Ab1 allele (H2-Ab1c) (Hashimoto, K., Joshi, S. K. & Koni, P. A. A conditional null allele of the major histocompatibility IA-beta chain gene. Genesis 32, 152-153 (2002)) were crossed to mice expressing Cre in DCs under the Itgax (CD11c-Cre driver; Itgaxcre) ( Caton, M. L., Smith-Raska, M. R. & Reizis, B. Notch-RBP-J signaling controls the homeostasis of CD8- dendritic cells in the spleen. J. Exp. Med. 204, 1653-1664 (2007)). This Cre transgenic mouse strain will delete the gene encoding the H2-Ab chain in all dendritic cells, as well as in a proportion of other hematopoietic cells. MHC II expression is developmentally regulated and begins in the bone marrow. Cells that have not yet expressed MHC II and those that have started expressing were compared from both wild-type and ItgaxcreH2-Ab1c MHC II conditional mice. Bone marrow pre-B cells (Hardy's Fraction D) and splenic follicular B cells were purified by flow cytometry according to MHC II protein expression from wild-type and ItgaxcreH2-Ab1c MHC II conditional mice and used for the isolation of RNA. RNA was then extracted using the RNeasy Mini QIAcube or Micro kits (Qiagen) Illumina, RNA with PolyA selection (performed by GeneWiz)
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-08-07T17:02:06Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-05-15T10:54:52Z
attributes.tag: External Id
attributes.value: SAMEA4663624
attributes.tag: INSDC center name
attributes.value: Retroviral Immunology The Francis Crick Institute
attributes.tag: INSDC first public
attributes.value: 2018-08-07T17:02:06Z
attributes.tag: INSDC last update
attributes.value: 2018-05-15T10:54:52Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6765:Sample 10
attributes.tag: age
attributes.value: 6 to 12
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: Pre-B cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: ItgaxcreH2-Ab1c
attributes.tag: organism part
attributes.value: bone marrow
attributes.tag: phenotype
attributes.value: MHC II-negative
attributes.tag: sample name
attributes.value: E-MTAB-6765:Sample 10
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: mixed
attributes.tag: strain
attributes.value: C57BL/6