title: Sample 41
organism: Homo sapiens
description: Protocols: Circulating tumour cells were collected from patient blood using RosetteSep (Stemcell Technologies) and injected into the flank of non-obese diabetic severe combined immunodeficient interleukin-2 receptor γ-deficient mice. Tumours were excised from terminally sacrificed mice, and tumour fragments were immediately transferred to 10 volumes of RNAlater (Qiagen) and stored overnight at 4°C. Tumour fragments were then stored at -80°C until further processing. Three independent tumours from each model were homogenized in RLT buffer (Qiagen) using the TissueLyser LT (Qiagen) and FastPrep Lysing Matrix A tubes (MP Biomedicals). RNA was extracted with RNeasy mini kits (Qiagen) and quantified using Qubit (Thermo Fisher). RNA-seq data were aligned to Human GRCh38 and Mouse GRCm38 assembly using Mapsplice (version 2.1.6). Mouse reads were subsequently filtered from the data.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-04-27T04:05:48Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-01-13T10:57:01Z
attributes.tag: External Id
attributes.value: SAMEA6469304
attributes.tag: INSDC center name
attributes.value: CRUK-MI
attributes.tag: INSDC first public
attributes.value: 2020-04-27T04:05:48Z
attributes.tag: INSDC last update
attributes.value: 2020-01-13T10:57:01Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8465_2:Sample 41
attributes.tag: age
attributes.value: 66
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: clinical information
attributes.value: progression
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: small cell lung carcinoma
attributes.tag: growth condition
attributes.value: human derived xenograft implanted in immunodeficient mice
attributes.tag: individual
attributes.value: CDX20P
attributes.tag: organism part
attributes.value: lung
attributes.tag: replicate
attributes.value: 2
attributes.tag: response to treatment
attributes.value: Stable Response
attributes.tag: sample name
attributes.value: E-MTAB-8465_2:Sample 41
attributes.tag: sampling site
attributes.value: xenograft
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: male
attributes.tag: xenograft
attributes.value: patient derived xenograft