title: Sample 34
organism: Mus musculus
description: Protocols: Brains of wt (C57Bl6/J) and TNFtg mice were removed and cut into hemispheres. Cerebellum, hippocampus, thalamus, striatum, and cortex from both hemispheres were immediately dissected on ice and stored at -80°C. Dissected right hemispheric regions were mechanically homogenized in QIAzol reagent (Qiagen, Hilden, Germany) and RNA was isolated using RNeasy Plus Mini Kit (Qiagen). The attained concentration of RNA was determined using the NanoDrop spectrophotometric system (Peqlab, Erlangen, Germany). Poly(A) selection of RNA was performed as previously described (Gosselin et al., 2014). Briefly, RNA samples were incubated with Oligo d(T) Magnetic Beads (NEB, Ipswich, MA) and poly(A) enriched RNA was collected. To fragment poly(A) RNA, samples were incubated at 94 °C for 9 min on beads. First-strand synthesis and second-strand synthesis as well as blunting, A-tailing, and adapter ligation was done using barcoded adapters (NextFlex, Bioo Scientific) as previously described(Gosselin et al., 2014). Libraries were PCR-amplified for 12-15 cycles and size selected for fragments (200-350 bp) by gel extraction (10% TBE gels, Life Technologies EC62752BOX).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-10-22T17:02:14Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-10-12T17:23:30Z
attributes.tag: External Id
attributes.value: SAMEA4973340
attributes.tag: INSDC center name
attributes.value: University of California San Diego Department of Cellular and Molecular Medicine
attributes.tag: INSDC first public
attributes.value: 2018-10-22T17:02:14Z
attributes.tag: INSDC last update
attributes.value: 2018-10-12T17:23:30Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7279:Sample 34
attributes.tag: age
attributes.value: 12 to 14
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: disease
attributes.value: normal
attributes.tag: individual
attributes.value: GI92
attributes.tag: organism part
attributes.value: dorsal plus ventral thalamus
attributes.tag: phenotype
attributes.value: wild type
attributes.tag: sample name
attributes.value: E-MTAB-7279:Sample 34
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: C57Bl6/J