title: Surface water
organism: marine metagenome
description: Surface water at station St.1
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2012-07-24T17:00:31Z
attributes.tag: ENA-KEYWORD
attributes.value: GSC, MIxS, MIMARKS
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-09T09:57:04Z
attributes.tag: External Id
attributes.value: SAMEA1469945
attributes.tag: Geographic location (country:region,area)
attributes.value: Kongsfjorden, Spitsbergen
attributes.tag: INSDC center name
attributes.value: PRIC
attributes.tag: INSDC first public
attributes.value: 2012-07-24T17:00:31Z
attributes.tag: INSDC last update
attributes.value: 2018-03-09T09:57:04Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: St.1
attributes.tag: amount or size of sample collected
attributes.value: 12
attributes.tag: broker name
attributes.value: EMG
attributes.tag: depth
attributes.value: 0.1
attributes.tag: elevation
attributes.value: 0
attributes.tag: enviornmental package
attributes.value: water
attributes.tag: environment (biome)
attributes.value: marginal sea biome
attributes.tag: environment (featurel)
attributes.value: marine habitat
attributes.tag: environment (material)
attributes.value: sea water
attributes.tag: geographic location (latitude)
attributes.value: 78.98N
attributes.tag: geographic location (longitude)
attributes.value: 11.65E
attributes.tag: investigation type
attributes.value: MIxS
attributes.tag: library reads sequenced
attributes.value: 7651
attributes.tag: multiplex identifiers
attributes.value: AGACGTGATC
attributes.tag: pcr conditions
attributes.value: initial denaturation:95degC_2min;denaturation:95degC_30sec;annealing:55degC_30sec;elongation:72degC_30sec;final elongation:72degC_5min;total
attributes.tag: pcr primers
attributes.value: FWD:8F 5'-AGAGTTTGATCCTGGCTCAG-3';REV:533R 5'-TTACCGCGGCTGCTGGCAC-3'
attributes.tag: project name
attributes.value: Diversity of marine planktonic bacteria in polar regions
attributes.tag: sample collection device or method
attributes.value: 20 liter Niskin bottle
attributes.tag: sample material processing
attributes.value: 0.2 micrometer cellulose acetate filtration
attributes.tag: sample name
attributes.value: St.1
attributes.tag: scientific_name
attributes.value: marine metagenome
attributes.tag: sequencing method
attributes.value: GS FLX + System 454 pyrosequencing
attributes.tag: sequencing method
attributes.value: Pyrosequencing
attributes.tag: target gene
attributes.value: 16S rRNA