title: Membranous bracts at the base of a cereal seed (PO:0006367).
organism: Hordeum vulgare subsp. vulgare
description: Barley (Hordeum vulgare L.) plants of cv. Barke were grown under standard greenhouse conditions at 18 degree Celsius with 16 h of light. Developmental stages for barley grains were determined after Weschke et al. (2000). Flag leaves, glumes and grain tissues were collected based on grain developmental stages. For flag leaves and glumes, samples were collected in two day interval starting from 4 days before anthesis until 24 days after flowering (DAF). Grains from 0, 4, 8, 10, 12, 14 DAF were manually dissected into the maternal and the filial parts and whole caryopses were sampled at 16, 20 and 24 DAF. Total RNA was isolated separately from each tissue and stage using Purescript RNA isolation kit (Biozym, Hamburg, Germany). To prepare RNA samples for transcriptome sequencing of flag leaves, glumes and grains, equal amounts of RNA from all stages were united for each tissue to achieve 27 microgramme of RNA for each organ. Pyrosequencing of the three libraries was done by GATC Biotech (www.gatc-biotech.com).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2012-09-04T09:38:46Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T15:40:22Z
attributes.tag: External Id
attributes.value: SAMEA1500965
attributes.tag: INSDC center name
attributes.value: IPK-Gatersleben
attributes.tag: INSDC first public
attributes.value: 2012-09-04T09:38:46Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T15:40:22Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: glumes
attributes.tag: common name
attributes.value: domesticated barley
attributes.tag: sample name
attributes.value: glumes
attributes.tag: scientific_name
attributes.value: Hordeum vulgare subsp. vulgare
attributes.tag: sub_species
attributes.value: vulgare