title: ZT8-Col0-3_S3
organism: Arabidopsis thaliana
description: Protocols: Whole seedlings were harvested and frozen in liquid nitrogen. Wild type Col-0 and frs7-1;frs12-1 mutants were sown in MS-agar plates and stratified for 3 days at 4°C. After stratification, seedlings were grown in SD (8 h light: 16 hours dark) conditions for 10 days. SD (8 h light: 16 hours dark) conditions for 10 days. Seedlings were harvested at ZT8 (2 pm) and ZT16 (10 pm). RNA was isolated using the RNeasy Plant Mini Kit (Qiagen) and DNase I treated (Promega). The library of cDNA template molecules of known strand origin was obtained using the reagents provided in the Illumina TruSeq Stranded mRNA Sample Preparation Kits.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-04-27T17:07:02Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-11-15T10:41:34Z
attributes.tag: External Id
attributes.value: SAMEA5089879
attributes.tag: INSDC center name
attributes.value: Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, Belgium VIB Center for Plant Systems Biology,VIB, Gent, Belgium
attributes.tag: INSDC first public
attributes.value: 2020-04-27T17:07:02Z
attributes.tag: INSDC last update
attributes.value: 2018-11-15T10:41:34Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7397:ZT8-Col0-3_S3
attributes.tag: age
attributes.value: 10
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: thale cress
attributes.tag: developmental stage
attributes.value: seedling development stage
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: whole organism
attributes.tag: sample name
attributes.value: E-MTAB-7397:ZT8-Col0-3_S3
attributes.tag: scientific_name
attributes.value: Arabidopsis thaliana
attributes.tag: ecotype
attributes.value: Col-0