title: Jouvenceaux
organism: soil metagenome
description: DNA was extracted from outcropping fibrous tremolite with calcite collected from masses along road sides. Four samples of fibrous substrate were collected in this site and the DNA extracted (three replicate extraction per sample) was pooled. Total DNA extraction was performed by the Fast DNA Spin Kit for Soil (Q-BIOgene, Heidelberg; Germany), using the protocol modified by Luis et al. 2005.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2012-08-31T17:01:42Z
attributes.tag: ENA-KEYWORD
attributes.value: GSC, MIxS, MIMARKS
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-09T09:58:02Z
attributes.tag: External Id
attributes.value: SAMEA1487339
attributes.tag: Geographic location (country:region,area)
attributes.value: Italy
attributes.tag: INSDC center name
attributes.value: unito
attributes.tag: INSDC first public
attributes.value: 2012-08-31T17:01:42Z
attributes.tag: INSDC last update
attributes.value: 2018-03-09T09:58:02Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: Jouvenceaux
attributes.tag: adapters
attributes.value: for ITS1 region: FW 5'- GCCTCCCTCGCGCCATCAGAACTTGGTCATTTAGAGGAAGTAA-3' and REV 5'- GCCTTGCCAGCCCGCTCAGGCTGCGTTCTTCATCGATGC-3'; for ITS2 region: FW 5'- GCCTCCCTCGCGCCATCAGTCGCATCGATGAAGAACGCAGC-3' and REV 5'- GCCTTGCCAGCCCGCTCAGTCCTCCGCTTATTGATATGC-3'
attributes.tag: broker name
attributes.value: EMG
attributes.tag: collection date
attributes.value: 2005-11-01
attributes.tag: current land use
attributes.value: Outcrop of fibrous tremolite with calcite, in an unused field, along road sides.
attributes.tag: depth
attributes.value: 0
attributes.tag: dry mass
attributes.value: 6 g
attributes.tag: enviornmental package
attributes.value: soil
attributes.tag: environment (biome)
attributes.value: Temperate broadleaf and mixed forest biome
attributes.tag: environment (featurel)
attributes.value: terrestrial habitat
attributes.tag: environment (material)
attributes.value: piece of rock
attributes.tag: geographic location (latitude)
attributes.value: 45.02476944444
attributes.tag: geographic location (longitude)
attributes.value: 6.84587222222
attributes.tag: investigation type
attributes.value: MIxS
attributes.tag: ph
attributes.value: 7
attributes.tag: ph method
attributes.value: DeGrood SH, Claassen VP, Scow KM (2005) Microbial community composition on native and drastically disturbed serpentine soils. Soil Biol Biochem 37: 1427-1435. -Benton Jones Jr. J. (2001) Laboratory guide for conducting soil tests and plant analysis. CRC Press, Boca Raton. 363 p.
attributes.tag: project name
attributes.value: Fungi inhabiting Western Alps serpentine substrates
attributes.tag: sample name
attributes.value: Jouvenceaux
attributes.tag: scientific_name
attributes.value: soil metagenome
attributes.tag: sequencing method
attributes.value: Pyrosequencing
attributes.tag: target gene
attributes.value: ITS1 and ITS2