title: Onion normalized cDNA from OH-1
organism: Allium cepa
description: Total RNA extraction was carried out using a modified Trizol method (Invitrogen, USA) from each of four tissues (roots, leaves, umbels, and bulbs) collected from the yellow onion inbred line OH-1. Isolated RNA was quantified and pooled in equal amounts from each of the tissue-type for cDNA synthesis using a modified SMART (Switching Mechanism At 5' end of RNA Transcript) cDNA synthesis method.  Size fractionation of re-amplified cDNA was carried out on an agarose gel to isolate 0.5 kb and longer cDNA fragments by electro-elution.  Samples were collected by the Michael Havey group at the University of Wisconsin.  RNA extraction, and cDNA synthesis and normalization were carried out by Bio S&T, Canada.