title: Sample 1
organism: Sungouiella intermedia
description: Protocols: Samples were collected to follow growth, carbon source consumption and product formation. The yeast strain was cultivated in a DasGip system, using 4 reactors per condition. The different conditions were prepared with standard approach to grow yeast in reactors. The following conditions were used: Carbon source. (g/L) Dissolved oxygen (percentage): Glucose (30) <10%; Glucose (10) + Xylose (20) <10%; Glucose (20) 30-40%; Glucose (200) 30-40%; Xylose (20) 30-40%; Xylose (200) 30-40%; Lactose (20) 30-40%; Galactose (20) 30-40%; Cellobiose (20) 30-40% TRIzol (also known as TRI REAGENT) for the isolation of total RNA. TRIzol is a mixture of guanidine thioacyanate and phenol, which effectively dissolves DNA, RNA and protein on homogenization or lysis of tissue sample. After adding chloroform and centrifuging, the mixture separates into 3 phases with the upper clear aqueous phase containing the RNA. The next steps include transfer of the clear upper phase to 1 volume of absolute ethanol, and then transfer the mixture to an RNeasy column, following the kit instructions. TrueSeq
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-06-29T17:01:50Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-04-17T13:58:21Z
attributes.tag: External Id
attributes.value: SAMEA4589529
attributes.tag: INSDC center name
attributes.value: National Bioinformatics Infrastructure Sweden, Science for Life Laboratory, Uppsala University
attributes.tag: INSDC first public
attributes.value: 2018-06-29T17:01:50Z
attributes.tag: INSDC last update
attributes.value: 2018-04-17T13:58:21Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6670:Sample 1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: growth condition
attributes.value: cellobiose 20 grams per liter; 30 to 40 percent dissolved oxygen
attributes.tag: sample name
attributes.value: E-MTAB-6670:Sample 1
attributes.tag: scientific_name
attributes.value: [Candida] intermedia
attributes.tag: strain
attributes.value: CBS 141442