title: polyA+_testis_7060321
organism: Homo sapiens
description: Protocols: RNA from tissues was isolated by a modified guanidium thiocynate methods followed by polyA+ RNA selection with two rounds of oligo(dT)-cellulose columns. 5' and 3' RACE reactions were performed in 12.5 ul volume independently with brain and testis polyA+ RNA with the SMART RACE amplification kit (Clontech) using manufacturer's instructions. 7.5ul of each of the 400 lncRNA targets were pooled respecting tissues and directionality of the RACE reaction (5' and 3' RACE) and purified with the QIAquick kit (Quiagene) as described for RT-PCR-seq experiments (Howald et al. 2012). A set of 25 control reactions targeting full-length annotated transcripts were RACEd in parallel as positive controls. 1.5ul of these controls were pooled with the other reactions prior to library preparation.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2012-09-19T17:01:34Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T15:59:37Z
attributes.tag: External Id
attributes.value: SAMEA1523778
attributes.tag: INSDC center name
attributes.value: Wellcome Trust Sanger Institute
attributes.tag: INSDC first public
attributes.value: 2012-09-19T17:01:34Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T15:59:37Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-1222:polyA+_testis_7060321
attributes.tag: bio source type
attributes.value: frozen sample
attributes.tag: biosourceprovider
attributes.value: Clontech
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: human
attributes.tag: organism part
attributes.value: testis
attributes.tag: sample name
attributes.value: E-MTAB-1222:polyA+_testis_7060321
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: male