title: Patient66diag_NSG_I_18255
organism: Homo sapiens
description: Protocols: humanCD45 enriched xenograft samples from NSG or NSG-S xentransplanted mice with JMML CD34+ cells. For secondary xenografts, bone marrow cells from primary engrafted mice were injected in another mouse to test the self-renewal capacities of JMML cells. The bone marrow cells of the primary mice were not sorted before the injection into the secondary recipient because the percentage of human CD45 was high. Qiagen Mini or Midi Kit (Qiagen Gmbh) Exome capture was carried out using the SureSelect Human All Exon V5 or SureSelect Clinical Research (Agilent Technologies, Santa Clara, CA, USA) according to manufacturer's instruction and protocols.
attributes.tag: ENA first public
attributes.value: 2018-03-15
attributes.tag: ENA last update
attributes.value: 2018-02-06
attributes.tag: External Id
attributes.value: SAMEA104569305
attributes.tag: INSDC center alias
attributes.value: Assistance Publique des Hopitaux de Paris (AP-HP), Hopital Robert Debre, Departement de Genetique, Paris, France
attributes.tag: INSDC center name
attributes.value: Assistance Publique des Hopitaux de Paris (AP-HP), Hopital Robert Debre, Departement de Genetique, Paris, France
attributes.tag: INSDC first public
attributes.value: 2018-03-15T17:01:43Z
attributes.tag: INSDC last update
attributes.value: 2018-02-06T16:19:59Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6467:Patient66diag_NSG_I_18255
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: mononuclear cell
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: juvenile myelomonocytic leukemia
attributes.tag: disease staging
attributes.value: diagnosis
attributes.tag: individual
attributes.value: patient66
attributes.tag: phenotype
attributes.value: human CD45 positive
attributes.tag: sample name
attributes.value: E-MTAB-6467:Patient66diag_NSG_I_18255
attributes.tag: sampling time point
attributes.value: primary xenograft
attributes.tag: xenograft mouse strain
attributes.value: NSG