title: 3417-MAM-1
organism: Mus musculus
description: Protocols: Ins2Apple/+ pancreatic islets were collected from mice at 8-9 weeks of age. Mice were segregated by sex, and the islets from 2-3 mice of the same sex and genotype were pooled for each sample. Islets were dispersed using Accumax and FACS purified using Apple fluorescence and DAPI viability dye, to collect only live pancreatic beta cells. Abcc8 KO and wild type mice containing Ins2Apple/+ allele were maintained in a C57BL/6J background. All mice used for experiments were fed either a standard chow diet or a 60% high fat diet for 5 week and were maintained on a 12-hour light/dark cycle. Total RNA was extracted from FACS-purified pancreatic beta cells using the Maxwell simplyRNA kit (Promega). RNA was amplified using the SMART-Seq v4 Ultra Low Input RNA Kit (Takara), and cDNA libraries were constructed using the Low Input Library Prep Kit (Takara).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-04-27T04:05:48Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-01-09T13:26:16Z
attributes.tag: External Id
attributes.value: SAMEA6461401
attributes.tag: INSDC center name
attributes.value: VANDERBILT UNIVERSITY
attributes.tag: INSDC first public
attributes.value: 2020-04-27T04:05:48Z
attributes.tag: INSDC last update
attributes.value: 2020-01-09T13:26:16Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8643:3417-MAM-1
attributes.tag: age
attributes.value: 60
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: beta cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: diet
attributes.value: normal
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: islet of Langerhans
attributes.tag: sample name
attributes.value: E-MTAB-8643:3417-MAM-1
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: C57BL/6J