title: ZmNLS-GSyellow
organism: Zea mays
description: Protocols: Leaf samples were always taken during steady state growth of the leaf (2d after the appearance of leaf4). Samples of four centimeter were pooled, encompassing the division zone, transition zone and the expansion zone. For each maize sample, 1.2 g leaf tissue (leaf 4 cm1-4) was fixed in Buffer A (Morohashi et al., 2012) for 15 min under vacuum conditions. For quenching, glycine was added to a final concentration of 0.1 M and incubated for 10 min. Fixed leaf tissue was washed with distilled water and frozen in liquid N2. ChIP-seq on PPD2-GSyellow and ZmAN3-GSyellow was performed using 5 L ab290 anti-GFP antibody (Abcam), as described in Morohashi et al., 2012. ChIP-seq libraries were prepared by GATC Biotech
attributes.tag: ENA first public
attributes.value: 2018-08-07
attributes.tag: ENA last update
attributes.value: 2018-02-21
attributes.tag: External Id
attributes.value: SAMEA104605991
attributes.tag: INSDC center alias
attributes.value: Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, Belgium VIB Center for Plant Systems Biology,VIB, Gent, Belgium
attributes.tag: INSDC center name
attributes.value: Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, Belgium VIB Center for Plant Systems Biology,VIB, Gent, Belgium
attributes.tag: INSDC first public
attributes.value: 2018-08-07T17:03:06Z
attributes.tag: INSDC last update
attributes.value: 2018-02-21T16:05:02Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6527:ZmNLS-GSyellow
attributes.tag: age
attributes.value: 2 days after emergence of leaf 4
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cultivar
attributes.value: B104
attributes.tag: genotype
attributes.value: ZmNLS-GSyellow
attributes.tag: organism part
attributes.value: leaf
attributes.tag: sample name
attributes.value: E-MTAB-6527:ZmNLS-GSyellow
attributes.tag: sampling site
attributes.value: basal 4 cm of growing leaf 4