organism: Homo sapiens
attributes.tag: library_ID
attributes.value: 297
attributes.tag: treatment
attributes.value: TBE 32 A 2 Cas9 3G RNP PD1_R1
attributes.tag: ref_seq
attributes.value: ACCCTCCCTTCAACCTGACCTGGGACAGTTTCCCTTCCGCTCACCTCCGCCTGAGCAGTGGAGAAGGCGGCACTCTGGTGGGGCTGCTCCAGGCATGCAGATCCCACAGGCGCCCTGGCCAGTCGTCTGGGCGGTGCTACAACTGGGCTGGCGGCCAGGATGGTTCTTAGGTAGGTGGGGTCGGCGGTCAGGTGTCCCAGAGCCAGGGGTCTGGAGGGACCTTCCACCCTCAGTCCCTGGCAGGTCGGGGGGTGCTGAGGCGGGCCTGGCCCTGGCAGCCCAGGGGTCCCGGAGCGAGGGGTCTGGAGGGACCTTTCACTCTCAGTCCCTGGCAGGTCGGGGGGTGCTGTGGCAGGCCCAGCCTTGGCCCCCAGCTCTGCCCCTTACCCTGAGCTGTGTGGCTT
attributes.tag: guide
attributes.value: CACCTAcCTAAGAACCATCC
attributes.tag: % C
attributes.value: 99.04
attributes.tag: % T
attributes.value: 0.58
attributes.tag: % G
attributes.value: 0.17
attributes.tag: % A
attributes.value: 0.21
attributes.tag: % WT length
attributes.value: 23.78
attributes.tag: % Indel
attributes.value: 76.22
attributes.tag: % Out-of-Frame Indel
attributes.value: 53.98
attributes.tag: % In-Frame Indel
attributes.value: 22.24
attributes.tag: Reads
attributes.value: 56464
attributes.tag: Donor Number
attributes.value: 020537
attributes.tag: Method of Isolation
attributes.value: NA
attributes.tag: Biomaterial Provider
attributes.value: NA
attributes.tag: sex
attributes.value: NA
attributes.tag: tissue
attributes.value: NA
attributes.tag: Notes
attributes.value: NA
attributes.tag: Read
attributes.value: R1
attributes.tag: isolate
attributes.value: Memorial blood donor
attributes.tag: age
attributes.value: Unknown
attributes.tag: biomaterial provider
attributes.value: Branden Moriarity
attributes.tag: title
attributes.value: Highly efficient multiplex human T cell engineering without double-strand breaks using Cas9 base editors
attributes.tag: library_strategy
attributes.value: Amplicon NGS
attributes.tag: library_source
attributes.value: PCR of gDNA
attributes.tag: library_selection
attributes.value: None
attributes.tag: library_layout
attributes.value: Individual amplicons
attributes.tag: platform
attributes.value: Illumina MiSeq
attributes.tag: instrument_model
attributes.value: Unknown
attributes.tag: design_description
attributes.value: 2 x 250 paired end reads
attributes.tag: filetype
attributes.value: .fastq.gz
attributes.tag: filename
attributes.value: TBE_32__A__2__Cas9_3G_RNP__PD1_S43_R1_001.fastq.gz
attributes.tag: BioSampleModel
attributes.value: Human