title: L351
organism: Homo sapiens
description: Protocols: Cell centrifugation Three clones with ETO2-GLIS2 and CTRL iPSC were induced to differentiate into hematological lineage using 2D co-culture system on matrix adapted from Chou S et al. Briefly 3-4 colonies of each iPSC were picked and put into Geltrex (Thermofisher) coated wells in Essential 8 medium. For the first 2 days, the cells are grown in StemPro34 medium supplemented with 1X penicillin/streptomycin (PS, GIBCO), glutamine (2 mM), ascorbic acid (50 μg/ml, Sigma) monothioglycerol (MTG, 15 mM, Sigma), BMP4 (5 ng/mL) and VEGF (50 ng/ml) and CHIR (930 ng/mL). The medium was replenished and supplemented until day 18 with a sequential cytokine cocktail, as shown in Table 2. Hematopoietic differentiation of iPSC was then assessed at day 13, 15 and 18 using flow cytometry analyses for hematopoietic progenitors (CD34, CD43) and megakaryocytic markers (CD41, CD42 and CD61). All data were analyzed with FACSCantoII or Fortessa with FACS Diva software (BD Biosciences). Qiagen RNeasy TruSeq Stranded mRNA kit (Illumina) following manufacturers' instructions
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-04-27T08:05:51Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-05-15T15:13:51Z
attributes.tag: External Id
attributes.value: SAMEA5609493
attributes.tag: INSDC center name
attributes.value: INSERM, France Gustave Roussy institute, France Universite Paris Saclay, France
attributes.tag: INSDC first public
attributes.value: 2020-04-27T08:05:51Z
attributes.tag: INSDC last update
attributes.value: 2019-05-15T15:13:51Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7967:L351
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: iPSC derived
attributes.tag: cell type
attributes.value: hematopoietic cell
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: pediatric leukemia
attributes.tag: genotype
attributes.value: ETO2-GLIS2
attributes.tag: organism part
attributes.value: hematopoietic system
attributes.tag: phenotype
attributes.value: CD41+CD42+
attributes.tag: progenitor cell type
attributes.value: induced pluripotent stem cell
attributes.tag: sample name
attributes.value: E-MTAB-7967:L351
attributes.tag: sampling time point
attributes.value: 18
attributes.tag: scientific_name
attributes.value: Homo sapiens