title: Model organism or animal sample from Misgurnus anguillicaudatus
organism: Misgurnus anguillicaudatus
description: The fish analyzed in the RNA-seq experiments were grown in normal conditions. They were originally caught in the Sichuan province of China. Individual fish were taken to the laboratory and housed in one aquarium, which was maintained at a water temperature of 24 to 26 Celsius, pH from 6.5 to 7.5 and within a natural photoperiod. For sampling, six fish (3 females, 3 males) were anesthetized using MS-222 (100 mg/L, Sigma Chemical Company, St Louis, MO) and the following tissues immediately harvested: liver, muscle, kidney, intestine, gonads and brain. Tissues from the 6 fish were mixed, flash frozen in liquid nitrogen and placed in a -80 Celsius freezer prior to processing for total RNA isolation.
attributes.tag: isolate
attributes.value: Sichuan province of China
attributes.tag: dev_stage
attributes.value: Adult fish
attributes.tag: sex
attributes.value: pooled male and female
attributes.tag: tissue
attributes.value: liver, muscle, kidney, intestine, gonads and brain
attributes.tag: isol_growth_condt
attributes.value: missing
attributes.tag: Project_name
attributes.value: Misgurnus anguillicaudatus Transcriptome
attributes.tag: Lat_lon
attributes.value: 30.083056 N 103.784722 E
attributes.tag: geo_loc_name
attributes.value: China: Sichuan
attributes.tag: Collected_by
attributes.value: Qing Wu
attributes.tag: Collection_date
attributes.value: 2018-05
attributes.tag: Environment (biome)
attributes.value: ENVO:00000078
attributes.tag: Environment (feature)
attributes.value: ENVO:00000077
attributes.tag: Environment (material)
attributes.value: ENVO:00000294
attributes.tag: Sample type
attributes.value: Liver, muscle, kidney, intestine, gonads and brain
attributes.tag: Developmental stage
attributes.value: Adult
attributes.tag: Sequencing method
attributes.value: PACBIO SMRT Sequel
attributes.tag: Assembly method
attributes.value: PACBIO SMRT
attributes.tag: Assembly name
attributes.value: Misgurnus anguillicaudatus Transcriptome
attributes.tag: Data accessibility
attributes.value: BioProject: PRJNA596116
attributes.tag: BioSampleModel
attributes.value: MIGS.eu