title: Biofilm Sample2 (Ship#2)
organism: Tenacibaculum maritimum
description: Biofilm samples were harvested in August, 2010 from the hulls of the USS Bainbridge (Ship#2) at Norfolk Naval Station in Norfolk, VA, USA. The ship hull was sampled after a seven month deployment (January – July, 2010) that started and concluded in Norfolk, VA. Biofilms with a Fouling Rating 20 (i.e. “advanced slime” - Naval Ships’ Technical Manual Chapter 081, 2006) were scraped from the air-water interface (starboard, mid-ship) and immediately snap frozen in sterile 50 mL conical tubes using an EtOH-dry ice bath (Supplementary Figure S1). Surface water samples were also collected at a distance of 0.5 to 5.0 m from the hulls of the same vessels and all planktonic material was harvested on 0.22 *m UltraClean Water DNA Filters (MO BIO Laboratories, Inc. Carlsbad, CA, USA) and snap frozen.