title: cardio-2
organism: Homo sapiens
description: Protocols: We used the iPSC line NA19101 (https://www.coriell.org/0/Sections/Search/Sample_Detail.aspx?Ref=GM19101&product=CC) to generate cardiomyocytes. Cardiomyocyte differentiations were carried out similar to the protocol of Burridge et al, 2013 with slight modifications. Briefly, we initiated mesoderm differentiation of the iPSCs with the GSK-3 inhibitor CHIR-99021 trihydrochloride for 24 hours (Tocris #4953). Two days later, cardiomyocyte differentiation was initiated with 2μM Wnt-C59 (Tocris #5148) for 48 hours. Cells exhibited spontaneous beating by day 8-10 and were subsequently selected with lactate on days 15-20 at which time they were harvested and analyzed with flow cytometry for cardiac troponin T. Total RNA was extracted from flash-frozen pellets of 1 million cells using TRI Reagent (Sigma #T9424) and a homogenizer followed by RNA isolation and clean-up using the Direct-zol RNA Kit (Zymo Research #11-331). RNA-seq libraries were generated with the Illumina TruSeq V2 kit (Illumina, RS-122-2001) and 1μg of RNA, following manufacturer's instructions.
attributes.tag: ENA first public
attributes.value: 2018-03-15
attributes.tag: ENA last update
attributes.value: 2018-03-05
attributes.tag: External Id
attributes.value: SAMEA104666170
attributes.tag: INSDC center alias
attributes.value: University of Chicago
attributes.tag: INSDC center name
attributes.value: University of Chicago
attributes.tag: INSDC first public
attributes.value: 2018-03-15T17:01:45Z
attributes.tag: INSDC last update
attributes.value: 2018-03-05T11:27:13Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6013:cardio-2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: cardiac muscle cell
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: normal
attributes.tag: ethnic group
attributes.value: Yoruba
attributes.tag: individual
attributes.value: NA19101
attributes.tag: organism part
attributes.value: blood
attributes.tag: progenitor cell type
attributes.value: induced pluripotent stem cell
attributes.tag: sample name
attributes.value: E-MTAB-6013:cardio-2
attributes.tag: sex
attributes.value: male