title: do928
organism: Mus musculus
description: Protocols: All mice used in this study were housed and handled in accordance with the Animals (Scientific Procedures) Act 1986. Mice were housed and cared for in the Cambridge Research Institute Biological Resource Unit with a twelve hour light/dark cycle, and were provided with chow food from Lab Diet plus water ad libitum. Mice were sacrificed by cervical dislocation at 4-6 months of age, between the hours of 9:30am and 11:30am. The liver was then cut and the mouse perfused with PBS by injection into the heart. Subsequently, the liver was removed and the gall bladder and hepatic vein cut away. A sample of approximately 50mg-100mg was taken from a single liver lobe and frozen on liquid nitrogen for storage at -80C until use. For each liver sample, total RNA was extracted using the RNeasy Mini kit (Qiagen 74104) as per manufacturer's instructions. Before further processing, each total RNA sample was treated to digest contaminating DNA. From each sample, polyadenylated mRNA was enriched from the total RNA using the PolyATract mRNA isolation system.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2012-09-28T17:01:38Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T15:46:01Z
attributes.tag: External Id
attributes.value: SAMEA1523636
attributes.tag: INSDC center name
attributes.value: Cambridge Research Institute Genomics Core Facility
attributes.tag: INSDC first public
attributes.value: 2012-09-28T17:01:38Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T15:46:01Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: StrainOrLine
attributes.value: CAST/EiJ x C57BL/6J
attributes.tag: Submitter Id
attributes.value: E-MTAB-1091:do928
attributes.tag: age
attributes.value: 4 to 6 month
attributes.tag: bio source type
attributes.value: frozen_sample
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: organism part
attributes.value: liver
attributes.tag: sample name
attributes.value: E-MTAB-1091:do928
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: male