title: Mutagenized HAP1 Tet-On-MLKLTE/SD cells treated with doxycyline
organism: Homo sapiens
description: Protocols: The selection experiment was performed by inducing MLKLTE/SD expression with 200 ng/mL DOX over five days and subsequently allowing resistant colonies to grow for four days after withdrawing DOX. Surviving colonies were collected using trypsin detachment. For the unselected sample cells were left untreated and cells were collected using trypsin. Nucleic acid was extracted using Qiagen columns ( QIAamp DNA Mini Kit; catalog # 51306) Nucleic acid library construction as performed as described before (PMID: 21623355)
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-05-16T17:03:08Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-05-11T10:09:32Z
attributes.tag: External Id
attributes.value: SAMEA4659243
attributes.tag: INSDC center name
attributes.value: STANFORD UNIVERSITY
attributes.tag: INSDC first public
attributes.value: 2018-05-16T17:03:08Z
attributes.tag: INSDC last update
attributes.value: 2018-05-11T10:09:32Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6777:Mutagenized HAP1 Tet-On-MLKLTE/SD cells treated with doxycyline
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: HAP1
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: chronic myelogenous leukemia
attributes.tag: genotype
attributes.value: Tet-On-MLKLTE/SD
attributes.tag: growth condition
attributes.value: selected
attributes.tag: sample name
attributes.value: E-MTAB-6777:Mutagenized HAP1 Tet-On-MLKLTE/SD cells treated with doxycyline
attributes.tag: scientific_name
attributes.value: Homo sapiens