title: Glomus irregulare DAOM 197198
organism: Rhizophagus irregularis DAOM 181602=DAOM 197198
description: This read set was one of four that was used to as a basis for the evolutionary network analysis in "Estimating genome diversity in arbuscular mycorrhizal fungi: the case for a pangenome" by Eva Boon, S*bastien Halary, Eric Bapteste and Mohamed Hijri. Approximately one million sterile spores of Glomus irregulare DAOM-197198 (commercial inoculant Mycorhise* ASP) were provided by Premier Tech Biotechnologies (Rivi*re-Du-Loup, QC, Canada) in a liquid suspension of 4000 spores per ml. This suspension was filtered on a sterile plastic 35 *m sieve. Under a binocular microscope, spores were checked for root contamination and root fragments were removed with forceps.  DNA was extracted using DNeasy Plant Mini kit (Qiagen), according to manufacturer's instructions. The purified DNA was then sent to the Genome Quebec Innovation Centre (McGill University, Montreal) for pyrosequencing using the GS FLX Titanium whole genome shotgun kit (Roche 454 Life Science), employing a full run for each DNA sample