title: CNTRLi_2in_1
organism: Mus musculus
description: Protocols: ES cells were maintained in Ndiff N2B27 media in the presence (ES conditions) of CHIR99021 (3uM) and PD032590 (1uM). Control RNAi, Oct4 RNAi or Otx2 RNAi, in the presence (ES conditions) or absence (Differentiation, 12hr) of CHIR99021 (3uM) and PD032590 (1uM). RNA was isolated using RNeasy plus mini Kit (QIAGEN) followed by DNaseI treatment (Ambion, Turbo DNA-free) and re-purify by Agencourt RNA clean XP beads (Beckman coulter) then stored at -80C SENSE mRNA-seq library Prep kit (Lexogen)
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2014-06-24T17:01:42Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T17:08:16Z
attributes.tag: External Id
attributes.value: SAMEA2336810
attributes.tag: INSDC center name
attributes.value: University of Manchester
attributes.tag: INSDC first public
attributes.value: 2014-06-24T17:01:42Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T17:08:16Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-2239:CNTRLi_2in_1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: E14Tg2a
attributes.tag: cell type
attributes.value: embryonic stem cell from blastocyst
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: embryo
attributes.tag: sample name
attributes.value: E-MTAB-2239:CNTRLi_2in_1
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: specimen with known storage state
attributes.value: fresh specimen