title: 6th time of Lachancea kluyveri S-phase time-course experiment
organism: Lachancea kluyveri NRRL Y-12651
description: G1 cells were isolated from an asynchronous population by centrifugal elutriation (Beckman: JE-5.0 Elutriator Rotor and Avanti J-26 XP centrifuge). Centrifugal elutriation is a technique for the separation of cells by velocity sedimentation (Pretlow et al., 1979). Asynchronous cells from an exponential culture in YPD were separated in different fractions. The fraction containing more than 95% of little G1 cells was centrifuged, washed and resuspend in YPD. Cells were synchronously released into S-phase at 23*C in YPD medium and one sample was taken every 10 minutes during 150 minutes. Genomic DNAs from 6 samples covering S phase (from 80 to 150 minutes) were extracted using the genomic-tip 20/G isolation kit (Qiagen).