title: IMR90_CD44_cNSF_HA_1
organism: Homo sapiens
description: Protocols: Cells were lysed in Trizol reagent. Cells were cultured in EMEM supplemented with 15% FBS. CD44- and empty-vector-overexpressing IMR90 cells were established by viral transduction and 1 μg/ml puromycin selection. Cells were then incubated for 6 h with PBS or Naked-mole rat skin fibroblasts-derived hyaluronan (cNSF-HA) (20ug/ml). The RNA samples were extracted using Trizol reagent. RNA samples were sent to the Psomagen, Inc. The TruSeq Stranded mRNA Kit (Illumina) was used for library construction.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-04-25T04:03:53Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-04-15T11:40:46Z
attributes.tag: External Id
attributes.value: SAMEA6678466
attributes.tag: INSDC center name
attributes.value: Department of Pathophysiology, Osaka City University Graduate School of Medicine
attributes.tag: INSDC first public
attributes.value: 2020-04-25T04:03:53Z
attributes.tag: INSDC last update
attributes.value: 2020-04-15T11:40:46Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8943:IMR90_CD44_cNSF_HA_1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: IMR-90
attributes.tag: cell type
attributes.value: fibroblast
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: late embryo stage
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: CD44 overexpression
attributes.tag: organism part
attributes.value: lung
attributes.tag: sample name
attributes.value: E-MTAB-8943:IMR90_CD44_cNSF_HA_1
attributes.tag: scientific_name
attributes.value: Homo sapiens