title: Snyder EGL-5 POL-2 L3 replicate 1
organism: Caenorhabditis elegans
attributes.tag: source_name
attributes.value: Snyder_EGL-5_POL-2_L3_rep1
attributes.tag: strain
attributes.value: OP54(official name : OP54 genotype : unc-119(ed3) III; wgIs54 [unc-119(+) egl-5::TY1::EGFP::3xFLAG] outcross : 3 mutagen : Bombard tags : GFP::3xFlag description : This strain's transgene was constructed by Mihail Sarov at the Max Planck Institute for Cell Biology in Tubiginen using Tony Hyman's recombineering pipeline. The resulting plasmid was used for biolistic transformation of an unc-119(ed3) strain. The EGL-5::EGFP fusion protein is expressed in the correct egl-5 spatio-temporal expression pattern. This strain was used for ChIP-seq experiments to map the in vivo binding sites for the EGL-5 transcription factor. made_by : R. Waterston and S. Kim )
attributes.tag: developmental stage
attributes.value: L3
attributes.tag: genotype
attributes.value: unc-119(ed3) III; wgIs54 [unc-119(+) egl-5::TY1::EGFP::3xFLAG]
attributes.tag: Sex
attributes.value: hermaphrodite