title: V5-PUF2 FT
organism: Trypanosoma brucei
description: Protocols: Cells were centrifuged out of the medium in vitro culture of bloodstream forms with standard HMI medium myc-tagged UPF1 was purified and RNA was prepared from the bound eluate and flow-thorough (FT) fractions RNAi was induced to deplete PUF2, +tet is induced and -tet is not induced RNA was made using Trifast reagent (Peqlab) according to the manufacturer's instructions illumina kit
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-05-21T17:01:59Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-05-11T16:23:31Z
attributes.tag: External Id
attributes.value: SAMEA4659730
attributes.tag: INSDC center name
attributes.value: ZMBH
attributes.tag: INSDC first public
attributes.value: 2018-05-21T17:01:59Z
attributes.tag: INSDC last update
attributes.value: 2018-05-11T16:23:31Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-5617:V5-PUF2 FT
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: developmental stage
attributes.value: bloodstream form
attributes.tag: genotype
attributes.value: V5-PUF2
attributes.tag: sample name
attributes.value: E-MTAB-5617:V5-PUF2 FT
attributes.tag: scientific_name
attributes.value: Trypanosoma brucei
attributes.tag: strain
attributes.value: Lister 427