title: D667
organism: Mus musculus
description: Protocols: Miz1 lox/lox mice were crossed with the desert hedgehog (Dhh)-Cre driver line (C57Bl6-Tg(Dhh- cre)1Mejr to achieve conditional ablation in Schwann cells of Miz1 exons 3 and 4, which encode the POZ domain (Sanz-Moreno et al. 2015). Mice had a mixed C57Bl6 and 129S2/SvHsd genetic background. Here, animals which were Dhh-Cre + ;Miz1 flox/flox are designated Miz∆POZ. Dissected sciatic nerve pairs of indicated ages were homogenized in TRI Reagent (Sigma, T9424). RNA was extracted, DNase treated (Macherey-Nagel, #740963), and purified using theNucleoSpin ® RNA Clean-up kit (Macherey-Nagel, #740948) according to the manufacturer's instructions. RNA from cell culture samples was extracted, DNase treated and isolated using theNucleoSpin® RNA kit (Macherey-Nagel, #740955). Sequencing libraries were prepared with the TruSeq Stranded mRNA Kit (Illumina).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-03-14T17:02:58Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2017-03-21T09:05:09Z
attributes.tag: External Id
attributes.value: SAMEA103926725
attributes.tag: INSDC center name
attributes.value: Institute of Molecular Oncology and Genomics Unit, Philipps University of Marburg
attributes.tag: INSDC first public
attributes.value: 2018-03-14T17:02:58Z
attributes.tag: INSDC last update
attributes.value: 2017-03-21T09:05:09Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-5600:D667
attributes.tag: age
attributes.value: 30
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: sciatic nerve
attributes.tag: phenotype
attributes.value: wild type
attributes.tag: sample name
attributes.value: E-MTAB-5600:D667
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: strain
attributes.value: mixed C57BL/6 and 129S2/SvHsd