title: UCI-9135402DT
organism: Homo sapiens
description: Protocols: 500 ng of DNA was used. Extension-ligation was performed according to manufacturer's protocol. Two PCR cycles were performed as follows: 95°C 3 minutes, followed by 2 cycles of 95°C for 30 seconds, 66°C for 30 seconds, and 72°C for 60 seconds. Following two cycles of PCR, Ampure Bead cleanup was carried out according to standard protocol and eluted in 25 uL. 20 uL of PCR product, 22 uL of PMM2/TDP1 and 4 uL of the i7 primer supplied with from the TruSeq Custom Amplicon Index Kit were added to PCR tubes, and PCR was carried out using the following conditions: 95°C for 30 seconds; 22 cycles of 95°C for 30 seconds, 66°C for 30 seconds, and 72°C for 60 seconds; and finally 72°C for 5 minutes
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2017-06-15T17:01:31Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2017-05-16T18:32:07Z
attributes.tag: External Id
attributes.value: SAMEA104030848
attributes.tag: INSDC center name
attributes.value: unspecified
attributes.tag: INSDC first public
attributes.value: 2017-06-15T17:01:31Z
attributes.tag: INSDC last update
attributes.value: 2017-05-16T18:32:07Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-5710:UCI-9135402DT
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: breast carcinoma
attributes.tag: individual
attributes.value: UCI-9135402
attributes.tag: organism part
attributes.value: breast
attributes.tag: sample name
attributes.value: E-MTAB-5710:UCI-9135402DT
attributes.tag: scientific_name
attributes.value: Homo sapiens