title: G166
organism: Homo sapiens
description: Protocols: GNS and NS cells were cultured in N2B27 serum-free medium, a 1:1 mixture of DMEM/F-12 and Neurobasal media (Invitrogen) augmented with N2 (Stem Cell Sciences) and B27 (Gibco) supplements. Self-renewal was supported by the addition of 10 ng/ml EGF and 20 ng/ml FGF-2 to the complete medium. Cells were plated at 20,000/cm2 in laminin-coated vessels (10 ug/ml laminin-1 (Sigma) in PBS for 6-12 h), passaged at confluence using Accutase dissociation reagent (Sigma) and were typically split at 1:3 for NS cells and 1:3 to 1:6 for GNS cells. TRIzol, phenol/chloroform extraction
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2012-09-24T17:00:57Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T15:38:24Z
attributes.tag: External Id
attributes.value: SAMEA1523617
attributes.tag: INSDC center name
attributes.value: MICHAEL SMITH GENOME SCIENCES CENTRE, VANCOUVER, CANADA
attributes.tag: INSDC first public
attributes.value: 2012-09-24T17:00:57Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T15:38:24Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-971:G166
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: primary cell line
attributes.tag: cell type
attributes.value: neuronal stem cell
attributes.tag: common name
attributes.value: human
attributes.tag: disease state
attributes.value: glioblastoma multiforme
attributes.tag: individual
attributes.value: G166
attributes.tag: sample name
attributes.value: E-MTAB-971:G166
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: female