title: Sample 6
organism: Mus musculus
description: Protocols: The full-length mRNA of mouse Klf1(NM-010635) was cloned into the pMIG retroviral vector, which contains an IRES-GFP cassette. Splenocytes from C57BL/6 mice were cultured for 48 h in the presence of Concanavalin A (ConA) (10 μg/mL) and IL-2 (50 μg/mL), and gene transduction was conducted. CD4+ T cells that were transfected with retroviral vectors were divided into 3 fractions according to GFP fluorescence (strongly positive, weakly positive and negative) using a FACSVantage flow cytometer. Total RNA was extracted using ｍRNA extraction kit(RNeasy Micro Kit, QIAGEN) Sequence libraries were prepared according to standard methods using Smart-seq2 (Illumina)
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-05-10T17:03:12Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-05-09T10:44:03Z
attributes.tag: External Id
attributes.value: SAMEA4653163
attributes.tag: INSDC center name
attributes.value: Department of Allergy and Rheumatology, Graduate School of Medicine, The University of Tokyo
attributes.tag: INSDC first public
attributes.value: 2018-05-10T17:03:12Z
attributes.tag: INSDC last update
attributes.value: 2018-05-09T10:44:03Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6778:Sample 6
attributes.tag: age
attributes.value: 8
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: CD4-positive helper T cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: pMIG-Klf1_GFP
attributes.tag: organism part
attributes.value: spleen
attributes.tag: phenotype
attributes.value: Klf1 overexpression; no GFP expression
attributes.tag: sample name
attributes.value: E-MTAB-6778:Sample 6
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: C57BL/6