title: PPD2-GSyellow_2
organism: Arabidopsis thaliana
description: Protocols: Cross-linking of Arabidopsis cell culture material was carried out as described (doi: 10.1104/pp.113.229617 Verkest et al., 2014). Cell suspension cultures were sub-cultured every 7 days by transfering 7 mL culture in 43 mL MSMO medium (containing per liter 4.43 g MSMO (minimal organics Sigma #M6899), 30g Sucrose, H2O, 50 μl kinetin (1 mg/ml stock solution) and 500 μl NAA (1mg/ml stock solution) and a pH adjusted to 5.7 with 1M KOH. Flasks were grown in the dark in an incubator at 25°C shaking at 130 rpm. ChIP-seq on PPD2-GSyellow was performed using 5 uL ab290 anti-GFP antibody (Abcam), with minor adaptations of a published protocol (Morohashi et al., 2012) and 1 g fixed material was used per ChIP. All subsequent steps were carried out as described by Morohashi et al. 2012. The PPD2-HBH TChAP-seq was performed using an Arabidopsis cell suspension culture overexpressing a HBH tagged PPD2 as described (doi: 10.1105/tpc.15.00006 Gonzalez et al., 2015) ChIP-seq libraries were prepared by GATC Biotech
attributes.tag: ENA first public
attributes.value: 2018-08-07
attributes.tag: ENA last update
attributes.value: 2018-02-16
attributes.tag: External Id
attributes.value: SAMEA104598816
attributes.tag: INSDC center alias
attributes.value: Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, Belgium VIB Center for Plant Systems Biology,VIB, Gent, Belgium
attributes.tag: INSDC center name
attributes.value: Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, Belgium VIB Center for Plant Systems Biology,VIB, Gent, Belgium
attributes.tag: INSDC first public
attributes.value: 2018-08-07T17:03:06Z
attributes.tag: INSDC last update
attributes.value: 2018-02-16T14:52:50Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6513:PPD2-GSyellow_2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: PSB-D
attributes.tag: common name
attributes.value: thale cress
attributes.tag: genotype
attributes.value: p35S:PPD2-GSyellow
attributes.tag: organism part
attributes.value: stem
attributes.tag: sample name
attributes.value: E-MTAB-6513:PPD2-GSyellow_2
attributes.tag: ecotype
attributes.value: Landsberg erecta