title: Cryoprotective Dehydration Sample 1
organism: Belgica antarctica
description: Larvae of B. antarctica were collected on offshore islands near Palmer Station (64*46’S, 64*04’W) in January, 2010. Larvae were extracted from their natural substrate into ice water using a modified Berlese apparatus, and concentrated samples of larvae were returned to natural substrate and shipped to Ohio State University at 4*C. The sample consisted of 20 larvae gradually chilled from -0.6 to -3*C at vapor pressure equilibrium with surrounding ice, then held at -3*C for 10 d, resulting in ~40% water loss.