title: HudsonAlpha_ChipSeq_GM12878_Egr-1_PCR2x
organism: Homo sapiens
attributes.tag: source_name
attributes.value: GM12878
attributes.tag: biomaterial_provider
attributes.value: Coriell; http://ccr.coriell.org/Sections/Search/Search.aspx?PgId=165&q=GM12878
attributes.tag: datatype
attributes.value: ChipSeq
attributes.tag: datatype description
attributes.value: Chromatin IP Sequencing
attributes.tag: antibody antibodydescription
attributes.value: Rabbit polyclonal, EGR-1 (588). Antibody Target: EGR1
attributes.tag: antibody targetdescription
attributes.value: Egr-1, Egr-2, Egr-3 and Egr-4 are nuclear transcription factors belonging to the Egr C2H2-type zinc-finger protein family and containing three C2H2-type zinc fingers. As immediate early proteins, Egr transcription factors are rapidly induced by diverse extracellular stimuli. Egr proteins are subject to tight differential control through diverse mechanisms at several levels of regulation including transcriptional, translational and post-translational (including glyco- sylation, phosphorylation and redox) mechanisms and protein-protein inter- action. Egr-1 binds to the DNA sequence 5'-CGCCCCCGC-3' (EGR-site), there- by activating transcription of target genes whose products are required for mitogenisis and differentiation. Egr-2 binds specific DNA sites located in the promoter region of HoxA4. Egr-2 defects cause congenital hypomyelination neuropathy (also designated Charcot-Marie-tooth disease) and Dejerine- Sottas neuropathology (also designated hereditary motor and sensory neuro- pathy III. Egr-3 is involved in muscle spindle development and is expressed in T cells 20 minutes following activation. EGR-4 binds to the EGR consensus motif GCGTGGGCG, functions as a transcriptional repressor, and displays autoregulatory activities, downregulating its on gene promoter in a dose dependent manner.
attributes.tag: antibody vendorname
attributes.value: Santa Cruz Biotechnology
attributes.tag: antibody vendorid
attributes.value: sc-110
attributes.tag: controlid
attributes.value: SL218
attributes.tag: labexpid
attributes.value: SL482
attributes.tag: softwareversion
attributes.value: MACS
attributes.tag: cell sex
attributes.value: F
attributes.tag: antibody
attributes.value: Egr-1
attributes.tag: antibody antibodydescription
attributes.value: Rabbit polyclonal, EGR-1 (588). Antibody Target: EGR1
attributes.tag: antibody targetdescription
attributes.value: Egr-1, Egr-2, Egr-3 and Egr-4 are nuclear transcription factors belonging to the Egr C2H2-type zinc-finger protein family and containing three C2H2-type zinc fingers. As immediate early proteins, Egr transcription factors are rapidly induced by diverse extracellular stimuli. Egr proteins are subject to tight differential control through diverse mechanisms at several levels of regulation including transcriptional, translational and post-translational (including glyco- sylation, phosphorylation and redox) mechanisms and protein-protein inter- action. Egr-1 binds to the DNA sequence 5'-CGCCCCCGC-3' (EGR-site), there- by activating transcription of target genes whose products are required for mitogenisis and differentiation. Egr-2 binds specific DNA sites located in the promoter region of HoxA4. Egr-2 defects cause congenital hypomyelination neuropathy (also designated Charcot-Marie-tooth disease) and Dejerine- Sottas neuropathology (also designated hereditary motor and sensory neuro- pathy III. Egr-3 is involved in muscle spindle development and is expressed in T cells 20 minutes following activation. EGR-4 binds to the EGR consensus motif GCGTGGGCG, functions as a transcriptional repressor, and displays autoregulatory activities, downregulating its on gene promoter in a dose dependent manner.
attributes.tag: antibody vendorname
attributes.value: Santa Cruz Biotechnology
attributes.tag: antibody vendorid
attributes.value: sc-110
attributes.tag: treatment
attributes.value: None
attributes.tag: treatment description
attributes.value: No special treatment or protocol applies
attributes.tag: protocol
attributes.value: PCR2x
attributes.tag: protocol description
attributes.value: a 25-cycle round of PCR and an additional 15-cycle round of PCR after gel size selection (Myers)
attributes.tag: controlid
attributes.value: SL218
attributes.tag: labexpid
attributes.value: SL482
attributes.tag: replicate
attributes.value: 3
attributes.tag: softwareversion
attributes.value: MACS