title: Control 112
organism: Rattus norvegicus
description: Protocols: At week 21 of the diet, animals were anesthetized with pentobarbital (60 mg/kg, i.p.), then hearts were excised, shortly perfused with oxygenated Krebs-Henseleit buffer in Langendorff mode and stored at -80°C in RNAlater. RNAlater-preserved rat left ventricle samples (n=6/group) were thawed on ice, then ca. 30 mg of left ventricular tissue were placed in a 1.5 ml Eppendorf LoBind tube containing glass beads (1.7-2.1 mm diameter, Carl Roth, Karlsruhe, Germany) and 500 µl of VRX buffer (Viogene Biotek, New Taipei City, Taiwan, R.O.C). The Eppendorf tube was firmly attached to a SILAMAT S5 vibrator (Ivoclar Vivadent, Schaan, Liechtenstein) in order to disrupt and homogenize the tissue for 15s. Total RNA was extracted using Viogene miTotal RNA Extraction Miniprep System (Viogene Biotek, New Taipei City, Taiwan, R.O.C) according to the manufacturer's protocol. For small RNA library construction, NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs, Ipswich, MA, USA) was applied according to the manufacturer's protocol. The quality and quantity of the library QC was performed by using High Sensitivity DNA1000 ScreenTape system with 2200 Tapestation (Agilent Technologies, Santa Clara, CA, USA) and dsDNA HS Assay Kit with Qubit 3.0 Fluorometer (Thermo Fisher Scientific, Waltham, MA, USA), respectively.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-04-24T17:05:07Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-08-01T12:04:30Z
attributes.tag: External Id
attributes.value: SAMEA5841976
attributes.tag: INSDC center name
attributes.value: Department of Pharmacology and Pharmacotherapy, Semmelweis University
attributes.tag: INSDC first public
attributes.value: 2020-04-24T17:05:07Z
attributes.tag: INSDC last update
attributes.value: 2019-08-01T12:04:30Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8204:Control 112
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: Norway rat
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: diet
attributes.value: normal chow
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: individual
attributes.value: 112
attributes.tag: mirna transcript levels
attributes.value: healthy profile
attributes.tag: organism part
attributes.value: myocardium of left ventricle
attributes.tag: sample name
attributes.value: E-MTAB-8204:Control 112
attributes.tag: scientific_name
attributes.value: Rattus norvegicus
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: Long-Evans