organism: Trypanosoma brucei brucei
attributes.tag: Alias
attributes.value: E-MTAB-1715_newsubmission:PL1S_1
attributes.tag: Broker name
attributes.value: ArrayExpress
attributes.tag: Description
attributes.value: Protocols: T. brucei bloodstream form parasites (strain Lister 427, antigenic type MiTat 1.2, clone 221a) [Johnson and Cross, 1979] were cultured at 37 degrees C, 5% CO2, in HMI-11 medium. RNA interference of histone H1 was induced for 4 days in culture. Total RNA was extracted using TRIzol standard protocol. Ribosomal RNA was depleted with RiboMinus kit (Invitrogen). PolyA-containing RNA was purified using Dynabeads oligo-(dT) (Invitrogen) and cDNA was synthesized using random hexamers according to Complete RNA-seq Library System kit (NuGEN) instructions.
attributes.tag: ENA checklist
attributes.value: ERC000011
attributes.tag: INSDC center alias
attributes.value: EBI
attributes.tag: INSDC center name
attributes.value: European Bioinformatics Institute
attributes.tag: INSDC first public
attributes.value: 2014-07-04T11:15:22Z
attributes.tag: INSDC last update
attributes.value: 2014-07-04T08:51:17Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: SRA accession
attributes.value: ERS497071
attributes.tag: Sample Name
attributes.value: ERS497071
attributes.tag: Title
attributes.value: PL1S_1
attributes.tag: developmental stage
attributes.value: bloodstream slender form
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism
attributes.value: Trypanosoma brucei brucei
attributes.tag: specimen with known storage state
attributes.value: fresh specimen
attributes.tag: strain
attributes.value: Lister 427
attributes.tag: sub_species
attributes.value: brucei