organism: marine metagenome
attributes.tag: isolation_source
attributes.value: Long Island Sound
attributes.tag: collection_date
attributes.value: 2008-05-22
attributes.tag: geo_loc_name
attributes.value: USA: Long Island Sound
attributes.tag: lat_lon
attributes.value: 40.88 N 73.33 W
attributes.tag: ref_biomaterial
attributes.value: Metatranscriptome profiling reveals versatile N-nutrient utilization, CO2 limitation, oxidative stress, and active toxin production in an Alexandrium fundyense bloom
attributes.tag: samp_collect_device
attributes.value: Filtration on polycarbonate membrane using vacuum pump
attributes.tag: samp_mat_process
attributes.value: A 0.3-0.9 L surface seawater for RNA analysis was filtered onto a 5-micrometer pore size (47mm diameter) polycarbonate membrane using a hand vacuum pump under low vacuum pressure. The filtration was completed within five minutes in order to capture the in situ gene expression profiles. The membrane was immersed in 1 mL TRIzol reagent (Invitrogen) and kept on ice until transport to the laboratory where the samples were kept at -80 Celsius until RNA extraction.
attributes.tag: samp_size
attributes.value: 0.3-0.9 L surface seawater
attributes.tag: source_material_id
attributes.value: LIS08
attributes.tag: BioSampleModel
attributes.value: Metagenome or environmental