organism: Solanum tuberosum
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: cultivar
attributes.value: not applicable
attributes.tag: ecotype
attributes.value: not applicable
attributes.tag: age
attributes.value: 110 days
attributes.tag: dev_stage
attributes.value: mature
attributes.tag: geo_loc_name
attributes.value: not collected
attributes.tag: tissue
attributes.value: tuber
attributes.tag: biomaterial_provider
attributes.value: Douches Lab, 1066 Bogue Street, East Lansing MI 48851
attributes.tag: collected_by
attributes.value: Kim Felcher
attributes.tag: collection_date
attributes.value: 2012-08-08
attributes.tag: genotype
attributes.value: Solanum tuberosum v. Tundra
attributes.tag: growth_protocol
attributes.value: Tissue culture plantlets of Kalkaska (R) and Tundra (S) were grown in 25 x 150 mm culture tubes on modified MS media at 23-27°C under fluorescent lights (30µmol·m-2·s-1) with a 16 h photoperiod. When roots were well established, plants were transferred to a soil-less, hydroponic nutrient film (NFT) system (Khatri, et al., 2010). During the first three weeks of growth in the NFT system, plants were fed with a 50% nutrient solution, pH 5.7 – 6.0 (IonicGrow, Hydrodynamics International, Lansing, MI) and maintained at a 16 h photoperiod under high-pressure sodium lights. For the remainder of the experiment, the photoperiod was reduced to 10 h to induce tuberization. Two days prior to treatment, tubers (3 to 5 mm in diameter) were lifted from the surface of the NFT system and placed in WhatmanTM Grade 1 filter-paper lined 100mm Petri dishes to prevent contamination of the system. Tubers used for the control treatment and tubers for thaxtomin A treatment were never on the same plant and plants were discarded after the tubers were harvested to prevent further use.
attributes.tag: lat_lon
attributes.value: 22.3 N 33.5 W
attributes.tag: phenotype
attributes.value: Common Scab Susceptible
attributes.tag: population
attributes.value: Parent of V507 Population
attributes.tag: sample_type
attributes.value: Tuber tissue
attributes.tag: treatment
attributes.value: Sterilized WhatmanTM Grade 1 filter paper discs (7mm) were created using a sterile hole punch and soaked in a control solution (2% ethanol) and air-dried. These treated discs were then placed onto the 3 tubers with sterile forceps. Tuber slices 2-3mm thick were taken from tissue directly beneath the treatment disc at 2, 4, 6, 8, 18, 20 and 24 h after treatment, immediately frozen in liquid nitrogen and stored at -80°C.
attributes.tag: BioSampleModel
attributes.value: Plant