organism: insect metagenome
attributes.tag: collection_date
attributes.value: 2005-07
attributes.tag: env_biome
attributes.value: Wet flatwoods
attributes.tag: env_feature
attributes.value: Longleaf pine
attributes.tag: env_material
attributes.value: tick
attributes.tag: geo_loc_name
attributes.value: USA:Tate's Hell, Florida
attributes.tag: host
attributes.value: Amblyomma maculatum
attributes.tag: lat_lon
attributes.value: 29.92 N 84.82 W
attributes.tag: host_life_stage
attributes.value: Adult
attributes.tag: host_sex
attributes.value: female
attributes.tag: host_taxid
attributes.value: 34609
attributes.tag: host_tissue_sampled
attributes.value: Half tick
attributes.tag: samp_collect_device
attributes.value: flagging
attributes.tag: samp_size
attributes.value: 1 individual tick
attributes.tag: samp_store_temp
attributes.value: -80 C
attributes.tag: nucl_acid_ext
attributes.value: Sequentially washed in 2% Micro-Chem Plus , 10.5% sodium hypochlorite, 3% hydrogen peroxide, and sterile water. Tick longitudinally bisected and 1 half flash frozen in liquid nitrogen, ground with a pestle, and extracted using the QIAamp DNA Mini-Kit (Qiagen, Valencia, CA).
attributes.tag: target_gene
attributes.value: 16S rDNA
attributes.tag: target_subfragment
attributes.value: V5-V3
attributes.tag: mid
attributes.value: ACGGCT
attributes.tag: pcr_primers
attributes.value: FWD:CCCGTCAATTCMTTTRAGT REV:CCTACGGGAGGCAGCAG
attributes.tag: seq_meth
attributes.value: pyrosequencing
attributes.tag: unique_seq_id
attributes.value: PTP776946:2:ACGGCT
attributes.tag: BioSampleModel
attributes.value: MIMARKS.survey
attributes.tag: BioSampleModel
attributes.value: MIGS/MIMS/MIMARKS.host-associated