organism: seawater metagenome
attributes.tag: isolation_source
attributes.value: not applicable
attributes.tag: collection_date
attributes.value: 2018-11-06
attributes.tag: geo_loc_name
attributes.value: not applicable
attributes.tag: lat_lon
attributes.value: not applicable
attributes.tag: samp_collect_device
attributes.value: Dry filter blank
attributes.tag: samp_mat_process
attributes.value: Sample was stored at -80C until processing. gDNA was extracted with the MasterPure Complete DNA and RNA Purification Kit (Lucigen). The 16S rRNA gene hypervariable regions V4-V5 were amplified with primers 515F-Y and 926R as recommended by Parada et al. (2016), multiplexed following the Earth Microbiome Project as in Caporaso et al. (2012). Amplicons were sequenced on an Illumina MiSeq using PE250 v2 chemistry.
attributes.tag: samp_size
attributes.value: n/a
attributes.tag: depth
attributes.value: not applicable
attributes.tag: eddy_state
attributes.value: not applicable
attributes.tag: experimental_treatment
attributes.value: Negative control 2
attributes.tag: BioSampleModel
attributes.value: Metagenome or environmental