organism: insect metagenome
attributes.tag: host
attributes.value: Drosophila melanogaster
attributes.tag: collection_date
attributes.value: 2017-06-30
attributes.tag: geo_loc_name
attributes.value: USA:Pennsylvania, Philadelphia
attributes.tag: env_broad_scale
attributes.value: orchard
attributes.tag: env_local_scale
attributes.value: cage 4, flies throughout cage and not at the diet, replicate 4
attributes.tag: env_medium
attributes.value: whole body homogenate
attributes.tag: lat_lon
attributes.value: 39.941960 N 75.202282 W
attributes.tag: host_age
attributes.value: variable from 0 to death
attributes.tag: host_body_habitat
attributes.value: whole body homogenate
attributes.tag: host_diet
attributes.value: 300 mL of diet in a diet pan. Diet recipe was: 1 L H2O, 11.6g agar, 147.5g corn meal, 20.2g soy flour, 34.9g yeast, 165ml molasses boiled and mixed together, then mixed with 1.25 L H2O. Separately, 4.9 g methyl paraben was dissolved in 99 ml ethanol and add to the diet after it was cooled.
attributes.tag: host_disease
attributes.value: not tested but expected to be healthy
attributes.tag: host_last_meal
attributes.value: not measured
attributes.tag: host_life_stage
attributes.value: adult
attributes.tag: host_sex
attributes.value: male
attributes.tag: host_substrate
attributes.value: 300 mL of diet in a diet pan. Diet recipe was: 1 L H2O, 11.6g agar, 147.5g corn meal, 20.2g soy flour, 34.9g yeast, 165ml molasses boiled and mixed together, then mixed with 1.25 L H2O. Separately, 4.9 g methyl paraben was dissolved in 99 ml ethanol and add to the diet after it was cooled.
attributes.tag: host_tissue_sampled
attributes.value: whole body homogenate
attributes.tag: organism_count
attributes.value: 5 whole body flies
attributes.tag: perturbation
attributes.value: diet inoculated with 2.2 ml OD600 0.1 Lactobacillus brevis DmCS_003 12-36 before allowing flies to feed on it. Diet was replaced 3 times weekly throughout the experiment
attributes.tag: BioSampleModel
attributes.value: MIMARKS.survey
attributes.tag: BioSampleModel
attributes.value: MIGS/MIMS/MIMARKS.host-associated