organism: soil metagenome
attributes.tag: isolation_source
attributes.value: soil
attributes.tag: collection_date
attributes.value: NA
attributes.tag: geo_loc_name
attributes.value: NA
attributes.tag: lat_lon
attributes.value: NA
attributes.tag: samp_collect_device
attributes.value: Metal core, 5.5 cm i.d., variable depth up to 13.5 cm
attributes.tag: samp_mat_process
attributes.value: Preserved in Lifeguard soil preservation solution, as cold as possible in field, then frozen, and finally -80°C
attributes.tag: samp_size
attributes.value: 250 mg
attributes.tag: Barcodes
attributes.value: CGTTGAAGGGATAAGG
attributes.tag: Sequencing_Sample_Name
attributes.value: 15S-NT-44M-1
attributes.tag: Project_ID
attributes.value: WB15
attributes.tag: Sample_ID
attributes.value: Blank-11
attributes.tag: Site_ID
attributes.value: Blank-11
attributes.tag: Soil_Extraction_Replicate
attributes.value: 11
attributes.tag: Org_or_Min
attributes.value: B
attributes.tag: Fwd_Primer_Barcode
attributes.value: CGTTGAAG
attributes.tag: Rev_Primer_Barcode
attributes.value: GGATAAGG
attributes.tag: Correct_ID
attributes.value: Blank-11
attributes.tag: env_biome
attributes.value: boreal
attributes.tag: env_material
attributes.value: soil
attributes.tag: env_package
attributes.value: mimarks
attributes.tag: nucl_acid_ext
attributes.value: Qiagen / MoBio PowerLyzer PowerSoil DNA extraction kit
attributes.tag: target_gene
attributes.value: 16S rRNA
attributes.tag: target_subfragment
attributes.value: V4
attributes.tag: pcr_primers
attributes.value: FWD: AATGATACGGCGACCACCGAGATCTACACNNNNNNNNTATGGTAATTGTGTGYCAGCMGCCGCGGTAA REV: CAAGCAGAAGACGGCATACGAGATNNNNNNNNAGTCAGCCAGCCGGACTACNVGGGTWTCTAAT
attributes.tag: pcr_cond
attributes.value: initial denaturation:98_2; denaturation:98_0.167; annealing:58_0.25; elongation:72_0.167; final elongation:72_2; total cycles: 30
attributes.tag: seq_meth
attributes.value: Illumina MiSeq 2x250 PE
attributes.tag: BioSampleModel
attributes.value: Metagenome or environmental