organism: Mus musculus
attributes.tag: ArrayExpress-CellType
attributes.value: TS-Rs26
attributes.tag: ArrayExpress-Species
attributes.value: Mus musculus
attributes.tag: ENA first public
attributes.value: 2017-01-24
attributes.tag: ENA last update
attributes.value: 2018-02-08
attributes.tag: External Id
attributes.value: SAMEA4415625
attributes.tag: INSDC center alias
attributes.value: SC
attributes.tag: INSDC center name
attributes.value: Wellcome Sanger Institute
attributes.tag: INSDC first public
attributes.value: 2017-01-24T13:33:27Z
attributes.tag: INSDC last update
attributes.value: 2018-02-08T16:43:59Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: 1995d8b0-4d97-11e6-9a3d-3c4a9275d6c8
attributes.tag: common name
attributes.value: house mouse
attributes.tag: sample description
attributes.value: Total RNA from a single confirmed Crb2 knockout clone generated and grown in parallel to vector control clones was extracted using TRIzol reagent. Cells were plated at 20,000 cells/6-well well. The RNA sample is a pool of 2 6-well wells grown in parallel.Total RNA from a single vector control clone generated and grown in parallel to Crb2 HOMs clones was prepared as described above. Cells were plated at 20,000 cells/6-well well. The RNA sample is a pool of 2 6-well wells grown in parallel. Cells were plated in TSC conditions, media changed to Differentiation conditions the next day, and cells harvested 3 days later, i.e. 3D of differentiation. The Crb2 CRISPR knockout design deletes exons 7&8.
attributes.tag: sample name
attributes.value: 1995d8b0-4d97-11e6-9a3d-3c4a9275d6c8