organism: soil metagenome
attributes.tag: isolation_source
attributes.value: soil
attributes.tag: collection_date
attributes.value: 2015-07-24
attributes.tag: geo_loc_name
attributes.value: Canada: Northwest Territories
attributes.tag: lat_lon
attributes.value: 62.5409 N 113.1353 W
attributes.tag: samp_collect_device
attributes.value: Metal core, 5.5 cm i.d., variable depth up to 13.5 cm
attributes.tag: samp_mat_process
attributes.value: Preserved in Lifeguard soil preservation solution, as cold as possible in field, then frozen, and finally -80°C
attributes.tag: samp_size
attributes.value: 250 mg
attributes.tag: Barcodes
attributes.value: CGCTATTAACGCATTC
attributes.tag: Sequencing_Sample_Name
attributes.value: 15S-NT-48O-2
attributes.tag: Project_ID
attributes.value: WB15
attributes.tag: Sample_ID
attributes.value: 15S-NT-47O
attributes.tag: Site_ID
attributes.value: 15S-NT-47
attributes.tag: Soil_Extraction_Replicate
attributes.value: 1
attributes.tag: Org_or_Min
attributes.value: O
attributes.tag: Fwd_Primer_Barcode
attributes.value: CGCTATTA
attributes.tag: Rev_Primer_Barcode
attributes.value: ACGCATTC
attributes.tag: Exch_Na_mg_kg
attributes.value: 0
attributes.tag: Exch_Mg_mg_kg
attributes.value: 0
attributes.tag: Exch_K_mg_kg
attributes.value: 0
attributes.tag: Exch_Ca_mg_kg
attributes.value: 0
attributes.tag: pH
attributes.value: 5.73
attributes.tag: EC_mS_cm
attributes.value: 0.422
attributes.tag: CEC_cmol_kg
attributes.value: 0
attributes.tag: Total_N_pct
attributes.value: 1.03
attributes.tag: Total_S_pct
attributes.value: 0
attributes.tag: Sand_pct
attributes.value: 0
attributes.tag: Clay_pct
attributes.value: 0
attributes.tag: Silt_pct
attributes.value: 0
attributes.tag: Ca_mg_kg
attributes.value: 17947.5
attributes.tag: K_mg_kg
attributes.value: 982.217
attributes.tag: Mg_mg_kg
attributes.value: 2319.02
attributes.tag: Na_mg_kg
attributes.value: 141.795
attributes.tag: P_mg_kg
attributes.value: 1048.48
attributes.tag: S_mg_kg
attributes.value: 1414.47
attributes.tag: Al_mg_kg
attributes.value: 2105.77
attributes.tag: Fe_mg_kg
attributes.value: 2652.94
attributes.tag: Zn_mg_kg
attributes.value: 11.873
attributes.tag: Cu_mg_kg
attributes.value: 99.741
attributes.tag: Mn_mg_kg
attributes.value: 1762.91
attributes.tag: Mo_mg_kg
attributes.value: 51.67
attributes.tag: TIC_ash_pct
attributes.value: 7.59
attributes.tag: TOC_LOI_pct
attributes.value: 52.0368
attributes.tag: TOC_HCL_cruc_pct
attributes.value: 50.1
attributes.tag: TC_pct
attributes.value: 48.5
attributes.tag: Fire_ID
attributes.value: 201ZF-017
attributes.tag: Ecosite
attributes.value: e2.1
attributes.tag: Moisture
attributes.value: Subhygric
attributes.tag: Moisture_Regime
attributes.value: 6
attributes.tag: Nutrient
attributes.value: Rich
attributes.tag: Community
attributes.value: Aw-Sw-Sb/willow/horsetail
attributes.tag: Forest
attributes.value: 1
attributes.tag: Plains
attributes.value: 0
attributes.tag: Interval
attributes.value: Short
attributes.tag: Veg_Comm
attributes.value: Black Spruce
attributes.tag: Land_Class_Unburned
attributes.value: Upland
attributes.tag: Land_Class
attributes.value: Upland
attributes.tag: Burned_Unburned
attributes.value: Burned
attributes.tag: Understory_CBI
attributes.value: 1.85
attributes.tag: Overstory_CBI
attributes.value: 3
attributes.tag: CBI
attributes.value: 2.31
attributes.tag: RBR
attributes.value: 566.2085162
attributes.tag: Pct_Exposed_Mineral
attributes.value: 2.039473684
attributes.tag: CFSI
attributes.value: 5.145
attributes.tag: Burn_Severity_Index
attributes.value: 3.621710526
attributes.tag: Mean_Duff_Depth_cm
attributes.value: 7.625
attributes.tag: O_Depth_cm
attributes.value: 5
attributes.tag: Dead_Trees
attributes.value: 32
attributes.tag: Live_Trees
attributes.value: 0
attributes.tag: nTrees
attributes.value: 32
attributes.tag: Severity_Class
attributes.value: High
attributes.tag: temp
attributes.value: 25.49155997
attributes.tag: rh
attributes.value: 34.27665305
attributes.tag: ws
attributes.value: 15.22764651
attributes.tag: prec
attributes.value: 0.04224861
attributes.tag: ffmc
attributes.value: 91.01891405
attributes.tag: dmc
attributes.value: 88.39637387
attributes.tag: dc
attributes.value: 380.9300135
attributes.tag: isi
attributes.value: 10.68205565
attributes.tag: bui
attributes.value: 111.8845656
attributes.tag: fwi
attributes.value: 34.6073629
attributes.tag: Correct_ID
attributes.value: 15S-NT-47O-1
attributes.tag: Number_Fires_Recorded
attributes.value: 3
attributes.tag: TSLF
attributes.value: 62
attributes.tag: TSO
attributes.value: 81
attributes.tag: Fires_PerYear
attributes.value: 0.037037037
attributes.tag: Fires_PerDecade_Recording
attributes.value: 0.333333333
attributes.tag: Longest_Period_Btw_Fires
attributes.value: 61
attributes.tag: Shortest_Period_Btw_Fires
attributes.value: 18
attributes.tag: Mean_Period_Btw_Fires
attributes.value: 39.5
attributes.tag: Reburn_Severity_Index
attributes.value: 1.00152439
attributes.tag: Overstory_Dens_ha
attributes.value: 4818.347363
attributes.tag: Understory_Dens_ha
attributes.value: 7427.23067
attributes.tag: Abs_Density_Ha_UnderOver
attributes.value: 12245.57803
attributes.tag: PINUBAN_Stems_Ha
attributes.value: 0
attributes.tag: PICEMAR_Stems_Ha
attributes.value: 4216.053943
attributes.tag: PICEGLA_Stems_Ha
attributes.value: 0
attributes.tag: LARILAR_Stems_Ha
attributes.value: 0
attributes.tag: POPUTRE_Stems_Ha
attributes.value: 0
attributes.tag: POPUBAL_Stems_Ha
attributes.value: 0
attributes.tag: BETUPAP_Stems_Ha
attributes.value: 150.5733551
attributes.tag: PINUBAN_BA_per_Ha
attributes.value: 0
attributes.tag: PICEMAR_BA_per_Ha
attributes.value: 9.938218692
attributes.tag: PICEGLA_BA_per_Ha
attributes.value: 0
attributes.tag: LARILAR_BA_per_Ha
attributes.value: 0
attributes.tag: POPUTRE_BA_per_Ha
attributes.value: 0
attributes.tag: POPUBAL_BA_per_Ha
attributes.value: 0
attributes.tag: BETUPAP_BA_per_Ha
attributes.value: 0.834442818
attributes.tag: BA_ha
attributes.value: 10.77266151
attributes.tag: Pct_Conifer_Stems_lar
attributes.value: 96.55172414
attributes.tag: Pct_Conifer_BA_lar
attributes.value: 92.25407002
attributes.tag: Median_CBH
attributes.value: 5.1
attributes.tag: Flammable_T_ha
attributes.value: 9.756324908
attributes.tag: Conifer_CBD
attributes.value: 0.993445755
attributes.tag: Surface_Severity_Class
attributes.value: High
attributes.tag: Soil_Class
attributes.value: Typic Mesisol
attributes.tag: env_biome
attributes.value: boreal
attributes.tag: env_feature
attributes.value: Burned Upland Black Spruce
attributes.tag: env_material
attributes.value: soil
attributes.tag: env_package
attributes.value: mimarks
attributes.tag: nucl_acid_ext
attributes.value: Qiagen / MoBio PowerLyzer PowerSoil DNA extraction kit
attributes.tag: target_gene
attributes.value: 16S rRNA
attributes.tag: target_subfragment
attributes.value: V4
attributes.tag: pcr_primers
attributes.value: FWD: AATGATACGGCGACCACCGAGATCTACACNNNNNNNNTATGGTAATTGTGTGYCAGCMGCCGCGGTAA REV: CAAGCAGAAGACGGCATACGAGATNNNNNNNNAGTCAGCCAGCCGGACTACNVGGGTWTCTAAT
attributes.tag: pcr_cond
attributes.value: initial denaturation:98_2; denaturation:98_0.167; annealing:58_0.25; elongation:72_0.167; final elongation:72_2; total cycles: 30
attributes.tag: seq_meth
attributes.value: Illumina MiSeq 2x250 PE
attributes.tag: BioSampleModel
attributes.value: Metagenome or environmental