organism: soil metagenome
attributes.tag: isolation_source
attributes.value: soil
attributes.tag: collection_date
attributes.value: 2015-07-03
attributes.tag: geo_loc_name
attributes.value: Canada: Northwest Territories
attributes.tag: lat_lon
attributes.value: 62.5097 N 116.4883 W
attributes.tag: samp_collect_device
attributes.value: Metal core, 5.5 cm i.d., variable depth up to 13.5 cm
attributes.tag: samp_mat_process
attributes.value: Preserved in Lifeguard soil preservation solution, as cold as possible in field, then frozen, and finally -80°C
attributes.tag: samp_size
attributes.value: 250 mg
attributes.tag: Barcodes
attributes.value: CGCTATTAAGAGACGT
attributes.tag: Sequencing_Sample_Name
attributes.value: 15S-NT-20O-B-2
attributes.tag: Project_ID
attributes.value: WB15
attributes.tag: Sample_ID
attributes.value: 15S-NT-20O
attributes.tag: Site_ID
attributes.value: 15S-NT-20
attributes.tag: Soil_Extraction_Replicate
attributes.value: 1
attributes.tag: Org_or_Min
attributes.value: O
attributes.tag: Fwd_Primer_Barcode
attributes.value: CGCTATTA
attributes.tag: Rev_Primer_Barcode
attributes.value: AGAGACGT
attributes.tag: Fire_ID
attributes.value: 2014ZF-046
attributes.tag: Ecosite
attributes.value: j2.1
attributes.tag: Moisture
attributes.value: Subhydric
attributes.tag: Moisture_Regime
attributes.value: 8
attributes.tag: Nutrient
attributes.value: Medium
attributes.tag: Community
attributes.value: willow/golden moss
attributes.tag: Forest
attributes.value: 0
attributes.tag: Plains
attributes.value: 1
attributes.tag: Interval
attributes.value: No Trees
attributes.tag: Veg_Comm
attributes.value: Open Wetland
attributes.tag: Land_Class_Unburned
attributes.value: Wetland
attributes.tag: Land_Class
attributes.value: Wetland
attributes.tag: Burned_Unburned
attributes.value: Burned
attributes.tag: Understory_CBI
attributes.value: 1.05
attributes.tag: Overstory_CBI
attributes.value: NA
attributes.tag: CBI
attributes.value: 1.05
attributes.tag: RBR
attributes.value: 325.1928954
attributes.tag: Pct_Exposed_Mineral
attributes.value: 0
attributes.tag: CFSI
attributes.value: 1
attributes.tag: Burn_Severity_Index
attributes.value: 2.375
attributes.tag: Mean_Duff_Depth_cm
attributes.value: 10
attributes.tag: O_Depth_cm
attributes.value: 8.5
attributes.tag: Dead_Trees
attributes.value: 8
attributes.tag: Live_Trees
attributes.value: 0
attributes.tag: nTrees
attributes.value: 8
attributes.tag: Severity_Class
attributes.value: Low
attributes.tag: temp
attributes.value: 28.36350358
attributes.tag: rh
attributes.value: 33.72715235
attributes.tag: ws
attributes.value: 11.95882987
attributes.tag: prec
attributes.value: 3.45e-05
attributes.tag: ffmc
attributes.value: 91.38864948
attributes.tag: dmc
attributes.value: 96.34697394
attributes.tag: dc
attributes.value: 556.4435862
attributes.tag: isi
attributes.value: 9.549219773
attributes.tag: bui
attributes.value: 134.4811605
attributes.tag: fwi
attributes.value: 34.47629061
attributes.tag: Correct_ID
attributes.value: 15S-NT-20O-A-1
attributes.tag: Number_Fires_Recorded
attributes.value: 2
attributes.tag: TSLF
attributes.value: 42
attributes.tag: TSO
attributes.value: 42
attributes.tag: Fires_PerYear
attributes.value: 0.047619048
attributes.tag: Fires_PerDecade_Recording
attributes.value: 0.4
attributes.tag: Longest_Period_Btw_Fires
attributes.value: 42
attributes.tag: Shortest_Period_Btw_Fires
attributes.value: 42
attributes.tag: Mean_Period_Btw_Fires
attributes.value: 42
attributes.tag: Reburn_Severity_Index
attributes.value: 0.999726402
attributes.tag: Overstory_Dens_ha
attributes.value: 32.24983327
attributes.tag: Understory_Dens_ha
attributes.value: 176.83883
attributes.tag: Abs_Density_Ha_UnderOver
attributes.value: 209.0886633
attributes.tag: PINUBAN_Stems_Ha
attributes.value: 32.24983327
attributes.tag: PICEMAR_Stems_Ha
attributes.value: 0
attributes.tag: PICEGLA_Stems_Ha
attributes.value: 0
attributes.tag: LARILAR_Stems_Ha
attributes.value: 0
attributes.tag: POPUTRE_Stems_Ha
attributes.value: 0
attributes.tag: POPUBAL_Stems_Ha
attributes.value: 0
attributes.tag: BETUPAP_Stems_Ha
attributes.value: 0
attributes.tag: PINUBAN_BA_per_Ha
attributes.value: 0.13210319
attributes.tag: PICEMAR_BA_per_Ha
attributes.value: 0
attributes.tag: PICEGLA_BA_per_Ha
attributes.value: 0
attributes.tag: LARILAR_BA_per_Ha
attributes.value: 0
attributes.tag: POPUTRE_BA_per_Ha
attributes.value: 0
attributes.tag: POPUBAL_BA_per_Ha
attributes.value: 0
attributes.tag: BETUPAP_BA_per_Ha
attributes.value: 0
attributes.tag: BA_ha
attributes.value: 0.13210319
attributes.tag: Pct_Conifer_Stems_lar
attributes.value: 100
attributes.tag: Pct_Conifer_BA_lar
attributes.value: 100
attributes.tag: Median_CBH
attributes.value: 1
attributes.tag: Flammable_T_ha
attributes.value: 0.249652941
attributes.tag: Conifer_CBD
attributes.value: 0
attributes.tag: Surface_Severity_Class
attributes.value: Mixed
attributes.tag: Soil_Class
attributes.value: Typic Mesisol
attributes.tag: env_biome
attributes.value: boreal
attributes.tag: env_feature
attributes.value: Burned Wetland Open Wetland
attributes.tag: env_material
attributes.value: soil
attributes.tag: env_package
attributes.value: mimarks
attributes.tag: nucl_acid_ext
attributes.value: Qiagen / MoBio PowerLyzer PowerSoil DNA extraction kit
attributes.tag: target_gene
attributes.value: rRNA gene operon
attributes.tag: target_subfragment
attributes.value: ITS2
attributes.tag: pcr_primers
attributes.value: FWD: AATGATACGGCGACCACCGAGATCTACACNNNNNNNNTATGGTAATTAAAGCCTCCGCTTATTGATATGCTTAART REV: CAAGCAGAAGACGGCATACGAGATNNNNNNNNAGTCAGTCAGGGAACTTTYRRCAAYGGATCWCT
attributes.tag: pcr_cond
attributes.value: initial denaturation:98_2; denaturation:98_0.167; annealing:57_0.25; elongation:72_0.167; final elongation:72_2; total cycles: 40
attributes.tag: seq_meth
attributes.value: Illumina MiSeq 2x300 PE
attributes.tag: BioSampleModel
attributes.value: Metagenome or environmental