organism: seawater metagenome
attributes.tag: isolation_source
attributes.value: seawater
attributes.tag: collection_date
attributes.value: 2018-04-05
attributes.tag: geo_loc_name
attributes.value: Pacific Ocean: North Pacific Subtropical Gyre
attributes.tag: lat_lon
attributes.value: 22.3553 N 157.7282 W
attributes.tag: samp_collect_device
attributes.value: Water collected with Niskin bottle; filtered under low peristaltic pressure onto a 25 mm, 0.2 m pore size polyethersulfone filter (Supor)
attributes.tag: samp_mat_process
attributes.value: Sample was stored at -80C until processing. gDNA was extracted with the MasterPure Complete DNA and RNA Purification Kit (Lucigen). The 16S rRNA gene hypervariable regions V4-V5 were amplified with primers 515F-Y and 926R as recommended by Parada et al. (2016), multiplexed following the Earth Microbiome Project as in Caporaso et al. (2012). Amplicons were sequenced on an Illumina MiSeq using PE250 v2 chemistry.
attributes.tag: samp_size
attributes.value: ~1L
attributes.tag: depth
attributes.value: 75
attributes.tag: eddy_state
attributes.value: Cyclone - edge
attributes.tag: experimental_treatment
attributes.value: Field
attributes.tag: BioSampleModel
attributes.value: Metagenome or environmental