organism: Mus musculus
attributes.tag: strain
attributes.value: BALB/c
attributes.tag: dev_stage
attributes.value: Tumor sizes were measured twice weekly, unblended, until they reached a volume of 2000 mm3 or a diameter of 2 cm upon which the animal was euthanized.
attributes.tag: sex
attributes.value: female
attributes.tag: tissue
attributes.value: osteosarcoma tumor
attributes.tag: cell_line
attributes.value: K7M2
attributes.tag: disease
attributes.value: osteosarcoma
attributes.tag: growth_protocol
attributes.value: All murine osteosarcoma cell lines were maintained in RPMI 1640 supplemented with 15% heat-inactivated fetal bovine serum (FBS), 1% L-glutamine, 1% nonessential amino acids, 50 M 2-metacaptoethanol, 100 IU/mL penicillin, and 100 g/mL streptomycin. Vero cells were obtained from the American Type Culture Collection (Manassas, VA) and cultured in DMEM supplemented with 10% FBS, 100 IU/mL penicillin, and 100 g/mL streptomycin. K7M2 tumors were established by transplanting small pieces of sectioned tumor (~2mm3) into the flanks of 6-week-old Balb/C mice (Envigo).
attributes.tag: BioSampleModel
attributes.value: Model organism or animal