organism: soil metagenome
attributes.tag: isolation_source
attributes.value: soil
attributes.tag: collection_date
attributes.value: 2015-07-27
attributes.tag: geo_loc_name
attributes.value: Canada: Northwest Territories
attributes.tag: lat_lon
attributes.value: 61.7777 N 117.1704 W
attributes.tag: samp_collect_device
attributes.value: Metal core, 5.5 cm i.d., variable depth up to 13.5 cm
attributes.tag: samp_mat_process
attributes.value: Preserved in Lifeguard soil preservation solution, as cold as possible in field, then frozen, and finally -80°C
attributes.tag: samp_size
attributes.value: 250 mg
attributes.tag: Barcodes
attributes.value: GTTGCCAACTTAGCAG
attributes.tag: Sequencing_Sample_Name
attributes.value: 15S-NT-51O-1
attributes.tag: Project_ID
attributes.value: WB15
attributes.tag: Sample_ID
attributes.value: 15S-NT-49O
attributes.tag: Site_ID
attributes.value: 15S-NT-49
attributes.tag: Soil_Extraction_Replicate
attributes.value: 2
attributes.tag: Org_or_Min
attributes.value: O
attributes.tag: Fwd_Primer_Barcode
attributes.value: GTTGCCAA
attributes.tag: Rev_Primer_Barcode
attributes.value: CTTAGCAG
attributes.tag: Exch_Na_mg_kg
attributes.value: 0
attributes.tag: Exch_Mg_mg_kg
attributes.value: 0
attributes.tag: Exch_K_mg_kg
attributes.value: 0
attributes.tag: Exch_Ca_mg_kg
attributes.value: 0
attributes.tag: pH
attributes.value: 6.81
attributes.tag: EC_mS_cm
attributes.value: 1.252
attributes.tag: CEC_cmol_kg
attributes.value: 0
attributes.tag: Total_N_pct
attributes.value: 1.38
attributes.tag: Total_S_pct
attributes.value: 0
attributes.tag: Sand_pct
attributes.value: 0
attributes.tag: Clay_pct
attributes.value: 0
attributes.tag: Silt_pct
attributes.value: 0
attributes.tag: Ca_mg_kg
attributes.value: 32936.2
attributes.tag: K_mg_kg
attributes.value: 523.796
attributes.tag: Mg_mg_kg
attributes.value: 5158.73
attributes.tag: Na_mg_kg
attributes.value: 414.018
attributes.tag: P_mg_kg
attributes.value: 719.351
attributes.tag: S_mg_kg
attributes.value: 8679.42
attributes.tag: Al_mg_kg
attributes.value: 2199.82
attributes.tag: Fe_mg_kg
attributes.value: 5506.82
attributes.tag: Zn_mg_kg
attributes.value: < DL
attributes.tag: Cu_mg_kg
attributes.value: 69.44
attributes.tag: Mn_mg_kg
attributes.value: 379.208
attributes.tag: Mo_mg_kg
attributes.value: 59.049
attributes.tag: TIC_ash_pct
attributes.value: 9.34
attributes.tag: TOC_LOI_pct
attributes.value: 46.5299
attributes.tag: TOC_HCL_cruc_pct
attributes.value: 45
attributes.tag: TC_pct
attributes.value: 42.1
attributes.tag: Fire_ID
attributes.value: 2014ZF-020
attributes.tag: Ecosite
attributes.value: g1.1
attributes.tag: Moisture
attributes.value: Subhydric
attributes.tag: Moisture_Regime
attributes.value: 8
attributes.tag: Nutrient
attributes.value: Medium
attributes.tag: Community
attributes.value: Sb-Pj/Labrador tea/feather moss
attributes.tag: Forest
attributes.value: 1
attributes.tag: Plains
attributes.value: 1
attributes.tag: Interval
attributes.value: Moderate
attributes.tag: Veg_Comm
attributes.value: Black Spruce
attributes.tag: Land_Class_Unburned
attributes.value: Upland
attributes.tag: Land_Class
attributes.value: Upland
attributes.tag: Burned_Unburned
attributes.value: Burned
attributes.tag: Understory_CBI
attributes.value: 1.51
attributes.tag: Overstory_CBI
attributes.value: 3
attributes.tag: CBI
attributes.value: 2.11
attributes.tag: RBR
attributes.value: 550.4300349
attributes.tag: Pct_Exposed_Mineral
attributes.value: 1.75
attributes.tag: CFSI
attributes.value: 5.025
attributes.tag: Burn_Severity_Index
attributes.value: 3.09
attributes.tag: Mean_Duff_Depth_cm
attributes.value: 9.5
attributes.tag: O_Depth_cm
attributes.value: 7.5
attributes.tag: Dead_Trees
attributes.value: 32
attributes.tag: Live_Trees
attributes.value: 0
attributes.tag: nTrees
attributes.value: 32
attributes.tag: Severity_Class
attributes.value: Moderate
attributes.tag: temp
attributes.value: 30.91980194
attributes.tag: rh
attributes.value: 33.72637045
attributes.tag: ws
attributes.value: 10.91278365
attributes.tag: prec
attributes.value: 0.011632356
attributes.tag: ffmc
attributes.value: 92.20561449
attributes.tag: dmc
attributes.value: 59.05081016
attributes.tag: dc
attributes.value: 476.4443183
attributes.tag: isi
attributes.value: 10.17018563
attributes.tag: bui
attributes.value: 90.16412394
attributes.tag: fwi
attributes.value: 30.30663896
attributes.tag: Correct_ID
attributes.value: 15S-NT-49O-2
attributes.tag: Number_Fires_Recorded
attributes.value: 2
attributes.tag: TSLF
attributes.value: 103
attributes.tag: TSO
attributes.value: 103
attributes.tag: Fires_PerYear
attributes.value: 0.019417476
attributes.tag: Fires_PerDecade_Recording
attributes.value: 0.181818182
attributes.tag: Longest_Period_Btw_Fires
attributes.value: 102
attributes.tag: Shortest_Period_Btw_Fires
attributes.value: 102
attributes.tag: Mean_Period_Btw_Fires
attributes.value: 102
attributes.tag: Reburn_Severity_Index
attributes.value: 0.999953549
attributes.tag: Overstory_Dens_ha
attributes.value: 4162.330905
attributes.tag: Understory_Dens_ha
attributes.value: 7780.90833
attributes.tag: Abs_Density_Ha_UnderOver
attributes.value: 11943.23924
attributes.tag: PINUBAN_Stems_Ha
attributes.value: 0
attributes.tag: PICEMAR_Stems_Ha
attributes.value: 3381.893861
attributes.tag: PICEGLA_Stems_Ha
attributes.value: 520.2913632
attributes.tag: LARILAR_Stems_Ha
attributes.value: 0
attributes.tag: POPUTRE_Stems_Ha
attributes.value: 0
attributes.tag: POPUBAL_Stems_Ha
attributes.value: 0
attributes.tag: BETUPAP_Stems_Ha
attributes.value: 0
attributes.tag: PINUBAN_BA_per_Ha
attributes.value: 0
attributes.tag: PICEMAR_BA_per_Ha
attributes.value: 4.607778195
attributes.tag: PICEGLA_BA_per_Ha
attributes.value: 0.84230071
attributes.tag: LARILAR_BA_per_Ha
attributes.value: 0
attributes.tag: POPUTRE_BA_per_Ha
attributes.value: 0
attributes.tag: POPUBAL_BA_per_Ha
attributes.value: 0
attributes.tag: BETUPAP_BA_per_Ha
attributes.value: 0
attributes.tag: BA_ha
attributes.value: 5.450078905
attributes.tag: Pct_Conifer_Stems_lar
attributes.value: 100
attributes.tag: Pct_Conifer_BA_lar
attributes.value: 100
attributes.tag: Median_CBH
attributes.value: 3.3
attributes.tag: Flammable_T_ha
attributes.value: 6.075469358
attributes.tag: Conifer_CBD
attributes.value: 0.646107149
attributes.tag: Surface_Severity_Class
attributes.value: Mixed
attributes.tag: Soil_Class
attributes.value: Orthic Gleysol
attributes.tag: env_biome
attributes.value: boreal
attributes.tag: env_feature
attributes.value: Burned Upland Black Spruce
attributes.tag: env_material
attributes.value: soil
attributes.tag: env_package
attributes.value: mimarks
attributes.tag: nucl_acid_ext
attributes.value: Qiagen / MoBio PowerLyzer PowerSoil DNA extraction kit
attributes.tag: target_gene
attributes.value: rRNA gene operon
attributes.tag: target_subfragment
attributes.value: ITS2
attributes.tag: pcr_primers
attributes.value: FWD: AATGATACGGCGACCACCGAGATCTACACNNNNNNNNTATGGTAATTAAAGCCTCCGCTTATTGATATGCTTAART REV: CAAGCAGAAGACGGCATACGAGATNNNNNNNNAGTCAGTCAGGGAACTTTYRRCAAYGGATCWCT
attributes.tag: pcr_cond
attributes.value: initial denaturation:98_2; denaturation:98_0.167; annealing:57_0.25; elongation:72_0.167; final elongation:72_2; total cycles: 30
attributes.tag: seq_meth
attributes.value: Illumina MiSeq 2x300 PE
attributes.tag: BioSampleModel
attributes.value: Metagenome or environmental