organism: insect metagenome
attributes.tag: collection_date
attributes.value: 2013-03
attributes.tag: env_biome
attributes.value: Deciduous woodland
attributes.tag: env_feature
attributes.value: Forest
attributes.tag: env_material
attributes.value: tick
attributes.tag: geo_loc_name
attributes.value: USA:Lake Dawson, Connecticut
attributes.tag: host
attributes.value: Ixodes scapularis
attributes.tag: lat_lon
attributes.value: 41.37 N 72.97 W
attributes.tag: host_life_stage
attributes.value: Adult
attributes.tag: host_sex
attributes.value: male
attributes.tag: host_taxid
attributes.value: 6945
attributes.tag: host_tissue_sampled
attributes.value: Whole tick
attributes.tag: samp_collect_device
attributes.value: flagging
attributes.tag: samp_size
attributes.value: 1 individual tick
attributes.tag: samp_store_temp
attributes.value: 4 C
attributes.tag: nucl_acid_ext
attributes.value: Sequentially washed in 10% bleach, 70% ethanol, and distilled water (3x), flash frozen in liquid nitrogen, ground with a pestle, and extracted using the Promega (Madison, WI, USA) Wizard SV 96 Genomic DNA Purification System.
attributes.tag: target_gene
attributes.value: 16S rDNA
attributes.tag: target_subfragment
attributes.value: V5-V3
attributes.tag: mid
attributes.value: AGGCGG
attributes.tag: pcr_primers
attributes.value: FWD:CCCGTCAATTCMTTTRAGT REV:CCTACGGGAGGCAGCAG
attributes.tag: seq_meth
attributes.value: pyrosequencing
attributes.tag: unique_seq_id
attributes.value: PTP776946:4:AGGCGG
attributes.tag: BioSampleModel
attributes.value: MIMARKS.survey
attributes.tag: BioSampleModel
attributes.value: MIGS/MIMS/MIMARKS.host-associated