organism: soil metagenome
attributes.tag: isolation_source
attributes.value: soil
attributes.tag: collection_date
attributes.value: 2015-06-20
attributes.tag: geo_loc_name
attributes.value: Canada: Wood Buffalo National Park
attributes.tag: lat_lon
attributes.value: 60.1361 N 112.6686 W
attributes.tag: samp_collect_device
attributes.value: Metal core, 5.5 cm i.d., variable depth up to 13.5 cm
attributes.tag: samp_mat_process
attributes.value: Preserved in Lifeguard soil preservation solution, as cold as possible in field, then frozen, and finally -80°C
attributes.tag: samp_size
attributes.value: 250 mg
attributes.tag: Barcodes
attributes.value: TGCTGGAAGGATAAGG
attributes.tag: Sequencing_Sample_Name
attributes.value: 15S-WB-10M-1
attributes.tag: Project_ID
attributes.value: WB15
attributes.tag: Sample_ID
attributes.value: 15S-WB-08O
attributes.tag: Site_ID
attributes.value: 15S-WB-08
attributes.tag: Soil_Extraction_Replicate
attributes.value: 1
attributes.tag: Org_or_Min
attributes.value: O
attributes.tag: Fwd_Primer_Barcode
attributes.value: TGCTGGAA
attributes.tag: Rev_Primer_Barcode
attributes.value: GGATAAGG
attributes.tag: Exch_Na_mg_kg
attributes.value: 0
attributes.tag: Exch_Mg_mg_kg
attributes.value: 0
attributes.tag: Exch_K_mg_kg
attributes.value: 0
attributes.tag: Exch_Ca_mg_kg
attributes.value: 0
attributes.tag: pH
attributes.value: 7.67
attributes.tag: EC_mS_cm
attributes.value: 0
attributes.tag: CEC_cmol_kg
attributes.value: 0
attributes.tag: Total_N_pct
attributes.value: 1.13
attributes.tag: Total_S_pct
attributes.value: 0
attributes.tag: Sand_pct
attributes.value: 0
attributes.tag: Clay_pct
attributes.value: 0
attributes.tag: Silt_pct
attributes.value: 0
attributes.tag: Ca_mg_kg
attributes.value: 137751
attributes.tag: K_mg_kg
attributes.value: 2506.8
attributes.tag: Mg_mg_kg
attributes.value: 5338.14
attributes.tag: Na_mg_kg
attributes.value: 150.788
attributes.tag: P_mg_kg
attributes.value: 4092.68
attributes.tag: S_mg_kg
attributes.value: 4634.06
attributes.tag: Al_mg_kg
attributes.value: 7208.53
attributes.tag: Fe_mg_kg
attributes.value: 7436.48
attributes.tag: Zn_mg_kg
attributes.value: 145.52
attributes.tag: Cu_mg_kg
attributes.value: 71.539
attributes.tag: Mn_mg_kg
attributes.value: 2509.69
attributes.tag: Mo_mg_kg
attributes.value: 58.837
attributes.tag: TIC_ash_pct
attributes.value: 5.23
attributes.tag: TOC_LOI_pct
attributes.value: 15.6728
attributes.tag: TOC_HCL_cruc_pct
attributes.value: 16.3
attributes.tag: TC_pct
attributes.value: 19.9
attributes.tag: Fire_ID
attributes.value: 2014SS-085
attributes.tag: Ecosite
attributes.value: d2.7
attributes.tag: Moisture
attributes.value: Mesic
attributes.tag: Moisture_Regime
attributes.value: 5
attributes.tag: Nutrient
attributes.value: Medium
attributes.tag: Community
attributes.value: Aw-Sw/ forb
attributes.tag: Forest
attributes.value: 1
attributes.tag: Plains
attributes.value: 1
attributes.tag: Interval
attributes.value: Long
attributes.tag: Veg_Comm
attributes.value: Mixedwood
attributes.tag: Land_Class_Unburned
attributes.value: Upland
attributes.tag: Land_Class
attributes.value: Upland
attributes.tag: Burned_Unburned
attributes.value: Burned
attributes.tag: Understory_CBI
attributes.value: 1.07
attributes.tag: Overstory_CBI
attributes.value: 1.41
attributes.tag: CBI
attributes.value: 1.2
attributes.tag: RBR
attributes.value: 227.7161052
attributes.tag: Pct_Exposed_Mineral
attributes.value: 72.5
attributes.tag: CFSI
attributes.value: 0.975
attributes.tag: Burn_Severity_Index
attributes.value: 4.35
attributes.tag: Mean_Duff_Depth_cm
attributes.value: 0
attributes.tag: O_Depth_cm
attributes.value: 0.5
attributes.tag: Dead_Trees
attributes.value: 25
attributes.tag: Live_Trees
attributes.value: 7
attributes.tag: nTrees
attributes.value: 32
attributes.tag: Severity_Class
attributes.value: Low
attributes.tag: temp
attributes.value: 27.25742778
attributes.tag: rh
attributes.value: 43.70734003
attributes.tag: ws
attributes.value: 9.937901397
attributes.tag: prec
attributes.value: 0.02189507
attributes.tag: ffmc
attributes.value: 89.69415315
attributes.tag: dmc
attributes.value: 52.56761833
attributes.tag: dc
attributes.value: 419.6075889
attributes.tag: isi
attributes.value: 6.770537731
attributes.tag: bui
attributes.value: 80.06063661
attributes.tag: fwi
attributes.value: 21.48707302
attributes.tag: Correct_ID
attributes.value: 15S-WB-08O-1
attributes.tag: Number_Fires_Recorded
attributes.value: 1
attributes.tag: TSLF
attributes.value: 80
attributes.tag: TSO
attributes.value: 80
attributes.tag: Fires_PerYear
attributes.value: 0.0125
attributes.tag: Fires_PerDecade_Recording
attributes.value: 0.125
attributes.tag: Longest_Period_Btw_Fires
attributes.value: 80
attributes.tag: Shortest_Period_Btw_Fires
attributes.value: 80
attributes.tag: Mean_Period_Btw_Fires
attributes.value: 80
attributes.tag: Reburn_Severity_Index
attributes.value: 0.999923319
attributes.tag: Overstory_Dens_ha
attributes.value: 847.976096
attributes.tag: Understory_Dens_ha
attributes.value: 0
attributes.tag: Abs_Density_Ha_UnderOver
attributes.value: 847.976096
attributes.tag: PINUBAN_Stems_Ha
attributes.value: 0
attributes.tag: PICEMAR_Stems_Ha
attributes.value: 0
attributes.tag: PICEGLA_Stems_Ha
attributes.value: 503.485807
attributes.tag: LARILAR_Stems_Ha
attributes.value: 0
attributes.tag: POPUTRE_Stems_Ha
attributes.value: 344.490289
attributes.tag: POPUBAL_Stems_Ha
attributes.value: 0
attributes.tag: BETUPAP_Stems_Ha
attributes.value: 0
attributes.tag: PINUBAN_BA_per_Ha
attributes.value: 0
attributes.tag: PICEMAR_BA_per_Ha
attributes.value: 0
attributes.tag: PICEGLA_BA_per_Ha
attributes.value: 15.14550108
attributes.tag: LARILAR_BA_per_Ha
attributes.value: 0
attributes.tag: POPUTRE_BA_per_Ha
attributes.value: 5.322454831
attributes.tag: POPUBAL_BA_per_Ha
attributes.value: 0
attributes.tag: BETUPAP_BA_per_Ha
attributes.value: 0
attributes.tag: BA_ha
attributes.value: 20.46795591
attributes.tag: Pct_Conifer_Stems_lar
attributes.value: 59.375
attributes.tag: Pct_Conifer_BA_lar
attributes.value: 73.99615841
attributes.tag: Median_CBH
attributes.value: 1.65
attributes.tag: Flammable_T_ha
attributes.value: 14.92115658
attributes.tag: Conifer_CBD
attributes.value: 0.081468552
attributes.tag: Surface_Severity_Class
attributes.value: High
attributes.tag: Soil_Class
attributes.value: Orthic Gleysol
attributes.tag: env_biome
attributes.value: boreal
attributes.tag: env_feature
attributes.value: Burned Upland Mixedwood
attributes.tag: env_material
attributes.value: soil
attributes.tag: env_package
attributes.value: mimarks
attributes.tag: nucl_acid_ext
attributes.value: Qiagen / MoBio PowerLyzer PowerSoil DNA extraction kit
attributes.tag: target_gene
attributes.value: rRNA gene operon
attributes.tag: target_subfragment
attributes.value: ITS2
attributes.tag: pcr_primers
attributes.value: FWD: AATGATACGGCGACCACCGAGATCTACACNNNNNNNNTATGGTAATTAAAGCCTCCGCTTATTGATATGCTTAART REV: CAAGCAGAAGACGGCATACGAGATNNNNNNNNAGTCAGTCAGGGAACTTTYRRCAAYGGATCWCT
attributes.tag: pcr_cond
attributes.value: initial denaturation:98_2; denaturation:98_0.167; annealing:57_0.25; elongation:72_0.167; final elongation:72_2; total cycles: 30
attributes.tag: seq_meth
attributes.value: Illumina MiSeq 2x300 PE
attributes.tag: BioSampleModel
attributes.value: Metagenome or environmental