organism: Prochilodus magdalenae
attributes.tag: strain
attributes.value: P. magdalenae
attributes.tag: cultivar
attributes.value: Institute of Fish Culture Research-CINPIC
attributes.tag: age
attributes.value: 2 years
attributes.tag: dev_stage
attributes.value: adult
attributes.tag: sex
attributes.value: female
attributes.tag: tissue
attributes.value: Gonad
attributes.tag: biomaterial_provider
attributes.value: Institute of Fish Culture Research - CINPIC
attributes.tag: birth_date
attributes.value: Monteria: Cordoba
attributes.tag: birth_location
attributes.value: Institute of Fish Culture Research - CINPIC
attributes.tag: collected_by
attributes.value: University of Cartagena
attributes.tag: collection_date
attributes.value: 2016-11-10
attributes.tag: death_date
attributes.value: 10-Nov-2016
attributes.tag: disease
attributes.value: None
attributes.tag: disease_stage
attributes.value: None
attributes.tag: geo_loc_name
attributes.value: Colombia: University of Cordoba. Monteria-Cordoba
attributes.tag: health_state
attributes.value: Fish in a healthy state
attributes.tag: isolation_source
attributes.value: This study was conducted at the Fish Research Center of the University of Cordoba (CINPIC; Monteria, Colombia), two-year-old female bocachico were kept in earthen ponds (density: 0.5 kg / m2).
attributes.tag: lat_lon
attributes.value: 8.79 N 75.86 W
attributes.tag: sample_type
attributes.value: tissue sample
attributes.tag: specimen_voucher
attributes.value: Electronic images
attributes.tag: store_cond
attributes.value: The fish was anesthetized by loss of swimming line by gradually decreasing the water temperature with ice cubes, then euthanized and dissected by an incision in the ventral midline of the fish, from the interopercular space to the anus obtaining the gonadal tissue. This gonad sample was immediately subjected to RNA extraction, with the TRIzol reagent -Invitrogen- according to the manufacturers specifications. The sample was transported in dry ice at -80grados C to the National Center for Genomic Sequencing-CNSG of the University of Antioquia for the analysis of the gonadal transcriptome of P. magdalenae fish through the second generation Sequencing Service. Sequencing service requested by the University of Cartagena, Environmental and Computational Chemistry group.
attributes.tag: BioSampleModel
attributes.value: Model organism or animal