organism: soil metagenome
attributes.tag: isolation_source
attributes.value: soil
attributes.tag: collection_date
attributes.value: 2015-07-12
attributes.tag: geo_loc_name
attributes.value: Canada: Northwest Territories
attributes.tag: lat_lon
attributes.value: 61.5284 N 117.2238 W
attributes.tag: samp_collect_device
attributes.value: Metal core, 5.5 cm i.d., variable depth up to 13.5 cm
attributes.tag: samp_mat_process
attributes.value: Preserved in Lifeguard soil preservation solution, as cold as possible in field, then frozen, and finally -80°C
attributes.tag: samp_size
attributes.value: 250 mg
attributes.tag: Barcodes
attributes.value: ACTGCGCGTTAGGCTC
attributes.tag: Sequencing_Sample_Name
attributes.value: Blank-22
attributes.tag: Project_ID
attributes.value: WB15
attributes.tag: Sample_ID
attributes.value: 15S-NT-U11O
attributes.tag: Site_ID
attributes.value: 15S-NT-U11
attributes.tag: Soil_Extraction_Replicate
attributes.value: 2
attributes.tag: Org_or_Min
attributes.value: O
attributes.tag: Fwd_Primer_Barcode
attributes.value: ACTGCGCG
attributes.tag: Rev_Primer_Barcode
attributes.value: TTAGGCTC
attributes.tag: Exch_Na_mg_kg
attributes.value: 0
attributes.tag: Exch_Mg_mg_kg
attributes.value: 0
attributes.tag: Exch_K_mg_kg
attributes.value: 0
attributes.tag: Exch_Ca_mg_kg
attributes.value: 0
attributes.tag: pH
attributes.value: 6.73
attributes.tag: EC_mS_cm
attributes.value: 2.127
attributes.tag: CEC_cmol_kg
attributes.value: 0
attributes.tag: Total_N_pct
attributes.value: 2.24
attributes.tag: Total_S_pct
attributes.value: 0
attributes.tag: Sand_pct
attributes.value: 0
attributes.tag: Clay_pct
attributes.value: 0
attributes.tag: Silt_pct
attributes.value: 0
attributes.tag: Ca_mg_kg
attributes.value: 36433.7
attributes.tag: K_mg_kg
attributes.value: 555.498
attributes.tag: Mg_mg_kg
attributes.value: 6155.77
attributes.tag: Na_mg_kg
attributes.value: 396.053
attributes.tag: P_mg_kg
attributes.value: 744.881
attributes.tag: S_mg_kg
attributes.value: 12042.3
attributes.tag: Al_mg_kg
attributes.value: 505.383
attributes.tag: Fe_mg_kg
attributes.value: 3399.51
attributes.tag: Zn_mg_kg
attributes.value: 22.448
attributes.tag: Cu_mg_kg
attributes.value: 144.483
attributes.tag: Mn_mg_kg
attributes.value: 506.377
attributes.tag: Mo_mg_kg
attributes.value: 45.013
attributes.tag: TIC_ash_pct
attributes.value: 12
attributes.tag: TOC_LOI_pct
attributes.value: 48.4896
attributes.tag: TOC_HCL_cruc_pct
attributes.value: 45.9
attributes.tag: TC_pct
attributes.value: 44.2
attributes.tag: Fire_ID
attributes.value: 2014ZF-020
attributes.tag: Ecosite
attributes.value: j2.1
attributes.tag: Moisture
attributes.value: Subhydric
attributes.tag: Moisture_Regime
attributes.value: 8
attributes.tag: Nutrient
attributes.value: Medium
attributes.tag: Community
attributes.value: Willow/golden moss
attributes.tag: Forest
attributes.value: 0
attributes.tag: Plains
attributes.value: 1
attributes.tag: Interval
attributes.value: 0
attributes.tag: Veg_Comm
attributes.value: Open Wetland
attributes.tag: Land_Class_Unburned
attributes.value: Unburned
attributes.tag: Land_Class
attributes.value: Wetland
attributes.tag: Burned_Unburned
attributes.value: Unburned
attributes.tag: Understory_CBI
attributes.value: 0
attributes.tag: Overstory_CBI
attributes.value: 0
attributes.tag: CBI
attributes.value: 0
attributes.tag: RBR
attributes.value: 13.4960772
attributes.tag: Pct_Exposed_Mineral
attributes.value: 0
attributes.tag: CFSI
attributes.value: 0
attributes.tag: Burn_Severity_Index
attributes.value: 1
attributes.tag: Mean_Duff_Depth_cm
attributes.value: 0
attributes.tag: O_Depth_cm
attributes.value: 0
attributes.tag: Dead_Trees
attributes.value: 0
attributes.tag: Live_Trees
attributes.value: 32
attributes.tag: nTrees
attributes.value: 32
attributes.tag: Severity_Class
attributes.value: Unburned
attributes.tag: temp
attributes.value: 0
attributes.tag: rh
attributes.value: 0
attributes.tag: ws
attributes.value: 0
attributes.tag: prec
attributes.value: 0
attributes.tag: ffmc
attributes.value: 0
attributes.tag: dmc
attributes.value: 0
attributes.tag: dc
attributes.value: 0
attributes.tag: isi
attributes.value: 0
attributes.tag: bui
attributes.value: 0
attributes.tag: fwi
attributes.value: 0
attributes.tag: Correct_ID
attributes.value: 15S-NT-U11O-2
attributes.tag: Soil_Class
attributes.value: Typic Mesisol
attributes.tag: env_biome
attributes.value: boreal
attributes.tag: env_feature
attributes.value: Unburned Wetland Open Wetland
attributes.tag: env_material
attributes.value: soil
attributes.tag: env_package
attributes.value: mimarks
attributes.tag: nucl_acid_ext
attributes.value: Qiagen / MoBio PowerLyzer PowerSoil DNA extraction kit
attributes.tag: target_gene
attributes.value: 16S rRNA
attributes.tag: target_subfragment
attributes.value: V4
attributes.tag: pcr_primers
attributes.value: FWD: AATGATACGGCGACCACCGAGATCTACACNNNNNNNNTATGGTAATTGTGTGYCAGCMGCCGCGGTAA REV: CAAGCAGAAGACGGCATACGAGATNNNNNNNNAGTCAGCCAGCCGGACTACNVGGGTWTCTAAT
attributes.tag: pcr_cond
attributes.value: initial denaturation:98_2; denaturation:98_0.167; annealing:58_0.25; elongation:72_0.167; final elongation:72_2; total cycles: 30
attributes.tag: seq_meth
attributes.value: Illumina MiSeq 2x250 PE
attributes.tag: BioSampleModel
attributes.value: Metagenome or environmental