organism: Mus musculus
attributes.tag: sample_name
attributes.value: DRS012047
attributes.tag: 14.TSA_2h_02
attributes.value: Adult mouse treated with TSA. Two hours after TSA injection, mouse was sacrificed and cerebral cortex was isolated. Pyramidal cells were sorted by FACS and total RNAs were extracted to prepare nanoCAGE library. barcode:5'SOLX_96nCAGE_50 CTGCGT
attributes.tag: 15.TSA_2h_03
attributes.value: Adult mouse treated with TSA. Two hours after TSA injection, mouse was sacrificed and cerebral cortex was isolated. Pyramidal cells were sorted by FACS and total RNAs were extracted to prepare nanoCAGE library. barcode:5'SOLX_96nCAGE_55 GAGACG
attributes.tag: 16.VEH/tsa_2h_01
attributes.value: Adult mouse treated with only vehicle used for TSA (Control mouse for TSA). Two hours after vehicle injection, mouse was sacrificed and cerebral cortex was isolated. Pyramidal cells were sorted by FACS and total RNAs were extracted to prepare nanoCAGE library. barcode:5'SOLX_96nCAGE_63 GAGTGA
attributes.tag: 17.VEH/tsa_2h_02
attributes.value: Adult mouse treated with only vehicle used for TSA (Control mouse for TSA). Two hours after vehicle injection, mouse was sacrificed and cerebral cortex was isolated. Pyramidal cells were sorted by FACS and total RNAs were extracted to prepare nanoCAGE library. barcode:5'SOLX_96nCAGE_66 GCTATA
attributes.tag: 18.VEH/tsa_2h_03
attributes.value: Adult mouse treated with only vehicle used for TSA (Control mouse for TSA). Two hours after vehicle injection, mouse was sacrificed and cerebral cortex was isolated. Pyramidal cells were sorted by FACS and total RNAs were extracted to prepare nanoCAGE library. barcode:5'SOLX_96nCAGE_68 GCTCGT
attributes.tag: 19.TSA_2d_01
attributes.value: Adult mouse treated with TSA. Two days after continuous TSA injections, mouse was sacrificed and cerebral cortex was isolated. Pyramidal cells were sorted by FACS and total RNAs were extracted to prepare nanoCAGE library. barcode:5'SOLX_96nCAGE_74 GTACGT
attributes.tag: 2.VPA_2h_02
attributes.value: Adult mouse treated with VPA. Two hours after VPA injection, mouse was sacrificed and cerebral cortex was isolated. Pyramidal cells were sorted by FACS and total RNAs were extracted to prepare nanoCAGE library. barcode:5'SOLX_96nCAGE_04 ACAGAT
attributes.tag: 20.TSA_2d_02
attributes.value: Adult mouse treated with TSA. Two days after continuous TSA injections, mouse was sacrificed and cerebral cortex was isolated. Pyramidal cells were sorted by FACS and total RNAs were extracted to prepare nanoCAGE library. barcode:5'SOLX_96nCAGE_78 GTAGTG
attributes.tag: 21.TSA_2d_03
attributes.value: Adult mouse treated with TSA. Two days after continuous TSA injections, mouse was sacrificed and cerebral cortex was isolated. Pyramidal cells were sorted by FACS and total RNAs were extracted to prepare nanoCAGE library. barcode:5'SOLX_96nCAGE_79 GTATAC
attributes.tag: 22.VEH/tsa_2d_01
attributes.value: Adult mouse treated with only vehicle used for TSA (Control mouse for TSA). Two days after continuous vehicle injections, mouse was sacrificed and cerebral cortex was isolated. Pyramidal cells were sorted by FACS and total RNAs were extracted to prepare nanoCAGE library. barcode:5'SOLX_96nCAGE_86 TATCGT
attributes.tag: 1.VPA_2h_01
attributes.value: Adult mouse treated with VPA. Two hours after VPA injection, mouse was sacrificed and cerebral cortex was isolated. Pyramidal cells were sorted by FACS and total RNAs were extracted to prepare nanoCAGE library. barcode:5'SOLX_96nCAGE_02 ACACGT
attributes.tag: 24.VEH/tsa_2d_03
attributes.value: Adult mouse treated with only vehicle used for TSA (Control mouse for TSA). Two days after continuous vehicle injections, mouse was sacrificed and cerebral cortex was isolated. Pyramidal cells were sorted by FACS and total RNAs were extracted to prepare nanoCAGE library. barcode:5'SOLX_96nCAGE_91 TCGACG
attributes.tag: 3.VPA_2h_03
attributes.value: Adult mouse treated with VPA. Two hours after VPA injection, mouse was sacrificed and cerebral cortex was isolated. Pyramidal cells were sorted by FACS and total RNAs were extracted to prepare nanoCAGE library. barcode:5'SOLX_96nCAGE_06 ACAGTG
attributes.tag: 4.VEH/vpa_2h_01
attributes.value: Adult mouse treated with only vehicle used for VPA (Control mouse for VPA). Two hours after vehicle injection, mouse was sacrificed and cerebral cortex was isolated. Pyramidal cells were sorted by FACS and total RNAs were extracted to prepare nanoCAGE library. barcode:5'SOLX_96nCAGE_07 ACATAC
attributes.tag: 5.VEH/vpa_2h_02
attributes.value: Adult mouse treated with only vehicle used for VPA (Control mouse for VPA). Two hours after vehicle injection, mouse was sacrificed and cerebral cortex was isolated. Pyramidal cells were sorted by FACS and total RNAs were extracted to prepare nanoCAGE library. barcode:5'SOLX_96nCAGE_14 AGTCGT
attributes.tag: 6.VEH/vpa_2h_03
attributes.value: Adult mouse treated with only vehicle used for VPA (Control mouse for VPA). Two hours after vehicle injection, mouse was sacrificed and cerebral cortex was isolated. Pyramidal cells were sorted by FACS and total RNAs were extracted to prepare nanoCAGE library. barcode:5'SOLX_96nCAGE_16 AGTGAT
attributes.tag: 7.VPA_2d_01
attributes.value: Adult mouse treated with VPA. Two days after continuous VPA injections, mouse was sacrificed and cerebral cortex was isolated. Pyramidal cells were sorted by FACS and total RNAs were extracted to prepare nanoCAGE library. barcode:5'SOLX_96nCAGE_18 AGTGTG
attributes.tag: 8.VPA_2d_02
attributes.value: Adult mouse treated with VPA. Two days after continuous VPA injections, mouse was sacrificed and cerebral cortex was isolated. Pyramidal cells were sorted by FACS and total RNAs were extracted to prepare nanoCAGE library. barcode:5'SOLX_96nCAGE_22 ATCGAT
attributes.tag: 9.VPA_2d_03
attributes.value: Adult mouse treated with VPA. Two days after continuous VPA injections, mouse was sacrificed and cerebral cortex was isolated. Pyramidal cells were sorted by FACS and total RNAs were extracted to prepare nanoCAGE library. barcode:5'SOLX_96nCAGE_27 ATCTGA
attributes.tag: sample comment
attributes.value: 24 nanoCAGE/CAGEscan libraries were prepared from total RNAs of Fluorescence-activated cell sorting (FACS) sorted pyramidal neurons ranging from 30-100 ng, where four treatments including two HDACi (TSA and VPA) with their vehicle controls, two time points after each treatment (2h and 2d) were compared. Three biological replicates for each combination of treatment and time point were prepared. In order to sequence multiplex libraries at a time, a six nucleotide barcode system was introduced. Briefly, the barcodes were introduced inside the template-switching primer and incorporated at the 5′-end of the nanoCAGE libraries through reverse transcription. After cDNA synthesis, 24 libraries were pooled into single library and adapted for sequencing with an Illumina Genome analyzer IIx.
attributes.tag: 23.VEH/tsa_2d_02
attributes.value: Adult mouse treated with only vehicle used for TSA (Control mouse for TSA). Two days after continuous vehicle injections, mouse was sacrificed and cerebral cortex was isolated. Pyramidal cells were sorted by FACS and total RNAs were extracted to prepare nanoCAGE library. barcode:5'SOLX_96nCAGE_90 TATGTG
attributes.tag: 10.VEH/vpa_2d_01
attributes.value: Adult mouse treated with only vehicle used for VPA (Control mouse for VPA). Two days after continuous vehicle injections, mouse was sacrificed and cerebral cortex was isolated. Pyramidal cells were sorted by FACS and total RNAs were extracted to prepare nanoCAGE library. barcode:5'SOLX_96nCAGE_30 CACATA
attributes.tag: 11.VEH/vpa_2d_02
attributes.value: Adult mouse treated with only vehicle used for VPA (Control mouse for VPA). Two days after continuous vehicle injections, mouse was sacrificed and cerebral cortex was isolated. Pyramidal cells were sorted by FACS and total RNAs were extracted to prepare nanoCAGE library. barcode:5'SOLX_96nCAGE_31 CACGAT
attributes.tag: 12.VEH/vpa_2d_03
attributes.value: Adult mouse treated with only vehicle used for VPA (Control mouse for VPA). Two days after continuous vehicle injections, mouse was sacrificed and cerebral cortex was isolated. Pyramidal cells were sorted by FACS and total RNAs were extracted to prepare nanoCAGE library. barcode:5'SOLX_96nCAGE_38 CGACGT
attributes.tag: 13.TSA_2h_01
attributes.value: Adult mouse treated with TSA. Two hours after TSA injection, mouse was sacrificed and cerebral cortex was isolated. Pyramidal cells were sorted by FACS and total RNAs were extracted to prepare nanoCAGE library. barcode:5'SOLX_96nCAGE_43 CGATAC