organism: Magallana gigas
attributes.tag: ENA first public
attributes.value: 2014-07-22
attributes.tag: ENA last update
attributes.value: 2016-10-21
attributes.tag: External Id
attributes.value: SAMEA2670340
attributes.tag: INSDC center alias
attributes.value: ALFRED WEGENER INSTITUTE FOR POLAR AND MARINE RESE
attributes.tag: INSDC center name
attributes.value: ALFRED WEGENER INSTITUTE FOR POLAR AND MARINE RESE
attributes.tag: INSDC first public
attributes.value: 2014-07-22T17:15:39Z
attributes.tag: INSDC last update
attributes.value: 2016-10-21T09:38:56Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: 1911_15
attributes.tag: adapters
attributes.value: f: adapter A + key (4bp): CGTATCGCCTCCCTCGCGCCATCAG; r: adapter B + key (4bp): CTATGCGCCTTGCCAGCCCGCTCAG
attributes.tag: amount or size of sample collected
attributes.value: 0.0002
attributes.tag: body habitat
attributes.value: hemolymph
attributes.tag: body product
attributes.value: hemolymph
attributes.tag: body site
attributes.value: adductor muscle
attributes.tag: collection date
attributes.value: 2010-11-19
attributes.tag: common name
attributes.value: Pacific oyster
attributes.tag: detailed conditions
attributes.value: 3 days after transfer from flowthrough to stationary seawater aquarium (both 8°C) and injection with soya-peptone medium
attributes.tag: disease status
attributes.value: healthy
attributes.tag: environment (biome)
attributes.value: aquatic, marine
attributes.tag: environment (feature)
attributes.value: organic
attributes.tag: environment (material)
attributes.value: organic material, bodily fluid
attributes.tag: geographic location (countryand/orsea,region)
attributes.value: Germany
attributes.tag: geographic location (latitude)
attributes.value: 55.0288889
attributes.tag: geographic location (longitude)
attributes.value: 8.4341667
attributes.tag: host body temperature
attributes.value: 8
attributes.tag: host common name
attributes.value: Pacific oyster
attributes.tag: host subject id
attributes.value: 15
attributes.tag: host taxid
attributes.value: 29159
attributes.tag: host-associated environmental package
attributes.value: host-associated
attributes.tag: investigation type
attributes.value: mimarks-survey
attributes.tag: life stage
attributes.value: adult
attributes.tag: location details
attributes.value: Schleswig-Holstein, Wadden Sea (North Sea), island of Sylt, Kongishafen
attributes.tag: multiplex identifiers
attributes.value: f: CGTCTAGTAC; r: AGACGCACTC or f: CGTCTAGTAC; r: ATATCGCGAG
attributes.tag: pcr conditions
attributes.value: initial denaturation:94degC_1min; denaturation:94degC_40s;annealing:55degC_30s; elongation:72degC_30s; final elongation:72degC_2min; 35 cycles
attributes.tag: pcr primers
attributes.value: f: linker (3bp) + 16S_338r: CATGCTGCCTCCCGTAGGAGT; r: linker (2bp) + 16S_27F: TCAGAGTTTGATCMTGGCTCAG
attributes.tag: perturbation
attributes.value: temperature acclimated, mock-infected
attributes.tag: project name
attributes.value: PRJEB5702
attributes.tag: sample collection device or method
attributes.value: syringe
attributes.tag: sample name
attributes.value: 1911_15
attributes.tag: sequencing method
attributes.value: pyrosequencing
attributes.tag: substrate
attributes.value: seawater (local, supplied through cca 200 m long pipe, 30 um filtered and protein skimmed)
attributes.tag: target gene
attributes.value: 16S rRNA
attributes.tag: target subfragment
attributes.value: V1-V2
attributes.tag: temperature
attributes.value: 8