organism: marine metagenome
attributes.tag: Chlorophyll Sensor
attributes.value: 0.00765
attributes.tag: Citation
attributes.value: Tara Oceans Consortium, Coordinators & Tara Oceans Expedition, Participants. Registry of all samples from the Tara Oceans Expedition (2009-2013). PANGAEA. doi:10.1594/PANGAEA.842197 (2015) (https://dx.doi.org/10.1594/PANGAEA.842197)
attributes.tag: ENA first public
attributes.value: 2016-10-28
attributes.tag: ENA last update
attributes.value: 2016-08-25
attributes.tag: Event Date/Time End
attributes.value: 2013-07-08T07:55:00
attributes.tag: External Id
attributes.value: SAMEA4397249
attributes.tag: INSDC center alias
attributes.value: GSC
attributes.tag: INSDC center name
attributes.value: Genoscope
attributes.tag: INSDC first public
attributes.value: 2016-10-28T17:01:46Z
attributes.tag: INSDC last update
attributes.value: 2016-08-25T11:55:00Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Latitude End
attributes.value: 78.9222
attributes.tag: Longitude End
attributes.value: 79.2246
attributes.tag: Salinity Sensor
attributes.value: 33.595737
attributes.tag: Submitter Id
attributes.value: TARA_B110000083
attributes.tag: depth
attributes.value: 5
attributes.tag: environment (biome)
attributes.value: Polar Biome
attributes.tag: environment (feature)
attributes.value: [SRF] surface water layer (ENVO:00010504)
attributes.tag: environment (feature) further details
attributes.value: discrete depth within the epipelagic zone (ENVO:00000209) at the top of the photic zone (ENVO:00000209), <50 m from a strong peak in chlorophyll fluorescence
attributes.tag: environment (material)
attributes.value: water (ENVO:00002006), including particulate matter (ENVO:01000060) such as planktonic material (ENVO:01000063)
attributes.tag: environmental package
attributes.value: water
attributes.tag: event date/time start
attributes.value: 2013-07-08T04:12:00
attributes.tag: event label
attributes.value: TARA_20130708T0412Z_173_EVENT_PUMP
attributes.tag: further details
attributes.value: http://store.pangaea.de/Projects/TARA-OCEANS/Logsheets_Wetlab/TARA_20130708Z_173_WETLAB_BACT.pdf
attributes.tag: last update date
attributes.value: 2016-04-23Z
attributes.tag: latitude start
attributes.value: 78.9564
attributes.tag: longitude start
attributes.value: 79.4201
attributes.tag: marine region
attributes.value: [AO] Arctic Ocean (MRGID:1906)
attributes.tag: nitrate sensor
attributes.value: 2.40637
attributes.tag: oxygen sensor
attributes.value: 383.163125
attributes.tag: project name
attributes.value: Tara Oceans Expedition (2009-2013)
attributes.tag: protocol description
attributes.value: In the size fraction W0.22-3 micrometres, the sample material was pre-filtered on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane (ref:TSTP14250), and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 10 Litres was filtered and the 0.22-micrometre-pore-size filter was packaged in a sterile Falcon tube (50 mL), treated with the addition of 10 mL of RNA-Later, labelled with a barcode identification sticker and stored on board in a freezer at -20 degree Celsius. Note 1: Latex or nitril gloves were used for this protocol. Note 2: All containers, filter holders and tubing were washed with 0.1% bleach, rinsed twice with fresh water and rinsed again twice with the pre-filtered sample material. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump (Masterflex, EW-77410-10) was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. Note 5: The entire process did not exceed 15 minutes. A total of 2 replicates were prepared and sent for later analysis to the Institute of Marine Science (CSIC), Barcelona, Spain.
attributes.tag: protocol label
attributes.value: SEQ-(10L-or-15min)_W0.22-3
attributes.tag: sample collection device
attributes.value: high volume peristaltic pump [HVP-PUMP]
attributes.tag: sample material label
attributes.value: TARA_173_SRF_0.22-3
attributes.tag: sample name
attributes.value: TARA_B110000083
attributes.tag: sample status
attributes.value: This version can be used to provide data discovery services
attributes.tag: sampling campaign
attributes.value: TARA_20130629Z
attributes.tag: sampling platform
attributes.value: SV Tara
attributes.tag: sampling station
attributes.value: TARA_173
attributes.tag: size fraction lower threshold
attributes.value: 0.22
attributes.tag: size fraction upper threshold
attributes.value: 3
attributes.tag: temperature
attributes.value: -0.083125