organism: Saccharomyces cerevisiae
attributes.tag: strain
attributes.value: ROY5634 (library)
attributes.tag: isolation_source
attributes.value: ROY5634
attributes.tag: collection_date
attributes.value: not collected
attributes.tag: geo_loc_name
attributes.value: not applicable
attributes.tag: sample_type
attributes.value: FACS
attributes.tag: genotype
attributes.value: MATa ADE2 lys2D leu2-3,112 his3-11 ura3-1 trp1-1 can1-100 rpl18b::BFP2-2
attributes.tag: dev_stage
attributes.value: OD600 described in growth protocol
attributes.tag: tissue
attributes.value: liquid culture
attributes.tag: growth_protocol
attributes.value: 5ml of yeast cells containing the library were used to inoculate 50ml YMD medium containing adenine, leucine, lysine, histidine and tryptophan (lacking uracil) and cells were grown at room temperature overnight (approximately 3 doublings). This culture was then used to inoculate 250ml YMD-uracil and grown at 30C for 3h. Cells were pelleted and resuspended into 250ul 1xPBS and 1%BSA at a concentration of 1 OD/ml, filtered through a Nitex mesh and sorted.
attributes.tag: treatment
attributes.value: sorted
attributes.tag: BioSampleModel
attributes.value: Microbe, viral or environmental