organism: metagenome
attributes.tag: ENA first public
attributes.value: 2016-10-30
attributes.tag: ENA last update
attributes.value: 2016-10-21
attributes.tag: External Id
attributes.value: SAMEA3598032
attributes.tag: INSDC center alias
attributes.value: Thuenen Institute of Biodiversity
attributes.tag: INSDC center name
attributes.value: Thuenen Institute of Biodiversity
attributes.tag: INSDC first public
attributes.value: 2016-10-30T17:09:20Z
attributes.tag: INSDC last update
attributes.value: 2016-10-21T09:34:05Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: S39
attributes.tag: collection date
attributes.value: 2011-08
attributes.tag: current land use
attributes.value: Arable field
attributes.tag: current vegetation
attributes.value: grass-clover mixture: Medicago sativa, Trifolium hybridum, Lotus corniculatus, Lolium perenne, Festuca pratense, Phleum pratense
attributes.tag: depth
attributes.value: 0.18
attributes.tag: environment (biome)
attributes.value: Temporate broadleaf forest biome
attributes.tag: environment (feature)
attributes.value: Arable filed
attributes.tag: environment (material)
attributes.value: Arable soil
attributes.tag: geographic location (country and/or sea)
attributes.value: Denmark
attributes.tag: geographic location (elevation)
attributes.value: 63
attributes.tag: geographic location (latitude)
attributes.value: +55.47
attributes.tag: geographic location (longitude)
attributes.value: +07.12
attributes.tag: history/agrochemical additions
attributes.value: fertilisation 18 months before sampling; NPK: 100 kg total-N ha-1, 19 kg P ha-1 and 87 kg K ha-1; AM: 37.5 t wet weight cattle slurry ha-1
attributes.tag: history/crop rotation
attributes.value: Winter wheat, beet roots, barley, grass-clover mixture
attributes.tag: horizon
attributes.value: A horizon
attributes.tag: investigation type
attributes.value: metagenome
attributes.tag: mean annual and seasonal precipitation
attributes.value: 862
attributes.tag: mean annual and seasonal temperature
attributes.value: 7.7
attributes.tag: nucleic acid extraction
attributes.value: FastDNA™ SPIN Kit for Soil (MP Biomedicals, Santa Ana, CA)
attributes.tag: ph
attributes.value: 6.3
attributes.tag: ph method
attributes.value: CaCl2
attributes.tag: project name
attributes.value: SPP 1315 Biogeochemical Interfaces in Soil
attributes.tag: sample material processing
attributes.value: mild sonication, wet sieving, centrifugation (see http://dx.doi.org/10.1016/j.soilbio.2015.08.018)
attributes.tag: sample name
attributes.value: S39
attributes.tag: sequencing method
attributes.value: Illumina MiSeq, dual-index paried-end multiplexing
attributes.tag: soil environmental package
attributes.value: soil
attributes.tag: soil taxonomic/fao classification
attributes.value: Typic Hapludalf
attributes.tag: storage conditions (fresh/frozen/other)
attributes.value: 50-55% water holding capacity, 4°C, darkness
attributes.tag: target gene
attributes.value: 16S rRNA gene
attributes.tag: target subfragment
attributes.value: V4
attributes.tag: texture
attributes.value: 64/26/10