organism: metagenome
attributes.tag: isolation_source
attributes.value: Adlie Penguin cloaca
attributes.tag: collection_date
attributes.value: 2015-12-27
attributes.tag: geo_loc_name
attributes.value: Antarctica: Kinnes Cove, Madder Cliff Joinville Island, Northeast Antarctic Peninsula
attributes.tag: lat_lon
attributes.value: 63.3 S 56.483 W
attributes.tag: samp_collect_device
attributes.value: Regular flocked swabs (Eswab, Copan, Murrieta, CA, USA) were used for cloacal swabs as part of gastrointestinal microbial and viral analysis from penguins. The swabs were stored in 1 ml of liquid Amies medium.
attributes.tag: samp_mat_process
attributes.value: Viral DNA was extracted from 200 l of the Amies medium suspension after vortexing with the High Pure Viral Nucleic Acid Kit (Roche Diagnostics, Indianapolis, IN, USA). The resulting viral DNA extraction was subjected to rolling circle amplification (RCA) using the TempliPhi 100 Amplification Kit (GE Healthcare, Chicago, IL, USA). An aliquot of the resulting RCA-amplified DNA (5 l) was pooled and used to generate Illumina sequencing libraries using the Nextera DNA Flex Library Prep Kit (Illumina Inc, San Diego, CA, USA). The libraries were sequenced on an Illumina 4000 sequencer (2 100 bp library)
attributes.tag: samp_size
attributes.value: 14: (adults=7; chicks=7)
attributes.tag: BioSampleModel
attributes.value: Metagenome or environmental