organism: Mus musculus
attributes.tag: strain
attributes.value: C57BL/6
attributes.tag: age
attributes.value: 8-10 weeks
attributes.tag: sex
attributes.value: male
attributes.tag: tissue
attributes.value: Lymph node, CD4+
attributes.tag: genotype
attributes.value: Bcl11b[fl/fl]-CreERT2
attributes.tag: growth_protocol
attributes.value: Th2 cell culture: Na•ve CD4 T cells (CD4+CD44lowCD62LhighCD25-) were purified from lymph node. Cells cultured in RPMI medium 1640 (Invitrogen) supplemented with 10% FBS (Hyclone), 200 mM Glutamine, 100 mM Sodium pyruvate (Gibco), 50__M __-mercaptoethanol (Sigma), 100 U/ml penicillin and 100 _g/ml streptomycin at 37_¡C in a humidified atmosphere of 5% CO2. a-IFN-g 10_g/ml, a-IL-12 10_g/ml, IL-4 5000U/ml. Na•ve CD4 T cells from Bcl11bfl/fl-CreERT2 mice cultured under Th2 conditions with 4-OHT for 4 days, 1 day resting, then an additional 4 days of Th2 conditions with 4-OHT.
attributes.tag: sample_type
attributes.value: cell culture
attributes.tag: BioSampleModel
attributes.value: Model organism or animal