organism: food fermentation metagenome
attributes.tag: host
attributes.value: Bos taurus
attributes.tag: isolation_source
attributes.value: Fermented milk products
attributes.tag: collection_date
attributes.value: 2016-04-02
attributes.tag: geo_loc_name
attributes.value: India: Arunachal Pradesh
attributes.tag: lat_lon
attributes.value: 27.3428 N 92.3024 E
attributes.tag: collection_time
attributes.value: 7:15:15
attributes.tag: replicated_pcr_that_are_pooled
attributes.value: 2
attributes.tag: pcr_clean_up_methods
attributes.value: gel, column
attributes.tag: name_of_institute_creating_the_library
attributes.value: Xcelris Genomics, Ahmedabad, India
attributes.tag: sequencing_method_used
attributes.value: Illumina (MiSeq)
attributes.tag: targeted_gene_or_locus_name_for_marker_gene_studies
attributes.value: 16S rRNA gene
attributes.tag: url_of_instatute_sequencing_was_done
attributes.value: https://www.xcelrislabs.com/
attributes.tag: sequencing_direction
attributes.value: both
attributes.tag: name_of_subfragment_of_a_gene_or_locus
attributes.value: V4-V5
attributes.tag: list_of_forward_pcr_primers
attributes.value: AYTGGGYDTAAAGNG
attributes.tag: list_of_reverse_pcr_primers
attributes.value: CCGTCAATTCMTTTRAGT
attributes.tag: thermocycler_make_and_model
attributes.value: BIORAD, C1000
attributes.tag: tag_polymerase_used_for_amplification
attributes.value: Phusion High-Fidelity DNA Polymerase
attributes.tag: number_of_thermocycles
attributes.value: 28
attributes.tag: cycle_annealing_time_in_seconds
attributes.value: 30
attributes.tag: cycle_annealing_temperature_in_celsius
attributes.value: 55
attributes.tag: cycle_annealing_method
attributes.value: static
attributes.tag: cycle_denaturation_temperature_in_celsius
attributes.value: 98
attributes.tag: cycle_extension_temperature_in_celsius
attributes.value: 72
attributes.tag: initial_denaturation_temperature_in_celsius
attributes.value: 98
attributes.tag: final_extension_temperature_in_celsius
attributes.value: 72
attributes.tag: forward_primer_final_concentration_in_micromolar
attributes.value: 0.1
attributes.tag: reverse_primer_final_concentration_in_micromolar
attributes.value: 0.1
attributes.tag: datp_final_concentration_in_micromolar
attributes.value: 50
attributes.tag: dctp_final_concentration_in_micromolar
attributes.value: 50
attributes.tag: dgtp_final_concentration_in_micromolar
attributes.value: 50
attributes.tag: dttp_final_concentration_in_micromolar
attributes.value: 50
attributes.tag: mgcl2_final_concentration_expressed_in_millimolar
attributes.value: 2.5
attributes.tag: BioSampleModel
attributes.value: Metagenome or environmental