organism: Mus musculus
attributes.tag: sample_name
attributes.value: DRS002036
attributes.tag: sample comment
attributes.value: 24 nanoCAGE/CAGEscan libraries were prepared from total RNAs of Fluorescence-activated cell sorting (FACS) sorted parvalbumin-positive neurons ranging from 30-127 ng, where four treatments including two HDACi (TSA and VPA) with their vehicle controls, two time points after each treatment (2h and 2d) were compared. Three biological replicates for each combination of treatment and time point were prepared. In order to sequence multiplex libraries at a time, a six nucleotide barcode system was introduced. Briefly, the barcodes were introduced inside the template-switching primer and incorporated at the 5Ã¢ÂÂ²-end of the nanoCAGE libraries through reverse transcription. After cDNA synthesis, 24 libraries were pooled into single library and adapted for sequencing with an Illumina Genome analyzer IIx.