title: polyA+_liver_6100188
organism: Homo sapiens
description: Protocols: Human polyA+ RNAs were purchased from Clontech. The RNAs were isolated from tissue samples using a modified guanidium thiocynate extraction method followed by polyA+ RNA selection with two rounds of oligo(dT)-cellulose columns. First-strand cDNA samples were prepared from human poly(A)+ RNAs (BD-Clontech) with the SuperScript III kit (Invitrogen). Amplifications were performed in a final volume of 12.5 Î¼L with JumpStart REDTaq ReadyMix (Sigma-Aldrich) and a primer concentration of 0.4 Î¼M in 384-well plates format on an automatized Evoware platform (TECAN) combined with a Tetrad2 thermocycler (Bio-Rad) that allows processing four plates in parallel. Because monoexonic amplification is sensitive to genomic DNA contaminations, monoexonic models were assessed by amplification of cDNA in which a dNTP analog was incorporated using the mRNA Selective PCR Kit (TAKARA).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2014-12-16T17:02:45Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T22:09:49Z
attributes.tag: External Id
attributes.value: SAMEA3176229
attributes.tag: INSDC center name
attributes.value: Wellcome Trust Sanger Institute
attributes.tag: INSDC first public
attributes.value: 2014-12-16T17:02:45Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T22:09:49Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-3169:polyA+_liver_6100188
attributes.tag: biosource type
attributes.value: frozen sample
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: human
attributes.tag: organism part
attributes.value: liver
attributes.tag: sample name
attributes.value: E-MTAB-3169:polyA+_liver_6100188
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: male