title: Pooling-based genetic mapping of DNA methylation
organism: Homo sapiens
description: DNA methylation is an epigenetic modification that plays a key role in gene regulation. Previous studies have attempted to understand the role of methylation by mapping genetic variants that are associated with DNA methylation, but these have been limited to microarrays that cover less than 2% of the genome. We present a novel approach in which bisulfite-treated DNA from many individuals is sequenced together in a single pool, resulting in a truly genome-wide map of DNA methylation. Pooling samples increases statistical power to detect associations, while sharply reducing cost, effort, and experimental variability. As a proof of concept, we generated deep sequencing data from the pooled DNA of 60 human cell lines.
attributes.tag: isolate
attributes.value: YRI
attributes.tag: age
attributes.value: unknown
attributes.tag: biomaterial_provider
attributes.value: Coriell
attributes.tag: sex
attributes.value: not applicable
attributes.tag: tissue
attributes.value: LCL
attributes.tag: cell_line
attributes.value: LCL
attributes.tag: population
attributes.value: YRI
attributes.tag: treatment
attributes.value: bisulfite
attributes.tag: cell_lines
attributes.value: NA19239, NA19238, NA19223, NA19222, NA19210, NA19209, NA19207, NA19206, NA19203, NA19204, NA19200, NA19201, NA19192, NA19193, NA19171, NA19172, NA19160, NA19159, NA19153, NA19152, NA19144, NA19143, NA19141, NA19140, NA19138, NA19137, NA19130, NA19131, NA19128, NA19127, NA19119, NA19116, NA19101, NA19102, NA19098, NA19099, NA19092, NA19093, NA18913, NA18912, NA18871, NA18870, NA18862, NA18861, NA18859, NA18858, NA18856, NA18855, NA18853, NA18852, NA18522, NA18523, NA18516, NA18517, NA18507, NA18508, NA18504, NA18505, NA18501, NA18502
attributes.tag: BioSampleModel
attributes.value: Human