title: Sample 12
organism: Arabidopsis thaliana
description: Protocols: Seedlings were harvested after 14 days. WT A. thaliana Col-0 seeds were sown aseptically on MS10 plates, stratified for 2 days at 4 degrees C and then grown at a constant 21 degrees C under a 16-h light/8-h dark cycle for a further 14 days. Total RNA extraction with RNeasy Plant Mini Kit (Qiagen) then treated with TURBOTM DNase (Ambion). 4 μl of ERCC spike-ins at a 1:100 dilution were added to 1 μg/6 μl of total RNA. Illumina TruSeq Stranded Total RNA with Ribo-Zero Plant.
attributes.tag: ENA first public
attributes.value: 2019-02-08
attributes.tag: ENA last update
attributes.value: 2017-01-30
attributes.tag: External Id
attributes.value: SAMEA56658418
attributes.tag: INSDC center alias
attributes.value: Barton Group, Division of Computational Biology
attributes.tag: INSDC center name
attributes.value: Barton Group, Division of Computational Biology
attributes.tag: INSDC first public
attributes.value: 2019-02-08T17:03:48Z
attributes.tag: INSDC last update
attributes.value: 2017-01-30T12:01:26Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-5446:Sample 12
attributes.tag: age
attributes.value: 14
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: thale cress
attributes.tag: developmental stage
attributes.value: seedling
attributes.tag: ecotype
attributes.value: Col-0
attributes.tag: ercc spike-in mix
attributes.value: 1
attributes.tag: experiment
attributes.value: 2
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: sample name
attributes.value: E-MTAB-5446:Sample 12