title: XTE-from-XEN_Rep1
organism: Mus musculus
description: Protocols: Cells were collected from culture using standard procedures. XTE cells were obtained by either from ES cells or XEN cells or directly from the embryo by culturing them on feeders in an N2B27 basal medium containing Bmp4, CHIR99021, hFGF4, Heparin and Activin (XTE medium). The XTE cells were further selected by FACS sorting for cells expressing both PDGFRa and CD40 surface markers. Total RNA was isolated using Macherey-Nagel RNA isolation kit. 1ug of this was used for library preparation and sequencing. Enrichment of mRNA was performed using NEBNext® Poly(A) mRNA Magnetic Isolation Module and cDNA library was constructed using NEBNext® Ultra™ II Directional RNA Library Prep Kit.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2021-02-01T04:05:23Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-02-11T16:00:05Z
attributes.tag: External Id
attributes.value: SAMEA6535902
attributes.tag: INSDC center name
attributes.value: Max Planck Institute for Molecular Biomedicine
attributes.tag: INSDC first public
attributes.value: 2021-02-01T04:05:23Z
attributes.tag: INSDC last update
attributes.value: 2020-02-11T16:00:05Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8788:XTE-from-XEN_Rep1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: XTE cell
attributes.tag: cell type
attributes.value: extraembryonic cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: pre-implantation embryo
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: embryo
attributes.tag: progenitor cell type
attributes.value: XEN cell
attributes.tag: sample name
attributes.value: E-MTAB-8788:XTE-from-XEN_Rep1
attributes.tag: scientific_name
attributes.value: Mus musculus