title: XEN_Rep2
organism: Mus musculus
description: Protocols: Cells were collected from culture using standard procedures. XEN cells were derived from blastocyst by plating E3.5 embryos into individual wells of 96-well plate with feeders and cultured in DMEM medium containing 15% FBS, 2 mM L-glutamine, 1 mM sodium pyruvate, 0.1 mM non-essential amino acids, 50 U/ml penicillin-streptomycin, 0.1 mM 2mercaptoethanol. After 4 days the blastocyst outgrowths were trypsinised and transferred to new wells to allow the emergence of XEN cells. Total RNA was isolated using Macherey-Nagel RNA isolation kit. 1ug of this was used for library preparation and sequencing. Enrichment of mRNA was performed using NEBNext® Poly(A) mRNA Magnetic Isolation Module and cDNA library was constructed using NEBNext® Ultra™ II Directional RNA Library Prep Kit.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2021-02-01T04:05:23Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-02-11T16:00:05Z
attributes.tag: External Id
attributes.value: SAMEA6535894
attributes.tag: INSDC center name
attributes.value: Max Planck Institute for Molecular Biomedicine
attributes.tag: INSDC first public
attributes.value: 2021-02-01T04:05:23Z
attributes.tag: INSDC last update
attributes.value: 2020-02-11T16:00:05Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8788:XEN_Rep2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: XEN cell
attributes.tag: cell type
attributes.value: extraembryonic cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: pre-implantation embryo
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: embryo
attributes.tag: sample name
attributes.value: E-MTAB-8788:XEN_Rep2
attributes.tag: scientific_name
attributes.value: Mus musculus