title: Bisulfite-Seq of maize: wild type immature anthers (1.5-2.0 mm) replicate 2
organism: Zea mays
description: The immature anthers (1.5-2.0 mm) of the F1 individual B73/Mo17;+/+ were collected on the agricultural research farm at Miami University, flash-frozen in liquid nitrogen and preserved at -80. DNA was extracted from frozen tissue using the modified CTAB method. Due to the low amount of DNA, high-throughput low input whole genome bisulfite sequencing was performed at Novogene, and 150 bp paired end reads were generated.
attributes.tag: cultivar
attributes.value: B73/Mo17 hybrid
attributes.tag: age
attributes.value: 6 week old plants after planting
attributes.tag: dev_stage
attributes.value: Immature anthers (1.5-2.0 mm)
attributes.tag: geo_loc_name
attributes.value: USA: Ohio
attributes.tag: tissue
attributes.value: Immature anthers (1.5-2.0 mm)
attributes.tag: biomaterial_provider
attributes.value: Meixia Zhao
attributes.tag: collected_by
attributes.value: Meixia Zhao
attributes.tag: collection_date
attributes.value: 2020-07-25
attributes.tag: genotype
attributes.value: B73/Mo17;+/+
attributes.tag: sex
attributes.value: male
attributes.tag: replicate
attributes.value: biological replicate 2
attributes.tag: BioSampleModel
attributes.value: Plant