title: TEC_RIR_infected
organism: Gallus gallus
description: Protocols: Three groups comprising three indigenous, three hybrid and three commercial two day-old chicks were used in the experiment. These were used to obtain airway epithelial cells which were isolated from the trachea and lungs and cultured by the methods described by Zaffuto et al., (2008). The tracheal epithelial cells and lung epithelial cells from the three groups once adopted into culture were split into two groups each having three replicates. One group was infected with a Kenyan virulent strain of Newcastle disease virus with a multiplicity of infection (MOI) of 0.5 and the second group served as control. Both groups were incubated for 36 hours after which total RNA was obtained RNA was obtained after standard Trizol extraction Libraries were prepared as per manufacturer's instructions for the SOLiD total RNAseq kit
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2015-01-19T17:03:08Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T20:46:28Z
attributes.tag: External Id
attributes.value: SAMEA3110334
attributes.tag: INSDC center name
attributes.value: The Roslin Institute
attributes.tag: INSDC first public
attributes.value: 2015-01-19T17:03:08Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T20:46:28Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-3068:TEC_RIR_infected
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: trachea epithelial cell
attributes.tag: common name
attributes.value: chicken
attributes.tag: disease
attributes.value: Newcastle Disease
attributes.tag: ecotype
attributes.value: Rhode Island Red
attributes.tag: sample name
attributes.value: E-MTAB-3068:TEC_RIR_infected
attributes.tag: scientific_name
attributes.value: Gallus gallus