title: Macro5
organism: Mus musculus
description: Protocols: 5 x 10^5 B16F10-GFP cells were implanted subcutaneously into the footpad region of hind limb of 8~9-week-old male C57BL/6 mice. B16F10-GFP+ tumor cells from primary site (footpad) and pLNs (isolated at 3 weeks after implantation for micro-metastatic tumor with metastatic foci diameter less than 2 mm and at 4 weeks after implantation for macro-metastatic tumor) were isolated. Tissues were removed, digested, depleted CD45+ cells using AutoMACS, and GFP+ tumor cells were sorted by FACS Aria II. RNA was harvested using Trizol reagent (Invitrogen). 500 ng of total RNA was used for the construction of sequencing libraries. RNA libraries were prepared for sequencing using LEXOGEN Quant-Seq Library Prep Kit (Cat#001.24) standard protocols.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-02-09T17:02:21Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-01-24T13:50:51Z
attributes.tag: External Id
attributes.value: SAMEA5243226
attributes.tag: INSDC center name
attributes.value: Graduate School of Medical Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST)
attributes.tag: INSDC first public
attributes.value: 2019-02-09T17:02:21Z
attributes.tag: INSDC last update
attributes.value: 2019-01-24T13:50:51Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7621:Macro5
attributes.tag: age
attributes.value: 11 to 12
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: B16F10
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: LN macro-metastatic tumor rep2
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: tumor
attributes.tag: sample name
attributes.value: E-MTAB-7621:Macro5
attributes.tag: sampling site
attributes.value: LN macro-metastatic tumor
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: C57BL/6