title: F04_EA1
organism: Mus musculus
description: Protocols: FACS sorted cells from mice embryos 22-400 cells were directly sorted into 800μl Trizol® and frozen at -80oC. RNA was extracted in the aqueous phase after addition of 160ul chloroform, precipitated with an equal volume of isopropanol supplemented with 20μg linear polyacrylamide and washed once in fresh 80% ethanol. The RNA pellet was re-suspended in 3μl water at 50oC for 10 minutes and then kept on ice. RNA was reverse transcribed and amplified using SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing (634891) and libraries generated using Nextera® XT library preparation kit (Illumina, FC-131-1096).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2021-02-01T04:05:19Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-09-25T14:25:13Z
attributes.tag: External Id
attributes.value: SAMEA5972168
attributes.tag: INSDC center name
attributes.value: UCL Cancer Institute
attributes.tag: INSDC first public
attributes.value: 2021-02-01T04:05:19Z
attributes.tag: INSDC last update
attributes.value: 2019-09-25T14:25:13Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8362:F04_EA1
attributes.tag: age
attributes.value: 9.5
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: Ncx1-/-
attributes.tag: organism part
attributes.value: presumptive blood
attributes.tag: sample name
attributes.value: E-MTAB-8362:F04_EA1
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: strain
attributes.value: C57BL/6