title: A4
organism: Mus musculus
attributes.tag: strain
attributes.value: C57BL/6J
attributes.tag: isolate
attributes.value: To generate the HSE-FLPo transgenic mice, the HSE-FLPo fragment (2,301 bp) was excised using KpnI, SmaI and ScaI, and purified according to the standard protocol for microinjection. Prior to the injection, the 2,301 bp band was further verified with the expected 1,216 and 1,085 bp products of AhdI digestion. Microinjection into the C57BL/6J X C57BL/6J strain was performed by Cyagen. Founder line A4 was screened by PCR genotyping for FLPo.
attributes.tag: breed
attributes.value: HSE-FLPo founder line
attributes.tag: cultivar
attributes.value: NA
attributes.tag: ecotype
attributes.value: NA
attributes.tag: age
attributes.value: NA
attributes.tag: dev_stage
attributes.value: NA
attributes.tag: sex
attributes.value: female
attributes.tag: tissue
attributes.value: tail
attributes.tag: BioSampleModel
attributes.value: Model organism or animal