title: ARMS2
organism: Homo sapiens
description: Protocols: RMS tumour samples were obtained at diagnosis before any treatment from children admitted to the Department of Paediatrics and Infantile Neuropsychiatry at Sapienzu University of Rome, and to the Department of Oncology at Alder Hey Childrens NHS Trust, Liverpool. Histopathological diagnosis was confirmed using immunohistochemistry. Total RNA was extracted using Trizol reagent, according to the manufacturers instructions. Samples were enriched for small RNAs up to 200 bp by size selection using Pure Link miRNA Isolation Kit (LifeTech). Enriched RNA samples were processed using the Small RNA Expression Kit according to the manufacturers protocol (Small RNA expression kit, rev. C, Applied Biosystems). cDNA libraries were barcoded using the Solid RNA barcoding kit and amplified onto beads using emulsion PCR. Templated beads were deposited on slides and analysed using the Applied Biosystems SOLiD 4 Sequencer
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2015-01-28T17:02:44Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T23:07:50Z
attributes.tag: External Id
attributes.value: SAMEA3214820
attributes.tag: INSDC center name
attributes.value: Genomnia srl
attributes.tag: INSDC first public
attributes.value: 2015-01-28T17:02:44Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T23:07:50Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-3246:ARMS2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: alveolar rhabdomyosarcoma
attributes.tag: disease staging
attributes.value: Stage 3
attributes.tag: organism part
attributes.value: extremity
attributes.tag: sample name
attributes.value: E-MTAB-3246:ARMS2
attributes.tag: scientific_name
attributes.value: Homo sapiens