title: Transcriptome sample from Metarhizium anisopliae E6 - 144 h tick cuticle infection condition
organism: Metarhizium anisopliae
description: Rhipicephalus microplus cuticles were sterilized and used as the sole nutrient source for M. anisopliae growth and development. A spore suspension (5x10 6 spores per ml) was used to inoculate the cuticles by immersion for 30 sec. The inoculated cuticles were disposed over 1% water agar plates and maintained for 48 h and 144 h at 28C. Each of the two biological replicates consisted of a pool of five plates containing mycelium growth over the cuticles. The comparative control condition was conducted on 100 ml liquid complete medium for 48 h at 28C. The resulting fungal growth over the host cuticle and on liquid medium was ground to a powder in liquid nitrogen, and the total RNA was extracted using TRIzol Reagent (Life Technologies, CA, USA) following the manufacturer's instructions. The total isolated RNA was subjected to DNase treatment using RNase-free DNase I (Thermo Scientific, MA, USA). Large ribosomal RNA molecules were selectively depleted from the total RNA using a RiboMinus TM Eukaryote Kit for RNA-Seq (Life Technologies, CA, USA), and mRNA was concentrated and purified on a RiboMinus TM Concentration Module (Life Technologies, CA, USA).
attributes.tag: strain
attributes.value: E6
attributes.tag: collection_date
attributes.value: 1978
attributes.tag: geo_loc_name
attributes.value: Brazil: Espirito Santo State
attributes.tag: isolation_source
attributes.value: isolated from Deois flavopicta
attributes.tag: sample_type
attributes.value: missing
attributes.tag: BioSampleModel
attributes.value: Microbe, viral or environmental