title: MEF_CULTURE_INPUT
organism: Mus musculus
description: Protocols: hmeDIP-Seq was performed as previously described. Briefly, DNA was sonicated to yield fragmented DNA with a modal size of 150 bp. Following end-repair and A-tailing, Illumina TruSeq adaptors were added to the resulting DNA according to the manufacturer's instructions. The adaptor ligated DNA was then subjected to hmeDIP as outlined in associated manuscript. The resulting INPUT and hMeDIP samples were PCR amplified for fifteen cycles using single-end Illumina PCR primers. Finally, libraries were size selected by agarose gel electrophoresis. All DNA purification steps were carried out using Agencourt AMPure Beads (Beckman Coulter), and DNA quality, quantity and size was tested after every step on an Agilent Bioanalyser using High Sensitivity DNA chips (Agilent). Samples were subjected to single-end sequencing on the Illumina GAIIx platform. Sequencing was carried out at the Genomics Core, Albert Einstein College of Medicine, New York.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2014-12-19T17:02:55Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T22:02:04Z
attributes.tag: External Id
attributes.value: SAMEA3177776
attributes.tag: INSDC center name
attributes.value: Linkoping University, Linkoping, Sewden
attributes.tag: INSDC first public
attributes.value: 2014-12-19T17:02:55Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T22:02:04Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-3183:MEF_CULTURE_INPUT
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: mouse embryonic fibroblast cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: growth condition
attributes.value: cultured cell
attributes.tag: sample name
attributes.value: E-MTAB-3183:MEF_CULTURE_INPUT
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: female