title: ES_Rep3
organism: Mus musculus
description: Protocols: Cells were collected from culture using standard procedures. Mouse embryonic stem cells were maintained in the presence of 2i/Lif (0.4 µM PD0325901, 3 µM CHIR99021 and 4 ng/ml Lif), on plastic dishes coated with 0.2% gelatine. Total RNA was isolated using Macherey-Nagel RNA isolation kit. 1ug of this was used for library preparation and sequencing. Enrichment of mRNA was performed using NEBNext® Poly(A) mRNA Magnetic Isolation Module and cDNA library was constructed using NEBNext® Ultra™ II Directional RNA Library Prep Kit.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2021-02-01T04:05:23Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-02-11T16:00:05Z
attributes.tag: External Id
attributes.value: SAMEA6535886
attributes.tag: INSDC center name
attributes.value: Max Planck Institute for Molecular Biomedicine
attributes.tag: INSDC first public
attributes.value: 2021-02-01T04:05:23Z
attributes.tag: INSDC last update
attributes.value: 2020-02-11T16:00:05Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8788:ES_Rep3
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: mouse embryonic stem cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: pre-implantation embryo
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: embryo
attributes.tag: sample name
attributes.value: E-MTAB-8788:ES_Rep3
attributes.tag: scientific_name
attributes.value: Mus musculus