title: P9804_118
organism: Sus scrofa
description: Protocols: Three months after the MI procedure, the heart was excised and washed in saline and phosphate-buffered saline. After weighing the entire hearts, tissue samples (approximately 20 mg) were taken from the infarcted, border and remote areas for subsequent gene expression analysis and metabolomic analysis. Hearts were then sliced in 0.5 cm slices from apex to base. The slices were photographed on both sides for subsequent analysis in a computerized system (Imagescope, Aperio Technologies Inc). Control tissue was harvested from 3 separate naïve pigs not subjected to ischemia and reperfusion to induce MI. A catheter (Seldinger introducers) was placed in the right carotid artery for catheterization of the LAD. In the carotid artery introducer, a guide (GC Adroit, 6F, JR4, Johnson & Johnson, Sweden) were advanced to the aortic arch and by means of fluoroscopic guidance and injection of contrast agent (Visipaque 320, Apoteket, Sweden), the coronary vessels were visualized to enable positioning of a guide wire (PCI Wire, Wizdom SGW 0.014'' 300 J Soft, Johnson & Johnson, Sweden or Choice Floppy guide wire H74) in the LAD. A balloon catheter (Maverick2 Monorail 9-12 mm x 3.5 mm) was advanced over the wire and positioned distal to the first diagonal branch of the LAD. To induce ischemia and MI the balloon was inflated for 120 min. The pressure was selected according the specifications of the manufacturer (normally 6-9 ATM). After 120 min of occlusion, the balloon was deflated to initiate reperfusion and the catheter was withdrawn. In case severe ventricular arrhythmias appeared, attempts to restore sinus rhythm by means of ventricular defibrillation and chest compression as well as injection of lidocaine were initiated. When electrically and hemodynamically stable, the pig was sutured together and brought back to the pen to wake up. Post-surgery analgesia (flunixin, 2.2 mg/kg) was given intramuscularly for three days post-MI surgery maximum. Total RNA was isolated using RNeasy® Fibrous Tissue Mini Kit (Qiagen) according to the manufacturer's protocol. Sequencing library construction was performed using Illumina TruSeq stranded mRNA with Illumina RiboZero method.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2021-02-01T04:05:24Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-03-06T15:25:47Z
attributes.tag: External Id
attributes.value: SAMEA6611098
attributes.tag: INSDC center name
attributes.value: University of Skovde
attributes.tag: INSDC first public
attributes.value: 2021-02-01T04:05:24Z
attributes.tag: INSDC last update
attributes.value: 2020-03-06T15:25:47Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8856:P9804_118
attributes.tag: age
attributes.value: 1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: pig
attributes.tag: individual
attributes.value: 58
attributes.tag: injury
attributes.value: myocardial infarction
attributes.tag: organism part
attributes.value: heart left ventricle
attributes.tag: sample name
attributes.value: E-MTAB-8856:P9804_118
attributes.tag: sampling site
attributes.value: area bordering ischemia
attributes.tag: sampling time point
attributes.value: 3
attributes.tag: scientific_name
attributes.value: Sus scrofa
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: Gottingen minipig