organism: human gut metagenome
attributes.tag: host
attributes.value: Homo sapiens
attributes.tag: isolation_source
attributes.value: stool
attributes.tag: collection_date
attributes.value: 2012-11/2013-11
attributes.tag: geo_loc_name
attributes.value: Netherlands: Amsterdam, Maastricht, or Rotterdam
attributes.tag: lat_lon
attributes.value: 52.3676 N 4.9041 E
attributes.tag: samp_mat_process
attributes.value: Metagenomic DNA from 10 random post-travel samples were pooled together to construct a functional metagenomic library. The pooled metagenomic DNA was cloned into an expression vector and transformed into E. coli indicator hosts. These E. coli transformant libraries were then screened against piperacillin-tazobactam at selective concentration. Resistant clones were collected from solid media and were sequenced using Nextera protocol on Illumina NextSeq500.
attributes.tag: library_id
attributes.value: CBP09SR2
attributes.tag: antibiotic_name
attributes.value: piperacillin-tazobactam
attributes.tag: MIC (ug/ml)
attributes.value: 16-PI/4-TZ
attributes.tag: colony_count
attributes.value: 320
attributes.tag: time_point
attributes.value: Post-travel
attributes.tag: travel_destination_country
attributes.value: India
attributes.tag: travel_destination_region
attributes.value: Southern Asia
attributes.tag: BioSampleModel
attributes.value: Metagenome or environmental