title: ceacal contents of rat fed FEAM diet
organism: mouse gut metagenome
attributes.tag: sample_name
attributes.value: DRS012847
attributes.tag: sample comment
attributes.value: To clarify the effect of type of foods on the intestinal environment, Far East Asian (FEA, rich in rice starch, soy protein and soy oil) and Far East Asian marine (FEAM, rich in rice starch, fish meal, fish oil and brown alga) modelled diets, and sucrose, casein and beef tallow rich (SCB) diet were prepared. After 2-week administration of diets in rats, caecal organic acids and putrefactive compounds (ammonia, indole, phenol and H2S, which are regarded as putative risk factors for tumours) were determined. The caecal microbiota was also analyzed using DGGE and pyrosequencing with bar-coded primers targeting the bacterial 16S rRNA gene. Levels of n-butyrate, acetate, indole and phenol were high in rats fed FEA. On the other hand, H2S was clearly suppressed by both FEA and FEAM comparing with SCB. These results suggest that FEAM is preferable to FEA for optimal intestinal environment and host health. Both microbial analyses showed that the diversity of microbiota in the FEAM group was lower than in the other diet groups. Ratio of Firmicutes, Bacteroidetes and Proteobacteria in the SCB group was about 5: 4: 1. Firmicutes, particularly Lachnospiraceae, was promoted by FEA and FEAM.