title: Pooled (12 individuals) skin samples from Arctocephalus gazella
organism: Arctocephalus gazella
description: Total RNA from Arctocephalus gazella (Antarctic fur seal) was converted into double stranded cDNA libraries using Illumina's TruSeq Stranded protocol. Briefly, poly-A containing mRNA molecules were purified from the pooled total RNA using oligo dT beads. The mRNA was subsequently fragmented and reverse transcribed into cDNA with strand specificity. Adaptors and a single A base were attached to each fragment before purificiation and PCR enrichment in order to generate the final cDNA library. This was sequenced on one lane of an Illumina HiSeq 2000.
attributes.tag: breed
attributes.value: A gazella
attributes.tag: age
attributes.value: various
attributes.tag: sex
attributes.value: not determined
attributes.tag: tissue
attributes.value: skin
attributes.tag: biomaterial_provider
attributes.value: British Antarctic Survey
attributes.tag: BioSampleModel
attributes.value: Model organism or animal