title: P45608
organism: Homo sapiens
description: Protocols: The establishment of primary bronchial epithelial cells (PBECs) from human donors has been previously described (Strandberg, K.; Palmberg, L.; Larsson, K. Effect of formoterol and salmeterol on IL-6 and IL-8 release in airway epithelial cells. Respir. Med. 2007, 101, 1132-1139.) PBECs were exposure to PAMAMs Total RNA was extracted and purified from the cells and the samples were kept at -80C until further use. The mRNA Seq of polyadenylated transcripts from total RNA (samples are purified by poly-A enrichment before library creation using Illumina Tru-seq RNA) was used to profile nanoparticle treated and control samples at the Science for Life Laboratory, Stockholm, Sweden.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2015-01-17T17:01:23Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T22:40:52Z
attributes.tag: External Id
attributes.value: SAMEA3209347
attributes.tag: INSDC center name
attributes.value: Nanosafety & Nanomedicine Laboratory, Division of Molecular Toxicology, Institute of Environmental Medicine (IMM), Karolinska Institutet
attributes.tag: INSDC first public
attributes.value: 2015-01-17T17:01:23Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T22:40:52Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-2397:P45608
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: primary bronchial epithelial cell
attributes.tag: common name
attributes.value: human
attributes.tag: individual
attributes.value: D10
attributes.tag: organism part
attributes.value: lung
attributes.tag: sample name
attributes.value: E-MTAB-2397:P45608
attributes.tag: scientific_name
attributes.value: Homo sapiens