title: THP1_ctrl
organism: Homo sapiens
description: Protocols: OVCAR8 were obtained from NIH. THP-1 were obtained from ATCC. OVCAR-8 (2 x 10^5/12Well); cultured in RPMI1640 10 % FCS, 1% P/S over night; starvation in RPMI 1% BSA for 48 h; treatment Pretreatment: LPA-Mix LPA 16:0 (1 µM) LPA 18:0 (0,25 µM) LPA 18:1 (0,5 µM) LPA 18:2 (1,625 µM) LPA 20:4 (1,625 µM) (5 µM) for the denoted period of time NucleoSpin RNA Kit (Macherey-Nagel, 740955.250); DNA-free™ DNA Removal Kit (ThermoFisher/Ambion, AM1906); according to manufacturer's protocol Libraries were created using the Illumina Truseq mRNA kit v2 used according to the manufacturer's instructions.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-02-01T17:01:54Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-08-02T15:41:14Z
attributes.tag: External Id
attributes.value: SAMEA4812488
attributes.tag: INSDC center name
attributes.value: Institute for molecular and tumor biology Philipps University Marburg Germany
attributes.tag: INSDC first public
attributes.value: 2019-02-01T17:01:54Z
attributes.tag: INSDC last update
attributes.value: 2018-08-02T15:41:14Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7113:THP1_ctrl
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: THP-1
attributes.tag: cell type
attributes.value: monocyte
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: acute monocytic leukemia
attributes.tag: organism part
attributes.value: blood
attributes.tag: sample name
attributes.value: E-MTAB-7113:THP1_ctrl
attributes.tag: scientific_name
attributes.value: Homo sapiens