title: P29_l_02
organism: Mus musculus
description: Protocols: C57BL/6J mice were bred and housed under UK Home Office licensing husbandry standards. After breeding, mice were observed for plugs. Morning of day when plug was first observed was counted as day E0. Samples were prepared by direct perfusion of the tissue with buffered salt solution, followed by 1% formaldehyde cross-linking. After 20 minutes, the cross-linking reaction was quenched with 1/20 volume of 2.5 M glycine to neutralize the formaldehyde. The animal tissues were crosslinked with formaldehyde and chromatin fragmented to an average of 300 bp by sonication. Chromatin from an mass equivalent of 1/4 mouse liver was used for each ChIP experiment. Solexa libraries were prepared following the inst
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2014-08-08T17:01:13Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T17:12:17Z
attributes.tag: External Id
attributes.value: SAMEA2361895
attributes.tag: INSDC center name
attributes.value: Cambridge Institute
attributes.tag: INSDC first public
attributes.value: 2014-08-08T17:01:13Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T17:12:17Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-2326:P29_l_02
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: postnatal day 29
attributes.tag: organism part
attributes.value: liver
attributes.tag: phenotype
attributes.value: wild type
attributes.tag: sample name
attributes.value: E-MTAB-2326:P29_l_02
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: specimen with known storage state
attributes.value: fresh specimen
attributes.tag: strain
attributes.value: C57BL/6J