title: Sample 4
organism: Homo sapiens
description: Protocols: ER-alpha positive BC cells: MCF-7 (ATCC HTB-22), were propagated in Dulbecco's modified Eagle medium (DMEM; Sigma-Aldrich) supplemented with 10% FBS (HyClone) and antibiotics: 100 U/ml penicillin, 100 mg/ml streptomycin, 250 ng/ml amphotericin-B. None ER-alpha positive BC cells: MCF-7 (ATCC HTB-22) were propagated in Dulbecco's modified Eagle medium (DMEM; Sigma-Aldrich) supplemented with 10% FBS (HyClone) and antibiotics: 100 U/ml penicillin, 100 mg/ml streptomycin, 250 ng/ml amphotericin-B. 104cells/well were incubated with compounds for 3 or 6 days with media containing compounds or vehicle and in particular MCF-7 cells treated with EPZ004777 6.4µM or DMSO for for 3 and 6 days or with 4-hydroxytamoxifen 10-7 M (4-OHT) or fulvestrant 10-7 M (ICI 182,780) or DMSO for 3 days. None 104cells/well were incubated with compounds for 3 or 6 days with media containing compounds or vehicle and in particular MCF-7 cells treated with EPZ004777 6.4µM or DMSO for for 3 and 6 days or with 4-hydroxytamoxifen 10-7 M (4-OHT) or fulvestrant 10-7 M (ICI 182,780) or DMSO for 3 days. For RNA sequencing experiments, indexed libraries were prepared using 1 µg of total RNA from cells with TruSeq Stranded Total RNA Sample Prep Kit (Illumina Inc.).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-02-08T17:04:05Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-06-11T10:31:01Z
attributes.tag: External Id
attributes.value: SAMEA4721605
attributes.tag: INSDC center name
attributes.value: LabMedMolGe
attributes.tag: INSDC first public
attributes.value: 2019-02-08T17:04:05Z
attributes.tag: INSDC last update
attributes.value: 2018-06-11T10:31:01Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6875:Sample 4
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: MCF-7
attributes.tag: cell type
attributes.value: mammary epithelial cell
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: invasive ductal carcinoma
attributes.tag: metastatic site
attributes.value: pleural effusion
attributes.tag: organism part
attributes.value: mammary gland
attributes.tag: sample name
attributes.value: E-MTAB-6875:Sample 4
attributes.tag: scientific_name
attributes.value: Homo sapiens