title: Smith_Roslin_IBDV_inf_rep1.fq.gz
organism: Gallus gallus
description: Protocols: Birds were separated into two experimental rooms, with ad libitum access to food and water. In one room, each bird was mock-infected with 100 ul PBS intra-nasally. In the other room, each bird was infected intra-nasally with 101.3 embryonic infectious dose 50 (EID50) (in 100 µl PBS) of the virulent IBDV strain F52/70. Birds were monitored for clinical signs for 4 dpi and killed at 2, 3 and 4 dpi. Spleen and bursa were collected from all birds. Tissue samples were stabilized in RNAlater (Qiagen, Crawley, UK) and disrupted using a bead mill (Retsch MM 300, Retsch, Haan, Germany) at 20 Hz for 4 min. Total RNA was prepared using a Trizol (Invitrogen, Paisley, UK) extraction method as per the manufacturer's protocol. Samples were resuspended in a final volume of 100 ul of RNAse-free water. Samples were then passed through RNeasy columns (Qiagen) to clean up the RNA further. Concentrations of the samples were calculated by measuring OD260 and OD280 on a spectrophotometer. Quality of the RNA was checked on a bioanalyser (Agilent Technologies, South Queensferry, UK). Samples were prepared for mRNA sequencing using 5ug of total RNA starting material following the Illumina mRNA sequencing 8 sample preparation kit protocol.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2015-01-19T17:03:08Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T20:52:53Z
attributes.tag: External Id
attributes.value: SAMEA2757650
attributes.tag: INSDC center name
attributes.value: The Roslin Institute
attributes.tag: INSDC first public
attributes.value: 2015-01-19T17:03:08Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T20:52:53Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-2910:Smith_Roslin_IBDV_inf_rep1.fq.gz
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: chicken
attributes.tag: infect
attributes.value: IBDV
attributes.tag: organism part
attributes.value: spleen
attributes.tag: sample name
attributes.value: E-MTAB-2910:Smith_Roslin_IBDV_inf_rep1.fq.gz
attributes.tag: scientific_name
attributes.value: Gallus gallus
attributes.tag: time
attributes.value: 4 day