title: 723_2_TGACCA_L001_DF1
organism: Gallus gallus
description: Protocols: Chicken bone marrow derived macrophage (BMDM) culture: RNA from chicken BMDM, day 7 embryos, or DF-1 cells, was extracted using TRIzol reagent, as described by the manufacturers (Invitrogen). RNA was quantified using a Nanodrop and RNA quality was assessed using Agilent nanochip Samples were prepared for mRNA sequencing using 1ug of total RNA starting material following the Illumina Truseq RNA Sequencing protocol. Resulting libraries were quality checked on an Agilent DNA 1000 bioanalyzer (Agilent Technologies, South Queensferry, UK) and then clustered onto a paired read flowcell using the Illumina v2 cluster generation kit at a 8pM concentration.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2015-01-19T17:03:08Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T20:42:11Z
attributes.tag: External Id
attributes.value: SAMEA3109053
attributes.tag: INSDC center name
attributes.value: The Roslin Institute
attributes.tag: INSDC first public
attributes.value: 2015-01-19T17:03:08Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T20:42:11Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-3048:723_2_TGACCA_L001_DF1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: DF-1 cell
attributes.tag: common name
attributes.value: chicken
attributes.tag: sample name
attributes.value: E-MTAB-3048:723_2_TGACCA_L001_DF1
attributes.tag: scientific_name
attributes.value: Gallus gallus