title: Input
organism: Homo sapiens
description: Input, 1 micromolar dexamethasone for 90 minutes Protocols: Approximately 10 million cells were treated with 0.01% ethanol vehicle or 1 uM dexamethasone for 1.5 h. Chromatin was sheared with a Bio-ruptor water bath sonicator (Diagenode) to produce fragments of 100-200 bp. Protein-G coupled magnetic beads (Active- Motif) were preincubated for 1 h with GR-antibody (N499) before chromatin was added and incubated for an additional 2-4 h while rotating at 4C. Subsequently beads were washed three times with 10 mM TrisaHCl pH 8.0, 1 mM EDTA, 500 mM NaCl, 5% (vol/vol) glycerol, 0.1% sodium deoxycholate, 0.1% SDS, 1% Triton X-100, 0.5 mg/uL BSA, followed by three additional washes with 20 mM Tris, pH 8.0, 1 mM EDTA, 250 mM LiCl, 0.5% Nonidet P-40, and 0.5% sodium deoxycholate.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2014-08-08T17:01:15Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T18:48:52Z
attributes.tag: External Id
attributes.value: SAMEA2633139
attributes.tag: INSDC center name
attributes.value: EBI
attributes.tag: INSDC first public
attributes.value: 2014-08-08T17:01:15Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T18:48:52Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-2731:Input
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: U2OS
attributes.tag: common name
attributes.value: human
attributes.tag: sample name
attributes.value: E-MTAB-2731:Input
attributes.tag: scientific_name
attributes.value: Homo sapiens