title: Sample 5
organism: Homo sapiens
description: Protocols: Samples were cells in culture, centrifuged and pellets collected RNA was extracted from cell pellets using Trizol as described by the manufacturer. RNA degradation and contamination were monitored on 1% agarose gels and RNA integrity was assessed with the RNA Nano 6000 Assay Kit and a Bioanalyzer 2100 system For the infection studies triplicates of proneural GSCs (G44) cultured in suspension in neurobasal medium plus B27 supplement in 24 well plates were infected with HCMV Towne strain at a multiplicity of infection of 0.1. Cells were then kept in culture for three days. Uninfected cells served as controls. The libraries were prepared from 500ng of total RNA using Illumina TruSeq strand specific mRNAseq library prep kit according to manufacturer's protocol.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-02-08T17:02:46Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-01-23T12:57:03Z
attributes.tag: External Id
attributes.value: SAMEA5240984
attributes.tag: INSDC center name
attributes.value: Brigham and Womens Hospital
attributes.tag: INSDC first public
attributes.value: 2019-02-08T17:02:46Z
attributes.tag: INSDC last update
attributes.value: 2019-01-23T12:57:03Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7613:Sample 5
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: NCE-G 44
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: glioblastoma
attributes.tag: organism part
attributes.value: brain
attributes.tag: sample name
attributes.value: E-MTAB-7613:Sample 5
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: female