title: E. coli O157:H7 growth in D-serine2
organism: Escherichia coli O157:H7 str. EDL933
attributes.tag: Alias
attributes.value: E. coli O157:H7 growth in D-serine2
attributes.tag: Description
attributes.value: Starter cultures were grown overnight at 37˚C in LB and then used to inoculate MEM-HEPES (Sigma) + 1mM D-serine with growth @ 37˚C with shaking (200rpm) until late exponential phase. Total RNA was prepared as described previously (Tree et al., 2009; doi:10.1128/IAI.00562-09) and depleted for ribosomal sequences using a Microbexpress kit (Ambion). Sequencing of cDNA was carried out on an Illumina Genome Analyser IIx using single ended reads and 6 samples per lane.
attributes.tag: ENA checklist
attributes.value: ERC000011
attributes.tag: INSDC center alias
attributes.value: UQ
attributes.tag: INSDC center name
attributes.value: University of Queensland
attributes.tag: INSDC first public
attributes.value: 2014-12-12T09:24:03Z
attributes.tag: INSDC last update
attributes.value: 2014-12-11T04:13:35Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: SRA accession
attributes.value: ERS627412
attributes.tag: Sample Name
attributes.value: ERS627412
attributes.tag: Title
attributes.value: E. coli O157:H7 EDL933 TUV93-0 growth in 1 mM D-serine
attributes.tag: strain
attributes.value: EDL933
attributes.tag: sub_strain
attributes.value: TUV93-0