title: DRS_PNT_CHICK3
organism: Gallus gallus
attributes.tag: Alias
attributes.value: DRS_PNT_CHICK3
attributes.tag: DRS Sequencing
attributes.value: Direct sequencing of the unamplified RNA was undertaken by Helic Biosciences, as described in Ozsolak & Milos (2011) doi: 10.1002/wrna.84
attributes.tag: Description
attributes.value: DRS sequencing of Pre-Neutal Tube tissue microdissected from single chick embryos.
attributes.tag: ENA checklist
attributes.value: ERC000011
attributes.tag: INSDC center alias
attributes.value: DUNDEE
attributes.tag: INSDC center name
attributes.value: University of Dundee
attributes.tag: INSDC first public
attributes.value: 2014-08-01T17:01:10Z
attributes.tag: INSDC last update
attributes.value: 2014-07-10T21:48:02Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Microdissection
attributes.value: Dissections were carried out in L15 medium at 4°C. RNA was extracted in Trizol reagent (Invitrogen) by phase separation with chloroform, followed by precipitation with isopropanol and linear acrylamide. The RNA was washed in 70% ethanol, air-dried, resuspended in DEPCwater and frozen in liquid nitrogen. Total RNA was quantified and quality tested using the Agilent RNA assay (Agilent Bioanalyser pico RNA chip) by ARK-Genomics. Samples with a RIN number above 8.0 where selected. Notochord was removed by controlled trypsin digestion that aimed to keep the neural ventral midline.
attributes.tag: SRA accession
attributes.value: ERS500964
attributes.tag: Sample Name
attributes.value: ERS500964
attributes.tag: Title
attributes.value: PNT DRS Individual 3
attributes.tag: cell_type
attributes.value: Neural Tube
attributes.tag: collected_by
attributes.value: Olivera-Martinez
attributes.tag: dev_stage
attributes.value: HH10
attributes.tag: sex
attributes.value: male
attributes.tag: tissue_type
attributes.value: Pre-neural Tube