title: Sample 5
organism: mixed sample
description: Protocols: Three pools of three root tips per plant (~15 mm in length) were harvested and immediately snap-frozen in liquid N Plants were cultivated in vitro on McCown Woody Plant Medium (Duchefa) supplemented with 30 g L-1 sucrose, 0.27 µM 1-naphthalene acetic acid and 0.4 % agar in a growth chamber at 22 °C and with a photoperiod of 16 h light/8 h dark at a photon flux density of 55 µmol m-2 s-1. Six-week-old plantlets were then acclimated for four weeks to perlite-filled pots, irrigated with water, in a growth chamber at 26 °C and with a photoperiod of 16 h light/8 h dark at a photon flux density of 145 µmol m-2 s-1. Plants were then transferred into hydroponic culture with an aerated solution; each pot contained 2 plants of the same scion/rootstock combination with 700 mL of high P solution (HP, 0.6 mM P). Four days later, the pots were subjected to a low P (LP, 0.001 mM P) treatment. The macronutrient composition was 2.45 mM KNO3, 0.69 mM MgSO4 and 1.27 mM CaCl2 and also contained 0.3 mM K2SO4 and 0.3 mM CaSO4. Micronutrients were supplied as 46 μmol H3BO3, 9.1 μmol MnCl2, 2.4 μmol ZnSO4, 0.5 μmol CuSO4 and 14 nmol (NH4)6Mo7O24, and iron was supplied as 8.5 mg L-1 Sequestrene 138 (i.e. 31.3 μmol ethylenediamine-N,N′-bis (2-hydroxyphenylacetic acid) NaFe, Syngenta Agro S.A.S.). Total RNA of samples was extracted using the Spectrum Plant Total RNA kit (Sigma-Aldrich) with some modifications as previously described (Cookson and Ollat 2013) RNA sequencing libraries were generated from 500 ng of total RNA using TruSeq Stranded mRNA LT Sample Preparation Kit (Illumina), according to manufacturer's instructions
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-02-26T04:06:35Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-01-15T16:22:57Z
attributes.tag: External Id
attributes.value: SAMEA6475091
attributes.tag: INSDC center name
attributes.value: University of California, Davis, USA
attributes.tag: INSDC first public
attributes.value: 2020-02-26T04:06:35Z
attributes.tag: INSDC last update
attributes.value: 2020-01-15T16:22:57Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8678:Sample 5
attributes.tag: age
attributes.value: 10
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cultivar
attributes.value: 1103P grafted on 1103P
attributes.tag: genotype
attributes.value: Vitis berlandieri x Vitis rupestris cv 1103P grafted on Vitis berlandieri x Vitis rupestris cv 1103P
attributes.tag: grafting
attributes.value: Vitis berlandieri x Vitis rupestris grafted on Vitis berlandieri x Vitis rupestris
attributes.tag: growth condition
attributes.value: hydroponics LP
attributes.tag: organism part
attributes.value: root tip
attributes.tag: sample name
attributes.value: E-MTAB-8678:Sample 5
attributes.tag: scientific_name
attributes.value: mixed sample