organism: human gut metagenome
attributes.tag: host
attributes.value: Homo sapiens
attributes.tag: isolation_source
attributes.value: stool
attributes.tag: collection_date
attributes.value: 2012-11/2013-11
attributes.tag: geo_loc_name
attributes.value: Netherlands: Amsterdam, Maastricht, or Rotterdam
attributes.tag: lat_lon
attributes.value: 52.3676 N 4.9041 E
attributes.tag: samp_mat_process
attributes.value: Metagenomic DNA from 10 random post-travel samples were pooled together to construct a functional metagenomic library. The pooled metagenomic DNA was cloned into an expression vector and transformed into E. coli indicator hosts. These E. coli transformant libraries were then screened against cefotaxime at selective concentration. Resistant clones were collected from solid media and were sequenced using Nextera protocol on Illumina NextSeq500.
attributes.tag: library_id
attributes.value: CBP12SR2
attributes.tag: antibiotic_name
attributes.value: cefotaxime
attributes.tag: MIC (ug/ml)
attributes.value: 8
attributes.tag: colony_count
attributes.value: 1
attributes.tag: time_point
attributes.value: Post-travel
attributes.tag: travel_destination_country
attributes.value: Tanzania
attributes.tag: travel_destination_region
attributes.value: Eastern Africa
attributes.tag: BioSampleModel
attributes.value: Metagenome or environmental