title: GA2OX:PLA1_day6_1
organism: Zea mays
description: Protocols: Plants for leaf growth monitoring were grown under growth chamber conditions with controlled relative humidity (55%), temperature (24°C day/18°C night), and light intensity (170 mmol per m2 per second photosynthetic active radiation at plant level) provided by a combination of high-pressure sodium vapor (RNP-T/LR/400W/S/230/E40; Radium) and metal halide lamps with quartz burners (HRI-BT/400W/D230/E40; Radium) in a 16h/8h (day/night) cycle. Total RNA was isolated with the guanidinium thiocyanate-phenol-chloroform extraction method using TRI-reagent (Sigma-Aldrich) Library preparation was done using the TruSeq RNA Sample Preparation Kit v2 (Illumina). In brief, poly(A)-containing mRNA molecules were reverse transcribed, double-stranded cDNA was generated and adapters were ligated. After quality control using a 2100 Bioanalyzer (Agilent), clusters were generated through amplification using the TruSeq PE Cluster Kit v3-cBot-HS kit (Illumina), followed by sequencing on an Illumina HiSeq2000 with the TruSeq SBS Kit v3-HS (Illumina).
attributes.tag: ENA first public
attributes.value: 2017-02-04
attributes.tag: ENA last update
attributes.value: 2017-01-24
attributes.tag: External Id
attributes.value: SAMEA54081418
attributes.tag: INSDC center alias
attributes.value: Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, Belgium VIB Center for Plant Systems Biology,VIB, Gent, Belgium
attributes.tag: INSDC center name
attributes.value: Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, Belgium VIB Center for Plant Systems Biology,VIB, Gent, Belgium
attributes.tag: INSDC first public
attributes.value: 2017-02-04T17:03:15Z
attributes.tag: INSDC last update
attributes.value: 2017-01-24T13:32:48Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-5422:GA2OX:PLA1_day6_1
attributes.tag: age
attributes.value: 6
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: genotype
attributes.value: GA2OX:PLA1
attributes.tag: organism part
attributes.value: basal half cm of growing leaf 4
attributes.tag: sample name
attributes.value: E-MTAB-5422:GA2OX:PLA1_day6_1
attributes.tag: strain
attributes.value: B104