title: soil_cDNA_FW_spring
organism: soil metagenome
description: cDNA of 16S rRNA from soil irrigated with FW at the end of spring
attributes.tag: ENA first public
attributes.value: 2014-08-08
attributes.tag: ENA last update
attributes.value: 2016-10-21
attributes.tag: External Id
attributes.value: SAMEA2586301
attributes.tag: INSDC center alias
attributes.value: ARO-VOLCANI
attributes.tag: INSDC center name
attributes.value: Agricultural Research Organization, Volcani Center, Israel
attributes.tag: INSDC first public
attributes.value: 2014-08-08T17:01:15Z
attributes.tag: INSDC last update
attributes.value: 2016-10-21T09:33:19Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: Kedma_16s rRNA_cDNA_60
attributes.tag: collection date
attributes.value: 2011-03-20
attributes.tag: depth
attributes.value: 0.1
attributes.tag: environment (biome)
attributes.value: olive orchard
attributes.tag: environment (feature)
attributes.value: Bulk soil
attributes.tag: environment (material)
attributes.value: soil
attributes.tag: geographic location (country and/or sea)
attributes.value: Israel
attributes.tag: geographic location (elevation)
attributes.value: 200
attributes.tag: geographic location (latitude)
attributes.value: 31.69804
attributes.tag: geographic location (longitude)
attributes.value: 34.76554
attributes.tag: investigation type
attributes.value: metagenome
attributes.tag: nucleic acid extraction
attributes.value: rRNA extraction was performed using methods previously described by Angel, R., Claus, P., and Conrad, R. (2011) Methanogenic archaea are globally ubiquitous in aerated soils and become active under wet anoxic conditions. ISME j. 6: 847-862
attributes.tag: pcr primers
attributes.value: 16S bacterial primers, 530F, GTG CCA GCM GCN GCG G and 1100R, GGG TTN CGN TCG TTG
attributes.tag: project name
attributes.value: kedma cDNA
attributes.tag: sample name
attributes.value: Kedma_16s rRNA_cDNA_60
attributes.tag: sequencing method
attributes.value: 454 tag-encoded FLX 16S rDNA amplicon pyrosequencing (bTEFAP)
attributes.tag: soil environmental package
attributes.value: soil