title: SCR_1
organism: Homo sapiens
description: Protocols: PLC5 cells were infected with polybrene (8µg/ml) and lentiviruses expressing either an empty vector (pLKO.1) or a shRNA against MAGEA3 (pLKO.1) from Sigma-Aldrich: shMAGEA3 #8375: TRCN00000128375. Cells were collected 72 hours after infection and used for RNA extraction. Lentivirus was diluted 1:2 for the RNA sequencing experiment. PLC5 cells were infected with polybrene (8µg/ml) and lentiviruses expressing either an empty vector (pLKO.1) or a shRNA against MAGEA3 (pLKO.1) from Sigma-Aldrich: shMAGEA3 #8375: TRCN00000128375. Cells were collected 72 hours after infection and used for RNA extraction. Lentivirus was diluted 1:2 for the RNA sequencing experiment. RNA from cell lines was extracted using the RNeasy mini kit (Qiagen). Libraries were constructed using the TruSeq RNA Library Prep Kit v2.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2021-02-01T04:05:24Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-03-09T11:51:06Z
attributes.tag: External Id
attributes.value: SAMEA6615076
attributes.tag: INSDC center name
attributes.value: Division of Liver Diseases, Department of Medicine, Liver Cancer Program, Tisch Cancer Institute, Graduate School of Biomedical Sciences, Icahn School of Medicine at Mount Sinai, New York (US).
attributes.tag: INSDC first public
attributes.value: 2021-02-01T04:05:24Z
attributes.tag: INSDC last update
attributes.value: 2020-03-09T11:51:06Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8860:SCR_1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: PLC/PRF/5
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: hepatocellular carcinoma
attributes.tag: organism part
attributes.value: liver
attributes.tag: sample name
attributes.value: E-MTAB-8860:SCR_1
attributes.tag: scientific_name
attributes.value: Homo sapiens