title: Kaiser_heterophil_control
organism: Gallus gallus
description: Protocols: Macrophages and dendritic cells were cultured from bone marrow by standard methods (Garceau et al. 2010 and Wu et al. 2010, respectively), and heterophils isolated from blood by standard methods (Kogut et al. 1995). Cells were cultured with or without LPS for 24 h before RNA was isolated Standard Trizol extraction method Samples were prepared for mRNA sequencing using 2ug of total RNA starting material following the Illumina TruSeq RNA Sample Preparation v2 kit protocol.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2015-01-19T17:03:08Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T20:12:40Z
attributes.tag: External Id
attributes.value: SAMEA2812695
attributes.tag: INSDC center name
attributes.value: The Roslin Institute
attributes.tag: INSDC first public
attributes.value: 2015-01-19T17:03:08Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T20:12:40Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-2996:Kaiser_heterophil_control
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: heterophil
attributes.tag: common name
attributes.value: chicken
attributes.tag: sample name
attributes.value: E-MTAB-2996:Kaiser_heterophil_control
attributes.tag: scientific_name
attributes.value: Gallus gallus