title: Sample 3
organism: Homo sapiens
description: Protocols: Samples were cells in culture, centrifuged and pellets collected RNA was extracted from cell pellets using Trizol as described by the manufacturer. RNA degradation and contamination were monitored on 1% agarose gels and RNA integrity was assessed with the RNA Nano 6000 Assay Kit and a Bioanalyzer 2100 system The libraries were prepared from 500ng of total RNA using Illumina TruSeq strand specific mRNAseq library prep kit according to manufacturer's protocol.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-02-08T17:02:46Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-01-23T12:57:02Z
attributes.tag: External Id
attributes.value: SAMEA5240982
attributes.tag: INSDC center name
attributes.value: Brigham and Womens Hospital
attributes.tag: INSDC first public
attributes.value: 2019-02-08T17:02:46Z
attributes.tag: INSDC last update
attributes.value: 2019-01-23T12:57:02Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7613:Sample 3
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: NCE-G 44
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: glioblastoma
attributes.tag: organism part
attributes.value: brain
attributes.tag: sample name
attributes.value: E-MTAB-7613:Sample 3
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: female