title: Old Eucalyptus replicate 4
organism: soil metagenome
description: Old Eucalyptus replicate 4
attributes.tag: isolation_source
attributes.value: soil
attributes.tag: collection_date
attributes.value: 2017-03-30
attributes.tag: geo_loc_name
attributes.value: Brazil: Belo Oriente, Cenibra, Old Eucalyptus 4
attributes.tag: lat_lon
attributes.value: 19 S 42.00004 W
attributes.tag: rel_to_oxygen
attributes.value: aerobe
attributes.tag: samp_collect_device
attributes.value: Steel tube
attributes.tag: samp_mat_process
attributes.value: Soil samples were taken from five points in each treatment area (YE, OE and NF), approximately 10 cm away from each gas flux chamber, resulting in five replicates per treatment. To extract each soil sample, we placed a steel tube probe of 1.5 cm diameter (previously sterilized at 180 C for 3 h to remove contaminants and nucleases) into the soil to a depth of 7 cm. The harvested soil was immediately put in a sterile 50 mL propylene tube for mixing, before being separated into two subsamples and placed in liquid nitrogen until we conducted DNA extractions.
attributes.tag: samp_size
attributes.value: 10g
attributes.tag: source_material_id
attributes.value: eucalyptus soil
attributes.tag: replicate
attributes.value: Biological Replicate 4
attributes.tag: BioSampleModel
attributes.value: Metagenome or environmental