title: Anlotinib_resistant_NCI-H1975_control_GTGGCC_L003_R1.fastq
organism: Homo sapiens
description: Protocols: The cells were cultured in RPMI 1640 medium (Gibco, USA) supplement with 10% FBS (Gibco, USA), and 0.1 mg/ml streptomycin, and 100 U/ml penicillin. All cells were incubated at 37℃, 5% CO2 in a humidified incubator. Cells cultured in 10 cm diametre dish. 1 ml trizol reagent (Life Technologies, Inc., USA) was used to lyse cell samples. Total RNA isolation using standard procedures.Oligotex mRNA Mini Kit (Qiagen, Germany) was performed to collect pure mRNA. About 100 ng mRNA of each sample was used to library. The library procedure includes cDNA reverse-transcription, end repair, ligation, and PCR amplification.