title: JP726
organism: Mus musculus
description: Protocols: The dams and their litters were randomly assigned to control or PAT groups. At postnatal (P) day 23, the pups were weaned and housed to separate males and females. All mice received acidified drinking water supplied by the facility routinely except for the periods when some litters were receiving antibiotic treatment. A therapeutic dose of the macrolide tylosin tartrate (Sigma-Aldrich, Billerica MA) was given to mice in their non-acidified drinking water with 333 mg/L (about 50 mg/kg body weight/day) on P5-10 for 1PAT mice or in three courses (P10-15, P28-31, and P37-40) (3PAT), exactly as described in (Livanos et al., 2016). Tylosin tartrate was dissolved in non-acidified water at a concentration of 333 mg/L. Control mice received non-acidified water, and PAT1 groups received one tylosin course at day 5 of life for 5 days. No cell cultures were used. RNA was extract from whole ileal tissue. RNA was extracted using the Qiagen miRNAeasy kit (Qiagen, Valencia CA) using the standard protocol. Ribosomal RNA was removed using the Ribo-Zero rRNA depletion kit (Illumina, San Diego CA). The TruSeq RNA sample preparation kit (Illumina, San Diego, CA) was used to prepare cDNA libraries from RNA samples.