title: Seedling-shoot-8 days-dark-treated-replicate-1
organism: Hordeum vulgare subsp. vulgare
description: Protocols: The growth chamber was set to cycle between 20C for 12 hours and 18C for the next 12 hours, and the soil was kept moist by gently spraying with water every 72 hours. Continuous dark: Samples harvested after growing them in continuous dark for 8 days (8DD) after germination. All plants germinated in 2 days. Therefore, dark samples were harvested 10 days after germination (10DAG). Total RNA from frozen seedling shoot sample was extracted as per manufacturer's protocol using RNA Plant reagent (Invitrogen Inc., USA), RNeasy kits (Qiagen Inc., USA), and treated with RNase-free DNase (Life Technologies Inc., USA) True-Seq kit (Illumina Inc.) was used to prepare RNA-seq libraries, according to the manufacture's protocol.