title: ingWAT_Cold_4
organism: Mus musculus
description: Protocols: Standard conditions (RT): The mice used in the experiments were C57BL/6 male mice bought from Charles River. The mice were kept at 21 °C until 8 weeks of age. Cold acclimation: At the start of the experiment, 8-week-old mice were single-caged and successively acclimated to cold by first placing them at 18 °C for 1-2 weeks and then at 4 °C for the following 4 weeks. So the mice at experiment are 13-14 weeks old. Samples are directly harvest and snap frozen Trizol Truseq total RNA