title: Sample 4
organism: Vibrio natriegens NBRC 15636 = ATCC 14048 = DSM 759
description: Protocols: Cells were cultivated in BHI media supplemented with 1.5 % sodium chloride for 24 h from a single colony. 0.7 ml of the overnight culture were pelleted and the pellet was used for genomic DNA preparation. Cells were grown over night starting from a single colony in BHIN at 30°C. Genomic DNA was extracted according to manufactory protocol using the NucleoSpin® Microbial DNA Kit from Macherey Nagel. The libraries were constructed using the TruSeq DNA PCR-free Kit from illumina.