title: COLO205_IP_Ab1_3
organism: Homo sapiens
description: Protocols: Cells were cultured under cell-line-specific conditions following the manufacturer's instructions RNA immunoprecipitation of PIWIL1 associated small RNAs was performed by overnight incubation with two different primary antibodies or without antibody addition, then secondary antibody conjugated-beads were added to collect the PIWIL1/RNA complexes. Total RNA was extracted by input and immunopurified samples using the mirVana PARIS Isolation Kit (Ambion Inc.) Small RNA library were prepared with TruSeq small RNA Sample Prep Kit (Illumina)