organism: Homo sapiens
description: Vero cells (ATCC, CCL-81) were maintained in DMEM (Gibco) and primary astrocytes were maintained in DMEM/F12 (Gibco). ZIKV strain PE/243 (GenBank accession #KX197192.1). Viral stocks used here ZIKV (2.6 x 106 PFU/ml) were propagated by serial passages in Vero cells by routine methods using DMEM. Astrocyte infections were performed at MOI 1 (2 x 105 cells/well) for all four viruses in 12 well plate during 1 h at 37 C and 5% CO2 in medium without FBS.