title: Mut8
organism: Mus musculus
description: Protocols: The striatums were dissected out, and both hemispheres were pooled together. Mutant embryos were produced through homologous recombination in ES cells; replacing the CDS of Zfp503 (Nolz1) with β-galactosidase. RNA was extracted using the RNeasy micro kit from Qiagen. Total RNA was treated to remove ribosomal RNA using the Illumina RiboZero Gold (Human/Mouse/Rat) kit, according to the manufacturer's protocol. 100-200ng of the ribo-depleted RNA was used to make sequencing libraries, using the Illumina TruSeq Stranded mRNA kit, according to the manufacturer's protocol.