organism: soil metagenome
description: Spartina alterniflora and Juncus roemerianus were transplanted in planting plots , spaced 30 cm apart and planted 10 cm deep with a total of 64 plugs for each planting plot.  Slow-release fertilizer (Osmocote Plus with 15% N, 8% P2O5 and 11% K2O) was applied twice a year (April and September) in fertilizing plots at a rate of 326 kg N/ha/yr, 76 kg P/ha/yr and 198 kg K/ha/yr.  Fertilizer was inserted 10 cm beneath the soil surface to promote efficiency of nutrient release and to minimize leaching.  For plots receiving nutrient, Spartina, and Juncus amendments, fertilizer was added below transplanted Spartina and Juncus roots.