organism: Homo sapiens
description: Vero cells (ATCC, CCL-81) were maintained in DMEM (Gibco) and primary astrocytes were maintained in DMEM/F12 (Gibco). OROV strain BeAn19991 (GenBank accession #KP052852.1, #KP052851.1, #KP052850.1) . Viral stocks used here OROV  (2 x 106 PFU/ml)  were propagated by serial passages in Vero cells by routine methods using DMEM. Astrocyte infections were performed at MOI 1 (2 x 105 cells/well) for all four viruses in 12 well plate during 1 h at 37 C and 5% CO2 in medium without FBS.