title: PDE5
organism: Mus musculus
description: Protocols: Th-MYCNCPM32-model is based on 129X1/SvJ-Tg(TH-MYCN)41Waw/Nci. Homozygote tumour bearing mice for MYCN were subject to individualized dose escalation schedule for cyclophosphamide (CPM) until they acquired resistance to 32mg/kg CPM. Mice included in the study were sacrificed and primary tumours were dissected out. Tumour samples were sent to a commerical sequencing unit which extracted the RNA. TruSeq® Stranded Total RNA according to manufacturers protocol