title: dKD2 rep1 nucRNA
organism: Homo sapiens
description: Protocols: HeLa cells were grown in antibiotic free medium, and siRNAs delivered with Lipofectamin RNAiMax. Cells were collected 72 hours post transfection. DMEM with 10% FBS at 37°C with 5% CO2 injection. Cells were fractionated into nuclear and cytoplasmic fractions with appropriate detergent conditions, and RNA was isolated with Trizol and RNA isolation columns from Zymogen. RNA was depleted of rRNA using the RiboZero kit (Epicentre) and converted to DNA libraries with the TruSeq stranded RNAseq library kit (Illumina).