title: MCV-miR-M1-5p mimic 2
organism: Homo sapiens
description: Protocols: 293 cells were transfected with either MCV-miR-M1-5p, MCV-miR-M1-3p or control mimic (Thermo Fisher Scientific). RNA extraction was carried out using Arum total RNA extraction kit (Bio-Rad) and confirmation of MCV miRNA 5p and 3p expression via stem loop qRT-PCR. Total RNA libraries were prepared using TruSeq Stranded Total RNA Sample Prep Kit (Illumina, USA).