title: RAX1-GR_mock_3
organism: Arabidopsis thaliana
description: Protocols: 14 day-old seedlings grown on MS plates were shortly immersed with a solution containing 0.03% L-77 Silwett, 1/10 000 DMSO (mock) or 10 µM DEX (DEX). Whole seedlings were harvested 4 h after treatment and immediately flash-frozen in liquid nitrogen. Seedlings were grown for 14 days of 1/2MS-Agar plates with 10% in long-days conditions. RNA was extracted with the RNAeasy kit (Qiagen) and DNA was removed with the TURBO DNA-free kit (Ambion) according to manufacturers' instructions. Libraries were synthesized with the TruSeq Stranded kit (Illumina) according to manufacturer's instructions. Library preparation was performed at the POPS plateform (IPS2, Paris-Saclay).