title: med20 kin28-AA plus rapa med15-myc rep1
organism: Saccharomyces cerevisiae
description: Protocols: Yeast were grown at 30˚C in YPD to A600 of 0.8; rapamycin added to 1 microgram/ml and incubated 1 hr additional ChIP performed by standard methods. Antibody incubation was overnight at 4 C. The NEB Next Ultra II DNA library prep kit was used according to manufacturer's instructions.