title: Adults
organism: Oikopleura dioica
description: Total RNA extracted and isolated with RNeasy Micro Kit (Qiagen, 74004) from O. dioica fully matured adults (4 days after hatching). The O. dioica individuals were collected from the on-doing laboratory culture deriving from the wild population in Ishikawa Harbor, Okinawa. Further processing for mRNA selection was performed with Oligo-d(T)25 Magnetic Beads (NEB, E7490). Adapters for the creation of DNA libraries for the Illumina platform were added per manufacturer's guidance (NEB, E7805) as were unique indexed oligonucleotides (NEB, E7600) to each of the 3 staged samples. The cDNA library was sequenced paired-end with a 300-cycle MiSeq Reagent Kit v2 (Illumina, MS-102-2002) loaded at approximately 12 pM. The RNA-Seq data was used to assemble transcriptome of the Okinawan O. dioica with Trinity software and to predict gene model in the I69-5 assembly.