title: BH 1
organism: Burkholderia cenocepacia
description: Protocols: 24hour old biofilms treated for 24hours in 96 well plate harvested by two cycles of vortexing (5min) and sonicating (5min)on ice, collected in falcon tubes 96 well plates, approximately 10^8 cells per well Starting from a fresh culture, 4hours adhesion rinsing of the biofilm 20hours maturation 24 hour treatment with - physiological saline (control) - baicalin hydrate (250M)in physiological saline - tobramycin (768g/ml) in physiological saline - baicalin hydrate (250M) + tobramycin (768g/ml) in physiological saline Ambion RiboPure Bacteria Kit Truseq stranded Total RNA library prep