title: Polychromatophilic erythroblasts from Mus musculus, Strain: STOCK Klf1<+>/Klf1<+> /Llp
organism: Mus musculus
description: Flow-sorted polychromatophilic erythroblasts: Single cell suspensions of adult (6-8 weeks) whole spleens in PBS containing 0.5% BSA were depleted of CD45+ cells using mouse CD45 MicroBeads (Miltenyi Biotec). Cells were sorted on a FACSAria II flow cytometer (BD Biosciences) directly into lysis buffer containing -mercaptoethanol (Sigma) following staining with CD45-FITC, Ter119-PE and CD44-APC (BD Biosciences) to obtain purified erythroid precursor populations as described in Liu J et al, Blood. 2016;127(15):1856-62. Epub 2016/02/24. doi: 10.1182/blood-2016-01-694331. PubMed PMID: 26903544; PMCID: PMC4832505