title: Sample 3
organism: Mus musculus
description: Protocols: Single-cell suspension of thymus was obtained as described (Salou et al., 2018), suspensions from 6-8 B6-MAITCast thymii were pooled prior to MR1 tetramer staining (staining performed 30 min at room temperature) followed by tetramer-based magnetic enrichment (Legoux and Moon, 2012). FACS sorting was performed on an Aria III cell sorter (BD). Total MR1 tetramer+ cells were flow-sorted into CO2-independent medium supplemented with 10% FCS. Sorted cells were centrifuged and counted, and 7,000 cells were loaded onto the Chromium 3' chip. Nucleic acid extraction was performed with the 3' v2 library kit and following the standard procedures from 10x genomics. Reverse transcription and library preparation were performed with the 3' v2 library kit and following the standard procedures from 10x genomics.