organism: Homo sapiens
description: Vero cells (ATCC, CCL-81) were maintained in DMEM (Gibco) and primary astrocytes were maintained in DMEM/F12 (Gibco). MAYV strain 4675 (ATCC 66, GenBank accession #MK070492.1). Viral stocks used here MAYV (7 x 107 PFU/ml), OROV  (2 x 106 PFU/ml)  was propagated by serial passages in Vero cells by routine methods using DMEM. Astrocyte infections were performed at MOI 1 (2 x 105 cells/well) for all four viruses in 12 well plate during 1 h at 37 C and 5% CO2 in medium without FBS.