title: Tu_squ_1
organism: Staphylococcus epidermidis
description: Protocols: 250 ml flasks containing 50 ml THB were inoculated with 2ml overnight diluted to OD600 0.5. Flasks were incubated at 37 C 250 RPM (linear) until bacteria had reached OD600 0.5. Squalene was added to treated samples to a final concentration of 1 %. Cells were Harvested after 20 minutes treatment and resuspended in RNA later Extraction protocol described in Moran et al, 2017. Basically, enzymatic digest of cells, extraction using RNeasy columns (Qiagen), DNase treatment and then cleanup using RNA Minelute (Qiagen) epicentre ScriptSeq v2 strand specific