organism: uncultured eukaryote
description: Samples were collected by Niskin bottles at 5 and 50m and then sequentially filtered over 10 um and 0.2 um filters. Filters were stored in etOH at -20C and DNA was extracted with the MoBio PowerWater DNA kit.
attributes.tag: ENA first public
attributes.value: 2018-02-11
attributes.tag: ENA last update
attributes.value: 2017-12-12
attributes.tag: External Id
attributes.value: SAMEA104423155
attributes.tag: INSDC center alias
attributes.value: OKINAWA INSTITUTE OF SCIENCE AND TECHNOLOGY
attributes.tag: INSDC center name
attributes.value: OKINAWA INSTITUTE OF SCIENCE AND TECHNOLOGY
attributes.tag: INSDC first public
attributes.value: 2018-02-11T17:02:52Z
attributes.tag: INSDC last update
attributes.value: 2017-12-12T09:17:26Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: 201603_NagoBay6
attributes.tag: collection date
attributes.value: 2016-03-15
attributes.tag: environment (biome)
attributes.value: Coastal
attributes.tag: environment (feature)
attributes.value: 50m, 1.75L, 1um filter
attributes.tag: environment (material)
attributes.value: water
attributes.tag: environmental sample
attributes.value: Yes
attributes.tag: geographic location (country and/or sea)
attributes.value: Japan
attributes.tag: geographic location (latitude)
attributes.value: 26.521688
attributes.tag: geographic location (longitude)
attributes.value: 127.861402
attributes.tag: pcr primers
attributes.value: Fwd: CCAGCASCYGCGGTAATTCC, Rev: ACTTTCGTTCTTGATYR
attributes.tag: sample name
attributes.value: 201603_NagoBay6
attributes.tag: target gene
attributes.value: 18S
attributes.tag: target subfragment
attributes.value: V4