organism: Mus musculus
description: Protocols: 4 week old mice were sacrificed and DBA-positive renal tubules were isolated as previously described (Outeda, P.et al. Kidney international, 92(5), 1130-1144 (2017). & Dafinger, C. et al. Experimental & molecular medicine, 50(6), 75 (2018).). Total RNA was isolated from the DBA enriched fraction by using RNA using phenol-chloroform extraction. All the RNA samples were further treated with DNAse to eliminate genomic DNA contamination as per manufacture's instruction (Quick RNA kit, Zymoresearch). For RNAseq, libraries were prepared using TruSeq stranded mRNA kit (Illumina) according to the provider's protocol.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-03-31T17:01:55Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-07-25T13:27:21Z
attributes.tag: External Id
attributes.value: SAMEA4803577
attributes.tag: INSDC center name
attributes.value: Uniklinik Koln, Klinik II fur Innere Medizin Nephrolab Cologne, CECAD
attributes.tag: INSDC first public
attributes.value: 2019-03-31T17:01:55Z
attributes.tag: INSDC last update
attributes.value: 2018-07-25T13:27:21Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7077:89101
attributes.tag: age
attributes.value: 4
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: kidney cortex tubule cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: proximal tubule
attributes.tag: sample name
attributes.value: E-MTAB-7077:89101
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: C57BL/6