organism: Homo sapiens
description: Protocols: The sorting strategy for each sample is indicated in the file name and available in the processed metadata files. hPSCs were differentiated in a xeno-free composition of SFD medium 14, BSA was substituted with human serum albumin, HSA, (Irvine-Scientific). Day 0 differentiation medium, containing 10 ng/ml BMP4 was added to the colonies prior cutting. Cut colonies were transferred to a bacteriological grade well to form embryoid bodies and cultured for two days. At day 2 media was changed and supplemented with 3 M CHIR (StemMacs). At day 3, embryoid bodies were collected and dissociated in Accutase (Gibco) to single cell solution, cells were plated on tissue culture grade wells in SFD medium supplemented with 5 ng/ml bFGF and 15 ng/ml VEGF (2x105 cells/well on 6 well plates or 1x105 cells/well on 12 well plate). At day 6 media was changed for final haematopoietic induction in SFD medium supplemented with 5 ng/ml bFGF, 15 ng/ml VEGF, 30 ng/ml IL3, 10 ng/ml IL6, 5 ng/ml IL11, 50 ng/ml SCF, 2 U/ml EPO, 30 ng/ml TPO, 10 ng/ml FLT3L and 25 ng/ml IGF1. From day 6 onward, cytokines were replaced every two days. According to 10x manufacturer's protocol. According to 10x manufacturer's protocol.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-07-06T17:04:12Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-07-03T16:12:53Z
attributes.tag: External Id
attributes.value: SAMEA7038785
attributes.tag: INSDC center name
attributes.value: UOE
attributes.tag: INSDC first public
attributes.value: 2020-07-06T17:04:12Z
attributes.tag: INSDC last update
attributes.value: 2020-07-03T16:12:53Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-9295:Day 13 CD31+ adherent cells CITE-seq
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: SFCi55
attributes.tag: cell type
attributes.value: hematopoietic cell
attributes.tag: common name
attributes.value: human
attributes.tag: immunophenotype
attributes.value: CD31+
attributes.tag: progenitor cell type
attributes.value: induced pluripotent stem cell
attributes.tag: sample name
attributes.value: E-MTAB-9295:Day 13 CD31+ adherent cells CITE-seq
attributes.tag: scientific_name
attributes.value: Homo sapiens