organism: Mus musculus
description: Protocols: The cells used are mouse embryonic stem cells. For RNAseq ESC lines were grown for 7-10d to obtain a healthy, fast growing culture. Then 3x105 cells were seeded to 6wells and cells were grown for 48h before isolating RNA using the AllPrep DNA/RNA kit (Qiagen). RNA was prepared from three independent cell seedings to obtain triplicate samples. DNA was removed using the TURBO DNAfree kit (Ambion) and RNA integrity was assessed on a bioanalyzer. 4µg RNA were used and ERCC Spike-ins were added to prepare sequencing libraries using the ScriptSeq Complete Gold Kit (Illumina). ScriptSeq Index Primers (Illumina) were used to multiplex libraries.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-05-01T04:02:49Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-10-29T12:36:12Z
attributes.tag: External Id
attributes.value: SAMEA5053634
attributes.tag: INSDC center name
attributes.value: Bioinformatics Researcher
attributes.tag: INSDC first public
attributes.value: 2019-05-01T04:02:49Z
attributes.tag: INSDC last update
attributes.value: 2018-10-29T12:36:12Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7343:ZFC4mut_1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: ES-E14TG2a
attributes.tag: cell type
attributes.value: embryonic stem cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: embryo
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: Sall4 ZFC4mut
attributes.tag: organism part
attributes.value: inner cell mass
attributes.tag: sample name
attributes.value: E-MTAB-7343:ZFC4mut_1
attributes.tag: scientific_name
attributes.value: Mus musculus