organism: Escherichia coli K-12
description: Protocols: A serial-passage evolution experiment was performed with the studied strains. Upon each new serial passage (seed), a time-series sample (2 mL culture) was collected and stored at -20 deg. C. according to detailed specifications in manuscript. Seed number thereby refers to the number of passages that the population has been through, each passage was taken after a 16 hour time point. Growth in M9 medium according to manuscript specifications. As described in the manuscript, eight parallel lineages (c1-4 and c6-9) of E coli equipped with a mevalonic acid production pathway were cultured by serial passing. Each new serial passage/seed, (2 % volume) was conducted following 16 hours of cultivation to avoid stationary-phase culture. Nucleic acids extracted by standard plasmid extraction kit (Macharey Nagel) Nextera XT v2 with two extra limited PCR cycles
attributes.tag: ENA first public
attributes.value: 2018-02-09
attributes.tag: ENA last update
attributes.value: 2018-01-16
attributes.tag: External Id
attributes.value: SAMEA104469864
attributes.tag: INSDC center alias
attributes.value: DTU
attributes.tag: INSDC center name
attributes.value: Technical University of Denmark
attributes.tag: INSDC first public
attributes.value: 2018-02-09T17:02:51Z
attributes.tag: INSDC last update
attributes.value: 2018-01-16T10:57:36Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6182:EVO5c1s13
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: genotype
attributes.value: pe1 (atoB, mvaS, mvaE, araC)
attributes.tag: growth condition
attributes.value: M9 medium, 30 degrees celsius
attributes.tag: individual
attributes.value: c1
attributes.tag: passage
attributes.value: 13
attributes.tag: sample name
attributes.value: E-MTAB-6182:EVO5c1s13