organism: Clostridium acetobutylicum
description: Protocols: Samples for total RNA extraction were collected from fermentation broth at acidogenic phase (8 h) and solventogenic phase (16 h).The samples were collected by centrifugation at 4oC and 8000 ×g for 10 min and frozen immediately in liquid nitrogen. Total RNA was isolated using a RNeasy Mini Kit (Qiagen, Germany) according to the manufacturer's instructions. The cDNA libraries construction and sequencing were performed by the Beijing Genomics Institute (BGI, Shenzhen, China).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-05-01T04:02:49Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-10-25T14:38:45Z
attributes.tag: External Id
attributes.value: SAMEA5046353
attributes.tag: INSDC center name
attributes.value: School of Life Science and Biotechnology, Dalian University of Technology
attributes.tag: INSDC first public
attributes.value: 2019-05-01T04:02:49Z
attributes.tag: INSDC last update
attributes.value: 2018-10-25T14:38:45Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7341:55025-cac0437KO-8 h
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: genotype
attributes.value: cac0437 homozygous knockout
attributes.tag: growth condition
attributes.value: fermentation broth
attributes.tag: sample name
attributes.value: E-MTAB-7341:55025-cac0437KO-8 h
attributes.tag: scientific_name
attributes.value: Clostridium acetobutylicum
attributes.tag: strain
attributes.value: ATCC 55025