organism: Mus musculus
description: Protocols: ChIPmentaion experiments were performed using ZIC3 (Abcam, ab222124) antibody in the Rex1GFPd2 mouse embryonic stem cell line (ESC; parental line; E14Tg2a) and d1- and d2-EpiLC differentiated states. Cells were grown in 12ng/ml bFGF, 20ng/ml activin A, 1% KOSR on 5ug/ml bovine plasma fibronectin-coated dishes. ChIPmentation samples and libraries were generated as described previously (Schmidl et al. 2015 https://doi.org/10.1038/nmeth.3542) ChIPmentation Nextera XT DNA library preparation kit
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-01-31T17:02:06Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-09-14T10:33:38Z
attributes.tag: External Id
attributes.value: SAMEA4896482
attributes.tag: INSDC center name
attributes.value: The University of Manchester
attributes.tag: INSDC first public
attributes.value: 2019-01-31T17:02:06Z
attributes.tag: INSDC last update
attributes.value: 2018-09-14T10:33:38Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7208:Sample 1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: ES-E14TG2a
attributes.tag: cell type
attributes.value: embryonic stem cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: Rex1GFPd2
attributes.tag: organism part
attributes.value: inner cell mass
attributes.tag: sample name
attributes.value: E-MTAB-7208:Sample 1
attributes.tag: scientific_name
attributes.value: Mus musculus