organism: Homo sapiens
attributes.tag: isolate
attributes.value: immortalized cell line
attributes.tag: age
attributes.value: 31
attributes.tag: biomaterial_provider
attributes.value: J. A. Steitz lab frozen stock
attributes.tag: sex
attributes.value: female
attributes.tag: tissue
attributes.value: Cervical cancer cell line
attributes.tag: cell_line
attributes.value: HeLa cell line
attributes.tag: cell_subtype
attributes.value: Pool of "green" Human Fireworks HeLa cell line (constructed by Dr. Alexandrov) transduced with the GeCKO sgRNA library
attributes.tag: phenotype
attributes.value: Inhibition of the nonsense-mediated decay pathway increases the red fluorescence signal produced by these cells due to increased expression of red fluorescent protein (tdTomato); in these cells, expression of the green fluorescent protein (EGFP) serves as an internal expression control.
attributes.tag: sample_type
attributes.value: FACS-enriched sgRNAs
attributes.tag: BioSampleModel
attributes.value: Human