organism: human metagenome
description: Pairs of swab samples were taken from healthy subjects under endoscopic guidance from each of the left and right middle meatuses. PCR amplification of the ITS2 genomic region (ITS3-ITS4) incorporated DNA from both sides into reactions for each subject.
attributes.tag: isolation_source
attributes.value: Homo sapiens (sinonasal)
attributes.tag: collection_date
attributes.value: 2017
attributes.tag: geo_loc_name
attributes.value: New Zealand: Auckland
attributes.tag: lat_lon
attributes.value: 36.85 S 174.76 E
attributes.tag: samp_collect_device
attributes.value: Swab
attributes.tag: samp_mat_process
attributes.value: Fixed in RNAlater and frozen at -20 degrees celcius until DNA extraction (Qiagen DNA/RNA AllPrep kit), and PCR amplification of the ITS2 genomic region (ITS3-ITS4).
attributes.tag: samp_size
attributes.value: Pairs of swabs for each of left and right middle meatus
attributes.tag: Unique_Sample_ID
attributes.value: Nasal3_ITS2
attributes.tag: Sample_info
attributes.value: Fungi
attributes.tag: BioSampleModel
attributes.value: Metagenome or environmental