organism: sheep gut metagenome
attributes.tag: host
attributes.value: sheep
attributes.tag: isolation_source
attributes.value: rumen
attributes.tag: collection_date
attributes.value: 2014-10-17
attributes.tag: geo_loc_name
attributes.value: USA: Bozeman, MT
attributes.tag: lat_lon
attributes.value: 45.6618 N 111.0735 W
attributes.tag: samp_collect_device
attributes.value: stomach tubing
attributes.tag: samp_mat_process
attributes.value: Rumen contents were sampled using a foal tube with a 600cc large animal dosing syringe, and stored at -20¡C until processing. Samples were thawed on ice, and both the solid and liquid portions of the rumen digesta were extracted for DNA via the MoBio PowerFecal DNA Isolation kit (Carlsbad, CA, USA), following manufacturer protocols. PCR amplification, amplicon quantification and purification, and Illumina MiSeq sequencing were performed as previously described (Ishaq et al., 2016).
attributes.tag: samp_size
attributes.value: 50 ml
attributes.tag: source_material_id
attributes.value: PelletedAlfalfa_wk0_29
attributes.tag: ID
attributes.value: PelletedAlfalfa_wk0_29
attributes.tag: Diet
attributes.value: PelletedAlfalfa
attributes.tag: Week
attributes.value: wk0
attributes.tag: Sheep_ID
attributes.value: c4029
attributes.tag: Weight_(kg)
attributes.value: 76
attributes.tag: Breed
attributes.value: Rambouillet wethers
attributes.tag: CPK
attributes.value: 349.0
attributes.tag: AST
attributes.value: 110.0
attributes.tag: FFT
attributes.value: 69.0
attributes.tag: ALKP
attributes.value: 172.0
attributes.tag: GLU
attributes.value: 88.0
attributes.tag: T. PRO
attributes.value: 6.8
attributes.tag: ALB
attributes.value: 3.4
attributes.tag: GLOB
attributes.value: 3.4
attributes.tag: CA
attributes.value: 10.8
attributes.tag: PHOS
attributes.value: 6.2
attributes.tag: BUN
attributes.value: 33.0
attributes.tag: CREAT
attributes.value: 0.8
attributes.tag: T. BILI
attributes.value: 0.3
attributes.tag: D. BILI
attributes.value: 0.0
attributes.tag: NA
attributes.value: 145.0
attributes.tag: K
attributes.value: 5.2
attributes.tag: CL
attributes.value: 104.0
attributes.tag: TC02
attributes.value: 21.8
attributes.tag: MG
attributes.value: 2.5
attributes.tag: HEMOLYSIS INDEX
attributes.value: 0.33
attributes.tag: ICTERIC INDEX
attributes.value: 0.17
attributes.tag: LIPEMIC INDEX
attributes.value: 0.17
attributes.tag: BioSampleModel
attributes.value: Metagenome or environmental