organism: Drosophila melanogaster
description: Protocols: The Decepentaplegic receptor Thickviens (Tkv) was over-expressed using the UASp-GAL4 system. pUASp-tkvQD was obtained from this original stock yw;pUASpTkvQD/TM6B (Casanueva and Ferguson, 2004; Development, 131(9): 1881-90). Gal4-Tub:VP16 was obtained from the original stock, Bloomington 7062. At the 3-3.5h time point after egg lay (AEL) embryos over-expressing TkvQD, derived from Gal4-Tub:VP16/+ ; P{UASp-TkvQD}/+ adults were collected. As an experimental control, 3-3.5h embryos derived from Gal4-Tub:VP16/+ ; + were also collected. RNA was extracted from 30ul of Drosophila embryos per sample using Trizol (Invitrogen) SOLiDTM Total RNA-Seq Kit (PN 4445374)
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2016-06-15T17:03:11Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T16:50:35Z
attributes.tag: External Id
attributes.value: SAMEA2225911
attributes.tag: INSDC center name
attributes.value: University of Manchester
attributes.tag: INSDC first public
attributes.value: 2016-06-15T17:03:11Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T16:50:35Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-1976:E1
attributes.tag: age
attributes.value: 3-3.5 hour
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: fruit fly
attributes.tag: developmental stage
attributes.value: larval stage
attributes.tag: genotype
attributes.value: Gal4-Tub:VP16/+ ; P{UASp-TkvQD}/+
attributes.tag: sample name
attributes.value: E-MTAB-1976:E1
attributes.tag: scientific_name
attributes.value: Drosophila melanogaster