organism: Homo sapiens
description: Protocols: SW480 cells were edited by CRISPR-CAS9 for the residue 400 to 411 (\NNGSLSAEFKHL\) of Stat3 deletion. 10% whole cell lysates were saved as input after genomic DNA was broken into 200-500 bp by sonication. 1 μg IP grade antibodies of STAT3 were incubated with the rest of the lysate overnight, followed by 2 h protein-A beads incubation at 37 °C for target protein pull down. The STAT3 enriched DNA or input DNA were repaired to 3'-dA overhang and added the ligated adapter. 0.2 ug of pull down DNA sample was submitted to NOVOGENE (Beijing, China) for library generation and sequencing.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-07-06T04:03:41Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-10-08T10:56:39Z
attributes.tag: External Id
attributes.value: SAMEA6059153
attributes.tag: INSDC center name
attributes.value: 3. Department of General Surgery, Shanghai Sixth People's Hospital East Affiliated to Shanghai University of Medicine & Health Sciences, Shanghai, 201308, China
attributes.tag: INSDC first public
attributes.value: 2020-07-06T04:03:41Z
attributes.tag: INSDC last update
attributes.value: 2019-10-08T10:56:39Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8417:mutant_1_stat3
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: SW480
attributes.tag: cell type
attributes.value: epithelial cell of large intestine
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: colorectal carcinoma
attributes.tag: genotype
attributes.value: STAT3 knockout
attributes.tag: organism part
attributes.value: colon
attributes.tag: sample name
attributes.value: E-MTAB-8417:mutant_1_stat3
attributes.tag: scientific_name
attributes.value: Homo sapiens