organism: Helianthus annuus
description: . Pieces of young leaves (around 250 mg per plant) were sampled from 12 plantlets (3 weeks old). The pool of leaves was grinded in liquid nitrogen using an electric grinder (Retsch ZM200) and stored at -80°C.
attributes.tag: Title
attributes.value: Genotypage SF296
attributes.tag: Genotype
attributes.value: SF296
attributes.tag: Molecule
attributes.value: genomic_DNA
attributes.tag: Extract_protocol
attributes.value: gDNA was extracted using the DNeasy Plant Maxi Kit (Qiagen) from 1g of leaves powder. gDNA was eluted in 1.5 ml of buffer AE.
attributes.tag: Growth_protocol
attributes.value: Plants were grown in a growth chamber. The controlled conditions were a 14h day (7:00 - 21:00) at 20°C and 75% RH and a 10h night (21:00 - 7:00) at 20°C and 75% RH.