organism: Mus musculus
description: Protocols: C57BL/6 wild type and multiple system atrophy transgenic mice (Tg(Plp1-SNCA)1Haa strain backcrossed in C57BL/6 in over 20 generations) at an age of 2-3 months were used in this study. The transgenic mice have a transgenic insertion of the human wild-type alpha synuclein driven by the proteolipid protein promoter. Transgenic protein is detected in oligodendrocytes but in no other brain cell type. The mice were sacrificed by cervical dislocation, brains were quickly extracted and the substantia nigra and striatum were dissected on ice. Three pools of substantia nigra and striatum, respectively, from 5 mice each were prepared and frozen in liquid nitrogen. Total RNA was isolated using Tri-Reagent (Sigma-Aldrich, St. Louis, Missouri, USA) according to the manufacturer's instructions and dissolved in DEPC-water. RNA was then submitted to polyA-enrichment (Dynabeads mRNA Purification Kit, Life technologies) using 6 g of total RNA from each pooled sample. With the polyA-enriched RNA as input, libraries were generated with the Ion Total RNA-Seq Kit v2 (Ion torrent, Life Technologies) and barcoded following the manufacturers instructions, except that the final bead-based size selection was adjusted to favor longer fragments (average read lengths >150 bp).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2016-03-15T17:02:24Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-09T09:23:37Z
attributes.tag: External Id
attributes.value: SAMEA3638992
attributes.tag: INSDC center name
attributes.value: Innsbruck medical University
attributes.tag: INSDC first public
attributes.value: 2016-03-15T17:02:24Z
attributes.tag: INSDC last update
attributes.value: 2018-03-09T09:23:37Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-3993:Sample 6 WT_ST
attributes.tag: biopsy site
attributes.value: striatum
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: wild type
attributes.tag: organism part
attributes.value: brain
attributes.tag: sample name
attributes.value: E-MTAB-3993:Sample 6 WT_ST
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: strain
attributes.value: C57BL/6