organism: Aphidius ervi
description: Adult parasitoids (males and females) were collected live and stored in 1.5 ml centrifuge tubes containing ethanol 95% at -20°C until DNA extraction
attributes.tag: strain
attributes.value: Sitobion avenae (SA)
attributes.tag: age
attributes.value: adult
attributes.tag: dev_stage
attributes.value: adult
attributes.tag: sex
attributes.value: pooled male and female
attributes.tag: tissue
attributes.value: whole body
attributes.tag: biomaterial_provider
attributes.value: Universidad de Talca, Talca, Chile
attributes.tag: breeding_history
attributes.value: Aphidius ervi parasitoids were reared in fresh host plants containing wasp-naïve aphids from the same aphid host from which they emerged under controlled conditions [16 h light: 8 h dark, 20°C]. Grain aphids were maintained on barley (Hordeum vulgare L.). These lineages were propagated for approximately 75 generations under controlled conditions . Each week, fresh host plants containing wasp-naïve aphids were introduced into the A. ervi rearing cages for parasitoid population maintenance, together with vials containing diluted honey and water for adult parasitoid feeding. Further reduction of intra-population genetic differences was accomplished by establishing isofemale parasitoid lines. These isofemale lines were maintained as described previously and propagated for approximately 10 generations before wasps' collection for genomic sequencing.
attributes.tag: collected_by
attributes.value: Gabriel Ballesteros
attributes.tag: isolation_source
attributes.value: These lineages were first collected as parasitized aphids (recognizable as mummies) from fields of wheat (Triticum aestivum L.) in Chile: Region de Los Rios.
attributes.tag: lat_lon
attributes.value: 39.51 S 73.7 W
attributes.tag: BioSampleModel
attributes.value: Model organism or animal