organism: Caenorhabditis elegans
description: The worm strain used was N2. This is a standard wild-type worm strain.  Embryos were obtained by bleaching synchronized gravid adults. The embryos were incubated for 18 hours at 20 degree Celsius in M9 buffer. The synchronized L1 worms were then fed with E. coli strain OP50 suspended in S-complete medium for 3 hours at 20 degree Celsius. The L1 worms were cleaned by washing with the M9 buffer and total homogenate was prepared by grinding the worms in liquid nitrogen. Nuclei were harvested by centrifuging the total homogenate. Nucleoplasmic fraction (clear supernatant) was prepared by treating the nuclei with Nuclear Lysis Buffer followed by centrifugation. Total RNAs were extracted from the nucleoplasmic fraction by using Sepasol I Super (nacalai tesque) and then purified by using RNeasy Plus Mini kit plus DNase I (Qiagen).
attributes.tag: sample_name
attributes.value: 12_N2_L1_Nucloplasm
attributes.tag: strain
attributes.value: N2
attributes.tag: biomaterial_provider
attributes.value: Hidehito KUROYANAGI
attributes.tag: collection_date
attributes.value: 2014-03-18
attributes.tag: dev_stage
attributes.value: L1
attributes.tag: genotype
attributes.value: wild type
attributes.tag: sex
attributes.value: hermaphrodite