organism: Homo sapiens
description: Protocols: VCF and VNCF were isolated from fetal human cardiac fibroblast by MACS against VCAM1. Fetal human cardiac fibroblasts were cultured in HFDM-1(+) medium supplemented with 1% (v/v) Newborn Calf Serum (NBCS). After initial expansion, fibroblasts were incubated with a biotin-conjugated anti-CD106 (VCAM1) antibody and anti-biotin microbeads for isolation of VCF and VNCF by MACS. Total RNA was extracted using the RNeasy Plus Mini Kit following the manufacturer's instructions (Qiagen). The library was constructed using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) by GENEWIZ.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-08-08T17:06:01Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-06-02T16:04:09Z
attributes.tag: External Id
attributes.value: SAMEA6870929
attributes.tag: INSDC center name
attributes.value: Metcela Inc.
attributes.tag: INSDC first public
attributes.value: 2020-08-08T17:06:01Z
attributes.tag: INSDC last update
attributes.value: 2020-06-02T16:04:09Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-9161:F2-3
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: fibroblast of cardiac tissue
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: embryo
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: heart
attributes.tag: phenotype
attributes.value: VCAM1 positive
attributes.tag: sample name
attributes.value: E-MTAB-9161:F2-3
attributes.tag: scientific_name
attributes.value: Homo sapiens