organism: Phytophthora cinnamomi
description: Protocols: Zoospores were collected from Phytophthora cinnamomi. The spores were vigorously shaken to induce incystment and allowed to germinate in distilled water at 19 degrees celsius for 90 minutes. Germinated cysts were harvested via centrifugation (10 000 rpm fro 5 minutes), frozen in liquid nitrogen and stored at -70 degrees Celsius. Fragmentation of mRNA enriched RNA (oligodT-primers) and random hexamer primed cDNA synthesis.
attributes.tag: ENA first public
attributes.value: 2016-06-15
attributes.tag: ENA last update
attributes.value: 2015-02-23
attributes.tag: External Id
attributes.value: SAMEA3267218
attributes.tag: INSDC center alias
attributes.value: Forestry and Agricultural Biotechnology Institute
attributes.tag: INSDC center name
attributes.value: Forestry and Agricultural Biotechnology Institute
attributes.tag: INSDC first public
attributes.value: 2016-06-15T17:03:14Z
attributes.tag: INSDC last update
attributes.value: 2015-02-23T14:31:29Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-3334:Phytophthora cinnamomi RNAseq
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: developmental stage
attributes.value: Germinating cysts
attributes.tag: sample name
attributes.value: E-MTAB-3334:Phytophthora cinnamomi RNAseq