organism: Acidiphilium sp. C61
description: Protocols: Acidiphilium sp. C61 was isolated by Mori,J.F. and Lu,S. Acidiphilium sp. C61 cultures were cultivated in APPW+YE+Glucose medium with 0 µM or 10 µM PEA. APPW+ Yeast extract+ Glucose Harvested bacterial cells were lysed by TNS solution and subjected to beadbeating. Cell debris was separated by centrifugation and the obtained supernatant was first extracted with phenol-chloroform-isoamyl alcohol and second with chloroform-isoamyl alcohol. The obtained aqueous phase was subjected to overnight precipitation at 4°C with two volumes of polyethylene glycol 6000 and 5 μl glycogen. The resulting pellets were washed with ice-cold ethanol and suspended in 50 µl elution buffer. DNA were removed through DNAase digestion from nucleic acid and RNA was purified through Zymo RNA clean kit. Purified total RNA were used to do cDNA libraries. The library was constructed based on NEBNext ultra directional RNA library prep kit. Fragmented and primed RNA was reverse transcripted to first strand cDNA and further synthesized to second strand cDNA. End prep of cDNA library and adaptor ligation were followed. Finallt, PCR library enrichment was conducted.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-03-01T04:04:39Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-12-18T16:47:50Z
attributes.tag: External Id
attributes.value: SAMEA6429261
attributes.tag: INSDC center name
attributes.value: Friedrich-Schiller-Universitat Jena
attributes.tag: INSDC first public
attributes.value: 2020-03-01T04:04:39Z
attributes.tag: INSDC last update
attributes.value: 2019-12-18T16:47:50Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8619:Sample 2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: growth condition
attributes.value: APPW+YE+Glucose medium
attributes.tag: organism part
attributes.value: whole organism
attributes.tag: sample name
attributes.value: E-MTAB-8619:Sample 2
attributes.tag: scientific_name
attributes.value: Acidiphilium sp. C61