organism: Homo sapiens
description: Protocols: Cells were maintained in 60mm dishes at 37 °C in a humidified atmosphere at 5% CO2 in DMEM 4.5g/L Glucose with UltraGlutamine media supplemented with 10% of Tet-free FBS (Clontech) and 1% penicillin/streptomycin. Cells were washed twice with PBS and added 700µL of Trizol and continue with the extraction protocol. Total RNA from HEK293 and HeLa was extracted using the miRNeasy Mini kit. For each sample, we obtain a yield of 20 µg of total RNA. 20 μg of 60-100 nt-sized total RNA were hydrolyzed during 10 min. was treated following the protocol of hydro-tRNAseq (Gogakos et al. 2017) cDNA library preparation protocol as described previously (Garzia et al. 2016).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-02-28T17:04:39Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-07-15T12:03:01Z
attributes.tag: External Id
attributes.value: SAMEA5774986
attributes.tag: INSDC center name
attributes.value: Centre for Genomic Regulation (CRG), The Barcelona Institute of Science and Technology, Dr. Aiguader 88, Barcelona 08003, Spain
attributes.tag: INSDC first public
attributes.value: 2020-02-28T17:04:39Z
attributes.tag: INSDC last update
attributes.value: 2019-07-15T12:03:01Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8144:LA3_31415_GTTTCG
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: HeLa
attributes.tag: cell type
attributes.value: epithelial cell
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: adenocarcinoma
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: uterine cervix
attributes.tag: sample name
attributes.value: E-MTAB-8144:LA3_31415_GTTTCG
attributes.tag: scientific_name
attributes.value: Homo sapiens