organism: Homo sapiens
description: Protocols: 100 (or less, if 100 were not available) highly purified Haematopoietic stem and progenitor cells (HSPCs) from cord blood samples were sorted directly into lysis buffer in RNAse inhibitor (Clontech) and stored at -80°C before further processing. cDNA synthesis was done with Smarter Ultra low input RNA kit v1 (Clontech). Illumina libraries were generated using Nextera XT DNA (Illumina). Library size and quality were checked using Agilent High-Sensitivity DNA chip with Agilent Bioanalyser (Agilent Technologies). Concentration of indexed libraries was determined using Qubit High-Sensitivity DNA kit (Invitrogen).
attributes.tag: ENA first public
attributes.value: 2017-12-06
attributes.tag: ENA last update
attributes.value: 2017-02-02
attributes.tag: External Id
attributes.value: SAMEA78568168
attributes.tag: INSDC center alias
attributes.value: UCL Institute of Child Health
attributes.tag: INSDC center name
attributes.value: UCL Institute of Child Health
attributes.tag: INSDC first public
attributes.value: 2017-12-06T17:02:30Z
attributes.tag: INSDC last update
attributes.value: 2017-02-02T15:22:54Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-5456:OX_PV_080
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: common myeloid progenitor
attributes.tag: common name
attributes.value: human
attributes.tag: individual
attributes.value: 151
attributes.tag: organism part
attributes.value: umbilical cord blood
attributes.tag: phenotype
attributes.value: Lin-CD34+CD38+CD123+CD45RA-CD10-
attributes.tag: sample name
attributes.value: E-MTAB-5456:OX_PV_080