organism: Homo sapiens
description: Protocols: Separate stromal and tumour fractions in 25cm2 flasks were washed with PBS, incubated in accutase for 5-10 mins, counted and resuspended at 750,000 cells/ml in OCMI without cholera toxin. Fluidigm Protocol P/N 100-7168 (Vers. I1) Using C1 to generate single-cell cDNA libraries for mRNA sequencing Illumina Nextera XT DNA library preparation guide P/N 15031942 (Rev. c) modified according to Fluidigm Protocol P/N 100-7168 (Vers. I1)
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-04-30T04:02:44Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-10-04T10:41:52Z
attributes.tag: External Id
attributes.value: SAMEA4952404
attributes.tag: INSDC center name
attributes.value: The University of Manchester
attributes.tag: INSDC first public
attributes.value: 2019-04-30T04:02:44Z
attributes.tag: INSDC last update
attributes.value: 2018-10-04T10:41:52Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7249:Sample 139
attributes.tag: age
attributes.value: 81
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: epithelial cell
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: ovarian serous adenocarcinoma
attributes.tag: disease staging
attributes.value: stage 3C
attributes.tag: individual
attributes.value: OCM.38
attributes.tag: organism part
attributes.value: ascitic fluid
attributes.tag: sample name
attributes.value: E-MTAB-7249:Sample 139
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: female
attributes.tag: tumor grading
attributes.value: grade 3