organism: Homo sapiens
description: Protocols: LNCAP cells were seeded at an appropriated density and dosed 24 hours later with 1uM of each compound. Either 24 or 48 hours later cells were lysed in RLT buffer (Qiagen) on ice; adherent cells were scraped and the material was passed through a QIAshredder (Qiagen). Samples were then snap frozen on dry ice and stored at -80ºC. Samples were randomised and extracted on Qiacube-HT following the RNeasy 96 Qiacube HT total RNA Cell with DNase protocol. RNA concentration and quality was assessed with Qubit RNA BR kit (Invitrogen) and Agilent RNA 6000 Nano kit. Libraries were prepared using the TruSeq Stranded mRNA library preparation kit as per the manufacturers guidelines
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-03-31T17:01:57Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-10-12T09:25:52Z
attributes.tag: External Id
attributes.value: SAMEA4971983
attributes.tag: INSDC center name
attributes.value: AZ
attributes.tag: INSDC first public
attributes.value: 2019-03-31T17:01:57Z
attributes.tag: INSDC last update
attributes.value: 2018-10-12T09:25:52Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7294:Sample 15
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: LNCAP
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: prostate carcinoma
attributes.tag: organism part
attributes.value: prostate
attributes.tag: sample name
attributes.value: E-MTAB-7294:Sample 15
attributes.tag: scientific_name
attributes.value: Homo sapiens