organism: Homo sapiens
description: Protocols: Primary human dermal microvascular blood and lymphatic endothelial cell lines were fixed using 1% formaldehyde and used for ChIP sequencing. Chromatin was prepared using the iDeal ChIP-Seq kit for Histones and sheared using Bioruptor Pico sonication device combined with the Bioruptor Water cooler for 12 cycles using a 30'' [ON] 30'' [OFF] settings. ChIP was performed using IP-Star Compact Automated System and antibodies against H3K27me3 and H3K4me3; chromatin (1% ) was set as Input. Libraries were prepared from input and immunoprecipitated DNA using the MicroPlex Library Preparation Kit. After library amplification, libraries were then purified using Agencourt AMPure XP (Beckman Coulter) and quantified using Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2021-01-18T04:13:33Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-04-16T11:36:09Z
attributes.tag: External Id
attributes.value: SAMEA6786250
attributes.tag: INSDC center name
attributes.value: PhD student, Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zurich
attributes.tag: INSDC first public
attributes.value: 2021-01-18T04:13:33Z
attributes.tag: INSDC last update
attributes.value: 2020-04-16T11:36:09Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8951:Sample 6
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: blood vessel endothelial cell
attributes.tag: cell type
attributes.value: blood vessel endothelial cell
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: neonate
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: foreskin
attributes.tag: replicate
attributes.value: 2
attributes.tag: sample name
attributes.value: E-MTAB-8951:Sample 6
attributes.tag: scientific_name
attributes.value: Homo sapiens