organism: Homo sapiens
description: Protocols: 7 million cells for each of three biological replicates were collected off plates using trypsin and stored in TRIzol at -80 C until RNA extraction. Total RNA was extracted from TRIzol (Invitrogen) following manufacturer protocol. Any DNA contamination was removed from the extracted total RNA using the RNase Free DNase (Promega) followed by column clean-up with RNeasy Mini Kit (Qiagen). Depletion of rRNA and RNA-seq libraries were prepared from 1ug of total RNA using the SMARTer Stranded Total RNA Sample Prep Kit (Takara Bio USA, Inc.) following standard protocols.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-04-30T04:02:48Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-02-13T15:38:58Z
attributes.tag: External Id
attributes.value: SAMEA5336115
attributes.tag: INSDC center name
attributes.value: The Children's Hospital of Philadelphia
attributes.tag: INSDC first public
attributes.value: 2019-04-30T04:02:48Z
attributes.tag: INSDC last update
attributes.value: 2019-02-13T15:38:58Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7676:Sample 1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: EndoC-betaH1
attributes.tag: common name
attributes.value: human
attributes.tag: replicate
attributes.value: 1
attributes.tag: sample name
attributes.value: E-MTAB-7676:Sample 1
attributes.tag: scientific_name
attributes.value: Homo sapiens