organism: Homo sapiens
description: Protocols: In accordance with the study protocol approved by institutional review boards of Universitair Ziekenhuis Brussel and Antwerp University Hospital, NSCLC patients with history of non-smoking (strictly never smoked or smoked less than 100 cigarettes in their life time) or former limited smoking (smoked less than five packed years with a smoking free interval of more than 20 years) were enrolled from 2013 to 2015 for the present study, which is called the Belgian FIELT-2 study. The majority of these patients had stage IV disease. Genomic DNA extraction from tumour material and matched normal tissue were performed with the the QIAamp DNA FFPE Tissue Kit and the QIAamp DNA Blood Midi/Maxi Kit (Qiagen Inc., Valencia, CA) respectively, according to the manufacturer's instructions. Quality and quantity of purified DNA was accessed by the NanoDrop (Fisher Thermo, Wilmington, DE) Whole-genome DNA libraries were created with the Illumina TruSeq sample preparation kit V2 according to manufacturer's instructions.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-08-07T04:04:06Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-03-22T15:39:33Z
attributes.tag: External Id
attributes.value: SAMEA5532535
attributes.tag: INSDC center name
attributes.value: VIB Center for Cancer Biology (CCB) Laboratory for Translational Genetics Department of Human Genetics KULeuven
attributes.tag: INSDC first public
attributes.value: 2020-08-07T04:04:06Z
attributes.tag: INSDC last update
attributes.value: 2019-03-22T15:39:33Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7811:sFLT051_T1
attributes.tag: age
attributes.value: 63
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: clinical history
attributes.value: strict never-smoker
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: lung adenocarcinoma
attributes.tag: disease staging
attributes.value: IV
attributes.tag: individual
attributes.value: 51
attributes.tag: organism part
attributes.value: lung
attributes.tag: sample name
attributes.value: E-MTAB-7811:sFLT051_T1
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: female