organism: Homo sapiens
description: Protocols: Monocytes were isolated from the peripheral blood of healthy donors by Ficoll-Paque density centrifugation followed by immunomagnetic selection using CD14+ microbeads (MACS monocyte isolation kit, Miltenyi Biotec) according to manufacturer's instructions. MDMs were treated with anti-CCL2 or control Ab for 4 h and then infected with 3,000 tissue culture infectious dose (TCID50) per well of the CCR5-dependent HIV-1BaL strain pelleted virus (Advanced Biotechnologies), corresponding to a MOI ≈ 0.03. After 2 h, cells were washed and maintained in complete medium either in the presence or in the absence of anti-CCL2 or control Ab Total RNA samples were isolated with the RNeasy Mini kit (Qiagen) following the manufacturer's instructions. RNA concentration and integrity were analyzed by a NanoDrop 2000 (Thermo Scientific) and a TapeStation (Agilent). Total RNA was subjected to poly (A) selection followed by reverse transcription
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-08-06T04:09:32Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-08-05T18:18:29Z
attributes.tag: External Id
attributes.value: SAMEA7129784
attributes.tag: INSDC center name
attributes.value: UCLA Institute for Quantitative and Computational Biosciences, University of California at Los Angeles, Los Angeles, CA, USA
attributes.tag: INSDC first public
attributes.value: 2020-08-06T04:09:32Z
attributes.tag: INSDC last update
attributes.value: 2020-08-05T18:18:29Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-9414:s12
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: monocyte derived macrophage
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: normal
attributes.tag: individual
attributes.value: donor4
attributes.tag: organism part
attributes.value: blood
attributes.tag: sample name
attributes.value: E-MTAB-9414:s12
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: stimulus
attributes.value: ControlAb