organism: Gallus gallus
description: Protocols: Pectoralis major muscle samples were collected from 35 days old chickens. Sampling (approx. 800 mg) was performed in the middle of the tissue. Samples were snap frozen in liquid nitrogen. standard Cobb500 growth condition. TM: 39.5°C and 65% RH 12h/days from embryonic days E7 to E16 and the rest of the time 37,8°C 55% RH during embryogenesis. no treatment (Control): 37,8°C and 55% RH during embryogenesis. Muscle native chromatin immunoprecipitation (ChIP) was performed as described in David et al., 2017 (doi: 10.1186/s12575-017-0059-0). Antibodies batches used: Millipore 07-449#2506493 (H3K27me3); Millipore 07-473#2717639 (H3K4me3). Sequencing libraries were prepared using the NEB Next Ultra II DNA Library Prep kit for Illumina (New England Biolabs, Ipswich, USA). Ten ng of H3K27me3 IP DNA, 10 ng of H3K4me3 IP DNA and 100 ng of input DNA were used. Agencourt AMPure XP beads (Beckman Coulter, Brea, USA) were used for the 200 bp size selection of DNA fragments. Libraries concentrations were measured by Qubit and stored at −80 °C until sequencing.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-01-31T17:02:07Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-10-16T14:23:41Z
attributes.tag: External Id
attributes.value: SAMEA4976024
attributes.tag: INSDC center name
attributes.value: BOA, INRA, Universite de Tours
attributes.tag: INSDC first public
attributes.value: 2019-01-31T17:02:07Z
attributes.tag: INSDC last update
attributes.value: 2018-10-16T14:23:41Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7297:TM8688_H3K4me3_VTCM103
attributes.tag: age
attributes.value: 35
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: chicken
attributes.tag: growth condition
attributes.value: temperature manipulation during embryogenesis
attributes.tag: organism part
attributes.value: pectoral muscle
attributes.tag: sample name
attributes.value: E-MTAB-7297:TM8688_H3K4me3_VTCM103
attributes.tag: scientific_name
attributes.value: Gallus gallus
attributes.tag: strain
attributes.value: Cobb500