organism: Drosophila melanogaster
description: Protocols: Embryos were collected from yw adults as in Saunders et al. 2013. Saunders et al. Extensive polymerase pausing during Drosophila axes patterning enables high-level and pliable transcription. 2013. Genes and Development. Published online May 15, 2013. http://dx.doi.org/10.1101/gad. 215459.113. Embryo ChIP was performed as described in Sandmann et al. 2006, except that 5% formaldehyde was used in the crosslinking solution. For PI samples, the guinea pig pre-immune serum was used instead of Brk antibody. Samples were sonicated for 30 min using a Bioruptor with 9 sec on, 1 min off cycles, on high-energy settings. Two independent biological replicates were performed for each ChIP at each time point. Sandmann T, Jakobsen JS, Furlong EE. ChIP-on-chip protocol for genome-wide analysis of transcription factor binding in Drosophila melanogaster embryos. 2006. Nature Protocols 1:2839-2855.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2016-06-15T17:03:10Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T16:27:21Z
attributes.tag: External Id
attributes.value: SAMEA2072672
attributes.tag: INSDC center name
attributes.value: University of Manchester
attributes.tag: INSDC first public
attributes.value: 2016-06-15T17:03:10Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T16:27:21Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-1673:Brk 3 to 3.5 hr IP exp1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: fruit fly
attributes.tag: developmental stage
attributes.value: embryo stage
attributes.tag: gestational age
attributes.value: 3 to 3.5 hour
attributes.tag: sample name
attributes.value: E-MTAB-1673:Brk 3 to 3.5 hr IP exp1
attributes.tag: scientific_name
attributes.value: Drosophila melanogaster
attributes.tag: sex
attributes.value: mixed sex