title: ROMEO2015.030.DNA_2015-09-21_Yuk_C_summer_river_water
organism: freshwater metagenome
description: USGS 15565447 Yukon River at Pilot Station, AK
attributes.tag: isolation_source
attributes.value: river water
attributes.tag: collection_date
attributes.value: 2015-09-21
attributes.tag: geo_loc_name
attributes.value: USA: Yukon River at Pilot Station, AK
attributes.tag: lat_lon
attributes.value: 61.933690 N 162.882940 W
attributes.tag: samp_collect_device
attributes.value: Collected into a 10L carboy dipped below the surface near the center of the river channel
attributes.tag: samp_mat_process
attributes.value: Material pre-filtered with a 3.0 micron pore-size, 142mm diameter polycarbonate filter, and collected on a 0.22 micron pore-size Sterivex filter (Millipore, Burlington, MA). Genomic DNA from Thermus thermophilus HB8 was added (44 ng) prior extraction for quantification analyses, as per Satinsky et al. 2013 (https://doi.org/10.1016/B978-0-12-407863-5.00012-5). DNA was extracted using MasterPure™ Complete DNA and RNA Purification Kit (Lucigen, Middleton, WI).
attributes.tag: samp_size
attributes.value: 1.3 L
attributes.tag: isolate
attributes.value: Yuk_C_summer
attributes.tag: replicate
attributes.value: replicate C
attributes.tag: BioSampleModel
attributes.value: Metagenome or environmental