title: Sample 8
organism: Mus musculus
description: Protocols: Mouse ureters were extracted from embryonic stage 15 mice and then cultured in serum-free defined media according to an established protocol (PMID: 24260555 and PMID: 18776146). Mouse experiments were approved by the University of Manchester ethics committee and the UK Home Office (licence PAFFCI44F). Mouse ureters were extracted from embryonic stage 15 mice and then cultured in serum-free defined media according to an established protocol (PMID: 24260555 and PMID: 18776146). There were 4 treatment conditions. Controls were grown in basal media or in the presence of 5 nanogram per milliliter recombinant human transforming growth factor-beta (TGFB1) for 1 day or 6 days. Each n=1 in the RNA-sequencing analyses comprised RNA extracted from the a set of six to eight ureters from the same litter grown in one condition for one or six days. Comparisons (n=3 for each experimental set) were made with paired ureter sets derived from the same embryonic litters. Mouse experiments were approved by the University of Manchester ethics committee and the UK Home Office (licence PAFFCI44F). Organs were collected in RNA protect (Thermo Fisher Scientific, Runcorn, UK) and RNA extracted using the RNeasy Plus Mini Kit (Qiagen). Truseq stranded mRNA Assay
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-08-07T17:03:22Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-11-13T17:15:39Z
attributes.tag: External Id
attributes.value: SAMEA5085235
attributes.tag: INSDC center name
attributes.value: UNIVERSITY OF MANCHESTER
attributes.tag: INSDC first public
attributes.value: 2019-08-07T17:03:22Z
attributes.tag: INSDC last update
attributes.value: 2018-11-13T17:15:39Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7395:Sample 8
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: embryonic day 15.0
attributes.tag: organism part
attributes.value: ureter
attributes.tag: sample name
attributes.value: E-MTAB-7395:Sample 8
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: strain
attributes.value: CD1 mus strain