title: DSMS2RD07
organism: soil metagenome
attributes.tag: Agricultural_practice
attributes.value: direct seeding with a Fitarelli jab planter with rotation of maize/sunn hemp
attributes.tag: Treatment
attributes.value: maize residue amendment
attributes.tag: Day_incubation
attributes.value: 7
attributes.tag: Replicate
attributes.value: 2
attributes.tag: collection_date
attributes.value: 2012-11
attributes.tag: depth
attributes.value: 0-15 cm
attributes.tag: alt_elev
attributes.value: 1136
attributes.tag: elevation
attributes.value: 1136
attributes.tag: env_biome
attributes.value: soil
attributes.tag: env_feature
attributes.value: Microcoms experiment
attributes.tag: env_material
attributes.value: soil
attributes.tag: geo_loc_name
attributes.value: Zimbabwe: Mashonaland Central, Mazowe
attributes.tag: lat_lon
attributes.value: 17.5725 S 30.987222 E
attributes.tag: ph
attributes.value: 5
attributes.tag: ph_meth
attributes.value: Potentiometric
attributes.tag: soil_type
attributes.value: Arensols and Luvisols
attributes.tag: soil_type_meth
attributes.value: FAO 1998
attributes.tag: texture
attributes.value: Loamy sand
attributes.tag: texture_meth
attributes.value: Bouyoucos hydrometer method
attributes.tag: tot_org_carb
attributes.value: 4.8 g/kg
attributes.tag: tot_org_c_meth
attributes.value: TOC analysis Shimadzu
attributes.tag: water_holding_capacity
attributes.value: 260
attributes.tag: WHC_meth
attributes.value: Gravimetric
attributes.tag: pcr_primers
attributes.value: FWD:AGAGTTTGATCNTGGCTCA REV:CCGTCWATTKCTTTGAGTT
attributes.tag: mid
attributes.value: TCGTCGCTCG
attributes.tag: adapters
attributes.value: CCATCTCATCCCTGCGTGTCTCCGACTCAG, CCTATCCCCTGTGTGCCTTGGCAGTCTCAG
attributes.tag: pcr_cond
attributes.value: initial denaturation:94_1; annealing:53_0.8; elongation: 72_1; final elongation:72_5; 25
attributes.tag: seq_meth
attributes.value: 454 titanium
attributes.tag: BioSampleModel
attributes.value: MIMS.me
attributes.tag: BioSampleModel
attributes.value: MIGS/MIMS/MIMARKS.soil