title: ROMEO2015.036.RNA_2015-10-21_Atlam_C_fall_river_water
organism: freshwater metagenome
description: USGS 02226160 Altamaha River at Everett City, GA
attributes.tag: isolation_source
attributes.value: river water
attributes.tag: collection_date
attributes.value: 2015-10-21
attributes.tag: geo_loc_name
attributes.value: USA: Altamaha River at Everett City, GA
attributes.tag: lat_lon
attributes.value: 31.427170 N 81.605390 W
attributes.tag: samp_collect_device
attributes.value: Collected into a 10L carboy dipped below the surface from a pier on the right bank of the river channel
attributes.tag: samp_mat_process
attributes.value: Material pre-filtered with a 3.0 micron pore-size, 142mm diameter polycarbonate filter, and collected on a 0.22 micron pore-size Sterivex filter (Millipore, Burlington, MA). Artificial transcript sequences (https://www.addgene.org; products MTST5 and MTST6) were added (0.65 ng of each) prior to nucleic acid extraction for quantification analyses, as per Satinsky et al. 2013 (https://doi.org/10.1016/B978-0-12-407863-5.00012-5). Following RNA extraction using the MasterPure™ Complete DNA and RNA Purification Kit (Lucigen, Middleton, WI), mRNA was enriched using Ribo-Zero microbial rRNA removal kit (Illumina, Inc.)
attributes.tag: samp_size
attributes.value: 1 L
attributes.tag: isolate
attributes.value: Atlam_C_fall
attributes.tag: replicate
attributes.value: replicate C
attributes.tag: BioSampleModel
attributes.value: Metagenome or environmental