title: si-p60_A
organism: Mus musculus
description: Protocols: Total RNA was extracted with GenElute total RNA miniprep kit, treated with turbo DNase and purified with RNeasy MinElute Cleanup kit. The quality was assessed on a bioanalyzer. ES cells were cultured in DMEM with GlutaMax (Life technologies) containing 15% FCS and LIF Libraries were prepared with Truseq Stranded Total RNA with Ribo-Zero Gold Prep kit (Illumina). Lipofectamine RNAi MAX (Life technologies) were used to transfect siRNA.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2015-08-02T17:01:19Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T19:31:17Z
attributes.tag: External Id
attributes.value: SAMEA2697773
attributes.tag: INSDC center name
attributes.value: Max Plank Institute for Molecular Biomedicine
attributes.tag: INSDC first public
attributes.value: 2015-08-02T17:01:19Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T19:31:17Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-2684:si-p60_A
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: ES-E14 cell
attributes.tag: cell type
attributes.value: embryonic stem cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: sample name
attributes.value: E-MTAB-2684:si-p60_A
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: strain
attributes.value: 129/Ola