title: cell_culture_Hypoxia_RNA-Pol2
organism: Homo sapiens
description: Protocols: MCF-7 cells were cultured in Dulbeccos modified Eagle's medium 'DMEM' (Invitrogen). Media were supplemented with and 10% Fetal Bovine Serum (FBS) (Sigma-Aldrich). Cells were incubated for 24 hours in an In vivo2 Hypoxia Work Station (Ruskin Technologies) in an atmosphere of either normoxia (21% oxygen) or hypoxia (0.5% oxygen). Chromatin immunoprecipitation was performed as described in the paper. Libraries were prepared using the Illumina ChIP-Seq kit.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2014-02-10T17:00:48Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T16:52:56Z
attributes.tag: External Id
attributes.value: SAMEA2242754
attributes.tag: INSDC center name
attributes.value: The Nuffield Department of Medicine, University of Oxford, Oxford, UK
attributes.tag: INSDC first public
attributes.value: 2014-02-10T17:00:48Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T16:52:56Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-1995:cell_culture_Hypoxia_RNA-Pol2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: MCF-7
attributes.tag: common name
attributes.value: human
attributes.tag: sample name
attributes.value: E-MTAB-1995:cell_culture_Hypoxia_RNA-Pol2
attributes.tag: scientific_name
attributes.value: Homo sapiens