title: De-etiolating seedling from Gynandropsis gynandra
organism: Gynandropsis gynandra
description: Gynandropsis gynandra seeds were sown directly from intact pods and germinated on moist filter papers in the dark at 32C for 24 hours. Germinated seeds were then transferred to half strength Murashige and Skoog (MS) medium with 0.8% (w/v) agar (pH 5.8) and grown for three days in a growth chamber at 26C. De-etiolation was induced by exposure to white light with a photon flux density (PFD) of 350 umol m-2 s -1 and photoperiod of 16 hours. Whole seedlings were harvested at 0.5, 2, 4 and 24 hours after illumination (starting at 8:00 with light cycle 6:00 to 22:00). Tissue was flash frozen in liquid nitrogen and stored at -80C prior to processing.
attributes.tag: ecotype
attributes.value: Malaysia-1
attributes.tag: age
attributes.value: 3 days
attributes.tag: dev_stage
attributes.value: De-etiolating seedling
attributes.tag: geo_loc_name
attributes.value: Malaysia
attributes.tag: tissue
attributes.value: Whole Seedling
attributes.tag: BioSampleModel
attributes.value: Plant