title: anoikis-resistant CAL27 cell sample1
organism: Homo sapiens
description: Anoikis resistance
attributes.tag: isolate
attributes.value: not collected
attributes.tag: age
attributes.value: not collected
attributes.tag: biomaterial_provider
attributes.value: Jun Jia and Rong Jia
attributes.tag: sex
attributes.value: not collected
attributes.tag: tissue
attributes.value: Oral cancer tissue
attributes.tag: cell_line
attributes.value: CAL27
attributes.tag: cell_type
attributes.value: cancer cell
attributes.tag: disease
attributes.value: oral cancer
attributes.tag: sample_type
attributes.value: cell culture
attributes.tag: treatment
attributes.value: To collecting anoikis-resistant CAL27 cells, according to Michael et al. previously reported procedures[15], approximately 2108 CAL27 cells were digested by 0.25% trypsin with 0.1% EDTA solution from monolayer growing in tissue culture flask. Then, the CAL27 cells were transferred to 10% poly-HEMA (Sigma Chemical Co. USA) coated tissue culture dish (10cm) for 72h in an incubator with moist air containing 5% CO2 at 37 C. Subsequently, these CAL27 spheroids cells were collected at 300 rpm for 5 min. Then, they were transferred to uncoated dish, and allowed to replicate for 24 h. After six rounds of coated and un-coated culture, anoikis-resistant CAL27 cells were harvested at 300 rpm for 5 min.
attributes.tag: replicate
attributes.value: biological replicate 1
attributes.tag: BioSampleModel
attributes.value: Human