title: Splenic B cells from wild-type C57BL/6J mice
organism: Mus musculus
description: Wild-type C57BL 6J mice were purchased from Oriental Yeast Co., ltd., (Tokyo, Japan) and were maintained under specific pathogen-free conditions. Mice 8-12 weeks of age were used in the study. All mice experiments were conducted in accordance with guidelines approved by the RIKEN Animal Committee. Splenic B cells were purified by depleting CD43+ cells with magnetic beads using AutoMACS (Miltenyi Biotec, Bergisch-Gladbach, Germany), and were cultured in Iscoves Modified Dulbeccos Medium supplemented with 10% FBS and 1% penicillin-streptomycin. Purified B cells were stimulated with 0, 0.01, 0.1, 1 and 10 ug/ml anti-mouse IgM mAb obtained from Jackson Immuno Research (West Grove, PA) for 1 hour.
attributes.tag: strain
attributes.value: C57BL/6J
attributes.tag: age
attributes.value: 8-12 weeks
attributes.tag: sex
attributes.value: pooled male and female
attributes.tag: tissue
attributes.value: Spleen
attributes.tag: cell_type
attributes.value: Splenic B cell
attributes.tag: genotype
attributes.value: Wild-type
attributes.tag: BioSampleModel
attributes.value: Model organism or animal