title: R1_15mM_24h
organism: Arabidopsis thaliana
description: Protocols: 3 hours and 24 hours after transfer, seedlings were harvested in liquid nitrogen, put in RNAlater ice solution and incubated at -20°C for at least one week, after which the third leaf was micro-dissected under a binocular light microscope. Seedlings are grown on a mesh covering MS media without carbon source, and subsequently transferred at 9 DAS to control media without sucrose or medium supplemented with 15 mM sucrose. Transfer at 9 DAS to control media without sucrose or medium supplemented with 15 mM sucrose Leaves were frozen in liquid nitrogen and RNA was extracted using Trizol (Invitrogen) and the RNeasy Plant Mini Kit (Qiagen). Library preparation was done using the TruSeq RNA Sample Preparation Kit v2 (Illumina). Briefly, polyA-containing mRNA molecules were reverse transcribed, double-stranded cDNA was generated and adapters were ligated. After quality control using 2100 Bioanalyzer (Agilent), clusters were generated through amplification using the TruSeq PE Cluster Kit v3-cBot-HS kit (Illumina).
attributes.tag: ENA first public
attributes.value: 2017-12-14
attributes.tag: ENA last update
attributes.value: 2016-11-17
attributes.tag: External Id
attributes.value: SAMEA4553569
attributes.tag: INSDC center alias
attributes.value: Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, Belgium Department of Plant Systems Biology,VIB, Gent, Belgium
attributes.tag: INSDC center name
attributes.value: Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, Belgium Department of Plant Systems Biology,VIB, Gent, Belgium
attributes.tag: INSDC first public
attributes.value: 2017-12-14T17:03:01Z
attributes.tag: INSDC last update
attributes.value: 2016-11-17T14:57:45Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-4262_2:R1_15mM_24h
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: thale cress
attributes.tag: ecotype
attributes.value: Col-0
attributes.tag: organism part
attributes.value: Leaf 3
attributes.tag: sample name
attributes.value: E-MTAB-4262_2:R1_15mM_24h
attributes.tag: time
attributes.value: 24