title: Col-0_T2
organism: Arabidopsis thaliana
description: Protocols: For RNA sequencing analysis, seedlings (12 DAS for Col-0 and Ey15-2 and 13 DAS for WalhaesB4, ICE97, ICE138, ICE75, Ler-0, Yeg-1, Sha, ICE153) were harvested in RNA ice-later solution (AM7030; Ambion) and incubated at -20°C for at least a week. Leaf 6 was micro-dissected on a cold plate with dry ice under a stereomicroscope, and frozen in liquid nitrogen. RNA was extracted with Trizol (Invitrogen) according to the manufacturer's instructions and the RNeasy kit (Qiagen) with on-column DNase (Qiagen) digestion. RNA was quantified and the quality was checked with a 2100 Bioanalyzer (Agilent). Library preparation was done using the TruSeq RNA Sample Preparation Kit v2 (Illumina). In brief, polyA containing mRNA molecules are reverse transcribed, double-stranded cDNA is generated and adapters are ligated.
attributes.tag: ENA first public
attributes.value: 2017-12-14
attributes.tag: ENA last update
attributes.value: 2016-02-29
attributes.tag: External Id
attributes.value: SAMEA3882030
attributes.tag: INSDC center alias
attributes.value: Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, Belgium Department of Plant Systems Biology,VIB, Gent, Belgium
attributes.tag: INSDC center name
attributes.value: Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, Belgium Department of Plant Systems Biology,VIB, Gent, Belgium
attributes.tag: INSDC first public
attributes.value: 2017-12-14T17:02:56Z
attributes.tag: INSDC last update
attributes.value: 2016-02-29T14:27:20Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-4508:Col-0_T2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: thale cress
attributes.tag: ecotype
attributes.value: Col-0
attributes.tag: genetic modification
attributes.value: GA20ox1 OE
attributes.tag: organism part
attributes.value: Leaf 6
attributes.tag: sample name
attributes.value: E-MTAB-4508:Col-0_T2