title: 09_08_S46
organism: Homo sapiens
description: Protocols: Human CD4+ T cells were isolated from PBMC by EasySep positive selection kit (Stemcell Technologies) according to the manufacturer's instructions. Positively selected CD4+ T cells were stained for subsequent TH cell subset sorting. Total RNA was isolated from TH1, TH2, TH17, and TH9 clones in the resting state with RNAready-kit (AmpTec) according to manufacturer's instruction. Peripheral Blood Mononuclear Cells (PBMC) were isolated by Ficoll-Plaque Plus (GE Healthcare, UK) density gradient centrifugation. Culture medium consisted of PRMI-1640 with Hepes (GIBCO) supplemented with 5% of heat-inactivated human serum (Swiss Red Cross, Basel, Switzerland), 2mM L-Glutamine (Biochrom), 50U/ml penicillin and 50μg/ml streptomycin (Bioconcept) and 50 IU/ml IL-2 (Roche). T cells were cultured at a density of 0.25-1x105cells/96 well plate in a total volume of 200μL cell culture medium. Illumina TruSeq Stranded mRNA protocol Illumina TruSeq Stranded mRNA protocol
attributes.tag: ENA first public
attributes.value: 2017-10-02
attributes.tag: ENA last update
attributes.value: 2017-06-06
attributes.tag: External Id
attributes.value: SAMEA104113716
attributes.tag: INSDC center alias
attributes.value: Department of Clinical Research, University of Bern, Switzerland.
attributes.tag: INSDC center name
attributes.value: Department of Clinical Research, University of Bern, Switzerland.
attributes.tag: INSDC first public
attributes.value: 2017-10-02T17:03:26Z
attributes.tag: INSDC last update
attributes.value: 2017-06-06T08:32:04Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-5739:09_08_S46
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: T-helper 9 cell
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: growth condition
attributes.value: clonal culture
attributes.tag: individual
attributes.value: 8
attributes.tag: organism part
attributes.value: blood
attributes.tag: phenotype
attributes.value: CXCR3- CCR8+ CCR6- CCR4+
attributes.tag: sample name
attributes.value: E-MTAB-5739:09_08_S46