title: GH308_MIB-2
organism: Oryza sativa
description: In this test, 9 rectangular plastic basins (length * width * height: 40 cm * 20 cm * 10 cm) with soggy soil were set. In each basin, 10 seeds of GH308, HR05 and TN1 (30 seeds in total) were cultivated in the soil and arranged in three lines as Fig 1 displayed. To ensure the 100% of emergence rate, all tested seeds were healthy and soaked in water for 24 hours before sowing.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-07-09T08:05:09Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-07-01T07:30:31Z
attributes.tag: External Id
attributes.value: SAMEA7030883
attributes.tag: INSDC center name
attributes.value: Fujian Agriculture and Forestry University
attributes.tag: INSDC first public
attributes.value: 2020-07-09T08:05:09Z
attributes.tag: INSDC last update
attributes.value: 2020-07-01T07:30:31Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: s3-5
attributes.tag: collection date
attributes.value: 2017-09
attributes.tag: common name
attributes.value: rice
attributes.tag: estimated size
attributes.value: 400M
attributes.tag: geographic location (country and/or sea)
attributes.value: China
attributes.tag: geographic location (latitude)
attributes.value: 26.00697703
attributes.tag: geographic location (longitude)
attributes.value: 119.3604179
attributes.tag: isolation and growth condition
attributes.value: To ensure the 100% of emergence rate, all tested seeds were healthy and soaked in water for 24 hours before sowing. The 9 basins of seeds would kept grow in greenhouse for 10 days, with conditions of 14:10 photoperiod and temperature of 28��1��. Then the 9 basins of seedlings were divided into 3 groups, 2 treatments and 1 control would be set as follow: Group 1, for each basin, 300 individuals of 3-4 instars of planthopper nymphs were released and they could freely move and feed on the 30 seedlings for next 24 hours. Then 5 stems of each rice cultivar were cut, put into three cryo tubes and store in a -80 �� refrigerator rapidly. The tubes were labeled as GH308-IB, HR05-IB and TN1-IB. The tested brown planthoppers were reared indoor for at least 10 generations, feed with seedlings of TN1during their whole life; Group 2, the stems of each seedling would be prick 10 times randomly every hour in next 24 hours, as simulation of insect piercing, then 5 stems of each rice cultivar were cut, put into three cryo tubes and store in a -80 �� refrigerator rapidly (labeled as GH308-MIB, HR05-MIB and TN1-MIB); Group 3, the last three basins was set as control and treated with nothing. After 24 hours, the stems were collected as above did (labeled as GH308-CK, HR05-CK and TN1-CK)
attributes.tag: number of replicons
attributes.value: 1
attributes.tag: plant developmental stage
attributes.value: matrue
attributes.tag: plant growth medium
attributes.value: soil
attributes.tag: plant structure
attributes.value: leaf
attributes.tag: sample name
attributes.value: s3-5
attributes.tag: scientific_name
attributes.value: Oryza sativa