organism: Saccharomyces cerevisiae
description: Genome sequencing of sorted cells of the indicated mutant at the indicated phase
attributes.tag: strain
attributes.value: BY4741
attributes.tag: minutes after releast
attributes.value: not applicable
attributes.tag: collection_date
attributes.value: 2015-03-01
attributes.tag: geo_loc_name
attributes.value: missing
attributes.tag: sample_type
attributes.value: whole organism
attributes.tag: biomaterial_provider
attributes.value: Weizmann Institute of Science, Rehovot, Israel
attributes.tag: genotype
attributes.value: BY4741 mrc1D::NAT
attributes.tag: mating_type
attributes.value: a
attributes.tag: growth_protocol
attributes.value: The cells were grown in YPD overnight at 30°C, and then inoculated to fresh medium to OD600 of 0.49
attributes.tag: treatment
attributes.value: harvested when reached an OD600 of 0.15-0.2. Cells were washed from the media in order to remove secreted BAR1. Cells were then re-suspended in equal-volume fresh warm YPD with ?-factor in a final concentration of 5 µg/ml, and incubated for 3 hours at 30°C. Cells were washed twice with warm YPD to remove ?-factor and re-suspended in equal-volume of fresh YPD.
attributes.tag: development stage
attributes.value: not applicable
attributes.tag: tissue
attributes.value: not applicable
attributes.tag: host
attributes.value: not applicable
attributes.tag: isolation source
attributes.value: not applicable
attributes.tag: BioSampleModel
attributes.value: Microbe, viral or environmental