title: B16-F10 cell line rep2
organism: Mus musculus
description: Protocols: B16F10 melanoma cell line was purchased in 2010 from the American Type Culture Collection (product: ATCC CRL-6475, lot number: 58078645). Early (third and fourth) passages of cells were used for tumor experiments. Reauthentification of cells has not been done since receipt. RNA from B16F10 cells was extracted in triplicate. Using 5 ug of total RNA, barcoded mRNA-seq cDNA libraries were prepared in triplicate using a modified Illumina mRNA-seq protocol.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2014-02-04T17:01:02Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T17:09:02Z
attributes.tag: External Id
attributes.value: SAMEA2341987
attributes.tag: INSDC center name
attributes.value: TRON-MAINZ
attributes.tag: INSDC first public
attributes.value: 2014-02-04T17:01:02Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T17:09:02Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-2271:B16-F10 cell line rep2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: melanoma cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: sample name
attributes.value: E-MTAB-2271:B16-F10 cell line rep2
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: specimen with known storage state
attributes.value: fresh specimen