title: PDAC030T
organism: Homo sapiens
description: Protocols: two tumor sampling methods from patients with PDAC: 1- surgery for patients with tumor size < 2cm, and 2- Endoscopic Ultrasound-Guided Fine-Needle Aspiration (EUS-FNA) for patients with tumor size > 2cm. this information is given by the \Resectable\ variable. For operated patients (with surgery), tumor tissues were xenografted directly. However, biopsy tissues were grown in culture for 1 or 2 days, before transplanted to the nude mouse. The final xenograft samples were collected from mouse when tumors reached a size of 1 cm. The \Differentiation\, \Fibrosis\, \Inflammation\ information are estimated on the collected xenograft tissues. miRNA-Seq libraries are performed from at least 1ug of extracted total RNA with a RIN greater than 7. Before starting, total RNAs are purified with Trizol-GT & miRNeasy kit wich allows the selection of the small RNA fraction less than 100b.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2017-12-12T17:02:53Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2017-03-07T13:50:27Z
attributes.tag: External Id
attributes.value: SAMEA103893919
attributes.tag: INSDC center name
attributes.value: Programme Carte dâ€™Identite des Tumeurs
attributes.tag: INSDC first public
attributes.value: 2017-12-12T17:02:53Z
attributes.tag: INSDC last update
attributes.value: 2017-03-07T13:50:27Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: ProportionMurineRNA
attributes.value: 0.4
attributes.tag: Submitter Id
attributes.value: E-MTAB-5018:PDAC030T
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: pancreatic ductal adenocarcinoma
attributes.tag: individual
attributes.value: PDAC030
attributes.tag: organism status
attributes.value: dead
attributes.tag: resectable
attributes.value: false
attributes.tag: sample name
attributes.value: E-MTAB-5018:PDAC030T
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: male
attributes.tag: survival time
attributes.value: 6
attributes.tag: xenograft
attributes.value: pancreatic cancer cells grown in nude mouse
attributes.tag: xenograft CIMPclass
attributes.value: true
attributes.tag: xenograft MultiOmicsClassification
attributes.value: classical
attributes.tag: xenograft differentiation
attributes.value: non-mucinous
attributes.tag: xenograft fibrosis
attributes.value: true
attributes.tag: xenograft inflammation
attributes.value: true