title: CHT_control_24h_2R
organism: Cucumis melo
description: Protocols: sample growt protocol: melon seedlings were cultivated in plastic pots filled with sterilized soil in the greenhouse at 25±2 °C with 80-90% relative humidity. The FOM1.2 strain ISPaVe1018 was obtained from the fungal collection of the \Centro di ricerca per la patologia vegetale\ (CREA-PAV, Rome). The inoculum preparation and artificial inoculation procedures were reported in Sestili et al. (2011). After root trimming, the plantlets were dipped for 30 min in the conidial suspension. Mock-inoculations were performed in sterile distilled water. Seedlings were transferred into plastic pots filled with sterilized soil and maintained in the greenhouse at 25±2 °C with 80-90% relative humidity. Stems of ten plants per each time point (24 and 48 hpi) were pooled and used for the transcriptome analysis. In addition, 50 plants for each genotype were phenotypically screened at 8, 15, 21 and 35 dpi. Total RNA was isolated from melon infected and mock-treated plants at 24 and 48 hpi using the TRIzol reagent (Invitrogen) and purified by the Qiagen RNeasy® Minikit (Qiagen, Valencia, CA, USA; http://www.qiagen.com), according to manufacturer's instructions. Two biological replicates for each genotype and treatment were used according to recommended RNA-Seq standards.
attributes.tag: ENA first public
attributes.value: 2017-09-30
attributes.tag: ENA last update
attributes.value: 2016-09-29
attributes.tag: External Id
attributes.value: SAMEA4471225
attributes.tag: INSDC center alias
attributes.value: CREA-GPG
attributes.tag: INSDC center name
attributes.value: CREA-GPG
attributes.tag: INSDC first public
attributes.value: 2017-09-30T17:02:11Z
attributes.tag: INSDC last update
attributes.value: 2016-09-29T12:19:53Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-4939:CHT_control_24h_2R
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: muskmelon
attributes.tag: genotype
attributes.value: CHT
attributes.tag: infect
attributes.value: control
attributes.tag: sample name
attributes.value: E-MTAB-4939:CHT_control_24h_2R
attributes.tag: time
attributes.value: 24