title: BSL2012.002_2012-04-17_BLE-LTER_KAA_Sediment_0-3 cm
organism: marine sediment metagenome
attributes.tag: collection_date
attributes.value: 2012-04-17
attributes.tag: depth
attributes.value: 0-3 cm
attributes.tag: env_broad_scale
attributes.value: polar [ENVO:01000339]
attributes.tag: env_local_scale
attributes.value: ocean [ENVO:00000015]
attributes.tag: env_medium
attributes.value: marine sediment [ENVO:03000033]
attributes.tag: geo_loc_name
attributes.value: USA: Kaktovik Lagoon, Alaska
attributes.tag: lat_lon
attributes.value: 70.08905 N 143.62332 W
attributes.tag: samp_collect_device
attributes.value: Sediment was collected using a small Van Veen grab and then a 3 mL subsample was collected for microbial analyses using a syringe core made out of a 10 mL syringe and placed into a 15 mL falcon tube
attributes.tag: samp_mat_process
attributes.value: Sediment was homogenized using sterile spatuala and weighed out into MoBio PowerSoil bead tubes before freezing in the field. In the lab, sample was extracted using MoBio PowerSoil DNA extraction kit
attributes.tag: samp_size
attributes.value: 0.252 g
attributes.tag: site_name
attributes.value: KAA
attributes.tag: min_filter_pore_size
attributes.value: not applicable
attributes.tag: max_filter_pore_size
attributes.value: not applicable
attributes.tag: sequencing_method
attributes.value: Illumina
attributes.tag: sequencing_machine
attributes.value: MiSeq (Illumina)
attributes.tag: BioSampleModel
attributes.value: MIMARKS.survey
attributes.tag: BioSampleModel
attributes.value: MIGS/MIMS/MIMARKS.water