title: genome
organism: Homo sapiens
description: Protocols: Ewing Sarcoma cell lines CADO-ES1, was purchased form DSMZ. Ewing sarcoma cell lines were cultivated in RPMI1640 medium, supplemented with 10% (v/v) FCS, 1% Glutamin and 1% of an antibiotic cocktail containing penicillin and streptomycin. All Ewing sarcoma cell lines were maintained on cell culture plastic coated with collagen (0,5mg/ml). Culture media was supplemented by 1% of an antibiotic cocktail containing penicillin and streptomycin and incubated at 37°C and 5%CO2 in a humidified atmosphere. Routine microbiological analysis was done using a PCR Mycoplasma Detection Kit according to suggested protocols (Applied Biosystems, Darmstadt, Germany). The extraction of nucleic acids from cell cultures was performed by the use of the DNeasy Kit (Qiagen) according to the supplier's recommendations. For DNA library preparation 500 - 5000ng DNA were used. ERCCs as an additional internal quality control were added to the libraries. We followed exactly the Thermo-Fischer/Life-Technology(ABI) protocols for the SOLID-5500XL Technology for DNAseq. Most of the procedures were run in a core lab facility of Life-Technology as a commercial service.
attributes.tag: ENA first public
attributes.value: 2017-10-02
attributes.tag: ENA last update
attributes.value: 2017-09-22
attributes.tag: External Id
attributes.value: SAMEA104313571
attributes.tag: INSDC center alias
attributes.value: University of Muenster, Medical Faculty, Institute of Bioinformatics
attributes.tag: INSDC center name
attributes.value: University of Muenster, Medical Faculty, Institute of Bioinformatics
attributes.tag: INSDC first public
attributes.value: 2017-10-02T17:03:33Z
attributes.tag: INSDC last update
attributes.value: 2017-09-22T15:45:39Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6111:genome
attributes.tag: age
attributes.value: 19
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: CADO ES1
attributes.tag: cell type
attributes.value: Ewings sarcoma
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: Ewing sarcoma
attributes.tag: genotype
attributes.value: EWS-ERG
attributes.tag: metastatic site
attributes.value: pleural fluid
attributes.tag: organism part
attributes.value: bone
attributes.tag: sample name
attributes.value: E-MTAB-6111:genome
attributes.tag: sex
attributes.value: female