title: Pokkali_control_5hrs_rep2
organism: Oryza sativa Indica Group
description: Protocols: Oryza sativa L. Indica Pokkali seedlings were grown in growth chambers to the -3 leaf stage. At 2 weeks old, they were subjected to salt stress treatment by watering with 300 mM NaCl solution (or normal watering for control plants). Treatment was applied at 10:00 PST (2 hours post-dawn). Seedling shoots (stem and leaves) were sampled at 1 hour, 2 hours, 5 hours, 10 hours, and 24 hours post-treatment. Total cellular RNA was extracted using RNA Plant Reagent (Life Technologies) and treated with RNase-free Turbo DNase (Life Technologies) followed by a clean-up step using Plant RNeasy kits (Qiagen). Concentration, integrity and extent of contamination by ribosomal RNA were assessed using ND-1000 spectrophotometer (Thermo Fisher Scientific) and Bioanalyzer 2100 (Agilent Technologies). For details see Fox et al. (2009) Applications for Ultra-high-Throughput Sequencing. In: Belostotsky (ed.), Plant Systems Biology, vol. 553, pp. 79--108. Libraries were prepared using the TruSeq(TM) RNA Sample Preparation Kits (v2).
attributes.tag: ENA first public
attributes.value: 2017-10-02
attributes.tag: ENA last update
attributes.value: 2015-11-30
attributes.tag: External Id
attributes.value: SAMEA3681614
attributes.tag: INSDC center alias
attributes.value: OREGON STATE UNIVERS
attributes.tag: INSDC center name
attributes.value: Oregon State University
attributes.tag: INSDC first public
attributes.value: 2017-10-02T17:05:06Z
attributes.tag: INSDC last update
attributes.value: 2015-11-30T15:38:26Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-1914:Pokkali_control_5hrs_rep2
attributes.tag: age
attributes.value: 2 week
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: long-grained rice
attributes.tag: cultivar
attributes.value: Pokkali
attributes.tag: developmental stage
attributes.value: seedling, two leaves visible, three leaves visible
attributes.tag: organism part
attributes.value: shoot, vascular leaf
attributes.tag: sample name
attributes.value: E-MTAB-1914:Pokkali_control_5hrs_rep2
attributes.tag: specimen with known storage state
attributes.value: fresh specimen