title: sip54_Tot1
organism: Homo sapiens
description: Protocols: Human embryonic kidney HEK293 cells were maintained in DMEM supplemented with 10% (v/v) fetal calf serum. siRNA transfections were performed using Lipofectamin 2000 (Life Technologies, France) according to the manufacturer's protocol. Cells were harvested 48h post-transfection Total and polysomial RNA was extracted using the SV Total RNA Isolation System (Promega) according to the manufacturers instructions Library preparation was performed at the Ecole Normale Superieure Genomic Platform (Paris, France). Ribosomal RNA was depleted from 1 g of total or polysomial RNA using the Ribo-Zero kit Human/Mouse/Rat (Epicentre). Libraries were prepared using the strand specific RNASeq library preparation ScriptSeq V2 kit (Epicentre), and multiplexed by three on flow cell lanes.
attributes.tag: ENA first public
attributes.value: 2017-09-28
attributes.tag: ENA last update
attributes.value: 2015-11-30
attributes.tag: External Id
attributes.value: SAMEA3682502
attributes.tag: INSDC center alias
attributes.value: UPMC, IBPS CNRS-UMR7622, LBD Equipe Compartimentation et trafic intracellulaire des mRNP Case courrier 24
attributes.tag: INSDC center name
attributes.value: UPMC, IBPS CNRS-UMR7622, LBD Equipe Compartimentation et trafic intracellulaire des mRNP Case courrier 24
attributes.tag: INSDC first public
attributes.value: 2017-09-28T17:02:02Z
attributes.tag: INSDC last update
attributes.value: 2015-11-30T17:42:32Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-4091:sip54_Tot1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: HEK293
attributes.tag: common name
attributes.value: human
attributes.tag: fraction
attributes.value: Whole cell lysate
attributes.tag: rna interference
attributes.value: DDX6 siRNA
attributes.tag: sample name
attributes.value: E-MTAB-4091:sip54_Tot1
attributes.tag: treatment
attributes.value: siRNA treatment (50pmoles) for 48h