title: CD14N_1D_d28_2_S1
organism: Homo sapiens
description: Protocols: Cells for sequencing were lysed in 350μl RLT plus buffer Venous blood from healthy human volunteers, collected in EDTA tubes, was diluted 1:2 in PBS, layered on top of Lymphoprep solution (Fresenius Kabi) and fractionated by centrifugation to recover the peripheral blood mononuclear cell(PBMC)fraction. CD14+cells were isolated from the human blood PBMC fraction using the MACS huCD14 microbead kit (Miltenyi biotech), according to the manufacturer's protocol. RNA was prepared using the RNeasy mini 200 plus kit (Qiagen) according to the manufacturer's instructions. One microgram of total RNA was used for each library. RNA samples were processed with Illumina TruSeq Stranded mRNA Library prep kit selecting for poly(A) tailed RNA following the manufacturer's recommendations
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-12-04T04:07:53Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-10-13T14:37:18Z
attributes.tag: External Id
attributes.value: SAMEA7425719
attributes.tag: INSDC center name
attributes.value: AZ
attributes.tag: INSDC first public
attributes.value: 2020-12-04T04:07:53Z
attributes.tag: INSDC last update
attributes.value: 2020-10-13T14:37:18Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-9670:CD14N_1D_d28_2_S1
attributes.tag: batch
attributes.value: 2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: R-iPS 1J
attributes.tag: cell type
attributes.value: induced pluripotent stem cell
attributes.tag: cell_type_abbrev
attributes.value: IPSDMo_CD14neg
attributes.tag: common name
attributes.value: human
attributes.tag: donor
attributes.value: 0
attributes.tag: harvest
attributes.value: d28
attributes.tag: run
attributes.value: 1
attributes.tag: sample name
attributes.value: E-MTAB-9670:CD14N_1D_d28_2_S1
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: stimulus_time
attributes.value: no