title: DGE 1-h cold 1
organism: Anthurium andraeanum
description: Protocols: Micropropagated plants were transferred and grown in the greenhouse for three month in Zhejiang academy of agricultural sciences. Total RNA was isolated using TRIzol reagent (Invitrogen, Carlsbad, CA) according to the manufacturer's instructions. Poly (A)+ mRNA was purified with oligo (dT) beads. RNA libraries were prepared for sequencing using standard Illumina protocols. For RNA Seq, a mixed cDNA sample of control plants and 1, 5, and 24 h cold-treated plants were used for library construction. For DGE, RNAs from control plants and 1, 5, and 24 h cold-treated plants were used for library construction separately.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2014-02-19
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2013-11-05
attributes.tag: External Id
attributes.value: SAMEA2237203
attributes.tag: INSDC center alias
attributes.value: Jiangsu Province and Chinese Academy of Sciences
attributes.tag: INSDC center name
attributes.value: Jiangsu Province and Chinese Academy of Sciences
attributes.tag: INSDC first public
attributes.value: 2014-02-19T16:35:01Z
attributes.tag: INSDC last update
attributes.value: 2013-11-05T11:26:25Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-1955:DGE 1-h cold 1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: developmental stage
attributes.value: seedling
attributes.tag: growth condition
attributes.value: cold air temperature regimen
attributes.tag: organism part
attributes.value: leaves
attributes.tag: sample name
attributes.value: E-MTAB-1955:DGE 1-h cold 1
attributes.tag: specimen with known storage state
attributes.value: frozen specimen
attributes.tag: temperature
attributes.value: 6 degree celsius
attributes.tag: time
attributes.value: 1 hour