title: LPS_1
organism: Homo sapiens
description: Protocols: We used the THP-1 monocytic cell line obtained from ATCC (#TIB-202). Approximately 1 million cells were used for each biological replicate. THP-1 cells were cultured in RPMI 1640 medium (Biochrom, #FG1215) supplemented with 10% fetal bovine serum (FBS, Biochrom, #S0615). All treatment compounds were added to the standard growth medium. THP-1 monocytes were differentiated to macrophages with 10 nM phorbol-12-myristate-13-acetate (PMA, Sigma, #P8139-5MG) for 48 hours. Following differentiation, cells were stimulated with 100 ng/ml LPS (Sigma-Aldrich, #L5293-2ML) for 2 hours, with 250 µM palmitate (Sigma Aldrich, #P9767) for 24 hours in the presence of 10 nM PMA or left unstimulated. RNA extraction was performed using RNeasy Mini Kit from Qiagen (#74106) using the standard procedure with 30 min on-column DNase digestion. RNA concentration and quality was controlled using NanoDrop 2000 (Thermo Fisher Scientific) and Bioanalyser 2100 (Agilent). Sequencing libraries were prepared using TruSeq Stranded mRNA Library Prep Kit (Illumina, #RS-122-2001) according to the standard procedure.
attributes.tag: ENA first public
attributes.value: 2017-09-28
attributes.tag: ENA last update
attributes.value: 2017-09-18
attributes.tag: External Id
attributes.value: SAMEA104305951
attributes.tag: INSDC center alias
attributes.value: Max Delbruck Center for Molecular Medicine in the Helmholtz Association (MDC); Free University of Berlin
attributes.tag: INSDC center name
attributes.value: Max Delbruck Center for Molecular Medicine in the Helmholtz Association (MDC); Free University of Berlin
attributes.tag: INSDC first public
attributes.value: 2017-09-28T17:01:31Z
attributes.tag: INSDC last update
attributes.value: 2017-09-18T15:35:24Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6064:LPS_1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: THP-1
attributes.tag: cell type
attributes.value: macrophage
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: acute monocytic leukemia
attributes.tag: organism part
attributes.value: blood
attributes.tag: progenitor cell type
attributes.value: monocyte
attributes.tag: replicate
attributes.value: 1
attributes.tag: sample name
attributes.value: E-MTAB-6064:LPS_1