title: UG4
organism: Mus musculus
description: Protocols: Sterile Ti discs were arrayed in an untreated 24-well cell culture plate using a block design, with 12 coarse Grain and 12 ultrafine grain disks. Wells were re-fed after 24 hours. After 72 hours Ti discs were transferred to new plates for standard cell harvesting protocols. The cells from four wells were consolidated into one sample resulting in multiple biological replicates for each sample. Substrate for growth: Commercially pure Grade 4 titanium Carpenter Technology Corporation (Reading, Pennsylvania), subjected to Equal Channel Angular Pressing-Conform (ECAP-C), 8 passes of route Bc at 200 C° to 350 C° RNA was extracted with a Direct-zol RNA Mini Prep kit using the standard protocol (Zymo Research Corp., R2050) and included the treatment of the RNA with DNase I to eliminate genomic DNA. RNA concentrations and quality were determined using a Nanodrop 2000 UV-Vis Spectrophotometer (Thermo Scientific). RNA concentrations were confirmed with the Qubit RNA HS assay kit (ThermoFisher Scientific, Q32852). RNA size and integrity was measured using a 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA). Ribosomal RNA subtraction was done using the Ribo-Zero® Gold rRNA Removal Kit (Illumina, San Diego). Next generation sequencing libraries preparation utilized an Illumina TruSeq™ RNA Sample Prep Kit v2 (Illumina, San Diego). Unique indexes were added to each sample during library construction.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-11-26T04:03:05Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-12-17T12:46:27Z
attributes.tag: External Id
attributes.value: SAMEA5182438
attributes.tag: INSDC center name
attributes.value: New Mexico Tech, Biology Department
attributes.tag: INSDC first public
attributes.value: 2019-11-26T04:03:05Z
attributes.tag: INSDC last update
attributes.value: 2018-12-17T12:46:27Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7528:UG4
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: MC3T3-E1 Subclone 4
attributes.tag: cell type
attributes.value: fibroblast
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: embryonic
attributes.tag: disease
attributes.value: normal
attributes.tag: for more information check
attributes.value: https://cbit.maastrichtuniversity.nl/
attributes.tag: growth condition
attributes.value: ultrafine grain titanium
attributes.tag: organism part
attributes.value: calvaria
attributes.tag: sample name
attributes.value: E-MTAB-7528:UG4
attributes.tag: scientific_name
attributes.value: Mus musculus