title: Sample 4
organism: Mus musculus
description: Protocols: wild-type mice and Nkx3.2-Cre;Tcf7l2 f/f mice were generated and their pancreatic tissues dissected at 10 weeks of age For cell isolation, dissected pancreatic tissues were digested with Collagenase P (0.4 mg/ml; Roche) and DNase (0.1 ng/ml; Sigma) diluted in HBSS for 30 minutes at 37 ̊C to obtain single cell. DAPI (1 l/ml; Sigma-Aldrich) was added to label dead cells. Live fluorescently-labeled cells were collected using FACS Aria (BD). Using PureLink RNA micro kit RNA was amplified using SMART-Seq v4 Ultra low Input RNA kit for Sequencing (Clontech), followed by Illumina sequencing library preparation
attributes.tag: ENA first public
attributes.value: 2017-12-14
attributes.tag: ENA last update
attributes.value: 2017-02-02
attributes.tag: External Id
attributes.value: SAMEA72881668
attributes.tag: INSDC center alias
attributes.value: Dept. of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel
attributes.tag: INSDC center name
attributes.value: Dept. of Cell and Developmental Biology, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel
attributes.tag: INSDC first public
attributes.value: 2017-12-14T17:03:03Z
attributes.tag: INSDC last update
attributes.value: 2017-02-02T10:48:38Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-5325:Sample 4
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: pericyte
attributes.tag: common name
attributes.value: house mouse
attributes.tag: genetic modification
attributes.value: targeted mutation
attributes.tag: genotype
attributes.value: Nkx3.2-Cre;EYFP;Tcf7l2 flox/flox
attributes.tag: organism part
attributes.value: pancreas
attributes.tag: sample name
attributes.value: E-MTAB-5325:Sample 4
attributes.tag: strain
attributes.value: C57BL/6