title: untreated_GFPplus_B
organism: Mus musculus
description: Protocols: ES cells were cultured in DMEM with GlutaMax (Life technologies) containing 15% FCS and LIF Lipofectamine RNAi MAX (Life technologies) were used to transfect siRNA. Total RNA was extracted with GenElute total RNA miniprep kit, treated with turbo DNase and purified with RNeasy MinElute Cleanup kit. The quality was assessed on a bioanalyzer. Libraries were prepared with Truseq Stranded Total RNA with Ribo-Zero Gold Prep kit (Illumina).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2015-08-02T17:01:21Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T23:26:31Z
attributes.tag: External Id
attributes.value: SAMEA3250459
attributes.tag: INSDC center name
attributes.value: Max Plank Institute for Molecular Biomedicine
attributes.tag: INSDC first public
attributes.value: 2015-08-02T17:01:21Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T23:26:31Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-2684_2:untreated_GFPplus_B
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: ES-E14
attributes.tag: cell type
attributes.value: embryonic stem cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: genotype
attributes.value: MERVL-LTR::EGFP reporter construct
attributes.tag: phenotype
attributes.value: FACS-sorted EGFP positive
attributes.tag: sample name
attributes.value: E-MTAB-2684_2:untreated_GFPplus_B
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: strain
attributes.value: 129/Ola