organism: Tigriopus californicus
description: F2 hybrid clutches were hatched together in petri dishes, and individuals scored for date of metamorphosis into copepodid stage, and each frozen separately. After ~3000 individuals were scored, the 10% slowest were pooled for library prep and sequencing. Library was prepared using the Illumina Nextera kit, with gel size-selection in the range 350-500bp.
attributes.tag: isolate
attributes.value: hybrid
attributes.tag: isolation-source
attributes.value: intertidal pool
attributes.tag: collection-date
attributes.value: 2018-06
attributes.tag: geo-loc-name
attributes.value: USA
attributes.tag: tissue
attributes.value: whole animal
attributes.tag: age
attributes.value: 12+ days
attributes.tag: depth
attributes.value: intertidal
attributes.tag: dev-stage
attributes.value: adult
attributes.tag: sex
attributes.value: mixed
attributes.tag: temp
attributes.value: 20
attributes.tag: experimental replicate
attributes.value: SHfxSDm F2 slow-growing
attributes.tag: BioSampleModel
attributes.value: Invertebrate