title: sgi6_R2
organism: Arabidopsis thaliana
description: Protocols: 17-day-old (DAS) shoot tissues of plants were harvested. Shoot tissues, consist of stems including the leaves, were harvested and immediately stored in liquid nitrogen. Seedlings were grown on half strength (1/2) MS medium containing 1% sucrose at 21˚C under a 16 h/8 h day/night condition. RNA samples for small RNA sequencing analyses were extracted from 17-day-old shoot tissues of plants by the miRNeasy Mini Kit (Qiagen). Small RNAs (<50 nt) were gel-purified to build the libraries with TruSeq small RNA sample prep kit (illumina).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-05-03T04:03:17Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-04-23T13:24:54Z
attributes.tag: External Id
attributes.value: SAMEA5574670
attributes.tag: INSDC center name
attributes.value: Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, Belgium VIB Center for Plant Systems Biology, VIB, Gent, Belgium
attributes.tag: INSDC first public
attributes.value: 2019-05-03T04:03:17Z
attributes.tag: INSDC last update
attributes.value: 2019-04-23T13:24:54Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7874:sgi6_R2
attributes.tag: age
attributes.value: 17
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: thale cress
attributes.tag: developmental stage
attributes.value: 1 main shoot growth stage
attributes.tag: genotype
attributes.value: EMS mutation of RST1 in ERF6-GR background
attributes.tag: organism part
attributes.value: shoot
attributes.tag: replicate
attributes.value: biological replicate
attributes.tag: sample name
attributes.value: E-MTAB-7874:sgi6_R2
attributes.tag: scientific_name
attributes.value: Arabidopsis thaliana
attributes.tag: ecotype
attributes.value: Col-0