title: BSL2012.116_2012-08-09_BLE-LTER_CR_River_0.01 m
organism: freshwater metagenome
attributes.tag: collection_date
attributes.value: 2012-08-09
attributes.tag: depth
attributes.value: 0.01 m
attributes.tag: env_broad_scale
attributes.value: polar [ENVO:01000339]
attributes.tag: env_local_scale
attributes.value: fresh water [ENVO:00002011]
attributes.tag: env_medium
attributes.value: river water [ENVO:01000599]
attributes.tag: geo_loc_name
attributes.value: USA: Canning River, Alaska
attributes.tag: lat_lon
attributes.value: 70.05937 N 145.56938 W
attributes.tag: samp_collect_device
attributes.value: Collected by dipping a 2L amber bottle into the surface of the river from shore
attributes.tag: samp_mat_process
attributes.value: DNA extracted with phenol-chloroform following Crump et al. (2013) DOI: 10.1128/AEM.69.4.2253-2268.2003
attributes.tag: samp_size
attributes.value: 630 mL
attributes.tag: site_name
attributes.value: CR
attributes.tag: min_filter_pore_size
attributes.value: 0.22 μm (Sterivex-GP)
attributes.tag: max_filter_pore_size
attributes.value: River
attributes.tag: sequencing_method
attributes.value: Illumina
attributes.tag: sequencing_machine
attributes.value: MiSeq (Illumina)
attributes.tag: BioSampleModel
attributes.value: MIMARKS.survey
attributes.tag: BioSampleModel
attributes.value: MIGS/MIMS/MIMARKS.water