title: ROMEO2016.001.DNA_2016-02-03_Atlam_A_winter_river_water
organism: freshwater metagenome
description: USGS 02226160 Altamaha River at Everett City, GA
attributes.tag: isolation_source
attributes.value: river water
attributes.tag: collection_date
attributes.value: 2016-02-03
attributes.tag: geo_loc_name
attributes.value: USA: Altamaha River at Everett City, GA
attributes.tag: lat_lon
attributes.value: 31.427170 N 81.605390 W
attributes.tag: samp_collect_device
attributes.value: Collected into a 10L carboy dipped below the surface from a pier on the right bank of the river channel
attributes.tag: samp_mat_process
attributes.value: Material pre-filtered with a 3.0 micron pore-size, 142mm diameter polycarbonate filter, and collected on a 0.22 micron pore-size Sterivex filter (Millipore, Burlington, MA). Genomic DNA from Thermus thermophilus HB8 was added (44 ng) prior extraction for quantification analyses, as per Satinsky et al. 2013 (https://doi.org/10.1016/B978-0-12-407863-5.00012-5). DNA was extracted using MasterPure™ Complete DNA and RNA Purification Kit (Lucigen, Middleton, WI).
attributes.tag: samp_size
attributes.value: 0.5 L
attributes.tag: isolate
attributes.value: Atlam_A_winter
attributes.tag: replicate
attributes.value: replicate A
attributes.tag: BioSampleModel
attributes.value: Metagenome or environmental