organism: Quercus suber
description: Root segments taken 1cm below root collar were collected after 24h of treatment, frozen in liquid nitrogen and stored. RNA extraction was performed according to Reid et al. 2006 (doi: 10.1186/1471-2229-6-27). cDNA was synthesized using SMARTSeq2 and sequenced on Illumina NextSeq 500 instrument (75 bp, Single Reads)
attributes.tag: cultivar
attributes.value: not applicable
attributes.tag: age
attributes.value: 8 days (after radicle emergence)
attributes.tag: dev_stage
attributes.value: Secondary development
attributes.tag: geo_loc_name
attributes.value: Portugal: Alcochete
attributes.tag: tissue
attributes.value: Root
attributes.tag: biomaterial_provider
attributes.value: Pedro Barros, ITQB NOVA
attributes.tag: collected_by
attributes.value: Ana Rita Leal
attributes.tag: collection_date
attributes.value: 2018-02
attributes.tag: growth_protocol
attributes.value: Seeds were sown on a mixture of sand and vermiculite (1:1) at 100% field capacity in 700ml pots and maintained under 12h photoperiod at 25ºC/20ºC (day/night). Treatments were applied after 7 days and taproot samples were taken after 24h.
attributes.tag: sample_type
attributes.value: 1cm root segments, collected 1cm below root collar (pooled from 4-5 acorns)
attributes.tag: temp
attributes.value: 25ºC
attributes.tag: treatment
attributes.value: After 7 days of germination, acorns were re-watered to 80% field capacity and maintained at 25ºC/20ºC day/night (Mock/Control).
attributes.tag: replicate
attributes.value: biological replicate 2
attributes.tag: BioSampleModel
attributes.value: Plant