title: NTC-Rep5
organism: Mus musculus
description: Well differentiated myotubes were treated with NTC_2
attributes.tag: strain
attributes.value: C57BL6
attributes.tag: age
attributes.value: Day 7 after myoblast was differentiate as myobutes
attributes.tag: dev_stage
attributes.value: Well differentiated myotubes
attributes.tag: sex
attributes.value: not determined
attributes.tag: tissue
attributes.value: Skeletal muscle
attributes.tag: cell_line
attributes.value: C2C12
attributes.tag: cell_subtype
attributes.value: Well differentiated myotubes
attributes.tag: cell_type
attributes.value: Well differentiated myotubes
attributes.tag: collection_date
attributes.value: 2020-06
attributes.tag: disease
attributes.value: Muscle wasting
attributes.tag: genotype
attributes.value: WT
attributes.tag: growth_protocol
attributes.value: C2C12 myoblasts with <15 passages were induced by gentle replacement of the culture medium with fusion medium (DMEM with 2% horse serum and 1% penicillin/streptomycin). When the C2C12 myoblasts in the growth medium reached about 80%-90% confluence, a substantial volume of the medium was added, and incubation was continued for 4 days. The differentiation medium was changed every 3-4 days after myotubes were formed and used for the intervention experiment. Cell culture-based experiments were performed in independent triplicates.
attributes.tag: health_state
attributes.value: Good
attributes.tag: phenotype
attributes.value: Well differentiated myotubes_2
attributes.tag: sample_type
attributes.value: Total RNA
attributes.tag: treatment
attributes.value: negative treatment control_2
attributes.tag: BioSampleModel
attributes.value: Model organism or animal