title: HL60_d2_input_2
organism: Homo sapiens
description: Protocols: 10^7 HL-60 cells treated with 10 uM ATRA for 2 and 5 days were harvested for ChIP-seq. 10% whole cell lysates were saved as input after genomic DNA was broken into 200-500 bp by sonication. 1 μg IP grade antibodies of CTCF, H3K4me3 or H3K27me3 (CST, Boston, USA) were incubated with the rest of the lysate overnight, followed by 2 h protein-A beads incubation at 4 °C for target protein pull down. The CTCF enriched or H3K4/27me3 modified DNA or input DNA were repaired to 3'-dA overhang and added the ligated adapter. The DNA library was eliminated the unligated adapters and selected the appropriate size for sequence using an Illumina X Ten platform.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-05-07T17:05:44Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-12-04T15:13:12Z
attributes.tag: External Id
attributes.value: SAMEA6360278
attributes.tag: INSDC center name
attributes.value: Department of Molecular Pathology, The Affiliated Cancer Hospital of Zhengzhou University, Henan Cancer Hospital, Zhengzhou, Henan 450008, PR. China
attributes.tag: INSDC first public
attributes.value: 2020-05-07T17:05:44Z
attributes.tag: INSDC last update
attributes.value: 2019-12-04T15:13:12Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8558:HL60_d2_input_2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: HL-60
attributes.tag: cell type
attributes.value: leukocyte
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: acute myeloid leukemia
attributes.tag: genotype
attributes.value: somatic genotype
attributes.tag: organism part
attributes.value: blood
attributes.tag: sample name
attributes.value: E-MTAB-8558:HL60_d2_input_2
attributes.tag: scientific_name
attributes.value: Homo sapiens