title: Invertebrate sample from Thaumetopoea pityocampa
organism: Thaumetopoea pityocampa
description: This BioSample describes Thaumetopoea pityocampa Reads from a portuguese population called WP (winter population). These reads are used to construct a reference transcriptome (see bioproject). All the material was sampled between 2010 and 2012 from the Mata Nacional de Leiria, Portugal Samples from the main developmental stages ) were obtained from laboratory rearing as detailed in Branco, Paiva, Santos, Burban, and Kerdelhue (2017). RNA was extracted using a Trizol extraction procedure. After verification of RNA quality and integrity the final libraries were sequenced using an illumina Hiseq2000 using the paired-end protocole (2 by 100nt).
attributes.tag: isolate
attributes.value: Winter Population (SP)
attributes.tag: isolation_source
attributes.value: pine forest
attributes.tag: collection_date
attributes.value: 2010/2012
attributes.tag: geo_loc_name
attributes.value: Portugal: Mata Nacional de Leiria
attributes.tag: tissue
attributes.value: eggs, L3 and L5 larvae, pupae (early and late), adults
attributes.tag: lat_lon
attributes.value: 39.47 N 8.58 W
attributes.tag: sex
attributes.value: pooled male and female
attributes.tag: BioSampleModel
attributes.value: Invertebrate