title: O2 Fungi
organism: uncultured fungus
description: uncultured fungus from farmyard manure fertilized soil 2
attributes.tag: environment (material)
attributes.value: agricultural soil
attributes.tag: collection date
attributes.value: 2011-22-09
attributes.tag: target gene
attributes.value: internal trancribed spacer
attributes.tag: geographic location (longitude)
attributes.value: 11.8798
attributes.tag: history/tillage
attributes.value: disc plough
attributes.tag: geographic location (elevation)
attributes.value: 118
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2014-03-03T14:25:28Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2016-10-21T09:33:04Z
attributes.tag: storage conditions (fresh/frozen/other)
attributes.value: -20 °C freezer, 6 months
attributes.tag: pH
attributes.value: 6.6
attributes.tag: soil environmental package
attributes.value: soil
attributes.tag: environment (biome)
attributes.value: cropland
attributes.tag: environment (feature)
attributes.value: field
attributes.tag: link to classification information
attributes.value: http://doi.org/10.1002/jpln.200521814
attributes.tag: link to climate information
attributes.value: www.ufz.de/index.php?de=13170
attributes.tag: history/previous land use
attributes.value: row crops rotation since 1902
attributes.tag: amount or size of sample collected
attributes.value: 500
attributes.tag: adapters
attributes.value: CCATCTCATCCCTGCGTGTCTCCGAC, CCTATCCCCTGTGTGCCTTGGCAGT
attributes.tag: mean annual and seasonal precipitation
attributes.value: 540.3
attributes.tag: organism
attributes.value: uncultured soil fungus
attributes.tag: sample collection device or method
attributes.value: push core
attributes.tag: current land use
attributes.value: row crops
attributes.tag: links to additional analysis
attributes.value: www.ufz.de/index.php?de=16763
attributes.tag: geographic location (latitude)
attributes.value: 51.3895
attributes.tag: scientific_name
attributes.value: uncultured soil fungus
attributes.tag: geographic location (country and/or sea,region)
attributes.value: Germany
attributes.tag: target subfragment
attributes.value: ITS1, 5.8S, ITS2 partial
attributes.tag: investigation type
attributes.value: mimarks-survey
attributes.tag: pcr primers
attributes.value: TCCTCCGCTTATTGATATGC, CTTGGTCATTTAGAGGAAGTAA
attributes.tag: mean annual and seasonal temperature
attributes.value: 9.8
attributes.tag: horizon
attributes.value: A horizon
attributes.tag: depth
attributes.value: 0.1
attributes.tag: project name
attributes.value: Multiple barcoding of soil microbial eukaryotes
attributes.tag: sequencing method
attributes.value: pyrosequencing
attributes.tag: history/agrochemical additions
attributes.value: 30 t . ha-1 . 2y-1 farmyard manure 
attributes.tag: history/crop rotation
attributes.value: 8 years rotations ; potatoes, winter wheat, sugar beet, barley, potatoes, winter wheat, alfalfa, alfalfa ; 2004-2011
attributes.tag: composite design/sieving (if any)
attributes.value: composite sample of ten cores pooled before sieving, 2 mm mesh size sieving
attributes.tag: pH method
attributes.value: in H20
attributes.tag: soil_taxonomic/FAO classification
attributes.value: Haplic Chernozem
attributes.tag: multiplex identifiers
attributes.value: ATCAGACACG
attributes.tag: sample weight for DNA extraction
attributes.value: 0.5
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2014-03-03
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2016-10-21