title: EM5
organism: Caenorhabditis elegans
description: Protocols: Young adult worms were collected from NGM-Petri dishes. The external gamma radiation exposure was conducted at the FIGARO 60Co irradiation facility (activity ~420 GBq) at the Norwegian University of Life Sciences (NMBU) (Lind et al., 2019). Nematodes were exposed, in triplicate, in liquid media (15 ml tissue-culture flasks or front row 24-well cell culture plates) or on NGM-Petri dishes. During exposure, controls were placed, in triplicate, behind lead shielding, while exposure containers were placed at distances corresponding to a calculated average absorbed dose-rates to water of 0.43 - 1.1 - 10.8 - 40.8 and 99.9 mGy/h. RNA was extracted after 72 hours of exposure from L1 stage on young adult nematodes (n=1000 per replicate, three biological replicates per treatment), nematodes were washed and snap-frozen in LIN (liquid nitrogen) and stored at -80 °C until used. Total RNA was extracted using Direct-zol Reagent (Nordic Biosite) and purified with RNeasy Mini Kit (Zymo Research) according to manufacture instruction. Strand-specific TruSeq™ RNA-seq paired-end libraries were prepared according to manufacture instruction.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-10-17T04:03:25Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-10-07T12:11:13Z
attributes.tag: External Id
attributes.value: SAMEA6056673
attributes.tag: INSDC center name
attributes.value: Centre for Integrative Genetics (CIGENE), Faculty of Biosciences (BIOVIT), Norwegian University of Life Sciences (NMBU), 1432, As, Norway
attributes.tag: INSDC first public
attributes.value: 2019-10-17T04:03:25Z
attributes.tag: INSDC last update
attributes.value: 2019-10-07T12:11:13Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8284:EM5
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: whole organism
attributes.tag: sample name
attributes.value: E-MTAB-8284:EM5
attributes.tag: scientific_name
attributes.value: Caenorhabditis elegans
attributes.tag: sex
attributes.value: hermaphrodite
attributes.tag: strain
attributes.value: N2