title: 8-0_ct_ev_but_S27
organism: Mus musculus
description: Protocols: Splenic CD21hiCD24hi B cells were isolated from Mb1cre and Ahrfl/-Mb1cre mice in the remission phase of arthritis. Sorted cells were either left untouched (ex-vivo) or stimulated with LPS+anti-IgM for 6h in IMDM media. Dead cells were excluded using DAPI. Butyrate: 150mM; LPS: 10 mg/ml; IgM; 10 ug/ml Total RNA was isolated from these populations using the Picopure RNA isolation kit (ThermoFisher Scientific), according to manufacturer's instructions. 130-500ng of total RNA was fragmented followed by reverse transcription and second strand cDNA synthesis. 130-500ng of total RNA was fragmented followed by reverse transcription and second strand cDNA synthesis. The double strand cDNA was subjected to end repair, A base addition, adapter ligation and PCR amplification to create libraries. Libraries were evaluated by Qubit and TapeStation. Sequencing libraries were constructed with barcodes to allow multiplexing of samples in 3 lanes.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-10-15T04:05:16Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-10-14T16:30:15Z
attributes.tag: External Id
attributes.value: SAMEA6082039
attributes.tag: INSDC center name
attributes.value: Bering Limited
attributes.tag: INSDC first public
attributes.value: 2019-10-15T04:05:16Z
attributes.tag: INSDC last update
attributes.value: 2019-10-14T16:30:15Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-73451571066817:8-0_ct_ev_but_S27
attributes.tag: age
attributes.value: 6 to 12
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: B cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: genotype
attributes.value: Mb1+/cre
attributes.tag: organism part
attributes.value: spleen
attributes.tag: phenotype
attributes.value: wild type
attributes.tag: sample name
attributes.value: E-MTAB-73451571066817:8-0_ct_ev_but_S27
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: not available
attributes.tag: strain
attributes.value: C57BL/6