title: H3K27ac_168h_rep2
organism: Homo sapiens
description: Protocols: BLaER1 cells at different time points after trasndifferentiation induction were fixed for 10 minutes at room temperature with 1% formaldehyde. Chromatin was sheared by using a Covaris ultrasonicator until fragments ranged from 200 to 1,000 bp. Chromatin immunoprecipitations were performed with 2 ug of chromatin and following standard procedures. BLaER1 cells were grown in RPMI 1640 medium (Gibco) supplemented with 10% inactivated Foetal Bovine Serum (Gibco), 5% Penicillin-Streptomycin (Gibco) and 5% L-Glutamine (Gibco). Cells were maintained at 37ºC in a humid atmosphere containing 5% CO2. Transdifferentiation was induced by addition of 100 nM of beta-estradiol (Calbiochem) and 10 nM of IL-3 and CSF-1 (Peprotech). Immunoprecipitated chromatin was purified with Qiagen PCR purification columns, following the manufacturer's instructions. Sequencing libraries were performed with I ug of ChIP'ped DNA with NebNext ChIP-Seq library kit, following the manufacturer's instructions.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-05-08T17:05:01Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-04-29T20:56:14Z
attributes.tag: External Id
attributes.value: SAMEA6808363
attributes.tag: INSDC center name
attributes.value: CRG, BIST IMIM UPF
attributes.tag: INSDC first public
attributes.value: 2020-05-08T17:05:01Z
attributes.tag: INSDC last update
attributes.value: 2020-04-29T20:56:14Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-9010:H3K27ac_168h_rep2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: BLaER1
attributes.tag: cell type
attributes.value: B cell
attributes.tag: common name
attributes.value: human
attributes.tag: sample name
attributes.value: E-MTAB-9010:H3K27ac_168h_rep2
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: stimulus
attributes.value: beta-estradiol, CSF-1 and IL-3