organism: seawater metagenome
attributes.tag: investigation_type
attributes.value: metagenome
attributes.tag: project_name
attributes.value: Crocker Reef Water Metagenomes
attributes.tag: collection_date
attributes.value: 2015-01-30
attributes.tag: env_package
attributes.value: mims extension
attributes.tag: env_biome
attributes.value: ocean
attributes.tag: env_feature
attributes.value: approximately 20 cm above benthic substrate in seagrass bed
attributes.tag: env_material
attributes.value: water
attributes.tag: geo_loc_name
attributes.value: USA: Florida Keys, Crocker Reef
attributes.tag: lat_lon
attributes.value: 24.91082 N 80.52775 W
attributes.tag: depth
attributes.value: 4.3 m
attributes.tag: samp_collect_device
attributes.value: Peristaltic pump
attributes.tag: samp_mat_process
attributes.value: Water was pumped through a mesh prescreen, a 0.45 um prefilter and a 0.22 um Sterivex filter. Two replicates of approximately 5 L each were sampled. The Sterivex filters were then frozen in liquid nitrogen until the ship returned to shore, where the filters were transferred to freezer at -80 degrees Celsius until DNA extraction in July and August 2015.
attributes.tag: samp_vol_we_dna_ext
attributes.value: 10.0 L
attributes.tag: samp_store_temp
attributes.value: -80 degrees Celsius
attributes.tag: nucl_acid_ext
attributes.value: Manufacturer's protocol for MO BIO PowerWater Sterivex DNA Isolation Kit; modified protocol to eliminate Steps 8 and 9 (heating and cooling steps). Extracted DNA from replicate filters was pooled in order to obtain one extraction for each sample.
attributes.tag: lib_const_meth
attributes.value: TruSeq Nano DNA Library Prep Kit
attributes.tag: seq_meth
attributes.value: Illumina MiSeq v3 chemistry generating paired-end 300-bp reads
attributes.tag: BioSampleModel
attributes.value: MIMS.me
attributes.tag: BioSampleModel
attributes.value: MIGS/MIMS/MIMARKS.water