title: T14_S20
organism: Salmo salar
description: Protocols: Experiment was done in fish lab of Norwegian University of Life Sciences. Fish was slaughtered when they were at ~20g in freshwater. Liver was flash frozen and stored in -80 before RNAseq. RNA from liver tissue was extracted by using RNeasy Plus Universal Mini Kit (Cat No./ID: 73404), following the protocol. RNA concentration was measured by using NanoDrop 8000 (Thermo Scientific, Wilmington, USA) and Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Nucleic acid library was prepared by using TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA, USA), follow the protocol.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-10-18T04:04:46Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-09-05T16:34:56Z
attributes.tag: External Id
attributes.value: SAMEA5937071
attributes.tag: INSDC center name
attributes.value: NORWEGIAN UNIVERSITY OF LIFE SCIENCES
attributes.tag: INSDC first public
attributes.value: 2019-10-18T04:04:46Z
attributes.tag: INSDC last update
attributes.value: 2019-09-05T16:34:56Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8305:T14_S20
attributes.tag: age
attributes.value: 180
attributes.tag: body weight
attributes.value: +/- 20
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: Atlantic salmon
attributes.tag: developmental stage
attributes.value: freshwater
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: individual
attributes.value: T18_S10
attributes.tag: organism part
attributes.value: liver
attributes.tag: sample name
attributes.value: E-MTAB-8305:T14_S20
attributes.tag: scientific_name
attributes.value: Salmo salar
attributes.tag: sex
attributes.value: not available