title: kss_H3K9me2_Rep1_input
organism: Arabidopsis thaliana
description: Protocols: Unopened flower buds at floral stage 9 were collected in tubes, snap frozen in liquid nitrogen and stored at -80°C until further use. Plants were grown under long day conditions (16 hours light/8 hours dark) at 20°C. Two grams of unopened floral buds were ground in liquid nitrogen and used to perform ChIP-seq for histone modifications in wild type, cmt3 or kyp/suvh4 suvh5 suvh6, as described in Choi et al. (2018) Genome Res. 28(4): 532-546. 5 μg of antibodies were used per gram of tissue. The following antibodies were used: α-H3K4me1 (Abcam, ab8895), α-H3K4me2 (Abcam, ab7766), α-H3K9me2 (Abcam, ab1220), α-H3K27me1 (Merck, 07-448) and α-H3K27me3 (Merck, 07-449). 10 ng of ChIP-purified DNA was used to generate libraries using the TruSeq Library Preparation Kit v2 (Illumina), as described in Choi et al. (2018) Genome Res. 28(4): 532-546.
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism
attributes.value: Arabidopsis thaliana
attributes.tag: ecotype
attributes.value: Col-0
attributes.tag: developmental stage
attributes.value: floral organ formation stage
attributes.tag: age
attributes.value: 6
attributes.tag: genotype
attributes.value: kyp/suvh4 suvh5 suvh6
attributes.tag: organism part
attributes.value: flower bud
attributes.tag: growth condition
attributes.value: long day length regimen
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-10-20
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-10-31