title: TARA_20130912T0359Z_194_EVENT_PUMP_DCM_(35m)_SEQ-(100L)_W0.22-3_B110000967
organism: marine metagenome
description: This sample (TARA_B110000967) was collected at station TARA_194 during campaign TARA_20130907Z of the Tara Oceans expedition (2009-2013), using a high volume peristaltic pump [HVP-PUMP]. The sampling event (TARA_20130912T0359Z_194_EVENT_PUMP) occurred at position latitudeN=73.3275 and longitudeE=-168.8142, on date/time=2013-09-12T03:59:00, at a depth of 33-37 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [DCM] deep chlorophyll maximum layer (ENVO:01000326). The sample material was size-fractionated (0.22-3 micrometres) and prepared on board using protocol [SEQ-(100L)_W0.22-3] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of prokaryotes. This sample has replicate sample(s): TARA_B110000968.
attributes.tag: Chlorophyll Sensor
attributes.value: 0.7059
attributes.tag: Citation
attributes.value: Tara Oceans Consortium, Coordinators & Tara Oceans Expedition, Participants. Registry of all samples from the Tara Oceans Expedition (2009-2013). PANGAEA. doi:10.1594/PANGAEA.842197 (2015) (https://dx.doi.org/10.1594/PANGAEA.842197)
attributes.tag: ENA first public
attributes.value: 2019-10-15
attributes.tag: ENA last update
attributes.value: 2016-08-25
attributes.tag: Event Date/Time End
attributes.value: 2013-09-12T04:52:00
attributes.tag: External Id
attributes.value: SAMEA4397903
attributes.tag: INSDC center alias
attributes.value: GSC
attributes.tag: INSDC center name
attributes.value: Genoscope
attributes.tag: INSDC first public
attributes.value: 2019-10-15T04:04:43Z
attributes.tag: INSDC last update
attributes.value: 2016-08-25T12:13:01Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Latitude End
attributes.value: 73.3091
attributes.tag: Longitude End
attributes.value: -168.9031
attributes.tag: Salinity Sensor
attributes.value: 31.56495
attributes.tag: Submitter Id
attributes.value: TARA_B110000967
attributes.tag: depth
attributes.value: 35
attributes.tag: environment (biome)
attributes.value: Polar Biome
attributes.tag: environment (feature)
attributes.value: [DCM] deep chlorophyll maximum layer (ENVO:01000326)
attributes.tag: environment (feature) further details
attributes.value: discrete depth within the epipelagic zone (ENVO:00000209) within the photic zone (ENVO:00000209), <10 m from a strong peak in chlorophyll fluorescence, <50 m below a marine epipelagic wind mixed layer (ENVO:01000061)
attributes.tag: environment (material)
attributes.value: water (ENVO:00002006), including particulate matter (ENVO:01000060) such as planktonic material (ENVO:01000063)
attributes.tag: environmental package
attributes.value: water
attributes.tag: event date/time start
attributes.value: 2013-09-12T03:59:00
attributes.tag: event label
attributes.value: TARA_20130912T0359Z_194_EVENT_PUMP
attributes.tag: further details
attributes.value: http://store.pangaea.de/Projects/TARA-OCEANS/Logsheets_Wetlab/TARA_20130912Z_194_WETLAB_BACT.pdf
attributes.tag: last update date
attributes.value: 2016-04-23Z
attributes.tag: latitude start
attributes.value: 73.3275
attributes.tag: longitude start
attributes.value: -168.8142
attributes.tag: marine region
attributes.value: [AO] Arctic Ocean (MRGID:1906)
attributes.tag: nitrate sensor
attributes.value: -2.647802
attributes.tag: oxygen sensor
attributes.value: 397.421583
attributes.tag: project name
attributes.value: Tara Oceans Expedition (2009-2013)
attributes.tag: protocol description
attributes.value: In the size fraction W0.22-3 micrometres, the sample material was pre-filtered on 3-micrometres, using a 142-mm-diameter Millipore polycarbonate membrane (ref:TSTP14250), and concentrated on 0.22-micrometres, using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane filter (ref:GPWP14250). A volume of 100 Litres was filtered and the 0.22-micrometre-pore-size filter was packaged in a sterile Falcon tube (50 mL), treated with the addition of 10 mL of RNA-Later, labelled with a barcode identification sticker and stored on board in a freezer at -20 degree Celsius. Note 1: Latex or nitril gloves were used for this protocol. Note 2: All containers, filter holders and tubing were washed with 0.1% bleach, rinsed twice with fresh water and rinsed again twice with the pre-filtered sample material. All tweezers are kept clean with ethanol. Note 3: A peristaltic pump (Masterflex, EW-77410-10) was used with a flow rate rendering a pressure of ca. 10-15 psi. Note 4: When the filtration rate decreased considerably, filters were replaced. All filters from one filtration were stored in the same sample container. A total of 2 replicates were prepared and sent for later analysis to the Institute of Marine Science (CSIC), Barcelona, Spain.
attributes.tag: protocol label
attributes.value: SEQ-(100L)_W0.22-3
attributes.tag: sample collection device
attributes.value: high volume peristaltic pump [HVP-PUMP]
attributes.tag: sample material label
attributes.value: TARA_194_DCM_0.22-3
attributes.tag: sample name
attributes.value: TARA_B110000967
attributes.tag: sample status
attributes.value: This version can be used to provide data discovery services
attributes.tag: sampling campaign
attributes.value: TARA_20130907Z
attributes.tag: sampling platform
attributes.value: SV Tara
attributes.tag: sampling station
attributes.value: TARA_194
attributes.tag: size fraction lower threshold
attributes.value: 0.22
attributes.tag: size fraction upper threshold
attributes.value: 3
attributes.tag: temperature
attributes.value: -0.987833