title: Acanthamoeba castellanii strain Neff (ATCC-30010) - mutant (pGAPDH-EGFP)
organism: Acanthamoeba castellanii
description: This sample was used for a sequence-based exploration of transgene integration in Acanthamoeba castellanii. Acanthamoeba castellanii strain Neff (ATCC-30010) cells were transformed with plasmid pGAPDH-EGFP and maintained in PYG medium under selection with G418. No clones were isolated following transformation; the sequence data come from a mixed population of multiple successfully transformed cells. Genomic DNA from the cultures grown from these mixed transformants were sequenced with the Oxford Nanopore MinION.
attributes.tag: strain
attributes.value: Neff (ATCC-30010)
attributes.tag: collection_date
attributes.value: 1957
attributes.tag: env_biome
attributes.value: N/A
attributes.tag: env_feature
attributes.value: N/A
attributes.tag: env_material
attributes.value: soil
attributes.tag: estimated_size
attributes.value: About 42 000 000 bp
attributes.tag: geo_loc_name
attributes.value: USA: Pacific Grove, California
attributes.tag: isol_growth_condt
attributes.value: Axenic growth in PYG-like medium
attributes.tag: lat_lon
attributes.value: 36.62 N 121.92 W
attributes.tag: num_replicons
attributes.value: N/A
attributes.tag: ploidy
attributes.value: unknown
attributes.tag: propagation
attributes.value: eukaryote: unknown sexuality
attributes.tag: isolation_source
attributes.value: soil
attributes.tag: BioSampleModel
attributes.value: MIGS.eu