title: Metagenome or environmental sample from Methanotrophic bacteria consortium
organism: salt mine metagenome
attributes.tag: isolation_source
attributes.value: rocks surrounding the salt deposit in the Wieliczka Salt Mine
attributes.tag: collection_date
attributes.value: 2015-10-10
attributes.tag: geo_loc_name
attributes.value: Poland: Wieliczka
attributes.tag: lat_lon
attributes.value: 49.59 N 20.05 E
attributes.tag: rel_to_oxygen
attributes.value: aerobe
attributes.tag: samp_collect_device
attributes.value: The sampling in the mine was carried out after consultation and with assistance of the mine geologists. The pieces of rock samples were taken from the salt mine, immediately put into sterile polyethylene bags, and transported to the laboratory.
attributes.tag: samp_mat_process
attributes.value: Rock pieces were irradiated under a UV lamp on each side for 20 min. Next, each sample was immersed in ethanol and immediately ignited with a gas burner. Afterwards, the surface rocks layers were removed with a sterile knife, and holes were slowly drilled with a sterile hand-driven drilling machine (Pokorny, 2005)
attributes.tag: samp_size
attributes.value: Rock pieces (approximately 20, 10, 10 cm or larger)
attributes.tag: BioSampleModel
attributes.value: Metagenome or environmental