title: rMMA167
organism: Homo sapiens
description: Protocols: HUVECs cultured in 6-well plates were washed in PBS once and then harvested in 1ml Trizol. HUVECs were isolated from human umbilical cords and cultured in Gelatin coated cell culture dishes, using M199 Medium supplemented with 20% FBS, PenStrep, Glutamine, heparin and endothelial cell growth factors (ECGS, PromoCell). 300000 HUVECs (passage 6) were seeded in 2ml of medium per well of a 6-well plate, treated with lentiviral shCtrl and shB55alpha (Sigma; TRCN0000002489) and cultured in the presence of qVD for 20hrs in Full Medium. RNA was isolated using Trizol and the concentration as well as purity was determined spectrophotometrically using the Nanodrop ND-1000 (Nanodrop Technologies). Per sample, an amount of 3000ng of total RNA was used as input. Subsequently, preparation was performed with KAPA stranded mRNA seq Kit according to the protocol and samples were pooled equimolar. Preparation was performed with KAPA stranded mRNA seq Kit according to the protocol and samples were pooled equimolar.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-05-08T04:03:57Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-04-28T16:07:35Z
attributes.tag: External Id
attributes.value: SAMEA6806901
attributes.tag: INSDC center name
attributes.value: VIB-KU Leuven Center for Cancer Biology Department of Oncology, KU Leuven
attributes.tag: INSDC first public
attributes.value: 2020-05-08T04:03:57Z
attributes.tag: INSDC last update
attributes.value: 2020-04-28T16:07:35Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-9002:rMMA167
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: HUVEC cell line
attributes.tag: cell type
attributes.value: endothelial cell of umbilical vein
attributes.tag: common name
attributes.value: human
attributes.tag: organism part
attributes.value: umbilical vein
attributes.tag: sample name
attributes.value: E-MTAB-9002:rMMA167
attributes.tag: scientific_name
attributes.value: Homo sapiens