title: Environmental/Metagenome sample for Otumba arable soil
organism: soil metagenome
description: Soil microbial biomass has been determined since the mid 1970s by the chloroform fumigation incubation technique as proposed by Jenkinson and Powlson (1976). The microbial biomass C can be determined by subtracting the CO2 emitted from an unfumigated soil (mineralization of soil organic matter) from that emitted from a chloroform fumigated inoculated soil (mineralization of soil organic matter and killed soil microorganisms) and dividing the difference by a mineralization factor (kC = 0.45). The question remained which microorganisms recolonized a fumigated soil. An arable soil was fumigated for one day with ethanol-free chloroform or left unfumigated and incubated aerobically after removal of the chloroform for 10 days. The bacterial population structures were determined in the fumigated and unfumigated soil after 0, 1, 5 and 10 days
attributes.tag: env_package
attributes.value: MIGS/MIMS/MIMARKS.soil
attributes.tag: investigation_type
attributes.value: metagenome
attributes.tag: biome
attributes.value: Arid soil
attributes.tag: collection_date
attributes.value: 13-Jan-2016
attributes.tag: feature
attributes.value: Chloroform Fumigated arable soil
attributes.tag: geo_loc_name
attributes.value: Mexico: Estado de Mexico, Otumba
attributes.tag: lat_lon
attributes.value: 19.41 N 98.43 W
attributes.tag: material
attributes.value: soil
attributes.tag: depth
attributes.value: 20 cm
attributes.tag: elev
attributes.value: 2349 masl
attributes.tag: cur_land_use
attributes.value: small grains
attributes.tag: annual_season_precpt
attributes.value: 577 mm
attributes.tag: annual_season_temp
attributes.value: 14.8
attributes.tag: ph_meth
attributes.value: CaCl2 0.1 M
attributes.tag: ph
attributes.value: 7.6
attributes.tag: pool_dna_extracts
attributes.value: 4
attributes.tag: samp_weight_dna_ext
attributes.value: 0.5 g
attributes.tag: texture_meth
attributes.value: Bouyoucos hydrometer method
attributes.tag: texture
attributes.value: 84, 5, 11, Sandy loam
attributes.tag: tot_n_meth
attributes.value: Kjeldahl
attributes.tag: tot_n
attributes.value: 0.8
attributes.tag: tot_org_c_meth
attributes.value: Combustion and IR analysis
attributes.tag: tot_org_carb
attributes.value: 4.5
attributes.tag: Day
attributes.value: 0
attributes.tag: Replicate
attributes.value: 1
attributes.tag: Treatment
attributes.value: Fumigated
attributes.tag: BioSampleModel
attributes.value: MIMS.me
attributes.tag: BioSampleModel
attributes.value: MIGS/MIMS/MIMARKS.soil