title: moving-bed reactor attached biomass 1A
organism: bioreactor sludge metagenome
description: Bacterial community from bioreactor sludge metagenome
attributes.tag: collection_date
attributes.value: 2015-05-30
attributes.tag: description
attributes.value: Bacterial community from bioreactor sludge metagenome
attributes.tag: env_biome
attributes.value: aerobic bioreactor (ENVO:00002126)
attributes.tag: env_feature
attributes.value: aerobic bioreactor (ENVO:00002126)
attributes.tag: env_material
attributes.value: biofilm (ENVO:00002034)
attributes.tag: geo_loc_name
attributes.value: Brazil:Rio de Janeiro
attributes.tag: lat_lon
attributes.value: 22.86 S 43.23 W
attributes.tag: organism
attributes.value: bioreactor sludge metagenome
attributes.tag: sample_title
attributes.value: moving-bed reactor attached biomass 1A
attributes.tag: seq_methods
attributes.value: The 16S rRNA gene V4 variable region PCR primers 515/806 (Caporaso et al 2011) were used in a single-step 30 cycle PCR using the HotStarTaq Plus Master Mix Kit (Qiagen, USA) under the following conditions: 94C for 3 minutes, followed by 28 cycles (5 cycle used on PCR products) of 94C for 30 seconds, 53C for 40 seconds and 72C for 1 minute, after which a final elongation step at 72C for 5 minutes was performed. Sequencing was performed at MR DNA (www.mrdnalab.com, Shallowater, TX, USA) on an Ion Torrent PGM following the manufacturers guidelines.
attributes.tag: BioSampleModel
attributes.value: MIMARKS.survey
attributes.tag: BioSampleModel
attributes.value: MIGS/MIMS/MIMARKS.wastewater