title: Sample 3
organism: Mus musculus
description: Protocols: Peyer's patches were harvested and washed with cold PBS. The tissue was digested using an enzyme mix comprised of RPMI containing 10% fetal bovine serum, 0.8 mg/ml Dispase, 0.1 mg/ml Collagenese P (Roche) and 0.1 mg/ml DNase I (Sigma) for 45 min at 37 °C. Cells were washed with cold PBS and suspended in FACS buffer (PBS supplemented with 0.5% bovine serum albumin (BSA) and 5 mM EDTA in PBS). Hematopoietic cells were depleted using anti-CD45 microbeads and magnetic based separation (Miltenyi). Remaining cells were stained with labelled antibodies and FRCs were sorted by FACS sorting. The cDNA library generation was performed following the established commercial protocol for Chromium Single Cell 3' Reagent Kit (v2 Chemistry or v3 Chemistry)
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2021-01-22T04:10:57Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-06-29T15:29:44Z
attributes.tag: External Id
attributes.value: SAMEA7025761
attributes.tag: INSDC center name
attributes.value: Institute of Immunobiology, Kantonsspital St. Gallen, St. Gallen, Switzerland
attributes.tag: INSDC first public
attributes.value: 2021-01-22T04:10:57Z
attributes.tag: INSDC last update
attributes.value: 2020-06-29T15:29:44Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-9162:Sample 3
attributes.tag: age
attributes.value: 8 to 10
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: fibroblast
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: C57BL/6N-Tg(Ccl19-Cre) x R26-EYFP
attributes.tag: individual
attributes.value: 3 mice pooled
attributes.tag: organism part
attributes.value: Peyer's patch
attributes.tag: sample name
attributes.value: E-MTAB-9162:Sample 3
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: mixed
attributes.tag: strain
attributes.value: C57BL/6NJ