title: Invertebrate sample from Rhodnius neglectus salivary glands
organism: Rhodnius neglectus
description: Rhodnius neglectus triatomines used in this work were reared in an insectary room at the University of Brasilia from insects collected in 1982 at Itambaraca, state of Parana, Brazil. They were kept at 27 C plus or minus 1 and relative humidity of 70-75%, and fed on Gallus gallus domesticus. The SGs used for transcriptome analysis were dissected from Vth instar nymphs and adults at 5, 12 and 24 days post blood meal and transferred immediately to cold Trizol reagent (Invitrogen, Carlsbad, CA, USA). The pool containing thirty SGs pairs was kept at -80 C prior RNA extraction.
attributes.tag: breed
attributes.value: Universidade de Brasilia colony
attributes.tag: isolation_source
attributes.value: Itambaraca
attributes.tag: collection_date
attributes.value: 1982
attributes.tag: geo_loc_name
attributes.value: Brazil:Itambaraca municipality, state of Parana
attributes.tag: tissue
attributes.value: salivary glands
attributes.tag: biomaterial_provider
attributes.value: Jaime Santana
attributes.tag: collected_by
attributes.value: Ionizete Garcia
attributes.tag: dev_stage
attributes.value: nymphs and adults
attributes.tag: sex
attributes.value: pooled male and female
attributes.tag: BioSampleModel
attributes.value: Invertebrate