title: SW480_39h
organism: Homo sapiens
description: Protocols: Cells were synchronised by medium change. Samples were collected starting 12 h after synchronisation in 3-h intervals for 30 h. Cells were maintained in DMEM low glucose supplemented with 10% FBS, 1x Penicillin/Streptomycin and 2 mM Ultraglutamine. Total RNA was extracted using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA) according to the manufacturer's protocol. mRNA libraries were prepared using the TruSeq Stranded mRNA Sample Preparation Kit (Illumina, San Diego, CA, USA) according to the low sample protocol guidelines.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-10-16T04:05:46Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-03-13T15:39:09Z
attributes.tag: External Id
attributes.value: SAMEA5418566
attributes.tag: INSDC center name
attributes.value: Charite - Universitatsmedizin Berlin, corporate member of Freie Universitat Berlin, Humboldt-Universitat zu Berlin, and Berlin Institute of Health
attributes.tag: INSDC first public
attributes.value: 2019-10-16T04:05:46Z
attributes.tag: INSDC last update
attributes.value: 2019-03-13T15:39:09Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7779:SW480_39h
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: SW480
attributes.tag: cell type
attributes.value: colorectal cancer cell
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: colon adenocarcinoma
attributes.tag: organism part
attributes.value: colon
attributes.tag: sample name
attributes.value: E-MTAB-7779:SW480_39h
attributes.tag: scientific_name
attributes.value: Homo sapiens