organism: seawater metagenome
attributes.tag: investigation_type
attributes.value: metagenome
attributes.tag: project_name
attributes.value: Near-Coral and Near-Seagrass Water Metagenomes from Dry Tortugas National Park
attributes.tag: collection_date
attributes.value: 2017-06-17
attributes.tag: env_package
attributes.value: mims extension
attributes.tag: env_biome
attributes.value: ocean
attributes.tag: env_feature
attributes.value: adjacent to seagrass
attributes.tag: env_material
attributes.value: water
attributes.tag: geo_loc_name
attributes.value: USA: Dry Tortugas National Park
attributes.tag: lat_lon
attributes.value: 24.70243 N 82.79868 W
attributes.tag: depth
attributes.value: 3.7-4.6 m
attributes.tag: samp_collect_device
attributes.value: Peristaltic pump
attributes.tag: samp_mat_process
attributes.value: Water (5L) was pumped through 0.8 um prefilters and 0.2 um Sterivex filters. Sterivex filters were then frozen in liquid nitrogen until DNA extraction within 14 days.
attributes.tag: nucl_acid_ext
attributes.value: Manufacturer's protocol for MO BIO PowerWater Sterivex DNA Isolation Kit; modified protocol to eliminate Steps 8 and 9 (heating and cooling steps)
attributes.tag: lib_const_meth
attributes.value: Nextera DNA Library Prep Kit
attributes.tag: seq_meth
attributes.value: Illumina HiSeq 4000 generating paired-end 150-bp reads
attributes.tag: BioSampleModel
attributes.value: MIMS.me
attributes.tag: BioSampleModel
attributes.value: MIGS/MIMS/MIMARKS.water