title: Ripe blueberry Lenoir cultivar
organism: Vaccinium corymbosum
description: Green or fully ripe blueberries were collected in 2009 from four or five year old southern highbush blueberry plants (cultivars Pamlico, Lenoir, O'Neal and Ozark Blue) growing at the North Carolina Department of Agriculture Piedmont Research Station near Salisbury, NC. Berries were frozen on liquid nitrogen in the field. RNA was extracted from whole berries using the Spectrum Total Plant RNA Kit (Sigma). Extracted RNAs were treated with DNAase I prior to library construction using RNAase Free DNase (catalog number 79254) from Qiagen. Libraries were synthesized using RNASeq Sample Preparation kit (catalog number RS-930-1001) from Illumina following manufacturer instructions. Libraries were sequenced using paired end, 76 cycle sequencing at the UNC Chapel Hill Lineberger Cancer Research Center on a GAIIx sequencer from Illumina. Visualizations of read sequence alignments onto a May 2013 release of a draft blueberry genome assembly sequence are available in Integrated Genome Browser. To obtain a copy of Integrated Genome Browser, visit http://www.bioviz.org. 
attributes.tag: cultivar
attributes.value: Lenoir
attributes.tag: biomaterial_provider
attributes.value: Ann Loraine
attributes.tag: tissue
attributes.value: berry
attributes.tag: age
attributes.value: 4
attributes.tag: collection_date
attributes.value: 2009
attributes.tag: dev_stage
attributes.value: ripe
attributes.tag: geo_loc_name
attributes.value: USA: North Carolina
attributes.tag: BioSampleModel
attributes.value: Plant