title: TARA_20131027T1016Z_210_EVENT_PUMP_SRF_(5m)_SEQ-(FeCl3)_W<0.22_R110001592
organism: marine metagenome
description: This sample (TARA_R110001592) was collected at station TARA_210 during campaign TARA_20131020Z of the Tara Oceans expedition (2009-2013), using a high volume peristaltic pump [HVP-PUMP]. The sampling event (TARA_20131027T1016Z_210_EVENT_PUMP) occurred at position latitudeN=61.5427 and longitudeE=-55.9869, on date/time=2013-10-27T10:16:00, at a depth of 3-7 m. The sample material was collected in the marine biome (ENVO:00000447) targeting a [SRF] surface water layer (ENVO:00010504). The sample material was size-fractionated (<0.22 micrometres) and prepared on board using protocol [SEQ-(FeCl3)_W<0.22] for later detection and identification of biological and molecular entities (nucleotides) by sequencing (SEQ) methods. This sample may be used for example in biodiversity and metagenomics studies of viruses. This sample has no known replicate.
attributes.tag: Chlorophyll Sensor
attributes.value: 0.99555
attributes.tag: Citation
attributes.value: Tara Oceans Consortium, Coordinators & Tara Oceans Expedition, Participants. Registry of all samples from the Tara Oceans Expedition (2009-2013). PANGAEA. doi:10.1594/PANGAEA.842197 (2015) (https://dx.doi.org/10.1594/PANGAEA.842197)
attributes.tag: ENA first public
attributes.value: 2018-08-29
attributes.tag: ENA last update
attributes.value: 2016-08-25
attributes.tag: Event Date/Time End
attributes.value: 2013-10-27T13:26:00
attributes.tag: External Id
attributes.value: SAMEA4398387
attributes.tag: INSDC center alias
attributes.value: GSC
attributes.tag: INSDC center name
attributes.value: Genoscope
attributes.tag: INSDC first public
attributes.value: 2018-08-29T17:02:51Z
attributes.tag: INSDC last update
attributes.value: 2016-08-25T12:24:56Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Latitude End
attributes.value: 61.5214
attributes.tag: Longitude End
attributes.value: -55.9973
attributes.tag: Salinity Sensor
attributes.value: 34.212612
attributes.tag: Submitter Id
attributes.value: TARA_R110001592
attributes.tag: depth
attributes.value: 5
attributes.tag: environment (biome)
attributes.value: Polar Biome
attributes.tag: environment (feature)
attributes.value: [SRF] surface water layer (ENVO:00010504)
attributes.tag: environment (feature) further details
attributes.value: discrete depth within the epipelagic zone (ENVO:00000209) at the top of the photic zone (ENVO:00000209), <50 m from a strong peak in chlorophyll fluorescence
attributes.tag: environment (material)
attributes.value: water (ENVO:00002006), including particulate matter (ENVO:01000060) such as planktonic material (ENVO:01000063)
attributes.tag: environmental package
attributes.value: water
attributes.tag: event date/time start
attributes.value: 2013-10-27T10:16:00
attributes.tag: event label
attributes.value: TARA_20131027T1016Z_210_EVENT_PUMP
attributes.tag: further details
attributes.value: http://store.pangaea.de/Projects/TARA-OCEANS/Logsheets_Wetlab/TARA_20131027Z_210_WETLAB_VIRUS.pdf
attributes.tag: last update date
attributes.value: 2016-04-23Z
attributes.tag: latitude start
attributes.value: 61.5427
attributes.tag: longitude start
attributes.value: -55.9869
attributes.tag: marine region
attributes.value: [AO] Arctic Ocean (MRGID:1906)
attributes.tag: nitrate sensor
attributes.value: 8.291587
attributes.tag: oxygen sensor
attributes.value: 293.88825
attributes.tag: project name
attributes.value: Tara Oceans Expedition (2009-2013)
attributes.tag: protocol description
attributes.value: In the size fraction W<0.22 micrometres, the sample material was pre-filtered using a 142-mm-diameter Millipore polyethersulfone Express Plus membrane (ref:GPWP14250) with 0.22-micrometres-pore-size. A volume of 20 Litres was treated with FeCl3, incubated at room temperature for 1-24 hours to allow viruses to precipitate, filtered onto a 1-micrometre-pore-size, 142-mm-diameter GE polycarbonate (PC) membrane filter (ref:K10CP14220) placed on top of a 0.8-micrometre-pore-size, 142-mm-diameter Pall Gelman support filter (ref:60114) using a peristaltic pump with pressure <15 psi. The 1-micrometre-pore-size filter was then packaged in a sterile Falcon tube (50 mL) (up to 3 filters in 1 tube), labelled with a barcode identification sticker and stored on board in a refrigerator at +4 degree Celsius. Note 1: Latex or nitril gloves were used for this protocol. Note 2: All containers, filter holders and tubing were washed with 0.1% bleach, rinsed twice with fresh water and rinsed again twice with the pre-filtered sample material. All tweezers are kept clean with ethanol. Note 3: The three replicates were incubated together. For 20 Litres of filtrate, 2 millilitres of FeCl3 stock solution (4.83 grams of FeCl3-6H2O + 100 millilitres of MilliQ water, stored at 4°C) was added (final concentration of 1 mg Fe per Litre of filtrate), and shaken vigorously for 1 minute. Note 4: Further details about the method are published here doi:10.1111/j.1758-2229.2010.00208.x. In 2009-2012, a total of 3 replicates were prepared and sent for later analysis to the University of Arizona (UA), Tucson, USA. In 2013, a total of 2 replicates were prepared, one sent to for later analysis to the University of Arizona (UA), Tucson, USA, and the other sent for later analysis to the Observatoire Océanologique de Banyuls sur Mer (OOB), France.
attributes.tag: protocol label
attributes.value: SEQ-(FeCl3)_W<0.22
attributes.tag: sample collection device
attributes.value: high volume peristaltic pump [HVP-PUMP]
attributes.tag: sample material label
attributes.value: TARA_210_SRF_<-0.22
attributes.tag: sample name
attributes.value: TARA_R110001592
attributes.tag: sample status
attributes.value: This version can be used to provide data discovery services
attributes.tag: sampling campaign
attributes.value: TARA_20131020Z
attributes.tag: sampling platform
attributes.value: SV Tara
attributes.tag: sampling station
attributes.value: TARA_210
attributes.tag: size fraction lower threshold
attributes.value: <
attributes.tag: size fraction upper threshold
attributes.value: 0.22
attributes.tag: temperature
attributes.value: 5.2939