title: NAII_HEKwt_dN_1
organism: Homo sapiens
description: Protocols: HEK 293 or HeLa cells were seeded and transfected with the indicated constructs for indicated time points. Genomic DNA was isolated using the DNeasy Blood & Tissue Kit (QIAGEN) according to the manufacturers instructions. Repaired plasmids and genomic junctions were PCR-amplified using specific primers with different 6bp 5' tags. For genomic amplicons, the Nextera XT Index Kit (Illumina) was used. Amplicons were pooled and sequenced on the Illumina MiSeq platform.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2021-01-20T04:11:28Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-08-11T11:54:04Z
attributes.tag: External Id
attributes.value: SAMEA7175924
attributes.tag: INSDC center name
attributes.value: 1 Department of Internal Medicine III with Haematology , Medical Oncology, Haemostaseology, Infectiology and Rheumatology, Oncologic Center, Salzburg Cancer Research Institute Laboratory for Immunological and Molecular Cancer Research (SCRI LIMCR), Paracelsus Medical University, Salzburg, Austria, Cancer Cluster Salzburg, Austria 2 Department of Biosciences, Paris Lodron University of Salzburg, Austria
attributes.tag: INSDC first public
attributes.value: 2021-01-20T04:11:28Z
attributes.tag: INSDC last update
attributes.value: 2020-08-11T11:54:04Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-83821597143176:NAII_HEKwt_dN_1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: HEK293
attributes.tag: cell type
attributes.value: epithelial cell
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: embryo
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: kidney
attributes.tag: sample name
attributes.value: E-MTAB-83821597143176:NAII_HEKwt_dN_1
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: transfection
attributes.value: 4 nCas9 constructs