title: HeLaS3_rep1
organism: Homo sapiens
description: Protocols: HeLaS3 cells were grown in DMEM supplemented with 10%FBS, pen/strep and L-glutamine. HeLaS3 cells were transfected with 10nMol/l siRNA using Interferin reagent (Polyplus) according to manufacturer instructions. Total RNA was extracted using Tri-reagent according to manufacturers protocol (Sigma). mRNA libraries were constructed using the TruSeq RNA Sample Preparation Kits v2 (Illumina) starting from total RNA
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2014-03-03T16:32:10Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T16:59:15Z
attributes.tag: External Id
attributes.value: SAMEA2267175
attributes.tag: INSDC center name
attributes.value: Sapienza Universita di Roma
attributes.tag: INSDC first public
attributes.value: 2014-03-03T16:32:10Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T16:59:15Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-2128:HeLaS3_rep1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: HeLa-S3
attributes.tag: common name
attributes.value: human
attributes.tag: sample name
attributes.value: E-MTAB-2128:HeLaS3_rep1
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: specimen with known storage state
attributes.value: frozen specimen