title: P3 ripe
organism: Vaccinium corymbosum
description: Berries at varying stages were selected from three plants (3-33, 2-41, and 2-42) of the O'Neal variety of southern highbush blueberry Vaccinium corymbosum. The five stages were designated as pads, cups, green, pink, and ripe. Stages pads and cups corresponded approximately to stages S1/S2 (pads) and S3/S4 (cups) described in (Zifkin et al., 2012 PMID 22086422). Green fruit were fully rounded green berries, pink berries were partially pigmented but still firm, and ripe berries were fully colored and soft. Samples were collected during the growing season of 2011. Pads were collected on April 4, cups on April 19, mature green fruit on April 28, pink fruit on May 20, and ripe fruit on June 2. Following RNA extraction and DNAase I treatment, libraries were synthesized using the TruSeq A kit (catalog number FC-121-1001) from Illumina following the manufacturerÕs instructions. Libraries were synthesized using different TruSeq adapters to allow multiplexing, combined and then sequenced in three lanes with five libraries per lane using a HiSeq sequencer from Illumina. One library (3-33 pads) was sequenced in two lanes.
attributes.tag: cultivar
attributes.value: O'Neal
attributes.tag: biomaterial_provider
attributes.value: April Estrada
attributes.tag: tissue
attributes.value: berry
attributes.tag: collected_by
attributes.value: April Estrada
attributes.tag: collection_date
attributes.value: 6/2/11
attributes.tag: dev_stage
attributes.value: ripe
attributes.tag: growth_protocol
attributes.value: field
attributes.tag: sample_id
attributes.value: 10
attributes.tag: geo_loc_name
attributes.value: missing
attributes.tag: BioSampleModel
attributes.value: Plant