organism: Homo sapiens
description: Protocols: Fetuses collected by trained nurses and processed immediately. Fetal weight, morphology, sex, crown-rump length, ano-genital distance and supine distance were recorded. Blood samples were collected by inserting a needle into the chest cavity towards the heart, under the ribs. The abdomen of the fetus was entered using scissors and tweezers to cut down the midline of the abdomen and under the rib cage to expose the internal organs. The liver was removed, placed onto autoclaved lint-free paper to remove excess connective tissue and blood. The liver was weighed. Three 30 mg pieces of liver were cut from the main lobe and snap frozen in separate tubes. A larger piece from the main lobe was fixed in NBF for sectioning. The remaining liver was snap frozen in a small plastic sleeve. RNA was extracted from the liver samples using the Qiagen AllPrep DNA/RNA/Protein Mini kit as per manufacturer's instructions. Illumina TruSeq Stranded mRNA Library Prep Kit
attributes.tag: ENA first public
attributes.value: 2018-09-01
attributes.tag: ENA last update
attributes.value: 2017-09-29
attributes.tag: External Id
attributes.value: SAMEA104326063
attributes.tag: INSDC center alias
attributes.value: Centre for Genome Enabled Biology and Medicine University of Aberdeen
attributes.tag: INSDC center name
attributes.value: Centre for Genome Enabled Biology and Medicine University of Aberdeen
attributes.tag: INSDC first public
attributes.value: 2018-09-01T17:02:07Z
attributes.tag: INSDC last update
attributes.value: 2017-09-29T14:25:05Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6103:387
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: not available
attributes.tag: disease
attributes.value: normal
attributes.tag: organism part
attributes.value: liver
attributes.tag: sample name
attributes.value: E-MTAB-6103:387
attributes.tag: sex
attributes.value: female