organism: Mus musculus
description: Protocols: After dissociation cells were sorted for Gata6::mCherry or Nanog::GFP expression (SL grown cells). 2iLIF cells were sent through the machine without actual sorting. Total RNA was collected Cells grown in 2iLIF on gelatin coated plates and transferred to SerumLIF medium on gelatin for 86h Mouse embryonic stem cells, transfected with gRNAs CACACTCAAGGATCCACAGG and GCCACAGATGGTGTCCGCGG, Dax1 mutant clone RNeasy Mini Kit with on-column DNase digest (Qiagen) TruSeq Illumina mRNA-seq (stranded - high input)
attributes.tag: ENA first public
attributes.value: 2021-03-23
attributes.tag: ENA last update
attributes.value: 2021-02-25
attributes.tag: External Id
attributes.value: SAMEA8138918
attributes.tag: INSDC center alias
attributes.value: Friedrich Miescher Institute for Biomedical Research
attributes.tag: INSDC center name
attributes.value: Friedrich Miescher Institute for Biomedical Research
attributes.tag: INSDC first public
attributes.value: 2021-03-23T08:57:12Z
attributes.tag: INSDC last update
attributes.value: 2021-02-25T17:24:17Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-10150:D8_G
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: TNG-A
attributes.tag: cell type
attributes.value: mouse embryonic stem cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: mouse embryo stage
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: DAX1 -/-
attributes.tag: growth condition
attributes.value: SL
attributes.tag: phenotype
attributes.value: GFP+ mCherry-
attributes.tag: sample name
attributes.value: E-MTAB-10150:D8_G