organism: Anopheles arabiensis
description: Adult An. arabiensis mosquitoes were homogenized with liquid nitrogen in a precooled mortar. Then, mosquitoes were fixed with 3% formaldehyde. MboI restriction enzyme (NEB, #R0147) with average restriction fragment size ~250-300 bp was used in the experiment. Two biological replicates of Hi-C libraries were generated. One replicate had 30 males and 30 females. The other replicate had 15 males and 15 females. There libraries were prepared with NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, #E7103), and sequenced using 150-bp pair-ended sequencing on Illumina platform.
attributes.tag: breed
attributes.value: Dongola
attributes.tag: isolation_source
attributes.value: Laboratory
attributes.tag: collection_date
attributes.value: 2019-03-12
attributes.tag: geo_loc_name
attributes.value: Sudan: Dongola
attributes.tag: tissue
attributes.value: not applicable
attributes.tag: sex
attributes.value: pooled male and female
attributes.tag: BioSampleModel
attributes.value: Invertebrate