organism: Magallana gigas
description: Pacific oyster (Crassostrea gigas) hemolymph microbiota; collected on Sylt, 2012-06-21; sample taken after field deployment; not antibiotic treated; oyster from Sylt, not translocated
attributes.tag: ENA first public
attributes.value: 2016-06-29
attributes.tag: ENA last update
attributes.value: 2016-10-21
attributes.tag: External Id
attributes.value: SAMEA3461848
attributes.tag: INSDC center alias
attributes.value: ALFRED WEGENER INSTITUTE FOR POLAR AND MARINE RESE
attributes.tag: INSDC center name
attributes.value: ALFRED WEGENER INSTITUTE FOR POLAR AND MARINE RESE
attributes.tag: INSDC first public
attributes.value: 2016-06-29T17:01:56Z
attributes.tag: INSDC last update
attributes.value: 2016-10-21T09:39:27Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: Cg_159_Hem_Sy_SyC_20120621
attributes.tag: amount or size of sample collected
attributes.value: 0.002
attributes.tag: collection date
attributes.value: 2012-06-21
attributes.tag: common name
attributes.value: Pacific oyster
attributes.tag: environment (biome)
attributes.value: aquatic, marine, benthic, coastal
attributes.tag: environment (feature)
attributes.value: organic
attributes.tag: environment (material)
attributes.value: organic material, bodily fluid
attributes.tag: geographic location (country and/or sea)
attributes.value: North Sea
attributes.tag: geographic location (latitude)
attributes.value: 55.0294213
attributes.tag: geographic location (longitude)
attributes.value: 8.43423032
attributes.tag: geographic location (region and locality)
attributes.value: Schleswig Holstein, Sylt, List, Odewatt
attributes.tag: host body habitat
attributes.value: hemolymph
attributes.tag: host body site
attributes.value: adductor muscle
attributes.tag: host common name
attributes.value: Pacific oyster
attributes.tag: host life stage
attributes.value: adult
attributes.tag: host subject id
attributes.value: Sy_159
attributes.tag: host taxid
attributes.value: 29159
attributes.tag: host-associated environmental package
attributes.value: host-associated
attributes.tag: investigation type
attributes.value: mimarks-survey
attributes.tag: multiplex identifiers
attributes.value: f: CGTACGTA; r: CGTTCCTA
attributes.tag: pcr conditions
attributes.value: initial denaturation:98degC_30s; denaturation:98degC_9s;annealing:55degC_1min; elongation:72degC_90s; final elongation:72degC_10min; 30 cycles
attributes.tag: pcr primers
attributes.value: 27f-338r
attributes.tag: pcr primers
attributes.value: 27F: TCAGAGTTTGATCMTGGCTCAG + 338r: CATGCTGCCTCCCGTAGGAGT
attributes.tag: perturbation
attributes.value: not antibiotic treated
attributes.tag: project name
attributes.value: PRJEB9624
attributes.tag: sample collection device or method
attributes.value: syringe
attributes.tag: sample name
attributes.value: Cg_159_Hem_Sy_SyC_20120621
attributes.tag: sample storage temperature
attributes.value: -80
attributes.tag: sample volume or weight for DNA extraction
attributes.value: 0.2
attributes.tag: sequencing method
attributes.value: Sequencing by synthesis, Illumina paired-end
attributes.tag: target gene
attributes.value: 16s rRNA
attributes.tag: target subfragment
attributes.value: V1-V2
attributes.tag: temperature
attributes.value: 15.092