organism: Mus musculus
description: Protocols: Mice were treated acutely with an intraperitoneal injection of dexamethasone or vehicle, and liver tissue harvested two hours later. RNA extraction from flash-frozen mouse liver tissue was performed using the ReliaPrep RNA Miniprep system (Promega), as per manufacturer's instructions, incorporating a DNase treatment step. Lysing Matrix D tubes (MP Biomedicals) were used to homogenise tissue. Illumina TruSeq Stranded mRNA kit, according to the manufacturer's protocol.
attributes.tag: ENA first public
attributes.value: 2021-04-10
attributes.tag: ENA last update
attributes.value: 2021-03-22
attributes.tag: External Id
attributes.value: SAMEA8391966
attributes.tag: INSDC center alias
attributes.value: UNIVERSITY OF MANCHESTER
attributes.tag: INSDC center name
attributes.value: UNIVERSITY OF MANCHESTER
attributes.tag: INSDC first public
attributes.value: 2021-04-10T00:31:42Z
attributes.tag: INSDC last update
attributes.value: 2021-03-22T10:56:37Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-10247:Hnf4_CreNeg_DEX_rep2
attributes.tag: age
attributes.value: 6 to 8
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: young adult
attributes.tag: genotype
attributes.value: Hnf4aFlox AlbCre-/-
attributes.tag: organism part
attributes.value: liver
attributes.tag: sample name
attributes.value: E-MTAB-10247:Hnf4_CreNeg_DEX_rep2
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: C57BL/6