organism: seawater metagenome
description: seawater sample taken near the oyster reef at low tide; temperature and salinity measured at a nearby site by ship Mya IIl
attributes.tag: ENA first public
attributes.value: 2016-06-29
attributes.tag: ENA last update
attributes.value: 2016-10-21
attributes.tag: External Id
attributes.value: SAMEA3462823
attributes.tag: INSDC center alias
attributes.value: ALFRED WEGENER INSTITUTE FOR POLAR AND MARINE RESE
attributes.tag: INSDC center name
attributes.value: ALFRED WEGENER INSTITUTE FOR POLAR AND MARINE RESE
attributes.tag: INSDC first public
attributes.value: 2016-06-29T17:01:55Z
attributes.tag: INSDC last update
attributes.value: 2016-10-21T09:44:06Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: SW_Texel_20120727
attributes.tag: collection date
attributes.value: 2012-07-27
attributes.tag: environment (biome)
attributes.value: aquatic, marine, benthic, coastal
attributes.tag: environment (feature)
attributes.value: oyster reef
attributes.tag: environment (material)
attributes.value: environmental material, water, seawater
attributes.tag: geographic location (country and/or sea)
attributes.value: North Sea
attributes.tag: geographic location (depth)
attributes.value: 0.5
attributes.tag: geographic location (latitude)
attributes.value: 53.00167
attributes.tag: geographic location (longitude)
attributes.value: 4.789167
attributes.tag: investigation type
attributes.value: mimarks-survey
attributes.tag: multiplex identifiers
attributes.value: f: CTCTTCTC; r: GCATTAGC
attributes.tag: pcr conditions
attributes.value: initial denaturation:98degC_30s; denaturation:98degC_9s;annealing:55degC_1min; elongation:72degC_90s; final elongation:72degC_10min; 30 cycles
attributes.tag: pcr primers
attributes.value: 27f-338r
attributes.tag: pcr primers
attributes.value: 27F: TCAGAGTTTGATCMTGGCTCAG + 338r: CATGCTGCCTCCCGTAGGAGT
attributes.tag: project name
attributes.value: PRJEB9624
attributes.tag: salinity
attributes.value: 27.23441322
attributes.tag: sample name
attributes.value: SW_Texel_20120727
attributes.tag: sample volume or weight for DNA extraction
attributes.value: 100
attributes.tag: sequencing method
attributes.value: Sequencing by synthesis, Illumina paired-end
attributes.tag: target gene
attributes.value: 16s rRNA
attributes.tag: target subfragment
attributes.value: V1-V2
attributes.tag: temperature
attributes.value: 19.67009995
attributes.tag: water environmental package
attributes.value: water