organism: Mus musculus
description: Protocols: Spleens were collected from mice between 6-12 weeks old Untouched naive cells were purified from the spleen with negative selection protocol. Spleens were mashed through a 70nm strainer to obtain single cell suspension Cells were grown in complete IMDM 10% FBS, in presence of IL4 (10ng/ml) Libraries were prepared according to Illumina Paired End sequencing Protocl
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2016-04-01T17:05:10Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-09T00:28:31Z
attributes.tag: External Id
attributes.value: SAMEA3375182
attributes.tag: INSDC center name
attributes.value: EMBL-EBI
attributes.tag: INSDC first public
attributes.value: 2016-04-01T17:05:10Z
attributes.tag: INSDC last update
attributes.value: 2018-03-09T00:28:31Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-3543:GEN0N_B
attributes.tag: age
attributes.value: 6-12 weeks
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: naive T cells activated (with anti-CD3/CD28) and polarized towards the Th2 subtype with IL4
attributes.tag: common name
attributes.value: house mouse
attributes.tag: differentiated
attributes.value: negative marker
attributes.tag: generation
attributes.value: 0
attributes.tag: sample name
attributes.value: E-MTAB-3543:GEN0N_B
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: stimulus
attributes.value: anti-CD3/CD28 and polarized towards the Th2 subtype with IL4