organism: Homo sapiens
description: Protocols: The cells were generated by infecting cells with 2nd generation lentiviruses. The genes are under Tet-on control. 2d and 4d indicates either doxycycline induction for 2 or 4 days. Het1A, Het1A-HNF4A and Het1A-GATA6 cells were cultured in DMEM (+10% FBS and 1% PenStrep) with 100ng/mL doxycycline for 2 or 4 days. Cells were trypsinised and counted. 50000 cells were taken forward for Tn5 reaction. 50000 cells were lysed in ATAC lysis buffer and Tn5 reactions were performed as described in Transposition of native chromatin for fast and sensitive epigenomic profiling of open chromatin, DNA-binding proteins and nucleosome position. 2013. Jason D. Buenrostro, Paul G. Giresi, Lisa C. Zaba, Howard Y. Chang and William J. Greenleaf. doi:10.1038/nmeth.2688 Libraries were constructed by amplifying transposed DNA with Illumina Nextera adapters.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-08-30T17:02:49Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-06-22T14:00:08Z
attributes.tag: External Id
attributes.value: SAMEA4753717
attributes.tag: INSDC center name
attributes.value: UOM
attributes.tag: INSDC first public
attributes.value: 2018-08-30T17:02:49Z
attributes.tag: INSDC last update
attributes.value: 2018-06-22T14:00:08Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6931:Het1A_+dox_rep1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: Het1A
attributes.tag: cell type
attributes.value: epithelial cell
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: esophagus
attributes.tag: phenotype
attributes.value: wild type phenotype
attributes.tag: sample name
attributes.value: E-MTAB-6931:Het1A_+dox_rep1
attributes.tag: scientific_name
attributes.value: Homo sapiens