organism: Equus caballus
description: Protocols: Cells were cultured for 24 h prior to a 4 h treatment with vehicle (DMSO) or DMBA. Total RNA was extracted with TRIzol reagent following the recommendations of the manufacturer. The quality of total RNA was evaluated using the Agilent 2100 bioanalyzer (Agilent, Palo Alto, CA, USA) with the RNA 6000 Nano LabChip kit. RNA-seq libraries were prepared with the NEBNext Ultra Directional RNA Library Prep Kit (New England Biolabs) using 500 ng total RNA followed by polyA+ enrichment.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-08-31T17:03:39Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-08-01T09:41:51Z
attributes.tag: External Id
attributes.value: SAMEA4810955
attributes.tag: INSDC center name
attributes.value: Baker Institute, Cornell University
attributes.tag: INSDC first public
attributes.value: 2018-08-31T17:03:39Z
attributes.tag: INSDC last update
attributes.value: 2018-08-01T09:41:51Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7103:HB-DMSO
attributes.tag: breed
attributes.value: Thoroughbred
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: mammary stem cell
attributes.tag: common name
attributes.value: horse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: individual
attributes.value: 4
attributes.tag: organism part
attributes.value: mammary gland
attributes.tag: sample name
attributes.value: E-MTAB-7103:HB-DMSO
attributes.tag: scientific_name
attributes.value: Equus caballus
attributes.tag: sex
attributes.value: female