organism: Herbaspirillum seropedicae SmR1
description: Protocols: Herbaspirillum seropedicae SmR1 was grown in NFbHPN medium until OD600nm of 0.6, when 5mM phenol or 2,5mM benzoic acid was added to the medium. After 30 minutes of growth, the cells were collected by centrifugation and total RNA was extracted with RiboPureBacteria. RiboPure Bacteria (Ambion) After rRNA depletion 500 ng of depleted rRNA was used to construct the sequencing libraries following standard protocols using the SOLiD Total RNA-Seq Kit (Life Technologies). The libraries were barcoded by using the SOLiD Transcriptome Multiplexing Kit (Life Technologies).
attributes.tag: ENA first public
attributes.value: 2016-04-01
attributes.tag: ENA last update
attributes.value: 2015-04-07
attributes.tag: External Id
attributes.value: SAMEA3333541
attributes.tag: INSDC center alias
attributes.value: UFPR
attributes.tag: INSDC center name
attributes.value: UFPR
attributes.tag: INSDC first public
attributes.value: 2016-04-01T17:05:10Z
attributes.tag: INSDC last update
attributes.value: 2015-04-07T14:55:27Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-3467:SmR1 benzoic acid B
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: growth condition
attributes.value: NFbHPN + 2.5mM benzoic acid
attributes.tag: sample name
attributes.value: E-MTAB-3467:SmR1 benzoic acid B