organism: Herbaspirillum seropedicae SmR1
description: Protocols: Herbaspirillum seropedicae RP (derivative strain resistant to 1mM phenol) was grown in NFbHPN medium containing 1mM phenol until OD600nm of 0.6, then 5mM phenol was added to the medium. After 30 minutes of growth, the cells were collected by centrifugation and total RNA was extracted with RiboPureBacteria. RiboPure Bacteria (Ambion) After rRNA depletion 500 ng of depleted rRNA was used to construct the sequencing libraries following standard protocols using the SOLiD Total RNA-Seq Kit (Life Technologies). The libraries were barcoded by using the SOLiD Transcriptome Multiplexing Kit (Life Technologies).
attributes.tag: ENA first public
attributes.value: 2016-04-01
attributes.tag: ENA last update
attributes.value: 2015-04-07
attributes.tag: External Id
attributes.value: SAMEA3333535
attributes.tag: INSDC center alias
attributes.value: UFPR
attributes.tag: INSDC center name
attributes.value: UFPR
attributes.tag: INSDC first public
attributes.value: 2016-04-01T17:05:10Z
attributes.tag: INSDC last update
attributes.value: 2015-04-07T14:55:27Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-3467:RP control
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: growth condition
attributes.value: NFbHPN 1mM phenol
attributes.tag: sample name
attributes.value: E-MTAB-3467:RP control