organism: Mus musculus
description: Protocols: Grem1 positive and negative FRCs (between 30,000 to 1,00,000 cells) (CD45- Ter119- MADCAM1- CD21/35- CD31- PDPN+) from skin draining lymph nodes of Grem1-Cre-ERT2 .cki; Rosa26-LSL-EYFP mice were sorted by FACS. The samples were directly sorted into TRIZOL (Invitrogen) and RNA was isolated at Expression Analysis, Inc. Paired-end RNA-seq libraries were constructed using SMARTer Low Input RNA Kit (Takara).
attributes.tag: ENA first public
attributes.value: 2021-04-10
attributes.tag: ENA last update
attributes.value: 2021-03-11
attributes.tag: External Id
attributes.value: SAMEA8234992
attributes.tag: INSDC center alias
attributes.value: GENENTECH
attributes.tag: INSDC center name
attributes.value: GENENTECH
attributes.tag: INSDC first public
attributes.value: 2021-04-10T00:31:40Z
attributes.tag: INSDC last update
attributes.value: 2021-03-11T16:54:04Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-10205:Grem1+ R1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: reticular cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: Grem1-Cre-ERT2.cki; Rosa26-LSL-EYFP
attributes.tag: immunophenotype
attributes.value: CD45-; Ter119-MADCAM1-; CD21/35-; CD31-; PDPN+
attributes.tag: individual
attributes.value: 1
attributes.tag: organism part
attributes.value: mixed pool of axillary lymph node; brachial lymph node; inguinal lymph node
attributes.tag: phenotype
attributes.value: Grem1 positive
attributes.tag: sample name
attributes.value: E-MTAB-10205:Grem1+ R1
attributes.tag: strain
attributes.value: C57BL6/J