organism: Mus musculus
description: Protocols: After dissociation cells were counted by FACS (no sorting). Cell concentration was determined using a TC20 automated cell counter (BioRad) Cells grown in 2iLIF on gelatin coated plates and transferred to SerumLIF medium on gelatin for 86h Cells for the samples were loaded into channel of a Chromium Single Cell A Chip (10X genomics) channels. Reverse-transcription, cDNA pre-amplification (13 cycles) and library preparation were performed according to the manufacturer instructions (Chromium Single Cell 3′ Library & Gel Bead Kit v3, 10X genomics).
attributes.tag: ENA first public
attributes.value: 2021-03-23
attributes.tag: ENA last update
attributes.value: 2021-02-25
attributes.tag: External Id
attributes.value: SAMEA8138937
attributes.tag: INSDC center alias
attributes.value: Friedrich Miescher Institute for Biomedical Research
attributes.tag: INSDC center name
attributes.value: Friedrich Miescher Institute for Biomedical Research
attributes.tag: INSDC first public
attributes.value: 2021-03-23T08:57:12Z
attributes.tag: INSDC last update
attributes.value: 2021-02-25T17:30:34Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-10147:WT
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: TNG-A (WT1 parental)
attributes.tag: cell type
attributes.value: mouse embryonic stem cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: mouse embryo stage
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: sample name
attributes.value: E-MTAB-10147:WT