organism: Homo sapiens
description: Protocols: HR1 cells were reverse-transfected with 5nM siRNA targeting hnRNPC mRNA (HSS179304 and HSS179305) or siRNA targeting Upf1 mRNA (HSS109172) as well as control siRNA Negative Control with medium GC content (Invitrogen). Depletion of hnRNPC was achieved 48 hours after initial siRNA transfection. For depletion of Upf1, cells were transfected a second time after 48 hours, and collected 96 hours after the first siRNA transfection. RNA was extracted using trizol as per manufacturer instructions. RNAseq libraries were produced using TruSeq stranded mRNAseq library kit (Illumina).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2016-04-01T17:05:18Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-09T09:24:54Z
attributes.tag: External Id
attributes.value: SAMEA3639216
attributes.tag: INSDC center name
attributes.value: Department of Molecular Neuroscience, UCL Institute of Neurology
attributes.tag: INSDC first public
attributes.value: 2016-04-01T17:05:18Z
attributes.tag: INSDC last update
attributes.value: 2018-03-09T09:24:54Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-4008:Sample 8
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: HEK293
attributes.tag: common name
attributes.value: human
attributes.tag: rna interference
attributes.value: siRNA hnRNPC #2
attributes.tag: sample name
attributes.value: E-MTAB-4008:Sample 8
attributes.tag: scientific_name
attributes.value: Homo sapiens