organism: Mus musculus
description: Protocols: Ctnnb1fl/fl mice were crossed with Rosa26-CreER Rosa26-YFP mice to generate Ctnnb1fl/fl RosaCre-ER RosaYFP mice. These mice were crossed with Hoxa9-/- knockout mice to generate Hoxa9-/- Ctnnb1fl/fl RosaCre-ER RosaYFP mice. Compound homozygous animals were used for experiments. For leukemia development experiments, C57BL/6 or SJL mice were given 11Gy total body Îł-irradiation and injected with up to 500,000 test cells mixed with 200,000 C57BL/6 or SJL bone marrow nuclear cells. LSK (lin-, Sca-1+, c-Kit+) and GMP (lin-, Sca-1-, c-Kit+, CD34+, CD16/32lo) were isolated from lineage negative cells after lineage (Gr-1, Mac-1, B220, Ter119, CD3e, CD4, CD8) depletion using a BD FACS ARIA cell sorter. 300ng to 1ug of mIRVANA (Ambion) isolated total RNA was used for RNA-Seq library preparation using TruSeq Stranded Total RNA kit (Illumina).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2016-06-30T17:01:22Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-09T01:55:58Z
attributes.tag: External Id
attributes.value: SAMEA3460518
attributes.tag: INSDC center name
attributes.value: 'Eric So lab Leukaemia and Stem Cell Biology Group Department of Haematological Medicine King''s College London'
attributes.tag: INSDC first public
attributes.value: 2016-06-30T17:01:22Z
attributes.tag: INSDC last update
attributes.value: 2018-03-09T01:55:58Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-3647:GMP_WT_MLL-ENL_3_F
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: granulocyte monocyte progenitor cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: disease
attributes.value: acute myeloid leukemia
attributes.tag: genotype
attributes.value: floxed
attributes.tag: individual
attributes.value: 60
attributes.tag: sample name
attributes.value: E-MTAB-3647:GMP_WT_MLL-ENL_3_F
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: transfection
attributes.value: MLL-ENL