organism: Mus musculus
description: Protocols: total RNA collected Cells grown in 2iLIF on gelatin coated plates and transferred to SerumLIF 1nM Retinoic Acid on gelatin for 48h G6C18 mouse embryonic stem cells, transfected with gRNAs CACACTCAAGGATCCACAGG and GCCACAGATGGTGTCCGCGG, mutant clone RNeasy Mini Kit with on-column DNase digest (Qiagen) TruSeq Illumina mRNA-seq (stranded - high input)
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2021-03-23T08:56:58Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-08-19T12:32:44Z
attributes.tag: External Id
attributes.value: SAMEA7195722
attributes.tag: INSDC center name
attributes.value: Friedrich Miescher Institute for Biomedical Research
attributes.tag: INSDC first public
attributes.value: 2021-03-23T08:56:58Z
attributes.tag: INSDC last update
attributes.value: 2020-08-19T12:32:44Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-9447:dax1_cl12_SL
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: TNG-A:G6C18
attributes.tag: cell type
attributes.value: mouse embryonic stem cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: embryo stage
attributes.tag: genetic modification
attributes.value: gene knock out
attributes.tag: genotype
attributes.value: dax1 -/-
attributes.tag: growth condition
attributes.value: SL
attributes.tag: sample name
attributes.value: E-MTAB-9447:dax1_cl12_SL
attributes.tag: scientific_name
attributes.value: Mus musculus