organism: Homo sapiens
description: Protocols: Samples were directly collected in TRIZOL RNA was extracted by TRIZOL. Then, 15 µg of RNA were incubated with 1 µl of DNAse (RQ1 RNase-Free DNase, M6101 Promega) for 30 min at 37°C with the indicated buffer and RNA was cleaned up using the Qiagen RNeasy kit (Qiagen #74104). Purity and concentrations were determined with an Agilent 2100 bioanalyzer and 1 µg was sent for RNAseq analysis (after polyA selection) to Beckman Coulter Genomics. polyA selection, RNA library preparation and multiplexing was performed at Genewiz
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2021-03-23T08:54:45Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-09-25T18:02:33Z
attributes.tag: External Id
attributes.value: SAMEA7340282
attributes.tag: INSDC center name
attributes.value: UCLouvain, de Duve Institute & WELBIO
attributes.tag: INSDC first public
attributes.value: 2021-03-23T08:54:45Z
attributes.tag: INSDC last update
attributes.value: 2020-09-25T18:02:33Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-9627:SZRD1 overexpression 2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: HeLa
attributes.tag: common name
attributes.value: human
attributes.tag: genotype
attributes.value: SZRD1 overexpression
attributes.tag: sample name
attributes.value: E-MTAB-9627:SZRD1 overexpression 2
attributes.tag: scientific_name
attributes.value: Homo sapiens