organism: Zaire ebolavirus
description: Protocols: Samples were acquired from BEI Resources (www.beiresources.org). RNA extracted from infected cell culture BEI Resources exrtacted RNA with TriPure Isolation Reagent (Roche Applied Science) RNA samples were processed following the protocol for the Illumina TruSeq Stranded mRNA Sample Preparation Kit. One microgram of total RNA was fragmented and reverse-transcribed into cDNAs [the poly(A)-tailed RNA enrichment was skipped, as viral RNA may not contain poly(A) tails]. Double-strand cDNAs were adenylated at the 3вЂ™ ends and individually indexed, followed by limited-cycle (15) amplification
attributes.tag: ENA first public
attributes.value: 2021-03-03
attributes.tag: ENA last update
attributes.value: 2021-02-15
attributes.tag: External Id
attributes.value: SAMEA8070906
attributes.tag: INSDC center alias
attributes.value: US Food and Drug Administration, Center for Biologics Evaluation and Research
attributes.tag: INSDC center name
attributes.value: US Food and Drug Administration, Center for Biologics Evaluation and Research
attributes.tag: INSDC first public
attributes.value: 2021-03-03T04:15:35Z
attributes.tag: INSDC last update
attributes.value: 2021-02-15T14:01:39Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-10102:Zaire Ebolavirus (Mayinga)
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: whole organism
attributes.tag: sample name
attributes.value: E-MTAB-10102:Zaire Ebolavirus (Mayinga)
attributes.tag: strain
attributes.value: Mayinga