organism: Homo sapiens
description: Protocols: Following compound treatment and UV irradiation, cells were directly lysed in dishes with Ribozol reagent. All samples were subject to RNA capture/pulldown using Desthiobiotin-Streptavidin. Direct-zol RNA purification kit (Zymo Research) Total RNA was subjected to fragmentation, conjugation of desthiobiotin using \click\ chemistry, ligation of a 3'-adapter, reverse transcription to cDNA, capture and enrichment with streptavidin beads, and PCR amplification.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-08-30T17:02:51Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-08-17T16:00:32Z
attributes.tag: External Id
attributes.value: SAMEA4837243
attributes.tag: INSDC center name
attributes.value: HARVARD UNIVERSITY
attributes.tag: INSDC first public
attributes.value: 2018-08-30T17:02:51Z
attributes.tag: INSDC last update
attributes.value: 2018-08-17T16:00:32Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7147:Sample 12
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: A375
attributes.tag: cell type
attributes.value: epithelial cell
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: malignant melanoma
attributes.tag: organism part
attributes.value: skin
attributes.tag: sample name
attributes.value: E-MTAB-7147:Sample 12
attributes.tag: scientific_name
attributes.value: Homo sapiens