organism: Mus musculus
description: Protocols: Enriched fractions of primary spermatocytes and round spermatids were obtained using 2 to 3 mice per experiment (i.e. 4 to 6 testes) by centrifugal elutriation. Around 1 million spermatocytes and round spermatids were used for ChIP-seq analyses. The chromatin was prepared by Diagenode using the iDeal ChIP-seq kit for histones (Diagenode Cat# C01010059) and then sheared using the Bioruptor Pico sonication device (Diagenode Cat# B01060001) combined with the Bioruptor Water cooler. ChIP was performed using the IP-Star Compact Automated System (Diagenode Cat# B03000002). The antibody H3K27cr was used at 1 µg per IP and each cell type was analyzed in two biological replicates. Libraries were prepared from input and immuno-purified DNA with 1ng as starting material using MicroPlex Library Preparation Kit v2 (Diagenode Cat# C05010013), then purified using Agencout® AMPPure® XP (Beckman Coulter) and quantified using Qubit™ dsDNA HS Assay Kit (Thermo Fisher Scientific, Q32854).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-02-04T04:03:16Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-09-13T09:42:59Z
attributes.tag: External Id
attributes.value: SAMEA5953228
attributes.tag: INSDC center name
attributes.value: CNRS
attributes.tag: INSDC first public
attributes.value: 2020-02-04T04:03:16Z
attributes.tag: INSDC last update
attributes.value: 2019-09-13T09:42:59Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8328:SC_Rep1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: spermatocyte
attributes.tag: common name
attributes.value: house mouse
attributes.tag: organism part
attributes.value: testis
attributes.tag: sample name
attributes.value: E-MTAB-8328:SC_Rep1
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: C57BL/6