organism: Homo sapiens
description: Protocols: The following cells were sorted from human children healthy donor tonsils: naive CD4+CD45RO- T cells (Naive) and CD4+CD45RO+CD25-CXCR5hiPD1hi follicular helper CD4+ T cells (TFH). For each of the TFH and Naive libraries, 50,000 live cells were harvested. The ATAC-seq libraries were generated following a previously published protocol (Buenrostro et al., Nature Methods, 2013). The nuclei were isolated from the 50,000 cells. The isolated nuclei were treated with Tn5 Transposes from Nextera DNA Sample Preparation Kit and the transposed DNA was purified using a Qiagen MinElute Kit. The transposed DNA libraries were amplified by PCR for 12 cycles and isolated by Ampure XP beads.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-08-31T17:03:36Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-23T15:03:15Z
attributes.tag: External Id
attributes.value: SAMEA104717430
attributes.tag: INSDC center name
attributes.value: The Children's Hospital of Philadelphia
attributes.tag: INSDC first public
attributes.value: 2018-08-31T17:03:36Z
attributes.tag: INSDC last update
attributes.value: 2018-03-23T15:03:15Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6617:Sample 4
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: CD4-positive helper T cell
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: juvenile stage
attributes.tag: disease
attributes.value: normal
attributes.tag: individual
attributes.value: TC58
attributes.tag: organism part
attributes.value: tonsil
attributes.tag: phenotype
attributes.value: CD4+CD45RO
attributes.tag: sample name
attributes.value: E-MTAB-6617:Sample 4
attributes.tag: scientific_name
attributes.value: Homo sapiens