organism: Homo sapiens
description: Protocols: RNA was isolated from 1-2 million cells using an RNeasy kit from Qiagen. GR18 cells where grown to approximately 90% confluence according to standard protocols in DMEM medium with 5% FBS. Cells were treated for 4 hours with 1uM Dexamethasone or 0.1% ethanol as vehicle control Prior to reverse transcription, poly adenylated RNA was isolated using oligo d(T) beads. Sequencing libraries were prepared using the TruSeq RNA library Prep Kit (Illumina)
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-09-01T17:02:13Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-04-30T14:27:14Z
attributes.tag: External Id
attributes.value: SAMEA4623832
attributes.tag: INSDC center name
attributes.value: Institut de Biologie de l'Ecole Normale Superieure
attributes.tag: INSDC first public
attributes.value: 2018-09-01T17:02:13Z
attributes.tag: INSDC last update
attributes.value: 2018-04-30T14:27:14Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6738:RNA-seq_Eth_rep3
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: U2OS
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: osteosarcoma
attributes.tag: genotype
attributes.value: expression of rat glucocorticoid receptor alpha
attributes.tag: organism part
attributes.value: bone tissue
attributes.tag: sample name
attributes.value: E-MTAB-6738:RNA-seq_Eth_rep3
attributes.tag: scientific_name
attributes.value: Homo sapiens