organism: Zaire ebolavirus
description: This stock was propagated from the p2 Ebola virus Makona-C05 Master stock (BioSample SAMN04488486, IRF0136_EBOV) in VERO C1008 (E6) cells, Kidney (African green monkey), Working Cell Bank, NR-596 obtained through BEI Resources (National Institute of Allergy and Infectious diseases [NIAID], National Institutes of Health [NIH], Manassas, VA). Virus stock was propagated using Minimum Essential Medium-alpha, GlutaMAX, no nucleosides (Gibco, ThermoFisher Scientific) supplemented with 2% US-origin, certified, heat-inactivated fetal bovine serum (HI-FBS, Gibco, ThermoFisher Scientific). Following harvest, HI-FBS was QS'd to 10% final concentration prior to cryopreservation.
attributes.tag: isolate
attributes.value: Ebola virus/H.sapiens-tc/GIN/2014/Makona-C05
attributes.tag: collected_by
attributes.value: Public Health Agency Canada (PHAC)
attributes.tag: collection_date
attributes.value: 2014-03-19
attributes.tag: geo_loc_name
attributes.value: Guinea: Gueckedou
attributes.tag: host
attributes.value: Homo sapiens
attributes.tag: host_disease
attributes.value: Ebola Virus Disease
attributes.tag: isolation_source
attributes.value: blood
attributes.tag: lat_lon
attributes.value: n/a
attributes.tag: host_disease_outcome
attributes.value: Patient survived
attributes.tag: host_sex
attributes.value: female
attributes.tag: passage_history
attributes.value: Vero E6 passage 3
attributes.tag: Passage Date
attributes.value: 20-Oct-2014 to 25-Oct-2014
attributes.tag: Laboratory Host
attributes.value: VERO C1008 (E6) cells
attributes.tag: Stock production
attributes.value: Integrated Research Facility (Honko, A.N.; Sword, J.M.; Jahrling, P.B.; Hensley, L.E.; Johnson, J.C.)
attributes.tag: Extraction method
attributes.value: TrizolLS
attributes.tag: BioSampleModel
attributes.value: Pathogen.cl