organism: Arabidopsis thaliana
description: Protocols: Surface sterilized Arabidopsis thaliana seeds were sown on 0.5x Murashige and Skoog (MS) media (Duchefa, Netherlands) solidified with 0.8 % Agar (Duchefa, Netherlands) at pH 5.8 (adjusted with MES). After two days of stratification at 4 °C plates were transferred to growth chambers (Rumed, Rubarth Apparate GmbH) with a photoperiod of 16 h 22 °C: 8 h 18 °C, light : dark at 120-150 μmol m-2s-1 light intensity. Total RNA was extracted from approximately 75-100 mg mg frozen plant material with the SV Total RNA Isolation System (Promega Corporation, Madison, WI, USA). Quality of RNA was measured using an Agilent 2100 Bioanalyzer (Agilent Technologies). rRNA was removed with the Ribo-Zero Plant rRNA removal kit for plant leafs (Illumina). RNA libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit (New England Biolabs).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-04-10T17:01:51Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2017-05-16T18:32:17Z
attributes.tag: External Id
attributes.value: SAMEA104015205
attributes.tag: INSDC center name
attributes.value: unspecified
attributes.tag: INSDC first public
attributes.value: 2018-04-10T17:01:51Z
attributes.tag: INSDC last update
attributes.value: 2017-05-16T18:32:17Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-5687:cipk326mock1_S13_R1_001
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: thale cress
attributes.tag: developmental stage
attributes.value: 12 day old seedlings
attributes.tag: ecotype
attributes.value: Col-0
attributes.tag: genotype
attributes.value: cipk3cipk26
attributes.tag: sample name
attributes.value: E-MTAB-5687:cipk326mock1_S13_R1_001
attributes.tag: scientific_name
attributes.value: Arabidopsis thaliana