organism: Homo sapiens
description: Protocols: RNA was extracted from treated cells using the RNeasy midi kit from Qiagen. GR18 cells were grown to approximately 90% confluence according to standard protocols in DMEM medium with 5% FBS 5 million U2OS-GR cells were transfected with 5 μg library-DNA by nucleofection using kit V (Lonza). The next day, cells were treated for 4 h with 1 µM dexamethasone or with 0.1% ethanol as vehicle control. The poly(A) fraction was isolated from total RNA using oligo d(T) beads. RNA was treated with turboDNase (Ambion) for 30 minutes at 37°C and cleaned up using the RNeasy MinElute kit (Qiagen). Reverse transcription and amplification of cDNA for subsequence Illumina sequencing were done as described (PMID 23328393).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-09-01T17:02:13Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-04-30T14:23:38Z
attributes.tag: External Id
attributes.value: SAMEA4623820
attributes.tag: INSDC center name
attributes.value: Institut de Biologie de l'Ecole Normale Superieure
attributes.tag: INSDC first public
attributes.value: 2018-09-01T17:02:13Z
attributes.tag: INSDC last update
attributes.value: 2018-04-30T14:23:38Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6737:Sample 8
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: U2OS
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: osteosarcoma
attributes.tag: genotype
attributes.value: expression of rat glucocorticoid receptor alpha
attributes.tag: organism part
attributes.value: bone tissue
attributes.tag: sample name
attributes.value: E-MTAB-6737:Sample 8
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: synthetic library
attributes.value: Sgk and Cgt GBS