organism: Mus musculus
description: Protocols: Skin draining lymph nodes (inguinal, axillary and brachial, unless otherwise noted) were collected and incubated in RPMI medium containing 2% FBS, 0.1mg/ml DNAse I (Invitrogen), 0.2mg/ml collagenase P (Roche) and 0.8 mg/ml Dispase (Roche). Sorted cells were loaded onto a 10x Chromium Chip using reagents from the Chromium Single Cell 3' Library Kit (10x Genomics) according to the manufacturer's protocol. Amplified cDNA was used for 3' RNA-seq library generation, RNA-seq libraries were prepared following themanufacturer's user guide (10x Genomics).
attributes.tag: ENA first public
attributes.value: 2021-04-10
attributes.tag: ENA last update
attributes.value: 2021-03-10
attributes.tag: External Id
attributes.value: SAMEA8228452
attributes.tag: INSDC center alias
attributes.value: Genentech
attributes.tag: INSDC center name
attributes.value: Genentech
attributes.tag: INSDC first public
attributes.value: 2021-04-10T00:31:40Z
attributes.tag: INSDC last update
attributes.value: 2021-03-10T20:02:24Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-10197:Replicate 1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: stromal cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: normal
attributes.tag: immunophenotype
attributes.value: CD45 negative
attributes.tag: individual
attributes.value: pool of mice 1 to 5
attributes.tag: organism part
attributes.value: mixed pool of axillary lymph node; brachial lymph node; inguinal lymph node
attributes.tag: sample name
attributes.value: E-MTAB-10197:Replicate 1
attributes.tag: strain
attributes.value: C57BL6/J