organism: Mus musculus
description: Protocols: 5x10^6 naive CD8 T cells from Cor93 TCR transgenic mice (BC10.3) were transferred into C57BL/6 wild-type or MUP-core recipients. Mice were splenectomized and treated with anti-CD62L Abs 48 hours and 4 hours prior to cell transfer, respectively. Wild-type mice were injected with 2.5*10^5 infectious units of non-replicating rLCMV-core 4 hours prior CD8 T cell transfer. A group of MUP-core mice received IL-2/anti-IL-2 complexes one day after CD8 T cell transfer. Livers were collected and analyzed at day 5 after CD8 T cell transfer. ReliaPrep RNA MiniPrep System (Promega) RNA-Seq libraries were prepared with the SMART-Seq2 protocol, with minor modification. Briefly, RNA (1-5 ng) was reverse transcribed using custom oligodT and template-switching LNA oligos (sequences), followed by PCR amplification and clean-up (Ampure XP beads, Beckman Coulter). The resulting cDNA (0,5- 1 ng) was tagmented at 55°C for 30 minutes and final RNA-Seq libraries generated using reagents from the Nextera XT DNA Library Prep Kit (Illumina).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2019-08-10T04:03:21Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-07-31T10:42:31Z
attributes.tag: External Id
attributes.value: SAMEA5819758
attributes.tag: INSDC center name
attributes.value: San Raffaele Telethon Institute for Gene Therapy.
attributes.tag: INSDC first public
attributes.value: 2019-08-10T04:03:21Z
attributes.tag: INSDC last update
attributes.value: 2019-07-31T10:42:31Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-74621564565901:MUP-core_day5_r2
attributes.tag: age
attributes.value: 8 to 10
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: CD8 T cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: Tg[MUP,HBVcore]Chi5O)
attributes.tag: growth condition
attributes.value: transplanted with naive CD8+ ve T cells from Cor93 TCR (BC10.3) transgenic mice
attributes.tag: individual
attributes.value: 2
attributes.tag: organism part
attributes.value: liver
attributes.tag: sample name
attributes.value: E-MTAB-74621564565901:MUP-core_day5_r2
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: C57BL/6