organism: Mus musculus
description: Protocols: Hearts were harvested in icecold PBS with 0.5 mM EDTA and dissociated as described (Van Handel et al. 2012), using collagenase (10 mg/ml; Worthington) and dispase (10 mg/mL, Invitrogen). Cells were stained for 30 minutes, 4',6-diamidino-2-phenylindole (DAPI, 1:1000) was used to label dead cells. Tie2Cre lineage cells, negative for blood markers CD41, CD45 and Ter119, were sorted on an InfluxTM cell sorter (BD Biosciences) and collected in RNA lysis buffer (Agilent) or TRIzol reagent (Ambion) for RNA extraction, or PBS/5% serum for ATAC-Seq (Buenrostro et al. 2013). Van Handel B, Montel-Hagen A, Sasidharan R, Nakano H, Ferrari R, Boogerd CJ, Schredelseker J, Wang Y, Hunter S, Org T, et al. 2012. Scl represses cardiomyogenesis in prospective hemogenic endothelium and endocardium. Cell 150: 590605. Buenrostro JD, Giresi PG, Zaba LC, Chang HY, Greenleaf WJ. 2013. Transposition of native chromatin for fast and sensitive epigenomic profiling of open chromatin, DNA-binding proteins and nucleosome position. Nat Methods 10: 12138. We performed ATAC-seq according to Buenrostro JD, Giresi PG, Zaba LC, Chang HY, Greenleaf WJ. 2013. Transposition of native chromatin for fast and sensitive epigenomic profiling of open chromatin, DNA-binding proteins and nucleosome position. Nat Methods 10: 12138.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2016-06-30T17:01:22Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-09T09:21:22Z
attributes.tag: External Id
attributes.value: SAMEA3627871
attributes.tag: INSDC center name
attributes.value: University of Chicago
attributes.tag: INSDC first public
attributes.value: 2016-06-30T17:01:22Z
attributes.tag: INSDC last update
attributes.value: 2018-03-09T09:21:22Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-3972:Sample 2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: endocardial
attributes.tag: common name
attributes.value: house mouse
attributes.tag: date
attributes.value: 2014-11-05
attributes.tag: developmental stage
attributes.value: embryonic day 12.5
attributes.tag: phenotype
attributes.value: wild-type
attributes.tag: replicate
attributes.value: 2
attributes.tag: sample name
attributes.value: E-MTAB-3972:Sample 2
attributes.tag: scientific_name
attributes.value: Mus musculus