organism: Mus musculus
description: Protocols: GV oocytes and zygotes were collected and mixed from three mice of each genotypes separately. Each group contained a total of 30 oocytes or zygotes. In the initial step, we spiked 2 × 106 mRNA-EGFP, transcribed in vitro, into each group. Each cell sample after sorting can be stored in 6μl Discover-sc™ WTA Kit V2(Vazyme, N711, Nanjing, China) sample buffer at -70°C. The volume of fresh cells was measured, and Discover-sc™ WTA Kit V2 was used for cell lysis and first strand cDNA synthesis. Then, the first stand cDNA was amplified by Discover-sc PCR Mix. The amplified double stranded cDNA was purified by AMPure XP beads. The PCR products were purified by AMPure XP beads and the final library was obtained.
attributes.tag: ENA first public
attributes.value: 2021-03-23
attributes.tag: ENA last update
attributes.value: 2021-03-11
attributes.tag: External Id
attributes.value: SAMEA8228527
attributes.tag: INSDC center alias
attributes.value: Nanjing Medical University
attributes.tag: INSDC center name
attributes.value: Nanjing Medical University
attributes.tag: INSDC first public
attributes.value: 2021-03-23T08:54:54Z
attributes.tag: INSDC last update
attributes.value: 2021-03-11T08:16:29Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-10199:WT_GV_1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: individual
attributes.value: pool of mice
attributes.tag: organism part
attributes.value: oocyte
attributes.tag: sample name
attributes.value: E-MTAB-10199:WT_GV_1
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: C57B6/L