organism: Arabidopsis thaliana
description: Protocols: The total tissues from 3-week-old WT and dja6 dja5 seedlings were collected and frozen quickly in liquid nitrogen before the extraction of total RNA. Arabidopsis thaliana Columbia-0 wild type and dja6 dja5 mutant seeds were pre-incubated in darkness for 48 h at 4℃ and sowed on the Murashige and Skoog (MS) medium containing 2% sucrose after surface-sterilization. Seedlings were grown on the MS medium for 3 weeks under a photoperiod of 12 h light/12 h dark cycle with a photon flux density of 120 μmol m-2 s-1 at 22℃. Total RNA was extracted using an RNeasy Plant Mini Kit (Cat No. 74904, Qiagen, Germany). The RNA sample was measured using Thermo NanoDrop 2000 (USA). The RNA sample was enriched using the Oligo (dT) magnetic beads, and subsequently fragmented in fragmentation buffer and revised into cDNA library. The samples were further purified using AMPure XP beads(Beckman Coulter, Brea CA, USA). Repair the ends of the double-stranded cDNA, tail the PCR products with adenosine and add the adapters. The sample were re-purified using AMPure XP beads. PCR amplify the cDNA.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2021-03-23T08:56:59Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-09-14T14:37:20Z
attributes.tag: External Id
attributes.value: SAMEA7306512
attributes.tag: INSDC center name
attributes.value: Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
attributes.tag: INSDC first public
attributes.value: 2021-03-23T08:56:59Z
attributes.tag: INSDC last update
attributes.value: 2020-09-14T14:37:20Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-9575:dja6dja5_2
attributes.tag: age
attributes.value: 3
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: thale cress
attributes.tag: developmental stage
attributes.value: LP.07 seven leaves visible stage
attributes.tag: genotype
attributes.value: dja6; dja5 double mutant
attributes.tag: organism part
attributes.value: whole organism
attributes.tag: sample name
attributes.value: E-MTAB-9575:dja6dja5_2
attributes.tag: scientific_name
attributes.value: Arabidopsis thaliana
attributes.tag: ecotype
attributes.value: Col-0