organism: Homo sapiens
description: Protocols: Cells were treated with LNA gapmer oligonucleotides at 25 nanomolar either against the long non-coding RNA RAIN or negative control antisense oligonucleotides. Cells collected 48h after reverse transfection. Long non-coding RAIN was described by Sancisi et al. in: RUNX2 expression in thyroid and breast cancer requires the cooperation of three non-redundant enhancers under the control of BRD4 and c-JUN. Nucleic Acids Res. 2017 doi: 10.1093/nar/gkx802. Genebank sequence IDs: MH084478 - MH084485 RNA was extracted using Qiagen RNAeasy collection kit Library were prepared using Truseq Stranded mRNA sample preparation kit (Illumina) starting from 1 microgram of total RNA
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2018-09-01T17:02:12Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-04-03T16:50:52Z
attributes.tag: External Id
attributes.value: SAMEA1049532
attributes.tag: INSDC center name
attributes.value: Laboratory of translational research. Azienda USL-IRCCS Reggio Emilia Italy
attributes.tag: INSDC first public
attributes.value: 2018-09-01T17:02:12Z
attributes.tag: INSDC last update
attributes.value: 2018-04-03T16:50:52Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-6622:long_2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: TPC1
attributes.tag: cell type
attributes.value: epithelial cell
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: papillary thyroid carcinoma
attributes.tag: organism part
attributes.value: thyroid gland
attributes.tag: rna interference
attributes.value: LNA gapmer against long non-coding RNA-RAIN
attributes.tag: sample name
attributes.value: E-MTAB-6622:long_2
attributes.tag: scientific_name
attributes.value: Homo sapiens