organism: Mus musculus
description: Protocols: Embryonic Tbx20-GFP mouse hearts 11.5dpf were dissected and cross-linked in1.8% of formaldehyde for 10 minutes at room temperature. Samples were quenched by incubation with 2.5 M glycine for 5 minutes. Cells were washed and resuspended in Lysis buffer (1% SDS, 10 mM EDTA, 50 mM Tris, pH 8.1). The cross-linked chromatin was sonicated using a Misonix 4000 (amplitude 90 and 30 seconds on/30 seconds off) to an average size of 200 bp. Precleared chromatin extract was incubated overnight at 4AC with goat anti-GFP and immunoprecipitated with protein AaSepharose beads. 10 ng of DNA was used to generate a standard Illumina sequencing library. Enrichment of selected target regions was confirmed by qRT-PCR. Illumina's standard protocol was used
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2016-06-30T17:01:22Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-09T08:56:58Z
attributes.tag: External Id
attributes.value: SAMEA3627869
attributes.tag: INSDC center name
attributes.value: University of Chicago, Dept of Human Genetics
attributes.tag: INSDC first public
attributes.value: 2016-06-30T17:01:22Z
attributes.tag: INSDC last update
attributes.value: 2018-03-09T08:56:58Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-3967:Sample 2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: embryonic day 11.5
attributes.tag: organism part
attributes.value: whole heart
attributes.tag: phenotype
attributes.value: wild-type
attributes.tag: sample name
attributes.value: E-MTAB-3967:Sample 2
attributes.tag: scientific_name
attributes.value: Mus musculus