organism: Saccharomyces cerevisiae
description: Protocols: Grown in SCD-His media at 30oC to exponential phase (OD600 0.5-0.7), pelleted by centrifugation and snap frozen into liquid nitrogen. Samples lysed in Lola lysis buffer using a 6870 Freezer Mill (Spex). Lysates cleared by centrifugating twice at 15000 g. Nucleic acids extracted using Trizol Reagent. rRNA was depleted from samples using RibominusTM Eukaryote Kit for RNA-Seq (Invitrogen). Depleted samples were precipitated with 2.5x volumes ethanol, 1/10th volume 3M sodium acetate and 1μl glycogen, washed twice with 70% ethanol and re-suspended in 10 μl DEPC water. Sequencing libraries were generated using the whole Transcriptome Library preparation protocol provided with the SOLiD® Total RNA-Seq Kit.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2016-03-31T17:36:25Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2018-03-08T23:56:54Z
attributes.tag: External Id
attributes.value: SAMEA3311347
attributes.tag: INSDC center name
attributes.value: The University of Manchester
attributes.tag: INSDC first public
attributes.value: 2016-03-31T17:36:25Z
attributes.tag: INSDC last update
attributes.value: 2018-03-08T23:56:54Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-3407:JCSEQ137
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: baker's yeast
attributes.tag: genotype
attributes.value: Mat a; his3 1; leu2 0; met15 0; ura3 0; YLL013c::kanMX4
attributes.tag: sample name
attributes.value: E-MTAB-3407:JCSEQ137
attributes.tag: scientific_name
attributes.value: Saccharomyces cerevisiae
attributes.tag: sex
attributes.value: Mat a
attributes.tag: specimen with known storage state
attributes.value: fresh specimen
attributes.tag: strain
attributes.value: BY4741