organism: endophyte metagenome
description: Pine wilt disease (PWD) is a devastating forest disease present worldwide. In this study we analyzed the effects of the invasion of Bursaphelenchus xylophilus, the major pathogen causing PWD, on the endophytic microbiome of adult P. pinaster trees. Wood samples from trees with different degrees of PWD disease were collected at Arganil (site M) and Oliveira do Hospital (site A), Portugal. Endophytic bacteria were characterized by DGGE fingerprinting and barcoding.
attributes.tag: isolation_source
attributes.value: Pinus Pinaster
attributes.tag: collection_date
attributes.value: 2010
attributes.tag: geo_loc_name
attributes.value: Portugal: close to Coimbra
attributes.tag: lat_lon
attributes.value: 40.28409 N 8.07307000 W
attributes.tag: ref_biomaterial
attributes.value: Proenca et al., 2010
attributes.tag: samp_collect_device
attributes.value: Samples were obtained by drilling a 5 mm diameter hole to a depth of 10 to 15 cm with a sterilized hand brace drill
attributes.tag: BioSampleModel
attributes.value: Metagenome or environmental