organism: Homo sapiens
description: Protocols: On day 7after purification, BMEC-like cells were detached from PA hydrogel surfaces with a 10 minuteAccutase incubation at 37°C. Cells from 3 gels of each stiffness condition were pooled to create one biological replicate. Cells were then rinsed with PBS and incubated in Trizol (Thermo Fisher 15596026) for 10 minutes at room temperature. RNA was extracted using a Direct-zol RNA miniprep kit (Zymo R2050) according to the manufacturer's instruction. This process was repeated two additional times from independent iPSC differentiations (biological n=3). Raw sequencing reads were obtained for the 9paired-end samples. FASTQ reads were mapped to human genome (hg19) by HISAT2 (version 2.1.0)
attributes.tag: ENA first public
attributes.value: 2021-04-08
attributes.tag: ENA last update
attributes.value: 2021-04-07
attributes.tag: External Id
attributes.value: SAMEA8535207
attributes.tag: INSDC center alias
attributes.value: Department of Biomedical Engineering, Vanderbilt University
attributes.tag: INSDC center name
attributes.value: Department of Biomedical Engineering, Vanderbilt University
attributes.tag: INSDC first public
attributes.value: 2021-04-08T00:31:49Z
attributes.tag: INSDC last update
attributes.value: 2021-04-07T14:29:52Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-10336:150kpa_0601
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: brain microvascular endothelial-like cell
attributes.tag: common name
attributes.value: human
attributes.tag: growth condition
attributes.value: 150 kPa matrix
attributes.tag: progenitor cell type
attributes.value: induced pluripotent stem cell
attributes.tag: sample name
attributes.value: E-MTAB-10336:150kpa_0601