organism: Gasterosteus aculeatus
description: Protocols: Adult sticklebacks were kept in artificial reproduction conditions in order to generate the in vitro fertilized embryos. Four couples were used to generate the embryos. 104 embryos were used for next generation sequencing analyses. 3 replicates of pool of 4 to 10 embryos per condition were used for NGS analyses. Each embryo was placed in individual glass tube and waterborne exposed to 3 mL of a radioactive solution of 0.1, 1 and 10 mGy/day using 32P and artificial water. These doses encompass the chronic low (0.1 mGy/day) and medium (1 mGy/day) doses encountered in the environment at Chernobyl 30 years after the accident, and the initial high dose to fish after the accident (10 mGy/day). These doses span the protection threshold of ecosystems set at 10 uGy/h (0.24 mGy/day). Control embryos were kept in clean artificial water. The embryos were exposed for 10 days and euthanized according to schedule 1 of the Home Office Licence (ASPA, 1986) using tricaine methanesulfonate (Sigma). Total RNAs were extracted using the High Pure RNA Tissue kit (Roche Diagnostics Ltd, West Sussex, U.K.) according to the supplier's instructions. RNA quality and integrity was evaluated using a bioanalyzer. RNA integrity numbers ranged from 7.4 to 9.5. mRNA-seq libraries were prepared for each pool using the Illumina TruSeq mRNA library kit following standard protocols.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2020-02-04T17:04:41Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2019-04-15T14:57:02Z
attributes.tag: External Id
attributes.value: SAMEA5567549
attributes.tag: INSDC center name
attributes.value: University of Portsmouth
attributes.tag: INSDC first public
attributes.value: 2020-02-04T17:04:41Z
attributes.tag: INSDC last update
attributes.value: 2019-04-15T14:57:02Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-7872:MediumExposure_Rep1
attributes.tag: age
attributes.value: 10 days post fertilisation
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: three-spined stickleback
attributes.tag: developmental stage
attributes.value: embryo
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: individual
attributes.value: mixed pool of 4 to 10 embryos
attributes.tag: lineage
attributes.value: Lineage 1
attributes.tag: organism part
attributes.value: whole organism
attributes.tag: sample name
attributes.value: E-MTAB-7872:MediumExposure_Rep1
attributes.tag: scientific_name
attributes.value: Gasterosteus aculeatus
attributes.tag: sex
attributes.value: mixed