organism: Homo sapiens
description: Protocols: PBMC from for donors were FcR blocked and stained with PE-conjugated HLA-A24:02 IAV PB1 498-505 tetramer. Cell were enriched using tetramer-associated-magnetic-enrichment and PE+CD3+CD8+ single cell sorted into a 96-well plate on BD Aria III. Single tetramer+ cells were sorted into 96-well plates containing 0.5 μl dNTP mix (10 mM), 0.5 μl oligo-dT pri- mer (5 μM), and 1 μl lysis buffer (prepared by adding 1 μl RNase inhibitor to 19 μl Triton X-100 solution, 0.2% v/v). Libraries were prepared using the Smart-seq2 protocol with the following modifications. cDNA synthesis and pre-amplification reaction volumes were halved to 5 and 12.5 μl, respectively. The final concentration of the ERCC RNA Spike-In Mix in the reverse-transcription step was 1:400 million.
attributes.tag: ENA first public
attributes.value: 2021-04-09
attributes.tag: ENA last update
attributes.value: 2021-02-16
attributes.tag: External Id
attributes.value: SAMEA8082419
attributes.tag: INSDC center alias
attributes.value: Department of Microbiology and Immunology, University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Parkville 3010, Victoria, Australia
attributes.tag: INSDC center name
attributes.value: Department of Microbiology and Immunology, University of Melbourne, at the Peter Doherty Institute for Infection and Immunity, Parkville 3010, Victoria, Australia
attributes.tag: INSDC first public
attributes.value: 2021-04-09T00:27:21Z
attributes.tag: INSDC last update
attributes.value: 2021-02-16T10:59:42Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-10109:Sample 90
attributes.tag: age
attributes.value: 38
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: CD8-positive, alpha-beta T cell
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: individual
attributes.value: Non-Lift 10
attributes.tag: organism part
attributes.value: blood
attributes.tag: phenotype
attributes.value: PB1 498-505+
attributes.tag: sample name
attributes.value: E-MTAB-10109:Sample 90
attributes.tag: sex
attributes.value: female