title: C16_Be2_IgD_neg
attributes.tag: source_name
attributes.value: B cells
attributes.tag: Sex
attributes.value: female
attributes.tag: sorting markers
attributes.value: CD19+ CD38mid CD27lo IgD- CD4-
attributes.tag: be type
attributes.value: Be2
attributes.tag: igd status
attributes.value: Negative
attributes.tag: culture conditions
attributes.value: Purified naive B cells were co-cultured for 6 days in the presence of IL-2 (50U/ml) and IL-21 (10ng/ml) with allogeneic in vitro generated Th2 effectors (0.6x10​6 cells/ml, ratio 5B:1T) in Iscove's DMEM supplemented with penicillin (200 microg/ml), streptomycin (200migrog/ml), gentamicin (40microg/ml), 10% FBS, transferrin (50microg/ml) and insuline (5microg/ml).
attributes.tag: donor
attributes.value: C16