title: siPSF-4
attributes.tag: sample_name
attributes.value: DRS016179
attributes.tag: sample comment
attributes.value: This sample is total RNA in the siPSF-4-depleted Hela TO cells.HeLa TO cells (Clontech) were grown in Dulbecco''s modified Eagle's medium (DMEM), supplemented with 10% fetal bovine serum (FBS) and antibiotics at 37C in a humidified incubator with 5% CO2.The siPSF-4 were transfected into cells using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer's instructions. Briefly, siPSF-4 duplexes (final concentration 10 nM) and cells were harvested 48 hr posttransfection.