title: SIP_bulk_T2_I
attributes.tag: collection_date
attributes.value: 2009-07
attributes.tag: depth
attributes.value: missing
attributes.tag: description
attributes.value: Microcosm of bulk soil spiked with diesel fuel oil incubated with 13C-naphtalene for 7 days. Soil was obtained from Great Northwest Inc. (Fairbanks, AK) and were a local blend of 60% silt and 40% peat.
attributes.tag: elev
attributes.value: missing
attributes.tag: env_biome
attributes.value: anthropogenic terrestrial biome
attributes.tag: env_feature
attributes.value: anthropogenic contamination
attributes.tag: env_material
attributes.value: oil contaminated soil
attributes.tag: geo_loc_name
attributes.value: USA: Fairbanks, AK
attributes.tag: lat_lon
attributes.value: missing
attributes.tag: organism
attributes.value: soil metagenome
attributes.tag: sample_title
attributes.value: SIP_bulk_T2_I
attributes.tag: seq_methods
attributes.value: The preparation of PCR products for pyrosequencing was based on a procedure modified from that described by Berry, et al. Regions V4-V6 of the 16S rRNA gene were amplified with primers 515-530 forward, 5-GTGCCAGCMGCNGCGG-3, and 1068-1052 reverse, 5-CTGRCGRCRRCCATGCA-3. Proportional amounts of each amplicon were mixed and then unidirectionally sequenced from the forward primer using the GS FLX Titanium chemistry followed by amplicons analysis of signal processing (Roche) at the Institute of Molecular Genetics, Czech Academy of Sciences, Prague, Czech Republic.
attributes.tag: BioSampleModel
attributes.value: MIMARKS.survey
attributes.tag: BioSampleModel
attributes.value: MIGS/MIMS/MIMARKS.soil