title: Extracellular polysaccharide-producing soil isolate Streptomyces sp. ME7.
attributes.tag: collection_date
attributes.value: 2015-02
attributes.tag: depth
attributes.value: 15-20 cm
attributes.tag: elev
attributes.value: 272 m
attributes.tag: env_biome
attributes.value: Agricultural Region
attributes.tag: env_feature
attributes.value: Farm
attributes.tag: env_material
attributes.value: Farm Soil
attributes.tag: geo_loc_name
attributes.value: USA: Columbia, Missouri, 10 m east of stable on side of low hill
attributes.tag: lat_lon
attributes.value: 38.869722 N 92.185278 W
attributes.tag: ref_biomaterial
attributes.value: In Preparation
attributes.tag: samp_collect_device
attributes.value: Sterilized Spatula
attributes.tag: samp_mat_process
attributes.value: Dropped soil sample into twirl pak and the sample was refrigerated within 30 minutes.
attributes.tag: strain
attributes.value: MOE7
attributes.tag: isol_growth_condt
attributes.value: Five grams of each soil sample were diluted in 50 ml ISP2 medium and the inoculated medium was placed in a 30 degree C shaker at 100 rpm for 60 min. Ten ml of three different dilutions (10-1, 10-2, 10-3) were prepared in sterile saline solutions 0.85% (wt/vol) NaCl. Then, 100 microliters of each dilution was cultured on ISP 2 agra medium containing 100 mg/L Nystatin as antifungal agent. Plates were incubated at 30 degrees C and monitored each 24 h for 14 days.The isolated Streptomyces sp. was then grown in a fermentation medium composed of the following in one liter (tryptone 5g, yeast extract 5g, glucose 10g, anhydrous K2HPO4 3g, KH2PO4 1g, NaCl 3g, MgSO4.7H2O 0.5g, anhydrous CaCO3 0.5g) pH 7.0 Manivasagan, et al., 2013
attributes.tag: num_replicons
attributes.value: None
attributes.tag: BioSampleModel
attributes.value: MIGS.ba
attributes.tag: BioSampleModel
attributes.value: MIGS/MIMS/MIMARKS.soil