title: siCTRL
attributes.tag: sample_name
attributes.value: DRS016177
attributes.tag: sample comment
attributes.value: This sample is total RNA in the siCTRL-depleted Hela TO cells.HeLa TO cells (Clontech) were grown in Dulbecco''s modified Eagle's medium (DMEM), supplemented with 10% fetal bovine serum (FBS) and antibiotics at 37C in a humidified incubator with 5% CO2.The siCTRL were transfected into cells using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer's instructions. Briefly, siCTRL duplexes (final concentration 10 nM) and cells were harvested 48 hr posttransfection.