title: siNEAT1_polyC_minus
attributes.tag: sample_name
attributes.value: DRS016175
attributes.tag: sample comment
attributes.value: This sample is total RNA in the siNEAT1-depleted Hela TO cells.HeLa TO cells (Clontech) were grown in Dulbecco''s modified Eagle's medium (DMEM), supplemented with10% fetal bovine serum (FBS) and antibiotics at 37C in a humidified incubator with 5% CO2. The siNEAT1 were transfected into cells using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer's instructions. Briefly, siNEAT1 duplexes (final concentration 10 nM) and cells were harvested 48 hr posttransfection.