attributes.tag: isolation_source
attributes.value: seawater
attributes.tag: collection_date
attributes.value: 2017-07-07
attributes.tag: geo_loc_name
attributes.value: Pacific Ocean: North Pacific Subtropical Gyre
attributes.tag: lat_lon
attributes.value: 24.72638 N 158.56058 W
attributes.tag: rel_to_oxygen
attributes.value: aerobe
attributes.tag: samp_collect_device
attributes.value: Niskin bottle collection from water column; followed by experimental incubation on in situ array; followed by filtration onto a 0.2 µm polyethersulfone filter
attributes.tag: samp_mat_process
attributes.value: Seawater was collected on trace-metal clean Niskin bottle casts and aliquoted into 2L polycarbonate bottles; +Fe treatments were amended with 2nM Fe as FeCl3 in a 10 mM HCl solution; and bottles were mounted on a surface-tethered in situ array and allowed to drift freely for 64 hours. After recovery, water was filtered onto a 0.2 µm polyethersulfone filter, preserved with RNA-later, and frozen at -80. Genomic DNA was extracted with the MasterPure Complete DNA Purification Kit (Lucigen); 16S rRNA gene amplicons were prepared with V4-V5 (515FY-926R) primers as recommended by Parada et al. 2016 and with multiplexing indexes as designed by the Earth Microbiome Project; and sequenced using PE250 v2 chemistry on an Illumina MiSeq. Note location is where water was originally collected, but collection date is when experiment was terminated.
attributes.tag: samp_size
attributes.value: 2.20 L
attributes.tag: Eddy_type
attributes.value: cyclone
attributes.tag: Depth
attributes.value: 100 m
attributes.tag: Treatment
attributes.value: plusFe
attributes.tag: Replicate
attributes.value: C
attributes.tag: BioSampleModel
attributes.value: Metagenome or environmental