title: UP3A Organic 2005
attributes.tag: isolation_source
attributes.value: organic horizon soil site UP3A
attributes.tag: collection_date
attributes.value: 2005-09-07
attributes.tag: geo_loc_name
attributes.value: USA: Alaska
attributes.tag: lat_lon
attributes.value: 64.76805555 N 148.27666666 W
attributes.tag: samp_collect_device
attributes.value: 2cm diameter soil corer
attributes.tag: samp_mat_process
attributes.value: Soil cores were collected, pooled and kept on liquid N until lyophilized. DNA was extracted using the MoBio PowerMax kit then normalized to 4ng/uL. Each soil sample was amplified using a barcoded primer with 7 replicates each. The primers included Illumina adaptors and golay barcodes. The base PCR primers were 5.8S_LT1 (AACTTTYRRCAAYGGATCWCT) and ITS4_mod_long (AGCCTCCGCTTATTGATATGCTTAART). Samples were pooled and sequenced in one 250bp PE MiSeq run.
attributes.tag: BioSampleModel
attributes.value: Metagenome or environmental