description: Wolbachia endosymbiont of Psylliodes chrysocephala genome assembly. The resulting individual used for HiFi and multi-individual used for Hi-C has a parasitic wasp present (10% genome size) so I had very good coverage of the wasp and got a good assembly of that. This went to the PhD PGI linked project. However the beetle coverage was then reduced to x12 so the assembly was not as continuous as it could have been. But despite that the assembly is reasonably good, chromosome level. Freebayes route used for error correction. I suggest to improve would be to repeat using non-infected individual HiFi. Included in the data set is 10X illumina library data of non-infected individual and male and female Illumina genome resequencing too. Manual curation and Hi-C to bring to chromosome level. Single primary sequence assembly, not haplotype assembled. Maker annotation pipeline used with both transcriptome transfer and de novo gene prediction as inputs to Evigene to pick best gene models. Curation of P450, ABC, UGT, IRAC gene models where RNA-seq evidence was present. Unknown sex individual used for 10X and then male 10X and female 10X samples. Single individual with parastoid wasp taken for PacBio HiFi. Single adult beetle from field sample collected at Rothamsted farm on 28 July 2017. GPS 51.8093, -0.3548
attributes.tag: ENA first public
attributes.value: 2022-06-14
attributes.tag: ENA last update
attributes.value: 2022-06-14
attributes.tag: External Id
attributes.value: SAMEA10422655
attributes.tag: INSDC center alias
attributes.value: ROTHAMSTED RESEARCH
attributes.tag: INSDC center name
attributes.value: ROTHAMSTED RESEARCH
attributes.tag: INSDC first public
attributes.value: 2022-06-14T00:18:14Z
attributes.tag: INSDC last update
attributes.value: 2022-06-14T00:18:14Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: Wolbachia_endosymbiont_of_Psylliodes chrysocephala_genome
attributes.tag: collection date
attributes.value: 2017
attributes.tag: common name
attributes.value: Wolbachia
attributes.tag: geographic location (country and/or sea)
attributes.value: United Kingdom
attributes.tag: sample name
attributes.value: Wolbachia_endosymbiont_of_Psylliodes chrysocephala_genome