description: The Metarhizium acridum strain ARSEF 324 was isolated in 1979 from a spur-throated locust (Austracris guttulosa) in Australia and obtained from the ARSEF collection (ARS Collection of Entomopathogenic Fungal Cultures, Ithaca, New York).
attributes.tag: strain
attributes.value: ARSEF 324
attributes.tag: collection_date
attributes.value: 1979-02
attributes.tag: env_broad_scale
attributes.value: terresterial ecosystem [ENVO_01001790]
attributes.tag: env_local_scale
attributes.value: vegetated area [ENVO_01001305]
attributes.tag: env_medium
attributes.value: organic material [ENVO_01000155]
attributes.tag: geo_loc_name
attributes.value: Australia: Queensland
attributes.tag: isol_growth_condt
attributes.value: Conidia from the single spore culture were grown in liquid sabouraud dextrose broth with yeast extract (40 g/L dextrose, 10 g/L peptone, 10 g/L yeast extract, pH = 6.5) while being stirred at 170 rpm on a shaking table for 24 hours before DNA extraction. Liquid culture filtrate was ground with liquid nitrogen in a mortar and ca. 500 mg finely ground powder transferred to 50 mL Falcon tube. DNA was extracted using a CTAB method by adding 17.5 mL CTAB buffer with 0.1% 2-Mercaptoethanol and 125 ul Proteinase K and incubated for one hour at 60 C. After centrifugation for 20 min. at 5.000g at 4 C, the supernatant was removed and washed with 1 volume of Phenol/chloroform/isoamyl alcohol (25:24:1) followed by two rounds of 1 volume chloroform/isoamyl alcohol (24:1) and centrifuged for 10 min at 11.000 rpm at each step. To remove RNA, 150 ul RNAse was added to the aqueous phase and incubated for 120 min. at 37 C. To precipitate DNA, 0.6 vol. 2-propanol was added and incubated at -20 C overnight, before centrifugation at 11.000 rpm at 4 C for 30 min. The resulting pellet was washed twice with 2 mL 70% Ethanol and centrifuged for 10 min at 11.000 rpm at 4 C before being suspended in 500 ul TE-buffer.
attributes.tag: lat_lon
attributes.value: 27.4746 S 152.9907 E
attributes.tag: biotic_relationship
attributes.value: symbiont
attributes.tag: estimated_size
attributes.value: 40 Mb
attributes.tag: host
attributes.value: Austracris guttulosa
attributes.tag: host_taxid
attributes.value: 909763
attributes.tag: isolation_source
attributes.value: M. acridum strain ARSEF 324 was isolated in 1979 from a spur-throated locust (Austracris guttulosa) in Australia and obtained from the ARSEF collection (ARS Collection of Entomopathogenic Fungal Cultures, Ithaca, New York).
attributes.tag: pathogenicity
attributes.value: grasshopper
attributes.tag: ploidy
attributes.value: diploid
attributes.tag: BioSampleModel
attributes.value: MIGS.eu