description: Protocols: At predefined time-points post-infection, animals were submitted to behavioral tests or euthanized, when samples of nasal turbinates, trachea, lungs and the brain (separated in olfactory bulbs, cerebellum, cortex and brainstem) were collected, immediately frozen at -80°C or formalin-fixed after transcardial perfusion with a physiological solution containing 5.103 U/mL heparin (choay, Sanofi) followed by 4% neutral buffered formaldehyde. Frozen tissues were homogenized with Trizol (15596026, Invitrogen) in Lysing Matrix D 2 mL tubes (116913100, MP Biomedicals) using the FastPrep-24™ system (MP Biomedicals) at the speed of 6.5 m/s during 1 min. Total RNA was extracted using the Direct-zol RNA MicroPrep Kit (R2062, Zymo Research). RNA preparation was used to construct strand specific single end cDNA libraries according to manufacturers' instructions (Truseq Stranded mRNA sample prep kit, Illumina)
attributes.tag: ENA first public
attributes.value: 2021-06-19
attributes.tag: ENA last update
attributes.value: 2021-06-19
attributes.tag: External Id
attributes.value: SAMEA8717521
attributes.tag: INSDC center alias
attributes.value: 1. Plate-forme Technologique Biomics - Centre de Ressources et Recherches Technologiques (C2RT), Institut Pasteur, Paris, France 2. Hub de Bioinformatique et Biostatistique - Departement Biologie Computationnelle, Institut Pasteur, 75015 Paris, France
attributes.tag: INSDC center name
attributes.value: 1. Plate-forme Technologique Biomics - Centre de Ressources et Recherches Technologiques (C2RT), Institut Pasteur, Paris, France 2. Hub de Bioinformatique et Biostatistique - Departement Biologie Computationnelle, Institut Pasteur, 75015 Paris, France
attributes.tag: INSDC first public
attributes.value: 2021-06-19T00:18:27Z
attributes.tag: INSDC last update
attributes.value: 2021-06-19T00:18:27Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-10442:136
attributes.tag: age
attributes.value: 5 to 6
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: golden hamster
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: COVID-19
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: individual
attributes.value: 136
attributes.tag: infect
attributes.value: SARS-CoV-2
attributes.tag: organism part
attributes.value: olfactory bulb
attributes.tag: sample name
attributes.value: E-MTAB-10442:136
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: RjHan:AURA