description: Protocols: All experimental mice were sacrificed and the livers were recovered. Approximately 5 mg of liver tissue from high-fat (HFD) diet mice, both eIF6 wt and eIF6 heterozygous, were homogenized and RNA was purified Total RNA from liver tissues was purified using mirVanaTM isolation kit (ThermoFisher) according to the manufacturer protocols. RNA quality was assessed using the Agilent RNA Pico 6000 Kit and BioAnalyzer (Agilent Technologies) Random primed cDNA libraries were constructed from 100 ng of total RNA.
attributes.tag: ENA first public
attributes.value: 2021-06-18
attributes.tag: ENA last update
attributes.value: 2021-06-18
attributes.tag: External Id
attributes.value: SAMEA6808336
attributes.tag: INSDC center alias
attributes.value: Istituto Nazionale Genetica Molecolare
attributes.tag: INSDC center name
attributes.value: Istituto Nazionale Genetica Molecolare
attributes.tag: INSDC first public
attributes.value: 2021-06-18T16:16:29Z
attributes.tag: INSDC last update
attributes.value: 2021-06-18T16:16:29Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-9009:HFD_WT_149
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: diet
attributes.value: high fat diet
attributes.tag: disease
attributes.value: non-alcoholic fatty liver disease
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: liver
attributes.tag: sample name
attributes.value: E-MTAB-9009:HFD_WT_149