description: Cortex tissue from fruit harvested from internal canopy of trees, IAD Class C (1.80-1.89 IAD). Pooled tissue was ground to a fine powder with a SPEX 6875 Freezer/Mill in the presence of liquid nitrogen. RNA was extracted using a CTAB/Chloroform protocol modified specifically for tree fruit tissue.
attributes.tag: tissue
attributes.value: Cortex
attributes.tag: treatment
attributes.value: after 3 Months CA Storage (− 0.5 °C, 2 kPa O2 and 0.8 kPa CO2)
attributes.tag: temp
attributes.value: 0.5 degrees Celcius
attributes.tag: cultivar
attributes.value: d'Anjou'
attributes.tag: age
attributes.value: not applicable
attributes.tag: dev_stage
attributes.value: horticulturally mature fruit
attributes.tag: geo_loc_name
attributes.value: USA: a commercial orchard approximately 3 miles west of Cashmere, Washington
attributes.tag: biomaterial_provider
attributes.value: Washington State University Tree Fruit Research and Extension Center, 1100 N Western Ave, Wenatchee, WA 98801
attributes.tag: collected_by
attributes.value: Washington State University Tree Fruit Research and Extension Center
attributes.tag: collection_date
attributes.value: 2014-09-14
attributes.tag: isolation_source
attributes.value: A commercial orchard comprised of mature open vase trained 'd'Anjou' trees. 'd'Anjou' scion on 'Bartlett' seedling rootstock, 6m x 6m planting distance, 278 trees per ha with rows in an east to west oreientation planted in the 1970s was used.
attributes.tag: lat_lon
attributes.value: 47.5175 N 120.511447 W
attributes.tag: sample_type
attributes.value: Biological Replicates are pooled tissue samples from 3 individual pear fruits
attributes.tag: growth_protocol
attributes.value: commercial fruit production
attributes.tag: library_strategy
attributes.value: RNA-seq
attributes.tag: Library_preparation
attributes.value: Illumina TruSeq
attributes.tag: library_source
attributes.value: TRANSCRIPTOMIC
attributes.tag: library_selection
attributes.value: polyA
attributes.tag: library_layout
attributes.value: Fragment
attributes.tag: platform
attributes.value: Illumina
attributes.tag: instrument_model
attributes.value: Illumina HiSeq 2500 in Rapid Mode
attributes.tag: filetype
attributes.value: fastq
attributes.tag: filename1
attributes.value: DAP-1-14_091B_techrep1.fastq.gz
attributes.tag: filename2
attributes.value: DAP-1-14_091B_techrep2.fastq.gz
attributes.tag: filename3
attributes.value: DAP-1-14_091B_techrep3.fastq.gz
attributes.tag: filename4
attributes.value: DAP-1-14_091B_techrep4.fastq.gz
attributes.tag: filename5
attributes.value: DAP-1-14_091B_techrep5.fastq.gz
attributes.tag: BioSampleModel
attributes.value: Plant