description: Protocols: All mice used were C57BL/6J (The Jackson Laboratory, Stock # 00664), 8-9 weeks old and group-housed. The whole olfactory mucosa (WOM) of 9 mice was carefully dissected, and all the surrounding tissue (including glands and bone) removed. The WOMs were then embedded in OCT (Tissue Tek), immediately frozen in dry-ice and kept at -80ºC. Each WOM was then cryosectioned along each of the 3 cartesian axes: dorsal-ventral (D-V, N=3), anterior posterior (A-P, N=3), or lateral-medial-lateral (L-M-L, N=3). Every second cryosections (35 µM) was collected into 1.5 mL eppendorf tubes containing 350 µL RLT Plus Buffer (Qiagen) supplemented with 1% 2-mercaptoethanol, immediately frozen in dry-ice and kept at -80C until extraction. RNA was extracted using the RNeasy Plus Micro Kit (Qiagen), together with genomic DNA eliminator column and a 30 minute incubation with DNAse I (Qiagen). Reverse transcription and cDNA pre-amplification were performed using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Clontech/Takara). cDNA was harvested and quantified with the Bioanalyzer DNA High-Sensitivity kit (Agilent Technologies). Libraries were prepared using the Nextera XT DNA Sample Preparation Kit and the Nextera Index Kit (Illumina).
attributes.tag: ENA first public
attributes.value: 2022-02-17
attributes.tag: ENA last update
attributes.value: 2022-02-17
attributes.tag: External Id
attributes.value: SAMEA8386441
attributes.tag: INSDC center alias
attributes.value: Institute of Epigenetics and Stem Cells, Helmholtz Zentrum Munchen
attributes.tag: INSDC center name
attributes.value: Institute of Epigenetics and Stem Cells, Helmholtz Zentrum Munchen
attributes.tag: INSDC first public
attributes.value: 2022-02-17T20:44:00Z
attributes.tag: INSDC last update
attributes.value: 2022-02-17T20:44:00Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-10211:Sample 409
attributes.tag: age
attributes.value: 8 to 9
attributes.tag: axis
attributes.value: lateral-medial-lateral
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: fraction
attributes.value: WOM6-LML-44
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: individual
attributes.value: 6
attributes.tag: organism part
attributes.value: olfactory epithelium
attributes.tag: sample name
attributes.value: E-MTAB-10211:Sample 409
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: C57BL/6J