description: Saplings of F. septica naturally grown in a forest at the University of the Ryukyus (26.25 N 127.77 E) were transplanted into 30-L pots (one sapling per pot). The saplings were maintained under ambient conditions and irrigated 2-3 times per week before use. Only healthy, mature leaves of F. septica were selected for analysis. Variability of isoprene emission and photosynthesis of F. septica in response to temperature was examined using a leaf chamber, a real time isoprene analyser, and a portable photosynthesis analyser in a phytotron, as described previously (Oku et al., Chemosphere 95, 268-273, 2014). Light (300 micro mol m-2 s-1 of PPFD) and dark cycle was maintained at 12-h:12-h (L:D). The temperature regime to which the saplings were exposed was 30-24-18-12-30-30 deg C for over six days (i.e. temperature was lowered from 30 deg C to 12 deg C in 6 deg C daily increments over the first four days and then elevated again to 30 deg C in the latter two days). Hereafter, the first four days and latter two days are referred to as Day -4 to -1 and Day 1 to 2, respectively. Temperatures were changed when switching from the dark to light phase. Gene expression was analysed using a 5500xl SOLiD sequencer (Life Technologies, CA, USA).Samples were collected during the day at time points of 0.5 h (on Days 1 and 2) and 6 h (on Day -3 to 2), for a total of seven time points. A leaf per sapling was harvested at each sample time point.
attributes.tag: sample_name
attributes.value: Fs_IS740
attributes.tag: tissue_type
attributes.value: leaf