description: Protocols: Bone marrow (BM) was isolated from pooled femora, tibiae, ilia and vertebrae of C57BL6 mice by gentle crushing in PBS using a mortar and pistil. MPP5 progenitors were identified by immunophenotype (Lineage- Sca1+ Kit+ CD150- CD48- CD34+ CD135-), purified by FACS and subsequently subjected to 3'-seq. Total RNA isolation was performed using ARCTURUS PicoPure RNA isolation kit (Life Technologies, Invitrogen) according to the manufacturer's instructions. Total RNA was used for quality controls and for normalization of the starting material. Sequencing libraries were generated with 3 ng of total RNA using the QuantSeq 3' mRNA-Seq Library Prep Kit REV (Lexogen) according to the manufacturer's indications except for adaptations (version 015UG009V0221: Do not skip step 2; 15 minute incubation time step 4; Cycle Number was adjusted to 19 using the PCR Add-on Kit for Illumina (Lexogen))
attributes.tag: ENA first public
attributes.value: 2021-06-18
attributes.tag: ENA last update
attributes.value: 2021-06-18
attributes.tag: External Id
attributes.value: SAMEA6863993
attributes.tag: INSDC center alias
attributes.value: University of Heidelberg
attributes.tag: INSDC center name
attributes.value: University of Heidelberg
attributes.tag: INSDC first public
attributes.value: 2021-06-18T16:16:31Z
attributes.tag: INSDC last update
attributes.value: 2021-06-18T16:16:31Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-9135:MPP5a
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: hematopoietic multipotent progenitor cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: immunophenotype
attributes.value: Lineage- Sca1+ Kit+ CD150- CD48- CD34+ CD135-
attributes.tag: organism part
attributes.value: bone marrow
attributes.tag: replicate
attributes.value: 1
attributes.tag: sample name
attributes.value: E-MTAB-9135:MPP5a
attributes.tag: strain
attributes.value: C57BL/6J