description: Protocols: Retinas were isolated from E18.5, C57BL6 mouse embryos and digested with 0.05% trypsin (Gibco) for 15 minutes at 37 C. After 1 day of culture, cells were starved for glucose in Neobasal medium supplemented with B27 supplement without insulin, 0.06 g/L glutamine, 1% penicillin-streptomycin (Gibco) and 1 mM HEPES for 6 h. The cells were harvested for RNA isolation after additional 6 days of culturing. Normal cultured cells were used as control. RNA extracts were isolated by using TruSeq Stranded Total RNA with RiboZero. Reverse transcription was done by using RevertAid first strand cDNA synthesis kit (ThermoFisher)
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-06-26
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-06-26
attributes.tag: External Id
attributes.value: SAMEA9362127
attributes.tag: INSDC center alias
attributes.value: UNIVERSITY OF BERGEN
attributes.tag: INSDC center name
attributes.value: UNIVERSITY OF BERGEN
attributes.tag: INSDC first public
attributes.value: 2022-06-26T00:13:59Z
attributes.tag: INSDC last update
attributes.value: 2022-06-26T00:13:59Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-10705:Sample 10
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: cultured retinal cells
attributes.tag: cell_type
attributes.value: retinal cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: E18.5
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: growth condition
attributes.value: glucose starvation
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: retina
attributes.tag: sample name
attributes.value: E-MTAB-10705:Sample 10
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: C57BL6