attributes.tag: isolate
attributes.value: the intestinal tissues of Eimeria necatrix infection chickens
attributes.tag: breed
attributes.value: yellow-feathered chickens
attributes.tag: host
attributes.value: chicken
attributes.tag: isolation_source
attributes.value: sixty fifteen-day-old yellow-feathered chickens were infected with 1.0 104 sporulated E. necatrix oocysts. Infected intestinal tissues were removed after 136 h, cut longitudinally and rinsed three times with ice-cold PBS at pH 7.4. The mucosa was scraped using two glass slides and put into ten volumes of a solution containing 120 mM NaCl, 10 mM CaCl2, 3 mM K2HPO4, 20 mM Tris-HCl, 0.1% bovine serum albumin and 0.1% hyaluronidase and incubated for 1 h at 37 C in a thermostat water bath. Large intestinal debris was removed by filtering through gauze, and small debris was removed by sequential filtration through 17-m and 10-m polymon mesh (Sefar Filtration Solution Co. Ltd., Suzhou, China). The mixture was then centrifuged at 1400g for 10 min and the pellet was washed three times in ice-cold PBS. To remove red blood cells, the pellet was resuspended in lysis buffer (Solarbio, Beijing, China) and placed for 10 min at 4 C. After three washes with ice-cold PBS, the resulting MZ-2 were purified by density-gradient centrifugation using the method described by Mo et al.
attributes.tag: collection_date
attributes.value: 2020-10-26
attributes.tag: geo_loc_name
attributes.value: China: Yangzhou
attributes.tag: tissue
attributes.value: intestinal tissues
attributes.tag: BioSampleModel
attributes.value: Invertebrate