title: RNA-seq of Hs766T-L3
attributes.tag: isolate
attributes.value: The Hs766T-L3 was a subline of Hs766T with stable liver metastatic ability, which was isolated from the liver of BALB/c nude mice. For generation of Hs766T liver metastatic cancer cells, we established a pancreatic cancer spontaneous liver metastasis model. Briefly, Hs766T cells stably expressed luciferase were injected into the pancreases of BALB/c nude mice. After 14 weeks, cancer cells that spontaneously metastasized to the liver were separated, expanded and reinoculated into the pancreases of next-generation recipients. After three generations of screening, the third generation of Hs766T cells (Hs766T-L3) developed liver metastasis in 9/10 mice. Hs766T-L3 cells were collected and expanded.
attributes.tag: age
attributes.value: 22 weeks
attributes.tag: biomaterial_provider
attributes.value: Hen Lin, laboratory in institute of Hepatopancreatobiliary Surgery, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, China.
attributes.tag: sex
attributes.value: not determined
attributes.tag: tissue
attributes.value: from a pancreatic carcinoma metastatic to a liver
attributes.tag: BioSampleModel
attributes.value: Human