title: SARS-CoV-2: wastewater surveillance
attributes.tag: collection_date
attributes.value: 2022-03-22
attributes.tag: geo_loc_name
attributes.value: USA: Virginia
attributes.tag: isolation_source
attributes.value: wastewater
attributes.tag: collection_site_id
attributes.value: 51069
attributes.tag: ww_population
attributes.value: 16563
attributes.tag: ww_sample_duration
attributes.value: 24
attributes.tag: ww_sample_matrix
attributes.value: post grit removal
attributes.tag: ww_sample_type
attributes.value: composite
attributes.tag: collection_volume
attributes.value: 100mL
attributes.tag: ww_surv_target_1
attributes.value: SARS-CoV-2
attributes.tag: ww_surv_target_1_known_present
attributes.value: no
attributes.tag: collected_by
attributes.value: Virginia Division of Consolidated Laboratory Services
attributes.tag: purpose_of_ww_sampling
attributes.value: public health surveillance community-level
attributes.tag: ww_flow
attributes.value: 3000000
attributes.tag: ww_ph
attributes.value: 7.86
attributes.tag: ww_population_source
attributes.value: population of jurisdiction encompassing the wastewater service area
attributes.tag: ww_sample_site
attributes.value: wastewater treatment plant
attributes.tag: ww_temperature
attributes.value: 16
attributes.tag: purpose_of_ww_sequencing
attributes.value: diversity of mutations across entire genome
attributes.tag: sequenced_by
attributes.value: Virginia Division of Consolidated Laboratory Services
attributes.tag: ww_surv_target_1_gene
attributes.value: N gene (N2)
attributes.tag: ww_surv_target_1_protocol
attributes.value: ddPCR detection of SARS-CoV-2 using the BioRad PREvalence Kit on the BioRad QXOne
attributes.tag: ww_surv_target_2
attributes.value: SARS-CoV-2
attributes.tag: ww_surv_target_2_conc
attributes.value: 1862
attributes.tag: ww_surv_target_2_conc_unit
attributes.value: copies/L
attributes.tag: ww_surv_target_2_gene
attributes.value: E gene (orf4)
attributes.tag: ww_surv_target_2_known_present
attributes.value: no
attributes.tag: concentration_method
attributes.value: innovaprep ultrafiltration
attributes.tag: extraction_control
attributes.value: SARS-CoV-2 Standard spiked with MHV
attributes.tag: extraction_method
attributes.value: zymo quick-rna viral kit
attributes.tag: project_name
attributes.value: GenomeTrakr; LFFM ww project
attributes.tag: ww_surv_target_1_conc
attributes.value: 621
attributes.tag: ww_surv_target_1_conc_unit
attributes.value: copies/L
attributes.tag: ww_surv_jurisdiction
attributes.value: VA
attributes.tag: ww_processing_protocol
attributes.value: https://www.bio-rad.com/sites/default/files/webroot/web/pdf/lsr/literature/10000142158.pdf
attributes.tag: specimen_processing
attributes.value: Not Applicable
attributes.tag: collection_time
attributes.value: 06:30 - 06:30
attributes.tag: specimen_processing_details
attributes.value: Concentrating pipette
attributes.tag: BioSampleModel
attributes.value: SARS-CoV-2: wastewater surveillance