title: 37F.DNA.39.B
attributes.tag: ENA first public
attributes.value: 2021-04-17
attributes.tag: ENA last update
attributes.value: 2021-04-04
attributes.tag: External Id
attributes.value: SAMEA8512381
attributes.tag: INSDC center alias
attributes.value: UNIVERSITY OF CALIFORNIA - SAN DIEGO
attributes.tag: INSDC center name
attributes.value: UNIVERSITY OF CALIFORNIA - SAN DIEGO
attributes.tag: INSDC first public
attributes.value: 2021-04-17T00:26:21Z
attributes.tag: INSDC last update
attributes.value: 2021-04-04T04:05:12Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: 37F.DNA.39.B
attributes.tag: amount or size of sample collected
attributes.value: 2
attributes.tag: collection date
attributes.value: 2015-02-24
attributes.tag: common name
attributes.value: marine_sediment_metagenome
attributes.tag: environment (biome)
attributes.value: marine_abyssal_zone_biome
attributes.tag: environment (feature)
attributes.value: marine_oligotrophic_desert
attributes.tag: environment (material)
attributes.value: mud
attributes.tag: geographic location (country and/or sea)
attributes.value: Pacific Ocean
attributes.tag: geographic location (depth)
attributes.value: 4185
attributes.tag: geographic location (elevation)
attributes.value: 0
attributes.tag: geographic location (latitude)
attributes.value: 12.1178547
attributes.tag: geographic location (longitude)
attributes.value: -117.3434123
attributes.tag: geographic location (region and locality)
attributes.value: CCFZ:Abyssline2
attributes.tag: investigation type
attributes.value: mimarks-survey
attributes.tag: library construction method
attributes.value: TruSeq_Nano_PCR_free_Library_Preparation_Kit_single-end
attributes.tag: library reads sequenced
attributes.value: 1757175
attributes.tag: library screening strategy
attributes.value: normalized
attributes.tag: library size
attributes.value: 47
attributes.tag: library vector
attributes.value: TruSeq
attributes.tag: multiplex identifiers
attributes.value: F1-G:_{CGATGACAAAGGGCACCACAAGAACGC}_15-K:_{CATCGTACCCACCTATCACACAATCATG}
attributes.tag: nucleic acid amplification
attributes.value: https://github.com/FranckLejzerowicz/protocols
attributes.tag: nucleic acid extraction
attributes.value: https://www.qiagen.com/us/cmp/ecom/powersoilkits?COECM_MOBIO_Branded_1707_SEA_PowerSoil__&kwid=%2Bpower%20%2Bsoil%20%2Brna
attributes.tag: pcr conditions
attributes.value: initial_denaturation:95_5;_denaturation:_94_0.5;_annealing:52_0.2;_elongation:_72_1;_40_cycles;_final_elongation:72_2
attributes.tag: pcr primers
attributes.value: FWD:_{AAGGGCACCACAAGAACGC}_REV:_{CCACCTATCACACAATCATG}
attributes.tag: project name
attributes.value: DSMP
attributes.tag: sample collection device or method
attributes.value: multiple_corer
attributes.tag: sample material processing
attributes.value: lifeguard_preservation_solution
attributes.tag: sample name
attributes.value: 37F.DNA.39.B
attributes.tag: sample volume or weight for DNA extraction
attributes.value: 2
attributes.tag: sediment environmental package
attributes.value: sediment
attributes.tag: sequencing method
attributes.value: Illumina
attributes.tag: source material identifiers
attributes.value: DSMP_39
attributes.tag: target gene
attributes.value: 18S_rRNA
attributes.tag: target subfragment
attributes.value: 37F