title: RNA-Seq of Solanum lycopersicum: anthocyanin reduced (are) pollen tube at 28C
attributes.tag: cultivar
attributes.value: anthocyanin reduced (are)
attributes.tag: dev_stage
attributes.value: flowering
attributes.tag: geo_loc_name
attributes.value: USA: Greensboro, North Carolina
attributes.tag: tissue
attributes.value: pollen tube
attributes.tag: library_id
attributes.value: ARE.Control.1
attributes.tag: title
attributes.value: RNA-Seq of Solanum lycopersicum: anthocyanin reduced (are) pollen tube at 28C
attributes.tag: library_strategy_(click_for_details)
attributes.value: RNA-Seq
attributes.tag: library_source_(click_for_details)
attributes.value: TRANSCRIPTOMIC
attributes.tag: library_selection_(click_for_details)
attributes.value: PolyA
attributes.tag: library_layout
attributes.value: paired
attributes.tag: platform_(click_for_details)
attributes.value: ILLUMINA
attributes.tag: instrument_model
attributes.value: Illumina HiSeq 4000
attributes.tag: design_description
attributes.value: Anthers of flowers from each genotype were harvested into a 15mL falcon tube and resuspended in 5000uL of pollen germination media (PGM). 2000uL was removed and divided into two 1.5mL Eppendorf tubes per genotype and incubated in a 28C water bath for 30 minutes. One of the tubes was moved to a 34C water bath and both tubes were incubated for an additional 90 minutes. The samples were centrifuged, PGM removed, flash frozen in liquid nitrogen, and stored at -80 until extraction with the Qiagen RNeasy Plant Mini Kit.
attributes.tag: filetype
attributes.value: fastq
attributes.tag: filename
attributes.value: are-120min-28C-R1_R1_001.fastq.gz
attributes.tag: filename2
attributes.value: are-120min-28C-R1_R2_001.fastq.gz
attributes.tag: BioSampleModel
attributes.value: Plant