description: Protocols: Sections of formalin-fixed paraffin-embedded (FFPE) tissue were freshly cut for nucleic acid extraction. RNA was extracted from FFPE tissue sections using the AllPrep DNA/RNA FFPE Kit (Qiagen, Netherlands) according to the manufacturer's protocol. RNA was eluted in 20-30 µl RNase-free water. DNA and RNA concentrations were quantified using Qubit fluorometry (Invitrogen, Carlsbad, CA). Sequencing libraries were prepared according to manufacturer's instructions for either the TruSeq RNA Sample Prep Kit v2 or the TruSeq RNA Access Library Prep Kit (Illumina, San Diego, CA). Library concentration and size distribution were initially determined using an Agilent Bioanalyzer DNA 1000 chip (Santa Clara, CA), and Qubit fluorometry (Invitrogen, Carlsbad, CA) was performed to confirm concentration.
attributes.tag: ENA first public
attributes.value: 2021-04-14
attributes.tag: ENA last update
attributes.value: 2021-04-06
attributes.tag: External Id
attributes.value: SAMEA8521034
attributes.tag: INSDC center alias
attributes.value: Mayo Clinic
attributes.tag: INSDC center name
attributes.value: Mayo Clinic
attributes.tag: INSDC first public
attributes.value: 2021-04-14T00:24:52Z
attributes.tag: INSDC last update
attributes.value: 2021-04-06T13:32:26Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-10321:PSN2
attributes.tag: age
attributes.value: 26
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: placental site nodule
attributes.tag: individual
attributes.value: PSN2
attributes.tag: organism part
attributes.value: uterus
attributes.tag: sample name
attributes.value: E-MTAB-10321:PSN2
attributes.tag: sex
attributes.value: female