description: Protocols: Out of 60 RNAs, 16 derived from FFPE sections (Formalin fixed paraffin embedded), 29 from frozen sections and 15 were previously extracted RNAs from MTC patients. 3 sera were obtained from whole blood samples by centrifugation at 1900 x g for 10 min at 4°C. The supernatant was further centrifuged at 16000 x g for 10 min at 4°C and stored in aliquots of 0.5 mL at -80°C until analysis. RNA isolations were performed from both FFPE and frozen sections using FFPE DNA Purification Kit (Cat. 47400, Norgen Biotek Corp) and RNA/DNA Purification Kit (Cat. 48700, Norgen Biotek Corp.) respectively, according to the manufacturers protocol.The extraction of total RNA from 200 µL of serum was performed within 1 year of storage at -80°C using miRNeasy Serum/Plasma Kit (Qiagen, Hilden, Germany) according to the manufacturers instructions. Small-RNA libraries were prepared with NEXTFLEX Small RNA-Seq Kit v3 (Cat NOVA-5132-06, Perkin Elmer) starting from 50ng of RNA as input.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-06-30
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-06-30
attributes.tag: External Id
attributes.value: SAMEA13774431
attributes.tag: INSDC center alias
attributes.value: "Laboratory of Molecular Medicine and Genomics, Department of Medicine, Surgery and Dentistry \""Scuola Medica Salernitana\"", University of Salerno"
attributes.tag: INSDC center name
attributes.value: "Laboratory of Molecular Medicine and Genomics, Department of Medicine, Surgery and Dentistry \""Scuola Medica Salernitana\"", University of Salerno"
attributes.tag: INSDC first public
attributes.value: 2022-06-30T00:14:52Z
attributes.tag: INSDC last update
attributes.value: 2022-06-30T00:14:52Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11598:Sample 20
attributes.tag: age
attributes.value: 68
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: medullary thyroid gland carcinoma
attributes.tag: individual
attributes.value: MTC_15
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: thyroid gland
attributes.tag: sample name
attributes.value: E-MTAB-11598:Sample 20
attributes.tag: sex
attributes.value: female
attributes.tag: specimen with known storage state
attributes.value: frozen specimen