description: Protocols: T-cells were obtained from bone marrow samples by isolation of mononuclear cells, using density gradient centrifugation, followed by immunomagnetic separation, with use of Human T Lymphocyte Enrichment Set-DM (Becton Dickinson) Total RNA was isolated using miRCURY RNA Isolation Kit Cell & Plant (Exiqon) or miRNeasy Kit (Qiagen). RNA isolates were DNase treated and purified with use of RNA Clean and Concentrator Kit (Zymo Research). Libraries were generated with Illumina TruSeq Stranded mRNA Prep Kit. Modified fragmentation conditions were used (90'C for 2 minutes)
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-06-30
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-06-30
attributes.tag: External Id
attributes.value: SAMEA14414549
attributes.tag: INSDC center alias
attributes.value: Institute of Human Genetics Polish Academy of Sciences
attributes.tag: INSDC center name
attributes.value: Institute of Human Genetics Polish Academy of Sciences
attributes.tag: INSDC first public
attributes.value: 2022-06-30T00:15:01Z
attributes.tag: INSDC last update
attributes.value: 2022-06-30T00:15:01Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11759:1414_PA_P3
attributes.tag: age
attributes.value: 8
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_type
attributes.value: T cell
attributes.tag: clinical information
attributes.value: prior to treatment
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: juvenile
attributes.tag: disease
attributes.value: T-cell acute lymphoblastic leukemia
attributes.tag: individual
attributes.value: 1414
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: bone marrow
attributes.tag: sample name
attributes.value: E-MTAB-11759:1414_PA_P3
attributes.tag: sex
attributes.value: female