description: Protocols: Tissues were captured from two patients, and stored at -80oC immediately. In brief, 10% whole cell lysates were saved as input after genomic DNA was broken into 200-500 bp by sonication. 1 μg IP grade antibodies of H3K4/9/27/36/79me3 (CST, Boston, USA) were incubated with the rest of the lysate overnight, followed by 2 h protein-A beads incubation at 4 °C for target protein pull down. The DNA pulled down or input DNA were repaired to 3'-dA overhang and added the ligated adapter.
attributes.tag: ENA first public
attributes.value: 2021-04-17
attributes.tag: ENA last update
attributes.value: 2021-04-07
attributes.tag: External Id
attributes.value: SAMEA8536122
attributes.tag: INSDC center alias
attributes.value: Department of Oral and Maxillofacial Surgery, College of Stomatology, the Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine
attributes.tag: INSDC center name
attributes.value: Department of Oral and Maxillofacial Surgery, College of Stomatology, the Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine
attributes.tag: INSDC first public
attributes.value: 2021-04-17T00:23:19Z
attributes.tag: INSDC last update
attributes.value: 2021-04-07T16:46:13Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-10340:H3K4me3_LG_NC1_1
attributes.tag: age
attributes.value: 52
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: pSS
attributes.tag: organism part
attributes.value: labial gland
attributes.tag: sample name
attributes.value: E-MTAB-10340:H3K4me3_LG_NC1_1
attributes.tag: sex
attributes.value: female