description: Protocols: Embryos from Insm1GFP.Cre/+; Neurod1Lacz/+ dbl het x dbl het (OR Insm1GFP.Cre/+; Paxfl/fl dbl het x dbl het) mouse matings were collected at embryonic day E15.5, where the presence of vaginal plug at noon was considered as E0.5. Embryos were genotyped by PCR and pancreata from Insm1GFP.Cre/+ controls and Insm1, Neurod1, or Pax6 knockout mouse embryos were dissected out for RNA islolation. Insm1GFP.Cre/+ and Neurod1LacZ/+ mice used for experiments were on CD-1 background, and Pax6fl/fl were on a C57Black/6J background. Insm1GFP.Cre/+ controls and Insm1-, Neurod1, and Pax6-knockout embryonic pancreata were used in the experiment. Total RNA was extracted from E15.5 mouse pancreatic tissue using Trizol LS and DNA-free RNA Kit (Zymo Research). cDNA libraries were constructed using Illumina library prep kit at Vanderbilt Technologies for Advanced Genomics.
attributes.tag: ENA first public
attributes.value: 2021-04-15
attributes.tag: ENA last update
attributes.value: 2021-03-23
attributes.tag: External Id
attributes.value: SAMEA8398372
attributes.tag: INSDC center alias
attributes.value: VANDERBILT UNIVERSITY
attributes.tag: INSDC center name
attributes.value: VANDERBILT UNIVERSITY
attributes.tag: INSDC first public
attributes.value: 2021-04-15T00:27:38Z
attributes.tag: INSDC last update
attributes.value: 2021-03-23T13:44:09Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-10262:Insm1-het_Pax6-null (1078-KD-1)
attributes.tag: age
attributes.value: 15.5
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: embryo stage
attributes.tag: genotype
attributes.value: Insm1GFP.Cre/+; Pax6fl/fl
attributes.tag: individual
attributes.value: 10
attributes.tag: organism part
attributes.value: endocrine pancreas
attributes.tag: sample name
attributes.value: E-MTAB-10262:Insm1-het_Pax6-null (1078-KD-1)
attributes.tag: strain
attributes.value: Mixed CD-1 x C57BL/6J