description: Protocols: Out of 60 RNAs, 19 derived from FFPE sections (Formalin fixed paraffin embedded), 21 from frozen sections and 20 were previously extracted RNAs from MTC patients. RNA isolations were performed from both FFPE and frozen sections using FFPE DNA Purification Kit (Cat. 47400, Norgen Biotek Corp) and RNA/DNA Purification Kit (Cat. 48700, Norgen Biotek Corp.) respectively, according to the manufacturers protocol. Libraries preparation for transcriptome analysis was performed employing the TruSeq RNA Exome kit (Cat. 20020189, Illumina) for FFPE samples and TruSeq Stranded Total RNA kit (Cat. 20020598, Illumina) for frozen samples, starting from 200ng and 400ng of RNA as input materials, respectively, according to manufacturers' guidelines.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-06-30
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-06-30
attributes.tag: External Id
attributes.value: SAMEA13774452
attributes.tag: INSDC center alias
attributes.value: "Laboratory of Molecular Medicine and Genomics, Department of Medicine, Surgery and Dentistry \""Scuola Medica Salernitana\"", University of Salerno"
attributes.tag: INSDC center name
attributes.value: "Laboratory of Molecular Medicine and Genomics, Department of Medicine, Surgery and Dentistry \""Scuola Medica Salernitana\"", University of Salerno"
attributes.tag: INSDC first public
attributes.value: 2022-06-30T00:14:53Z
attributes.tag: INSDC last update
attributes.value: 2022-06-30T00:14:53Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11599:Sample 14
attributes.tag: age
attributes.value: 75
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: digestive system neuroendocrine neoplasm
attributes.tag: individual
attributes.value: MET_3
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: liver
attributes.tag: sample name
attributes.value: E-MTAB-11599:Sample 14
attributes.tag: sex
attributes.value: male
attributes.tag: specimen with known storage state
attributes.value: paraffin specimen