description: chromosome-level assembly of genome from metagenomic biosample SAMN05421555
attributes.tag: 16S recovered
attributes.value: Yes
attributes.tag: 16S recovery software
attributes.value: barrnap:0.9
attributes.tag: ENA first public
attributes.value: 2022-02-22
attributes.tag: ENA last update
attributes.value: 2022-02-22
attributes.tag: External Id
attributes.value: SAMEA13058561
attributes.tag: INSDC center alias
attributes.value: ETH ZURICH
attributes.tag: INSDC center name
attributes.value: ETH ZURICH
attributes.tag: INSDC first public
attributes.value: 2022-02-22T16:51:25Z
attributes.tag: INSDC last update
attributes.value: 2022-02-22T16:51:25Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E.malaspinii_MAG_MP1648_PBLIHF
attributes.tag: assembly quality
attributes.value: Multiple fragments where gaps span repetitive regions. Presence of the 23S, 16S and 5S rRNA genes and at least 18 tRNAs.
attributes.tag: assembly software
attributes.value: flye;2.9;defaults
attributes.tag: collection date
attributes.value: 2011-04-28
attributes.tag: completeness approach
attributes.value: Average of checkm and anvi'o (marker-gene based) and contiguous chromosome-level assembly
attributes.tag: completeness score
attributes.value: 96.81
attributes.tag: completeness software
attributes.value: checkm and anvi'o
attributes.tag: contamination score
attributes.value: 0
attributes.tag: contamination screening input
attributes.value: contiguous chromosome-level assembly and phylogenetic inspection of duplicated marker genes
attributes.tag: environment (biome)
attributes.value: Ocean Water
attributes.tag: environment (feature)
attributes.value: Ocean water
attributes.tag: environment (material)
attributes.value: Ocean water
attributes.tag: feature prediction
attributes.value: prokka:1.4;Prodigal:002006;Aragorn:001002;barrnap:0.9;minced:0.4.2
attributes.tag: geographic location (country and/or sea)
attributes.value: Pacific Ocean
attributes.tag: geographic location (depth)
attributes.value: 4018
attributes.tag: geographic location (latitude)
attributes.value: -5.75
attributes.tag: geographic location (longitude)
attributes.value: -170.7407
attributes.tag: investigation type
attributes.value: metagenome
attributes.tag: isolation source
attributes.value: ocean water
attributes.tag: metagenomic source
attributes.value: marine metagenome
attributes.tag: nucleic acid amplification
attributes.value: PacBio kits SMRTbell gDNA Sample amplification kit (100-980-000)
attributes.tag: number of standard tRNAs extracted
attributes.value: 21
attributes.tag: project name
attributes.value: Biosynthetic potential of the global ocean microbiome
attributes.tag: reassembly post binning
attributes.value: No
attributes.tag: reference for biomaterial
attributes.value: https://doi.org/10.1038/s42003-021-02112-2
attributes.tag: relevant standard operating procedures
attributes.value: PacBio HiFi reads were quality controlled (pbclip) and mapped against a short-read metagenome assembled genome of the species to select for reads mapping with an identity >=99% over >=2kbp. The selected reads were assembled with Flye.
attributes.tag: sample derived from
attributes.value: SAMN05421555
attributes.tag: sample name
attributes.value: E.malaspinii_MAG_MP1648_PBLIHF
attributes.tag: sequencing method
attributes.value: PacBio HiFi
attributes.tag: size fraction selected
attributes.value: 0.8-20 micrometers
attributes.tag: tRNA extraction software
attributes.value: Aragorn:001002
attributes.tag: taxonomic classification
attributes.value: GTDBTk:v1.5.0, Database:r202, d__Bacteria;p__Eremiobacterota;c__Xenobia;o__Xenobiales;f__;g__;s__
attributes.tag: taxonomic identity marker
attributes.value: multi-gene approach (GTDBTk:v1.5.0)