description: Protocols: Neuro-2a (mouse neuroblastoma N2a cells) cells were trypsinised using 1x Trypsin-EDTA (Sigma 59428C, 0.25% trypsin, 0.02% EDTA) and neutralised by the addition of FBS (Hyclone). The cell lines Neuro-2a (mouse neuroblastoma N2a cells) was grown in DMEM (GIBCO) supplemented with 10% FBS (HyClone) and 1% antibiotics. DNA was purified first by Qiagen minElute PCR purification kit according to the manufacturer's instruction, then by AmupureXP beads with 0.5X upper cutoff and a 1.2X lower cutoff according to manufacturer's instruction. Libraries were constructed in the Illumina Nextera DNA Library preparation kit.
attributes.tag: ENA first public
attributes.value: 2021-03-01
attributes.tag: ENA last update
attributes.value: 2020-12-15
attributes.tag: External Id
attributes.value: SAMEA7722624
attributes.tag: INSDC center alias
attributes.value: Department of Biology Southern University of Science and Technology
attributes.tag: INSDC center name
attributes.value: Department of Biology Southern University of Science and Technology
attributes.tag: INSDC first public
attributes.value: 2021-03-01T12:18:28Z
attributes.tag: INSDC last update
attributes.value: 2020-12-15T13:25:07Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-9919:Control_rep3
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: Neuro-2a
attributes.tag: cell type
attributes.value: neuroblast
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: neuroblastoma
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: brain
attributes.tag: sample name
attributes.value: E-MTAB-9919:Control_rep3