description: Protocols: 3D in vitro models of patient derived colon cancer tumors were processed to single cells, labelled with PKH26 dye and separated by FACS into PKH26 Negative and PKH26 Positive cell fractions after 12 days in culture. Colon cancer PDOs were cultured in growth factor reduced Matrigel as previously described (Regan, J. L. et al. Non-Canonical Hedgehog Signaling Is a Positive Regulator of the WNT Pathway and Is Required for the Survival of Colon Cancer Stem Cells. Cell Rep. 21, 2813-2828 (2017). Cells were lysed in RLT buffer and processed for RNA using the RNeasy Mini Plus RNA extraction kit (Qiagen) Samples were processed using NuGEN's Ovation RNA-Seq System V2
attributes.tag: ENA first public
attributes.value: 2021-02-08
attributes.tag: ENA last update
attributes.value: 2020-12-11
attributes.tag: External Id
attributes.value: SAMEA7705376
attributes.tag: INSDC center alias
attributes.value: Charite - Universitatsmedizin Berlin
attributes.tag: INSDC center name
attributes.value: Charite - Universitatsmedizin Berlin
attributes.tag: INSDC first public
attributes.value: 2021-02-08T04:04:36Z
attributes.tag: INSDC last update
attributes.value: 2020-12-11T13:43:43Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-8924:39_249_CB_P_PKH26_POS_S44
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: intestinal epithelial cell
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: colon carcinoma
attributes.tag: disease staging
attributes.value: primary tumour
attributes.tag: fraction
attributes.value: PKH26 Positive
attributes.tag: growth condition
attributes.value: organoid culture
attributes.tag: individual
attributes.value: 249_CB_P
attributes.tag: organism part
attributes.value: colon
attributes.tag: sample name
attributes.value: E-MTAB-8924:39_249_CB_P_PKH26_POS_S44