description: Protocols: ATAC-seq was performed with 100,000 cells per treatment, cells were grown in DMEM containing 5% fetal bovine serum. U2OS-AR cells where grown to approximately 90% confluence according to standard protocols in DMEM medium with 5% FBS. Cells were treated for 4 hours with 5 nM R1881 or 0.1% DMSO as vehicle control ATAC-seq was performed with 100,000 cells per treatment according to the Omni-ATAC-seq protocol [PMID: 28846090]. DNA was extracted using the DNA Clean & Concentrator Kit (Zymo research #D4014). Library amplification involved 11 PCR cycles using the NEBNext High-Fidelity 2x PCR Master Mix (New England Labs, Cat #M0541).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2021-03-09T14:36:56Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-09-29T16:57:33Z
attributes.tag: External Id
attributes.value: SAMEA7364793
attributes.tag: INSDC center name
attributes.value: MPIMG
attributes.tag: INSDC first public
attributes.value: 2021-03-09T14:36:56Z
attributes.tag: INSDC last update
attributes.value: 2020-09-29T16:57:33Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-9606:ATAC-seq_R1881_4h
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: U2OS-AR
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: osteosarcoma
attributes.tag: organism part
attributes.value: bone
attributes.tag: phenotype
attributes.value: expression of androgen receptor
attributes.tag: sample name
attributes.value: E-MTAB-9606:ATAC-seq_R1881_4h
attributes.tag: scientific_name
attributes.value: Homo sapiens