description: Protocols: Mice were euthanized and lungs were harvested and digested. Lung single cell suspensions were prepared and CD45-CD31-CD326-CD140a+ cells were sorted, and ex vivo cultured for further experiments. Ex vivo cultured lung CD140a+ mesenchymal cells (fibroblasts) were stimulated with vehicle or IL-1β for 24 hours. Total RNA was isolated from lung CD140a+ mesenchymal cells (fibroblasts) using the miRNeasy Mini kit. Stranded libraries were prepared using the KAPA mRNA HyperPrep Kit: isolation of polyA containing mRNA using oligo-dT magnetic beads, RNA fragmentation, first and second strand cDNA synthesis, ligation of Illumina-specific adapters containing a unique barcode sequence for each library, and PCR amplification. Libraries were checked for quality and concentration using the DNA 1000 assay and quantitative PCR, following the manufacturers' instructions.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-07-19
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-07-19
attributes.tag: External Id
attributes.value: SAMEA110188282
attributes.tag: INSDC center alias
attributes.value: THE JACKSON LABORATORY
attributes.tag: INSDC center name
attributes.value: THE JACKSON LABORATORY
attributes.tag: INSDC first public
attributes.value: 2022-07-19T00:15:36Z
attributes.tag: INSDC last update
attributes.value: 2022-07-19T00:15:36Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11887:Sample 8
attributes.tag: age
attributes.value: 10
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_type
attributes.value: mesenchymal cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: immunophenotype
attributes.value: CD45-; CD31-; CD326-; CD140a+
attributes.tag: individual
attributes.value: 8
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: lung
attributes.tag: sample name
attributes.value: E-MTAB-11887:Sample 8
attributes.tag: sex
attributes.value: female
attributes.tag: stimulus
attributes.value: interleukin 1 beta
attributes.tag: strain
attributes.value: C57BL/6J