description: Protocols: Naïve C57BL/6J mice were euthanized, and lung was harvested and digested. Lung single cell suspension was prepared and stained with primary-fluorophore conjugated antibodies. Lung stromal cells (CD45- CD31- CD326-) were isolated by fluorescence-activated cell sorting. Cells capture, lysis and RNA extraction were performed using the 10x V3 Chromium technology. Around 12,000 cells were loaded in each lane of the 10x Chromium chip. Barcoding and library preparation were performed with the 10x Chromium system using version 3 chemistry, following the manufacturer's protocol (10x Genomics). cDNA was synthesized and amplified as per manufacturer's protocol and amplified to construct an Illumina sequencing library. cDNA and libraries were checked for quality on an Agilent 4200 TapeStation, quantified by KAPA qPCR.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-07-19
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-07-19
attributes.tag: External Id
attributes.value: SAMEA110187715
attributes.tag: INSDC center alias
attributes.value: THE JACKSON LABORATORY
attributes.tag: INSDC center name
attributes.value: THE JACKSON LABORATORY
attributes.tag: INSDC first public
attributes.value: 2022-07-19T00:15:36Z
attributes.tag: INSDC last update
attributes.value: 2022-07-19T00:15:36Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11879:Sample 1
attributes.tag: age
attributes.value: 10
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_type
attributes.value: stromal cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: immunophenotype
attributes.value: CD45-; CD31-; CD326-
attributes.tag: individual
attributes.value: 1
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: lung
attributes.tag: sample name
attributes.value: E-MTAB-11879:Sample 1
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: C57BL/6J