description: Using a two-plasmid CRISPR/Cas9-based gene editing approach, a conditional PfPKAc overexpression (OE) cassette (PfPKAc-GFP-glms) has been inserted into the non-essential glp3 locus (Pf3D7_0709200, chromosome 7) in Plasmodium falciparum strain NF54 parasites. The transgenic line has been cloned out and two clonal lines have been obtained (NF54/PKAc cOE M1 and M2). PfPKAc-GFP OE can be induced via removal of glucosamine from the culture medium. Induction of PfPKAc-GFP OE is lethal in both NF54/PKAc cOE M1 and M2 clones. After constant selection of parasite growth in absence of glucosamine, six independent parasite populations tolerant to PfPKAc-GFP OE were obtained and these survivor populations have been termed NF54/PKAc-cOE S1-S3 (derived from NF54/PKAc cOE M1) and NF54/PKAc-cOE S4-S6 (derived from NF54/PKAc cOE M2).
attributes.tag: ENA first public
attributes.value: 2021-05-04
attributes.tag: ENA last update
attributes.value: 2021-04-29
attributes.tag: External Id
attributes.value: SAMEA8658907
attributes.tag: INSDC center alias
attributes.value: Swiss Tropical and Public Health Institute
attributes.tag: INSDC center name
attributes.value: Swiss Tropical and Public Health Institute
attributes.tag: INSDC first public
attributes.value: 2021-05-04T00:42:32Z
attributes.tag: INSDC last update
attributes.value: 2021-04-29T21:12:17Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: SRA accession
attributes.value: ERS6343453
attributes.tag: Sample Name
attributes.value: NF54/PKAc cOE S1
attributes.tag: Submitter Id
attributes.value: NF54/PKAc cOE S1
attributes.tag: collecting institution
attributes.value: Swiss Tropical and Public Health Institute
attributes.tag: collector name
attributes.value: Eva Hitz
attributes.tag: common name
attributes.value: malaria parasite P. falciparum
attributes.tag: dev stage
attributes.value: intra-erythrocytic parasites
attributes.tag: geographic location (country and/or sea)
attributes.value: Netherlands
attributes.tag: isolate
attributes.value: NF54
attributes.tag: title
attributes.value: Whole genome sequence of the transgenic NF54/PKAc cOE S1 survivor