description: Protocols: We grew MCF-10-2A TetOn-CENPA-FLAG-HA cells expressing either empty vector (p53-WT) or dominant-negative p53 (p53-DN) with 0X Dox (no Dox), 1X Dox (10 ng/ml), or 10X Dox (100 ng/ml) for 24h. At time 0, we irradiated one set of cells by X-ray generator (4gy) while a control set remained un-irradiated (0gy). 6h later, we extracted RNA for RNA-seq. All conditions tested in duplicate. We extracted RNA directly from culture dishes using the Qiagen RNeasy Mini kit according to the manufacturer's instructions and confirmed the quality and quantity of the RNA by TapeStation and NanoDrop. mRNA library preparation was performed by the Next Generation Sequencing (NGS) platform at Institut Curie using the Illumina TruSeq Stranded mRNA kit
attributes.tag: ENA first public
attributes.value: 2021-03-01
attributes.tag: ENA last update
attributes.value: 2020-12-09
attributes.tag: External Id
attributes.value: SAMEA7699766
attributes.tag: INSDC center alias
attributes.value: Nuclear Dynamics Unit UMR3664 CNRS/IC Institut Curie - Centre de Recherche
attributes.tag: INSDC center name
attributes.value: Nuclear Dynamics Unit UMR3664 CNRS/IC Institut Curie - Centre de Recherche
attributes.tag: INSDC first public
attributes.value: 2021-03-01T12:18:27Z
attributes.tag: INSDC last update
attributes.value: 2020-12-09T16:42:58Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-9867:D092T125
attributes.tag: batch
attributes.value: 2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: MCF-10-2A
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: breast fibrocystic disease
attributes.tag: genotype
attributes.value: p53-WT TetOn-CENPA-FLAG-HA
attributes.tag: organism part
attributes.value: mammary gland
attributes.tag: phenotype
attributes.value: wild type phenotype
attributes.tag: sample name
attributes.value: E-MTAB-9867:D092T125