description: Protocols: The flag leaf on the first tiller of each plant was inoculated with urediniospores of Pt race MDT-MR suspended in mineral oil, or mineral oil alone (Mock-inoculation). Pt- and Mock-inoculated plants were collected at 0, 6, 12 and 24 hours after inoculation (hai), the leaf tissue being placed immediately into liquid nitrogen. Individual plants of the Brazilian wheat cv. Toropi (seed obtained from Embrapa-Wheat, Brazil) were grown to first flag leaf emergence under a 14 h light/20 ºC and 10 h dark/16 ºC growing cycle, at 80% humidity. Each plant was grown in a soil substrate (rice husk, peat and humus) and vermiculite mix in a 1 liter pot. The flag leaf on the first tiller of each plant was inoculated with urediniospores of Pt race MDT-MR suspended in mineral oil, or mineral oil alone (Mock-inoculation). Pt- and Mock-inoculated plants were collected at 0, 6, 12 and 24 hours after inoculation (hai), the leaf tissue being placed immediately into liquid nitrogen. RNA was extracted from flag leaves using RNAeasy Plant Mini kit (Qiagen) and purified using the Turbo DNA-freeTM kit (Invitrogen). For each time point (0, 6, 12, 24 hai) RNA from three Pt and three Mock-inoculated flag leaves was sent to the Earlham Institute (formerly The Genome Analyses Centre), Norwich, UK for library construction and paired-end sequencing.
attributes.tag: ENA first public
attributes.value: 2021-03-01
attributes.tag: ENA last update
attributes.value: 2020-11-20
attributes.tag: External Id
attributes.value: SAMEA7591615
attributes.tag: INSDC center alias
attributes.value: National Inst of Agri Botany
attributes.tag: INSDC center name
attributes.value: National Inst of Agri Botany
attributes.tag: INSDC first public
attributes.value: 2021-03-01T12:18:17Z
attributes.tag: INSDC last update
attributes.value: 2020-11-20T15:53:25Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-9791:6hpi_inoc_rep3
attributes.tag: age
attributes.value: 60
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: bread wheat
attributes.tag: cultivar
attributes.value: Toropi
attributes.tag: developmental stage
attributes.value: first flag leaf emergence
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: leaf
attributes.tag: sample name
attributes.value: E-MTAB-9791:6hpi_inoc_rep3