description: Protocols: Untreated and treated samples were harvested by centrifugation. Saccharomyces cerevisiae wild-type W303 (MATa ura3-52 leu2-3 leu2-112 trp1-1 ade2-1 his3-11 can1-100) and tsa1D (tsa1::LEU2) were grown in triplicates at 30oC shaking till mid exponential phase in minimal SD medium (0.17% w/v yeast nitrogen base without amino acids with 0.5% w/v ammonium sulfate, 2% w/v glucose supplemented with appropriate amino acids and bases). To induce protein misfolding azetidine-2-carboxylic acid (AZC) (5 mM) was added for two hours. Untreated and treated samples were harvested and total RNA was isolated using Trizol (Life Technologies, Carlsbad, CA) and quantified using a Nanodrop 8000 spectrophotometer (Thermo Fisher Scientific, Waltham, MA). rRNA was depleted using the RibominusTM Eukaryote Kit for RNA-Seq (Life Technologies, Carlsbad, CA). Truseq stranded mRNA Assay.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2021-03-11T04:06:35Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-07-15T19:21:48Z
attributes.tag: External Id
attributes.value: SAMEA7062471
attributes.tag: INSDC center name
attributes.value: UNIVERSITY OF MANCHESTER
attributes.tag: INSDC first public
attributes.value: 2021-03-11T04:06:35Z
attributes.tag: INSDC last update
attributes.value: 2020-07-15T19:21:48Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-9355:Sample 11
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: baker's yeast
attributes.tag: genotype
attributes.value: Tsa1 deletion
attributes.tag: sample name
attributes.value: E-MTAB-9355:Sample 11
attributes.tag: scientific_name
attributes.value: Saccharomyces cerevisiae
attributes.tag: strain
attributes.value: W303