description: Protocols: Embryos from Zfp800 het x het mouse maitings were collected at embryonic day E18.5. Embryos were genotyped by PCR and pancreata from wild type and Zfp800 knockout mouse embryos were dissected out for RNA islolation. All mice used for experiments were on C57Black/6J background. Wild type and Zfp800 knockout embryonic pancreata were used in the experiment. Total RNA was extracted from mouse pancreatic tissue using the Maxwell simplyRNA kit (Promega). cDNA libraries were constructed using Illumina library prep kit at Novogen.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2021-03-02T00:47:26Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-09-09T15:34:37Z
attributes.tag: External Id
attributes.value: SAMEA7296885
attributes.tag: INSDC center name
attributes.value: VANDERBILT UNIVERSITY
attributes.tag: INSDC first public
attributes.value: 2021-03-02T00:47:26Z
attributes.tag: INSDC last update
attributes.value: 2020-09-09T15:34:37Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-9538:2142-OSM-1
attributes.tag: age
attributes.value: embryonic day 18.5
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: embryo
attributes.tag: genotype
attributes.value: Zfp800 -/-
attributes.tag: individual
attributes.value: 1
attributes.tag: organism part
attributes.value: endocrine pancreas
attributes.tag: sample name
attributes.value: E-MTAB-9538:2142-OSM-1
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: C57BL/6J