description: Protocols: Heterogeneous spheres were prepared from primary human fibroblasts and G361 melanoma cell line (commercially available; CVCL_1220). Fibroblasts were isolated from photo-aged skin of an adult and from juvenile photo-protective skin of child. Heterogeneous spheres were washed in PBS and digested in 0.25% trypsin + EDTA solution (1:1). Cell suspensions were inoculated in DMEM + 10% fetal bovine serum for 72 hours in hanging drops. Heterogeneous spheres were cultured for additional 24 hours in non-adhesive culture dishes. Immediately after tissue dissociation, single cell libraries were prepared on the 10x Chromium with a target of 4,000 cells. Chromium Single Cell 3ʹ GEM, Library & Gel Bead Kit v3, 4 rxns PN-1000092 was used. Immediately after tissue dissociation, single cell libraries were prepared on the 10x Chromium with a target of 4,000 cells. Chromium Single Cell 3ʹ GEM, Library & Gel Bead Kit v3, 4 rxns PN-1000092 was used.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2021-03-01T12:39:56Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-06-13T23:17:48Z
attributes.tag: External Id
attributes.value: SAMEA6952712
attributes.tag: INSDC center name
attributes.value: Institute of Molecular Genetics of the Czech Academy of Sciences
attributes.tag: INSDC first public
attributes.value: 2021-03-01T12:39:56Z
attributes.tag: INSDC last update
attributes.value: 2020-06-13T23:17:48Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-9216:PDF_1
attributes.tag: age
attributes.value: 31
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: fibroblast
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: photodamage
attributes.tag: ethnic group
attributes.value: Caucasian
attributes.tag: growth condition
attributes.value: spheroids with melanoma cell line G361
attributes.tag: organism part
attributes.value: dermis
attributes.tag: sample name
attributes.value: E-MTAB-9216:PDF_1
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: male