description: Protocols: Plants were harvested directly after the 4 h treatment. The leaf material was frozen in liquid nitrogen and stored at -80 °C. Plants were grown for 4 weeks under short day (10 h light/14 h dark and 20 °C/16 °C, respectively). The relative humidity during the day and night was 50 %. Four-week-old plants were transferred at 11 am (5 h after turning on the light) for 4 h either to dark (0 µE, 22 °C) or low light (100-130 µE, 22 °C). Around 100 mg of Arabidopsis leaf tissue was ground to a fine powder, followed by RNA extraction using the RNeasy Plant Mini Kit (Qiagen) according to the manufacturer's instructions. RNA concentration and quality were determined by NanoDrop 1000. Sequencing libraries were generated from poly(A)-enriched RNA using the NEBNext Ultra II Directional RNA Library Prep kit (New England Biolabs) according to the manufacturer's instructions.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2021-03-01T12:17:02Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-07-03T21:30:55Z
attributes.tag: External Id
attributes.value: SAMEA7039533
attributes.tag: INSDC center name
attributes.value: Institute of Biochemical Plant Pathology Helmholtz Zentrum Munchen Deutsches Forschungszentrum fur Gesundheit und Umwelt (GmbH)
attributes.tag: INSDC first public
attributes.value: 2021-03-01T12:17:02Z
attributes.tag: INSDC last update
attributes.value: 2020-07-03T21:30:55Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-9301:Sample 24
attributes.tag: age
attributes.value: 4
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: thale cress
attributes.tag: developmental stage
attributes.value: rosette growth stage
attributes.tag: ecotype
attributes.value: Col-0
attributes.tag: environmental stress
attributes.value: low light regimen
attributes.tag: genotype
attributes.value: hda6
attributes.tag: organism part
attributes.value: rosette leaf
attributes.tag: replicate
attributes.value: 4
attributes.tag: sample name
attributes.value: E-MTAB-9301:Sample 24
attributes.tag: scientific_name
attributes.value: Arabidopsis thaliana