description: Protocols: For adult samples, fresh mucosal tissue were excised within 60 minutes of circulatory arrest and colon tissue preserved in University of Wisconsin (UW) organ preservation solution (Belzer UW® Cold Storage Solution, Bridge to Life, USA) and mLN stored in saline at 4°C until processing. All tissues were processed immediately after isolation using consistent protocols with variation in enzymatic digestion strength (dx.doi.org/10.17504/protocols.io.q3xdypn). Cells were counted using NucleoCounter NC-200 and Via1-Cassette and diluted in 0.04% BSA in PBS to a concentration for capture of to attain 8,000-10,000 cells per well according the the 10x Genomics manufacturers protocol. Cells were loaded into the 10x Chromium Controller according to the manufacturer's protocol at a concentration that would attain between 8,000-10,000 cells/chip position. 10x Genomics VDJ libraries were generated from the 5' 10x Genomics Chromium cDNA libraries as detailed in the manufacturer's protocol.
attributes.tag: ENA first public
attributes.value: 2021-08-19
attributes.tag: ENA last update
attributes.value: 2021-08-19
attributes.tag: External Id
attributes.value: SAMEA9697388
attributes.tag: INSDC center alias
attributes.value: Wellcome Sanger Institute
attributes.tag: INSDC center name
attributes.value: Wellcome Sanger Institute
attributes.tag: INSDC first public
attributes.value: 2021-08-19T12:17:04Z
attributes.tag: INSDC last update
attributes.value: 2021-08-19T12:17:04Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-9532_2:Human_colon_16S8157860
attributes.tag: age
attributes.value: 70 to 75
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: T cell
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: individual
attributes.value: 386C
attributes.tag: organism part
attributes.value: transverse colon
attributes.tag: sample name
attributes.value: E-MTAB-9532_2:Human_colon_16S8157860
attributes.tag: sex
attributes.value: female