description: Protocols: WT and Ptgs2-cKO naïve mice were euthanized and lungs were harvested and digested. Lung single cell suspensions were prepared and CD11b+ DCs, CD103+ DCs and monocytes were sorted for RNA-seq. Cells were lysed and homogenized in TRIzol Reagent, then RNA was isolated using the miRNeasy Micro kit, according to manufacturers' protocols, including the optional DNase digest step. RNA concentration and quality were assessed using the Date Extracted (initials) spectrophotometer and the Nanodrop 2000 Assay. Libraries were constructed using the SMARTer Stranded Total RNA-Seq Kit v2-Pico, according to the manufacturer's protocol. Briefly, the protocol entails RNA fragmentation and cDNA synthesis, addition of Illumina-specific adapters containing a unique barcode sequence for each library, cleavage of ribosomal cDNA with Takara's ZapR v2 and R-Probes v2, and PCR amplification of uncleaved fragments. The quality and concentration of the libraries were assessed using the D5000 ScreenTape and Qubit dsDNA HS Assay, respectively, according to the manufacturers' instructions.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-07-19
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-07-19
attributes.tag: External Id
attributes.value: SAMEA110191374
attributes.tag: INSDC center alias
attributes.value: The Jackson Laboratory
attributes.tag: INSDC center name
attributes.value: the jackson laboratory
attributes.tag: INSDC first public
attributes.value: 2022-07-19T00:15:36Z
attributes.tag: INSDC last update
attributes.value: 2022-07-19T00:15:36Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11888:KO-Mo-1
attributes.tag: age
attributes.value: 10
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_type
attributes.value: monocyte
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: Ptgs2-cKO
attributes.tag: individual
attributes.value: 11
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: lung
attributes.tag: sample name
attributes.value: E-MTAB-11888:KO-Mo-1
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: C57BL/6