description: Protocols: The RNA-seq library preparation was performed for H357 CisR Nt Sh and H357 CisR CMTM6 shRNA treated with vehicle control (UT) and cisplatin (10 μM) (CisT) for 24 h. The cells preserved in LBP lysis buffer were first taken out from -80°C and thawed by placing the plates/tubes on ice. Total RNA was isolated using NucleoSpin RNA Plus kit (Macherey-Nagel) according to manufacturer's protocol. RNA concentration was estimated by Nanodrop (Thermo) and then 2ug of total RNA was used to prepare library. For RNA-seq library preparation 2 μg of total RNA was used to isolate mRNA through magnetic beads using mRNA isolation kit (PolyA mRNA isolation Module, NEB) followed by RNA-seq library preparation using mRNA library preparation kit (NEB) strictly following the vendor recommended protocol.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2021-02-08T17:06:27Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-08-10T15:48:24Z
attributes.tag: External Id
attributes.value: SAMEA7171690
attributes.tag: INSDC center name
attributes.value: Institute of Life Sciences, Bhubaneswar
attributes.tag: INSDC first public
attributes.value: 2021-02-08T17:06:27Z
attributes.tag: INSDC last update
attributes.value: 2020-08-10T15:48:24Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-9424:H357CisR_NtSh_UT_Rep2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: H357
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: tongue squamous cell carcinoma
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: replicate
attributes.value: Rep2
attributes.tag: sample name
attributes.value: E-MTAB-9424:H357CisR_NtSh_UT_Rep2
attributes.tag: scientific_name
attributes.value: Homo sapiens