description: Protocols: RNA was isolated from 1.2 million cells using the RNeasy kit from Qiagen. U2OS-AR cells where grown to approximately 90% confluence according to standard protocols in DMEM medium with 5% FBS. Cells were treated for 24 hours with 5 nM R1881 or 0.1% DMSO as vehicle control. Prior to reverse transcription, poly adenylated RNA was isolated using oligo d(T) beads. Sequencing libraries were prepared using the TruSeq RNA library Prep Kit (Illumina).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2021-03-09T14:36:56Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-10-01T11:34:24Z
attributes.tag: External Id
attributes.value: SAMEA7370811
attributes.tag: INSDC center name
attributes.value: MPIMG
attributes.tag: INSDC first public
attributes.value: 2021-03-09T14:36:56Z
attributes.tag: INSDC last update
attributes.value: 2020-10-01T11:34:24Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-9622:RNA-seq_dmso_24h_rep1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: U2OS-AR
attributes.tag: cell type
attributes.value: epithelial cell
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: osteosarcoma
attributes.tag: organism part
attributes.value: bone
attributes.tag: phenotype
attributes.value: expression of androgen receptor
attributes.tag: sample name
attributes.value: E-MTAB-9622:RNA-seq_dmso_24h_rep1
attributes.tag: scientific_name
attributes.value: Homo sapiens