description: Protocols: Cells were collected in exponential phase (OD=0.2 at 600nm) or transition to stationary phase (OD=0.9-1.2 for glucose, OD=1.9-2.2 for glucose+PGA,at 600nm). Growth in minimal medium M63 supplemented with sucrose (0.2% wt/vol) and 0.2% PGA (polygalacturonate) Treated samples were subjected to 15 min novobiocin shock (100 ug/ml) Total RNA from each D. dadantii sample was isolated using the frozen acid phenol method (Maes et Messens 1992), and submitted to Vertis Biotechnologie AG for ribosomal depletion (Illumina RiboZero kit) and RNA fragmentation. Strand-specific cDNA libraries were constructed by Vertis Biotechnologie AG.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2021-02-11T04:13:56Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-05-04T23:13:32Z
attributes.tag: External Id
attributes.value: SAMEA6813502
attributes.tag: INSDC center name
attributes.value: INSA Lyon
attributes.tag: INSDC first public
attributes.value: 2021-02-11T04:13:56Z
attributes.tag: INSDC last update
attributes.value: 2020-05-04T23:13:32Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-9025:ihfA_PGA_stat_nov_1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: growth condition
attributes.value: M63+G+PGA, transition to stationary phase
attributes.tag: sample name
attributes.value: E-MTAB-9025:ihfA_PGA_stat_nov_1
attributes.tag: scientific_name
attributes.value: Dickeya dadantii
attributes.tag: strain
attributes.value: 3937