description: Protocols: Whole blood was collected at the time of recruitment into PAXgene blood miRNA tubes (PreAnalytiX), frozen, and total RNA (including RNA > 18 nucleotides) was isolated according to the manufacturer's instructions (Qiagen). RNA samples were stored at −80°C, before undergoing an additional DNAse treatment using an RNA clean & concentrator kit (Zymo Research). Input material was normalised and strand specific library preparation was completed using NEBNext® Ultra™ II mRNA kit (NEB) and NEB rRNA/globin depletion probes following manufacturer's instructions. Libraries were on a Tetrad (Bio-Rad) using in-house unique dual indexing primers. Individual libraries were normalised using Qubit and pooled together. The pooled library was diluted to ~10 nM for storage and denatured and further diluted prior to loading on the sequencer.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-07-19
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-07-19
attributes.tag: External Id
attributes.value: SAMEA10105117
attributes.tag: INSDC center alias
attributes.value: Section of Paediatric Infectious Disease, Faculty of Medicine, Imperial College London
attributes.tag: INSDC center name
attributes.value: Section of Paediatric Infectious Disease, Faculty of Medicine, Imperial College London
attributes.tag: INSDC first public
attributes.value: 2022-07-19T20:17:28Z
attributes.tag: INSDC last update
attributes.value: 2022-07-19T20:17:28Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-10926:Sample 46
attributes.tag: age
attributes.value: 55
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: clinical history
attributes.value: no steroid, monoclonal antibody or interferon treatment
attributes.tag: clinical information
attributes.value: WHO severity score 6
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: COVID-19
attributes.tag: disease staging
attributes.value: severe disease severity
attributes.tag: individual
attributes.value: P014
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: blood
attributes.tag: sample name
attributes.value: E-MTAB-10926:Sample 46
attributes.tag: sex
attributes.value: male