description: Chn WT ChIPseq replicate 7
attributes.tag: IP_input
attributes.value: IP
attributes.tag: replicate
attributes.value: 2
attributes.tag: breed
attributes.value: Canton
attributes.tag: age
attributes.value: 2-16h
attributes.tag: sex
attributes.value: pooled male and female
attributes.tag: tissue
attributes.value: whole embryo
attributes.tag: genotype
attributes.value: WT
attributes.tag: growth_protocol
attributes.value: Fly stocks were maintained with standard protocols. Embryos were collected from food plates (corn+yeast extract+sugar+agar) at 25C and used directly for experiments.
attributes.tag: treatment
attributes.value: Embryos were cross linked in 1.8% formaldehyde, chromatin was sonicated to an average size of ∼500 bp (whole cell extract (WCE)), and 300 μl WCE from ∼120 mg embryos was incubated with protein A–conjugated Dynabeads (Invitrogen 100-02D), coated with antibodies against D. melanogaster Chn, Med-19 (Custom antibodies raised the lab) and histones marks (H3K27Ac, H3K3me1 and H3K4me3). For input controls, 50 μl WCE was used. Preparations of DNA libraries for single-end sequencing were done according to instructions from Illumina.
attributes.tag: BioSampleModel
attributes.value: Model organism or animal