description: Protocols: Participants underwent weekly evaluation using a questionnaire and biological sample collection for up to 16 weeks when attending for work and self-declared as fit to attend work at each visit, with further follow up samples collected at 24 weeks. At each visit, 9mL of whole blood was taken in Tempus Blood RNA Tubes. High quality, total RNA was extracted using Tempus Spin RNA Isolation Kit (Ambion; Life Technologies). Residual genomic DNA and globin mRNA were then removed from the samples using TURBO DNA-free Kit (Ambion; Life Technologies) and GLOBINclear Kit (Life Technologies), respectively. Sample QC was performed on Agilent's TapeStation instrument. Total RNA samples were submitted to University College London's Pathogen Genomics Unit for library preparation and sequencing. cDNA libraries were generated using Roche's KAPA mRNA HyperPrep kit according to the manufacturer's guidelines with PolyA selection of mRNA.
attributes.tag: ENA first public
attributes.value: 2021-03-01
attributes.tag: ENA last update
attributes.value: 2021-01-18
attributes.tag: External Id
attributes.value: SAMEA7833011
attributes.tag: INSDC center alias
attributes.value: Division of Infection and Immunity, University College London, London, UK
attributes.tag: INSDC center name
attributes.value: Division of Infection and Immunity, University College London, London, UK
attributes.tag: INSDC first public
attributes.value: 2021-03-01T12:18:50Z
attributes.tag: INSDC last update
attributes.value: 2021-01-18T16:35:15Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-10022:HCW_0146_FUP2
attributes.tag: age
attributes.value: 28
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: class
attributes.value: PCR-positive_+2
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: SARS-CoV-2 infection
attributes.tag: ethnic group
attributes.value: White
attributes.tag: individual
attributes.value: Barts_146
attributes.tag: organism part
attributes.value: blood
attributes.tag: sample name
attributes.value: E-MTAB-10022:HCW_0146_FUP2
attributes.tag: sex
attributes.value: female