description: Protocols: Small intestinal organoid cultures were growing in 24-well plates SI organoids were growing in matrigel with Intesticult medium and they were split at least once before the experiment addition of 40ng/ml of recombinant mTNF in the culture medium Strand-specific sequencing libraries were prepared with the TruSeq stranded Total RNA kit (Illumina) from 1 µg total RNA of each sample and sequenced on an Illumina HiSeq2000 (100-nucleotide paired-end reads). RNA extraction and purification from enteroids was performed using the RNeasy mini-kit (Qiagen), as per manufacturer instructions. Strand-specific sequencing libraries were prepared with the TruSeq stranded Total RNA kit (Illumina)
attributes.tag: ENA first public
attributes.value: 2021-05-26
attributes.tag: ENA last update
attributes.value: 2021-05-26
attributes.tag: External Id
attributes.value: SAMEA8464838
attributes.tag: INSDC center alias
attributes.value: Biology Department, College of Science, UAE University
attributes.tag: INSDC center name
attributes.value: Biology Department, College of Science, UAE University
attributes.tag: INSDC first public
attributes.value: 2021-05-26T12:28:17Z
attributes.tag: INSDC last update
attributes.value: 2021-05-26T12:28:17Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-10217:D4566
attributes.tag: age
attributes.value: 6
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: Il10 -/-
attributes.tag: growth condition
attributes.value: organoid culture
attributes.tag: organism part
attributes.value: small intestine
attributes.tag: sample name
attributes.value: E-MTAB-10217:D4566
attributes.tag: sampling site
attributes.value: proximal
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: C57BL/6J