description: Neocalanus flemingeri adult females were collected from depth on September 2016 in Prince William Sound during the fall cruise of the Seward Line Long-term Observation Program (LTOP). Females were kept in laboratory and embryos were collected. When the NII and NIII stages were reached individuals were incubated for two days with FOOD and NO FOOD treatment. At 24 hr, nauplii were checked under the microscope to assess survival. At 2 days, individual nauplii were RNA exacted and processed for RNASeq.
attributes.tag: isolate
attributes.value: Offspring from field collected females
attributes.tag: isolation_source
attributes.value: Females were incubated in laboratory and embryos were maintained in the laboratory until the NII and NIII stage was reached
attributes.tag: collection_date
attributes.value: not collected
attributes.tag: geo_loc_name
attributes.value: USA: Alaska, Prince William Sound
attributes.tag: tissue
attributes.value: whole organism
attributes.tag: dev_stage
attributes.value: Nauplius stage NII/NIII
attributes.tag: BioSampleModel
attributes.value: Invertebrate