description: Protocols: Single cell suspension of skin, blood, intestine, bone marrow and gingiva were sorted by flow cytometry. Monocytes were identified as CD45+ Lin(CD3ε, TCR-β, CD19, B220, NK1.1, Ter119, Siglec F, Ly6G)- CD11b+ Ly6Chi CD64- MHCII-. Total RNA from ~700-40,000 FACS sorted monocytes was isolated using the Single Cell RNA Purification Kit (Norgen Biotek Corp). RNA was amplified with the SMART-Seq V3 Ultra Low Input RNA Kit (CloneTech)
attributes.tag: ENA first public
attributes.value: 2021-02-05
attributes.tag: ENA last update
attributes.value: 2021-01-26
attributes.tag: External Id
attributes.value: SAMEA7871865
attributes.tag: INSDC center alias
attributes.value: UNIVERSITY OF MANCHESTER
attributes.tag: INSDC center name
attributes.value: UNIVERSITY OF MANCHESTER
attributes.tag: INSDC first public
attributes.value: 2021-02-05T04:07:04Z
attributes.tag: INSDC last update
attributes.value: 2021-01-26T13:55:20Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-10039:Sample 11
attributes.tag: age
attributes.value: 8 to 16
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: monocyte
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: immunophenotype
attributes.value: CD45+ Lin(CD3, TCR-, CD19, B220, NK1.1, Ter119, Siglec F, Ly6G)- CD11b+ Ly6Chi CD64- MHCII-
attributes.tag: individual
attributes.value: 11
attributes.tag: organism part
attributes.value: blood
attributes.tag: sample name
attributes.value: E-MTAB-10039:Sample 11
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: C57BL/6J