description: Protocols: The experiments were performed in August 2019 on the Norwegian Nansen Legacy project cruise to the Northern Barents Sea. Polar cod were collected by trawling (78.7°N, 34.0°E) and kept in a 500 L tank with running seawater at the ambient seawater temperature outdoor on the deck of the cruise ship in the Northern Barents Sea. Female polar cod (total 5 fish) with average body and liver weight (Mean  SD) of 35.8  7.8 and 3.2  1.2 g, respectively were used in this experiment. Polar cod PCLS preparation was performed by using protocols previously described for Atlantic cod (Eide et al., 2014; Yadetie et al., 2018). The liver was sliced using Leica vibrating blade microtome VT1200 (Leica, Wetzlar, Germany). Slices (250 μm thick strips, about 4 x 4 mm) were cultured in the culture medium (Leibowitz-15 medium, Life Technologies™ Gibco®, Paisley, UK) supplemented with 10% charcoal-stripped fetal bovine serum and 1% penicillin-streptomycin-amphotericin (10,000 U/mL potassium penicillin, 10,000 μg/mL streptomycin and 25 μg/mL amphotericin B; Sigma-Aldrich) in the presence of DMSO vehicle control, BaP alone (0.1, 1, and 10 µM), or in combination with low concentrations of EE2 (5 nM), at 6°C with horizontal shaking (50 rpm) for 72 hours. Liver slices from a total of 5 female fish (biological replicates n = 5) were distributed in each group (paired sample design). After 72 hours in culture, slices were collected and snap-frozen in liquid nitrogen and stored at -80 °C until further processing. Total RNA was isolated from frozen slices using TRI Reagent according to the manufacturer's protocol (Sigma-Aldrich, St. Louis, MO, USA). RNA concentration and quality were assessed using NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA) and Agilent 2100 bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). All the RNA samples had RNA integrity number (RIN) values > 9. Sequenced by Novogene on Illumina NovaSeq 6000 System
attributes.tag: ENA first public
attributes.value: 2021-05-26
attributes.tag: ENA last update
attributes.value: 2021-05-26
attributes.tag: External Id
attributes.value: SAMEA8800045
attributes.tag: INSDC center alias
attributes.value: Department of Biological Sciences University of Bergen, Bergen, Norway
attributes.tag: INSDC center name
attributes.value: Department of Biological Sciences University of Bergen, Bergen, Norway
attributes.tag: INSDC first public
attributes.value: 2021-05-26T04:19:51Z
attributes.tag: INSDC last update
attributes.value: 2021-05-26T04:19:51Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-10509:Liver-slice_Fish_2_BaP+EE2_2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: individual
attributes.value: Fish 2
attributes.tag: organism part
attributes.value: liver
attributes.tag: replicate
attributes.value: 2
attributes.tag: sample name
attributes.value: E-MTAB-10509:Liver-slice_Fish_2_BaP+EE2_2
attributes.tag: sex
attributes.value: female