description: Treatment calf = normal diet and a mix of 9,10-anthraquinone=500mg and chloroform=50mg per kg of feed
attributes.tag: ENA first public
attributes.value: 2021-05-26
attributes.tag: ENA last update
attributes.value: 2021-05-26
attributes.tag: External Id
attributes.value: SAMEA7018944
attributes.tag: INSDC center alias
attributes.value: AGR
attributes.tag: INSDC center name
attributes.value: AgResearch Ltd, New Zealand
attributes.tag: INSDC first public
attributes.value: 2021-05-26T04:19:36Z
attributes.tag: INSDC last update
attributes.value: 2021-05-26T04:19:36Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: ELN02.Final.Pool.001.S98
attributes.tag: collection date
attributes.value: 2014-05-28
attributes.tag: environment (biome)
attributes.value: Bovine Gastrointestinal Track
attributes.tag: environment (feature)
attributes.value: Rumen
attributes.tag: environment (material)
attributes.value: Rumen contents
attributes.tag: geographic location (country and/or sea)
attributes.value: New Zealand
attributes.tag: geographic location (latitude)
attributes.value: -40.37906
attributes.tag: geographic location (longitude)
attributes.value: 175.610684
attributes.tag: host age
attributes.value: 10
attributes.tag: host common name
attributes.value: Calf
attributes.tag: host diet
attributes.value: Concentrate starter and total mixed ration
attributes.tag: host sex
attributes.value: female
attributes.tag: host subject id
attributes.value: 308
attributes.tag: host-associated environmental package
attributes.value: host-associated
attributes.tag: investigation type
attributes.value: metagenome
attributes.tag: nucleic acid amplification
attributes.value: Kittelmann, S., H. Seedorf, W. A. Walters, J. C. Clemente, R. Knight, J. I. Gordon, and P. H. Janssen. 2013. Simultaneous amplicon sequencing to explore co-occurrence patterns of bacterial, archaeal and eukaryotic microorganisms in rumen microbial communities. PloS one 8(2):e47879.
attributes.tag: nucleic acid extraction
attributes.value: Henderson, G., F. Cox, S. Kittelmann, V. H. Miri, M. Zethof, S. J. Noel, G. C. Waghorn, and P. H. Janssen. 2013. Effect of DNA extraction methods and sampling techniques on the apparent structure of cow and sheep rumen microbial communities. PloS one 8(9):e74787.
attributes.tag: pcr conditions
attributes.value: initial denaturation=95degC_2.0min; a defined number of cycles (30 for bacteria; 35 for archaea) of denaturation=95degC_20sec, annealing=52degC_20sec, and elongation = 65degC_ min; final extension=65degC_7min
attributes.tag: project name
attributes.value: Early Life Nutrition 02
attributes.tag: sample collection device or method
attributes.value: stomach tubing
attributes.tag: sample name
attributes.value: ELN02.Final.Pool.001.S98
attributes.tag: sample storage temperature
attributes.value: -20
attributes.tag: sample volume or weight for DNA extraction
attributes.value: 0.2
attributes.tag: sequencing method
attributes.value: Illumina MiSeq