description: S6-Nov1 technical replicate 1 Skudai river
attributes.tag: ENA first public
attributes.value: 2021-03-01
attributes.tag: ENA last update
attributes.value: 2020-12-31
attributes.tag: External Id
attributes.value: SAMEA7757826
attributes.tag: INSDC center alias
attributes.value: NEWCASTLE UNIVERSITY
attributes.tag: INSDC center name
attributes.value: NEWCASTLE UNIVERSITY
attributes.tag: INSDC first public
attributes.value: 2021-03-01T12:41:21Z
attributes.tag: INSDC last update
attributes.value: 2020-12-31T15:21:41Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: 33
attributes.tag: amount or size of sample collected
attributes.value: 0.5
attributes.tag: collection date
attributes.value: 2017-11-01
attributes.tag: environment (biome)
attributes.value: small river biome
attributes.tag: environment (feature)
attributes.value: river
attributes.tag: environment (material)
attributes.value: river water
attributes.tag: geographic location (country and/or sea)
attributes.value: Malaysia
attributes.tag: geographic location (depth)
attributes.value: 0.05
attributes.tag: geographic location (latitude)
attributes.value: 1.54
attributes.tag: geographic location (longitude)
attributes.value: 103.66
attributes.tag: geographic location (region and locality)
attributes.value: Southern Peninsular Malaysia, Johor, Skudai catchment
attributes.tag: investigation type
attributes.value: metagenome
attributes.tag: nucleic acid amplification
attributes.value: SOP Schloss MiSeq Wet Lab
attributes.tag: nucleic acid extraction
attributes.value: FastDNA SPIN kit for soil (MP Biomedicals)
attributes.tag: project name
attributes.value: Skudai catchment project
attributes.tag: sample collection device or method
attributes.value: Surface water collection with a bucket from middle of bridges
attributes.tag: sample material processing
attributes.value: Water collection, storage on ice until arrival in lab within 6 hours, then filtration 500 mL onto 0.22 um cellulose-nitrate-filters and storage of the filters in Eppendorf tube at -20oC until extraction
attributes.tag: sample name
attributes.value: 33
attributes.tag: sequencing method
attributes.value: Illumina MiSeq V2 500 cycles
attributes.tag: target gene
attributes.value: 16S
attributes.tag: target subfragment
attributes.value: V4 515F - 806R
attributes.tag: water environmental package
attributes.value: water