description: Protocols: Samples were placed on ice, rinsed with PBS and lysed in Trizol. Hind limbs were dissected and bone marrow macrophages were collected from female 6 to 8-week-old Control, Phlpp1 cKOCtsk or Phlpp1-/- mice as previously described (16). Briefly, cells were flushed, pelleted and red blood cells were lysed (RBC Lysis Buffer, #00-4333-57, Invitrogen, Carlsbad, CA). Cells were pelleted and cultured overnight in phenol red-free alpha MEM, 10% FBS and 1% antibiotic/antimycotic supplemented with 35 ng/mL M-CSF (#416-ML, R&D, Minneapolis, MN). Non-adherent cells were then placed in culture medium supplemented with 35 ng/mL M-CSF, and 90 ng/mL RANKL (#315-11, Prepro Tech, Rocky Hill, NJ). Cultures were fed on day 3 with culture medium plus 35 ng/ml M-CSF, and 90 ng/mL RANKL Trizol extraction Random hexamers were used to generate cDNA libraries via reverse transcription
attributes.tag: ENA first public
attributes.value: 2021-05-26
attributes.tag: ENA last update
attributes.value: 2021-05-26
attributes.tag: External Id
attributes.value: SAMEA8784586
attributes.tag: INSDC center alias
attributes.value: UNIVERSITY OF MINNESOTA
attributes.tag: INSDC center name
attributes.value: UNIVERSITY OF MINNESOTA
attributes.tag: INSDC first public
attributes.value: 2021-05-26T04:19:51Z
attributes.tag: INSDC last update
attributes.value: 2021-05-26T04:19:51Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-10487:WT
attributes.tag: age
attributes.value: 6 to 8
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: osteoclast progenitor cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: bone marrow
attributes.tag: progenitor cell type
attributes.value: macrophage
attributes.tag: sample name
attributes.value: E-MTAB-10487:WT
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: C57BL/6