description: 1 mL of a ML5 transposon library aliquot grown in LB+ 50µg/mL kanamycin to an OD600 of ~1.0  and saved at -80 ˚C was inoculated into 25 mL M9 + 20 mM D-glucose (125 mL baffled flask) and grown to an OD600 of ~1.0 (shaking 225 RPM) prior to pelleting and storing at -80 ˚C
attributes.tag: strain
attributes.value: KT2440 (ML5)
attributes.tag: isolate
attributes.value: 1C
attributes.tag: isolation_source
attributes.value: Tc1 mutagenesis of KT2440
attributes.tag: collection_date
attributes.value: 2021-12-07
attributes.tag: geo_loc_name
attributes.value: USA
attributes.tag: sample_type
attributes.value: Cell culture
attributes.tag: biomaterial_provider
attributes.value: Adam M Deutschbauer, Lawrence Berkeley National Laboratory
attributes.tag: collected_by
attributes.value: Andrew Borchert, Alissa Bleem, and William Cordell
attributes.tag: genotype
attributes.value: Tc1 insertion library of KT2440 (pWW0- and restriction- derivative of P. putida mt-2 )
attributes.tag: samp_size
attributes.value: 1 mL
attributes.tag: temp
attributes.value: 30 ˚C
attributes.tag: BioSampleModel
attributes.value: Microbe, viral or environmental