attributes.tag: isolate
attributes.value: not collected
attributes.tag: age
attributes.value: not collected
attributes.tag: biomaterial_provider
attributes.value: IOB, Apleona HSG AG, Building K-26, Klybeckstrasse 141, 4057 Basel, Switzerland
attributes.tag: sex
attributes.value: not collected
attributes.tag: tissue
attributes.value: CD45+ PBMC
attributes.tag: cell_type
attributes.value: PBMC
attributes.tag: plate
attributes.value: 210
attributes.tag: well
attributes.value: H4
attributes.tag: run_seq
attributes.value: 210122_NB551561_0039_AHH5VCBGXH
attributes.tag: cell
attributes.value: PBMC
attributes.tag: method
attributes.value: FLASH-Seq
attributes.tag: lysis buffer
attributes.value: TRITON_LOW
attributes.tag: lysis reaction volume (uL)
attributes.value: 5
attributes.tag: denaturation temperature (Celsius)
attributes.value: 72
attributes.tag: denaturation time (min)
attributes.value: 3
attributes.tag: oligo-dT dilution factor
attributes.value: 5
attributes.tag: RT enzyme
attributes.value: SIV
attributes.tag: RT temperature (Celsius)
attributes.value: 41.7
attributes.tag: RT duration (min)
attributes.value: 30
attributes.tag: TSO (x)
attributes.value: 1
attributes.tag: PCR duration (minutes)
attributes.value: 4
attributes.tag: PCR cycles
attributes.value: 21
attributes.tag: Forward / Reverse primer ratio
attributes.value: NA
attributes.tag: dNTP (x)
attributes.value: 1
attributes.tag: dCTP (mM)
attributes.value: 3.3
attributes.tag: ET Tth (ng)
attributes.value: 0
attributes.tag: T4g32p (uL)
attributes.value: 0.5
attributes.tag: NaCl (mM)
attributes.value: 0
attributes.tag: Sequencing
attributes.value: 75_SINGLE_END
attributes.tag: Library preparation method
attributes.value: NEXTERAXT
attributes.tag: Tn5 amount (uL)
attributes.value: NA
attributes.tag: Library cDNA input (ng)
attributes.value: NA
attributes.tag: BioSampleModel
attributes.value: Human