description: RNA was extracted from ten different tissues (2-week-old seedlings; 6-week-old plant root, stem and leaf; flowers; seed; 10-week-old senescent leaf; leaves from 4 week-old plants 1 hour after wounding; and leaves of 4 week-old plants 24 and 48-hours after Botrytis cinerea infection) according to standard CTAB protocols followed by lithium chloride precipitation. Samples were pooled and first-strand cDNA synthesized according to Pacific Biosciences (Menlo Park, USA) Isoseq recommended protocol. Library preparation and sequencing were performed according to Pacific Biosciences protocols by the UC Davis Genome Center using PacBio Single Molecule, Real-Time (SMRT) sequencing technology.
attributes.tag: cultivar
attributes.value: Arusha
attributes.tag: dev_stage
attributes.value: pooled
attributes.tag: geo_loc_name
attributes.value: United Kingdom
attributes.tag: tissue
attributes.value: pooled
attributes.tag: BioSampleModel
attributes.value: Plant