description: Protocols: All primary human breast tissue was derived from women undergoing reduction mammoplasties with no known genetic history (n = 4) and prophylactic mastectomies from women with germline BRCA1 mutations (n = 4) obtained from the Breast Cancer Now Tissue bank (15/EE/0192) Sorted cells were spun down and resuspended in RLT, and RNA was extracted using the RNeasy micro kit (Qiagen) according to manufacturer instructions. DNA was degraded by adding 20U Rnase-free DnaseI (Roche) for 30 min at room temperature. DnaseI treatment was performed on columns.   RNA samples were QC'd with Qubit and Tapestation. 3 ug of total RNA was next used in the NEBNext® Poly(A) mRNA Magnetic Isolation Module (E7490). Next, 30 ng of the PolyA RNA was used with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760), with 8 cycles of PCR. QC of the finished libraries was done with Qubit and Tapestation.
attributes.tag: ENA first public
attributes.value: 2021-02-02
attributes.tag: ENA last update
attributes.value: 2021-01-26
attributes.tag: External Id
attributes.value: SAMEA7874050
attributes.tag: INSDC center alias
attributes.value: UNIVERSITY OF CAMBRIDGE
attributes.tag: INSDC center name
attributes.value: UNIVERSITY OF CAMBRIDGE
attributes.tag: INSDC first public
attributes.value: 2021-02-02T17:08:01Z
attributes.tag: INSDC last update
attributes.value: 2021-01-26T22:36:38Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-10046:2987PM
attributes.tag: age
attributes.value: 26
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: clinical history
attributes.value: prophylactic mastectomy
attributes.tag: clinical information
attributes.value: germline BRCA1 mutation
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: individual
attributes.value: 2987PM
attributes.tag: organism part
attributes.value: breast
attributes.tag: sample name
attributes.value: E-MTAB-10046:2987PM
attributes.tag: sex
attributes.value: female