description: Protocols: Cell lines CT-26, 4T1 and MC-38 we purchased from ATCC. CT-26 (5x10^6 cells/mouse) or MC-38 (5x10^6 cells/mouse) tumor cells were implanted subcutaneously (s.c.) in the flank of female Balb/c and C57/Bl6 mice, respectively. 4T1 (5x10^6 cells/mouse) tumor cells were implanted orthotopically in mammary fat pad (o.t.) of female Balb/c mice or sub-cutaneous. At end of study tumor tissues were then transferred into the gentleMACS C Tube containing RPMI. Cells were liberated from tumors for downstream application using a mouse tumor dissociation kit from Miltenyi Biotec. and octodissociator (Miltenyi) according to manufacturer's instructions. For RNA sequencing, total RNA was extracted using the RNeasy 96 Qiacube HT Kit (Qiagen), quality validated using nanodrop and Quantit RNA Assay Kit (Thermo Fisher). For Tumour and Lymph-Node libraries were made using TruSeq stranded mRNA kit and sequenced on the NovaSeq system (100bp, single end). For peripheral blood, libraries were made using TruSeq total RNA stranded with globin depletion. For sorted macrophages, libraries were made using clontech ultra low input mRNA kit.
attributes.tag: ENA first public
attributes.value: 2021-02-07
attributes.tag: ENA last update
attributes.value: 2021-01-28
attributes.tag: External Id
attributes.value: SAMEA7887313
attributes.tag: INSDC center alias
attributes.value: AstraZeneca
attributes.tag: INSDC center name
attributes.value: AstraZeneca
attributes.tag: INSDC first public
attributes.value: 2021-02-07T04:07:31Z
attributes.tag: INSDC last update
attributes.value: 2021-01-28T13:21:24Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-10057:PI3Kgo1813_Tum_21
attributes.tag: allograft
attributes.value: mice were injected with tumorigenic MC38 cells
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: tumour
attributes.tag: sample name
attributes.value: E-MTAB-10057:PI3Kgo1813_Tum_21
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: BALB/c