description: Protocols: Flp-In 293 T-REx (Thermo Fisher Scientific) WT, UPF3B-KO and UPF3A-UPF3B dKO cells were maintained at 37° C, 5% CO2 and 90% humidity in Dublecco's Modified Eagle Medium (DMEM, Thermo Fisher Scientific) supplemented with 9% fetal bovine serum (FBS) and Penicillin-Streptomycin (both Thermo Fisher Scientific). For siRNA transfection, cells were reverse transfected with 60 pmol siRNA targeting UPF3A (or Luc in the negative control) per 2 x 10^5 cells using 2.5 μl Lipofectamine RNAiMAX. Total RNA was extracted using the Direct-zol RNA MiniPrep kit including the recommended DNase I treatment (Zymo Research) according to the manufacturer's instructions. Ribosomal depletion and strand specific library preparation was carried out with TruSeq Stranded Total RNA (with Ribo-Zero Gold) according to the manufacturer's instructions.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-04-26
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-04-26
attributes.tag: External Id
attributes.value: SAMEA9389628
attributes.tag: INSDC center alias
attributes.value: Gehring Lab Institute for Genetics University of Cologne
attributes.tag: INSDC center name
attributes.value: Gehring Lab Institute for Genetics University of Cologne
attributes.tag: INSDC first public
attributes.value: 2022-04-26T16:19:05Z
attributes.tag: INSDC last update
attributes.value: 2022-04-26T16:19:05Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-10716:A006200071_119403
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: Flp-In-T-REx-293
attributes.tag: cell_type
attributes.value: epithelial cell
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: late embryonic stage
attributes.tag: disease
attributes.value: normal
attributes.tag: genetic modification
attributes.value: none
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: kidney
attributes.tag: replicate
attributes.value: 2
attributes.tag: sample name
attributes.value: E-MTAB-10716:A006200071_119403