description: Protocols: Flp-In 293 T-REx (Thermo Fisher Scientific) WT and UPF3B-KO cells were maintained at 37° C, 5% CO2 and 90% humidity in Dublecco's Modified Eagle Medium (DMEM, Thermo Fisher Scientific) supplemented with 9% fetal bovine serum (FBS) and Penicillin-Streptomycin (both Thermo Fisher Scientific). For siRNA transfection, cells were reverse transfected with 60 pmol siRNA targeting UPF3A (or Luc in the negative control) per 3 x 10^5 cells using 2.5 μl Lipofectamine RNAiMAX. Total RNA was extracted using peqGOLD TriFast (VWR Peqlab) according to the manufacturer's instructions. Ribosomal depletion and strand specific library preparation was carried out with TruSeq Stranded Total RNA (with Ribo-Zero Gold) according to the manufacturer's instructions.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-04-26
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-04-26
attributes.tag: External Id
attributes.value: SAMEA9372237
attributes.tag: INSDC center alias
attributes.value: Gehring Lab Institute for Genetics University of Cologne
attributes.tag: INSDC center name
attributes.value: Gehring Lab Institute for Genetics University of Cologne
attributes.tag: INSDC first public
attributes.value: 2022-04-26T16:19:05Z
attributes.tag: INSDC last update
attributes.value: 2022-04-26T16:19:05Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-10711:K002000269_94806
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: Flp-In-T-REx-293
attributes.tag: cell_type
attributes.value: epithelial cell
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: late embryonic stage
attributes.tag: disease
attributes.value: normal
attributes.tag: genetic modification
attributes.value: none
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: kidney
attributes.tag: replicate
attributes.value: 3
attributes.tag: sample name
attributes.value: E-MTAB-10711:K002000269_94806