description: Protocols: HeLa Flp-In-T-REx (established by Elena Dobrikova and Matthias Gromeier, Duke University Medical Center) WT and UPF3A overexpressing cells were maintained at 37° C, 5% CO2 and 90% humidity in Dublecco's Modified Eagle Medium (DMEM, Thermo Fisher Scientific) supplemented with 9% fetal bovine serum (FBS) and Penicillin-Streptomycin (both Thermo Fisher Scientific). To induce expression of the stably integrated UPF3A construct, 30 μg/ml cumate was added and the cells were harvested after 48h. Total RNA was extracted using the Direct-zol RNA MiniPrep kit including the recommended DNase I treatment (Zymo Research) according to the manufacturer's instructions. Ribosomal depletion and strand specific library preparation was carried out with TruSeq Stranded Total RNA (with Ribo-Zero Gold) according to the manufacturer's instructions.
attributes.tag: ENA first public
attributes.value: 2022-04-26
attributes.tag: ENA last update
attributes.value: 2022-04-26
attributes.tag: External Id
attributes.value: SAMEA11299158
attributes.tag: INSDC center alias
attributes.value: Gehring Lab Institute for Genetics University of Cologne
attributes.tag: INSDC center name
attributes.value: Gehring Lab Institute for Genetics University of Cologne
attributes.tag: INSDC first public
attributes.value: 2022-04-26T16:19:20Z
attributes.tag: INSDC last update
attributes.value: 2022-04-26T16:19:20Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11184:156268
attributes.tag: age
attributes.value: 31
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: HeLa Flp-In-T-REx
attributes.tag: cell type
attributes.value: epithelial cell
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: cervical adenocarcinoma
attributes.tag: ethnic group
attributes.value: African American
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: uterine cervix
attributes.tag: protocol
attributes.value: ERCC spike in
attributes.tag: replicate
attributes.value: 1
attributes.tag: sample name
attributes.value: E-MTAB-11184:156268
attributes.tag: sex
attributes.value: female