description: Protocols: Individual organoids were flash frozen in isopentane on dry ice at day 14 and stored at -80. Samples were thawed in 'RNAlater-ICE (Thermo Fisher) at -20 overnight prior to RNA extraction. Patterning factors were added in low and high concentrations from day 3 to day 6 of organoid development. RNA extraction with Trizol and 1-Bromo-3-chloropropane, bead based RNA purification (Thermo Fisher's MagMax kit - spin procedure) Bulk RNA-seq libraries were constructed using the Smartseq2 protocol. Individual samples were indexed with Nextera XT Index kit v2, set B.
attributes.tag: ENA first public
attributes.value: 2021-02-11
attributes.tag: ENA last update
attributes.value: 2021-02-01
attributes.tag: External Id
attributes.value: SAMEA7965672
attributes.tag: INSDC center alias
attributes.value: ETH Zurich, DBSSE
attributes.tag: INSDC center name
attributes.value: ETH Zurich, DBSSE
attributes.tag: INSDC first public
attributes.value: 2021-02-11T04:10:17Z
attributes.tag: INSDC last update
attributes.value: 2021-02-01T17:08:25Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-10037:PS2_C6_RSPO2high
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: HES-3, NKX2.1 (GFP/w)
attributes.tag: cell type
attributes.value: embryonic stem cell
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: blastula
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: NKX2.1-GFP/+
attributes.tag: karyotype
attributes.value: 46XX
attributes.tag: organism part
attributes.value: inner cell mass
attributes.tag: sample name
attributes.value: E-MTAB-10037:PS2_C6_RSPO2high