description: Protocols: Trunks containing the lesion sites (or equivalent site from unlesioned fish) are collected and kept in PBS on ice.Incubate the trunks (up to 300) in 1 mL of 1X Trypsin-EDTA at 37C for 5-7 mins.Stop dissociation by adding FBS to a final concentration of 5%.Centrifuge at 200g for 7 mins.Discard Sups.Resuspend in 500 uL of PBS.Add the cell suspension into a 40 uM cell strainer.Centrifuge at 200g for 7 mins.Resuspend in the buffer for FACS (PBS 5% FBS) Following cell dissociation and FAC sorting, samples were processed on the 10X Chromium platform using 10X Single Cell 3' v3 chemistry following the manufacturer's guidelines Following cell dissociation and FAC sorting, samples were processed on the 10X Chromium platform using 10X Single Cell 3' v3 chemistry following the manufacturer's guidelines
attributes.tag: ENA first public
attributes.value: 2021-07-08
attributes.tag: ENA last update
attributes.value: 2021-07-08
attributes.tag: External Id
attributes.value: SAMEA9361876
attributes.tag: INSDC center alias
attributes.value: UOE
attributes.tag: INSDC center name
attributes.value: University Of Edinburgh
attributes.tag: INSDC first public
attributes.value: 2021-07-08T11:18:57Z
attributes.tag: INSDC last update
attributes.value: 2021-07-08T11:18:57Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-10379_3:Naive
attributes.tag: age
attributes.value: 4
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: mixed macrophage and microglia
attributes.tag: common name
attributes.value: zebrafish
attributes.tag: developmental stage
attributes.value: larval day 4
attributes.tag: immunophenotype
attributes.value: mpeg1.1 positive
attributes.tag: organism part
attributes.value: spinal cord
attributes.tag: sample name
attributes.value: E-MTAB-10379_3:Naive
attributes.tag: sex
attributes.value: mixed
attributes.tag: strain
attributes.value: WIK