description: Protocols: Surgical specimens were collected and either (i) snap-frozen and stored at -70oC or (ii) fixed in formalin and embedded in paraffin. For fresh frozen tissues, RNA and DNA were isolated using QIAGEN RNAeasy and DNAeasy kits, respectively, according to the manufacturer's protocols. For FFPE tissues, RNA and DNA were isolated with QIAGEN AllPrep FFPE Kit, according to the manufacturer's protocols. DNA was prepared for sequencing using the Agilent Technologies SureSelect XT library protocol. Fresh-frozen tumors were processed according to the manufacturer's protocol for 3 μg input, while FFPE tumors were processed with the low-volume input according to the manufacturer's protocol for 200 ng input. DNA libraries were captured and amplified with Agilent Technologies SureSelect Human All Exon, version 5 for fresh frozen tissues or version 6 for FFPE tissues, according to the manufacturer's protocol. The quality of both the DNA libraries and DNA exome capture and concentration were quantified with Agilent TapeStation DNA 1000 and High Sensitivity D1000, respectively.
attributes.tag: ENA first public
attributes.value: 2021-02-02
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-06-22
attributes.tag: External Id
attributes.value: SAMEA7965867
attributes.tag: INSDC center alias
attributes.value: UOE
attributes.tag: INSDC center name
attributes.value: University Of Edinburgh
attributes.tag: INSDC first public
attributes.value: 2021-02-02T17:08:01Z
attributes.tag: INSDC last update
attributes.value: 2022-06-22T10:42:08Z
attributes.tag: INSDC status
attributes.value: suppressed
attributes.tag: Submitter Id
attributes.value: E-MTAB-10080:MDR7-Normal-2C
attributes.tag: age
attributes.value: 87
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: estrogen-receptor positive breast cancer
attributes.tag: individual
attributes.value: MDR7
attributes.tag: organism part
attributes.value: breast
attributes.tag: sample name
attributes.value: E-MTAB-10080:MDR7-Normal-2C
attributes.tag: sampling site
attributes.value: normal tissue adjacent to neoplasm
attributes.tag: sex
attributes.value: female
attributes.tag: specimen with known storage state
attributes.value: Node FFPE