description: Protocols: 6 dpf zebrafish larvae were anesthetized and decapitated using a razor blade. 10 heads were pooled per sample. Total RNA was extracted using TRIzol reagent (Invitrogen), followed by phenol-chlorophorm extraction and isopropanol precipitation. After ethanol washes, the RNA pellet was air-dried, dissolved in nuclease-free water (Fermentas). TruSeq RNA-Seq sample preparation kit was used to prepare sequencing library of mRNA in accordance with the manufacturer guidelines.
attributes.tag: ENA first public
attributes.value: 2022-04-21
attributes.tag: ENA last update
attributes.value: 2022-04-21
attributes.tag: External Id
attributes.value: SAMEA13254180
attributes.tag: INSDC center alias
attributes.value: Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg
attributes.tag: INSDC center name
attributes.value: Laboratory for Molecular Biodiscovery, KU Leuven, Campus Gasthuisberg
attributes.tag: INSDC first public
attributes.value: 2022-04-21T00:32:07Z
attributes.tag: INSDC last update
attributes.value: 2022-04-21T00:32:07Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11505:Sv2aWT4S7
attributes.tag: age
attributes.value: 6
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: zebrafish
attributes.tag: developmental stage
attributes.value: larval stage
attributes.tag: genotype
attributes.value: sv2a+/+
attributes.tag: individual
attributes.value: pool of 10 heads
attributes.tag: organism part
attributes.value: head
attributes.tag: sample name
attributes.value: E-MTAB-11505:Sv2aWT4S7
attributes.tag: sex
attributes.value: not available
attributes.tag: strain
attributes.value: AB