description: Protocols: The conditional B6.129S/Sv-Trp53<tm1Thst> (Trp53LSL-E170R) knock-in mouse was described earlier (Timofeev et al., 2013). Homozygous Trp53LSL-E170R/LSL-E170R mice with the intact LSL cassette (deficient for p53 expression) were used as p53-null controls. RNA was isolated using the RNeasy Mini kit (Qiagen) and RNA quality was assessed using the Experion RNA StdSens Analysis Kit (Bio‐Rad). RNA-seq libraries were prepared using the TruSeq Stranded mRNA Library Prep kit (Illumina).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-04-26
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-04-26
attributes.tag: External Id
attributes.value: SAMEA8251209
attributes.tag: INSDC center alias
attributes.value: Institute of Molecular Oncology, Philipps-University Marburg
attributes.tag: INSDC center name
attributes.value: Institute of Molecular Oncology, Philipps-University Marburg
attributes.tag: INSDC first public
attributes.value: 2022-04-26T16:15:53Z
attributes.tag: INSDC last update
attributes.value: 2022-04-26T16:15:53Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-10216:p53-E177R-9
attributes.tag: age
attributes.value: 16 to 20
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: acute myeloid leukemia
attributes.tag: genotype
attributes.value: p53 E177R/E177R
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: blood
attributes.tag: sample name
attributes.value: E-MTAB-10216:p53-E177R-9
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: C57BL/6J x 129S