description: Protocols: Samples were collected by the Human Developmental Biology Resource and subsequently dissected by their team. Single cells suspensions were made by mechanical digestion and live, enriched cells were selected by FACS. Nucleic acid extraction was conducted using an integrated bead-based approach, with cell lysis being a component of the sequencing A slightly modified Smart-seq2 protocol was used. After cDNA generation, libraries were prepared (384 cells per library) using the Illumina Nextera XT kit (Illumina). Index v.2 sets A, B, C and D were used per library to barcode each cell before multiplexing.
attributes.tag: ENA first public
attributes.value: 2021-07-08
attributes.tag: ENA last update
attributes.value: 2021-07-08
attributes.tag: External Id
attributes.value: SAMEA7618581
attributes.tag: INSDC center alias
attributes.value: Haniffa Lab
attributes.tag: INSDC center name
attributes.value: Haniffa Lab
attributes.tag: INSDC first public
attributes.value: 2021-07-08T00:11:40Z
attributes.tag: INSDC last update
attributes.value: 2021-07-08T00:11:40Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-9801:151-F90-P2-Mast-19-Muzz-Haniffa_S141
attributes.tag: age
attributes.value: 17
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: embryo stage
attributes.tag: disease
attributes.value: normal
attributes.tag: individual
attributes.value: F90
attributes.tag: inferred cell type
attributes.value: eosinophil
attributes.tag: organism part
attributes.value: bone marrow
attributes.tag: sample name
attributes.value: E-MTAB-9801:151-F90-P2-Mast-19-Muzz-Haniffa_S141
attributes.tag: sex
attributes.value: female
attributes.tag: well identifier
attributes.value: P2_E7