description: Protocols: Cells were dissociated in Accutase for 5 min and pipetted vigorously to obtain a single cell suspension in normal culture media. Single cells were picked by mouth pipette using a blunted microcapillary with internal diameter 15 μM and under a dissection microscope. Cells were collected into 0.2 mL tubes with 4 μL lysis buffer and immediately snap frozen on dry ice. For each sample, RNA separation was performed using biotinylated oligo-dT30VN-tailed oligonucleotides (IDT) conjugated to Dynabeads Streptavidin C1 (65001, Invitrogen) in an RNAse-inhibitor (RNAsin; N2615, Promega) supplemented buffer solution. Sequencing libraries were prepared according to an adapted version of the G&T protocol previously described. Samples were transferred to a 96-well plate and prepared for both transcriptome and methylome sequencing, by physical separation of genomic DNA (gDNA) from messenger RNA (mRNA).
attributes.tag: ENA first public
attributes.value: 2021-07-08
attributes.tag: ENA last update
attributes.value: 2021-07-08
attributes.tag: External Id
attributes.value: SAMEA7075799
attributes.tag: INSDC center alias
attributes.value: UNIVERSITY OF CAMBRIDGE
attributes.tag: INSDC center name
attributes.value: UNIVERSITY OF CAMBRIDGE
attributes.tag: INSDC first public
attributes.value: 2021-07-08T00:11:25Z
attributes.tag: INSDC last update
attributes.value: 2021-07-08T00:11:25Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-9367:SLX_16564.i710_i508.HWCLLBBXX.s_8.r
attributes.tag: age
attributes.value: 25
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: amion
attributes.tag: common name
attributes.value: white-tufted-ear marmoset
attributes.tag: developmental stage
attributes.value: Carnegie stage 7
attributes.tag: individual
attributes.value: 7
attributes.tag: organism part
attributes.value: amnion
attributes.tag: post analysis well quality
attributes.value: pass
attributes.tag: sample name
attributes.value: E-MTAB-9367:SLX_16564.i710_i508.HWCLLBBXX.s_8.r
attributes.tag: single cell well quality
attributes.value: OK