description: Reverse genetics using CRISPR-Cas9 in the tropical tree Parasponia andersonii revealed a promotive role for PanNODULE ROOT1 in stem secondary growth
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2021-05-03T00:24:49Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2020-04-14T15:32:09Z
attributes.tag: External Id
attributes.value: SAMEA6675633
attributes.tag: INSDC center name
attributes.value: WAGENINGEN UNIVERSITY (Bioinformatics)
attributes.tag: INSDC first public
attributes.value: 2021-05-03T00:24:49Z
attributes.tag: INSDC last update
attributes.value: 2020-04-14T15:32:09Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: parasponia_andersonii26
attributes.tag: air temperature regimen
attributes.value: 28degrees
attributes.tag: collection date
attributes.value: 2019
attributes.tag: culture rooting medium
attributes.value: SH salts 3.2 g.L-1, and SH vitamins 1 g.L-1
attributes.tag: estimated size
attributes.value: 550mb
attributes.tag: genetic modification
attributes.value: Empty vector transformation
attributes.tag: genotype
attributes.value: Ctr44
attributes.tag: geographic location (country and/or sea)
attributes.value: Netherlands
attributes.tag: geographic location (latitude)
attributes.value: 51.9669161323
attributes.tag: geographic location (longitude)
attributes.value: 5.65515904602
attributes.tag: growth facility
attributes.value: growth chamber
attributes.tag: light regimen
attributes.value: 16/8h day/night
attributes.tag: mineral nutrient regimen
attributes.value: nutritive solution (NH4, 1 mmol.L-1; K, 5.9 mmol.L-1; Ca; 2.7 mmol.L-1; Mg, 0.8 mmol.L-1; NO3, 9 mmol.L-1; SO4, 1.9 mmol.L-1; P, 1.1 mmol.L-1; Fe, 15 µmol.L-1; Mn, 5 µmol.L-1; Zn, 5 µmol.L-1; B, 3 µmol.L-1; Cu, 0.5 µmol.L-1; Mo, 0.5 µmol.L-1; pH: 5.8)
attributes.tag: plant developmental stage
attributes.value: stem internode 3
attributes.tag: plant growth medium
attributes.value: Schenk & Hildebrandt (SH)
attributes.tag: plant structure
attributes.value: stem
attributes.tag: ploidy
attributes.value: 2n
attributes.tag: rooting medium carbon
attributes.value: sucrose 10 g.L-1
attributes.tag: rooting medium pH
attributes.value: 5.8
attributes.tag: rooting medium regulators
attributes.value: IBA 1 µg.mL-1; NAA 0.1 µg.mL-1; MES 3 mM
attributes.tag: rooting medium solidifier
attributes.value: Daishin agar
attributes.tag: sample health state
attributes.value: healthy
attributes.tag: sample name
attributes.value: parasponia_andersonii26
attributes.tag: scientific_name
attributes.value: Parasponia andersonii
attributes.tag: watering regimen
attributes.value: 2times/week water or nutritive solution