description: Protocols: 1x106 Fh1 fl/fl or 2x106 Fh1 -/- cells were plated onto 15-cm dishes (Nunc) and allowed to reach 80% confluency. Cells were then washed twice in PBS and detached using trypsin (Gibco, 0.25% in PBS-EDTA). After inactivating trypsin with complete medium, cells were filtered through 70μm cell strainers (Fisher Scientific) and counted with Cell Counter CASY (OmniLife Sciences). Following an additional wash in PBS, 7x106 cells were resuspended in 500 μl of PBS and crosslinked using formaldehyde (Thermo Fisher) at 1% final concentration. Crosslinking was stopped using glycine solution after 10 min and 3 washes were carried in cold PBS before storing the pellets at -80˚C. Cells were processed using iDeal ChIP-seq kit for Histones (Diagenode, C01010051) DNA Library was generated using NEB Next Ultra II DNA library kit (NEB, E7645S) following the manufacturer's protocol
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-06-17
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-06-17
attributes.tag: External Id
attributes.value: SAMEA110001512
attributes.tag: INSDC center alias
attributes.value: MRC Cancer Unit
attributes.tag: INSDC center name
attributes.value: MRC Cancer Unit
attributes.tag: INSDC first public
attributes.value: 2022-06-17T00:17:34Z
attributes.tag: INSDC last update
attributes.value: 2022-06-17T00:17:34Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11790:pFH H3K27ac 3
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: Fh1 -/- +pFH
attributes.tag: cell_type
attributes.value: epithelial cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: hereditary leiomyomatosis and renal cell cancer
attributes.tag: genotype
attributes.value: Fh1 knockout
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: kidney
attributes.tag: replicate
attributes.value: 3
attributes.tag: sample name
attributes.value: E-MTAB-11790:pFH H3K27ac 3