description: Protocols: Following a 75 minutes shift at 37°C, after reaching exponential phase at 25°C in YPD, cells were cross-linked with 1% formaldehyde during 10 minutes. DNA was extracted using the Auto iPure V2 kit from Diagenode, following the manufacturer recommendations. Library construction was done according to the takara SMARTER thruplex (takara bio) protocol, following manufacturer's recommendation. Libraries were sized with AMPure beads (Beckman-coulter) to a final size of 325bp.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-06-17
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-06-17
attributes.tag: External Id
attributes.value: SAMEA9637902
attributes.tag: INSDC center alias
attributes.value: Universite Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), 91198 Gif-sur-Yvette cedex, France
attributes.tag: INSDC center name
attributes.value: Universite Paris-Saclay, CEA, CNRS, Institute for Integrative Biology of the Cell (I2BC), 91198 Gif-sur-Yvette cedex, France
attributes.tag: INSDC first public
attributes.value: 2022-06-17T12:16:22Z
attributes.tag: INSDC last update
attributes.value: 2022-06-17T12:16:22Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-10828:Rad10_kin28ts
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: developmental stage
attributes.value: exponential phase
attributes.tag: genotype
attributes.value: MATα ade2-1 lys2-801uag ura3-52 trp1-∆63 his3-∆200 leu2-∆ RAD10::3HA::HIS3 MED17::13Myc::TRP1 kin28::KanMX6 CEN kin28-ts16 LEU2
attributes.tag: growth condition
attributes.value: YPD media
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: whole organism
attributes.tag: phenotype
attributes.value: temperature sensitive Kin28
attributes.tag: sample name
attributes.value: E-MTAB-10828:Rad10_kin28ts