description: Protocols: we deleted a 120-bp region at rs2280381 in the U-937 cell line using CRISPR/Cas9 with two sgRNAs targeted around rs2280381 loci. Plasmids expressing Cas9 and sgRNAs were nucleofected into cells, and cell clones were screened to obtain bi-allelic 120-bp deletion clones. Clones exposed to CRISPR/Cas9 complexes with wildtype genotype were selected as controls. Total RNA was extracted using TRIzol reagent (15596018, Thermo Fisher) 1 μg total RNA was purified to remove rRNA and was used to generate the cDNA libraries using Illumina NEBNext® Ultra™ Directional RNA Library Prep Kit
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-06-17
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-06-17
attributes.tag: External Id
attributes.value: SAMEA12280240
attributes.tag: INSDC center alias
attributes.value: Shanghai Institute of Rheumatology, Renji Hospital, Shanghai Jiao Tong University School of Medicine (SJTUSM), Shanghai0, China, 20001.
attributes.tag: INSDC center name
attributes.value: Shanghai Institute of Rheumatology, Renji Hospital, Shanghai Jiao Tong University School of Medicine (SJTUSM), Shanghai0, China, 20001.
attributes.tag: INSDC first public
attributes.value: 2022-06-17T12:16:48Z
attributes.tag: INSDC last update
attributes.value: 2022-06-17T12:16:48Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11306:WT3
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: U937
attributes.tag: cell_type
attributes.value: monocyte
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: lymphoma
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: sample name
attributes.value: E-MTAB-11306:WT3