title: slt2_5h_2
description: Protocols: 2X10(7)exponentially growing (5h post-inoculation) and early post-diauxic phase (13h post-inoculation) cells were harvested by centrifugation at 1000 x g for 5 min at 4°C. Total RNA was isolated from using an RNeasy Mini kit (Qiagen, 74104). After the QC procedures of total RNA, mRNA is enriched using oligo(dT) beads. Enriched mRNA is fragmented randomly by adding fragmentation buffer, then the cDNA is first synthesized by using random hexamers primer, after which a custom second-strand synthesis buffer (Illumina), dNTPs, RNase H and DNA polymerase I are added to initiate the second-strand synthesis. Second, after a series of terminal repair, A ligation and sequencing adaptor ligation, the double-stranded cDNA library is completed through size selection and PCR enrichment.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-09-01
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-09-01
attributes.tag: External Id
attributes.value: SAMEA10289521
attributes.tag: INSDC center alias
attributes.value: Department of Biochemistry University of Cambridge
attributes.tag: INSDC center name
attributes.value: Department of Biochemistry University of Cambridge
attributes.tag: INSDC first public
attributes.value: 2022-09-01T00:13:47Z
attributes.tag: INSDC last update
attributes.value: 2022-09-01T00:13:47Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11024:slt2_5h_2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: genotype
attributes.value: slt2 deletion
attributes.tag: growth condition
attributes.value: glucose-replete (exponential)
attributes.tag: growth stage
attributes.value: exponential phase
attributes.tag: organism part
attributes.value: whole organism
attributes.tag: replicate
attributes.value: 2
attributes.tag: sample name
attributes.value: E-MTAB-11024:slt2_5h_2
attributes.tag: strain
attributes.value: BY4742