title: Bulk soil sample Hv918
description: Bulk soil DNA sample from sample exposed to sufficient nitrogen supplies
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-09-01
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-09-01
attributes.tag: External Id
attributes.value: SAMEA110398723
attributes.tag: INSDC center alias
attributes.value: DUNDEE
attributes.tag: INSDC center name
attributes.value: University of Dundee
attributes.tag: INSDC first public
attributes.value: 2022-09-01T00:18:14Z
attributes.tag: INSDC last update
attributes.value: 2022-09-01T00:18:14Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: Hv918
attributes.tag: broad-scale environmental context
attributes.value: rural area [ENVO:01000772]
attributes.tag: collection date
attributes.value: 2015-02-16
attributes.tag: environmental medium
attributes.value: farm soil [ENVO:00005749]
attributes.tag: geographic location (country and/or sea)
attributes.value: United Kingdom
attributes.tag: geographic location (latitude)
attributes.value: 56.45139
attributes.tag: geographic location (longitude)
attributes.value: -3.074722
attributes.tag: host common name
attributes.value: Barley
attributes.tag: host taxid
attributes.value: 4513
attributes.tag: local environmental context
attributes.value: research station [ENVO:00003919]
attributes.tag: mineral nutrient regimen
attributes.value: Nutrient solutions were supplied by watering weekly at a rate of 25ml per Kg of soil. 100% N solution: 25mM 2(Nh4)SO4; 40mM Ca(NO3)2; 10mM KNO3; 3mM MgSO4; 100µM FeEDTA+; 1mM KH2PO4; 14 treatments were applied with a total of 312.5 mg (NO3-) and 81.4 mg (NH4+)
attributes.tag: pcr primers
attributes.value: 515F: GTGCCAGCMGCCGCGGTAA; 806R: GGACTACHVGGGTWTCTAAT
attributes.tag: project name
attributes.value: Barley NT
attributes.tag: sample name
attributes.value: Hv918
attributes.tag: sequencing method
attributes.value: MiSeq [OBI:0002003]
attributes.tag: source material identifiers
attributes.value: Hv918
attributes.tag: subspecific genetic lineage
attributes.value: Bulk
attributes.tag: target gene
attributes.value: 16S rRNA
attributes.tag: target subfragment
attributes.value: V4