title: aWWP.1
description: Protocols: Imaginal tissues were dissected in PBS 1x and incubated for 1h in a 10x trypsin solution (Sigma T4174) at room temperature in a rotating wheel. Cells were vigorously pipetted and kept on ice in Schneider's insect medium. To discard dead cells, DAPI was added to the sample at 1 μg/mL final concentration. Cells were sorted in a FACSAria (BD) with the 85 μm nozzle Imaginal tissues were dissected in PBS 1x Flies were grown in standard media at 25ºC. RNA from sorted cells was extracted with ZR-RNA MicroPrep Kit from Zymo Research following the manufacturer's instructions Sequencing libraries were prepared using TruSeq Stranded mRNA Library Preparation Kit from Illumina and following the manufacturer's instructions.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-09-01
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-09-01
attributes.tag: External Id
attributes.value: SAMEA9726766
attributes.tag: INSDC center alias
attributes.value: Centre for Genomic Regulation (CRG), The Barcelona Institute for Science and Technology (BIST), Barcelona , Catalonia, Spain.
attributes.tag: INSDC center name
attributes.value: Centre for Genomic Regulation (CRG), The Barcelona Institute for Science and Technology (BIST), Barcelona , Catalonia, Spain.
attributes.tag: INSDC first public
attributes.value: 2022-09-01T00:13:42Z
attributes.tag: INSDC last update
attributes.value: 2022-09-01T00:13:42Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-10879:aWWP.1
attributes.tag: age
attributes.value: 120
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: fruit fly
attributes.tag: developmental stage
attributes.value: pupal stage
attributes.tag: genotype
attributes.value: w-; en-GAL4:UAS-GFP/+
attributes.tag: immunophenotype
attributes.value: GFP-positive
attributes.tag: organism part
attributes.value: wing disc anterior compartment
attributes.tag: sample name
attributes.value: E-MTAB-10879:aWWP.1
attributes.tag: sex
attributes.value: mixed