title: Fh1 fl/fl FOXA2 rep2
description: Protocols: Cells were fixed with 1% HCHO for 10 minutes and scraped into ice-cold PBS. Cells were lysed with ChIP lysis buffers and chromatin was sonicated for 15 minutes. Chromatin was incubated with Dynabeads with either anti-Foxa2 or IgG antibody overnight at 4deg. Beads were washed and incubated with Tn5 buffer and Tn5 enzyme for 10 minutes at 37deg with vigorous shaking. DNA was then eluted from the beads and purified. Purified DNA was amplified using Nextera indexes.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-09-01
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-09-01
attributes.tag: External Id
attributes.value: SAMEA20004632
attributes.tag: INSDC center alias
attributes.value: MRC Cancer Unit
attributes.tag: INSDC center name
attributes.value: MRC Cancer Unit
attributes.tag: INSDC first public
attributes.value: 2022-09-01T00:14:16Z
attributes.tag: INSDC last update
attributes.value: 2022-09-01T00:14:16Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11785:Fh1 fl/fl FOXA2 rep2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: Fh1 fl/fl
attributes.tag: cell_type
attributes.value: epithelial cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: hereditary leiomyomatosis and renal cell cancer
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: kidney
attributes.tag: replicate
attributes.value: 2
attributes.tag: sample name
attributes.value: E-MTAB-11785:Fh1 fl/fl FOXA2 rep2