title: 032-4P-1D
description: Protocols: Surgical specimens were collected and either (i) snap-frozen and stored at -70oC or (ii) fixed in formalin and embedded in paraffin. For fresh frozen tissues, RNA and DNA were isolated using QIAGEN RNAeasy and DNAeasy kits, respectively, according to the manufacturer's protocols. For FFPE tissues, RNA and DNA were isolated with QIAGEN AllPrep FFPE Kit, according to the manufacturer's protocols. RNA quality was assessed with an Agilent BioAnalyzer RNA 6000 Nano Kit. Fresh-frozen RNA ribo-zero library was prepared with rRNA removed from total RNA using Epicentre's Ribo-Zero rRNA Removal kit (Cat# RZH11042). 30-100 ng Ribo-Zero RNA was used for the construction of the library using the Illumina TruSeq™ RNA Sample Prep Kit (Cat# RS-122-2001) and the manufacturer's instructions were followed, except for omitting the purification step before fragmentation. FFPE RNA was prepared with the Illumina TruSeq FFPE RiboZero Gold protocol according to the manufacturer's instructions.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-09-01
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-09-01
attributes.tag: External Id
attributes.value: SAMEA110364487
attributes.tag: INSDC center alias
attributes.value: UOE
attributes.tag: INSDC center name
attributes.value: University Of Edinburgh
attributes.tag: INSDC first public
attributes.value: 2022-09-01T00:14:21Z
attributes.tag: INSDC last update
attributes.value: 2022-09-01T00:14:21Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-9917_2:032-4P-1D
attributes.tag: age
attributes.value: 78
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: estrogen-receptor positive breast cancer
attributes.tag: individual
attributes.value: Patient 032
attributes.tag: organism part
attributes.value: mammary gland
attributes.tag: sample name
attributes.value: E-MTAB-9917_2:032-4P-1D
attributes.tag: sampling site
attributes.value: neoplasm
attributes.tag: sex
attributes.value: female
attributes.tag: specimen with known storage state
attributes.value: FFPE specimen