title: Sample 12
description: Protocols: Selection of transposon-insertion mutants in the presence of meropenem was performed in duplicate. Approximately 10^7 cells from the library were grown overnight at 37 °C in 1 mL of lysogeny broth containing meropenem at 2×, 1×, 0.5×, and 0.25× the MIC of 0.032 mg.mL−1. Control cultures without meropenem were grown in parallel. For each concentration of meropenem, cultures were grown in which 0 (uninduced), 0.2 or 1.0 mM IPTG was added. Genomic DNA was extracted using a Quick-DNA Fungal/Bacterial 96 kit (Zymo Research) according to the manufacturer's instructions. DNA was fragmented using a Nextera tagmentation kit (Illumina). The fragmented DNA was amplified by PCR using oligonucleotide primers that hybridised at the transposon ends and contained an Illumina adapter sequence at their 5′ end.
attributes.tag: ENA first public
attributes.value: 2022-06-09
attributes.tag: ENA last update
attributes.value: 2022-06-09
attributes.tag: External Id
attributes.value: SAMEA110055379
attributes.tag: INSDC center alias
attributes.value: Quadram Institute Bioscience
attributes.tag: INSDC center name
attributes.value: Quadram Institute Bioscience
attributes.tag: INSDC first public
attributes.value: 2022-06-09T00:14:55Z
attributes.tag: INSDC last update
attributes.value: 2022-06-09T00:14:55Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11802:Sample 12
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: replicate
attributes.value: 2
attributes.tag: sample name
attributes.value: E-MTAB-11802:Sample 12
attributes.tag: strain
attributes.value: BW25113