title: ATAC-seq_etoh
description: Protocols: ATAC-seq was performed with 100,000 cells per treatment, cells were grown in DMEM containing 5% fetal bovine serum. A549 cells where grown to approximately 90% confluence according to standard protocols in DMEM medium with 5% FBS. Cells were treated for 20 hours with 100 nM demxathasone, 100 nM hydrocortisone or 0.1% ethanol and either harvested immediately or washed 2x with PBS and subsequently cultured in hormone-free medium for 24 hours before harvest. ATAC-seq was performed with 100,000 cells per treatment according to the Omni-ATAC-seq protocol [PMID: 28846090]. DNA was extracted using the DNA Clean & Concentrator Kit (Zymo research #D4014). Library amplification involved 11 PCR cycles using the NEBNext High-Fidelity 2x PCR Master Mix (New England Labs, Cat #M0541).
attributes.tag: ENA first public
attributes.value: 2021-05-12
attributes.tag: ENA last update
attributes.value: 2021-05-12
attributes.tag: External Id
attributes.value: SAMEA7708396
attributes.tag: INSDC center name
attributes.value: MPIMG
attributes.tag: INSDC first public
attributes.value: 2021-05-03T00:22:16Z
attributes.tag: INSDC last update
attributes.value: 2020-12-14T15:35:54Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-9910:ATAC-seq_etoh
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: A549
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: carcinoma
attributes.tag: organism part
attributes.value: lung
attributes.tag: sample name
attributes.value: E-MTAB-9910:ATAC-seq_etoh