title: Sample 5
description: Protocols: Shoot tissues of plants were cut and flash frozen in liquid nitrogen. Seeds were first germinated in agar-solidified 0.5x MS medium for 2 weeks before transfer into soil (Minitray, Balster Einheitserdewerk, Fröndenberg, DE) and a growth chamber at 12-h light(120 µmol photons m-2 s-1, 22°C) and 12-h dark (18°C; CLF Plant Climatics, Emersacker, Germany). Total RNA was isolated by RNeasy Plant Mini Kit (Qiagen). mRNA was enriched using oligo(dT) bead. The mRNA is fragmented randomly by adding fragmentation buffer, then the cDNA is synthesized by using mRNA template and random hexamers primer, after which a custom second-strand synthesis buffer (Illumina) , dNTPs, RNase H and DNA polymerase I are added to initiate the second-strand synthesis. Second, after a series of terminal repair, A ligation and sequencing adaptor ligation, the double-stranded cDNA library is completed through size selection and PCR enrichment.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2021-12-28
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2021-12-28
attributes.tag: External Id
attributes.value: SAMEA11308518
attributes.tag: INSDC center alias
attributes.value: Molecular Genetics and Physiology of Plants, Ruhr University Bochum
attributes.tag: INSDC center name
attributes.value: Molecular Genetics and Physiology of Plants, Ruhr University Bochum
attributes.tag: INSDC first public
attributes.value: 2021-12-28T00:12:19Z
attributes.tag: INSDC last update
attributes.value: 2021-12-28T00:12:19Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11206:Sample 5
attributes.tag: age
attributes.value: 4
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: thale cress
attributes.tag: developmental stage
attributes.value: LP.16 sixteen leaves visible stage
attributes.tag: genotype
attributes.value: dph1[2]
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: shoot
attributes.tag: replicate
attributes.value: 2
attributes.tag: sample name
attributes.value: E-MTAB-11206:Sample 5
attributes.tag: ecotype
attributes.value: Col-0