title: ATAC-seq_etoh_washout
description: Protocols: ATAC-seq was performed with 100,000 cells per treatment, cells were grown in DMEM containing 5% fetal bovine serum. U2OS-GR cells where grown to approximately 90% confluence according to standard protocols in DMEM medium with 5% FBS. Cells were treated for 4 hours with 100 nM dexamethasone or 0.1% ethanol and either harvested immediately or washed 2x with PBS and subsequently cultured in hormone-free medium for 24 hours before harvest. ATAC-seq was performed with 100,000 cells per treatment according to the Omni-ATAC-seq protocol [PMID: 28846090]. DNA was extracted using the DNA Clean & Concentrator Kit (Zymo research #D4014). Library amplification involved 11 PCR cycles using the NEBNext High-Fidelity 2x PCR Master Mix (New England Labs, Cat #M0541).
attributes.tag: ENA first public
attributes.value: 2021-05-12
attributes.tag: ENA last update
attributes.value: 2021-05-12
attributes.tag: External Id
attributes.value: SAMEA7708529
attributes.tag: INSDC center name
attributes.value: MPIMG
attributes.tag: INSDC first public
attributes.value: 2021-05-03T00:22:16Z
attributes.tag: INSDC last update
attributes.value: 2020-12-14T16:06:41Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-9909:ATAC-seq_etoh_washout
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: U2OS-GR
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: osteosarcoma
attributes.tag: organism part
attributes.value: bone
attributes.tag: sample name
attributes.value: E-MTAB-9909:ATAC-seq_etoh_washout