title: Sample 62
description: Protocols: Single cells were isolated from the muscularis externa of Cx3cr1gfp/wt mice. Live Cx3cr1+ cells were FACS sorted into skirted 96 well hamilton plates for processing according to smartseq2 protocol. Cells were lysed for RNA extraction. Briefly, mRNA was reverse transcribed and cDNA was amplified via PCR. Amplification was carried out using KAPA HIFI Hot Start ReadyMix (Roche, 07958919001) and purification by magnetic beads (CleanNA CPCR). Quantity and quality of cDNA was assessed with the quantifluor RNA system (Promega E3310) and Agilent 2100 BioAnalyzer with a high-sensitivity chip. Library was prepared using the Nextera XT library prep and index kit (Illumina). cDNA was tagmented by transposase Tn5 and amplified with dual-index primers. Reagents were mixed together by the Echo 555 (Labcyte) and pooled-nextera XT libraries were purified.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-09-01
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-09-01
attributes.tag: External Id
attributes.value: SAMEA13003370
attributes.tag: INSDC center alias
attributes.value: KULEUVEN
attributes.tag: INSDC center name
attributes.value: KULEUVEN
attributes.tag: INSDC first public
attributes.value: 2022-09-01T00:14:03Z
attributes.tag: INSDC last update
attributes.value: 2022-09-01T00:14:03Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11453:Sample 62
attributes.tag: age
attributes.value: 56
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_type
attributes.value: macrophage
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: Cx3cr1-GFP/wt
attributes.tag: immunophenotype
attributes.value: Cx3cr1+
attributes.tag: organism part
attributes.value: small intestine smooth muscle
attributes.tag: sample name
attributes.value: E-MTAB-11453:Sample 62
attributes.tag: sex
attributes.value: not available
attributes.tag: single cell well quality
attributes.value: ok