title: Barley Rhizosphere sample AC41
description: Barley rhizosphere DNA from sample grown in soil conditioned with Morex (domesticated) barley under nitrogen limiting conditions . The soil was then used as a substrate for growth of Morex (domesticated) barley with a 25%N solution
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-09-01
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-09-01
attributes.tag: External Id
attributes.value: SAMEA110398782
attributes.tag: INSDC center alias
attributes.value: DUNDEE
attributes.tag: INSDC center name
attributes.value: University of Dundee
attributes.tag: INSDC first public
attributes.value: 2022-09-01T00:18:15Z
attributes.tag: INSDC last update
attributes.value: 2022-09-01T00:18:15Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: AC41
attributes.tag: broad-scale environmental context
attributes.value: rural area [ENVO:01000772]
attributes.tag: collection date
attributes.value: 2015-02-16
attributes.tag: environmental medium
attributes.value: farm soil [ENVO:00005749]
attributes.tag: geographic location (country and/or sea)
attributes.value: United Kingdom
attributes.tag: geographic location (latitude)
attributes.value: 56.45139
attributes.tag: geographic location (longitude)
attributes.value: -3.074722
attributes.tag: host common name
attributes.value: Barley
attributes.tag: host taxid
attributes.value: 4513
attributes.tag: local environmental context
attributes.value: research station [ENVO:00003919]
attributes.tag: mineral nutrient regimen
attributes.value: Morex (domesticated) barley plants were grown in a 0% N solution (0mM 2(Nh4)SO4; 0mM Ca(NO3)2; 0mM KNO3; 3mM MgSO4; 100µM FeEDTA+; 1mM KH2PO4; 0.025mM CaSO4+2H20; 0.08mM CaCl2; 0.25mM KCl) until early stem elongation, following which plants were removed.Morex (domesticated) barley plants were then grown in the soil with a 25% N solution (6.25mM 2(Nh4)SO4; 10mM Ca(NO3)2; 2.5mM KNO3; 3mM MgSO4; 100µM FeEDTA+; 1mM KH2PO4; 0.019mM CaSO4+2H20; 0.06mM CaCl2; 0.19mM KCl)
attributes.tag: pcr primers
attributes.value: 515F: GTGCCAGCMGCCGCGGTAA; 806R: GGACTACHVGGGTWTCTAAT
attributes.tag: project name
attributes.value: Barley NT
attributes.tag: sample name
attributes.value: AC41
attributes.tag: sequencing method
attributes.value: MiSeq [OBI:0002003]
attributes.tag: source material identifiers
attributes.value: AC41
attributes.tag: subspecific genetic lineage
attributes.value: Morex/Morex
attributes.tag: target gene
attributes.value: 16S rRNA
attributes.tag: target subfragment
attributes.value: V4