title: Syn3D71_10000SynHB3_Syn_control_lib6_Plate3_E12.fastq
description: To assess the specificity and sensitivity of sequencing and variant calling, the genes encoding msp2 of the P. falciparum PlasmoDB reference strains 3D7 (IC family, size variant of 473bp) and HB3 (FC27 family, size variant of 292bp) were chemically synthesized (GeneScript) and inserted into the pUC57 vector by EcoRV (GAT | ATC) blunt ligation. Multiclonal mock infections were prepared by diluting different molar ratios of pUC57-msp2_3D7 and pUC57-msp2_HB3 in water. Each molar ratio was assigned to a unique barcode combination
attributes.tag: ENA first public
attributes.value: 2022-06-09
attributes.tag: ENA last update
attributes.value: 2022-06-09
attributes.tag: External Id
attributes.value: SAMEA14511125
attributes.tag: INSDC center alias
attributes.value: KAROLINSKA INSTITUTET
attributes.tag: INSDC center name
attributes.value: KAROLINSKA INSTITUTET
attributes.tag: INSDC first public
attributes.value: 2022-06-09T00:14:54Z
attributes.tag: INSDC last update
attributes.value: 2022-06-09T00:14:54Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: Syn3D71_10000SynHB3_Syn_control_lib6_Plate3_E12
attributes.tag: common name
attributes.value: malaria parasite P. falciparum
attributes.tag: dev stage
attributes.value: Blood_stage
attributes.tag: host scientific name
attributes.value: Homo_sapiens
attributes.tag: isolation source
attributes.value: Blood_cell_pellets
attributes.tag: sample name
attributes.value: Syn3D71_10000SynHB3_Syn_control_lib6_Plate3_E12