title: Sample 8
description: Protocols: The yeast culture was treated and then centrifuged. The mutant yeast cells were grown overnight on SC+can media at 30 degree Celsius at 200 RPM. The cells were treated with metabolites (4NC and DP) for 3 days, and then selected for canavanine resistance on SC+can plates RNA isolation was done using TRIzol Reagent. The sample was homogenized with TRIzol Reagent, followed by addition of chloroform. The solution was allowed to separate into different layers, and the upper aqueous layer was transferred to isopropanol, in the presence of glycogen for precipitation. The precipitated RNA was washed with ethanol, and resuspended in nuclease-free water. DNAse treatment was done to remove DNA contamination. The pure RNA was again precipitated with ethanol, washed and resuspended in nuclease free water for downstream processing. SPRI Beads-based Size Selection
attributes.tag: ENA first public
attributes.value: 2022-06-09
attributes.tag: ENA last update
attributes.value: 2022-06-09
attributes.tag: External Id
attributes.value: SAMEA11298869
attributes.tag: INSDC center alias
attributes.value: Assistant Professor
attributes.tag: INSDC center name
attributes.value: Assistant Professor
attributes.tag: INSDC first public
attributes.value: 2022-06-09T08:13:20Z
attributes.tag: INSDC last update
attributes.value: 2022-06-09T08:13:20Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11179:Sample 8
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: baker's yeast
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: growth condition
attributes.value: YPD media with canavanine
attributes.tag: organism part
attributes.value: whole organism
attributes.tag: sample name
attributes.value: E-MTAB-11179:Sample 8
attributes.tag: strain
attributes.value: BY4741
attributes.tag: temperature
attributes.value: 30