title: H8N8 CAF 1
description: Protocols: H8N8 cells were treated with CAF (312,5 ng/ml cyclophosphamide, 15,6 ng/ml doxorubicin and 312,5 ng/ml 5-FU) in 10 cm plates for 48 hours in biological triplicates. Total RNA was extracted from G-2 cells with Qiazol (Qiagen) following the manufacturer's recommendations. mRNA sequencing libraries were generated with the NEXTflex™ Rapid Illumina Directional Kit according to the manufacturer's instructions. The size of the generated libraries was estimated on High Sensitivity DNA chip (Agilent) using a Bioanalyser 2100 (Agilent).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-09-01
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-09-01
attributes.tag: External Id
attributes.value: SAMEA12505627
attributes.tag: INSDC center alias
attributes.value: Department of Gynecology and Obstetrics Molecular Gynecology Laboratory
attributes.tag: INSDC center name
attributes.value: Department of Gynecology and Obstetrics Molecular Gynecology Laboratory
attributes.tag: INSDC first public
attributes.value: 2022-09-01T12:16:05Z
attributes.tag: INSDC last update
attributes.value: 2022-09-01T12:16:05Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11344:H8N8 CAF 1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: H8N8
attributes.tag: cell_type
attributes.value: breast invasive ductal carcinoma cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: disease
attributes.value: breast cancer
attributes.tag: genotype
attributes.value: WAP-T-NP8; WAP-mutp53R270H
attributes.tag: organism part
attributes.value: breast
attributes.tag: replicate
attributes.value: 1
attributes.tag: sample name
attributes.value: E-MTAB-11344:H8N8 CAF 1
attributes.tag: stimulus
attributes.value: CAF (312,5 ng/ml cyclophosphamide, 15,6 ng/ml doxorubicin and 312,5 ng/ml 5-Fluorouracil)
attributes.tag: strain
attributes.value: BALB/c