title: Sample_2
description: Protocols: At least 50 seedlings were pooled from three independent petri dishes for RNA extraction. For treatment, 7 days-old seedlings were subjected to changes in temperature only during the light period. Long-term heat consisted of an entrainment treatment of 5 heat cycles (day 37C, night 21C, 12/12 h; final age 12 days). This was followed by 3 days of recovery at 21C in recovery samples (age at harvest 15 days). Total RNA was isolated from biologically duplicated samples using the the Plant-RNAeasy kit (Invitrogen), following provider's instructions. Libraries were prepared with the corresponding Illumina library preparation kit, following provider's instructions.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-09-01
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-09-01
attributes.tag: External Id
attributes.value: SAMEA10157635
attributes.tag: INSDC center alias
attributes.value: IFEVA (CONICET-UBA), Facultad de Agronomia (FAUBA), Universidad de Buenos Aires (UBA)
attributes.tag: INSDC center name
attributes.value: IFEVA (CONICET-UBA), Facultad de Agronomia (FAUBA), Universidad de Buenos Aires (UBA)
attributes.tag: INSDC first public
attributes.value: 2022-09-01T00:13:45Z
attributes.tag: INSDC last update
attributes.value: 2022-09-01T00:13:45Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-10928:Sample_2
attributes.tag: age
attributes.value: 15
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: thale cress
attributes.tag: developmental stage
attributes.value: seedling development stage
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: whole organism
attributes.tag: sample name
attributes.value: E-MTAB-10928:Sample_2
attributes.tag: stimulus
attributes.value: recovery after long term heat stress
attributes.tag: ecotype
attributes.value: Col-0