title: Pancreas_Meso_R2
description: Protocols: Tissues were digested and CD45-CD31-Epcam-PDPN+PDGFRA+ (fibroblasts) or CD45-CD31-Epcam-PDPN+PDGFRA- mesothelial cells were sorted directly into TRIZOL. RNA from FACS sorted cells was sourced from the following mouse tissues: Liver, Lung, Lymph Node, Spleen, Bone, Pancreas, Omentum, Mesentery, Epididymal, Inguinal Adipose, Brown Adipose Tissue. RNA was isolated as described (Painter et al., 2011, J. Imm.) or at Expression Analysis, Inc. Paired-end RNA-seq libraries were constructed using the SMARTer Low Input RNA Kit (Takara.
attributes.tag: ENA first public
attributes.value: 2021-05-13
attributes.tag: ENA last update
attributes.value: 2021-05-13
attributes.tag: External Id
attributes.value: SAMEA8491899
attributes.tag: INSDC center name
attributes.value: GENENTECH INC.
attributes.tag: INSDC first public
attributes.value: 2021-05-04T00:42:28Z
attributes.tag: INSDC last update
attributes.value: 2021-03-31T14:33:20Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-10324:Pancreas_Meso_R2
attributes.tag: age
attributes.value: 6 to 12
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: mesothelial cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: organism part
attributes.value: pancreas
attributes.tag: phenotype
attributes.value: CD45-CD31-Epcam-PDPN+PDGFRA-
attributes.tag: sample name
attributes.value: E-MTAB-10324:Pancreas_Meso_R2
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: C57BL6/J