title: RNA-seq_dex_dex_rep1
description: Protocols: RNA was isolated from 1 million cells using the RNeasy kit from Qiagen. A549 cells were grown to approximately 90% confluence according to standard protocols in DMEM medium with 5% FBS. Cells were treated with either 100 nM dexamethasone or 0.1% ethanol. After 4 hours, cells were washed 2x with PBS and cultured in hormone-free medium for 24 hours, after which cells were treated again for 4 hours with either 100 nM dexamethasone or 0.1% ethanol. Ribosomal RNA was depleted using the KAPA RNA HyperPrep with RiboErase (Roche #08098131702). Sequencing libraries were prepared using KAPA RNA HyperPrep with RiboErase (Roche #08098131702).
attributes.tag: ENA first public
attributes.value: 2021-05-12
attributes.tag: ENA last update
attributes.value: 2021-05-12
attributes.tag: External Id
attributes.value: SAMEA7725468
attributes.tag: INSDC center name
attributes.value: MPIMG
attributes.tag: INSDC first public
attributes.value: 2021-05-03T00:22:16Z
attributes.tag: INSDC last update
attributes.value: 2020-12-16T15:09:16Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-9923:RNA-seq_dex_dex_rep1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: A549
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: carcinoma
attributes.tag: organism part
attributes.value: lung
attributes.tag: sample name
attributes.value: E-MTAB-9923:RNA-seq_dex_dex_rep1