title: 16S rRNA gene sequencing of cecal and colonic contents derived from SPF C3-/- pups (n=5)
description: Total genomic DNA from cecal and colonic contents derived from 20-day-old specific germ free (SPF) C3-/- mice was extracted using QIAamp DNA Stool Mini Kit (QIAGEN). The V4 region of 16S rRNA gene were amplified with the published primer pair of 515F and 806R, followed by sequencing using an Ion S5 XL platform and 400 bp single-end reads were generated.
attributes.tag: strain
attributes.value: C57BL/6 background
attributes.tag: collection_date
attributes.value: NA
attributes.tag: env_broad_scale
attributes.value: NA
attributes.tag: env_local_scale
attributes.value: NA
attributes.tag: env_medium
attributes.value: NA
attributes.tag: geo_loc_name
attributes.value: USA: Baltimore
attributes.tag: host
attributes.value: Mus musculus
attributes.tag: isol_growth_condt
attributes.value: 29420293
attributes.tag: lat_lon
attributes.value: NA
attributes.tag: host_age
attributes.value: 20 days
attributes.tag: host_disease
attributes.value: none, healthy
attributes.tag: host_genotype
attributes.value: C3-
attributes.tag: host_tissue_sampled
attributes.value: cecal and colonic contents
attributes.tag: samp_size
attributes.value: 5
attributes.tag: sample type
attributes.value: 16S rRNA gene sequencing
attributes.tag: BioSampleModel
attributes.value: MIMARKS.specimen
attributes.tag: BioSampleModel
attributes.value: MIGS/MIMS/MIMARKS.host-associated