title: Sample 6
description: Protocols: BALB/c mice were injected with CFZ (16 mg/kg, i.v.), CFZ and atRA (CFZ: 16 mg/kg i.v., atRA: 1 mg/kg i.v.) or Captisol (vehicle, i.v.) (2 mice per cohort). ECGs were recorded under anesthesia at the end of 1h treatment and the mice were sacrificed by cervical dislocation. Euthanized animals were perfused with 20 ml of PBS and small heart tissue pieces were digested three times for 20 min at 37°C with 120 µg/ml collagenase P (Roche), 25 µg/ml DNase I (Applichem, Germany) in medium (RPMI-1640 containing 5% FCS (Sigma-Aldrich, Switzerland) and10 mM HEPES (Sigma-Aldrich, Switzerland). To eliminate remaining erythrocytes, the cell suspensions were incubated with MACS anti-Ter119 microbeads (Miltenyi Biotec, Germany) and passed through a MACS LS column (Miltenyi Biotec, Germany). Unbound single cell suspensions were stained with Live/Dead Fixable e780 Aqua staining. Live cells were sorted with a BD FACS Melody cell sorter (BD Biosciences, CA, USA). cDNA library generation was performed following the established commercial protocol for Chromium Single Cell 3' Reagent Kit (v3 Chemistry).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-09-01
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-09-01
attributes.tag: External Id
attributes.value: SAMEA10289709
attributes.tag: INSDC center alias
attributes.value: Institute of Immunobiology, Kantonsspital St. Gallen, St. Gallen, Switzerland
attributes.tag: INSDC center name
attributes.value: Institute of Immunobiology, Kantonsspital St. Gallen, St. Gallen, Switzerland
attributes.tag: INSDC first public
attributes.value: 2022-09-01T00:13:47Z
attributes.tag: INSDC last update
attributes.value: 2022-09-01T00:13:47Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11028:Sample 6
attributes.tag: age
attributes.value: 8 to 12
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: individual
attributes.value: mouse 6
attributes.tag: organism part
attributes.value: heart
attributes.tag: sample name
attributes.value: E-MTAB-11028:Sample 6
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: BALB/c