title: V6_1_in_10000_synthetic_lib5_Plate1_F5.fastq
description: To assess the specificity and sensitivity of sequencing and variant calling, the genes encoding msp2 of the P. falciparum reference strains 3D7 (IC1 family, size variant of 473bp), HB3 (FC27 family, size variant of 292bp), CD01 (FC27 family, size variant of 292bp), Dd2 (FC27 family, size variant of 412bp), SN01 (FC27 family, size variant of 292bp), KE01 (FC27 family, size variant of 376bp), SD01 (IC1 family, size variant of 434bp), GN01 (IC1 family, size variant of 539bp), 7G8 (IC1 family, size variant of 485bp) and GB4 (IC1 family, size variant of 533bp) were chemically synthesized (GeneScript) and inserted into the pUC57 vector by EcoRV (GAT | ATC) blunt ligation. Multiclonal mock infections were prepared by diluting different molar ratios of the variants in water in complexities of infection ranging from 1 to 10. Each mock infection was assigned to a unique barcode combination
attributes.tag: ENA first public
attributes.value: 2022-06-09
attributes.tag: ENA last update
attributes.value: 2022-06-09
attributes.tag: External Id
attributes.value: SAMEA14510840
attributes.tag: INSDC center alias
attributes.value: KAROLINSKA INSTITUTET
attributes.tag: INSDC center name
attributes.value: KAROLINSKA INSTITUTET
attributes.tag: INSDC first public
attributes.value: 2022-06-09T00:14:51Z
attributes.tag: INSDC last update
attributes.value: 2022-06-09T00:14:51Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: V6_1_in_10000_synthetic_lib5_Plate1_F5
attributes.tag: common name
attributes.value: malaria parasite P. falciparum
attributes.tag: dev stage
attributes.value: Blood_stage
attributes.tag: host scientific name
attributes.value: Homo_sapiens
attributes.tag: isolation source
attributes.value: Blood_cell_pellets
attributes.tag: sample name
attributes.value: V6_1_in_10000_synthetic_lib5_Plate1_F5