title: RAT2-C
description: Protocols: After 4 weeks of regular exercise, rats underwent brief anesthesia and of blood from the tail vein was taken and immediately put into K3 EDTA tubes and centrifuged for 15 min at 2000 x g at 4 degrees C. This step was repeated for the same animals immediately after 1h of running. On the same day, a blood sample was collected from the sedentary rats following the same protocol. Plasma was collected and immediately frozen for further EV isolation. RNA was extracted from plasma EVs using miRNeasy micro kit (Qiagen, USA) according to the manufacturer's protocol. The concentration and quality of the obtained RNA were analyzed on Agilent Bioanalyzer with Agilent RNA 6000 Pico chip (Agilent Technologies, Germany). RNA libraries were constructed using CleanTag® Small RNA Library Prep Kit (Trilink Biotechnologies, USA), the quality and concentration of obtained libraries were analyzed on Agilent Bioanalyzer using Agilent High Sensitivity DNA chip (Agilent Technologies, Germany). The libraries were cleaned using Blue Pippin DNA Size Selection with 3% gel Blue Pippin Cassette (Sage Science, USA) setting tight target length to 140 bp thus selecting fragments with size in tight range to 140 bp (133–147 bp).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2021-12-28
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2021-12-28
attributes.tag: External Id
attributes.value: SAMEA10273696
attributes.tag: INSDC center alias
attributes.value: Latvian Biomedical Research and Study Centre
attributes.tag: INSDC center name
attributes.value: Latvian Biomedical Research and Study Centre
attributes.tag: INSDC first public
attributes.value: 2021-12-28T04:10:28Z
attributes.tag: INSDC last update
attributes.value: 2021-12-28T04:10:28Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11020:RAT2-C
attributes.tag: age
attributes.value: 12
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: Norway rat
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: environmental history
attributes.value: sedentary lifestyle
attributes.tag: fraction
attributes.value: extracellular vesicular exosome
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: individual
attributes.value: RAT2
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: blood
attributes.tag: sample name
attributes.value: E-MTAB-11020:RAT2-C
attributes.tag: sampling time point
attributes.value: not applicable
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: F344