title: do16076_1
description: Protocols: Nuclei were isolated from frozen livers from young C57BL/6 mice (3 month old) using the protocol described in Krishnaswami et al. 2016 (Nature Protocols, 11: 499-524). Nuclei were purified using Iodixanol gradient (Optiprep, D1556, Sigma Aldrich Chemie). Nuclei were stained with Hoechst and FACS sorted according to their ploidy level into tubes containing PBS/BSA 0,05%. The 2n and 4n nuclei samples were loaded in a Chromium Single Cell A Chip (10X Genomics, PN-120236) and were processed through the Chromium Single Cell Platform using the Chromium Single Cell 3′ Library and Gel Bead Kit v2 (10X Genomics, PN-120237) as per manufacturers instructions. After cDNA amplification, 25% of the cDNA was processed for Library preparation (10X Genomics, PN-120237) as per the manufacturer's instructions. The final libraries were quantified and pooled for sequencing.
attributes.tag: ENA first public
attributes.value: 2021-05-07
attributes.tag: ENA last update
attributes.value: 2021-03-17
attributes.tag: External Id
attributes.value: SAMEA8268095
attributes.tag: INSDC center alias
attributes.value: Helmholtz Pioneer Campus
attributes.tag: INSDC center name
attributes.value: Helmholtz Pioneer Campus
attributes.tag: INSDC first public
attributes.value: 2021-05-07T00:27:00Z
attributes.tag: INSDC last update
attributes.value: 2021-03-17T08:13:19Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-10223:do16076_1
attributes.tag: age
attributes.value: 19.2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: individual
attributes.value: 15/028687
attributes.tag: organism part
attributes.value: liver
attributes.tag: ploidy
attributes.value: 2n
attributes.tag: sample name
attributes.value: E-MTAB-10223:do16076_1
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: C57BL6/J