title: Sample 1
description: Protocols: Single cells from aortas were digested in 5% FBS/RPMI containing 450 U/ml collagenase I, 125 U/ml collagenase XI 60 U/ml hyaluronidase and 60 U/ml DNase I for 50 minutes at 37°C. Cells were retrieved by passing tissue pieces through a 70 um cell strainer and were pooled from 9 mice in each genotype. Unspecific binding was blocked by incubating cells with murine anti-CD16/32 antibody. Cells were stained with antibodies against CD45, CD11b and CD64 and LIVE/DEAD viability dye. Live CD45+ CD11b+ cells were sorted for sequencing. Library generation for 10x Genomics v2 chemistry was performed following the Chromium Single Cell 3ʹ Reagents Kits (User Guide: CG00052). Quantification of cDNA and library construction was performed using a Qubit dsDNA HS Assay Kit (ThermoFisher Scientific) and high-sensitivity DNA tapestation (Agilent).
attributes.tag: ENA first public
attributes.value: 2021-10-28
attributes.tag: ENA last update
attributes.value: 2021-10-28
attributes.tag: External Id
attributes.value: SAMEA9425863
attributes.tag: INSDC center alias
attributes.value: Kennedy Institute of Rheumatology University of Oxford
attributes.tag: INSDC center name
attributes.value: Kennedy Institute of Rheumatology University of Oxford
attributes.tag: INSDC first public
attributes.value: 2021-10-28T05:45:22Z
attributes.tag: INSDC last update
attributes.value: 2021-10-28T05:45:22Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-10746:Sample 1
attributes.tag: age
attributes.value: 20
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell type
attributes.value: myeloid cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: diet
attributes.value: high fat diet
attributes.tag: disease
attributes.value: atherosclerosis
attributes.tag: genotype
attributes.value: ApoE-/-
attributes.tag: growth condition
attributes.value: high fat diet for 12 weeks
attributes.tag: immunophenotype
attributes.value: CD45+CD11b+
attributes.tag: individual
attributes.value: pool of 9 mice
attributes.tag: organism part
attributes.value: aorta
attributes.tag: sample name
attributes.value: E-MTAB-10746:Sample 1
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: C57BL/6J