description: Ribosomal RNA depleted RNA was isolated, purified by using RiboMinus™ Eukaryote Kit (Invitrogen, #A10837-08) and chemically shredded into ~100-nt fragments by using RNA fragmentation reagents (Invitrogen, #AM8740).  1 ug RNA of the fragments was used as the input (the rest for m6A-MeRIP-Seq analysis) and used to construct libraries with TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA). Sequencing was carried out on Illumina HiSeq 4000 according to the manufacturer's instructions with single-end 50-bp read length.
attributes.tag: strain
attributes.value: BALB/c
attributes.tag: isolate
attributes.value: MeRIP-Input-Ctrl-1
attributes.tag: breed
attributes.value: not applicable
attributes.tag: cultivar
attributes.value: not applicable
attributes.tag: ecotype
attributes.value: not applicable
attributes.tag: age
attributes.value: not applicable
attributes.tag: dev_stage
attributes.value: not applicable
attributes.tag: sex
attributes.value: not applicable
attributes.tag: tissue
attributes.value: Intetinal epithelial cells
attributes.tag: cell_line
attributes.value: IEC4.1
attributes.tag: cell_type
attributes.value: Intestinal epithelial cells
attributes.tag: sample_type
attributes.value: Cell culture
attributes.tag: treatment
attributes.value: The IEC 4.1 murine intestinal epithelial cells were grown to 80% confluence for two groups: non-infected control (Ctrl) and Cryptosporidium parvum (CP)-infected group. Cells for CP infection were exposed to C. parvum infection for 24h. Cells were then collected and RNA isolated for m6A-MeRIP-Seq analysis.
attributes.tag: BioSampleModel
attributes.value: Model organism or animal