description: A library of single-codon substitutions in AadB was constructed using inverse PCR. The library was transformed into NEB 5-alpha F'Iq cells. We diluted uninduced, exponentially-growing cultures of the libraries mixed with wildtype AadB into LB media containing IPTG. Cultures were allowed to grow for ~10 generations at 37°C with a 32-fold dilution after ~5 generations of growth. We plasmid prepped and determined the allele frequencies of the generation 10, replica 2 sample using deep sequencing. Data for codons 1-178 was used from these files. This was used as Replica 2 for regions 1 (codons 1-89) and 2 (codons 90-178).
attributes.tag: strain
attributes.value: NEB 5-alpha F'Iq
attributes.tag: isolation_source
attributes.value: not applicable
attributes.tag: collection_date
attributes.value: 2020-06
attributes.tag: geo_loc_name
attributes.value: USA: Maryland
attributes.tag: sample_type
attributes.value: cell culture
attributes.tag: temp
attributes.value: 37°C
attributes.tag: replicate
attributes.value: sequencing run 6
attributes.tag: generation
attributes.value: 10
attributes.tag: BioSampleModel
attributes.value: Microbe, viral or environmental