description: Protocols: Total RNA was isolated from hMLOs using TRIzol Reagent (Thermo Fisher). DNA contaminants were digested with DNASE I (Ambion). For RNA-seq library preparation, total RNA was further purified using the Purelink RNA Mini kit (Ambion). 1.5 ug total RNA was used for library construction according to manufacturer's protocol (TruSeq stranded mRNA Library Preparation Kit, Illumina).
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism
attributes.value: Homo sapiens
attributes.tag: cell_line
attributes.value: H9
attributes.tag: developmental stage
attributes.value: embryo stage
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: inner cell mass
attributes.tag: progenitor cell type
attributes.value: embryonic stem cell
attributes.tag: growth condition
attributes.value: midbrain-like organoid culture
attributes.tag: sampling time point
attributes.value: 90
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2021-05-28
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2021-08-13