attributes.tag: isolate
attributes.value: To obtain a single-cell suspension, 5-7 organoids at D30 were randomly selected into a 1.5 ml EP tube with 1 ml of TrypLE (Life Technologies) and incubated in a 37 degrees Celsius incubator for 40 min with gentle agitation every 5-8 min. The organoids were then washed with DPBS (Life Technologies) 3 times and gently dissociated into single cells with a 200 microliter pipette.
attributes.tag: age
attributes.value: 30 days in vitro
attributes.tag: biomaterial_provider
attributes.value: WiCell
attributes.tag: sex
attributes.value: female
attributes.tag: tissue
attributes.value: human brain organoids
attributes.tag: cell_line
attributes.value: H9
attributes.tag: BioSampleModel
attributes.value: Human