title: 13_Psychrobacter sp002366815_isolate_B
organism: Psychrobacter sp. CCS180
attributes.tag: strain
attributes.value: CCS180
attributes.tag: collection_date
attributes.value: 2020-08-20
attributes.tag: env_broad_scale
attributes.value: anthropogenic environmental material[ENVO:0010001]
attributes.tag: env_local_scale
attributes.value: cheese product[ENVO:00002189]
attributes.tag: env_medium
attributes.value: cheese rind biofilm
attributes.tag: geo_loc_name
attributes.value: USA
attributes.tag: isol_growth_condt
attributes.value: CCS180 was isolated from the rind of a washed-rind cheese by plating on PCAMS (5 g/L tryptone, 2.5 g/L yeast extract, 1g/L dextrose, 1 g/L whole milk powder, 10 g/L sodium chloride and 15 g/L agar) containing 100 g/ml cycloheximide to select against the fungal community members and incubation at 15 C for 4 days and an additional 3 days at room temperature on the bench top. A single colony was streak-purified on PCAMS three times. Overnight cultures of the isolate were grown for 24 hours at room temperature in LB Miller broth and flash-frozen in liquid nitrogen in PBS+20% glycerol. Glycerol stocks were stored at -80 C.
attributes.tag: lat_lon
attributes.value: 32.8801 N 117.2340 W
attributes.tag: num_replicons
attributes.value: n.a.
attributes.tag: ref_biomaterial
attributes.value: n.a.
attributes.tag: BioSampleModel
attributes.value: MIGS.ba