organism: Homo sapiens
attributes.tag: isolate
attributes.value: P4
attributes.tag: synthetic
attributes.value: FALSE
attributes.tag: sex
attributes.value: male
attributes.tag: age
attributes.value: 35
attributes.tag: age_event
attributes.value: Enrollment
attributes.tag: population
attributes.value: North America
attributes.tag: ethnicity
attributes.value: Unknown
attributes.tag: race
attributes.value: Caucasian
attributes.tag: strain
attributes.value: N/A
attributes.tag: linked_subjects
attributes.value: N/A
attributes.tag: link_type
attributes.value: N/A
attributes.tag: study_group_description
attributes.value: COVID-19 convalescent subjects
attributes.tag: disease
attributes.value: COVID-19
attributes.tag: disease_length
attributes.value: 7 days
attributes.tag: disease_stage
attributes.value: Convalescence
attributes.tag: prior_therapies
attributes.value: N/A
attributes.tag: immunogen
attributes.value: SARS-CoV-2
attributes.tag: treatment
attributes.value: I.V. fluid maintenance
attributes.tag: medical_history
attributes.value: N/A
attributes.tag: sample_type
attributes.value: Whole blood
attributes.tag: tissue
attributes.value: Blood
attributes.tag: anatomic_site
attributes.value: median cubital vein
attributes.tag: health_state
attributes.value: COVID-19 convalesence
attributes.tag: collection_time_point_relative
attributes.value: 19
attributes.tag: collection_time_point_reference
attributes.value: Primary infection symptom onset
attributes.tag: biomaterial_provider
attributes.value: University of Texas at Austin
attributes.tag: tissue_processing
attributes.value: Lonza lymphocyte separation medium
attributes.tag: cell_type
attributes.value: B cell
attributes.tag: cell_phenotype
attributes.value: N/A
attributes.tag: single_cell
attributes.value: True
attributes.tag: cell_number
attributes.value: 150000
attributes.tag: cells_per_reaction
attributes.value: 150000
attributes.tag: cell_storage
attributes.value: True
attributes.tag: cell_quality
attributes.value: 0.9
attributes.tag: cell_isolation
attributes.value: None
attributes.tag: cell_processing_protocol
attributes.value: PBMCs were isolated using the Lonza lymphocyte separation medium and cryopreserved until ready for processing. PBMCs were then thawed, incubated with DNaseI, washed, and resuspended in PBS. 2 million PBMCs (~150,000 B cells) were then run through the custom made Flow Focusing Device to encapsulate single cells in oil and water emulsions containing oligo-d(T) magnetic beads and lysis buffer. Within the emulsions, mRNA was captured following cell lysis. Emulsions were then broken using diethyl ether, and the magnetic oligo-d(T) beads were captured, washed, and resuspended in overlap-extension (OE)/RT-PCR mix. Single beads were then re-emulsified and BCR heavy and light chain mRNA transcripts from single cells were physically linked, reverse-transcribed, and amplified within the emulsions.
attributes.tag: BioSampleModel
attributes.value: Human