title: Sample 2
description: Protocols: Maize leaves were collected at 3rd leaf stage after by 0.3 M mannitol treatment of roots for 4 h for RNA extraction. Maize seedlings were grown in Hogland's nutrient solution at 400 mol m-2 s-1 photosynthetically active radiation, a 14/10-h day/night cycle and a day/night temperature of 28oC/22oC (relative humidity of 75%). When the second leaves were fully expanded, seedlings were transferred in 0.3 M mannitol in Hogland's solution for 4 h and then sampled for RNA extraction. CTAB PBIOZOL and ethanol precipitation protocol was used for extraction and purification of total RNA from maize leaf tissue. mRNA was fragmented and enriched with by oligo-dT beads magnetic beads. Sequencing library was amplified with the broken mRNA as the templates to synthesize cDNA. The cDNA templates were purified with the kit and enriched by PCR.
attributes.tag: ENA first public
attributes.value: 2022-02-28
attributes.tag: ENA last update
attributes.value: 2022-02-28
attributes.tag: External Id
attributes.value: SAMEA12311262
attributes.tag: INSDC center alias
attributes.value: HENAN AGRICULTURAL UNIVERSITY
attributes.tag: INSDC center name
attributes.value: HENAN AGRICULTURAL UNIVERSITY
attributes.tag: INSDC first public
attributes.value: 2022-02-28T12:35:28Z
attributes.tag: INSDC last update
attributes.value: 2022-02-28T12:35:28Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11331:Sample 2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cultivar
attributes.value: inbred line
attributes.tag: developmental stage
attributes.value: 3rd-leaf stage
attributes.tag: genotype
attributes.value: Chang7-2t
attributes.tag: organism part
attributes.value: leaf lamina
attributes.tag: sample name
attributes.value: E-MTAB-11331:Sample 2
attributes.tag: sampling site
attributes.value: 1st leaf
attributes.tag: stimulus
attributes.value: normal watering