title: Flox18
description: Protocols: Liver biopsies were harvested from control flox and Hmgb1 liver knockout mice in chow diet or after fasting/refeeding challenge Flash-frozen liver biopsies were sent to Active Motif to perform the ATAC-seq assay. The tissue was manually dissociated, isolated nuclei were quantified using a hemocytometer, and 100,000 nuclei were tagmented as previously described (58), with some modifications based on (59) using the enzyme and buffer provided in the Nextera Library Prep Kit (Illumina). Tagmented DNA was then purified using the MinElute PCR purification kit (Qiagen), amplified with 10 cycles of PCR, and purified using Agencourt AMPure SPRI beads (Beckman Coulter). Resulting material was quantified using the KAPA Library Quantification Kit for Illumina platforms (KAPA Biosystems).
attributes.tag: ENA first public
attributes.value: 2022-03-31
attributes.tag: ENA last update
attributes.value: 2022-03-31
attributes.tag: External Id
attributes.value: SAMEA13409739
attributes.tag: INSDC center alias
attributes.value: INSERM U1301 CNRS 5070 EFS/Univ Paul Sabatier
attributes.tag: INSDC center name
attributes.value: INSERM U1301 CNRS 5070 EFS/Univ Paul Sabatier
attributes.tag: INSDC first public
attributes.value: 2022-03-31T00:18:29Z
attributes.tag: INSDC last update
attributes.value: 2022-03-31T00:18:29Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11542:Flox18
attributes.tag: age
attributes.value: 18 to 20
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: diet
attributes.value: fast for 6 hours and refeed for 8 hours
attributes.tag: genotype
attributes.value: HMGB1fl/fl
attributes.tag: organism part
attributes.value: liver
attributes.tag: sample name
attributes.value: E-MTAB-11542:Flox18
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: C57BL/6J