title: Patient 7
description: Protocols: RNA was isolated from temporal cortex tissue of 10 FTLD brains. 50 mg of flash-frozen tissue was homogenised in 700 µl of Qiazol (Qiagen) using a TissueRuptor II (Qiagen). Chloroform was added and RNA subsequently extracted following the spin-column protocol from the miRNeasy kit with DNase digestion (Qiagen). RNA was eluted off the column in 50 µl of RNAse-free water. Purified RNA was reverse transcribed with Superscript IV (Thermo Fisher Scientific) using either sequence specific primers containing sample-specific barcodes or random hexamers, following the manufacturer recommendations. Unique molecular identifiers (UMIs) and part of the P5 Illumina sequence were added either during first- or second-strand-synthesis (with Phusion HF 2x Master Mix) respectively. Three rounds of nested PCR using Phusion HF 2x Master Mix (New England Biolabs) were used to obtain highly specific amplicons for the UNC13A cryptic, followed by gel extraction and a final round of PCR in which the full length P3/P5 Illumina sequences were added.
attributes.tag: ENA first public
attributes.value: 2022-02-28
attributes.tag: ENA last update
attributes.value: 2022-02-28
attributes.tag: External Id
attributes.value: SAMEA11549296
attributes.tag: INSDC center alias
attributes.value: UNIVERSITY COLLEGE LONDON
attributes.tag: INSDC center name
attributes.value: UNIVERSITY COLLEGE LONDON
attributes.tag: INSDC first public
attributes.value: 2022-02-28T00:30:53Z
attributes.tag: INSDC last update
attributes.value: 2022-02-28T00:30:53Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-10237_2:Patient 7
attributes.tag: age
attributes.value: 68
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: FTLD-TDPA
attributes.tag: organism part
attributes.value: temporal cortex
attributes.tag: sample name
attributes.value: E-MTAB-10237_2:Patient 7
attributes.tag: sex
attributes.value: male