title: SCRB-seq
description: Protocols: HEK293FT cells were dissociated using TrypLE. Cells were FACS sorted and lysed into 5 ul of Phusion HF (1/500 diluted) buffer supplemented with 0.025 pg of 3'-molecular spike-in and lysate was used for library preparation without nucleic acid extraction. Libraries were prepared in 3 variations of the SCRB-seq protocol. (1) standard protocol as described by Soumillon et al., 2014 (2) as (1) but omitting the ExonucleaseI step prior to cDNA amplification and (3) PCR master-mix was added to each well individually, omitting both the pooling and Exonuclease I steps.
attributes.tag: ENA first public
attributes.value: 2022-02-16
attributes.tag: ENA last update
attributes.value: 2022-02-16
attributes.tag: External Id
attributes.value: SAMEA8657190
attributes.tag: INSDC center alias
attributes.value: Karolinska Institutet Department of Cell & Molecular Biology
attributes.tag: INSDC center name
attributes.value: Karolinska Institutet Department of Cell & Molecular Biology
attributes.tag: INSDC first public
attributes.value: 2022-02-16T12:28:50Z
attributes.tag: INSDC last update
attributes.value: 2022-02-16T12:28:50Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-10372:SCRB-seq
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: HEK293T
attributes.tag: common name
attributes.value: human
attributes.tag: sample name
attributes.value: E-MTAB-10372:SCRB-seq