title: AEL-33 ChIPseq H3K27ac
description: Protocols: Cell lines were collected at 24h and 144h post doxycycline (DOX) withdrawal or maintained with DOX in media culture. Patient derived xenograft (PDX) cells of AEL patients were obtained from bone marrow and spleen of NSG recipient mice. Cells were immediately fixed in 1%PFA (and 0.2mM of DSG for ChIPseq-EP300) and quenched using Glycine (0.125M). After cells lysis, chromatin was sheared by sonication (Covaris apparatus) and use for immunoprecipitation. Next washing, chromatin was purified using IPure Kit V2 (Diagenode, Cat No C03010012) following manufacturer's instructions. DNA fragments were amplified by PCR with indexed paired-end adaptors (Illumina). PCR purification was performed using AMPure XP magnetic beads (Beckman Coulter)
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2021-12-30
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2021-12-30
attributes.tag: External Id
attributes.value: SAMEA8569281
attributes.tag: INSDC center alias
attributes.value: INSERM Institut Gustave Roussy
attributes.tag: INSDC center name
attributes.value: INSERM Institut Gustave Roussy
attributes.tag: INSDC first public
attributes.value: 2021-12-30T00:11:42Z
attributes.tag: INSDC last update
attributes.value: 2021-12-30T00:11:42Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-10346:AEL-33 ChIPseq H3K27ac
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: AEL-33 xenograft
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: acute erythroleukemia
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: hematopoietic system
attributes.tag: phenotype
attributes.value: wild type
attributes.tag: sample name
attributes.value: E-MTAB-10346:AEL-33 ChIPseq H3K27ac