title: G_Evolvex26_A
description: Protocols: Samples were collected at mid-exponential growth phase for transcriptomics and proteomics. For total RNA, 20 mL of culture broth was collected in a 50 mL Falcon® tube filled with ice and immediately centrifuged at 10.000rpm for 2 min at 0 °C. After centrifugation, cell pellet were snap-frozen in liquid nitrogen and kept at -80°C until extraction. Pre-cultures were prepared by cultivating a single colony from a selective YPD plate of each yeast strain in YPD medium until mid-exponential phase (~16 h). Pre-cultured cells were centrifuged, washed two times with distilled water, and inoculated in evolution niche media or natural wine must at initial OD600 of 0.1. Cultivations were performed in triplicates. Total RNA was isolated with RNAeasy kit (Qiagen) following manufacturer recommendations. To remove DNA contamination, samples were digested with Turbo DNAse (Invitrogen Ambion) followed by RNA clean-up (RNAeasy kit, Qiagen). RNA library was prepared using the NEBNext® Ultra™ II Directional RNA Library Preparation Kit for Illumina: polyA transcripts capture. Briefly, barcoded stranded mRNA-seq libraries were prepared from high quality total RNA samples (~200 ng/sample) using the NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs (NEB), Ipswich, MA, USA) implemented on the liquid handling robot Beckman i7.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2021-12-30
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2021-12-30
attributes.tag: External Id
attributes.value: SAMEA7837933
attributes.tag: INSDC center alias
attributes.value: Structural and Computational Biology,European Molecular Biology Laboratory - EMBL
attributes.tag: INSDC center name
attributes.value: Structural and Computational Biology,European Molecular Biology Laboratory - EMBL
attributes.tag: INSDC first public
attributes.value: 2021-12-30T00:11:37Z
attributes.tag: INSDC last update
attributes.value: 2021-12-30T00:11:37Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-10019:G_Evolvex26_A
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: baker's yeast
attributes.tag: growth condition
attributes.value: evolution niche glycerol
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: sample name
attributes.value: E-MTAB-10019:G_Evolvex26_A
attributes.tag: strain
attributes.value: Evolvex26