title: SCC327 Colon 18-1417N 2
description: Protocols: original samples 1-6 were obtained from patient biopsies, expanded and passaged in vitro. Samples 7-8 were derived from induced pluripotent human foreskin fibroblast cells Organoids from Matrigel domes (2 biological replicates per sample)were dislodged and pelleted at 500g for 5 minutes. Supernatant was aspirated and pellets were resuspended in 1 mL TrypLE express using repeated pipetting and incubated at 37 C for 10 min. Samples were pelleted 500g for 5 min. Supernatant was aspirated and resuspended in 400 uL TRI reagent then transferred to a microcentrifuge tube and pelleted 13000g for 10 min. 350 uL supernatant was transferred to a new microcentrifuge tube. Equal volume of 100% ethanol was added and mixed. Samples were column-purified following protocol of Zymo DirectZol RNA miniprep. Poly-A selection was performed on bulk RNA and adapters were ligated for library preparation
attributes.tag: ENA first public
attributes.value: 2022-03-31
attributes.tag: ENA last update
attributes.value: 2022-03-31
attributes.tag: External Id
attributes.value: SAMEA13601174
attributes.tag: INSDC center alias
attributes.value: MilliporeSigma
attributes.tag: INSDC center name
attributes.value: MilliporeSigma
attributes.tag: INSDC first public
attributes.value: 2022-03-31T00:18:31Z
attributes.tag: INSDC last update
attributes.value: 2022-03-31T00:18:31Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11557:SCC327 Colon 18-1417N 2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: SCC327
attributes.tag: cell type
attributes.value: epithelial cell of large intestine
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: colorectal carcinoma
attributes.tag: genotype
attributes.value: normal
attributes.tag: growth condition
attributes.value: Matrigel dome
attributes.tag: organism part
attributes.value: colon
attributes.tag: sample name
attributes.value: E-MTAB-11557:SCC327 Colon 18-1417N 2
attributes.tag: sampling site
attributes.value: normal tissue adjacent to neoplasm