title: Smart-seq3
description: Protocols: HEK293FT cells were dissociated using TrypLE. Cells were FACS sorted and lysed into 3 ul of Smart-seq3 lysis buffer supplemented with 0.025 pg of 5'-molecular spike-in and lysate was used for library preparation without nucleic acid extraction. Smart-seq3 library preparations were performed as published: https://www.protocols.io/view/smart-seq3-protocol-bcq4ivywLibrary and with following modifications: The cDNA was cleaned up after reverse transcription. For the pre-amplification PCR different amounts of fwd primer were chosen (0.1, 0.5, 1.0 uM) in presence or absence of template switching oligo.
attributes.tag: ENA first public
attributes.value: 2022-02-16
attributes.tag: ENA last update
attributes.value: 2022-02-16
attributes.tag: External Id
attributes.value: SAMEA8657191
attributes.tag: INSDC center alias
attributes.value: Karolinska Institutet Department of Cell & Molecular Biology
attributes.tag: INSDC center name
attributes.value: Karolinska Institutet Department of Cell & Molecular Biology
attributes.tag: INSDC first public
attributes.value: 2022-02-16T12:28:50Z
attributes.tag: INSDC last update
attributes.value: 2022-02-16T12:28:50Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-10372:Smart-seq3
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: HEK293T
attributes.tag: common name
attributes.value: human
attributes.tag: sample name
attributes.value: E-MTAB-10372:Smart-seq3