title: Infected mice 2 (bacterial RNA enriched)
description: Protocols: This study compared the expression profiles of B. pseudomallei from infected mice to B. pseudomallei cultivated in soil extract media. B. pseudomallei in vivo was created by infecting BALB/c strain mice spleens through the intraperitoneal route and harvesting the spleens on day 5 post infection. Total RNA was obtained from two different sources: 1) mouse organs and 2) bacterial cultures. For the mouse samples, the spleens of infected and non-infected mice were harvested and RNA extracted on day 5 after infection. Bacteria samples were grown in soil extract medium and harvested during the mid-log phase. Total RNA was extracted from mice spleen and cultivated bacteria using the Trizol method, followed by DNA contamination removal and RNA purification using the Qiagen RNAeasy kit. The TruSeq RNA Library Prep Kit was used to prepare an RNAseq sequence library.
attributes.tag: ENA first public
attributes.value: 2022-02-28
attributes.tag: ENA last update
attributes.value: 2022-02-28
attributes.tag: External Id
attributes.value: SAMEA11312961
attributes.tag: INSDC center alias
attributes.value: School of Bioscience and Biotechnology
attributes.tag: INSDC center name
attributes.value: School of Bioscience and Biotechnology
attributes.tag: INSDC first public
attributes.value: 2022-02-28T00:20:13Z
attributes.tag: INSDC last update
attributes.value: 2022-02-28T00:20:13Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11200:Infected mice 2 (bacterial RNA enriched)
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: growth condition
attributes.value: in vivo from mouse spleen
attributes.tag: organism part
attributes.value: whole organism
attributes.tag: protocol
attributes.value: Bacterial RNA enriched
attributes.tag: sample name
attributes.value: E-MTAB-11200:Infected mice 2 (bacterial RNA enriched)
attributes.tag: strain
attributes.value: UKMD286