title: K562 ETO2-ko 144h rep2
description: Protocols: Cell lines were collected at 24h and 144h post doxycycline (DOX) withdrawal or maintained with DOX in media culture. Patient derived xenograft (PDX) cells of AEL patients were obtained from bone marrow and spleen of NSG recipient mice. HEL and K562 ETO2-ko cell lines were maintained with 0.500 ng/mL of doxycycline (DOX). Cells were washed twice with culture medium without DOX before to be maintained without DOX for 24h and 144h DOX removal conditions. RNA was extracted using RNeasy mini Kit (Qiagen) RNA-seq libraries were prepared using SureSelect Automated Strand Specific RNA Library Preparation Kit
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2021-12-30
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2021-12-30
attributes.tag: External Id
attributes.value: SAMEA8569313
attributes.tag: INSDC center alias
attributes.value: INSERM Institut Gustave Roussy
attributes.tag: INSDC center name
attributes.value: INSERM Institut Gustave Roussy
attributes.tag: INSDC first public
attributes.value: 2021-12-30T00:11:42Z
attributes.tag: INSDC last update
attributes.value: 2021-12-30T00:11:42Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-10346:K562 ETO2-ko 144h rep2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: K-562
attributes.tag: common name
attributes.value: human
attributes.tag: compound
attributes.value: Doxycycline
attributes.tag: disease
attributes.value: acute erythroleukemia
attributes.tag: genotype
attributes.value: endogeneous ETO2 homozygous knockout + exogenous Doxycycline-inducible ETO2
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: bone marrow
attributes.tag: phenotype
attributes.value: Doxycycline-induced expression of ETO2 in ETO2 knockout background
attributes.tag: sample name
attributes.value: E-MTAB-10346:K562 ETO2-ko 144h rep2