title: 10xGenomics v2
description: Protocols: HEK293FT cells were dissociated using TrypLE. Cell suspension was supplemented with 3'-molecular spikes and captured in a Chromium controller using the v2 kit as specified by the manufacturer. 10x Genomics libraries were prepared according to the v2 protocol with the following changes: 10 ul of GEM-RT mix was used and the remainder of the GEM-RT mix was discarded to reduce cell numbers. The fragmentation step was extended to 10 minutes. The double-sided bead-cleanup after the fragmentation was changed to a ratio of 0.6:1 and 1:1 respectively. Similarly, the post-ligation cleanup (step 3.4) was increased to 1:1 ratio instead of 0.8:1. The double-sided post-indexing PCR cleanup was performed at 0.6:1 and 1:1 ratios respectively.
attributes.tag: ENA first public
attributes.value: 2022-02-16
attributes.tag: ENA last update
attributes.value: 2022-02-16
attributes.tag: External Id
attributes.value: SAMEA8657192
attributes.tag: INSDC center alias
attributes.value: Karolinska Institutet Department of Cell & Molecular Biology
attributes.tag: INSDC center name
attributes.value: Karolinska Institutet Department of Cell & Molecular Biology
attributes.tag: INSDC first public
attributes.value: 2022-02-16T12:28:50Z
attributes.tag: INSDC last update
attributes.value: 2022-02-16T12:28:50Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-10372:10xGenomics v2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: HEK293T
attributes.tag: common name
attributes.value: human
attributes.tag: sample name
attributes.value: E-MTAB-10372:10xGenomics v2