title: RHE4 TSG6-/- (b) ctrl
description: Protocols: Epidermis reconstructions were stopped after 13th days of growth. RHE were immediately stored at -80°C until total RNA extraction Immortalized N/TERT keratinocytes were grown on a polycarbonate filter and exposed to air-liquid interface during 11 days to produce RHE as described by Frankart et al., 2012 (Exp Dermatol. 2012 Nov;21(11):871-5. doi: 10.1111/exd.12020.) and De Vuyst et al., 2014 (Methods Mol Biol . 2014;1195:191-201. doi: 10.1007/7651_2013_40.). After 11th day of growth, RHE were incubated for 48h with interleukins 4 (50ng/ml) and 13 (50ng/ml) as performed by Do Nascimento-Pedrosa et al., 2016 (Arch Dermatol Res. 2017 Jan;309(1):63-69. doi: 10.1007/s00403-016-1699-7. Epub 2016 Nov 11.). Total RNA of RHE was extracted using ReliaPrep RNA Tissue Miniprep System (Promega) according to manufacturer's protocol Samples were processed using Illumina's TrueSeq mRNA stranded protocol
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2021-12-30
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2021-12-30
attributes.tag: External Id
attributes.value: SAMEA8020527
attributes.tag: INSDC center alias
attributes.value: University of Namur
attributes.tag: INSDC center name
attributes.value: University of Namur
attributes.tag: INSDC first public
attributes.value: 2021-12-30T00:11:39Z
attributes.tag: INSDC last update
attributes.value: 2021-12-30T00:11:39Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-10095:RHE4 TSG6-/- (b) ctrl
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: N/TERT
attributes.tag: cell_type
attributes.value: keratinocyte
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: TSG-6-/- (b)
attributes.tag: growth condition
attributes.value: in vitro reconstructed epidermis
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: skin epidermis
attributes.tag: phenotype
attributes.value: TSG-6 knockout
attributes.tag: sample name
attributes.value: E-MTAB-10095:RHE4 TSG6-/- (b) ctrl
attributes.tag: stimulus
attributes.value: control