title: Delta9
description: Protocols: Liver from control (flox) or Hmgb1 liver knockout mice after a metabolic stress either induced by a 12-week high fat diet consumption or a fasting (6hours)-refeeding (8hours) challenge. Briefly, frozen liver biopsies (100-200 mg) harvested from HMGB1fl/fl and HMGB1ΔHep mice under CD, upon HFD60% or after F/R, were minced and fixed at room temperature in PBS-1% formaldehyde (Sigma-Aldrich, 47608, Saint Louis, MO, USA) for 20 minutes. After sonication, chromatin immunoprecipitation was performed using anti-HMGB1 antibody (Abcam, ab18256, Cambridge, UK). Immunoprecipitated DNA was subjected to library preparation using NEBNext DNA Ultra II kit.
attributes.tag: ENA first public
attributes.value: 2022-03-31
attributes.tag: ENA last update
attributes.value: 2022-03-31
attributes.tag: External Id
attributes.value: SAMEA13410160
attributes.tag: INSDC center alias
attributes.value: INSERM U1301 CNRS U5070 EFS/Univ Paul Sabatier
attributes.tag: INSDC center name
attributes.value: INSERM U1301 CNRS U5070 EFS/Univ Paul Sabatier
attributes.tag: INSDC first public
attributes.value: 2022-03-31T00:18:29Z
attributes.tag: INSDC last update
attributes.value: 2022-03-31T00:18:29Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11543:Delta9
attributes.tag: age
attributes.value: 18 to 20
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: diet
attributes.value: chow
attributes.tag: genotype
attributes.value: HMGB1ΔHep
attributes.tag: organism part
attributes.value: liver
attributes.tag: sample name
attributes.value: E-MTAB-11543:Delta9
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: C57BL/6J