title: HepG2_0
description: Protocols: Cancer cell lines were cultured in 6 cm dish at the concentration of 1×105 cells per dish. After two days, cells were treated with the indicated concentration of lidocaine and cultured for 24 h. Cells were harvested and applied for RNA preparation. Total RNAs from cultured cells were extracted using a FastGene RNA Premium kit (Nippon Genetics Inc., Tokyo, Japan) according to the manufacturer's protocol. RNA quality was evaluated by RNA integrity number (RIN) using Agilent RNA6000 Pico Kit and the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). High quality RNA samples (RIN>7.0) were applied for RNA-seq analysis. One microgram of total RNA was applied for preparing sequence libraries using KAPA mRNA HyperPrep Kit (Kapa Biosystems Inc, Wilmington, MA, USA) following the manufacturer's instructions.
attributes.tag: ENA first public
attributes.value: 2022-02-28
attributes.tag: ENA last update
attributes.value: 2022-02-28
attributes.tag: External Id
attributes.value: SAMEA12289877
attributes.tag: INSDC center alias
attributes.value: Intensive Care Unit, Gunma University Hospital
attributes.tag: INSDC center name
attributes.value: Intensive Care Unit, Gunma University Hospital
attributes.tag: INSDC first public
attributes.value: 2022-02-28T16:29:54Z
attributes.tag: INSDC last update
attributes.value: 2022-02-28T16:29:54Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11320:HepG2_0
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell line
attributes.value: HepG2
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: hepatocellular carcinoma
attributes.tag: organism part
attributes.value: liver
attributes.tag: sample name
attributes.value: E-MTAB-11320:HepG2_0