title: scRNA-seq of purified AT2-like cells derived from mouse embryonic fibroblasts day7
description: MEFs were transduced with 4 foctors (Nkx2-1, Foxa1, Foxa2, Gata6). Ten days after the transduction in organoid culture system, the cells (i.e. unpurified iPULs) were sorted by flow cytometry, followed by subculture every 10 days. The step of subculture including flow cytometry sorting was repeated additinally two times. The sorted cells were further cultured in organoid culture system for 7 days and were analyzed by scRNAseq.
attributes.tag: sample_name
attributes.value: iPUL day7 singel cell RNAseq