title: high complexity 5' molecular spike pool RNA
description: Protocols: 384 replicates of 10 ng synthetic spike-in RNA no further extraction was performed 384 replicates of 10 ng spike-in RNA each were reverse transcribed using the Smart-seq3 protocol at 1/10 volumes and pre-amplified for 8 cycles of PCR. Resulting cDNA was pooled and cleaned using 0.8x 22% PEG beads. Next, we amplified short 100-150 bp amplicons around the TSS using the TSO-specific Smart-seq3 FWD primer containing the Nextera Read 1 overhang and a sequence specific reverse primer for each of the 11 distinct spike-in sequences with a TruSeq Read 2 overhang.
attributes.tag: ENA first public
attributes.value: 2022-02-16
attributes.tag: ENA last update
attributes.value: 2022-02-16
attributes.tag: External Id
attributes.value: SAMEA12998003
attributes.tag: INSDC center alias
attributes.value: Karolinska Institutet Department of Cell & Molecular Biology
attributes.tag: INSDC center name
attributes.value: Karolinska Institutet Department of Cell & Molecular Biology
attributes.tag: INSDC first public
attributes.value: 2022-02-16T12:29:41Z
attributes.tag: INSDC last update
attributes.value: 2022-02-16T12:29:41Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11448:high complexity 5' molecular spike pool RNA
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: sample name
attributes.value: E-MTAB-11448:high complexity 5' molecular spike pool RNA