title: MIR99b-1
description: Protocols: PC3 cells were transfected with NC mimic or miR-99b mimic for 24 h in triplicate and samples were collected. Total RNA was isolated using TRIZOl reagents (Sigma). RNA sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina following manufacturer's instructions (NEB, Ipswich, MA, USA). Briefly, mRNAs were first enriched with Oligo(dT) beads. Enriched mRNAs were fragmented for 15 minutes at 94 °C. First strand and second strand cDNAs were subsequently synthesized. cDNA fragments were end repaired and adenylated at 3'ends, and universal adapters were ligated to cDNA fragments, followed by index addition and library enrichment by limited-cycle PCR. The sequencing libraries were validated on the Agilent TapeStation (Agilent Technologies, Palo Alto, CA, USA), and quantified by using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA) as well as by quantitative PCR (KAPA Biosystems, Wilmington, MA, USA).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2021-12-30
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2021-12-30
attributes.tag: External Id
attributes.value: SAMEA8550116
attributes.tag: INSDC center alias
attributes.value: North Carolina Central University
attributes.tag: INSDC center name
attributes.value: North Carolina Central University
attributes.tag: INSDC first public
attributes.value: 2021-12-30T00:11:43Z
attributes.tag: INSDC last update
attributes.value: 2021-12-30T00:11:43Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-10350:MIR99b-1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: PC-3
attributes.tag: cell_type
attributes.value: epithelial cell
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: prostate carcinoma
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: metastatic site
attributes.value: bone
attributes.tag: organism part
attributes.value: prostate
attributes.tag: sample name
attributes.value: E-MTAB-10350:MIR99b-1