title: Mouse_1
description: Protocols: Established MC38 tumors (~190 mm3) were excised and single cell suspensions were stained and sorted for CD45+TCRβ+T cells. Sorted cells were loaded onto a 10x Chromium Chip using reagents from the Chromium Single Cell 5' Library and Gel Bead Kit (10x Genomics) according to the manufacturer's protocol. Amplified cDNA was used for both 5' RNA-seq library generation and TCR V(D)J targeted enrichmentusing the Chromium Single Cell V(D)J Enrichment Kit for Mouse T Cells (10xGenomics). 5' RNA-seq and TCR V(D)J libraries were prepared following themanufacturer's user guide (10x Genomics). The final libraries were profiled using theBioanalyzer High Sensitivity DNA Kit (Agilent Technologies) and quantified using theKapa Library Quantification Kit (Kapa Biosystems).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2021-12-30
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2021-12-30
attributes.tag: External Id
attributes.value: SAMEA7534170
attributes.tag: INSDC center alias
attributes.value: GENENTECH
attributes.tag: INSDC center name
attributes.value: GENENTECH
attributes.tag: INSDC first public
attributes.value: 2021-12-30T00:11:32Z
attributes.tag: INSDC last update
attributes.value: 2021-12-30T00:11:32Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-9734:Mouse_1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_type
attributes.value: T cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: individual
attributes.value: Mouse_1
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: lymph node
attributes.tag: replicate
attributes.value: 1
attributes.tag: sample name
attributes.value: E-MTAB-9734:Mouse_1
attributes.tag: single cell library construction
attributes.value: 10x TCR enrichment
attributes.tag: strain
attributes.value: C57BL/6
attributes.tag: xenograft
attributes.value: injection with MC38 colon adenocarcinoma cells