title: Ice Core 1 of Site 1
attributes.tag: ENA first public
attributes.value: 2022-03-01
attributes.tag: ENA last update
attributes.value: 2022-03-01
attributes.tag: External Id
attributes.value: SAMEA8547042
attributes.tag: INSDC center alias
attributes.value: iBB-IST
attributes.tag: INSDC center name
attributes.value: iBB-IST
attributes.tag: INSDC first public
attributes.value: 2022-03-01T00:20:41Z
attributes.tag: INSDC last update
attributes.value: 2022-03-01T00:20:41Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: 1I1
attributes.tag: collection date
attributes.value: 2019-02-27
attributes.tag: environment (biome)
attributes.value: Subpolar aquatic biome
attributes.tag: environment (feature)
attributes.value: Hudson Bay Coast
attributes.tag: environment (material)
attributes.value: sea ice
attributes.tag: geographic location (country and/or sea)
attributes.value: Canada
attributes.tag: geographic location (depth)
attributes.value: 0
attributes.tag: geographic location (latitude)
attributes.value: 55.385831
attributes.tag: geographic location (longitude)
attributes.value: -77.61177
attributes.tag: geographic location (region and locality)
attributes.value: Northern Quebec
attributes.tag: investigation type
attributes.value: mimarks-survey
attributes.tag: miscellaneous environmental package
attributes.value: miscellaneous natural or artificial environment
attributes.tag: nucleic acid amplification
attributes.value: PCR
attributes.tag: nucleic acid extraction
attributes.value: Ultraclean Soil DNA Kit (MoBio Laboratories)
attributes.tag: pcr primers
attributes.value: 515F-806R
attributes.tag: project name
attributes.value: Microbial diversity of coastal water and ice in the Hudson Bay (Subarctic)
attributes.tag: sample collection device or method
attributes.value: Kovacs ice corer
attributes.tag: sample name
attributes.value: 1I1
attributes.tag: sample volume or weight for DNA extraction
attributes.value: 1000
attributes.tag: sequencing method
attributes.value: Illumina MiSeq
attributes.tag: target gene
attributes.value: 16S rRNA gene
attributes.tag: target subfragment
attributes.value: V4 on 16S rRNA gene
attributes.tag: temperature
attributes.value: -23