attributes.tag: strain
attributes.value: 1S2D (MHOM/SD/62/1S-CL2D)
attributes.tag: isolate
attributes.value: Leishmania donovani strain 1S2D (MHOM/SD/62/1S-CL2D) was maintained by serial passages in hamsters. Amastigotes were recovered from infected hamster spleen and differentiated into promastigotes in M199 complete medium..
attributes.tag: dev_stage
attributes.value: Promastigote
attributes.tag: sex
attributes.value: not applicable
attributes.tag: tissue
attributes.value: not applicable
attributes.tag: growth_protocol
attributes.value: Leishmania donovani strain 1S2D (MHOM/SD/62/1S-CL2D) was maintained by serial passages in hamsters. Amastigotes were recovered from infected hamster spleen and differentiated into promastigotes in M199 complete medium (M199, 10% FBS, 20 mM HEPES; 100 µM adenine, 2 mM L-glutamine, 10 µg/ml folic acid, 13.7 µM hemin, 4.2 mM NaHCO3, 1xRPMI1640 vitamins, 8 µM 6-biopterin, 100 units penicillin and 100 µg/ml streptomycin, pH 7.4) at 26°C.
attributes.tag: sample_type
attributes.value: Total RNA
attributes.tag: treatment
attributes.value: Hydrazine 45'- aniline 15'
attributes.tag: BioSampleModel
attributes.value: Model organism or animal