title: Rhizopshera from Arabidopsis thaliana Col-0
description: Non sterile seeds of A. thaliana wild-type ecotype Colombia (Col-0) were grown in 7cm diameter pots in soil (LAT-Terra standard topferde, Awita). Growth chambers conditions were 12-hour light and 12-hour dark regime at 18°C during the light phase under fluorescent tubes (6 Lumilux tubes T5, Osram), and 15°C during the dark phase. Rhizosphere (i.e. adherent soil) was scrapped from roots with a sterile scraper. DNA from the rhizosphere (0.25 g adherent soil) was extracted using the PowerSoil DNA Isolation Kit (MO BIO Laboratories, Inc., Carlsbad, CA, USA). DNA extracted from rhizosphere of WT plants (100ng of each sample) was used for the library's construction with the Nextera Flex Library kit. Gene capture by hybridization targeting the 16S and 18S rDNA was performed.