attributes.tag: collection_date
attributes.value: 2019-09-16
attributes.tag: depth
attributes.value: 15-20 cm
attributes.tag: elev
attributes.value: 430 m
attributes.tag: env_broad_scale
attributes.value: woodland area[ENVO:00000109]
attributes.tag: env_local_scale
attributes.value: woodland area[ENVO:00000109]
attributes.tag: geo_loc_name
attributes.value: USA:Wisconsin
attributes.tag: lat_lon
attributes.value: 45.748 N 90.731 W
attributes.tag: annual_precpt
attributes.value: 840 mm
attributes.tag: annual_temp
attributes.value: 5 C
attributes.tag: collection_method
attributes.value: soil core
attributes.tag: cur_land_use
attributes.value: hardwoods (e.g. oak,hickory,elm,aspen)
attributes.tag: cur_vegetation
attributes.value: northern mesic forest, early-to-mid seral
attributes.tag: drainage_class
attributes.value: moderately well
attributes.tag: extreme_event
attributes.value: 16 week lab incubation
attributes.tag: fao_class
attributes.value: Luvisols
attributes.tag: local_class
attributes.value: Freeon silt loam
attributes.tag: local_class_meth
attributes.value: USDA soil classification
attributes.tag: ph
attributes.value: NA
attributes.tag: ph_meth
attributes.value: NA
attributes.tag: pool_dna_extracts
attributes.value: not pooled
attributes.tag: samp_collect_device
attributes.value: Metal core, 1.8 cm dia
attributes.tag: samp_mat_process
attributes.value: 4 C until sieving to 2mm and start of incubation at RT
attributes.tag: samp_size
attributes.value: 50 mg
attributes.tag: samp_vol_we_dna_ext
attributes.value: 50 mg
attributes.tag: sieving
attributes.value: 2mm
attributes.tag: soil_horizon
attributes.value: A horizon
attributes.tag: soil_text_measure
attributes.value: silt loam
attributes.tag: soil_type
attributes.value: Freeon silt loam
attributes.tag: soil_type_meth
attributes.value: USDA soil classification
attributes.tag: store_cond
attributes.value: after incubation, samples for DNA extraction were stored at -80 C for up to 1 month prior to DNA extraction
attributes.tag: Rep
attributes.value: 2
attributes.tag: Blank
attributes.value: N
attributes.tag: TimesMixed
attributes.value: NA
attributes.tag: ExtrBlank
attributes.value: N
attributes.tag: IncubBlank
attributes.value: N
attributes.tag: Initial
attributes.value: N
attributes.tag: WaterBlank
attributes.value: N
attributes.tag: VortexControl
attributes.value: N
attributes.tag: Trt
attributes.value: Meta_A_S
attributes.tag: GroupOrAgit
attributes.value: 1
attributes.tag: Group2
attributes.value: NV
attributes.tag: O2
attributes.value: Anoxic
attributes.tag: Moisture
attributes.value: Saturated
attributes.tag: Meta
attributes.value: Meta
attributes.tag: MetaOrder
attributes.value: 5
attributes.tag: MixingStudy
attributes.value: N
attributes.tag: ConnectivityStudy
attributes.value: Y
attributes.tag: Fwd_Primer_Barcode
attributes.value: ATATACAC
attributes.tag: Rev_Primer_Barcode
attributes.value: CATAGAGA
attributes.tag: seq_meth
attributes.value: Illumina MiSeq 2x250 PE
attributes.tag: env_material
attributes.value: soil
attributes.tag: nucl_acid_ext
attributes.value: Qiagen DNeasy PowerLyzer PowerSoil DNA extraction kit
attributes.tag: target_gene
attributes.value: 16S rRNA
attributes.tag: target_subfragment
attributes.value: V4
attributes.tag: pcr_cond
attributes.value: initial denaturation:98_2; denaturation:98_0.167; annealing:58_0.25; elongation:72_0.167; final elongation:72_2; total cycles: 30
attributes.tag: BioSampleModel
attributes.value: MIMS.me
attributes.tag: BioSampleModel
attributes.value: MIGS/MIMS/MIMARKS.soil