title: ESC_PGK12.1
organism: Mus musculus
attributes.tag: strain
attributes.value: PGK
attributes.tag: isolate
attributes.value: embryonic stem cells
attributes.tag: age
attributes.value: E4
attributes.tag: dev_stage
attributes.value: E4
attributes.tag: sex
attributes.value: female
attributes.tag: tissue
attributes.value: derived from blastocyst
attributes.tag: biomaterial_provider
attributes.value: Heard lab
attributes.tag: cell_line
attributes.value: ESC_PGK
attributes.tag: cell_type
attributes.value: embryonic stem cells
attributes.tag: collected_by
attributes.value: Instittu Curie
attributes.tag: growth_protocol
attributes.value: Cells were maintained on gelatin-coated flasks in serum-containing ES cell medium (DMEM) supplemented with 15% ESC-grade foetal bovine serum, 1000U/ml leukemia inhibitory factor (LIF, Millipore) and 0.1mM B-mercaptoethanol in humidified atmosphere at 37C with 8% CO2.
attributes.tag: isolation_source
attributes.value: Genomic DNA was extracted from cells using the DNeasy blood and tissue kit (Qiagen), following the manufacturers instructions. 1g of genomic DNA was used for preparation of the library.
attributes.tag: sample_type
attributes.value: cell culture
attributes.tag: BioSampleModel
attributes.value: Model organism or animal