title: Ca. Chlorohelix allophototropha enrichment, subculture 21.2c
organism: freshwater metagenome
attributes.tag: isolation_source
attributes.value: Ca. Chlorohelix allophototropha enrichment, subculture 21.2c; a bacterial enrichment culture of water from the upper anoxic zone (3.88 m depth) of a Boreal Shield lake
attributes.tag: collection_date
attributes.value: 2017-09
attributes.tag: geo_loc_name
attributes.value: Canada: near Kenora, Ontario, Lake 227, IISD-Experimental Lakes Area
attributes.tag: lat_lon
attributes.value: 49.69 N 93.69 W
attributes.tag: rel_to_oxygen
attributes.value: anaerobe
attributes.tag: samp_collect_device
attributes.value: Initial inoculum: anoxic water was pumped to the surface through a flushed line using a gear pump. Water was pumped directly into 120 mL glass serum bottles that were allowed to overflow for several seconds and then quickly sealed using rubber stoppers.
attributes.tag: samp_mat_process
attributes.value: Handling of the initial inoculum: water was kept chilled during storage and shipping to the laboratory. Water was then distributed anoxically into glass serum bottles, amended with a ferrous iron-containing freshwater minimal medium, and incubated under light.
attributes.tag: BioSampleModel
attributes.value: Metagenome or environmental