title: Metagenome-assembled genome: SRR19759446_bin.44_metawrap_v1.3_MAG
organism: uncultured Tenericutes bacterium
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-01-17
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-01-17
attributes.tag: External Id
attributes.value: SAMEA112223356
attributes.tag: INSDC center alias
attributes.value: EMG
attributes.tag: INSDC center name
attributes.value: EMG
attributes.tag: INSDC first public
attributes.value: 2023-01-17T12:18:54Z
attributes.tag: INSDC last update
attributes.value: 2023-01-17T12:18:54Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: SRR19759446_bin.44_metawrap_v1.3_MAG
attributes.tag: assembly quality
attributes.value: Many fragments with little to no review of assembly other than reporting of standard assembly statistics
attributes.tag: assembly software
attributes.value: metaSPAdes v3.15.3
attributes.tag: binning parameters
attributes.value: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.
attributes.tag: binning software
attributes.value: metawrap v1.3
attributes.tag: broad-scale environmental context
attributes.value: mouse digestive system
attributes.tag: broker name
attributes.value: EMG broker account, EMBL-EBI
attributes.tag: collection date
attributes.value: not provided
attributes.tag: completeness score
attributes.value: 90.45
attributes.tag: completeness software
attributes.value: CheckM
attributes.tag: contamination score
attributes.value: 0.0
attributes.tag: environmental medium
attributes.value: feces
attributes.tag: geographic location (country and/or sea)
attributes.value: not provided
attributes.tag: geographic location (latitude)
attributes.value: not provided
attributes.tag: geographic location (longitude)
attributes.value: not provided
attributes.tag: investigation type
attributes.value: metagenome-assembled genome
attributes.tag: isolation_source
attributes.value: mouse gut metagenome
attributes.tag: local environmental context
attributes.value: digestive tube
attributes.tag: metagenomic source
attributes.value: mouse gut metagenome
attributes.tag: project name
attributes.value: A growing body of evidence supports the notion that the gut microbiome plays an important role in cancer immunity. However, the underpinning mechanisms remain to be fully elucidated. One attractive hypothesis envisages that among the T cells elicited by the plethora of microbiome proteins a few exist that incidentally recognize neo-epitopes arising from cancer mutations (molecular mimicry (MM) hypothesis). To support MM, the human probiotic Escherichia coli Nissle was engineered with the SIINFEKL epitope (OVA-E.coli Nissle) and orally administered to C57BL6 mice. The treatment with OVA-E.coli Nissle, but not with wild type E. coli Nissle, induced OVA-specific CD8+ T cells and inhibited the growth of tumors in mice challenged with B16F10 melanoma cells expressing OVA. The microbiome shotgun sequencing and the sequencing of TCRs from T cells recovered from both lamina propria and tumors provide evidence that the main mechanism of tumor inhibition is mediated by the elicitation at the intestinal site of cross-reacting T cells, which subsequently reach the tumor environment. Importantly, the administration of Outer Membrane Vesicles (OMVs) from engineered E. coli Nissle, as well as from E. coli BL21(DE3)DompA, carrying cancer-specific T cell epitopes also elicited epitope-specific T cells in the intestine and inhibited tumor growth. Overall, our data strengthen the important role of MM in tumor immunity and assign a novel function of OMVs in host-pathogen interaction. Moreover, our results pave the way to the exploitation of probiotics and OMVs engineered with tumor specific-antigens as personalized mucosal cancer vaccines.
attributes.tag: sample derived from
attributes.value: SAMN29213117
attributes.tag: sample name
attributes.value: SRR19759446_bin.44_metawrap_v1.3_MAG
attributes.tag: sequencing method
attributes.value: Illumina NovaSeq 6000
attributes.tag: taxonomic classification
attributes.value: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Tenericutes;c__;o__;f__;g__;s__
attributes.tag: taxonomic identity marker
attributes.value: multi-marker approach