organism: Przondovirus
attributes.tag: strain
attributes.value: H5
attributes.tag: isolate
attributes.value: H5 was isolated from hospital wastewater using a hypervirulent K. pneumoniae strain ATCC 43816
attributes.tag: collection_date
attributes.value: 2021-08
attributes.tag: env_broad_scale
attributes.value: ENVO:01000781
attributes.tag: env_local_scale
attributes.value: ENVO:00002173
attributes.tag: env_medium
attributes.value: ENVO:00002018
attributes.tag: geo_loc_name
attributes.value: China
attributes.tag: isol_growth_condt
attributes.value: Sewage samples were filtered using 0.22 m pore-size filters (Millipore, USA) to remove most contaminants. Two hundred microliters of the filtrate were added to 0.5 mL of mid-log phase K. pneumoniae culture. After 10 minutes of standing incubation at 37, the mixtures then were tested for plaque formation using the double-layer agar plate method.
attributes.tag: lat_lon
attributes.value: 37.85 N 122.25 E
attributes.tag: propagation
attributes.value: phage:lytic
attributes.tag: BioSampleModel
attributes.value: MIGS.vi
attributes.tag: BioSampleModel
attributes.value: MIGS/MIMS/MIMARKS.wastewater