organism: Homo sapiens
description: Protocols: Briefly, cells were washed with PBS and trypsinized. As a spike-in control, 1.25 millions of mouse NIH/3T3 cells were added to 5 million cells of Par and PacR cells in duplicates. Cells were fixed for 10 minutes with 1% Formaldehyde and suspended in ice-cold Hi-C Lysis Buffer . Streptavidin-C1 beads (Thermo Fisher) were used to capture biotinylated DNA, library preparation was performed using the MicroPlex Library Preparation Kit v2 (Diagenode)
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-01-01
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-01-01
attributes.tag: External Id
attributes.value: SAMEA14381945
attributes.tag: INSDC center alias
attributes.value: Mayo Clinic
attributes.tag: INSDC center name
attributes.value: Mayo Clinic
attributes.tag: INSDC first public
attributes.value: 2023-01-01T00:28:00Z
attributes.tag: INSDC last update
attributes.value: 2023-01-01T00:28:00Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11732:HiChIP Par
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: L3.6pl
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: pancreatic adenocarcinoma
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: pancreatic duct
attributes.tag: phenotype
attributes.value: paclitaxel susceptible
attributes.tag: sample name
attributes.value: E-MTAB-11732:HiChIP Par
attributes.tag: sampling site
attributes.value: celiac lymph node