organism: Homo sapiens
description: Protocols: Cells were removed media and washed once with cold PBS. Next, add 350ul RLT buffer to lyse cells following the protocol of Rneasy Mini Kit (QIAGEN). RNA was extracted from cultured cells under different treatment conditions using the Rneasy Mini Kit (QIAGEN) in accordance with the manufacturer-prescribed protocol. RNA samples were quantified using Qubit 2.0 Fluorometer (Life Technologies, Carlsbad, CA, USA) and RNA integrity was checked using Agilent TapeStation 4200 (Agilent Technologies, Palo Alto, CA, USA). RNA sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina using manufacturer's instructions (NEB, Ipswich, MA, USA). Briefly, mRNAs were first enriched with Oligo(dT) beads. Enriched mRNAs were fragmented for 15 minutes at 94 °C. First strand and second strand cDNAs were subsequently synthesized. cDNA fragments were end repaired and adenylated at 3'ends, and universal adapters were ligated to cDNA fragments, followed by index addition and library enrichment by limited-cycle PCR. The sequencing libraries were validated on the Agilent TapeStation (Agilent Technologies, Palo Alto, CA, USA), and quantified by using Qubit 2.0 Fluorometer (Invitrogen, Carlsbad, CA) as well as by quantitative PCR (KAPA Biosystems, Wilmington, MA, USA).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-12-31
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-12-31
attributes.tag: External Id
attributes.value: SAMEA12548910
attributes.tag: INSDC center alias
attributes.value: MASSACHUSETTS GENERAL HOSPITAL
attributes.tag: INSDC center name
attributes.value: MASSACHUSETTS GENERAL HOSPITAL
attributes.tag: INSDC first public
attributes.value: 2022-12-31T00:57:45Z
attributes.tag: INSDC last update
attributes.value: 2022-12-31T00:57:45Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11324:17
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: ICC10-6
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: intrahepatic cholangiocarcinoma
attributes.tag: genotype
attributes.value: FGFR2-PHGDH
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: bile duct
attributes.tag: sample name
attributes.value: E-MTAB-11324:17