organism: Homo sapiens
description: Protocols: CD19+ B cells from tumor-draining lymph nodes (S), non-draining lymph nodes (N), and peripheral blood (P) were positively isolated using a Dynabeads Pan B cell kit (Thermo scientific # 11143D). Isolated B cells were washed and counted. Nucleospin XS kit (Macherey-Nagel # 740902.50) was used for total RNA isolation according to the manufacturer's instructions. 1 – 10 ng of total RNA was used for the generation of IgM/IgG heavy and light chain libraries of V(D)J variable regions of BCR transcripts employing the SMARTer Human BCR IgG IgM H/K/L Profiling Kit (Takara Bio, #634466).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-01-01
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-01-01
attributes.tag: External Id
attributes.value: SAMEA110177359
attributes.tag: INSDC center alias
attributes.value: Laboratory of Molecular Infection Medicine Sweden (MIMS), Department of molecular biology, Umea university
attributes.tag: INSDC center name
attributes.value: Laboratory of Molecular Infection Medicine Sweden (MIMS), Department of molecular biology, Umea university
attributes.tag: INSDC first public
attributes.value: 2023-01-01T00:28:12Z
attributes.tag: INSDC last update
attributes.value: 2023-01-01T00:28:12Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11862:S3
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_type
attributes.value: B cell
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: prostate cancer
attributes.tag: disease staging
attributes.value: T3a
attributes.tag: gleason score
attributes.value: gleason score 9
attributes.tag: immunophenotype
attributes.value: CD19 positive
attributes.tag: individual
attributes.value: 3
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: lymph node
attributes.tag: prostate specific antigen score
attributes.value: prostate specific antigen score 8.6
attributes.tag: sample name
attributes.value: E-MTAB-11862:S3
attributes.tag: sampling site
attributes.value: tumor draining