organism: Danio rerio
description: Protocols: At 72 hpf embryos were sacrificed by cold exposure, incubating the multiwell plates on crushed ice. Embryos were transferred to eppendorfs, medium was removed and they were frozen in N2(l). Tissue was stored at -80ºC until use. RNA was obtained using PureLinkTM RNA Mini Kit (Ambion Fisher Scientific) following the manufacturer´s instructions. In-column DNase I digestion (Invitrogen Fisher Scientific) was performed to remove the traces of gDNA. RNA concentration was initially determined with a NanoDrop 2000C spectrophotometer (Thermo Scientific), the purity of the sample was established according to the ratio of absorbances measured at 260/280 nm, and RNA integrity was check with a Bioanalyzer RNA (Agilent). Ribosomal RNA was depleted using RiboMinus™ RNA was fragmented, reverse transcrided using random hexamers, 3' and 5' adapters were attached and library was amplified by PCR
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-12-31
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-12-31
attributes.tag: External Id
attributes.value: SAMEA12512746
attributes.tag: INSDC center alias
attributes.value: Dept. Biochemistry and Molecular Biology. Faculty of Medicine. Instituto de Neurociencias de Castilla y Leon (INCyL). University of Salamanca, Spain.
attributes.tag: INSDC center name
attributes.value: Dept. Biochemistry and Molecular Biology. Faculty of Medicine. Instituto de Neurociencias de Castilla y Leon (INCyL). University of Salamanca, Spain.
attributes.tag: INSDC first public
attributes.value: 2022-12-31T00:57:45Z
attributes.tag: INSDC last update
attributes.value: 2022-12-31T00:57:45Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11346:Cocaine 3
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: zebrafish
attributes.tag: developmental stage
attributes.value: embryo
attributes.tag: developmental stage
attributes.value: 72
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: whole organism
attributes.tag: sample name
attributes.value: E-MTAB-11346:Cocaine 3
attributes.tag: strain
attributes.value: AB