organism: Homo sapiens
description: Protocols: SiHa cells were cultured in a CO2 incubator using DMEM supplemented with 10% FBS. RNA was extracted using the TRIzol method. The mRNA was subjected to first strand synthesis, followed by second strand synthesis. The double stranded cDNA was purified using HighPrep magnetic beads. The purified cDNA was end-repaired, adenylated and ligated to Illumina multiplex barcode adapters as per NEBNext® Ultra™ Directional RNA Library Prep Kit protocol.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-12-31
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-12-31
attributes.tag: External Id
attributes.value: SAMEA111350867
attributes.tag: INSDC center alias
attributes.value: St. John's Medical College Hospital, Bangalore
attributes.tag: INSDC center name
attributes.value: St. John's Medical College Hospital, Bangalore
attributes.tag: INSDC first public
attributes.value: 2022-12-31T00:58:37Z
attributes.tag: INSDC last update
attributes.value: 2022-12-31T00:58:37Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12162:SiHa-R 2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: SiHa
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: squamous cell carcinoma
attributes.tag: genotype
attributes.value: pcDNA3-RhoC overexpression
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: uterine cervix
attributes.tag: sample name
attributes.value: E-MTAB-12162:SiHa-R 2