organism: Homo sapiens
description: Protocols: A549 and H358 cells were used and treated for 5 days. Cells were collected and homogenized in Trizol Reagent (Lab. Prof. Feliciello); total RNA extraction was performed with an RNA Clean and Concentrator Kit (Zymo Research Corp., Irvine, CA, USA), following the manufacturer's instructions RNA purity and integrity were assessed with a Nanodrop 2000c spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA) and a 4200 TapeStation instrument (Agilent Technologies, Santa Clara, CA, USA), respectively. For RNA purity, an A260/280 ratio of 2.0 and a A260/230 ratio of 2.0-2.2 were considered acceptable; for RNA integrity, an RNA Integrity Number (RIN) of 9.0-10.0 has been obtained for all samples, indicating the absence of degradation and high integrity of RNA samples. For a precise estimation of the RNA concentration, a Qubit 2.0 fluorometer assay (Thermo Fisher Scientific, Waltham, MA, USA) has been employed
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-12-31
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-12-31
attributes.tag: External Id
attributes.value: SAMEA12009169
attributes.tag: INSDC center alias
attributes.value: Laboratory of Molecular Medicine and Genomics
attributes.tag: INSDC center name
attributes.value: Laboratory of Molecular Medicine and Genomics
attributes.tag: INSDC first public
attributes.value: 2022-12-31T00:57:39Z
attributes.tag: INSDC last update
attributes.value: 2022-12-31T00:57:39Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11253:Sample 2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: A549
attributes.tag: cell_type
attributes.value: lung cancer cell
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: lung cancer
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: lung
attributes.tag: sample name
attributes.value: E-MTAB-11253:Sample 2