organism: Homo sapiens
description: Protocols: SH-SY5Ycells from ATCC. Gimen from DSMZ. For sequencing: Triplicates were pooled in equal amounts into one sample. All cell lines were cultured in Dulbecco's Modified Eagle Medium (DMEM (high Glucose plus Glutamine and Pyruvate), Invitrogen) supplemented with 10% fetal bovine serum (FBS) and 1% Penicillin/ Streptomycin at 37°C with 5% CO2. Day0.5: Change to 0.5%FBS +DMSO or ISX (20µM)Day2.5 Harvest Macherey-Nagel NucleoSpin RNA Isolation Kit, RNA Isolation according to manufactures protocol. Dnase Treatment according to manufacture's protocol. Illumina TruSeq total RNA v2
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-12-31
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-12-31
attributes.tag: External Id
attributes.value: SAMEA110278103
attributes.tag: INSDC center alias
attributes.value: Philipps University Marburg, Bioinformatics Core Facility, Center for Tumor- and Immune Biology,
attributes.tag: INSDC center name
attributes.value: Philipps University Marburg, Bioinformatics Core Facility, Center for Tumor- and Immune Biology,
attributes.tag: INSDC first public
attributes.value: 2022-12-31T00:58:25Z
attributes.tag: INSDC last update
attributes.value: 2022-12-31T00:58:25Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11915:MA044
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: GI-ME-N
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: juvenile stage
attributes.tag: disease
attributes.value: neuroblastoma
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: brain
attributes.tag: sample name
attributes.value: E-MTAB-11915:MA044