organism: Zea mays
description: corn was planted with four individual weeds (canola) surrounding a corn seed in the center of an 8 pot. The weeds were planted outside conetainers so that there was direct root-to-root contact between the corn and the canola. To block the above-ground weed-generated signals, an opaque plastic cone-shaped barrier was placed, point down, over the corn seedling to prevent any weed-generated light quality (increased far-red light due to reflection from the canola) signal from impacting the corn seedling. This cone was enlarged as the corn and canola grew so that any above-ground light quality signal was blocked until after the corn had reached the V8 stage of growth. The plants were grown under greenhouse conditions with diffuse light and an 18 Hr photoperiod at about 25 C for 3 months (until the plants were at the V12 stage of growth). Plants were watered daily and fertilized weekly by additional watering with 20:20:20. Plant root (including roughly equal amount by weight of support roots and horizontal roots) and leaf tips (the distal 10 cm of the top-most fully expanded leaf) were harvested separately into liquid nitrogen for future RNA extractions.
attributes.tag: isolate
attributes.value: 84 day
attributes.tag: cultivar
attributes.value: commercial hybrid
attributes.tag: ecotype
attributes.value: NA
attributes.tag: age
attributes.value: 3 months
attributes.tag: dev_stage
attributes.value: V12
attributes.tag: collection_date
attributes.value: 2022-06-22
attributes.tag: geo_loc_name
attributes.value: USA: Fargo, ND
attributes.tag: tissue
attributes.value: mix or lateral and support roots
attributes.tag: biomaterial_provider
attributes.value: David Horvath
attributes.tag: collected_by
attributes.value: Laura Kelley
attributes.tag: temp
attributes.value: 20-25C
attributes.tag: treatment
attributes.value: weedy with only above-ground barriers
attributes.tag: BioSampleModel
attributes.value: Plant