organism: Plasmodium falciparum 3D7
attributes.tag: source_name
attributes.value: 3D7
attributes.tag: Stage
attributes.value: Ring (4hpi)
attributes.tag: chip antibody
attributes.value: H3K4me
attributes.tag: isolation
attributes.value: Cultures were synchronized to the ring stage by two sorbitol treatments then cultured for 25 hours in the presence of heparin (20 U/ml) to prevent reinvasion of erythrocytes. Once the majority of parasites had developed to the late schizont stage (fully segmented schizonts as assessed by Giemsa stain), the culture was centrifuged at 250 × g for 5 min. at RT to pellet the erythrocytes. The supernatant containing heparin was removed, and the erythrocytes resuspended in complete culture medium (5% hematocrit). The culture was incubated under normal growth conditions (37°C in an atmosphere of 5% O2, 5% CO2, and 90% N2) for 4 hours to allow reinvasion. Following the reinvasion window, mature parasites were removed from the culture by treatment with 5% sorbitol. To ensure complete removal of mature parasites, remaining trophozoite- and schizont-infected erythrocytes were removed by magnetic separation using a MidiMACS separator. Isolated ring stage (4 hpi) parasites were released from erythrocytes by saponin treatment.
attributes.tag: geo_loc_name
attributes.value: missing
attributes.tag: collection_date
attributes.value: missing
attributes.tag: isolate
attributes.value: 3D7