organism: Severe acute respiratory syndrome coronavirus 2
description: Severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2), isolate hCoV-19/USA/MD-HP20874/2021 was isolated from a nasal swab in Maryland, USA, on November 27, 2021. NR-56462 lot 70049427 was produced by infecting Homo sapiens lung adenocarcinoma epithelial cells (Calu-3; ATCC® HTB-55TM) with the deposited material and incubating in Eagle's Minimum Essential Medium (ATCC® 30-2003TM) supplemented with 2% fetal bovine serum (ATCC® 30-2020TM) for 7 days at 37°C with 5% CO2. The cells and supernatant were spin-clarified at 1500 × g for 10 minutes at 4°C.
attributes.tag: strain
attributes.value: Omicron Lineage BA.5
attributes.tag: isolate
attributes.value: hCoV-19/USA/MD-HP20874/2021
attributes.tag: collected_by
attributes.value: BIOQUAL
attributes.tag: collection_date
attributes.value: 2021-11
attributes.tag: geo_loc_name
attributes.value: USA: Maryland
attributes.tag: host
attributes.value: Homo sapiens
attributes.tag: host_disease
attributes.value: COVID-19
attributes.tag: isolation_source
attributes.value: not applicable
attributes.tag: lat_lon
attributes.value: not applicable
attributes.tag: culture_collection
attributes.value: not provided
attributes.tag: Replicate
attributes.value: Biological replicate 2
attributes.tag: BioSampleModel
attributes.value: Pathogen.cl