organism: Homo sapiens
description: Protocols: subconfluent cells harvested with Trizol 769-P cells infected with lentivirus containing CAS9 and specific gRNA; puromycin selection; then harvested. phenol/chloroform extraction, isopropanol precipitation poly A purfication, cDNA preparation
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: disease
attributes.value: clear cell renal carcinoma
attributes.tag: organism
attributes.value: Homo sapiens
attributes.tag: organism
attributes.value: Homo sapiens
attributes.tag: cell_line
attributes.value: 769-P
attributes.tag: collection date
attributes.value: not collected
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: common name
attributes.value: human
attributes.tag: cell_type
attributes.value: CMKLR1 knockout cells
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected