organism: Mus musculus
description: Protocols: The floxed mutant mice possess loxP sites flanking exon 4 of the transforming growth factor, beta receptor II (TGFbR2) targeted gene. These mutant mice were bred to mice that express Cre recombinase under the control of the endogenous Lyz2 promoter/enhancer elements so that myeloid cell lineage (monocytes, mature macrophages and granulocytes) do not express the TGFbR2 gene. For single cell analysis NPLC were isolated with the Liver Dissociation Kit from Miltenyi Biotec according to the manufacture instructions. The cells were blocked by Fc-Block (1:200, True stain FcX Plus Biolegend) for 10 minutes on ice, than incubated on ice with Zombie (1:1000, Biolegend), CD45 (1:100, Biolegend) and F4/80 (1:100, Biolegend) in PBS with 2 mM EDTA for 30 minutes. The cells were washed and vital, CD45 positive, F4/80 positive cells were sorted using a BD FACS Aria III SORP Cell sorter (BD Biosciences) and a 100 μm nozzle. Single cell processing was carried out on the 10X Chromium Controller system utilizing the Chromium Single-Cell 3'NextGEM Reagent-Kit-v3.1 (10X Genomics, Pleasanton, CA) aiming for an average recovery of 10,000 cells per sample Chromium Single-Cell 3'NextGEM Reagent-Kit-v3.1 according to protocol
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-12-31
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-12-31
attributes.tag: External Id
attributes.value: SAMEA14383524
attributes.tag: INSDC center alias
attributes.value: Heinrich-Heine-University Duesseldorf Biologisch-Medizinisches Forschungszentrum (BMFZ) Genomics & Transcriptomics Labor (GTL)
attributes.tag: INSDC center name
attributes.value: Heinrich-Heine-University Duesseldorf Biologisch-Medizinisches Forschungszentrum (BMFZ) Genomics & Transcriptomics Labor (GTL)
attributes.tag: INSDC first public
attributes.value: 2022-12-31T00:58:19Z
attributes.tag: INSDC last update
attributes.value: 2022-12-31T00:58:19Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11727:335-3
attributes.tag: age
attributes.value: 8
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_type
attributes.value: macrophage
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: Tgfbr2tm1Roes/RoesH
attributes.tag: immunophenotype
attributes.value: CD45 positive, F4/80 positive
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: liver
attributes.tag: sample name
attributes.value: E-MTAB-11727:335-3
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: C57BL/6J; 129