organism: Homo sapiens
description: Protocols: Tumor and T cells from splenic lymphocytes from ten HCLc patients were purified using FACS. Splenocytes were stained with CD3-PE (clone: UCHT1, Biolegend), 20 μg/ml CD11c-FITC (clone: Bu15, Biolegend), 5 μg/ml CD103-PE/Cy7 (clone: Ber-ACT8, Biolegend), and 2.5 μg/ml CD19-APC (clone: HIB19, Biolegend). Tumor cells were isolated based on the co-expression of CD19, CD11c and CD103; and T cells based on CD3 expression. Data was analyzed using FACS Diva (BD) and FlowJo (TreeStar). Genomic DNA from purified cell populations were extracted using QIAamp DNA mini kit (Qiagen, Manchester, UK), according to the manufacturer's instructions. DNA from tumor and T cells were used for library preparation using NEBNext UltraTM DNA Library Prep kit (New England Biolabs, Hitchin, UK), according to manufacturer's instructions.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-01-01
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-01-01
attributes.tag: External Id
attributes.value: SAMEA110586274
attributes.tag: INSDC center alias
attributes.value: The University of Texas MD Anderson Cancer Center Department of Lymphoma & Myeloma
attributes.tag: INSDC center name
attributes.value: The University of Texas MD Anderson Cancer Center Department of Lymphoma & Myeloma
attributes.tag: INSDC first public
attributes.value: 2023-01-01T00:28:23Z
attributes.tag: INSDC last update
attributes.value: 2023-01-01T00:28:23Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12046:HC10
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_type
attributes.value: T cell
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: hairy cell leukemia
attributes.tag: individual
attributes.value: HCL10
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: spleen
attributes.tag: sample name
attributes.value: E-MTAB-12046:HC10
attributes.tag: sampling site
attributes.value: neoplasm