organism: Mus musculus
description: Protocols: Adult mice (3 strains - Adarb1 -/- C57BL/6J, Gria2 R/R C57BL/6J, C57BL/6J) were maintained under a 12/12-h light–dark regime for 2 weeks before the experiments. After this period of time, the animals were released into constant darkness at the time of dark-to-light transition(designated as circadian time 0; CT0). During the first cycle in darkness, the animals were sacrificed by rapid decapitation at CT5 and CT 18. Acute rat brain tissue was dissected. hypothalamic tissue containing SCNs were punched out under visual control and frozen in dry ice. Total RNA was extracted from frozen samples using the Qiagen RNaesy kit according to manufacturer´s protocol. The quantity and quality of isolated RNA was measured using NanoDrop ND-2000 (NanoDrop Technologies) and analysed by Agilent 2100 Bioanalyser (Agilent Technologies). RNA integrity number, which is regarded as criteria for high-quality total RNA, ranged between 6.5 and 9.8. For each sample, 5 ng of total RNA was used as input material for libraries preparation by following the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian user manual (Takara Bio USA, Inc).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-12-31
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-12-31
attributes.tag: External Id
attributes.value: SAMEA12813644
attributes.tag: INSDC center alias
attributes.value: Institute of Physiology, Czech Academy of Sciences BIOCEV
attributes.tag: INSDC center name
attributes.value: Institute of Physiology, Czech Academy of Sciences BIOCEV
attributes.tag: INSDC first public
attributes.value: 2022-12-31T00:57:47Z
attributes.tag: INSDC last update
attributes.value: 2022-12-31T00:57:47Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11408:2_B6_10D_S34
attributes.tag: age
attributes.value: 3
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: wildtype genotype
attributes.tag: individual
attributes.value: M1
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: hypothalamus
attributes.tag: sample name
attributes.value: E-MTAB-11408:2_B6_10D_S34
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: C57BL/6J
attributes.tag: time
attributes.value: CT17