organism: Bos taurus
description: Protocols: To identify expression quantitative trait loci (eQTL) in Hanwoo cattle, 32 animals were provided by the Hanwoo cattle Research institute, National Institute of Animal Science, South Korea. For RNA-sequencing, tissue samples, such as Abdominal Fat (ABF), Back Fat (BFT) and Kidney Fat (KIF) were collected from the castrated Hanwoo cattle after slaughtering at 10~26 months of age. Ethics approval was obtained from the National Institute of Animal Science (approval no: NIAS20201979). Total RNA was extracted from the trachea with TRIzol Reagent (Invitrogen, Carlsbad, CA, USA) following the manufacturer's protocol. RNA concentration was checked using a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, USA). Individual libraries were generated using Illumina TruSeq™ RNA Sample Preparation Kit (Illumina, San Diego, CA, USA).
attributes.tag: phenotype
attributes.value: case
attributes.tag: strain
attributes.value: Hanwoo cattle (Korean native cattle)
attributes.tag: organism
attributes.value: Bos taurus
attributes.tag: organism
attributes.value: Bos taurus
attributes.tag: collection date
attributes.value: not collected
attributes.tag: sex
attributes.value: castrated male
attributes.tag: scientific_name
attributes.value: Bos taurus
attributes.tag: common name
attributes.value: cattle
attributes.tag: organism part
attributes.value: Abdominal fat; Back fat; Kidney fat
attributes.tag: body weight
attributes.value: 602
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: age
attributes.value: 26
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-10-16