organism: Mus musculus
description: Protocols: Cells were harvested by trypsinization, washed with PBS and mixed in RNA lysis buffer (Zymo Research). Cardiac tissue was lysed in 600 µL of RNA lysis buffer (Zymo) and homogenized using TissueLyserII (Qiagen). Homogenate was spun down for 2 min at 15000 g and the supernatant was processed according to the manufacturer's instructions. Zymo Quick-RNA MicroPrep isolation kit was used to extract total RNA. 100% ethanol was added to the lysates and samples were than processed on the columns. DNA contamination was removed by DNase I digestion. RNA was washed and eluted in 20 µl of water. RNA concentration and purity were assessed with Nanodrop 1000 (Thermo Fisher Scientific) RNA-seq library preparation workflow Figure 2.2.1 Strand-specific RNA sequencing with polyA selection.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-10-27
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-10-27
attributes.tag: External Id
attributes.value: SAMEA111479029
attributes.tag: INSDC center alias
attributes.value: Center of Experimental Rheumatology, Department of Rheumatology, University Hospital Zurich, University of Zurich
attributes.tag: INSDC center name
attributes.value: Center of Experimental Rheumatology, Department of Rheumatology, University Hospital Zurich, University of Zurich
attributes.tag: INSDC first public
attributes.value: 2022-10-27T00:17:18Z
attributes.tag: INSDC last update
attributes.value: 2022-10-27T00:17:18Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12271:Sample 7
attributes.tag: age
attributes.value: 16
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: rheumatic disease
attributes.tag: genotype
attributes.value: Fosl-2
attributes.tag: organism part
attributes.value: heart
attributes.tag: sample name
attributes.value: E-MTAB-12271:Sample 7
attributes.tag: strain
attributes.value: BTBR mouse