organism: Homo sapiens
description: Protocols: Blood (70 ml) from RA patients and control group were collected, and peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll density and dextran sedimentation. In both studied groups, monocytes were isolated from PBMCs by depletion of non-monocytes cells using an indirect magnetic labeling system. Classical monocytes were indirectly isolated using the CD16 MicroBeads human kit (Miltenyi Biotec®, Germany). Classical monocytes RNA was isolated using the RNeasy Plus Mini (Qiagen®), and RNA integrity number (RIN ≥ 7) was evaluated by the ScreenTape method using Bioanalyser 2100 (Agilent®). RNA library was constructed using the Quant-seq® 30 mRNA-Seq Library Prep Kit (Lexogen®).
attributes.tag: disease
attributes.value: Polyarticular juvenile idiopathic arthritis
attributes.tag: individual
attributes.value: AIJ-12
attributes.tag: organism
attributes.value: Homo sapiens
attributes.tag: organism
attributes.value: Homo sapiens
attributes.tag: collection date
attributes.value: not collected
attributes.tag: sex
attributes.value: female
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: common name
attributes.value: human
attributes.tag: organism part
attributes.value: blood
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: cell_type
attributes.value: CD14-positive, CD16-negative classical monocyte
attributes.tag: age
attributes.value: 33
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected