organism: Mus musculus
description: Protocols: Mice were subject to a short-term fast of 4 hours before anesthesia with 2.5% isoflurane. A liver biopsy was taken from the right medial lobe and stored in RNAlater a -20 degree C until analysis. Raw sequencing data were processed using CASAVA v. 1.8 conversion software (Illumina). Total RNA was purified by an AllPrep DNA/RNA/ protein extraction kit (QIAgen) according to manufacturer's instructions and qualified using a 2100 Bioanalyzer instrument (Agilent Genomics). RNA-sequencing was done using TruSeq Stranded Total RNA Library Prep Kit (Illumina) according to the manufacturer's protocol: Ribosomal RNA was depleted using TruSeq Standed RNA Ribominus Gold (Illumina), and cDNA libraries were purified using AMPure XP Beads (Beckman). Following end-repair and poly(A)-tailing, adaptor ligation was performed with Truseq RNA single indexes set A/B (Illumina).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-10-12
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-10-20
attributes.tag: External Id
attributes.value: SAMEA110585942
attributes.tag: INSDC center alias
attributes.value: Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen
attributes.tag: INSDC center name
attributes.value: Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen
attributes.tag: INSDC first public
attributes.value: 2022-10-12T09:00:52Z
attributes.tag: INSDC last update
attributes.value: 2022-10-20T16:34:43Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12060:GR605
attributes.tag: age
attributes.value: 10
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: Gcgr knockout
attributes.tag: organism part
attributes.value: liver
attributes.tag: sample name
attributes.value: E-MTAB-12060:GR605
attributes.tag: sex
attributes.value: male