organism: Homo sapiens
description: Protocols: IMR32 cell lines were ordered from ATCC cell lines were cultured in Dulbecco's Modified Eagle Medium (DMEM (high Glucose plus Glutamine and Pyruvate), Invitrogen) supplemented with 0.5% fetal bovine serum (FBS) and 1% Penicillin/ Streptomycin at 37°C with 5% CO2. ISX-9 was applied for 48 h. Attached cells were washed and treated with DNaseI (200 U/ml in 250mM MgCl2/50mM CaCl2) for 30 min at 37°C. Subsequently, cells were washed with PBS, trypsinized and 100.000 cells of each condition were sent to Active Motif for ATACseq custom service. Active Motif ATACSeq custom service.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-12-31
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-12-31
attributes.tag: External Id
attributes.value: SAMEA14089702
attributes.tag: INSDC center alias
attributes.value: Philipps University Marburg, Bioinformatics Core Facility, Center for Tumor- and Immune Biology
attributes.tag: INSDC center name
attributes.value: Philipps University Marburg, Bioinformatics Core Facility, Center for Tumor- and Immune Biology
attributes.tag: INSDC first public
attributes.value: 2022-12-31T00:58:15Z
attributes.tag: INSDC last update
attributes.value: 2022-12-31T00:58:15Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11633:ISX-1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: IMR-32
attributes.tag: cell_type
attributes.value: neuroblast (sensu Vertebrata)
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: infant
attributes.tag: disease
attributes.value: neuroblastoma
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: brain
attributes.tag: sample name
attributes.value: E-MTAB-11633:ISX-1