organism: Homo sapiens
description: Protocols: Cryopreserved primary human hepatocytes were purchased from Lonza. Primary human hepatocytes were plated for 6 hours and incubated for 66 hours with either vehicle (DMSO) or a 5-probe cocktail (Sanofi-Aventis). Hepatic steatosis was modeled by incubating the cells with free fatty acid (FFA) corresponding to 200 µM mixture of a 2:1 ratio of oleic acid to palmitic acid for a total of72 hours. Four different conditions have thereby been studied: i) Vehicle (DMSO 0.5% v/v); ii) Cocktail (66h five-drug cocktail incubation); iii) FFA (2:1 oleic:palmitic acid) and iv) FFA+Cocktail (Combination of FFA incubation and 5-drug cocktail). The components of the cocktail were dissolved in DMSO to a final concentration of: 50 mM of Caffeine; 30 mM of Metoprolol;n25 mM of Omeprazole; 20 mM of S-Warfarin and 5.82 mM of Midazolam Single-cell encapsulation and lysis in oil droplets was performed following the Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 User Guide Libraries were prepared following the Chromium Next GEM Single Cell 3ʹ Reagent Kits v3.1 User Guide.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-12-31
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-12-31
attributes.tag: External Id
attributes.value: SAMEA13409659
attributes.tag: INSDC center alias
attributes.value: Helmholtz Pioneer Campus
attributes.tag: INSDC center name
attributes.value: Helmholtz Pioneer Campus
attributes.tag: INSDC first public
attributes.value: 2022-12-31T00:58:10Z
attributes.tag: INSDC last update
attributes.value: 2022-12-31T00:58:10Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11530:MUC14331_3
attributes.tag: age
attributes.value: 18
attributes.tag: batch
attributes.value: 1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_type
attributes.value: hepatocyte
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: individual
attributes.value: HUM4152
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: liver
attributes.tag: sample name
attributes.value: E-MTAB-11530:MUC14331_3
attributes.tag: sex
attributes.value: male