organism: Arabidopsis thaliana
description: Protocols: At the indicated time points, seedlings were removed from liquid media in wells, lightly blotted dry, placed into 2mL centrifuge tubes containing 3 glass beads, and flash-frozen in liquid nitrogen. Two wells, one from each of two plates, were combined per sample, for a total of four plants (2 seedlings per well). Arabidopsis seeds were germinated on 1/2 strength MS 1% sucrose 0.8% phytoagar plates after 3-5 days stratification on plates. 4-day-old seedlings were moved in sterile conditions to liquid 1/2 MS 1% sucrose in 24-well plates, 2 seedlings and 1mL media per well. Seedlings were allowed to grow for 9 more days. When seedlings were 13 days old, MS media was decanted, and 600 uL fresh media added to each well. Plates were left overnight. When seedlings were 14 days old, 400 uL of 2.5x elf18 solution (2.5 uM in 1/2 MS, 1% sucrose) or mock (1/2 MS, 1% sucrose) was added to each well, for a final concentration of 1 uM in 1 mL or mock in 1 mL. Seedlings remained in elicitor solution for 10,30, or 90 minutes before harvesting. Total RNA was extracted via a standard TRIzol extraction. Briefly, frozen tissue was ground, TRIzol solution added to powder, and cell debris pelleted by centrifugation. The aqueous phase of the TRIzol was then removed and RNA precipitated via an isopropanol precipitation followed by ethanol wash. DNA was removed using Ambion TURBO DNAse, and RNA purified on a column. Nucleic acid libraries were prepared by BGI, using rolling circle amplification.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-12-31
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-12-31
attributes.tag: External Id
attributes.value: SAMEA8241080
attributes.tag: INSDC center alias
attributes.value: The University of Zurich
attributes.tag: INSDC center name
attributes.value: The University of Zurich
attributes.tag: INSDC first public
attributes.value: 2022-12-31T00:56:37Z
attributes.tag: INSDC last update
attributes.value: 2022-12-31T00:56:37Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-10210:Sample 13
attributes.tag: age
attributes.value: 2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: thale cress
attributes.tag: developmental stage
attributes.value: seedling development stage
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: whole plant
attributes.tag: sample name
attributes.value: E-MTAB-10210:Sample 13
attributes.tag: stimulus
attributes.value: none
attributes.tag: ecotype
attributes.value: Col-0