organism: Fragaria vesca
description: Protocols: All Fragaria vesca samples were collected from the crown (rhizome) of the plant, flash-frozen in liquid nitrogen and stored at -80 °C until RNA isolation. Sampling was performed at 48 hours after i) wounded and inoculated with Phytophthora cactorum, ii) wounded and inoculated with water, and iii) untreated control. Phytophthora cactorum was grown in V8 agar for two weeks at room temperature (20 °C) and zoospore suspension were made in autoclaved pond water. Zoospores were observed under a light microscope and counted using a hemocytometer-type grid KOVA® Glasstic Slide (Hycor, USA). Plants were gently wounded in the crown with a sterilised scalpel and inoculated with 2 mL of Phytophthora cactorum zoospore suspension (2 × 105 spores/mL) or water. Frozen crown tissues were ground using liquid nitrogen and total RNA was isolated using the SpectrumTM Plant Total RNA Kit (Sigma-Aldrich, USA) according to the manufacturer's instruction. RNA was dissolved in elution buffer provided with the kit and diluted in nuclease-free water. RNA quantity and quality was measured with the Nanodrop 2000 Spectrophotometer (Thermo Scientific, USA) and the Agilent RNA 6000 Nano kit with Agilent 2100 Bioanalyzer (Agilent Technology, USA). Libraries were prepared with the TruSeqTM stranded total RNA library prep kit (Illumina, USA).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-01-01
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-01-01
attributes.tag: External Id
attributes.value: SAMEA111328954
attributes.tag: INSDC center alias
attributes.value: Department of Molecular Plant Biology, Division for Biotechnology and Plant Health, Norwegian Institute of Bioeconomy Research (NIBIO)
attributes.tag: INSDC center name
attributes.value: Department of Molecular Plant Biology, Division for Biotechnology and Plant Health, Norwegian Institute of Bioeconomy Research (NIBIO)
attributes.tag: INSDC first public
attributes.value: 2023-01-01T00:28:36Z
attributes.tag: INSDC last update
attributes.value: 2023-01-01T00:28:36Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12152:NCGR1603_wounded_inoculated_R3
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: wild strawberry
attributes.tag: cultivar
attributes.value: NCGR1603
attributes.tag: developmental stage
attributes.value: sporophyte vegetative stage
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: individual
attributes.value: mixed pool of 4 plants
attributes.tag: infect
attributes.value: Phytophthora cactorum
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: rhizome
attributes.tag: phenotype
attributes.value: Phytophthora cactorum resistant
attributes.tag: sample name
attributes.value: E-MTAB-12152:NCGR1603_wounded_inoculated_R3