organism: Schizosaccharomyces pombe
description: Protocols: Samples were prepared from 3 biological replicates of mga2Δ (MP815) and mga2Δcbf11Δ (MP836) cells. Cells were cultured to exponential phase in the complex YES medium (with or without ammonium supplementation), 10 mL were harvested (600 g, 2 min) and the cell pellet was flash-frozen. Total RNA was isolated using a hot acidic phenol method followed by phenol-chloroform extractions and precipitation (PMID: 12854975). Extracted RNA was treated with TURBO DNase (Thermo Fisher Scientific) and purified using Qiagen RNeasy columns. RNA quality was assessed on Agilent Bioanalyzer. The sequencing libraries were synthesized using KAPA mRNA HyperPrep Kit (Illumina platform) (Roche, KK8581).
attributes.tag: ENA first public
attributes.value: 2024-04-05
attributes.tag: External Id
attributes.value: SAMEA114307703
attributes.tag: INSDC center name
attributes.value: Charles University, Faculty of Science Department of Cell Biology
attributes.tag: INSDC last update
attributes.value: 2023-08-29T10:12:05Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-13303:A8
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: fission yeast
attributes.tag: genotype
attributes.value: mga2_cbf11
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: growth condition
attributes.value: YES medium with ammonium chloride
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: whole organism
attributes.tag: replicate
attributes.value: 2
attributes.tag: sample name
attributes.value: E-MTAB-13303:A8
attributes.tag: scientific_name
attributes.value: Schizosaccharomyces pombe
attributes.tag: strain
attributes.value: MP836