organism: Macaca fascicularis
description: Protocols: macaque monkeys (6–18 years old) were used. Seven to nine sections were combined from one individual for nucleus isolation. The isolation protocol mainly comes from the published 10x Genomics protocol for nucleus isolation. In brief, the tissue was lysed via a chilled lysis kit (Sigma, no. NUC101-1KIT ). The suspension was then filtered with cell strainer and washed impurities by centrifugation with different concentrations of iodixanol. Nuclei-pellets were resuspended with a “resuspension buffer” which contained 1× phosphate-buffered saline (PBS), 1% bovine serum albumin (BSA) and 0.2 U/μl RNase inhibitor. Nuclei-pellets were suspended in the DAPI solution and incubated for inspection under the microscope. NA NA MPTP (3mg; Sigma-Aldrich, St. Louis, MO, USA) was dissolved into a concentration of 0.15mg/ml and injected singly into the left carotid artery. NA 10X single cell isolation protocol 10X Genomics Chromium platform
attributes.tag: disease
attributes.value: Parkinson's disease
attributes.tag: organism
attributes.value: Macaca fascicularis
attributes.tag: organism
attributes.value: Macaca fascicularis
attributes.tag: collection date
attributes.value: not collected
attributes.tag: sex
attributes.value: male
attributes.tag: stimulus
attributes.value: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine injection
attributes.tag: scientific_name
attributes.value: Macaca fascicularis
attributes.tag: common name
attributes.value: crab-eating macaque
attributes.tag: organism part
attributes.value: brain
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-11-19T14:00:06Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-11-26T00:27:11Z
attributes.tag: age
attributes.value: 12
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-11-19
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-11-26