organism: Mus musculus
description: Protocols: LECs were isolated from tumor-draining inguinal LNs of C57Bl/6N wild type mice. 200,000 B16F10-luc2 cells were implanted intradermally 14 days prior to sacrifice Cells were sorted into 384-well plates containing lysis buffer and RNA was extracted. Libraries were prepared using a miniaturised version of the Smart-seq2 protocol (Picelli et al., 2014).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-10-19
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-12-30
attributes.tag: External Id
attributes.value: SAMEA13424428
attributes.tag: INSDC center alias
attributes.value: Post-doc, Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zurich
attributes.tag: INSDC center name
attributes.value: Post-doc, Institute of Pharmaceutical Sciences, Department of Chemistry and Applied Biosciences, ETH Zurich
attributes.tag: INSDC first public
attributes.value: 2022-10-19T22:02:54Z
attributes.tag: INSDC last update
attributes.value: 2022-12-30T00:24:03Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11524:Sample 169
attributes.tag: age
attributes.value: 8 to 10
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_type
attributes.value: endothelial cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: growth condition
attributes.value: injected with B16F10-luc2 melanoma cells
attributes.tag: individual
attributes.value: from the same pool of mice
attributes.tag: organism part
attributes.value: inguinal lymph node
attributes.tag: post analysis well quality
attributes.value: fail
attributes.tag: sample name
attributes.value: E-MTAB-11524:Sample 169
attributes.tag: sex
attributes.value: female
attributes.tag: single cell well quality
attributes.value: not OK
attributes.tag: strain
attributes.value: C57BL/6N