organism: Escherichia coli K-12
description: Protocols: ~210 000 colonies were scraped from the LB agar plate containing appropriate antibiotics total gDNA was purified by GenElute Bacterial Genomic DNA kit (Sigma-Aldrich, St. Louis, MO) Library preparation was carried out as described by Espinosa et al. (https://doi.org/10.1016/j.xpro.2020.100202). In brief, DNA was first digested with MmeI. Fragment containing Tn was size-fractioned and then adaptor-ligated. PCR was carried out to add appropriate adapters while amplify the target. Size-fractioned and purified PCR product was subjected to sequencing.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-12-31
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-12-31
attributes.tag: External Id
attributes.value: SAMEA14369902
attributes.tag: INSDC center alias
attributes.value: Institute for Integrative Biology of the Cell (I2BC)
attributes.tag: INSDC center name
attributes.value: Institute for Integrative Biology of the Cell (I2BC)
attributes.tag: INSDC first public
attributes.value: 2022-12-31T00:58:19Z
attributes.tag: INSDC last update
attributes.value: 2022-12-31T00:58:19Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11704:R388 Exconjugant #1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: genotype
attributes.value: MC1061::Tn / R388
attributes.tag: individual
attributes.value: YAM39
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: sample name
attributes.value: E-MTAB-11704:R388 Exconjugant #1