organism: Hypericum perforatum
description: Protocols: About 100 mg of cells or plantlets were collected and powdered using liquid nitrogen. Plantlets were maintained in MS liquid medium augmented with 0.1 mg/L NAA and 1 mg/L 6-benzyladenine. Cell suspension cultures were maintained in Erlenmeyer flasks (250 ml) containing Murashige and Skoog's (MS) medium (Duchefa Biochemie, Haarlem, Netherlands) augmented with 0.5 mg/L α-naphthaleneacetic acid (NAA) on an Orbi-Shaker (Benchmark Scientific, USA) at 110 rpm kept in a growth chamber programmed to provide 16/8 (day/night) photoperiod, 80 µmol m−2 s−1 light intensity, 70 % relative humidity and 25 °C temperature. To keep the cells in growth phase, 10 ml of grown culture was subcultured to 70 ml of fresh medium once in 7 days. Total RNA was isolated using Sigma spectrum RNA extraction kit (Sigma-Aldrich, USA). On column DNase treatment (Sigma-Aldrich, USA) was given to clean the DNA contamination. Suppliers' information was followed for extraction and purification, and the RNA was eluted in 100 µl elution buffer provided in the kit. Quantity of the RNA was measured using NanoDrop™ OneC and the RNA integrity was measured using Agilent Bioanalyzer 2100. Iso-Seq library preparation was done using PacBio full-length cDNA library preparation kit according to the manufacture's protocol (Pacific Biosciences of California, Inc., Menlo Park, CA, USA) by the sequencing service provider.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-12-31
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-12-31
attributes.tag: External Id
attributes.value: SAMEA12921932
attributes.tag: INSDC center alias
attributes.value: Department of Biometry and Bioinformatics, Institute of Plant Genetics, Polish Academy of Sciences
attributes.tag: INSDC center name
attributes.value: Department of Biometry and Bioinformatics, Institute of Plant Genetics, Polish Academy of Sciences
attributes.tag: INSDC first public
attributes.value: 2022-12-31T00:57:49Z
attributes.tag: INSDC last update
attributes.value: 2022-12-31T00:57:49Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11423:Sample 1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cultivar
attributes.value: Helos
attributes.tag: developmental stage
attributes.value: vegetative
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: plantlet
attributes.tag: replicate
attributes.value: 1
attributes.tag: sample name
attributes.value: E-MTAB-11423:Sample 1