organism: Bos taurus
description: Protocols: To identify expression quantitative trait loci (eQTL) in Hanwoo cattle, 32 animals were provided by the Hanwoo cattle Research institute, National Institute of Animal Science, South Korea. For RNA-sequencing, tissue samples, such as Abdominal Fat (ABF), Back Fat (BFT) and Kidney Fat (KIF) were collected from the castrated Hanwoo cattle after slaughtering at 10~26 months of age. Ethics approval was obtained from the National Institute of Animal Science (approval no: NIAS20201979). Total RNA was extracted from the trachea with TRIzol Reagent (Invitrogen, Carlsbad, CA, USA) following the manufacturer's protocol. RNA concentration was checked using a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies, USA). Individual libraries were generated using Illumina TruSeq™ RNA Sample Preparation Kit (Illumina, San Diego, CA, USA).
attributes.tag: ENA first public
attributes.value: 2024-03-21
attributes.tag: External Id
attributes.value: SAMEA114501075
attributes.tag: INSDC center name
attributes.value: Gyeongsang National University Animal Genomics Laboratory
attributes.tag: INSDC last update
attributes.value: 2023-10-16T16:10:00Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-13398:192019
attributes.tag: age
attributes.value: 22
attributes.tag: body weight
attributes.value: 481
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: cattle
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: Abdominal fat; Back fat; Kidney fat
attributes.tag: phenotype
attributes.value: control
attributes.tag: sample name
attributes.value: E-MTAB-13398:192019
attributes.tag: scientific_name
attributes.value: Bos taurus
attributes.tag: sex
attributes.value: castrated male
attributes.tag: strain
attributes.value: Hanwoo cattle (Korean native cattle)