organism: Mus musculus
description: Protocols: Transcardial aCSF perfusion, brain extraction and sectioning, microdissection of full amygdala, single-cell dissociation, scRNA-seq; as described in Zeisel et al. 2018 7-11 week old male C57Bl/6JOlaHsd mice were habituated to a behavioral arena for 20min, followed by 6 cycles of (foot shock-tone shock (US-CS) pairing: 35sec tone (CS), 750ms 0.7mV foot shock (US), 24sec rest. For learning and consolidation, mice were sampled 2h, 8h and 24h after tone-cued fear condition (CFC). For recall, 24h after CFC, 4 mice were exposed to 6 cycles of the tone cue alone (35sec CS, 25sec rest). The 3 mice exhibiting strongest freezing behavior compared to non-FC controls undergoing the same session were sacrificed 2h later. Naïve, home cage (HC) littermates served as controls in each experimental batch. 10Xgenomics 3' V3 10Xgenomics 3' V3
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-01-01
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-01-01
attributes.tag: External Id
attributes.value: SAMEA110663756
attributes.tag: INSDC center alias
attributes.value: Technion, Israel
attributes.tag: INSDC center name
attributes.value: Technion, Israel
attributes.tag: INSDC first public
attributes.value: 2023-01-01T00:28:27Z
attributes.tag: INSDC last update
attributes.value: 2023-01-01T00:28:27Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12096:10_1
attributes.tag: age
attributes.value: 10
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_type
attributes.value: neural cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: amygdala
attributes.tag: sample name
attributes.value: E-MTAB-12096:10_1
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: C57BL/6J