organism: Schizosaccharomyces pombe
description: Protocols: Samples were prepared from 3 biological replicates. Two separate experiments were performed: 1) Cells were cultured to exponential phase in the complex YES medium with or without ammonium supplementation. 2) Cells were cultured to exponential phase in the complex YES medium and then treated with DMSO (control) or 20 μM cerulenin for 1 hour. For each sample, 10 mL were harvested (600 g, 2 min) and the cell pellet was flash-frozen. Total RNA was isolated using a hot acidic phenol method followed by phenol-chloroform extractions and precipitation (PMID: 12854975). Extracted RNA was treated with TURBO DNase (Thermo Fisher Scientific) and purified using Qiagen RNeasy columns. RNA quality was assessed on Agilent Bioanalyzer. The sequencing libraries were synthesized using KAPA mRNA HyperPrep Kit (Illumina platform) (Roche, KK8581).
attributes.tag: ENA first public
attributes.value: 2024-04-05
attributes.tag: External Id
attributes.value: SAMEA114307743
attributes.tag: INSDC center name
attributes.value: Charles University, Faculty of Science Department of Cell Biology
attributes.tag: INSDC last update
attributes.value: 2023-08-29T10:43:38Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-13305:V1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: fission yeast
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: growth condition
attributes.value: YES medium
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: whole organism
attributes.tag: replicate
attributes.value: 1
attributes.tag: sample name
attributes.value: E-MTAB-13305:V1
attributes.tag: scientific_name
attributes.value: Schizosaccharomyces pombe
attributes.tag: strain
attributes.value: JB32