organism: Escherichia coli
description: Sequencing of single colony isolates of RNR31 evolved for 30 transfers in MOPS + 1% glucose + 1 mg/ml dNS + 2 ug/ml Streptomycin + 1 ug/ml Chloramphenicol. (Replicate Lines 1-5)
attributes.tag: strain
attributes.value: B str. RNR31
attributes.tag: collection_date
attributes.value: 2021
attributes.tag: env_broad_scale
attributes.value: not applicable
attributes.tag: env_local_scale
attributes.value: not applicable
attributes.tag: env_medium
attributes.value: not applicable
attributes.tag: geo_loc_name
attributes.value: New Zealand: Auckland
attributes.tag: isol_growth_condt
attributes.value: not applicable
attributes.tag: lat_lon
attributes.value: not applicable
attributes.tag: num_replicons
attributes.value: not applicable
attributes.tag: ref_biomaterial
attributes.value: not applicable
attributes.tag: encoded_traits
attributes.value: Streptomycin resistance; Chloramphenicol resistance
attributes.tag: extrachrom_elements
attributes.value: pBAD33::Ptac–mm-dAK–rrnBt
attributes.tag: host
attributes.value: not applicable
attributes.tag: isolation_source
attributes.value: Laboratory
attributes.tag: experiment
attributes.value: deltaRNR T30 dNS 1mg/ml
attributes.tag: BioSampleModel
attributes.value: MIGS.ba