organism: Mus musculus
description: Protocols: The microdissected spinal cord central canal region from each mouse was dissociated into a cell suspension (two cell preparations each day, one from a young adult mouse and one from an aged mouse). Cells from each mouse were loaded into a well of the Chromium Next GEM chip (10x Genomics, cat# 1000127) and then into the Chromium Single Cell controller (10x Genomics) to partition cells in GEMs (Gel Beads-in-emulsion). Nuclei acid extraction was performed following the manufacturer's instructions (10x Genomics, as part of the Chromium Single Cell 3' v3.1). Libraries were generated using the Chromium Next GEM Single Cell 3ʹ GEM, Library & Gel Bead Kit v3.1 (cat# 1000092) following the manufacturer's instructions (10x Genomics).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-12-31
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-12-31
attributes.tag: External Id
attributes.value: SAMEA13364037
attributes.tag: INSDC center alias
attributes.value: Division of Cell and Developmental Biology, School of Life Sciences, University of Dundee
attributes.tag: INSDC center name
attributes.value: Division of Cell and Developmental Biology, School of Life Sciences, University of Dundee
attributes.tag: INSDC first public
attributes.value: 2022-12-31T00:58:02Z
attributes.tag: INSDC last update
attributes.value: 2022-12-31T00:58:02Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11561:10X03_1
attributes.tag: age
attributes.value: 112
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_type
attributes.value: ependymal cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: individual
attributes.value: 436219
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: spinal cord
attributes.tag: sample name
attributes.value: E-MTAB-11561:10X03_1
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: C57BL/6J