organism: Mus musculus
description: Protocols: The mice were fasted overnight (11 hours) prior to liver isolation, and livers were kept at -80°C until analysis. For eight weeks, female C57BL/6JRj mice (seven weeks old at the start of treatment) received, by subcutaneous injections, either a glucagon receptor antibody (GCGR Ab, REGN1193, 10 mg/kg body weight; Regeneron; IC50: 3.5 pM (Okamoto et al., 2015)) or a control antibody (Ctl Ab, REGN1945, 10 mg/kg body weight; Regeneron). The antibodies were administered once weekly (Wednesdays at 8 AM). The last doses of GCGR Ab and Ctl Ab were given one week prior to termination. Raw sequencing data were processed using bcl2fastq software version 2.20.0 (Illumina). Total DNA/RNA was purified by an AllPrep DNA/RNA Minikit (80204, QIAgen) according to manufacturer's instructions and quality tested using a 2100 Bioanalyzer instrument (Agilent Genomics). The extract was DNase treated with RNeasy Minikit (74106, QIAgen) and RNase-Free DNase set (79254, QIAgen) according to manufacturer's protocol. All the above-mentioned processes were performed on a QIACube machine (QIAgen). Library preparation was done using TruSeq Stranded Total RNA Library Prep Gold (20020599, Illumina) and IDT for Illumina-TruSeq RNA UD Indexes (20022371, Illumina) according to the manufacturer's instructions. Some samples (input <100 ng) were prepared with NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB-E7760S, New England Biolabs), NEBNext rRNA Depletion Kit 24 with beads (NEB-E7765, New England Biolabs), and NEBNext Multiplex Oligos for Illumina (E7600S, New England Biolabs).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-10-12T09:00:10Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-07-01T00:20:14Z
attributes.tag: External Id
attributes.value: SAMEA110473259
attributes.tag: INSDC center alias
attributes.value: Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen
attributes.tag: INSDC center name
attributes.value: Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen Novo Nordisk Foundation Center for Basic Metabolic Research, Faculty of Health and Medical Sciences, University of Copenhagen
attributes.tag: INSDC first public
attributes.value: 2022-10-12T09:00:10Z
attributes.tag: INSDC last update
attributes.value: 2023-07-01T00:20:14Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12048:Sample 68
attributes.tag: age
attributes.value: 15
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: liver
attributes.tag: sample name
attributes.value: E-MTAB-12048:Sample 68
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: C57BL/6JRj