organism: Oryza sativa Japonica Group
description: Protocols: Root hair samples were collected from 3~4 days-old seedlings that germinated on hard MSO agar plates. Collar, Leaf sheath, and Ligule were collected from 5~6 weeks old plants grown at the greenhouse. Lastly, anther wall samples were collected from the paddy field after releasing pollen. Root hair) 3-4days-old seedlings were grown on hard MSO agar plate Collar, Leaf sheath, and Ligule) After two weeks in incubator at BOD incubator, the plants were grown in the greenhouse for a month. Anther wall) Plants were transplanted to paddy field after two weeks in incubator at BOD incubator at end of May. The plants were then grown for 3 months in the field. We extract RNA from the sample using RNeasy Plant Mini Kit from QIAGEN following manufacturer's instruction. TruSeq Stranded mRNA Sample Preparation Guide, Part #15031047 Rev. E with TruSeq Stranded mRNA LT Sample Prep Kit
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-12-31
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-12-31
attributes.tag: External Id
attributes.value: SAMEA10270011
attributes.tag: INSDC center alias
attributes.value: KYUNG HEE UNIVERSITY
attributes.tag: INSDC center name
attributes.value: KYUNG HEE UNIVERSITY
attributes.tag: INSDC first public
attributes.value: 2022-12-31T00:57:11Z
attributes.tag: INSDC last update
attributes.value: 2022-12-31T00:57:11Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11005:Collar_3
attributes.tag: age
attributes.value: 5 to 6
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: Japanese rice
attributes.tag: cultivar
attributes.value: Dongjin
attributes.tag: developmental stage
attributes.value: sporophyte vegetative stage
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: leaf collar
attributes.tag: sample name
attributes.value: E-MTAB-11005:Collar_3