organism: Homo sapiens
description: Protocols: LU65 cells and MiaPaCa2 cells were grown in increasing dose of Sotorasib for more than 1 month to establish sotorasib-resistant cells. Cell pellets of parental and sotorasib-resistant cells were collected. Sotorasib-resistant cells were grown in culture media with 5 micromole sotorasib Genomic DNA was extracted using DNA purification kits from Qiagen 100ng of DNA was used for DNA library preparation with Illumina DNA Prep with Enrichment kit
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-01-01
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-01-01
attributes.tag: External Id
attributes.value: SAMEA111329120
attributes.tag: INSDC center alias
attributes.value: National Taiwan University Hospital
attributes.tag: INSDC center name
attributes.value: National Taiwan University Hospital
attributes.tag: INSDC first public
attributes.value: 2023-01-01T00:28:36Z
attributes.tag: INSDC last update
attributes.value: 2023-01-01T00:28:36Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12144:MIAPaCa2 parental cells
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: MIA Paca-2
attributes.tag: cell_type
attributes.value: pancreatic ductal cell
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: pancreatic carcinoma
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: phenotype
attributes.value: parental line
attributes.tag: sample name
attributes.value: E-MTAB-12144:MIAPaCa2 parental cells