organism: Homo sapiens
description: Protocols: Cells were harvested and FACS sorted (FACSAria™ III (BD Biosciences)) for live cells before nucleic acid extraction CD8+ T cells were purified from human blood via buffy coat and purification of CD8+ positive cells using MACS and cultured (3-4 million) separately in plasma group(1ml) and ascites group(1ml) with activation beads ratio(cell : beads=2:1) for 16hr. Qiagen, RNeasy Plus Micro Kit Cat# 74034 Lexogen Quantseq
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-12-31
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-12-31
attributes.tag: External Id
attributes.value: SAMEA111329154
attributes.tag: INSDC center alias
attributes.value: Philipps University Marburg, Bioinformatics Core Facility, Center for Tumor- and Immune Biology,
attributes.tag: INSDC center name
attributes.value: Philipps University Marburg, Bioinformatics Core Facility, Center for Tumor- and Immune Biology,
attributes.tag: INSDC first public
attributes.value: 2022-12-31T00:58:36Z
attributes.tag: INSDC last update
attributes.value: 2022-12-31T00:58:36Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12141:YN047
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_type
attributes.value: CD8-Positive T-Lymphocytes
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: individual
attributes.value: 20
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: blood
attributes.tag: sample name
attributes.value: E-MTAB-12141:YN047