organism: wastewater metagenome
attributes.tag: collection_date
attributes.value: 2022-08-27
attributes.tag: geo_loc_name
attributes.value: USA: Wisconsin
attributes.tag: isolation_source
attributes.value: wastewater
attributes.tag: ww_population
attributes.value: <100000
attributes.tag: ww_sample_duration
attributes.value: 24
attributes.tag: ww_sample_matrix
attributes.value: raw wastewater
attributes.tag: ww_sample_type
attributes.value: composite
attributes.tag: purpose_of_ww_sampling
attributes.value: public health surveillance community-level
attributes.tag: purpose_of_ww_sequencing
attributes.value: Identification of the diversity of mutations across the SARS-CoV-2 metagenome
attributes.tag: sequenced_by
attributes.value: Wisconsin State Laboroatory of Hygiene
attributes.tag: ww_endog_control_1
attributes.value: pepper mild mottle virus
attributes.tag: ww_endog_control_1_conc
attributes.value: 264320000
attributes.tag: ww_endog_control_1_protocol
attributes.value: IDT PMMoV primer-probe set described in PMID 24056678 measured using dPCR QIAcuity instrument 96W plates (Qiagen).
attributes.tag: ww_endog_control_1_units
attributes.value: copies/L
attributes.tag: ww_flow
attributes.value: 1760216.487
attributes.tag: ww_ph
attributes.value: 7.9
attributes.tag: ww_population_source
attributes.value: population of jurisdiction encompassing the wastewater service area
attributes.tag: ww_primary_sludge_retention_time
attributes.value: not applicable
attributes.tag: ww_processing_protocol
attributes.value: Internal control spiked in samples at receipt. Samples concentrated using Nanotrap ER2 (Ceres) on KingFisher Apex and extracted using Maxwell HT viral TNA kit (Promega) on KingFisher Flex.
attributes.tag: ww_sample_site
attributes.value: wastewater treatment plant
attributes.tag: ww_surv_jurisdiction
attributes.value: WI
attributes.tag: ww_surv_system_sample_id
attributes.value: 639790001
attributes.tag: project_name
attributes.value: Wisconsin SARS-CoV-2 Wastewater Monitoring Network
attributes.tag: concentration_method
attributes.value: ceres nanotrap
attributes.tag: extraction_method
attributes.value: promega ht tna kit
attributes.tag: extraction_control
attributes.value: bovine coronavirus
attributes.tag: ww_surv_target_1
attributes.value: SARS-CoV-2
attributes.tag: ww_surv_target_1_gene
attributes.value: N gene (N1)
attributes.tag: ww_surv_target_1_known_present
attributes.value: yes
attributes.tag: ww_surv_target_1_protocol
attributes.value: IDT SARS-CoV-2 Wastewater Surveillance CDC Assay For QIAcuity dPCR 24W (Qiagen)
attributes.tag: ww_surv_target_1_conc
attributes.value: 291840
attributes.tag: ww_surv_target_1_conc_unit
attributes.value: copies/L
attributes.tag: ww_surv_target_2
attributes.value: SARS-CoV-2
attributes.tag: ww_surv_target_2_gene
attributes.value: N gene (N2)
attributes.tag: ww_surv_target_2_conc
attributes.value: 309120
attributes.tag: ww_surv_target_2_conc_unit
attributes.value: copies/L
attributes.tag: ww_surv_target_2_known_present
attributes.value: yes
attributes.tag: ww_surv_target_2_protocol
attributes.value: IDT SARS-CoV-2 Wastewater Surveillance CDC Assay For QIAcuity dPCR 24W (Qiagen)
attributes.tag: BioSampleModel
attributes.value: SARS-CoV-2: wastewater surveillance