organism: Mus musculus
description: Protocols: Mice were sacrificed and mammary gland tumors collected and frozen. Eight-week-old female mice (FVB/NCrl, Charles River) were engrafted with 5 × 106 NT193 pool cells (diluted in 50 μl filter-sterilized PBS) in the surgically exposed left fourth mammary gland. After a period of 2 weeks (when the tumors were around 200 mm3), the mice received treatment with MP5 at a dosage of 1 mg/kg (in 50 μl PBS) or PBS control through peritumoral injections every second day for a total of 10 injections over 3 weeks. Total RNA was prepared from frozen tissues using TriReagent (Life Technologies) with a tissue homogenizer according to the manufacturer's instructions. Sequencing libraries were prepared using the Smarter Stranded Total RNA-Seq kit v3 Pico Input Mammalian following the manufacturer's instructions (Takara Bio, San Jose, CA, USA).
attributes.tag: ENA first public
attributes.value: 2024-01-20
attributes.tag: INSDC center name
attributes.value: University of Strasbourg
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-13677:TENASCIN_3_MP5-3_BM1_10_R1_T1
attributes.tag: age
attributes.value: 8
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: individual
attributes.value: MP5-3
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: mammary gland
attributes.tag: sample name
attributes.value: E-MTAB-13677:TENASCIN_3_MP5-3_BM1_10_R1_T1
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: FVB/NCrl