organism: pig gut metagenome
description: Feaces_Before_Zinc_mix
attributes.tag: environment (material)
attributes.value: Faeces
attributes.tag: collection date
attributes.value: 2020
attributes.tag: target gene
attributes.value: 16S: V4- 250 bp
attributes.tag: geographic location (longitude)
attributes.value: -5.936.998
attributes.tag: host common name
attributes.value: pig
attributes.tag: host-associated environmental package
attributes.value: host-associated
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-12-09T00:31:33Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-12-09T00:31:33Z
attributes.tag: nucleic acid extraction
attributes.value: Fast DNA SPIN Kit for Soil
attributes.tag: nucleic acid amplification
attributes.value: PCR
attributes.tag: environment (biome)
attributes.value: swine
attributes.tag: environment (feature)
attributes.value: GIT
attributes.tag: geographic location (country and/or sea)
attributes.value: United Kingdom
attributes.tag: sample storage temperature
attributes.value: -80
attributes.tag: adapters
attributes.value: Illumina adapters
attributes.tag: organism
attributes.value: pig gut metagenome
attributes.tag: geographic location (latitude)
attributes.value: 54.579.058
attributes.tag: scientific_name
attributes.value: pig gut metagenome
attributes.tag: common name
attributes.value: pig
attributes.tag: geographic location (region and locality)
attributes.value: Belfast
attributes.tag: investigation type
attributes.value: bacteria_archaea
attributes.tag: pcr primers
attributes.value: 515F and 806R
attributes.tag: pcr conditions
attributes.value: 95�C for 3 minutes, 20 cycles at 95�C for 30 seconds, 55�C for 30 seconds, 72�C for 30 seconds and then 72�C for 5 minutes.
attributes.tag: project name
attributes.value: Affect_of_zinc_and_alternative_compound_on pigs'_microbiota
attributes.tag: sequencing method
attributes.value: Illumina MiSeq
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-12-09
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-12-09