organism: Homo sapiens
description: Protocols: Eosinophils were purified from the peripheral blood of healthy controls and asthmatic donors. For eosinophil purification, CD2, CD3, CD14, CD16, CD19, CD20, CD36, CD56, CD123, and glycophorin A positive cells were discarded by a magnetic bead separation technique, as described in the manufacturer's procedure (EasySep; StemCell Technologies, Vancouver, Canada). Eosinophils whole transcriptome analyses were performed using the BD Rhapsody™ Single Cell Analysis System (BD Biosciences). Purified eosinophils (120000 cells) were centrifuged and resuspended in 650 μL of BD sample buffer supplemented with 1:1000 SUPERase-In (20 U/μL; AM2694 ThermoFisher) and NxGen Rnase Inhibitors (40 U/μL; 30281-2 Lucigen), as previously described. For each sample, 20000-40000 cells were loaded into a different Rhapsody Single-Cell Analysis System cartridge. Cell capture and cDNA synthesis were performed according to manufacturer instructions.
attributes.tag: disease
attributes.value: normal
attributes.tag: organism
attributes.value: Homo sapiens
attributes.tag: organism
attributes.value: Homo sapiens
attributes.tag: collection date
attributes.value: not collected
attributes.tag: sex
attributes.value: female
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: common name
attributes.value: human
attributes.tag: organism part
attributes.value: blood
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: cell_type
attributes.value: eosinophil
attributes.tag: age
attributes.value: 46
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected