organism: Homo sapiens
description: Protocols: Fifty-two formalin-fixed, paraffin-embedded (FFPE) samples from 52 patients with advanced melanoma and treated with anti-PD1 inhibitors were recruited by the Spanish Group of Melanoma (GEM) for this study. Five to ten 10-15 µm FFPE sections were obtained for each sample. Total RNA was isolated using miRNeasy FFPE Kit (Qiagen) following manufacturer's instructions. Purified nucleic acid quality control for quantity and purity was assessed using an ND-1000 NanoDrop spectrophotometer (Thermo Fisher Scientific). 100 ng of RNA from each sample were used for library preparation with the KAPA RNA Hyperprep kit (Roche Nimblegen Inc.) following manufacturer's instructions. Library fragments distribution was confirmed by electrophoresis and concentration was determined using the KAPA library Quantification kit (Roche Nimblegen Inc.). A seven MB SeqCap EZ probe pool (Roche), including genes previously defined, was designed using the NimbleDesign online tool. An equal mass of eight cDNA libraries was pooled and hybridized with the SeqCap EZ probe pool following manufacturers' specifications.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-12-31
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-12-31
attributes.tag: External Id
attributes.value: SAMEA14381273
attributes.tag: INSDC center alias
attributes.value: Fundacion Parque Cientifico de MAdrid
attributes.tag: INSDC center name
attributes.value: Fundacion Parque Cientifico de MAdrid
attributes.tag: INSDC first public
attributes.value: 2022-12-31T00:58:19Z
attributes.tag: INSDC last update
attributes.value: 2022-12-31T00:58:19Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11729:Sample 27
attributes.tag: age
attributes.value: 50
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: clinical information
attributes.value: treatment with anti-PD1 inhibitors
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: melanoma
attributes.tag: individual
attributes.value: GEM038
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: tumor
attributes.tag: sample name
attributes.value: E-MTAB-11729:Sample 27
attributes.tag: sex
attributes.value: male