organism: Homo sapiens
description: Protocols: Samples were inoculated in DMEM with 10% FBS for 24 hours. After that time, samples were cultured for 72 hours in a) DMEM with 10% Exosome depleted serum (EDS) [control]; b) DMEM with 10% EDS + 10 microg/ml G-361 exosomes [EXO_G361]; c) DMEM with 10% EDS + 10 microg/ml exosomes from FBS [EXO_FBS]. Total RNA was isolated by the Qiagen RNeasy Micro Kit (Qiagen), according to the manufacturer's protocol, including treating samples by DNase I. For the library construction, KAPA mRNA HyperPrep Kit with Poly(A) mRNA selection (Roche) was used with starting amount of 1000 ng of total RNA.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-12-31
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-12-31
attributes.tag: External Id
attributes.value: SAMEA8425946
attributes.tag: INSDC center alias
attributes.value: Laboratory of Genomics and Bioinformatics, Institute of Molecular Genetics of the Czech Academy of Sciences
attributes.tag: INSDC center name
attributes.value: Laboratory of Genomics and Bioinformatics, Institute of Molecular Genetics of the Czech Academy of Sciences
attributes.tag: INSDC first public
attributes.value: 2022-12-31T00:56:38Z
attributes.tag: INSDC last update
attributes.value: 2022-12-31T00:56:38Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-10278:mCAF_EXO_G361
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: fibroblast derived cell line
attributes.tag: cell_type
attributes.value: mCAF
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: cutaneous melanoma
attributes.tag: experimental factor
attributes.value: EXO G361
attributes.tag: genotype
attributes.value: mCAF
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: material type[2
attributes.value: total RNA
attributes.tag: organism part
attributes.value: melanoma
attributes.tag: replicate
attributes.value: 1
attributes.tag: sample name
attributes.value: E-MTAB-10278:mCAF_EXO_G361