organism: Macaca fascicularis
description: Protocols: Total 8 monkeys (4-15 years old, 8 males and 2 females, Macaca fascicularis) were used in the current project. All procedures complied with the NIH Guide for the Care and Use of Laboratory Animals approved by Sun Yat-sen University. Following 2 weeks of adaptive housing in the institutional animal facility, animals were anesthetized with a lethal dose of sodium pentobarbital. Animals were transcardially perfused with 2 liters of artificial cerebrospinal fluid (ACSF, in mM: 125 NaCl, 25 NaHCO3, 1 NaH2PO4, 2 KCl, 25 D-Glucose, 2 CaCl2, 1 MgCl2, 5 sodium l-ascorbate, 3 sodium pyruvate, 0.01 taurine, 2 thiourea, 1 kynurenic acid, 0.1 DL-AP5, and 1x10-3 tetrodotoxin). The flow of perfusion fluid was adjusted so that the perfusion finished within approximately 40 minutes. The brain was removed from the skull immediately and a block of tissue containing the hippocampus was cut off. The position of hippocampus referred to the atlas of the rhesus monkey brain (Saleem and Logothetis, 2012). The tissue block was kept in the ACSF and sectioned on a vibratome (VT1200S, Leica) in 300 um thick slices. The adherent tissues around the hippocampus of each slice were removed on ice and only the regions of interest were kept for dissociation. 10X single cell isolation protocol v2 10X Genomics Chromium platform v2
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-01-01
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-01-01
attributes.tag: External Id
attributes.value: SAMEA110452525
attributes.tag: INSDC center alias
attributes.value: Sun Yat-sun University Zhongshan Ophathlamic Center
attributes.tag: INSDC center name
attributes.value: Sun Yat-sun University Zhongshan Ophathlamic Center
attributes.tag: INSDC first public
attributes.value: 2023-01-01T00:28:22Z
attributes.tag: INSDC last update
attributes.value: 2023-01-01T00:28:22Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-102251659426996:FMHippo_0801_H1-1
attributes.tag: age
attributes.value: 13
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: crab-eating macaque
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: individual
attributes.value: FM_0801
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: brain
attributes.tag: sample name
attributes.value: E-MTAB-102251659426996:FMHippo_0801_H1-1
attributes.tag: sampling site
attributes.value: hippocampus
attributes.tag: sex
attributes.value: male