organism: Arabidopsis thaliana
description: Protocols: Total RNA was isolated from two-week-old wild-type and atnusg mutant young leaves. For total RNA isolation, frozen sample was ground in liquid nitrogen, and total RNA were extracted using Trizol reagent plus RNeasy mini kit,according to the manufacturer's instructions mRNA was enriched using oligo-(dT) primer for nuclear-encoded genes and reverse-transcribed using random hexamers primers. The double-stranded cDNA was purified using AMPure XP beads and treated with End Prep Enzyme Mix to repair both ends and to add a dA-tail in one reaction, followed by T-A ligation to add adaptors to both ends. Size selection of adaptor-ligated DNA was performed using AMPure XP beads, and ~360-bp fragments (with an approximate insert size of 300 bp) were recovered. The library was then constructed and sequenced using an Illumina platform
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-08-30
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-08-30
attributes.tag: External Id
attributes.value: SAMEA10157785
attributes.tag: INSDC center alias
attributes.value: Institute of Botany, the Chinese Academy of Sciences, Beijing
attributes.tag: INSDC center name
attributes.value: Institute of Botany, the Chinese Academy of Sciences, Beijing
attributes.tag: INSDC first public
attributes.value: 2022-08-30T00:12:29Z
attributes.tag: INSDC last update
attributes.value: 2022-08-30T00:12:29Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-10957:col_3
attributes.tag: age
attributes.value: 2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: thale cress
attributes.tag: developmental stage
attributes.value: seedling developmental stage
attributes.tag: ecotype
attributes.value: Col-0
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: leaf
attributes.tag: sample name
attributes.value: E-MTAB-10957:col_3