organism: Homo sapiens
description: Protocols: Cord blood (CB) samples (n=3) were harvested in EDTA containing tubes and stored at room temperature. Samples were processed within 2h from collection. We isolated mononuclear cells and granulocytes. From the mononuclear cell fraction, we isolated low density neutrophils (LDN) by performing a double round of magnetic bead sorting using Neutrophil Isolation kit (StemCell Technologies). From the granulocyte cell fraction, we isolated normal density (NDN) by performing a single round of magnetic bead sorting using Neutrophil Isolation kit (StemCell Technologies). LDN and NDN from each CB sample were mixed in a ratio of 1:3. We plated LDN-NDN mix at 10^6 cells/ml in RPMI 1640 + 10% FBS + 1% Gln + 1% Pen/strep alone or with G-CSF, IFN- or IFN- all used at 10ng/ml. After 4h, cells were harvested, washed, counted and for each stimulation condition we mixed cells from each CB in a ratio 1:1:1. The pooled samples were processed for scRNAseq. na
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-09-09
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-09-09
attributes.tag: External Id
attributes.value: SAMEA11305889
attributes.tag: INSDC center alias
attributes.value: San Raffaele Telethon Institute for Gene Therapy (SR-Tiget), IRCCS San Raffaele Scientific Institute
attributes.tag: INSDC center name
attributes.value: San Raffaele Telethon Institute for Gene Therapy (SR-Tiget), IRCCS San Raffaele Scientific Institute
attributes.tag: INSDC first public
attributes.value: 2022-09-09T12:17:12Z
attributes.tag: INSDC last update
attributes.value: 2022-09-09T12:17:12Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11188:Sample 26
attributes.tag: age
attributes.value: 0
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell isolation strategy
attributes.value: Neutrophil Isolation kit
attributes.tag: cell_type
attributes.value: mix of low density neutrophils and normal density neutrophils
attributes.tag: common name
attributes.value: human
attributes.tag: individual
attributes.value: UPN94_95_96_c
attributes.tag: organism part
attributes.value: umbilical cord blood
attributes.tag: sample name
attributes.value: E-MTAB-11188:Sample 26
attributes.tag: sex
attributes.value: not available
attributes.tag: stimulus
attributes.value: IFN-beta