organism: Homo sapiens
attributes.tag: library_ID
attributes.value: VSF02
attributes.tag: isolate
attributes.value: schwannoma
attributes.tag: age
attributes.value: 48
attributes.tag: sex
attributes.value: male
attributes.tag: biomaterial_provider
attributes.value: Raleigh Lab UCSF Helen Diller Family Comprehensive Cancer Center 1450 3rd Street, San Francisco 94182
attributes.tag: tissue
attributes.value: tumor
attributes.tag: platform
attributes.value: ILLUMINA
attributes.tag: instrument_model
attributes.value: Illumina NovaSeq 6000
attributes.tag: design_description
attributes.value: Fresh schwannomas were minced with sterile Bard-Parker #10 surgical scalpels (Aspen Surgical) and incubated in 0.4% Collagenase Type 2 (Worthington) in pre-oxygenated Dulbecco's Modified Eagle Medium (Thermo Fisher Scientific) for 75 minutes at 37°C while rotating at 800 rotations per minute on a thermomixer. The suspension was sequentially filtered through 70 μm and 40 μm strainers (Thermo Fisher Scientific), centrifuged at 300g for 5 minutes, and resuspended in cold phosphate buffered saline. Single cell suspensions were loaded onto a 10x Chromium controller using the Chromium Single Cell 3' Library & Gel Bead Kit v3 (10x Genomics). Libraries were sequenced on an Illumina NovaSeq (10X specific protocol) with >50,000 reads per cell. Library demultiplexing, read alignment to human genome GRCh38 and UMI quantification was performed in Cell Ranger version 1.3.1 (10X Genomics).
attributes.tag: filetype
attributes.value: BAM
attributes.tag: assembly
attributes.value: GRCh38
attributes.tag: filename
attributes.value: VSF02_cell_ranger.bam
attributes.tag: BioSampleModel
attributes.value: Human