organism: Homo sapiens
description: Protocols: 3 normal adrenal gland samples were collected from tissue surrounding endocrine inactive adenoma bearing patients. 3 normal adrenal gland samples were collected from adrenalectomies performed on patients with renal cell carcinoma.7 cortisol producing adenoma samples were collected from patients with hypercortisolism. 5 endocrine inactive adenoma samples were collected from patients with inactive adrenal tumors. Samples were snap-frozen immediately after surgery. Nuclei were isolated from these snap frozen tissues using dounce homogenization and were purified via flow cytometry. The inDrop (1CellBio) workflow was followed to yield barcoded single-nuclei cDNA libraries. Briefly, cells were encapsulated hydrogel beads, droplet oil, RT/lysis mixture and the nuclei. The encapsulated cells were then exposed to UV light, releasing photocleavable primers from hydrogel beads. Subsequently, reverse transcription was performed and the beads filtered out. The RT product was digested using ExoI and HinFI and purified using AMpure XP beads. Then, the second strand cDNA synthesis and in vitro transcription steps were carried out. Next, the resulting RNA molecules were fragmented and a second reverse transcription step was carried out using random hexamers. The cDNA was then purified using Ampure XP beads and quantified via qPCR.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-01-01
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-01-01
attributes.tag: External Id
attributes.value: SAMEA111280418
attributes.tag: INSDC center alias
attributes.value: Max Delbrueck Center for Molecular Medicine Berlin Institute For Medical Systems Biology Free University of Berlin
attributes.tag: INSDC center name
attributes.value: Max Delbrueck Center for Molecular Medicine Berlin Institute For Medical Systems Biology Free University of Berlin
attributes.tag: INSDC first public
attributes.value: 2023-01-01T00:28:35Z
attributes.tag: INSDC last update
attributes.value: 2023-01-01T00:28:35Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12129:CPA- 5
attributes.tag: age
attributes.value: 43
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: cortisol-producing adrenal cortex adenoma
attributes.tag: individual
attributes.value: CPA- 5
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: adrenal gland
attributes.tag: sample name
attributes.value: E-MTAB-12129:CPA- 5
attributes.tag: sampling site
attributes.value: neoplasm
attributes.tag: sex
attributes.value: female