organism: Mus musculus
description: Protocols: There are 4 wild type mice and 4 hypoclycaemic. Each fastq file contains mixed reads from 2 WT and 2 STZ inside (to avoid biases - Biolegend hashing antibodies B0303: control 1, B0304 control 2, B0305 STZ 1, B0306 STZ 2). Sample1: B0303: control 1, B0304 control 2, B0305 STZ 1, B0306 STZ 2 Sample2: B0303: control 3, B0304 control 4, B0305 STZ 3, B0306 STZ 4. Fastq files for hashes are also provided. Mice were made hyperglycaemic using streptozotocin (STZ). Cells were extracted from lungs and subsequently sample was enriched for immune cells using CD45 microbeads from Miltenyi, and with flow cytometry. Libraries were prepared using Chromium Next GEM Single Cell 3聞 Reagent Kits v3.1 with Feature Barcoding technology for Cell Surface Protein Libraries were prepared using Chromium Next GEM Single Cell 3聞 Reagent Kits v3.1 with Feature Barcoding technology for Cell Surface Protein
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-12-31
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-12-31
attributes.tag: External Id
attributes.value: SAMEA12866637
attributes.tag: INSDC center alias
attributes.value: WIS
attributes.tag: INSDC center name
attributes.value: Weizmann Institute of Science
attributes.tag: INSDC first public
attributes.value: 2022-12-31T00:57:48Z
attributes.tag: INSDC last update
attributes.value: 2022-12-31T00:57:48Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11393:Sample 1
attributes.tag: age
attributes.value: 10
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_type
attributes.value: dendritic cell
attributes.tag: common name
attributes.value: house mouse
attributes.tag: compound
attributes.value: streptozotocin
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: immunophenotype
attributes.value: CD45 positive
attributes.tag: individual
attributes.value: mixed of 2 non treated and 2 treated mice
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: lung
attributes.tag: sample name
attributes.value: E-MTAB-11393:Sample 1
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: C57BL/6J