organism: Drosophila melanogaster
description: Protocols: Whole Drosophila heads (N=6-7 heads pooled per sample) from 7-day old females, of the desired genotypes, were dissected and snap frozen for later use. RNA extraction was performed according to the RNAqueous-Micro kit (AM1931). Quality control of RNA was performed using Qubit and tapestation. Next, samples were normalised and library prep was performed using an Illumina TruSeq stranded mRNA kit. This kit involved poly-A enrichment and TruSeq3 adapter ligation.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-01-01
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-01-01
attributes.tag: External Id
attributes.value: SAMEA110664764
attributes.tag: INSDC center alias
attributes.value: School of Medicine, Cardiff University, UK
attributes.tag: INSDC center name
attributes.value: School of Medicine, Cardiff University, UK
attributes.tag: INSDC first public
attributes.value: 2023-01-01T00:28:28Z
attributes.tag: INSDC last update
attributes.value: 2023-01-01T00:28:28Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12090:Cdk12 Control 1
attributes.tag: age
attributes.value: 7
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: fruit fly
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: Sp/CyO;FRT2A, FRT82B/TM3
attributes.tag: individual
attributes.value: mixed pool of 6 to 7 heads
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: head
attributes.tag: sample name
attributes.value: E-MTAB-12090:Cdk12 Control 1
attributes.tag: sex
attributes.value: female