organism: Vaccinia virus Lister
description: Protocols: Virus particles were partially purified from lysates by sucrose gradient centrifugation. RK13 cells were maintained in DMEM + 5% FBS + gentamicin. 48 hours prior to infection, RK13 cells were seeded into 6 well plates at (500000 cells/well). Confluent RK13 cells were infected at MOI = 0.2 (2.40E+05 pfu) Viral genomic DNA was isolated using standard published methods. PacBio CCS
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-09-22T04:31:26Z
attributes.tag: organism
attributes.value: Vaccinia virus Lister
attributes.tag: organism
attributes.value: Vaccinia virus Lister
attributes.tag: passage
attributes.value: 0
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-09-22T04:31:26Z
attributes.tag: scientific_name
attributes.value: Vaccinia virus Lister
attributes.tag: genotype
attributes.value: VC-R2 that lacks both K3L and E3L and expresses EGFP
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-09-22
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-09-22