organism: Mus musculus
description: Protocols: Liver specimens were obtained from QSi5 and 129T2/SvEms mice. Genomic DNA was extracted using the phenol-chloroform extraction method. 200ng of DNA was mechanically fragmented using LE220 Covaris (Covaris, Woburn, USA) to approximately 450 base pair inserts followed by library preparation using the Seqlab TruSeq Nano DNA HT kit (20000903, Illumina, San Diego, USA).    Library preparation was performed according to manufacturer's instructions which included end repair, library size selection, 3' end polyadenylation, adaptor ligation, purification of ligated fragments, enrichment of ligated fragments by PCR and a final purification of amplified DNA library.   Library fragment sizes were reviewed using the LabChip GX (Perkin Elmer, Waltham, USA) to evaluate library size and adapter dimer presence before the library concentration was determined via quantitative real time PCR using the KAPA library quantification kits (KK4824, Roche, Basel, Switzerland) on the QuantStudio7 or ViiA7 (Thermo Fisher Scientific, Waltham, USA). All libraries were normalised before sequencing.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-12-31
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-12-31
attributes.tag: External Id
attributes.value: SAMEA11046380
attributes.tag: INSDC center alias
attributes.value: VICTOR CHANG CARDIAC RESEARCH INSTITUTE
attributes.tag: INSDC center name
attributes.value: VICTOR CHANG CARDIAC RESEARCH INSTITUTE
attributes.tag: INSDC first public
attributes.value: 2022-12-31T00:57:26Z
attributes.tag: INSDC last update
attributes.value: 2022-12-31T00:57:26Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11161:QSi5
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: individual
attributes.value: QSi5
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: liver
attributes.tag: sample name
attributes.value: E-MTAB-11161:QSi5
attributes.tag: sex
attributes.value: not available
attributes.tag: strain
attributes.value: QSi5