organism: Homo sapiens
description: Protocols: RNA were isolated from 4 different HEL cell lines: GFP (CTRL), ETO2-GLIS2, ETO2-GLIS2 C265G and ETO2-GLIS2 dNHR2 following 24h of induction with 500 ng/mL of doxycycline (DOX). RNA were isolated using the RNeasy Mini/Micro Kit (Qiagen) and quantified by NanoDrop (ThermoScientific) cDNA libraries were synthesized from 250 ng total RNA using TruSeq Strandard mRNA Kit (Illumina). Libraries were verified for their amount and quality by capillary electrophoresis using a 2100 Bioanalyzer (Agilent Technologies).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-12-31
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-12-31
attributes.tag: External Id
attributes.value: SAMEA111451407
attributes.tag: INSDC center alias
attributes.value: INSERM Gustave Roussy Cancer Center
attributes.tag: INSDC center name
attributes.value: INSERM Gustave Roussy Cancer Center
attributes.tag: INSDC first public
attributes.value: 2022-12-31T00:58:39Z
attributes.tag: INSDC last update
attributes.value: 2022-12-31T00:58:39Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12255:1_CTRL
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: HEL9217
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: leukemia
attributes.tag: genotype
attributes.value: GFP expression
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: bone marrow
attributes.tag: sample name
attributes.value: E-MTAB-12255:1_CTRL
attributes.tag: stimulus
attributes.value: doxycycline