organism: Homo sapiens
description: Protocols: Primary colonic tissues were obtained from the hospital. Tumoral tissues were processed according to Fujii et al (Fujii et al, 2015) with few modifications. Surgically resected specimens were reduced to a size of 3-5 mm, extensively washed with cold PBS and gentamicin (20microg/ml) and incubated with PBS-EDTA (2,5mM) rocking on a wheel for 1h at 4°C. After PBS-EDTA tissues sample was further washed with cold PBS and tumoral cells were released and collected by centrifuging at 400g 5' 4°C. Not applicable scRNA-seq libraries were prepared according to 10X library preparation kit.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-09-09
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-09-09
attributes.tag: External Id
attributes.value: SAMEA7296449
attributes.tag: INSDC center alias
attributes.value: IFOM, the FIRC Institute of Molecular Oncology
attributes.tag: INSDC center name
attributes.value: IFOM, the FIRC Institute of Molecular Oncology
attributes.tag: INSDC first public
attributes.value: 2022-09-09T00:15:17Z
attributes.tag: INSDC last update
attributes.value: 2022-09-09T00:15:17Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-9283:scSQ_2283
attributes.tag: age
attributes.value: 79
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_type
attributes.value: colorectal cancer cell
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: colorectal cancer
attributes.tag: individual
attributes.value: 76
attributes.tag: organism part
attributes.value: colon
attributes.tag: sample name
attributes.value: E-MTAB-9283:scSQ_2283
attributes.tag: sampling site
attributes.value: neoplasm
attributes.tag: sex
attributes.value: female