organism: Homo sapiens
description: Protocols: Tissues from ileal biopsies Total RNA was extracted from the biopsies using the AllPrep DNA/RNA Mini kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions, after tissue lysis using the FastPrep Lysing Matrix D tubes (MP Biomedicals, Brussels, Belgium) with RLT lysis buffer (Qiagen). The integrity and quantity of RNA was assessed with a 2100 Bioanalyzer (Agilent, Waldbronn, Germany) and a Nanodrop ND-1000 spectrophotometer (Thermo Scientific, Waltham, Massachusetts, USA). Library preparation was performed using the TruSeq Stranded mRNA protocol (Illumina, San Diego, USA) according to the manufacturer's instructions.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-12-30
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-12-30
attributes.tag: External Id
attributes.value: SAMEA8657330
attributes.tag: INSDC center alias
attributes.value: TARGID, KU Leuven
attributes.tag: INSDC center name
attributes.value: TARGID, KU Leuven
attributes.tag: INSDC first public
attributes.value: 2022-12-30T00:23:18Z
attributes.tag: INSDC last update
attributes.value: 2022-12-30T00:23:18Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-10395:GC050141
attributes.tag: age
attributes.value: 49
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: normal
attributes.tag: individual
attributes.value: 12
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: ileum
attributes.tag: sample name
attributes.value: E-MTAB-10395:GC050141
attributes.tag: sampling site
attributes.value: ileal biopsy
attributes.tag: sex
attributes.value: female