organism: Homo sapiens
description: Protocols: HBECs were grown and differentiated at an air-liquid interface (ALI) forming an in vitro 3D-lung model. In short, HBECs were grown and differentiated on 6.5 mm semipermeable transwell inserts (0.4 µm Pore Polyester Membrane Insert, Corning) and after two weeks at air-liquid interface (ALI) the cultures reached full differentiation, which was assessed using light microscopy focusing on epithelial morphology, presence of ciliated cells, and mucus production along with immunofluorescence staining for ciliated cells (acetylated-tubulin, T6793, Sigma) and goblet cells (muc5AC, Ab-1 (45M1), #MS-145-P, ThermoFisher). Primary human bronchial epithelial cells (HBEC) were isolated from proximal airway tissue obtained with informed consent from a patient. At 96 h post infection the mock treated, infected and infected +betamethasone treated HBEC ALI-cultures were washed three times on both sides with PBS. The HBEC ALI-cultures were then lysed and RNA extraction was done using the NucleoSpin RNA II kit (Macherey-Nagel) following the manufacturer's instructions. RNA-seq libraries were prepared using the Smart-seq2 method
identifiers.namespace: BioSample
identifiers.id: SAMEA114458265
attributes.tag: INSDC center name
attributes.value: Laboratory of Molecular Infection Medicine Sweden (MIMS), Umeå university
attributes.tag: Submitter Id
attributes.value: E-MTAB-12368:N702_S508_S59
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_type
attributes.value: lung fibroblast
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: infect
attributes.value: mock
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: lung
attributes.tag: sample name
attributes.value: E-MTAB-12368:N702_S508_S59
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: umuwell
attributes.value: tube3