organism: Homo sapiens
description: Protocols: >1000-fold coverage of mutagenized cells (Brunello library) was infected with Reovirus Type 3 Dearing Strain (MOI=0.1 PFU/cell). When resistant colonies of cells were clearly apparent, the cells were harvested. Uninfected starting populations of mutagenized cells were used as the unselected reference. Total genomic DNA from both virus-resistant and uninfected cells was respectively extracted using QIAamp DNA Mini Kit (Qiagen). The inserted guide RNA sequences were retrieved from the genomic DNA by PCR amplification.
identifiers.namespace: BioSample
identifiers.id: SAMEA11024905
identifiers.namespace: Stanford University
identifiers.id: E-MTAB-11158:Sample 3
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism
attributes.value: Homo sapiens
attributes.tag: disease
attributes.value: chronic myeloid leukemia
attributes.tag: organism part
attributes.value: blood
attributes.tag: cell_line
attributes.value: eHAP
attributes.tag: genotype
attributes.value: lentiCas9-Blast; lentiGuide-Puro
attributes.tag: phenotype
attributes.value: expressing Cas9
attributes.tag: infect
attributes.value: uninfected/unselected control
attributes.tag: genetic modification
attributes.value: CRISPR knockout by Brunello Library (PMID: 26780180)
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-09-10
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-09-10