organism: Mus musculus
description: Protocols: Individual Day 6 EiTiX-embryoids were dissected into four pieces with needles in PBS which were then dissociated with 70μl TrypLE Express Enzyme (Gibco 12604021) at 37ºC for 15 minutes, pipetting up and down every 5 minutes. Dissociation was stopped by adding 500μl FC media supplemented with Y27632 (1:2,000) and DAPI (1:2,000). Day 8 samples were dissociated with 200μl TrypLE Express Enzyme and 800μl media was added to stop the dissociation. The cell suspension was subsequently filtered through a 40μm cell strainer (Merck CLS431750) and further diluted with 2ml FC media. It was then sorted by FACSAria III (BD Biosciences) using index sorting into 384-well plates. Extraction of Single-cell RNA were prepared as previously described (Cheng et al., 2021;Mittnenzweig et al., 2021) following the MARS-seq protocol (Jaitin et al., 2014; Keren-Shaul et al., 2019). Single-cell cDNA libraries were prepared as previously described (Cheng et al., 2021;Mittnenzweig et al., 2021) following the MARS-seq protocol (Jaitin et al., 2014; Keren-Shaul et al., 2019).
identifiers.namespace: BioSample
identifiers.id: SAMEA111329605
identifiers.namespace: PhD student, WIS
identifiers.id: E-MTAB-12140:Sample 268
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism
attributes.value: Mus musculus
attributes.tag: strain
attributes.value: C57BL/6
attributes.tag: developmental stage
attributes.value: embryo stage
attributes.tag: age
attributes.value: NA
attributes.tag: genotype
attributes.value: Day8_EiTiX_embryoid
attributes.tag: embryo
attributes.value: MZG_d8_S4_110322
attributes.tag: organism part
attributes.value: whole organism
attributes.tag: cell_type
attributes.value: Singe cell
attributes.tag: plate
attributes.value: MZG_110322_day8_p2
attributes.tag: experiment
attributes.value: Day8_EiTiX_embryoid
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-09-10
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-09-10