organism: Homo sapiens
description: Protocols: One million SCC25-GFP-Luc cells mixed with; i) one million mouse (MEFs), ii) one million naked mole-rat fibroblasts or, iii) no fibroblasts. 100 μl of cell mixtures were injected subcutaneously to both flanks of 8-week old female immunosuppressed NSG mice. Tumors were collected 28 days after injection SCC25 tumors at day 28 were homogenized in lysis buffer from the Qiagen AllPrep mini kit using Tissue Lyzer II (Qiagen). Total RNA was then isolated using Qiagen AllPrep mini kit (Qiagen) and DNase digested (AM1907, Invitrogen) according to manufactures instructions. RNA concentration was measured using a NanoDrop spectrophotometer(Thermo Fisher): RNA integrity was assessed using RNA ScreenTapes(Agilent Technologies). The RNA integrity number (RIN) was above 8.6 forall samples. 100 ng DNase treated RNA was used to generate ribo-minustreated total RNA stranded libraries using Illumina Stranded Total RNAPrep Ligation with Ribo-Zero Plus kit (Illumina) according to manufacturesinstructions.
identifiers.namespace: BioSample
identifiers.id: SAMEA112239347
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-11-21T00:50:44Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-11-21T00:50:44Z
attributes.tag: External Id
attributes.value: SAMEA112239347
attributes.tag: INSDC center name
attributes.value: Junior Research Group Somatic Evolution and Early Detection German Cancer Research Center (DKFZ), Heidelberg, Germany
attributes.tag: INSDC first public
attributes.value: 2023-11-21T00:50:44Z
attributes.tag: INSDC last update
attributes.value: 2023-11-21T00:50:44Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12455:xg_001-tumor-hl-l-mef-01-x
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: SCC25-GFP-Luc
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: squamous cell carcinoma
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: growth condition
attributes.value: co-injection with mouse fibroblasts
attributes.tag: individual
attributes.value: xg_001
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: tongue
attributes.tag: sample name
attributes.value: E-MTAB-12455:xg_001-tumor-hl-l-mef-01-x
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: male
attributes.tag: xenograft
attributes.value: human squamous carcinoma cells xenografted into nsg mice until neoplasm formation