organism: Homo sapiens
identifiers.namespace: BioSample
identifiers.id: SAMN33997604
identifiers.namespace: GEO
identifiers.id: GSM7133936
attributes.tag: source_name
attributes.value: HEK293
attributes.tag: cell line
attributes.value: HEK293
attributes.tag: cell type
attributes.value: human embryonic kidney cells (HEK293)
attributes.tag: genotype
attributes.value: FTO ORF under CMV promoter. Flp-In T-Rex HEK293 cells which contain a single stably integrated FRT site at a transcriptionally active genomic locus were transfected with pDEST pcDNA5/FRT/TO-FLAG containing FTO.
attributes.tag: treatment
attributes.value: ZBTB48 knockdown was performed (s6568, Thermo Fisher Scientific Inc.) using siZBTB48 for 72 hours in Flp-In T-Rex HEK293 cells, which had FLAG-FTO under the control of doxycycline. Expression was induced for 24 hours prior to crosslinking.
attributes.tag: protocol
attributes.value: iCLIP-seq
attributes.tag: clip antibody
attributes.value: anti-FLAG (Sigma F3165)
attributes.tag: barcoded reverse_transcription_primer
attributes.value: Rt16clip /5Phos/NNTTAANNNAGATCGGAAGAGCGTCGTGgatcCTGAACCGC
attributes.tag: geo_loc_name
attributes.value: missing
attributes.tag: collection_date
attributes.value: missing