organism: human gut metagenome
description: stool sample
identifiers.namespace: BioSample
identifiers.id: SAMEA111438015
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-03-09
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-03-09
attributes.tag: External Id
attributes.value: SAMEA111438015
attributes.tag: INSDC center alias
attributes.value: Max Rubner-Institute
attributes.tag: INSDC center name
attributes.value: Max Rubner-Institute
attributes.tag: INSDC first public
attributes.value: 2023-03-09T08:17:49Z
attributes.tag: INSDC last update
attributes.value: 2023-03-09T08:17:49Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: ernst8029A
attributes.tag: adapters
attributes.value: fwd:AATGATACGGCGACCACCGAGATCTACACNNNNNNNNTATGGTAATTGT::rev:CAAGCAGAAGACGGCATACGAGATNNNNNNNNAAGTCGAGAGTCAGTCAGCC
attributes.tag: amount or size of sample collected
attributes.value: 0.2
attributes.tag: broad-scale environmental context
attributes.value: ENVO_01001033:digestive tract environment
attributes.tag: collection date
attributes.value: 2018-11-19
attributes.tag: environmental medium
attributes.value: ENVO_00002003:fecal material
attributes.tag: geographic location (country and/or sea)
attributes.value: Germany
attributes.tag: geographic location (latitude)
attributes.value: 49.0139822
attributes.tag: geographic location (longitude)
attributes.value: 8.4240071
attributes.tag: host age
attributes.value: 35
attributes.tag: host body product
attributes.value: Feces
attributes.tag: host body-mass index
attributes.value: 25.2
attributes.tag: host sex
attributes.value: male
attributes.tag: human gut environmental package
attributes.value: human-gut
attributes.tag: investigation type
attributes.value: metagenome
attributes.tag: local environmental context
attributes.value: ENVO_2100002:intestine environment
attributes.tag: nucleic acid amplification
attributes.value: PCR
attributes.tag: nucleic acid extraction
attributes.value: Nucleospin Stool Kit (Macherey Nagel)
attributes.tag: pcr conditions
attributes.value: initial denaturation:98degC_2min; 25 cycles: denaturation:98degC_10s; annealing:55degC_30s; extension:72degC_15s; final_extension:72degC_5min
attributes.tag: pcr primers
attributes.value: fwd:GTGYCAGCMGCCGCGGTAA::rev:GGACTACNVGGGTWTCTAAT
attributes.tag: project name
attributes.value: ErNst_study
attributes.tag: sample material processing
attributes.value: freezing
attributes.tag: sample name
attributes.value: ernst8029A
attributes.tag: sample storage temperature
attributes.value: -80
attributes.tag: scientific_name
attributes.value: human gut metagenome
attributes.tag: sequencing method
attributes.value: Illumina MiSeq v3
attributes.tag: target gene
attributes.value: 16S-rRNA
attributes.tag: target subfragment
attributes.value: V4