organism: Arabidopsis thaliana
description: Protocols: Shoots of the seedlings were harvested in liquid nitrogen after 48 h gamma exposure and stored at -80°C until analyses. Seeds of the Landsberg erecta (Ler) accession of Arabidopsis thaliana (L) Heyhn, were surface sterilized for 3 min with a solution of 10 mL of 70% ethanol and 10 ul Tween 20, followed by five times wash in distilled water. Seeds were given a quick rinse in 96% ethanol before air drying on a sterile filter paper inside a sterile hood. All seeds were evenly sown on 1/2 MS medium (Murashige & Skoog 1962; Duchefa Biochemie, Harleem, Netherland) with 0.8% agar (Plant agar, Sigma-Aldrich) in petri dishes of 5 cm diameter. The seeds were then germinated for 6 days at 20°C under a photon flux density of 30 μmol m-2 s-1 at 400-700 nm (TL-D 58W/840 lamps, Phillips) in a 16 h photoperiod. Six days old seedlings were exposed to gamma radiation for 48h at gamma dose rates 1, 10, 40, 100, or 290 mGy/h, using the FIGARO low dose gamma irradiation facility (60Co; 1173.2 and 1332.5 keV γ-rays) at Norwegian University of Life Sciences (Hansen et al. 2019). During the gamma irradiation, the room temperature was set at 20˚C±1°C with a 12 h photoperiod with a photon flux density of 55 μmol m-2 s-1 provided by high pressure metal halide lamps (HPI-T Plus 250W lamps, Phillips). Control samples not exposed to gamma radiation were positioned outside the radiation sector, shielded by lead walls, in the same room and under same light and temperature conditions. Total RNA was extracted using the Masterpure Plant RNA Purification Kit (Epicenter, Madison, WI, USA) following the manufacturer`s instructions except that 0.5 % polyvinylpyrrolidone (PVP), mw 360 000, Sigma Aldrich, Steinheim, Germany) was added to the extraction buffer and that 3 µl beta-mercaptoethanol per sample replaced the 1,4-dithiothreitol (DTT) in the manufacturer`s protocol. RNA quality was assessed using an Agilent 2100 Bioanalyzer with an RNA 144000 NanoKit (Agilent technologies, Palo Alto, CA, USA) and the quantity of isolated RNA was measured by a NanoDrop ND-1000 Spectrophotometer (Thermo Scientific, Wilmington, DE, USA). Strand-specific 20xTruseqTM RNA library preparation kit.
identifiers.namespace: BioSample
identifiers.id: SAMEA10773775
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-01-01
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-01-01
attributes.tag: External Id
attributes.value: SAMEA10773775
attributes.tag: INSDC center alias
attributes.value: NORWEGIAN UNIVERSITY OF LIFE SCIENCES
attributes.tag: INSDC center name
attributes.value: NORWEGIAN UNIVERSITY OF LIFE SCIENCES
attributes.tag: INSDC first public
attributes.value: 2023-01-01T00:27:04Z
attributes.tag: INSDC last update
attributes.value: 2023-01-01T00:27:04Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11120:21-Ara48hGS4-10
attributes.tag: age
attributes.value: 6
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: thale cress
attributes.tag: developmental stage
attributes.value: seedling development stage
attributes.tag: ecotype
attributes.value: Landsberg erecta
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: shoot
attributes.tag: sample name
attributes.value: E-MTAB-11120:21-Ara48hGS4-10