organism: Homo sapiens
description: Protocols: The differentiation protocol of midbrain organoids was adapted. Briefly, we patterned the cells using the Kriks et al. 2011 protocol http://dx.doi.org/10.1038/nature10648 and we matured the neurons using the Jo et al. protocol . Dissociated single cells were washed with PBS three times, and counted and checkedfor viability on a Countess 3FL. 10,000 live single cells per sample were loaded onto the Chromium controller (10X Genomics) for single cell partitioning and barcoding. midbrain organoid dopaminergic differentiation Chromium Next GEM Single Cell 3' Kit v3.1 Chromium Next GEM Single Cell 3' Kit v3.1
identifiers.namespace: BioSample
identifiers.id: SAMEA112151160
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-01-01
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-01-01
attributes.tag: External Id
attributes.value: SAMEA112151160
attributes.tag: INSDC center alias
attributes.value: UNIVERSITY OF CAMBRIDGE
attributes.tag: INSDC center name
attributes.value: UNIVERSITY OF CAMBRIDGE
attributes.tag: INSDC first public
attributes.value: 2023-01-01T00:29:05Z
attributes.tag: INSDC last update
attributes.value: 2023-01-01T00:29:05Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12339:SNCA3x_UNT
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: EDi001-B
attributes.tag: cell_type
attributes.value: dopaminergic neuron
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: Parkinson's disease
attributes.tag: genotype
attributes.value: SNCA-3x overexpression
attributes.tag: growth condition
attributes.value: monoculture
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: floor plate of midbrain
attributes.tag: progenitor cell type
attributes.value: induced pluripotent stem cell
attributes.tag: progenitor organism part
attributes.value: skin
attributes.tag: sample name
attributes.value: E-MTAB-12339:SNCA3x_UNT