organism: Homo sapiens
description: Protocols: The differentiation protocol of midbrain organoids was adapted. Briefly, we patterned the cells using the Kriks et al. 2011 protocol http://dx.doi.org/10.1038/nature10648 and we matured the neurons using the Jo et al. protocol . Dissociated single cells were washed with PBS three times, and counted and checkedfor viability on a Countess 3FL. 10,000 live single cells per sample were loaded onto the Chromium controller (10X Genomics) for single cell partitioning and barcoding. midbrain organoid dopaminergic differentiation no antioxidants + 0.125 uM rotenone treatment for 24 hours Chromium Next GEM Single Cell 3' Kit v3.1 Chromium Next GEM Single Cell 3' Kit v3.1
identifiers.namespace: BioSample
identifiers.id: SAMEA112151163
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-01-01
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-01-01
attributes.tag: External Id
attributes.value: SAMEA112151163
attributes.tag: INSDC center alias
attributes.value: UNIVERSITY OF CAMBRIDGE
attributes.tag: INSDC center name
attributes.value: UNIVERSITY OF CAMBRIDGE
attributes.tag: INSDC first public
attributes.value: 2023-01-01T00:29:05Z
attributes.tag: INSDC last update
attributes.value: 2023-01-01T00:29:05Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12339:SNCA-corr_ROT
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: EDi001-B
attributes.tag: cell_type
attributes.value: dopaminergic neuron
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: Parkinson's disease
attributes.tag: genotype
attributes.value: corrected SNCA expression
attributes.tag: growth condition
attributes.value: monoculture
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: floor plate of midbrain
attributes.tag: progenitor cell type
attributes.value: induced pluripotent stem cell
attributes.tag: progenitor organism part
attributes.value: skin
attributes.tag: sample name
attributes.value: E-MTAB-12339:SNCA-corr_ROT