organism: Homo sapiens
description: To characterize the sensitivity of the RNA export-based population dynamics reporter system, HEK293 cell populations were clonally labeled using genetic barcodes delivered by lentivirus. 10 labeled cells were sorted into a well together with a carrier population of 29,990 HEK293T cells. Culture supernatant was harvested 24 hours after sorting. RNA was extracted from this supernatant and RNA barcodes were reverse transcribed, amplified by PCR, and sequenced. This sample is from well 8 of 8 replicate wells.
identifiers.namespace: BioSample
identifiers.id: SAMN33725952
identifiers.namespace: pda|rfhorns@era
identifiers.id: population_dynamics_sensitivity_well8
attributes.tag: isolate
attributes.value: HEK293T
attributes.tag: age
attributes.value: not applicable
attributes.tag: biomaterial_provider
attributes.value: ATCC CRL-3216 www.atcc.org
attributes.tag: sex
attributes.value: female
attributes.tag: tissue
attributes.value: embryonic kidney
attributes.tag: cell_line
attributes.value: HEK293T (CRL-3216)
attributes.tag: culture_collection
attributes.value: ATCC:CRL-3216
attributes.tag: sample_type
attributes.value: RNA barcode exported by cells in cell culture
attributes.tag: treatment
attributes.value: characterizing sensitivity
attributes.tag: time
attributes.value: 1
attributes.tag: replicate
attributes.value: 8
attributes.tag: BioSampleModel
attributes.value: Human