organism: Saccharomyces cerevisiae
description: Protocols: Cells were proliferated in SC medium at 30°C and allowed to enter stationary phase (SP). Following entry, cells were incubated in for 7 additional days. Cells were collected at 0h, 0.75h and 2.25h by centrifugation and were resuspended in fresh SC medium at 10 fold smaller cell density, followed by incubation at 30°C. Cells were grown in SC medium to reach stationary phase and incubated for 7 additional days, cells were refeed with 10 fold more media to let them exit from starvation, and harvested at 0h, 0.75h and 2.25h post refeeding. Total RNA was purified with standard Phenol: Chloroform extraction. Library construction was done using PAS sequencing protocol (Yoon et.al. 2021, Methods in Enzymology).
identifiers.namespace: BioSample
identifiers.id: SAMEA111263395
identifiers.namespace: Department of Biostatistics, University of Florida
identifiers.id: E-MTAB-12111:W1S1
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism
attributes.value: Saccharomyces cerevisiae
attributes.tag: strain
attributes.value: BY4741, MATa his3D1 leu2D0 met15D0, CaUra3∆::Xrn1WT
attributes.tag: organism part
attributes.value: whole organism
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: growth condition
attributes.value: starvation exit
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-09-10
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-09-10