organism: Heterocephalus glaber
description: Protocols: Naked mole rats (Heterocephalus glaber) or mice (Mus musculus, C56BL6/J) were culled by neck decapitation and full-thickness 4 and 1 mm2 dorsal skin biopsies, respectively, were immediately collected. Naked mole rats (Heterocephalus glaber) or mice (Mus musculus, C56BL6/J) were culled by neck decapitation and full-thickness 4 and 1 mm2 dorsal skin biopsies, respectively, were immediately collected. Single cell cDNA libraries were prepared using the 10X Genomics Chromium Single Cell 3' kit (User Guide Version 3) according to the manufacture's instruction. Quantification of the libraries was carried out using the Qubit dsDNA HS Assay Kit (Life Technologies), and cDNA integrity was assessed using D1000 ScreenTapes (Agilent Technologies).
identifiers.namespace: BioSample
identifiers.id: SAMEA112239056
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-11-21T00:50:43Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-11-21T00:50:43Z
attributes.tag: External Id
attributes.value: SAMEA112239056
attributes.tag: INSDC center name
attributes.value: Division of Regulatory Genomics and Cancer Evolution, German Cancer Research Center (DKFZ), Heidelberg, Germany Junior Research Group Somatic Evolution and Early Detection German Cancer Research Center (DKFZ), Heidelberg, Germany
attributes.tag: INSDC first public
attributes.value: 2023-11-21T00:50:43Z
attributes.tag: INSDC last update
attributes.value: 2023-11-21T00:50:43Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12459:sc-exp_003_skin-5400-3
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: naked mole-rat
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: normal
attributes.tag: environmental history
attributes.value: Essen/Dresden Zoo
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: individual
attributes.value: 5400
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: skin of body
attributes.tag: replicate
attributes.value: sc-exp_003_skin-5400-3
attributes.tag: sample name
attributes.value: E-MTAB-12459:sc-exp_003_skin-5400-3
attributes.tag: scientific_name
attributes.value: Heterocephalus glaber
attributes.tag: sex
attributes.value: male
attributes.tag: treatment
attributes.value: none