organism: Homo sapiens
description: HEK293 cell populations were prepared with puromycin or zeocin resistance and clonally labeled using genetic barcodes delivered by lentivirus. 5,000 cells of each drug-resistant population were sorted together into a well, then cultured for 5 days. Supernatant containing exported RNA was collected at 24 hour intervals. RNA barcodes were reverse transcribed, amplified by PCR, and sequenced. This sample was collected at day 3 of growth without drug selection.
identifiers.namespace: BioSample
identifiers.id: SAMN33725935
identifiers.namespace: pda|rfhorns@era
identifiers.id: population_dynamics_no_selection_day3
attributes.tag: isolate
attributes.value: HEK293T
attributes.tag: age
attributes.value: not applicable
attributes.tag: biomaterial_provider
attributes.value: ATCC CRL-3216 www.atcc.org
attributes.tag: sex
attributes.value: female
attributes.tag: tissue
attributes.value: embryonic kidney
attributes.tag: cell_line
attributes.value: HEK293T (CRL-3216)
attributes.tag: culture_collection
attributes.value: ATCC:CRL-3216
attributes.tag: sample_type
attributes.value: RNA barcode exported by cells in cell culture
attributes.tag: treatment
attributes.value: no selection
attributes.tag: time
attributes.value: 3
attributes.tag: replicate
attributes.value: 1
attributes.tag: BioSampleModel
attributes.value: Human