organism: Mus musculus
description: Protocols: TCR75 cells were FACS sorted from mice into Takara RNA isolation sort buffer (as per Takara instructions)  CD4+CD45.1+ TCR75/Rag-/- T cells (TCR75) were adoptively transferred into CD45.2+ B6 hosts either untransplanted and immunized with B/c donor splenocytes i.p. (UnTx+DST) to induce memory, or prior to transplantation of a B/c heart (HTx) in the presence of anti-CD154 (day 0, day 7, day 14) and DST (day 0) to induce tolerance (Tol). A group of tolerant mice were infected with Lm on d30 post-transplantation (Tol+Lm). CD45.1+ TCR75 cells were harvested from the spleen and lymph nodes on d35-37 post-transplantation in all groups, enumerated and subjected to bulk RNAseq. RNA was isolated from sorted cells using the Takara SMART-Seq v4 Ultra Low Input kit according to manufacturer directions.  RNA libraries were constructed using Nextera XT Library Prep kit and IDP UDI index kit according to manufacturer directions. Libraries were quantified, then equal quantity of library was pooled before sequencing
identifiers.namespace: BioSample
identifiers.id: SAMEA114334938
attributes.tag: INSDC center name
attributes.value: University of Chicago
attributes.tag: Submitter Id
attributes.value: E-MTAB-13333:Listeria58
attributes.tag: age
attributes.value: 24
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_type
attributes.value: TCR75-Tg CD4-pos T cell
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: donor strain
attributes.value: Balb/c
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: individual
attributes.value: 58
attributes.tag: infect
attributes.value: listeria monocytogenes
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: spleen and lymph nodes
attributes.tag: sample name
attributes.value: E-MTAB-13333:Listeria58
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: C57BL6
attributes.tag: transplant
attributes.value: Balb/c cardiac allograft