organism: freshwater metagenome
description: eDNA captured from surface water of a river on a 0.45 micron pore size filter
identifiers.namespace: BioSample
identifiers.id: SAMN33687408
identifiers.namespace: pda|dreynen@uoguelph.ca
identifiers.id: R6-8
attributes.tag: Biological Replicate
attributes.value: 2
attributes.tag: Control Type
attributes.value: NA
attributes.tag: collection_date
attributes.value: 2021-08-05
attributes.tag: depth
attributes.value: 0
attributes.tag: env_broad_scale
attributes.value: terrestrial biome [ENVO:00000446]
attributes.tag: env_local_scale
attributes.value: river [ENVO:00000022]
attributes.tag: env_medium
attributes.value: river water [ENVO:01000599]
attributes.tag: geo_loc_name
attributes.value: Canada: Rockwood, Ontario
attributes.tag: lat_lon
attributes.value: 43.639167 N 80.143333 W
attributes.tag: collection_method
attributes.value: environmental DNA (eDNA) collected by on-site filtration of ~2 L of surface water using a peristaltic pump (OSMOS, Halltech) with a 0.45 micron pore size mixed cellulose ester filter preserved in Longmire's buffer
attributes.tag: conduc
attributes.value: 565 μS/cm
attributes.tag: isolation_source
attributes.value: surface water from a river
attributes.tag: neg_cont_type
attributes.value: deionized water (field control), new unused filter (extraction control), molecular grade water (PCR control)
attributes.tag: ph
attributes.value: 8.22
attributes.tag: pos_cont_type
attributes.value: mock community of fish tissue DNA extracts from Marden Creek, Guelph, Ontario, Canada
attributes.tag: samp_collect_device
attributes.value: OSMOS eDNA Sampler, Halltech (Guelph, Ontario, Canada)
attributes.tag: samp_mat_process
attributes.value: preservation in Longmire's buffer
attributes.tag: samp_size
attributes.value: 2.06 L
attributes.tag: samp_store_dur
attributes.value: ~5 months
attributes.tag: samp_store_loc
attributes.value: freezer
attributes.tag: samp_store_temp
attributes.value: -20 C
attributes.tag: size_frac
attributes.value: 0.45
attributes.tag: temp
attributes.value: 25.3
attributes.tag: turbidity
attributes.value: 283 ppm TSS
attributes.tag: BioSampleModel
attributes.value: MIMARKS.survey
attributes.tag: BioSampleModel
attributes.value: MIGS/MIMS/MIMARKS.water