organism: Homo sapiens
description: Protocols: Samples were collected from cultured primary fibroblasts after exposure to two different X-ray radiation stimuli. Fibroblasts were exposed to two different radiation stimuli using the X-ray irradiator 43855FC-P160 from Faxitron X-Ray LLC (Lincolnshire, IL): 1. An acute dose of radiation (1 pulse of 2 Gy following lysis after 30 minutes). 2. An accumulative dose (4 pulses of 5 Gy, one each 12h, following lysis after 2h from the last dose). Total RNA was extracted from the samples that comprised the study using the QIAzol lysis reagent and purified with RNeasy mini kit (Qiagen). cDNA libraries were constructed from total RNA samples using the TruSeq Stranded Total RNA Prep kit (Illumina).
identifiers.namespace: BioSample
identifiers.id: SAMEA8741344
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-01-01
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-01-01
attributes.tag: External Id
attributes.value: SAMEA8741344
attributes.tag: INSDC center alias
attributes.value: Departamento de Biologia Celular, Fisiologia e Inmunologia, Universidad de Cordoba Instituto Maimonides de Investigacion Biomedica de Cordoba (IMIBIC) Hospital Universitario Reina Sofia
attributes.tag: INSDC center name
attributes.value: Departamento de Biologia Celular, Fisiologia e Inmunologia, Universidad de Cordoba Instituto Maimonides de Investigacion Biomedica de Cordoba (IMIBIC) Hospital Universitario Reina Sofia
attributes.tag: INSDC first public
attributes.value: 2023-01-01T00:26:30Z
attributes.tag: INSDC last update
attributes.value: 2023-01-01T00:26:30Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-10456:Sample 2
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: fibroblast derived cell line
attributes.tag: cell_type
attributes.value: fibroblast
attributes.tag: common name
attributes.value: human
attributes.tag: environmental stress
attributes.value: control
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: skin of body
attributes.tag: sample name
attributes.value: E-MTAB-10456:Sample 2