organism: Ectopleura larynx
description: Protocols: To collect E. larynx, two standard nylon net samples (30 x 50 cm) were submerged at 10 m depth between net pens at the SINTEF ACE research salmon farm site in Vinjefjorden, mid Norway, on 11th of  August 2020. The samples were photographed weekly to monitor growth and were retrieved 23 days later, on 3rd of September. Samples were transferred to the SINTEF SeaLab laboratory in Trondheim in seawater and stored over night at 2°C. Taxonomic identification was carried out the following day based on Schuchert (2010), using a dissecting microscope. Individual hydroid polyps were cleaned of other biofouling organisms (e.g., algae) or associated fauna (e.g., caprellid shrimp) using pure ethanol. Total RNA was extracted from two samples of E. larynx using ZymoBIOMICS RNA Miniprep Kit (Zymo Research), following kit instructions. The RNA output was then reverse transcribed to cDNA using QuantaBio qScript cDNA SuperMix (Quantabio) following manufacturer protocol. Two polyA enriched mRNA libraries were built using TruSeq stranded mRNA kit (Illumina) according to the library prep kit instructions.
identifiers.namespace: BioSample
identifiers.id: SAMEA113987310
attributes.tag: INSDC center name
attributes.value: Center for Biotechnology - Bielefeld University
attributes.tag: Submitter Id
attributes.value: E-MTAB-13150:Sample 1
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: collection date
attributes.value: not collected
attributes.tag: developmental stage
attributes.value: mixed
attributes.tag: environmental history
attributes.value: SINTEF ACE research salmon farm site in Vinjefjorden, mid Norway
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: whole organism
attributes.tag: sample name
attributes.value: E-MTAB-13150:Sample 1
attributes.tag: scientific_name
attributes.value: Ectopleura larynx