title: AZA_rep1
organism: Homo sapiens
description: Protocols: HCT-116 cells were cultured in McCoy medium supplemented with 10% FBS, 100 U/mL penicillin and 100 µg/mL streptomycin. For 5-aza-deoxycytidine (DAC) treatment, cells were plated at a density of 100,000 cells/well in 6-well plates and treated with DAC at a final concentration of 1 μM for a total of 5 days. Fresh medium and drug were added daily for the first 3 days. Total RNA was purified by two successive cycles of TRI Reagent extraction Directional poly(A)+ RNA-Seq libraries were prepared using NEBNext Ultra II Directional RNA Library Prep kit for Illumina