title: Model organism or animal sample from Mus musculus
organism: Mus musculus
description: Approximate 0.5-1g of visceral adipose tissues were harvested from ob/ob mice or WT littermate mice. Then, the tissues were cut into small pieces less than 0.1 cm3 and cultured in DMEM culture medium with exosomes-free 10%FBS for 36h, then refreshed with DMEM for another 36h. A total of 20ml of the supernatant was collected for exosomes isolation.