title: Genome sequencing and assembly of B. Carinata HC20
organism: Brassica carinata
description: Homozygous plants of B. carinata accession HC20 were used for genome sequencing experiments. The HC20 accession was maintained by self-pollination for several generations and was mostly homozygous. For genome sequencing experiments, seedlings were grown in a growth chamber at 8 h light, 25 degree Celsius and 16 h dark, 10 degree Celsius cycle. Leaves were harvested from 15-days-old seedlings and immediately snap-frozen in liquid nitrogen and used for DNA extraction.