title: Transcriptome sequencing from clonal T. brucei selected for monomorphism
organism: Trypanosoma brucei brucei
description: Raw RNA sequencing data from five clones of pleomorphic T. brucei EATRO 1125 AnTat 1.1 90:13. The clones were grown in HMI-9 at 37oC and 5 percent CO2 for a total of 72 days. The HMI-9 media was supplemented with BHI. During the 72-day selection, the percentage of BHI was progressively increased from 2.5 percent to 15 percent. The cell density was counted at each passage using a Beckman Z2 Coulter particle counter and size analyser. High cell density was deliberately maintained to select proliferating cells at high parasite population density. RNA was extracted from triplicate cultures of the 'start' and 'end' from the five clones described above. The cells were grown to a density of between 7.5x10*5 cells/ml and 1x10*6 cells/ml and RNA was then extracted using the Qiagen RNeasy Mini kit, following the manufacturer's instructions. RNA was then sequenced with DNBseq by BGI (Hong Kong).