title: Sample 2
organism: Homo sapiens
description: Protocols: NTERA2/D1 (NT2/D1) cell line was used in the experiment. shRNAs were transduced to NTERA2/D1 cells, targeting RFP as control and targeting SINHCAF as test, and selected by puromycin for 7 days. Cell pellettes are selected. Total RNA was extracted from cell lines using the Trizol reagent. 1.3 ug of total RNA was used for the construction of sequencing libraries. RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacturer's protocols.