title: EV_1
organism: Homo sapiens
description: Protocols: Cells were collected after 24 hours from the induction of OVOL2 expression (by doxycycline). Cells were cultured in RPMI medium supplemented with 10% FBS and 1% P/S. Total RNA was extracted by Maxwell RSC simplyRNA Cells kit (Promega, Madison, Wisconsin, USA) Libraries were obtained starting from 100ng of total RNA following the Illumina Stranded Total RNA preparation Ligation with Ribo-Zero Plus protocol.