title: 250 uM C6-MDB Treated - Primary Rat Hepatocytes
organism: Rattus norvegicus
description: This sample consists of primary rat hepatocytes from male Sprague Dawley rats treated with 250 uM C6-MDB, a c6-modified methylenedioxybenzene xenobiotic. The treatment was designed to study the specific effects of C6-MDB on gene expression and alternative splicing in hepatic cells. This concentration was selected to assess the cellular response and potential toxicological impacts under controlled in vitro conditions. The sample aims to elucidate the biochemical and molecular mechanisms by which C6-MDB influences hepatic metabolism and gene regulation, contributing to broader understanding of xenobiotic metabolism in liver cells. Cells were maintained in standard culture conditions and RNA was extracted immediately post-treatment to preserve integrity for subsequent analysis.