title: Sample 5_GEX
organism: Mus musculus
description: Protocols: 3-, 12- and 18-month-old C57BL/6 male (Janvier) mice were repeatedly immunized every 14 days (5 times) subcutaneously (s.c.) into the flank with 10 mg of human TNFR2 protein (Peprotech, 310-12) and 20 mg MPLA (Sigma, L6895) adjuvant in 150 mL PBS. All samples are from mice immunized with TNFR2 protein. Cells were isolated via facs following the aforementioned experimental conditions 10x genomics 5' protocol was performed as according to manufacturers 10x genomics 5' protocol was performed as according to manufacturers