title: 100 uM CBZ Treated - Primary Rat Hepatocytes
organism: Rattus norvegicus
description: This sample includes primary rat hepatocytes from male Sprague Dawley rats treated with 100 uM Carbamazepine (CBZ), which is known for its role as a mixed inducer of various cytochrome P450 (CYP) isoforms, including CYP2B2. The treatment is part of a broader study designed to assess and compare the effects of different xenobiotics on gene expression and splicing within hepatic cells. By analyzing CBZ alongside various methylenedioxybenzene (MDB) compounds, this research aims to understand the structural and functional relationships affecting CYP enzyme induction and global gene splicing and regulation. This comparative approach helps to elucidate the specific pathways through which different xenobiotics influence liver metabolism and regulation. The hepatocytes were maintained under optimal conditions and RNA was extracted immediately after treatment to ensure integrity for downstream biochemical analyses.