title: WT1
organism: Mus musculus
description: Protocols: Tregs were isolated from mice and cultured in vitro for 3 days with activation using anti-CD3/CD28 beads.  Total RNA was extracted using the Trizol method. Following homogenization with Trizol, chloroform was added, followed by centrifugation. The aqueous phase was used for further isolation of RNA using isopropanol precipitation. Novogene Corporation Inc prepared the RNA-seq 250-300 bp insert cDNA library.