title: Untreated Primary Rat Hepatocytes
organism: Rattus norvegicus
description: This sample is an untreated control of primary rat hepatocytes from male Sprague Dawley rats, used to establish baseline gene expression and splicing profiles for comparison with vehicle and xenobiotic-treated groups. No treatment or vehicle was applied to these cells. The sample serves to provide a reference point for distinguishing the effects of xenobiotics from those of the vehicle controls, including 0.5 uM and 1.0 uM DMSO. Cells were maintained under standard conditions and cryopreserved immediately post-collection to preserve RNA integrity. This untreated control is essential for interpreting the specific impact of vehicle and treatment effects observed in the study's analysis.