title: RUNX1_ChIP_vs_ER_rep2
organism: Homo sapiens
description: Protocols: Cells were sorted to high purity for RUNX1b/Vector (Cherry) and ETV6-RUNX1/ETV6-RUNX1-R139G (GFP) co-expression, expanded in vitro, and approximately 10^8 cells harvested for subsequent ChIP experiments. Cells were crosslinked with 1% formaldehyde and sonicated to yield chromatin of 100-500 bp. ChIP was performed by as previously described (May et al., 2013) using antibodies: RUNX1 (Abcam, ab23980), V5 (Abcam, ab9116). Libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645)