title: 75C
organism: Gallus gallus
description: Protocols: A total of five birds from the MD-susceptible (ADOL 7 2) line were placed into a Horsfall-Bauer (HB) unit at hatch. At twelve days of age, they were sacrificed. After euthanizing the birds, the spleens were aseptically removed, homogenized, filtered to single-cell suspension through 35 micron cell strainers (Falcon, Inc), enriched for mononuclear leukocytes over Histopaque-1077 and transferred the same day on ice for single-cell capture and library preparation of a targeted 3,000 cells/sample. Single cell capture and cDNA library preparation were performed on the 10x Genomics Chromium Single Cell 3' instrument (10x Genomics, Pleasanton, CA) along with the 10x Chromium Single Cell 3' Reagent Kits v2 (Catalog number: PN-120237) according to the manufacturer's recommendations.
attributes.tag: phenotype
attributes.value: Marek's disease virus -susceptible
attributes.tag: strain
attributes.value: USDA Avian Disease Ontology Laboratory line 7_2
attributes.tag: individual
attributes.value: 75C
attributes.tag: organism
attributes.value: Gallus gallus
attributes.tag: organism
attributes.value: Gallus gallus
attributes.tag: scientific_name
attributes.value: Gallus gallus
attributes.tag: common name
attributes.value: chicken
attributes.tag: organism part
attributes.value: spleen
attributes.tag: developmental stage
attributes.value: juvenile stage
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-09-16T00:15:38Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-09-16T00:15:38Z
attributes.tag: cell_type
attributes.value: mononuclear cell
attributes.tag: age
attributes.value: 12
attributes.tag: infect
attributes.value: none
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-09-16
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-09-16