title: PML::RARA_WT-V5_input_ChIP-seq_replicate_2
organism: Mus musculus
description: Bone marrow cells from C57Bl/6 mice were lineage-depleted and retrovirally transduced with murine stem cell virus (MSCV) vectors containing an internal ribosome entry site (IRES) followed by a Thy1.1 cDNA to allow for the purification of transduced cells. These vectors either contained no insert ("empty vector"), or WT PML::RARA cDNA with a V5 epitope tag on C-terminus (PR-V5 or PML::RARA_WT-V5). Two days following the transduction transduced cells were purified with anti-Thy1.1 magnetic bead enrichment, treated with 1 mM diisopropyl fluorophosphate, and anti-V5 ChIP-seq was performed. Genomic DNA from PML::RARA_WT-V5 MSCV transduced cells was saved an input control.
attributes.tag: strain
attributes.value: C57Bl/9
attributes.tag: age
attributes.value: 8 weeks
attributes.tag: collection_date
attributes.value: 2022-01-22
attributes.tag: geo_loc_name
attributes.value: USA
attributes.tag: sex
attributes.value: male
attributes.tag: tissue
attributes.value: Lineage-depleted bone marrow
attributes.tag: genotype
attributes.value: Wild-type
attributes.tag: BioSampleModel
attributes.value: Model organism or animal