title: C10
organism: Mus musculus
description: Protocols: Tumor cells were separated from the C57BL/6 mice on the 10th of tumor growth, Tumor Dissociation Kit (Miltenyi,130-096-730) was used to dissociate the tumor tissues into single cells as the manufacturer's instructions. ACK lysis buffer (Gibco,492-01) was used to remove the red blood cell. Then Dead Cell Removal kit (Miltenyi,130-090-101) was used to remove dead cells by column in MACS Separator, and CD45+ cells were enriched from tumor tissues according to CD45(TIL) MicroBeads (Miltenyi,130-110-618).The cells were resuspended with 1×PBS containing 0.04% BSA, and cell viability was detected with 0.4% trypan blue staining solution and counted with Countess II (Invitrogen) cell counter, and the cell viability was over 90%. The cell suspension was loaded into Chromium microfluidic chips with 30v3 chemistry and barcoded with a 10× Chromium Controller (10X Genomics). mRNA from the barcoded cells was reverse-transcribed into cDNA according to the manufacturer's instructions . Sequencing library was constructed according to the instructions of Chromium Single Cell 3ʹ Reagent kits v3 (10×Genomics).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-06-10
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-06-10
attributes.tag: External Id
attributes.value: SAMEA9147509
attributes.tag: INSDC center alias
attributes.value: Frontier Science Center for Stem Cell Research, Tongji University, Shanghai 200092, China.
attributes.tag: INSDC center name
attributes.value: Frontier Science Center for Stem Cell Research, Tongji University, Shanghai 200092, China.
attributes.tag: INSDC first public
attributes.value: 2023-06-10T00:17:27Z
attributes.tag: INSDC last update
attributes.value: 2023-06-10T00:17:27Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-10610:C10
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: immunophenotype
attributes.value: CD45 positive
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: colon
attributes.tag: sample name
attributes.value: E-MTAB-10610:C10
attributes.tag: sampling site
attributes.value: neoplasm
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: stimulus
attributes.value: injection with MC38-Bap1-Wildtype tumor cells
attributes.tag: strain
attributes.value: C57BL/6