organism: Fusarium sp. TZ1MST22
attributes.tag: strain
attributes.value: TZ1MST22
attributes.tag: ecotype
attributes.value: Tunisia
attributes.tag: collection_date
attributes.value: not provided
attributes.tag: env_broad_scale
attributes.value: oasis
attributes.tag: env_local_scale
attributes.value: oasis Tozeur
attributes.tag: env_medium
attributes.value: plant stem
attributes.tag: geo_loc_name
attributes.value: Tunisia: Tozeur
attributes.tag: host
attributes.value: Medicago sativa
attributes.tag: isol_growth_condt
attributes.value: Symptomatic organs were disinfected in a 3 % sodium hypochlorite solution for 1 min followed by immersion in 70 % ethanol for 30 s, and rinsed in sterile distilled water. Disinfected plant organs were dried on sterile filter paper and placed on potato dextrose agar (PDA) (Pronadisa, Madrid, Spain) medium for 3 days at 25 C ( 1 C) in the dark. The PDA was supplemented with streptomycin sulphate (Bio Basic, Toronto, Canada) at 100 g mL-1. Once the mycelia had developed from infected parts of the organ, the fungal isolates were purified by the hyphal tip method . Stock cultures were maintained on PDA slants at 4 C and subcultured periodically.
attributes.tag: lat_lon
attributes.value: 33.935854 N 8.163181 E
attributes.tag: BioSampleModel
attributes.value: MIGS.eu
attributes.tag: BioSampleModel
attributes.value: MIGS/MIMS/MIMARKS.plant-associated