title: MOLM13 CB 2
organism: Homo sapiens
description: Protocols: MOLM-13 cells were detached from by addition of trypsin. Cells suspensions were quenched with medium and centrifuged at 400 x g for 5 minutes to pellet for subsequent extraction. MOLM-13 cells were cultured in RPMI1640 supplemented with 20% fetal bovine serum and 1% penicillin/streptomycin. Cells were treated with 450 nM CB-5083 (Cayman Chemical 19311) for 48 hours Total RNA was extracted using Trizol (Invitrogen) and purified using Direct-zol RNA Purification Kits (Zymo Research). Library construction was performed by Novogene UK (Cambridge, UK). A minimum of 1 ug (20 ng/Î¼l) of high-quality total RNA (extracted using Trizol, Invitrogen, and purified using Direct-zol RNA Purification Kits, Zymo Research) was supplied for sequencing. Novogene UK constructed cDNA libraries by purification of messenger RNA using poly-T oligo-attached magnetic beads. After fragmentation, the first strand cDNA was synthesized using random hexamer primers followed by the second strand cDNA synthesis. The library was ready after end repair, A-tailing, adapter ligation, size selection, PCR amplification, and purification.
attributes.tag: disease
attributes.value: acute myeloid leukemia
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-04-22
attributes.tag: organism
attributes.value: Homo sapiens
attributes.tag: organism
attributes.value: Homo sapiens
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-04-22
attributes.tag: cell_line
attributes.value: MOLM-13
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: common name
attributes.value: human
attributes.tag: organism part
attributes.value: blood
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-04-22
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-04-22