organism: Gadus macrocephalus
description: Gametes were collected from wild Pacific cod adults (1 female, 3 males) caught off Kodiak Island, AK, USA and transported to Newport, OR. Eggs were fertilized and reared in six treatments (3 temperatures x 2 pH conditions). Post-flexion larvae were sampled after ~2-4 weeks in treatments, preserved as whole-bodies in RNAlater, then homogenized for mRNA isolation.
attributes.tag: strain
attributes.value: not applicable
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: cultivar
attributes.value: not applicable
attributes.tag: ecotype
attributes.value: Gametes collected from adults caught off Kodiak Island, Alaska, spawning population of origin unclear
attributes.tag: collection_date
attributes.value: 2022-05-16
attributes.tag: env_broad_scale
attributes.value: marine biome [ENVO:00000447]
attributes.tag: env_local_scale
attributes.value: marine pelagic [ENVO:01000023]
attributes.tag: env_medium
attributes.value: marine water [ENVO:00001999]
attributes.tag: geo_loc_name
attributes.value: USA: off Kodiak Island, Alaska
attributes.tag: isol_growth_condt
attributes.value: experimental temperature: 6C; experimental pH: ~7.5; larval standard length: 6.272; larval myotome height: 0.315
attributes.tag: lat_lon
attributes.value: 57.988 N 151.934 W
attributes.tag: biotic_relationship
attributes.value: free living
attributes.tag: BioSampleModel
attributes.value: MIGS.eu