title: KO2
organism: Mus musculus
description: Protocols: Tregs were isolated from mice and cultured in vitro for 3 days with activation using anti-CD3/CD28 beads.  Total RNA was extracted using the Trizol method. Following homogenization with Trizol, chloroform was added, followed by centrifugation. The aqueous phase was used for further isolation of RNA using isopropanol precipitation. Novogene Corporation Inc prepared the RNA-seq 250-300 bp insert cDNA library.
attributes.tag: ENA first public
attributes.value: 2024-08-10
attributes.tag: INSDC center name
attributes.value: Johns Hopkins University School of Medicine
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-14288:KO2
attributes.tag: age
attributes.value: 6
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: Ccr6 KO
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: spleen
attributes.tag: sample name
attributes.value: E-MTAB-14288:KO2
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: 5793