title: lead-treated replicate 1
organism: Escherichia coli DH5[alpha]
description: E. coli DH5α was cultivated in LB medium until reaching an OD600 of 0.5 and subsequently mixed with Lead-II-acetate to a final concentration of 75 mM for in vivo lead probing. After incubation for 7 minutes at 37 °C, reactions were stopped with ice-cold EDTA and total RNA was extracted using peqGOLD TriFast® (VWR) according to the manufacturers protocol, including an additional DNase I digestion. From every total RNA sample, two RNA-seq libraries were prepared, utilizing Arabidopsis thaliana RNL and E. coli RtcB to specifically capture 2ʹ,3ʹ-cP and 5ʹ-OH carrying fragments.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-04-19
attributes.tag: organism
attributes.value: Escherichia coli DH5[alpha]
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-04-19
attributes.tag: scientific_name
attributes.value: Escherichia coli DH5[alpha]
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-04-19
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-04-19