title: 113
organism: Mus musculus
description: Protocols: The entire colon was removed from either Hsp60Δ/ΔIEC or Hsp60fl/fl mice weighed and stored in Dulbecco's Modified Eagle Medium (DMEM). After being washed with PBS and minced on ice, tissue specimens were dissociated and enzymatically digested (Multi Tissue Dissociation Kit using gentleMACS™ Dissociator, Miltenyi Biotec). After centrifugation and resuspension in PBS+2% FCS, the cell suspension was strained through a 70-µm strainer, spun down and resuspended in 2% FCS/PBS including RNase inhibitor (New England Biolabs, catalog no. M0314L,1:100). Debris removal solution (Miltenyi Biotec, catalog no. 130-109-398) was used to remove cell debris from the dissociated tissue. Then the dead cell removal kit (Miltenyi, catalog no. 130-090-101) was used to enrich live cells. CD45+ cells were sorted out using the CD45 Microbeads kit (Miltenyi Biotec, catalog no. 130-052-301).  https://cdn.10xgenomics.com/image/upload/v1660261285/support-documents/CG000204_ChromiumNextGEMSingleCell3_v3.1_Rev_D.pdf https://cdn.10xgenomics.com/image/upload/v1660261285/support-documents/CG000204_ChromiumNextGEMSingleCell3_v3.1_Rev_D.pdf
attributes.tag: ENA first public
attributes.value: 2024-07-09
attributes.tag: INSDC center name
attributes.value: German Cancer Research Center (DKFZ), Heidelberg Technical University Munich, School of Medicine
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-14206:113
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_type
attributes.value: CD45 cell
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: house mouse
attributes.tag: genotype
attributes.value: Hsp60Δ/ΔIEC (inducible deletion of the mitochondrial chaperone Hsp60 in intestinal epithelial cells )
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: colon
attributes.tag: sample name
attributes.value: E-MTAB-14206:113
attributes.tag: scientific_name
attributes.value: Mus musculus