title: NC_empty_rep3
organism: Homo sapiens
description: Protocols: Cells were collected using Trypsin‐EDTA (0.025%, Thermo Fisher Scientific). AF22 Neuroepithelial Stem cells (NES) were cultured as adherent cells on cell culture flasks previously coated with 20 μg/ml polyornithine (Sigma) for 1 hour and 1 μg/ml Laminin2020 for 4 hours (Sigma). Cells were cultured on NES culture medium contained DMEM/F12+GlutaMax (Gibco), supplemented with 10 μl/ml N‐2‐supplement (100×, Thermo Fisher Scientific), 10 μl/ ml Penicillin‐Streptomycin (10,000 U/ml, Thermo Fisher Scientific), 1 μl/ml B27‐supplement (50×, Thermo Fisher Scientific), 10 ng/ml of bFGF (Life Technologies) and 10 ng/ ml of FGF (PeproTech). The culture medium was replaced every second day. The NES cells were passaged enzymatically when reaching 100% confluency using Trypsin‐EDTA (0.025%, Thermo Fisher Scientific). NES cells were seeded at a density of 40,000 cells/cm2. Nes cells were seeded at the density of 18750 cell/cm2 and let inside the cell culture incubator to attach for 6 hours. Cells were stimulated with different Jag1Fc nanopatterns for 3 hours. RNA extraction was performed by using RNeasy Micro Kit (Qiagen). Libraries were prepared with TruSeq stranded mRNA (Illumina 20020595) according to manufactures instructions.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-11-23T12:00:05Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-11-23T12:00:05Z
attributes.tag: External Id
attributes.value: SAMEA112193814
attributes.tag: INSDC center name
attributes.value: Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden
attributes.tag: INSDC first public
attributes.value: 2023-11-23T12:00:05Z
attributes.tag: INSDC last update
attributes.value: 2023-11-23T12:00:05Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12439:NC_empty_rep3
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: AF22
attributes.tag: cell_type
attributes.value: fibroblast
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: dermis
attributes.tag: replicate
attributes.value: 3
attributes.tag: sample name
attributes.value: E-MTAB-12439:NC_empty_rep3
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: female