title: HepG2_t15_Xdrop
organism: Homo sapiens
description: Protocols: Cells were harvest using trypsin-EDTA solution (Sigma). Trypsin was subsequently inactivated with a minimum of 3-fold surplus of culture medium. Cells were pelleted and washed once with 1xPBS. Genomic DNA of was extracted using Quick-DNA kit (Zymo Research) according to manufacturer's protocol. The genomic DNA was used to perform Xdrop. In short, the genomic DNA was compartmentalized into droplets and enriched for the target region. The target-enriched DNA molecules were further used for droplet multiple displacement amplification (dMDA). Afterwards, amplified DNA was treated by T7 Endonuclease I, followed by size selection, end-repair, barcoding, and adaptor ligation.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-09-16
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-09-16
attributes.tag: External Id
attributes.value: SAMEA9303455
attributes.tag: INSDC center alias
attributes.value: Department of Microbiology, Tumor, and Cell Biology, Karolinska Institute; Science for Life Laboratory, Sweden
attributes.tag: INSDC center name
attributes.value: Department of Microbiology, Tumor, and Cell Biology, Karolinska Institute; Science for Life Laboratory, Sweden
attributes.tag: INSDC first public
attributes.value: 2022-09-16T00:19:08Z
attributes.tag: INSDC last update
attributes.value: 2022-09-16T00:19:08Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-10652:HepG2_t15_Xdrop
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: HepG2
attributes.tag: cell_type
attributes.value: hepatocyte
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: hepatocellular carcinoma
attributes.tag: genotype
attributes.value: Cas9 deletion of tRNA-Cys-GCA-2-3 and tRNA-Cys-GCA-2-4 gene
attributes.tag: organism part
attributes.value: liver
attributes.tag: sample name
attributes.value: E-MTAB-10652:HepG2_t15_Xdrop