title: 62M
organism: Gallus gallus
description: Protocols: A total of five birds from the MD-resistant (ADOL 6 3) line were placed into a Horsfall-Bauer (HB) unit at hatch. At one week of age, the birds were challenged interperitoneally with 2,000 pfu MDV (JM/102W strain). They were then sacrificed six days post-infection. After euthanizing the birds, the spleens were aseptically removed, homogenized, filtered to single-cell suspension through 35 micron cell strainers (Falcon, Inc), enriched for mononuclear leukocytes over Histopaque-1077 and transferred the same day on ice for single-cell capture and library preparation of a targeted 3,000 cells/sample. Single cell capture and cDNA library preparation were performed on the 10x Genomics Chromium Single Cell 3' instrument (10x Genomics, Pleasanton, CA) along with the 10x Chromium Single Cell 3' Reagent Kits v2 (Catalog number: PN-120237) according to the manufacturer's recommendations.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-09-16
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-09-16
attributes.tag: External Id
attributes.value: SAMEA111326239
attributes.tag: INSDC center alias
attributes.value: Bond Life Sciences Center University of Missouri
attributes.tag: INSDC center name
attributes.value: Bond Life Sciences Center University of Missouri
attributes.tag: INSDC first public
attributes.value: 2022-09-16T00:15:38Z
attributes.tag: INSDC last update
attributes.value: 2022-09-16T00:15:38Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12145:62M
attributes.tag: age
attributes.value: 13
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_type
attributes.value: mononuclear cell
attributes.tag: common name
attributes.value: chicken
attributes.tag: developmental stage
attributes.value: juvenile stage
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: individual
attributes.value: 62M
attributes.tag: infect
attributes.value: Marek's disease virus JM/120W
attributes.tag: organism part
attributes.value: spleen
attributes.tag: phenotype
attributes.value: Marek's disease virus -resistant
attributes.tag: sample name
attributes.value: E-MTAB-12145:62M
attributes.tag: strain
attributes.value: USDA Avian Disease Ontology Laboratory line 6_3