title: Sample 1
organism: Mus musculus
description: Protocols: AT-3 tumor cells were injected into ear pinnae of 8 weeks old C57BL/6 female and male mice (0.3x106 cells in 5μl PBS). Ten days after tumor cell inoculation, mice were divided into 3 groups and either left untreated or were treated with 20×10^6 S. aureus bioparticles or saline vehicle intratumorally, and sacrificed 24h later. Tumors were dissected, placed in 2ml PBS+2%BSA and gently disaggregated through a 100μm cell strainer using a 1ml syringe plunger. Cells were collected, centrifuged and resuspended in 5ml PBS+ 2%BSA. Single-cell suspensions were stained for 30 min at 4°C with Zombie UV™ Fixable Viability kit (Biolegend cat#423107), washed and resuspended in 50ul PBS+1%FBS with fluorophore-conjugated antibodies (FITC-conjugated anti-Ly6G, PeCy7-conjugated anti-CD45, APCCy7-conjugated-anti-CD11b), stained again for 30 min at 4°C, filtered through 100μm cell strainers, and sorted using a BD FACSymphony cell sorter (BD Biosciences) into PBS+2%BSA. The cell preparation time before loading onto the BD Rhapsody was <3h. Cell viability and counting were evaluated with the BD Rhapsody™ Scanner following a standard protocol provided by the manufacturer (BD Biosciences), and samples with viabilities >75% were used for sequencing. Libraries were constructed using the BD Rhapsody WTA Amp Kit (BD Biosciences, cat#633801). Libraries were constructed using the BD Rhapsody WTA Amp Kit (BD Biosciences, cat#633801).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-02-21
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-02-21
attributes.tag: External Id
attributes.value: SAMEA112652173
attributes.tag: INSDC center alias
attributes.value: Garvan Institute of Medical Research
attributes.tag: INSDC center name
attributes.value: Garvan Institute of Medical Research
attributes.tag: INSDC first public
attributes.value: 2023-02-21T10:22:56Z
attributes.tag: INSDC last update
attributes.value: 2023-02-21T10:22:56Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12642:Sample 1
attributes.tag: age
attributes.value: 10
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_type
attributes.value: neutrophil
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: growth condition
attributes.value: AT-3 injected into ear pinnae until neoplasm formation
attributes.tag: immunophenotype
attributes.value: Ly6G+CD11b+
attributes.tag: organism part
attributes.value: pinna
attributes.tag: phenotype
attributes.value: unmanipulated (resting)
attributes.tag: sample name
attributes.value: E-MTAB-12642:Sample 1
attributes.tag: sampling site
attributes.value: neoplasm
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: mixed
attributes.tag: strain
attributes.value: C57BL/6J