title: S2_S6
organism: Fagus sylvatica
description: Protocols: Per plant: harvest of fine roots (< 2mm diameter) in the field, froozen in liquid nitrogen and transport to the lab on dry ice. Storage at -80°C until RNA extraction environmental samples Total RNA was extracted using the protocol of Chang S, Puryear J, Cairney J (1993, A simple and efficient method for isolating RNA from pine trees. Plant Mol Biol Rep 11:113-116). The RNA of four samples was pooled. The cDNA libraries were prepared using NEBNext Ultra RNA Library Prep Kit for Illumina (New England BioLabs, Frankfurt am Main, Germany) according to the manufactorer´s protocol
attributes.tag: sampling site
attributes.value: HEW12
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-04-22
attributes.tag: organism
attributes.value: Fagus sylvatica
attributes.tag: organism
attributes.value: Fagus sylvatica
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-04-22
attributes.tag: growth condition
attributes.value: field study
attributes.tag: scientific_name
attributes.value: Fagus sylvatica
attributes.tag: common name
attributes.value: European beech
attributes.tag: organism part
attributes.value: root
attributes.tag: ecotype
attributes.value: Schorfheide
attributes.tag: environmental history
attributes.value: Hainich
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-04-22
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-04-22