title: 1601_KO
organism: Mus musculus
description: Protocols: cerebellum dissection from experimental animals Transplantation of neural precursor Cells (NPCs, 10 million of cells) or PSB (10ul) intra-cisterna magna, under anaesthesia RNA was extracted by using Purezol (cod.7326890; Bio-rad) following . DNasi I Amplification Grade (cod.AMPD1; Sigma-Aldrich) was used to remove eventual genomic DNA contamination and a second RNA extraction was performed in Purezol. RNA integrity was verified using the RNA 6000 Nano Kit on a Bioanalyzer (Agilent Technologies); all samples had a RIN>9. RNA was quantified using Qubit RNA BR Assay Kit. Libraries were prepared accordingly to the following guides: https://emea.support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/illumina_prep/RNA/illumina-stranded-mrna-reference-guide-1000000124518-02.pdf
attributes.tag: ENA first public
attributes.value: 2024-08-28
attributes.tag: External Id
attributes.value: SAMEA112843718
attributes.tag: INSDC center name
attributes.value: Dept.Biometra, University of Milan
attributes.tag: INSDC last update
attributes.value: 2023-03-28T11:32:31Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12813:1601_KO
attributes.tag: age
attributes.value: 60
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: Mecp2 KO
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: cerebellum
attributes.tag: sample name
attributes.value: E-MTAB-12813:1601_KO
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: CD1