title: OC_475
organism: Homo sapiens
description: Protocols: For each sampling time point, whole blood (9 mL) was collected in cfDNA collection tubes (Dxome, Seongnam, Republic of Korea). Cell-free DNA (cfDNA) was extracted from plasma samples (3–4 mL) using the Magnetic Serum/Plasma Circulating DNA Kit (Dxome, Seongnam, Republic of Korea). The size of the cfDNA was measured using TapeStation 4200 (Agilent Technologies, Santa Clara, CA, USA). cfDNA concentration was measured using a Qubit 3.0 Fluorometer (Thermo Fisher Scientific, Waltham, MA, USA). The resulting DNA was ligated using Illumina adapters and then indexed using unique dual indices for duplex sequencing (Illumina, San Diego, CA, USA). Sequencing libraries were hybridized with customized probes targeting nine ovarian cancer-related genes (TP53, BRCA1, BRCA2, ARID1A, CCNE1, KRAS, MYC, PIK3CA, and PTEN), which are frequently mutated in ovarian cancer.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-11-23T15:00:06Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-11-23T15:00:06Z
attributes.tag: External Id
attributes.value: SAMEA114644271
attributes.tag: INSDC center name
attributes.value: Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, Republic of Korea
attributes.tag: INSDC first public
attributes.value: 2023-11-23T15:00:06Z
attributes.tag: INSDC last update
attributes.value: 2023-11-23T15:00:06Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-13555:OC_475
attributes.tag: age
attributes.value: 59
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_type
attributes.value: circulating tumor cell
attributes.tag: clinical information
attributes.value: individual with suspicious ovarian mass
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: high-grade serous carcinoma
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: individual
attributes.value: OC_475
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: blood
attributes.tag: sample name
attributes.value: E-MTAB-13555:OC_475
attributes.tag: scientific_name
attributes.value: Homo sapiens
attributes.tag: sex
attributes.value: female