title: Empty_vector_anti-V5_ChIP-seq
organism: Mus musculus
description: Bone marrow cells from C57Bl/6 mice were lineage-depleted and retrovirally transduced with murine stem cell virus (MSCV) vectors containing an internal ribosome entry site (IRES) followed by a Thy1.1 cDNA to allow for the purification of transduced cells. These vectors either contained no insert ("empty vector"), or Gata2 cDNA with a V5 epitope tag on C-terminus (Gata2-V5). Four days following the transduction transduced cells were purified with anti-Thy1.1 magnetic bead enrichment, and anti-V5 ChIP-seq was performed. Genomic DNA from Gata2-V5 MSCV transduced cells was saved an input control.
attributes.tag: strain
attributes.value: C57Bl/12
attributes.tag: age
attributes.value: 10 weeks
attributes.tag: collection_date
attributes.value: 2021-08-10
attributes.tag: geo_loc_name
attributes.value: USA
attributes.tag: sex
attributes.value: male
attributes.tag: tissue
attributes.value: Lineage-depleted bone marrow
attributes.tag: genotype
attributes.value: Wild-type
attributes.tag: BioSampleModel
attributes.value: Model organism or animal