title: Saline one-month mouse 501
organism: mouse gut metagenome
description: Saline treatment, mouse 501
attributes.tag: collection_date
attributes.value: 2020-06-18
attributes.tag: env_broad_scale
attributes.value: gut(UBERON:0001007)
attributes.tag: env_local_scale
attributes.value: colon(UBERON:0001155)
attributes.tag: env_medium
attributes.value: feces(UBERON:0001988)
attributes.tag: geo_loc_name
attributes.value: USA:Arkansas,Little Rock (GAZ,00165676)
attributes.tag: host
attributes.value: Mus musculus (C57/BL6)
attributes.tag: lat_lon
attributes.value: 34.7504 N 92.3227 W
attributes.tag: chem_administration
attributes.value: saline (0.9% sodium chloride)
attributes.tag: host_body_habitat
attributes.value: Gastrointestinal
attributes.tag: host_body_product
attributes.value: feces
attributes.tag: host_diet
attributes.value: ad libitum availability to drinking water and standard chow
attributes.tag: host_last_meal
attributes.value: standard chow
attributes.tag: host_life_stage
attributes.value: adult
attributes.tag: host_sex
attributes.value: female
attributes.tag: host_subject_id
attributes.value: 501
attributes.tag: host_taxid
attributes.value: 10090
attributes.tag: perturbation
attributes.value: Intraperitoneal injections on days 1, 8, 15, and 22 with saline (0.9% sodium chloride)
attributes.tag: samp_mat_process
attributes.value: Fecal samples were collected from individual mice and stored at -80C. Fecal pellets were shipped to RTL Genomics (Lubbock, Texas) for DNA extraction and 16S rRNA gene sequencing using their "MiSeq Diversity Assay" targeting the V3V4 hypervariable region, with the primers (357wF: CCTACGGGNGGCWGCAG - 785R: GACTACHVGGGTATCTAATCC).
attributes.tag: samp_store_temp
attributes.value: -80 C
attributes.tag: amplicon_region
attributes.value: V3V4
attributes.tag: forward_primer
attributes.value: CCTACGGGNGGCWGCAG
attributes.tag: reverse_primer
attributes.value: GACTACHVGGGTATCTAATCC
attributes.tag: sequencing_provider
attributes.value: V3V4 16S rRNA gene amplicon sequences were provided by RTL Genomics (Lubbock, Texas).
attributes.tag: cage
attributes.value: 5
attributes.tag: BioSampleModel
attributes.value: MIMARKS.survey
attributes.tag: BioSampleModel
attributes.value: MIGS/MIMS/MIMARKS.host-associated