title: Sample 3
organism: Mus musculus
description: Protocols: 10-week-old C57BL/6J mouse models of type 1 Diabetes mellitus were generated through a single high dose of streptozotocin (STZ, 200 mg/Kg). Mice were fed with a normal chow diet (NCD). C57BL/6J mouse models of type 2 Diabetes mellitus were generated through a 60 kcal% high-fat diet (HFD; D12492, Research Diets) for four weeks, starting at 6 weeks of age. During the fourth week, mice receive daily injections of low-dose STZ (40 mg/Kg) over four consecutive days. C57BL/6J Control mice were fed with a NCD. RNA was extracted from whole heart sections of CTRL, T1DM and T2DM mice starting from OCT embedded tissues Ten sections of 20µm of thickness were collected in 1.5 ml tubes and RNA extraction was performed using RNA Purification kit (Norgen) Libraries were generated using depleted RNA obtained from 1 µg total RNA by TruSeq Sample Preparation RNA Kit (Illumina, Inc., San Diego, CA, USA) according to the manufacturers protocol without further modifications
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-02-20
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-02-20
attributes.tag: External Id
attributes.value: SAMEA112634838
attributes.tag: INSDC center alias
attributes.value: Laboratory of Molecular Medicine and Genomics
attributes.tag: INSDC center name
attributes.value: Laboratory of Molecular Medicine and Genomics
attributes.tag: INSDC first public
attributes.value: 2023-02-20T00:20:06Z
attributes.tag: INSDC last update
attributes.value: 2023-02-20T00:20:06Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12558:Sample 3
attributes.tag: age
attributes.value: 6
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: individual
attributes.value: CTRL3
attributes.tag: organism part
attributes.value: heart
attributes.tag: sample name
attributes.value: E-MTAB-12558:Sample 3
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: strain
attributes.value: C57BL/6J