title: Vic-treated,scRNA-seq
organism: Mus musculus
description: For single-cell sequencing of mouse AOM/DSS colorectal tumors in this study, the single-cell sequencing tissues were obtained from 5 control-treated and 5 6-OHDA-treated mice with colorectal cancer mice.Tumor tissues from each group were pooled together and digested for tissue dissociation. After blocking cell surface antigens with anti-mouse CD16/CD32, epithelial cells and immune cells in the tumor were labeled using FITC-labeled EpCAM and FITC-labeled CD45, respectively. The viability of tumor cells was assessed using 7-AAD to distinguish live and dead cells. Lived EpCAM+ and CD45+ cells were sorted using FACS  for single-cell sequencing.
attributes.tag: strain
attributes.value: C57B/12J
attributes.tag: age
attributes.value: 8 weeks
attributes.tag: dev_stage
attributes.value: not applicable
attributes.tag: collection_date
attributes.value: not applicable
attributes.tag: geo_loc_name
attributes.value: China:beijing
attributes.tag: sex
attributes.value: male
attributes.tag: tissue
attributes.value: colorectal cancer tissue
attributes.tag: cell_type
attributes.value: epithelial cells and immune cells
attributes.tag: sample_type
attributes.value: tissue sample
attributes.tag: treatment
attributes.value: AOM/DSS+Vic
attributes.tag: replicate
attributes.value: not applicable
attributes.tag: BioSampleModel
attributes.value: Model organism or animal