title: Young
organism: Mus musculus
description: Protocols: Liver nuclei (n=4) were prepared from frozen tissue specimens by crushing and dounce homogenising the tissue in 1 ml EZbuffer (SIGMA) (20 strokes with loose and a tight pestle, respectively) and spun 5 min at 300 g. The pellet was incubated on ice for 20 min in EZ-buffer supplemented with DNAseI NEB M0303S (4 units/ml) and 1X DNAseI buffer. Equal volume of EZ-buffer was added and samples were spun 5 min at 500 g and incubated again 10 min on ice in EZ-buffer supplemented with DNAseI NEB M0303S (8 units/ml) and 1X DNAseI buffer. Equal volume of EZ-buffer was added, and samples were spun 5min at 500g, resuspended in NSB (1087.5 µl 1XPBS, 5.5µl 2% BSA, 1.5 µl RNase Inhibitor) and filtered 3 times through a 0.22 µm strainer. For scATAC-seq, 100,000 nuclei were resuspended in 50 µl tagmentation mix. scATAC-seq targeting 4000 cells per sample was performed using a beta version of Chromium Single Cell ATAC Library and Gel Bead kit (10x Genomics, 1000110) according to the manufacturer's instructions.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-02-20
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-02-20
attributes.tag: External Id
attributes.value: SAMEA112634817
attributes.tag: INSDC center alias
attributes.value: Max Planck Institute for Biology of Ageing
attributes.tag: INSDC center name
attributes.value: max planck institute for biology of ageing
attributes.tag: INSDC first public
attributes.value: 2023-02-20T00:20:06Z
attributes.tag: INSDC last update
attributes.value: 2023-02-20T00:20:06Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12706:Young
attributes.tag: age
attributes.value: 3
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_type
attributes.value: hepatocyte
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: individual
attributes.value: pooled
attributes.tag: organism part
attributes.value: liver
attributes.tag: phenotype
attributes.value: young
attributes.tag: sample name
attributes.value: E-MTAB-12706:Young
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: C57BL/6N