title: Sample 2
organism: Mus musculus
description: Protocols: hippocampus dissection from experimental animals Transplantation of neural precursor cells (NPCs, 10 million of cells) or PBS (10 ul) intra-cisterna magna, under anaesthesia RNA was extracted using Purezol (cod.7326890 Biorad). DNasi I amplification Grade (cod.AMPD1, Sigma-Aldrich) was used to remove possible genomic DNA contamination and a second RNA extraction was performed with Purezol. RNA integrity was verified using the RNA 6000 nano Kit on a Bioanalizer (Agilent Technologies). All samples had a RIN>9. RNA was quantified using a Qubit RNA BR Assay Kit. Libraries were prepared according to the following reference guide: /https://emea.support.illumina.com/content/dam/illumina-support/documents/documentation/chemistry_documentation/illumina_prep/RNA/illumina-stranded-mrna-reference-guide-1000000124518-02.pdf
attributes.tag: ENA first public
attributes.value: 2024-08-28
attributes.tag: INSDC center name
attributes.value: University of Pisa
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-14403:Sample 2
attributes.tag: age
attributes.value: 60
attributes.tag: assay name
attributes.value: 1551_HP_WT
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: house mouse
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: hippocampus
attributes.tag: sample name
attributes.value: E-MTAB-14403:Sample 2
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: male
attributes.tag: strain
attributes.value: CD1
attributes.tag: treatment
attributes.value: no treatment