title: mpra_SH-SY5Y_diff_1
organism: Homo sapiens
attributes.tag: source_name
attributes.value: SH-SY5Y
attributes.tag: cell type
attributes.value: cell line
attributes.tag: age
attributes.value: day 6
attributes.tag: cell line
attributes.value: SH-SY5Y
attributes.tag: growth
attributes.value: SH-SY5Y cells (ATCC, CRL-2266) were grown in 1:1 F12 (Lonza, cat. no. 12-615F): EMEM (Thermo Fisher Scientific cat. no. 50-188-268FP). SH-SY5Y cells were differentiated in Neurobasal medium (Thermo Fisher no 21103049) with B27 (Thermo Fisher no 17504044) and Glutamax (Thermo Fisher no 35050061) supplements and 10 µM all-trans-retinoic acid (ATRA) according to the published protocol11. Half-media changes were made every day for 6 days.