title: 20_0268
organism: soil metagenome
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2024-01-23T16:40:28Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2024-01-23T16:40:28Z
attributes.tag: External Id
attributes.value: SAMEA114388868
attributes.tag: INSDC center name
attributes.value: UiT The Arctic University of Norway
attributes.tag: INSDC first public
attributes.value: 2024-01-23T16:40:28Z
attributes.tag: INSDC last update
attributes.value: 2024-01-23T16:40:28Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: 20_0268
attributes.tag: broad-scale environmental context
attributes.value: tundra biome
attributes.tag: collection date
attributes.value: 2018-08-27
attributes.tag: environmental medium
attributes.value: soil
attributes.tag: geographic location (country and/or sea)
attributes.value: Sweden
attributes.tag: geographic location (latitude)
attributes.value: 68.32722
attributes.tag: geographic location (longitude)
attributes.value: 18.84793
attributes.tag: geographic location (region and locality)
attributes.value: Abisko, Norrbottens lan
attributes.tag: host common name
attributes.value: Salix glauca
attributes.tag: local environmental context
attributes.value: heath
attributes.tag: nucleic acid amplification
attributes.value: Clemmensen, K.E., Ihrmark, K., Durling, M.B., Lindahl, B.D., 2016. Sample preparation for fungal community analysis by high-throughput sequencing of barcode amplicons. Methods in Molecular Biology 1399, 61�88. https://doi.org/10.1007/978-1-4939-3369-3_4
attributes.tag: nucleic acid extraction
attributes.value: DNEasy Powersoil Pro Kit https://www.qiagen.com/us/resources/download.aspx?id=3d576814-4f1e-4e26-9c94-57d5dc2bb60a&lang=en
attributes.tag: pcr primers
attributes.value: gITS7 ITS4 ITS4a
attributes.tag: plant body site
attributes.value: root
attributes.tag: project name
attributes.value: PRJEB64296
attributes.tag: sample name
attributes.value: 20_0268
attributes.tag: scientific_name
attributes.value: soil metagenome
attributes.tag: target gene
attributes.value: ITS
attributes.tag: target subfragment
attributes.value: ITS2