title: IL10_mouse_model_gut_bacterial_community
organism: mouse gut metagenome
attributes.tag: host
attributes.value: Mus musculus
attributes.tag: isolation_source
attributes.value: intestines
attributes.tag: collection_date
attributes.value: 2021-11-18
attributes.tag: geo_loc_name
attributes.value: USA: Burlington, Vermont
attributes.tag: lat_lon
attributes.value: 44.4759 N 73.2121 W
attributes.tag: rel_to_oxygen
attributes.value: anaerobe
attributes.tag: samp_collect_device
attributes.value: surgical removal of organ and sampling with sterile tools
attributes.tag: samp_mat_process
attributes.value: After euthanasia, digesta (lumen contents) microbial community samples were collected from the ileum, cecum, proximal colon, and distal colon for PMA-pretreatment to preclude later amplicifation of relic/dead DNA, then samples underwent DNA extraction and community sequencing.
attributes.tag: samp_size
attributes.value: 1 cm2 square surface area
attributes.tag: Mouse ID
attributes.value: 5Bl
attributes.tag: Trial Number
attributes.value: 2
attributes.tag: Sample location
attributes.value: cecum
attributes.tag: Sex
attributes.value: male
attributes.tag: Group
attributes.value: broccoli
attributes.tag: Lipcalin_ng_per_g_feces_day0
attributes.value: 0.6826374988229424
attributes.tag: Lipcalin_ng_per_g_feces_day10
attributes.value: 285.4525469850727
attributes.tag: Lipcalin_ng_per_g_feces_day16
attributes.value: 732.9888966764547
attributes.tag: PMA batch
attributes.value: 4
attributes.tag: PMA date
attributes.value: 2021-11-18T00:00:00
attributes.tag: PMA notes
attributes.value: sample divided in half for extraction and for archiving at -80C
attributes.tag: DNA extraction batch
attributes.value: 2
attributes.tag: DNA extraction date
attributes.value: 2021-12-28T00:00:00
attributes.tag: Nucleic Acid(ng/uL)
attributes.value: 4.722
attributes.tag: A260/A280
attributes.value: 1.857
attributes.tag: A260/A230
attributes.value: 0.252
attributes.tag: Sequencing_batch
attributes.value: 2
attributes.tag: Epithelial_Damage
attributes.value: n/a
attributes.tag: Architectural_Changes
attributes.value: n/a
attributes.tag: Infiltration_of_mononuclear_cells
attributes.value: n/a
attributes.tag: Polymorphonuclear_Cells_in_LP
attributes.value: n/a
attributes.tag: Polymorphonuclear_cells_in_Epithelium
attributes.value: n/a
attributes.tag: Presence_of_Erosion_or_Ulcers
attributes.value: n/a
attributes.tag: BioSampleModel
attributes.value: Metagenome or environmental