organism: Hordeum vulgare
attributes.tag: isolate
attributes.value: CI_16151_Mla6_1_0_hai_NAC
attributes.tag: age
attributes.value: 7 day old
attributes.tag: dev_stage
attributes.value: First leaf
attributes.tag: collection_date
attributes.value: 2022-04-16
attributes.tag: geo_loc_name
attributes.value: USA: Iowa, Ames
attributes.tag: tissue
attributes.value: barley infected first leaf (PO:0007094)
attributes.tag: disease
attributes.value: Bgh isolate 5874 (AVRa6)
attributes.tag: disease_stage
attributes.value: 0 HAI
attributes.tag: genotype
attributes.value: CI 16151 (Resistant progenitor)
attributes.tag: growth_protocol
attributes.value: The CI 16151 (Mla6) progenitor and the mla6-m18982 mutant were maintained via single seed descent. Genotypes were grown in separate trays (19.5 x 27.5-cm) in a climate-controlled greenhouse using a split plot design. Each tray consisted of six rows of 16 seedlings, with rows randomly assigned to one of four harvest timepoints (0, 16, 20, and 32 hai).
attributes.tag: treatment
attributes.value: Barley 1st leaves for the CI 16151 (Mla6) progenitor and the mla6-m18982 mutant were inoculated with Bh isolate 5874 (AVRa6) and collected at four timepoints (0, 16, 20, 32 HAI) and two independent biological replicates. This tissue was used for chromatin preparation using the Diagenode ChIP-seq/ChIP-qPCR Profiling service (Diagenode Cat# G02010000) with the Universal Plant ChIP-seq kit (Diagenode Cat# C01010152).
attributes.tag: BioSampleModel
attributes.value: Plant