organism: Homo sapiens
attributes.tag: isolate
attributes.value: Sequencing Library preparation was carried out in accordance with the manufacturer's instructions. In brief, total genomic DNA was extracted from tissue samples using the Amp Genomic DNA Kit (TIANGEN Biotech, Beijing, China). After shearing the genomic DNA to an average size of 200 bp, 2.5 ng of the fragmented DNA was used to create the sequencing library. End-repair and A-tailing was then perform, followed by the ligation of 1085-bp barcoded sequencing adaptors and treated DNA fragments. PCR was performed subsequently and the its product was purified using the magnetic beads.
attributes.tag: age
attributes.value: NA
attributes.tag: biomaterial_provider
attributes.value: Yifang Dai
attributes.tag: sex
attributes.value: female
attributes.tag: tissue
attributes.value: chorionic villi
attributes.tag: BioSampleModel
attributes.value: Human