title: Pbex16S-24F-Lucie_GGTCAC-L9VDC
organism: biofilm metagenome
attributes.tag: host
attributes.value: not applicable
attributes.tag: isolation_source
attributes.value: Culture biofilm of Cylindrotheca closterium - Tank biofilm
attributes.tag: collection_date
attributes.value: 2023-03-24
attributes.tag: geo_loc_name
attributes.value: France: Cylindrotheca closterium AC170 from Algobank Caen Collection, University of Caen Basse-Normandie, originally isolated from La Tremblade in 1991
attributes.tag: lat_lon
attributes.value: 45.46 N 1.16 W
attributes.tag: samp_mat_process
attributes.value: Cylindrotheca closterium was cultivated as a biofilm using a small rotating experimental culture system under controlled laboratory conditions (controlled temperature of 20°C and light of 80-90 µmol photons.m-2.s-1) with weekly sterile culture medium addition (modified f/2 medium with twice the concentration in nitrogen, phosphate and silicate, using synthetic sea salts Instant Ocean adjusted to reach a salinity of 40 g.L-1). A plastic tank (L35 cm x W25 cm x H15 cm) was used and contained a roller with a length of 30 cm and a diameter of 10 cm, with a rotating bar allowing for tangential velocity ranging from 0.02 to 0.07 m.s-1. A textile fabric was autoclaved and placed on the roller using zip ties. The fabric was moistened on the roller with a sterile culture medium for one day and inoculated by evenly pouring onto the fabric the planktonic culture of C. closterium at 5.7 rotations per minute (rpm) under a light intensity of 90 µmol photons m-2.s-1. The plastic tank was then half-filled with 3.6 L of sterile culture medium, ensuring that ¾ of the roller was exposed to the air while ¼ was immerged in the culture medium.
attributes.tag: BioSampleModel
attributes.value: Metagenome or environmental