title: mpra-AST_2
organism: Homo sapiens
attributes.tag: source_name
attributes.value: primary
attributes.tag: cell type
attributes.value: primary astrocytes
attributes.tag: cell line
attributes.value: primary
attributes.tag: growth
attributes.value: Normal Human Astrocytes (Lonza, CC-2565) were cultured in Lonza Astrocyte Growth Media containing astrocyte basal medium, 7.5% FBS, ascorbic acid, rhEGF, GA-1000 (gentamicin sulfate-amphotericin), insulin, and L-glutamine. Astrocytes were seeded at a 5,000 cells/cm^2 density and maintained up to 70% confluence before splitting at 37C and 5% CO2. Growth medium was changed the day after seeding and every other day thereafter.