title: 1140_cdk6_HA-deltaC_Input
organism: Mus musculus
description: Protocols: Cells expressing CDK6_WT, CDK6_deltaC, as well as CDK6_KO cells were harvested from cell line culture. Cells were crosslinked with DSG (20 minutes, RT) and 1% formaldehyde (10 minutes, RT) and termination of the fixation procedure was performed using glycine. For immunoprecipitation (IP), 70 Âµl Dynabeads Protein G magnetic beads (Invitrogen) were used. The antibody against the HA tag was ab9110 (Abcam).  Input DNA or DNA enriched through IP was subjected to sequencing library preparation using the NEBNext Ultra II chemistry (New England Biolabs)