title: 2021-W44 Kalmar Amplified SARS-CoV-2 RNA from wastewater
organism: wastewater metagenome
description: Untreated wastewater was collected in Kalmar at Kalmarsundsverket on 2021-11-01 over 24 hours using a flow compensated sampler. Viral genomic material was concentrated and extracted by the direct capture method using the Maxwell RSC Enviro Total Nucleic Acid Kit (Promega Corporation, Madison, WI, USA, Cat # AS1831) according to the manufacturer's protocol (Promega, # TM663 Rev 4/22) at SEEC-SLU. Sequencing libraries were made using Ion AmpliSeq SARS-CoV-2 Insight Research Assay (Thermo Fisher Scientific, Waltham, MA, USA, Cat # A51306) and the SuperScript™ VILO™ cDNA Synthesis Kit (Thermo Fisher, # 11754050) according to the manufacturer's protocols “Reverse transcribe RNA with the SuperScript™ VILO™ cDNA Synthesis Kit” (Thermo Fisher, # MAN0019277, Rev B.0, pp. 3-4) and “Prepare libraries on the Ion Chef™ Instrument” (Thermo Fisher, # MAN0024915 Rev B.0, Ch 4, pp. 42-43) at the National Genomic Infrastructure (NGI) at SciLifeLab in Uppsala, Sweden. Sequencing was performed on the Ion S5 XL System (Thermo Fisher, # A27214) multiplexing using Torrent Suite Software version 5.16.1 configured according to the manufacturer's protocol (Thermo Fisher, # MAN0024915 Rev B.0, Ch 5) at NGI.