title: wt_kv_4h_2
organism: Arabidopsis thaliana
description: 22 degree, short-day light, 75% humidity. Genotype: wt=Col-0. Replicate: 1st biological replicate. Treatment: Infiltration leaves with OD600=0.2 of Pf0-1:AvrRps4(KRVY135-138AAAA mutant) in 10mM MgCl2. Sampling: 4 hours post infiltration. Fresh nuclei were extracted from mature leaves from plants which are 5-week old and sorted with flow cytometry (fluorescence-activated nuclei sorting), and tagmented with Tn5 from an Illumina Nextera kit. ATACseq library was amplified with custom-design and 9nt-space dual-index primers. After multiplexed with other samples are sequenced with four flow cells of Nextseq 500/550 high output kit (75 cycles), and demultiplexed with Illumina dcl2fastq.