title: S21
organism: Phleum pratense
description: Protocols: Field trials at Tromsø and Vesterålen were established in 2017 using the seed from the market and the breeding company Graminor. Four year old field surviving plants collected in late summer of 2020 were freeze tested along with the 4 week old plants raised from the seedlots which were used to establish the field trials. All plants were first cold acclimated under ambient light conditions, first for two weeks at 9°C, followed by 10 days at 6°C and finally three weeks at 2°C under 12h light ( 55 PPFD ). The freezing tests were carried out as described in Dalmannsdottir et al. (2017). Plants were placed in a freezer and sampled when frozen to pre-determined temperatures, i.e., -12, -15, -18, -21, -24 and -27°C. The sampled plant material was crushed with morter and pistel and the grinded material is used for RNA extraction Does not apply RNA extraction was carried out using Qiagen RNeasy Plant Mini kit The libraries were prepared according to the TruSeq RNA Sample Prep Kit v2 (Illumina, San Diego, CA, USA)