title: dsGFP-injected Hyposoter didymator calyx cells at pupal stage 3
organism: Hyposoter didymator
description: Calyx cells dissected from dsGFP-injected Hyposoter didymator females at pupal stage 3  for pupae with pigmented thorax and abdomen (~5 day-old pupae), using three biological replicas (each corresponding to 30 to 50 calyx). Wasps were injected with a non-specific dsRNA homologous to the green fluorescent protein (GFP) gene. Treated pupae were kept in an incubator until adult emergence, which occurred approximately 5 days after injection.