title: Metagenome of Heliopora coerulea
organism: coral metagenome
description: Coral tissue was collected and identified by divers in Bonaparte Archipelago, Kimberely, Western Australia. Total RNA was extracted using the RNeasy Plus Mini Kit (Qiagen, Hilden, Germany) as previously described in (Geoghegan et al. 2021). These libraries were constructed using the Truseq Total RNA Library Preparation Protocol (Illumina). Host ribosomal RNA (rRNA) was depleted with the Ribo-Zero Plus Kit (Illumina) and paired-end sequencing (150 bp) was carried out on the NovaSeq 500 platform (Illlumina). Library construction and metatranscriptomic sequencing were performed by the Australian Genome Research Facility (AGRF).