title: setikv_mk_1
organism: Arabidopsis thaliana
description: 22 degree, short-day light, 75% humidity. Genotype: setikv= SETI with inducible mutant AvrRps4 (KRVY135-138AAAA) (Ngou et al 2019 JXB). Treatment: mk=mock treatment, infiltrated with 0.1% DMSO (in water), harvested at 3.5 hours post infiltration (hpi). Replicate: 1st biological replicate (1/9). Fresh nuclei were extracted from mature leaves from plants which are 5-week old and sorted with flow cytometry (fluorescence-activated nuclei sorting), and tagmented with Tn5 from an Illumina Nextera kit. ATACseq library was amplified with custom-design and 9nt-space dual-index primers. After multiplexed with other samples are sequenced with four flow cells of Nextseq 500/550 high output kit (75 cycles), and demultiplexed with Illumina dcl2fastq.