title: Metagenome or environmental sample from Batch fermentation reactor
organism: fermentation metagenome
description: The aim of this research was to evaluate the optimum conditions for hydrogen production through the co-fermentation of waste from the sugar-alcohol industry, sugarcane bagasse, filter cake and vinasse. The tests were carried out in batches using Rotational Central Composite Design statistical methods to evaluate the addition of FC and vinasse with enzymatically pre-treated sugarcane bagasse. Granular upflow anaerobic sludge thermally pre-treated and Clostridium butyricum were used as inoculum. The hydrogen obtained was produced by the acidocenigenic. Analysis of the microbial community using 16S rRNA sequencing identified bacteria similar to Clostridium, Caproiciproducens, Anaerotruncus, Cellulosilyticum and Lachnospiraceae. Potential hydrolysis genes were inferred, in which enzymes from the endoglucanase, xylanase and arabinofuranosidase classes were identified. Genes related to enzymes that degrade lignin, such as peroxidases, were inferred mainly in the sample from the optimized reactor