organism: plankton metagenome
description: One L. monocytogenes colony was inoculated in 1:2 diluted TSB and grown at 37°C for eight hours (shaking 100 rpm) and adapted at 10°C for 16 hours without shaking. On the inoculation day, this culture was adjusted to an OD600 of 0.1 in 1:10 diluted TSB two hours (10°C, 100 rpm) before addition to the multi-species biofilm (mean cell densities of 8.3 log CFU/ml).