title: L_IL2_WT_15
organism: Mus musculus
description: Protocols: Small tumors (60-80mm3) and large tumors (200-300mm3) with or without the indicated IL-2 treatment (treated for 3 days) were extracted for RNAseq analysis. Total RNA was isolated from each sample using the RNA mini kit (Qiagen, Germany). RNA quality was examined by gel electrophoresis and with Qubit (Thermo, Waltham, MA, USA). For RNA-sequencing, 1Î¼g of total RNA was used for library construction. Sequencing libraries were generated using VAHTSTM Total RNA-seq (H/M/R) Library Prep Kit for Illumina.