organism: biofilm metagenome
description: The stainless-steel slides with Pseudomonas, Brochothrix and Carnobacterium in equal numbers were incubated at 10°C for 24 h with shaking, resulting in a multi-species biofilm. The medium was changed to fresh medium for another two and another four hours, respectively. In total the biofilms had an age of 30 hours. Eight slides were pooled to obtain enough biomass.