organism: biofilm metagenome
description: The stainless-steel slides with Pseudomonas, Brochothrix and Carnobacterium in equal numbers were incubated at 10°C for 24 h with shaking, resulting in a multi-species biofilm. The Listeria inoculum (previously prepared) was added for two hours. Afterwards the medium was changed to fresh medium for another four hours. In total the biofilms had an age of 30 hours, of which 6 hours Listeria were present. Eight slides were pooled to obtain enough biomass.