title: pES6_granu_no_IGF2
organism: Homo sapiens
description: Protocols: The creation of CSES7 cell line is described in Lavon et al. (2008) Derivation of euploid human embryonic stem cells from aneuploid embryos. Stem Cells 26: 1874-1882. CSES10 in Narwani et al. (2010) Human embryonic stem cells from aneuploid blastocysts identified by pre-implantation genetic screening. In Vitro Cell Dev Biol Anim. 46: 309-316. The PES6 and SWAPS4 cell lines are described in Paull et al. (2013) Nuclear genome transfer in human oocytes eliminates mitochondrial DNA variants. Nature: 493 632-637. Cells washed and trypsinized Total RNA was isolated using RNeasy Mini Kit (QIAGEN) RNA sequencing libraries were generated using TruSeq RNA Library Prep Kit (Illumina) according to the manufacturerâ€™s protocol and sequenced using Illumina NextSeq 500 with 75 bp single end reads