title: setigh_un_3
organism: Arabidopsis thaliana
description: 22 degree, short-day light, 75% humidity. Genotype: setigh= SETI with wild-type inducible AvrRps4 (Ngou et al 2019 JXB) crossed into sard1 cbp60g double mutant. Treatment: un=no treatment/untreated. Replicate: 1st biological replicate (1/9). Fresh nuclei were extracted from mature leaves from plants which are 5-week old and sorted with flow cytometry (fluorescence-activated nuclei sorting), and tagmented with Tn5 from an Illumina Nextera kit. ATACseq library was amplified with custom-design and 9nt-space dual-index primers. After multiplexed with other samples are sequenced with four flow cells of Nextseq 500/550 high output kit (75 cycles), and demultiplexed with Illumina dcl2fastq.