organism: Ectocarpus sp. 5 AP-2014
attributes.tag: Alternative strain name
attributes.value: Ec sil Jap16 a-1 m
attributes.tag: Culture conditions
attributes.value: Air-bubbled 10 L carboy cultures in Provasoli-enriched seawater with double strength Provasoli micronutrients plus antibiotics. Grown under 16:8h white light and at 13C.
attributes.tag: DNA or RNA extracted
attributes.value: RNA
attributes.tag: DNA or RNA quality (ratio 260/280)
attributes.value: 2,09
attributes.tag: DNA or RNA sample code
attributes.value: RNA_SXS69
attributes.tag: ENA first public
attributes.value: 2024-01-10
attributes.tag: Extraction date
attributes.value: 03/07/17
attributes.tag: Extraction method
attributes.value: Qiagen RNeasy kit with RLC buffer
attributes.tag: Harvest date
attributes.value: 13/07/16
attributes.tag: INSDC center name
attributes.value: Genoscope CEA
attributes.tag: INSDC status
attributes.value: public
attributes.tag: RNA RIN value
attributes.value: 7,7
attributes.tag: Submitter Id
attributes.value: RNA_SXS69
attributes.tag: additional details
attributes.value: Ec12m and Ec13f are probably brother and sister, derived from a heterozygous field sporophyte. Publication: a brother of this strain (Jap16 a11m) was used in Muller & Kawai, 1991. Sampled in October 1989.
attributes.tag: collected_by
attributes.value: Dieter Muller
attributes.tag: collection date
attributes.value: missing: data agreement established pre-2023
attributes.tag: culture_collection
attributes.value: RCC7100
attributes.tag: generation
attributes.value: partheno-sporophyte
attributes.tag: geographic location (country and/or sea)
attributes.value: Japan
attributes.tag: geographic location (latitude)
attributes.value: 43.05
attributes.tag: geographic location (longitude)
attributes.value: 144.033
attributes.tag: identification by
attributes.value: Dieter Muller
attributes.tag: identification method
attributes.value: Morphology, genome sequenced
attributes.tag: ploidy
attributes.value: haploid
attributes.tag: ratio 260/230
attributes.value: 2,42
attributes.tag: sample alias
attributes.value: RNA_SXS69
attributes.tag: sample description
attributes.value: RNA extracted from Ectocarpus species 5 strain Ec12m for RNA-seq-based genome annotation.
attributes.tag: sample name
attributes.value: RNA_SXS69
attributes.tag: sampling ecosystem
attributes.value: coastal
attributes.tag: sampling sea
attributes.value: Pacific Ocean
attributes.tag: sampling site
attributes.value: Akkeshi, Hokkaido, Japan
attributes.tag: sampling water type
attributes.value: marine
attributes.tag: scientific_name
attributes.value: Ectocarpus sp. 5 AP-2014
attributes.tag: sequencing centre sample code
attributes.value: CBQ_AI
attributes.tag: sex
attributes.value: male
attributes.tag: sex determination method
attributes.value: genetic cross, genome sequencing
attributes.tag: strain
attributes.value: Ec12m
attributes.tag: tissue type
attributes.value: whole thallus