organism: Laminarionema elsbetiae
attributes.tag: Alternative strain name
attributes.value: KU-0356
attributes.tag: Culture conditions
attributes.value: Air-bubbled 10 L carboy cultures in Provasoli-enriched seawater with double strength Provasoli micronutrients plus antibiotics. Grown under 16:8h white light and at 13C.
attributes.tag: DNA or RNA extracted
attributes.value: RNA
attributes.tag: DNA or RNA quality (ratio 260/280)
attributes.value: 2,16
attributes.tag: DNA or RNA sample code
attributes.value: RNA_PHAEO207
attributes.tag: ENA first public
attributes.value: 2024-01-10
attributes.tag: Extraction date
attributes.value: 06/07/17
attributes.tag: Extraction method
attributes.value: Qiagen RNeasy kit with RLC buffer
attributes.tag: Harvest date
attributes.value: 12/08/16
attributes.tag: INSDC center name
attributes.value: Genoscope CEA
attributes.tag: INSDC status
attributes.value: public
attributes.tag: RNA RIN value
attributes.value: 6.40 ( same profile as previous extraction, multiple bands but it should be the normal profile)
attributes.tag: Submitter Id
attributes.value: RNA_PHAEO207
attributes.tag: additional details
attributes.value: Original gametophyte isolated from the field. Publication: Peters, A.F. & Ellertsdottir, E. 1996 Nova Hedwigia 62, 341-349. Sampled in 1994.
attributes.tag: collected_by
attributes.value: Elin Ellertsdottir (student of Akira Peters)
attributes.tag: collection date
attributes.value: missing: data agreement established pre-2023
attributes.tag: culture_collection
attributes.value: RCC7120
attributes.tag: generation
attributes.value: sporophyte
attributes.tag: geographic location (country and/or sea)
attributes.value: Germany
attributes.tag: geographic location (latitude)
attributes.value: 54
attributes.tag: geographic location (longitude)
attributes.value: 7
attributes.tag: identification by
attributes.value: Elin Ellertsdottir/Akira Peters
attributes.tag: identification method
attributes.value: Morphology, genome sequenced
attributes.tag: ploidy
attributes.value: haploid
attributes.tag: ratio 260/230
attributes.value: 2,61
attributes.tag: sample alias
attributes.value: RNA_PHAEO207
attributes.tag: sample description
attributes.value: RNA extracted from Laminarionema elsbetiae strain ELsaHSoW15 for RNA-seq-based genome annotation.
attributes.tag: sample name
attributes.value: RNA_PHAEO207
attributes.tag: sampling ecosystem
attributes.value: coastal
attributes.tag: sampling sea
attributes.value: North Sea
attributes.tag: sampling site
attributes.value: Helgoland, Germany
attributes.tag: sampling water type
attributes.value: marine
attributes.tag: scientific_name
attributes.value: Laminarionema elsbetiae
attributes.tag: sequencing centre sample code
attributes.value: BSI_AQ
attributes.tag: sex
attributes.value: na
attributes.tag: sex determination method
attributes.value: na
attributes.tag: strain
attributes.value: ELsaHSoW15
attributes.tag: tissue type
attributes.value: whole thallus