organism: Halopteris paniculata
attributes.tag: Alternative strain name
attributes.value: KU-1118
attributes.tag: Culture conditions
attributes.value: Air-bubbled 10L bidon containing natural sea water implemented with 0.01% Provasoli enrichment plus antibiotics. Grown under standard light and temperature conditions. Before harvesting, individuals from each Petri dish were transplanted into freshly prepared culture medium in a new Petri dish and cryotubes for maintenance and further harvesting. Harvesting was carried out as follow: the medium from the Petri dish was discarded. All partheno-sporophytes were collected using a plastic squeegee, rapidly dried on clean absorbent paper, put in a 96 well plate containing a metal ball and frozen in liquid nitrogen. Samples were kept at -80C before DNA extraction.
attributes.tag: DNA or RNA extracted
attributes.value: DNA
attributes.tag: DNA or RNA quality (ratio 260/280)
attributes.value: 2,00
attributes.tag: ENA first public
attributes.value: 2024-01-10
attributes.tag: Extraction date
attributes.value: 19/05/17
attributes.tag: Extraction method
attributes.value: OmniPrep elution in TE buffer
attributes.tag: INSDC center name
attributes.value: Genoscope CEA
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: GPO-105
attributes.tag: additional details
attributes.value: Derived from a spore from a unilocular sporangium of a field sporophyte, therefore probably a gametophyte.
attributes.tag: collected_by
attributes.value: Dieter Muller
attributes.tag: collection date
attributes.value: 1981-09-06
attributes.tag: culture_collection
attributes.value: RCC7133
attributes.tag: generation
attributes.value: nd
attributes.tag: geographic location (country and/or sea)
attributes.value: Australia
attributes.tag: geographic location (latitude)
attributes.value: -38.2833
attributes.tag: geographic location (longitude)
attributes.value: 144
attributes.tag: identification by
attributes.value: Dieter Muller
attributes.tag: identification method
attributes.value: Morphology, genome sequenced
attributes.tag: ratio 260/230
attributes.value: 2,66
attributes.tag: sample alias
attributes.value: GPO-105
attributes.tag: sample description
attributes.value: DNA extracted from Halopteris paniculata strain Hal grac a UBK for genome sequencing.
attributes.tag: sample name
attributes.value: GPO-105
attributes.tag: sampling ecosystem
attributes.value: coastal
attributes.tag: sampling sea
attributes.value: Indian Ocean
attributes.tag: sampling site
attributes.value: Pt. Lonsdale, Victoria, Australia
attributes.tag: sampling water type
attributes.value: marine
attributes.tag: scientific_name
attributes.value: Stypocaulon paniculatum
attributes.tag: sex
attributes.value: nd
attributes.tag: strain
attributes.value: Hal grac a UBK
attributes.tag: tissue type
attributes.value: whole thallus