organism: soil metagenome
attributes.tag: collection_date
attributes.value: 2021-11-13
attributes.tag: depth
attributes.value: 0-3 cm
attributes.tag: elev
attributes.value: 275m
attributes.tag: env_broad_scale
attributes.value: woodland area[ENVO:00000109]
attributes.tag: env_local_scale
attributes.value: woodland area[ENVO:00000109]
attributes.tag: geo_loc_name
attributes.value: USA:Wisconsin
attributes.tag: lat_lon
attributes.value: 43.088 N 89.442 W
attributes.tag: annual_precpt
attributes.value: 928 mm
attributes.tag: annual_temp
attributes.value: 7.5 C
attributes.tag: collection_method
attributes.value: soil core
attributes.tag: cur_land_use
attributes.value: hardwoods (e.g. oak,hickory,elm,aspen)
attributes.tag: cur_vegetation
attributes.value: Acer saccharum with Tilia americana and Prunus serotina
attributes.tag: drainage_class
attributes.value: well
attributes.tag: fao_class
attributes.value: Luvisols
attributes.tag: local_class
attributes.value: Whalan silt loam
attributes.tag: local_class_meth
attributes.value: USDA soil classification
attributes.tag: ph
attributes.value: 5.92
attributes.tag: ph_meth
attributes.value: 1:1 CaCl2
attributes.tag: pool_dna_extracts
attributes.value: not pooled
attributes.tag: rel_to_oxygen
attributes.value: aerobe
attributes.tag: samp_collect_device
attributes.value: lined metal core, 7.9 cm dia
attributes.tag: samp_mat_process
attributes.value: 4 C until sieving to 2mm
attributes.tag: samp_size
attributes.value: 150 cm3
attributes.tag: samp_vol_we_dna_ext
attributes.value: 250 mg
attributes.tag: sieving
attributes.value: 53 um
attributes.tag: size_frac
attributes.value: 53-250 um
attributes.tag: soil_horizon
attributes.value: A horizon
attributes.tag: soil_text_measure
attributes.value: silt loam
attributes.tag: soil_type
attributes.value: Whalan silt loam
attributes.tag: soil_type_meth
attributes.value: USDA soil classification
attributes.tag: store_cond
attributes.value: after sieving/aggregate size fractionation, subsamples for DNA extraction were stored at -80 C for up to 3 months prior to DNA extraction
attributes.tag: tot_n_meth
attributes.value: flash combustion
attributes.tag: tot_nitro
attributes.value: 0.0051019001
attributes.tag: tot_nitro_cont_meth
attributes.value: flash combustion
attributes.tag: tot_org_c_meth
attributes.value: flash combustion
attributes.tag: tot_org_carb
attributes.value: 0.07237364292
attributes.tag: site
attributes.value: Lakeshore Nature Preserve, Madison, WI, USA
attributes.tag: treatment
attributes.value: HighAmynthas
attributes.tag: soil_fraction
attributes.value: free microaggregate fraction
attributes.tag: plot
attributes.value: 44
attributes.tag: core
attributes.value: 5
attributes.tag: Fwd_Primer_Barcode
attributes.value: TCGACGAG
attributes.tag: Rev_Primer_Barcode
attributes.value: CTACGCAG
attributes.tag: seq_meth
attributes.value: Illumina MiSeq 2x250 PE
attributes.tag: env_material
attributes.value: soil
attributes.tag: nucl_acid_ext
attributes.value: Qiagen DNeasy PowerLyzer PowerSoil DNA extraction kit
attributes.tag: target_gene
attributes.value: 16S rRNA
attributes.tag: target_subfragment
attributes.value: V4
attributes.tag: pcr_cond
attributes.value: initial denaturation:98_2; denaturation:98_0.167; annealing:58_0.25; elongation:72_0.167; final elongation:72_2; total cycles: 30
attributes.tag: BioSampleModel
attributes.value: MIMS.me
attributes.tag: BioSampleModel
attributes.value: MIGS/MIMS/MIMARKS.soil