organism: Sphaerotrichia firma
attributes.tag: Culture conditions
attributes.value: 140 mm Petri dish cultures in Provasoli-enriched seawater. Grown under 12:12h white light at 13C. No antibiotics.
attributes.tag: DNA or RNA extracted
attributes.value: RNA
attributes.tag: DNA or RNA quality (ratio 260/280)
attributes.value: 1,95
attributes.tag: DNA or RNA sample code
attributes.value: RNA_Sfir13m-2
attributes.tag: ENA first public
attributes.value: 2024-01-10
attributes.tag: Extraction date
attributes.value: 08/08/18
attributes.tag: Extraction method
attributes.value: Qiagen RNeasy kit with RLC buffer
attributes.tag: INSDC center name
attributes.value: Genoscope CEA
attributes.tag: INSDC status
attributes.value: public
attributes.tag: RNA RIN value
attributes.value: 8,1
attributes.tag: Submitter Id
attributes.value: RNA_Sfir13m-3
attributes.tag: additional details
attributes.value: Derived from a heterozygous, field-isolated sporophyte collected by Hiroshi Kawai on 12-07-1988 at Oshoro, Hokkaido. Publications: Peters, A.F., Kawai, H. & Novaczek, I. 1993. Intraspecific sterility barrier confirms that introduction of Sphaerotrichia divaricata (Phaeophyceae, Chordariales) into the Mediterranean was from Japan. Proceedings of the 14th International Seaweed Symposium. Hydrobiologia 260/261: 31-36. Note that classification of this Sphaerotrichia entity in S. firma was only made later (Kim, S.-H., Peters, A.F. & Kawai, H. 2003. Taxonomic revision of Sphaerotrichia divaricata (Ectocarpales, Phaeophyceae), with a reappraisal of S. firma from the north-west Pacific. Phycologia 42:183-192.). Initially it was included in Sphaerotrichia divaricata.
attributes.tag: collected_by
attributes.value: Hiroshi Kawai
attributes.tag: collection date
attributes.value: 1998-07-12
attributes.tag: culture_collection
attributes.value: RCC7267
attributes.tag: generation
attributes.value: gametophyte
attributes.tag: geographic location (country and/or sea)
attributes.value: Japan
attributes.tag: geographic location (latitude)
attributes.value: 43.2
attributes.tag: geographic location (longitude)
attributes.value: 140.867
attributes.tag: identification by
attributes.value: Hiroshi Kawai
attributes.tag: identification method
attributes.value: Morphology, genome sequenced
attributes.tag: ploidy
attributes.value: haploid
attributes.tag: ratio 260/230
attributes.value: 1,88
attributes.tag: sample alias
attributes.value: RNA_Sfir13m-3
attributes.tag: sample description
attributes.value: RNA extracted from Sphaerotrichia firma strain SfirJ13m for analysis of differential gene expression..
attributes.tag: sample name
attributes.value: RNA_Sfir13m-3
attributes.tag: sampling ecosystem
attributes.value: coastal
attributes.tag: sampling sea
attributes.value: Pacific Ocean
attributes.tag: sampling site
attributes.value: Oshoro, Hokkaido, Japan
attributes.tag: sampling water type
attributes.value: marine
attributes.tag: scientific_name
attributes.value: Sphaerotrichia firma
attributes.tag: sequencing centre sample code
attributes.value: CIT_AH
attributes.tag: sex
attributes.value: male
attributes.tag: sex determination method
attributes.value: genetic cross, genome sequencing
attributes.tag: strain
attributes.value: SfirJ13m
attributes.tag: tissue type
attributes.value: whole thallus