organism: biofilm metagenome
attributes.tag: INSDC center name
attributes.value: NETHERLANDS INSTITUTE OF ECOLOGY
attributes.tag: Submitter Id
attributes.value: SBR1_day105_16S
attributes.tag: amount or size of sample collected
attributes.value: 0.015
attributes.tag: broad-scale environmental context
attributes.value: Photobioreactor
attributes.tag: collection date
attributes.value: 2019-12-19
attributes.tag: environmental medium
attributes.value: Water
attributes.tag: geographic location (country and/or sea)
attributes.value: Netherlands
attributes.tag: geographic location (latitude)
attributes.value: 51.98699968
attributes.tag: geographic location (longitude)
attributes.value: 5.67116169
attributes.tag: geographic location (region and locality)
attributes.value: The Netherlands Institute of Ecology (NIOO-KNAW), Wageningen, Gelderland
attributes.tag: local environmental context
attributes.value: Granulated biomass, photogranule
attributes.tag: nucleic acid amplification
attributes.value: PCR
attributes.tag: nucleic acid extraction
attributes.value: Qiagen DNAeasy Power Soil Pro
attributes.tag: number of replicons
attributes.value: 3
attributes.tag: oxygenation status of sample
attributes.value: aerobic
attributes.tag: pH
attributes.value: 6.8
attributes.tag: pcr primers
attributes.value: 341F-805R
attributes.tag: project name
attributes.value: LotusHR
attributes.tag: reactor type
attributes.value: Photobioreactor operated in sequencing batch mode
attributes.tag: sample material processing
attributes.value: 15mL of harvested photogranules were homogenised by a glass/Teflon tissue grinder, aliquoted into five 2 mL Eppendor tubes, centrifuged at 14.870 rcf for 10 min and the supernatant discarded, the cell pellets were immediately frozen at -80 �C until further processing.
attributes.tag: sample name
attributes.value: SBR1_day105_16S
attributes.tag: sample volume or weight for DNA extraction
attributes.value: 0.2
attributes.tag: scientific_name
attributes.value: biofilm metagenome
attributes.tag: sequencing method
attributes.value: Illumnia MiSeq
attributes.tag: sludge retention time
attributes.value: 7
attributes.tag: target gene
attributes.value: 16S rRNA gene
attributes.tag: wastewater type
attributes.value: Synthetic wastewater