organism: uncultured Tenericutes bacterium
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-01-25
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-01-25
attributes.tag: External Id
attributes.value: SAMEA112229491
attributes.tag: INSDC center alias
attributes.value: EMG
attributes.tag: INSDC center name
attributes.value: EMG
attributes.tag: INSDC first public
attributes.value: 2023-01-25T00:20:14Z
attributes.tag: INSDC last update
attributes.value: 2023-01-25T00:20:14Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: SRR15604608_bin.9_metawrap_v1.3_MAG
attributes.tag: assembly quality
attributes.value: Many fragments with little to no review of assembly other than reporting of standard assembly statistics
attributes.tag: assembly software
attributes.value: not provided
attributes.tag: binning parameters
attributes.value: MaxBin2, MetaBat2, Concoct with default parameter of the metaWRAP pipeline. Bin refinement module used from metaWRAP with default parameters.
attributes.tag: binning software
attributes.value: metawrap v1.3
attributes.tag: broad-scale environmental context
attributes.value: mouse digestive system
attributes.tag: broker name
attributes.value: EMG broker account, EMBL-EBI
attributes.tag: collection date
attributes.value: 2019-11-18
attributes.tag: completeness score
attributes.value: 80.39
attributes.tag: completeness software
attributes.value: CheckM
attributes.tag: contamination score
attributes.value: 0.0
attributes.tag: environmental medium
attributes.value: feces
attributes.tag: geographic location (country and/or sea)
attributes.value: China
attributes.tag: geographic location (latitude)
attributes.value: 40.51
attributes.tag: geographic location (longitude)
attributes.value: 110.171
attributes.tag: investigation type
attributes.value: metagenome-assembled genome
attributes.tag: isolation_source
attributes.value: mouse gut metagenome
attributes.tag: local environmental context
attributes.value: digestive tube
attributes.tag: metagenomic source
attributes.value: mouse gut metagenome
attributes.tag: project name
attributes.value: To evaluate the role of probiotics in restoring the antibiotic-destroyed gut microbiota and efficacy of ICB therapies, we studied the effect of Lactobacillus rhamnosus Probio-M9 combined with anti-PD-1 on mouse colon cancer and the dynamic changes of gut microbiome by metagenome analyses. The results showed that the combination of Probio-M9 significantly improved tumor inhibition compared with ICB therapy alone in antibiotics treated mice, which could be attribute to the upregulated microbial diversity and increased abundance of beneficial bacteria such as Bifidobacterium pseudolongum, Parabacteroides distasonis and some species of Bacteroides after Probio-M9 supplemented. These changes of structure and composition in gut microbiota caused the alternation of microbiome function, of which the sugar degradation, vitamin and amino acid synthesis pathways were significantly enriched. Collectively, this study indicated that Probio-M9 enhanced the host&apos;s immunotherapeutic response by promoting the colonization of beneficial bacteria and improving intestinal function.
attributes.tag: sample derived from
attributes.value: SAMN20968513
attributes.tag: sample name
attributes.value: SRR15604608_bin.9_metawrap_v1.3_MAG
attributes.tag: sequencing method
attributes.value: Illumina HiSeq 2500
attributes.tag: taxonomic classification
attributes.value: The taxonomy of this metagenome-assembled genome was originally computed with GTDBtk, which assigned the following taxonomic annotation: d__Bacteria;p__Tenericutes;c__;o__;f__;g__;s__
attributes.tag: taxonomic identity marker
attributes.value: multi-marker approach