organism: Glycine max
attributes.tag: isolate
attributes.value: Room temperature treatment of recipient plants at 25 Celsius
attributes.tag: cultivar
attributes.value: P3
attributes.tag: ecotype
attributes.value: not collected
attributes.tag: age
attributes.value: 9 days of culture
attributes.tag: dev_stage
attributes.value: germinating seed stage
attributes.tag: geo_loc_name
attributes.value: not collected
attributes.tag: tissue
attributes.value: radicles
attributes.tag: biomaterial_provider
attributes.value: Northeast Agricultural University, Harbin, China
attributes.tag: cell_line
attributes.value: not collected
attributes.tag: cell_type
attributes.value: not collected
attributes.tag: collected_by
attributes.value: not collected
attributes.tag: collection_date
attributes.value: not collected
attributes.tag: culture_collection
attributes.value: not collected
attributes.tag: disease
attributes.value: not collected
attributes.tag: disease_stage
attributes.value: not collected
attributes.tag: genotype
attributes.value: not collected
attributes.tag: growth_protocol
attributes.value: Peanut, transgenic soybean, and WT seeds in the same growth state were selected. The seeds were disinfected with 70% (v/w) ethanol for 1 min and soaked in 5% (w/v) sodium hypochlorite solution for 15 min, after which the seeds were rinsed 5 to 6 times with sterile water for 5 to 6 times. Soak completely sterilized soybean seeds in 25 C sterile water in a dark growth chamber for 12 h, and peanut seeds need to be soaked for 24 h. For germination stage, The three transgenic strains and WT were seeded in vermiculite and then transferred into a chamber at lower temperature of 6 C, and, we performed RNA-seq analysis using line1-1 and WT, The radicles of transgenic and WT plants treated for 9 d in the cold chamber were used as samples for RNA-seq.
attributes.tag: height_or_length
attributes.value: not collected
attributes.tag: isolation_source
attributes.value: not collected
attributes.tag: lat_lon
attributes.value: 45.75 N 126 E
attributes.tag: phenotype
attributes.value: not collected
attributes.tag: population
attributes.value: not collected
attributes.tag: sample_type
attributes.value: not collected
attributes.tag: sex
attributes.value: not collected
attributes.tag: specimen_voucher
attributes.value: not collected
attributes.tag: temp
attributes.value: 25
attributes.tag: treatment
attributes.value: 25
attributes.tag: BioSampleModel
attributes.value: Plant