title: Soybean overexpression of the aldehyde dehydrogenase AhALDH3H1 from Arachis hypogaea under water for 28d biological replicate 3
organism: Glycine max
description: Soybean overexpression of the aldehyde dehydrogenase AhALDH3H1 from Arachis hypogaea under water for 28d biological replicate 3
attributes.tag: isolate
attributes.value: Soybean overexpression of the aldehyde dehydrogenase AhALDH3H1 from Arachis hypogaea under water for 28d biological replicate 3
attributes.tag: cultivar
attributes.value: P3
attributes.tag: ecotype
attributes.value: not collected
attributes.tag: age
attributes.value: 35 days of culture
attributes.tag: dev_stage
attributes.value: seedling stage
attributes.tag: geo_loc_name
attributes.value: not collected
attributes.tag: tissue
attributes.value: roots
attributes.tag: biomaterial_provider
attributes.value: Northeast Agricultural University, Harbin, China
attributes.tag: cell_line
attributes.value: not collected
attributes.tag: cell_type
attributes.value: not collected
attributes.tag: collected_by
attributes.value: not collected
attributes.tag: collection_date
attributes.value: not collected
attributes.tag: culture_collection
attributes.value: not collected
attributes.tag: disease
attributes.value: not collected
attributes.tag: disease_stage
attributes.value: not collected
attributes.tag: genotype
attributes.value: not collected
attributes.tag: growth_protocol
attributes.value: The saline-alkali solution with a pH value of 8.9 was prepared as follows; Every 1 L solution contained 2.925 g NaCl, 5.3 g Na2CO3, 37.8 g NaHCO3, and 63.9 g Na2SO4, in a 1: 1: 9: 9 ratio. The original solution was then diluted to 12.5 L and used for saline-alkali treatment. On Sep 1, 2022, we planted six pots of each transgenic line, including L1-1, L2-1, L3-1, L4-1, and WT in a mixture of soil and vermiculite at a 1:4 ratio for both saline-alkali and water treatments. Each pot contained 10 seeds and three biological replicates for each group. Watering with tap water removed unhealthy plants over the next week, and five healthy and identical individuals were saved for further study. On Sep14, 2022, two weeks after planting, the treatment group was watered with a saline-alkaline solution whose pH value was 8.9, as opposed to the control treated with tap water. After 7 d, 14 d, 21 d, and 28 d after treatment, the physiological index was tested and measured.
attributes.tag: height_or_length
attributes.value: not collected
attributes.tag: isolation_source
attributes.value: not collected
attributes.tag: lat_lon
attributes.value: 45.75 N 126 E
attributes.tag: phenotype
attributes.value: not collected
attributes.tag: population
attributes.value: not collected
attributes.tag: sample_type
attributes.value: not collected
attributes.tag: sex
attributes.value: not collected
attributes.tag: specimen_voucher
attributes.value: not collected
attributes.tag: temp
attributes.value: 25
attributes.tag: treatment
attributes.value: water 28d
attributes.tag: biological_replicate
attributes.value: biological replicate 3
attributes.tag: BioSampleModel
attributes.value: Plant