title: Control
organism: Streptomyces coelicolor A3(2)
description: Protocols: S. coelicolor A3(2) was grown on MR5 plates for 24 h, then the substrate mycelia were harvested from agar by gentle scraping. Total RNA of S. coelicolor A3(2) was isolated from cells (approximately 100 mg wet weight) with Sepasol-RNA I super G reagent (Nacalai Tesque, Inc.). After treatment with RNase-free recombinant DNase I (TaKaRa Bio, Inc., Shiga, Japan), each sample was further purified using a High Pure RNA Isolation Kit (Roche Life Science, Penzberg, Germany). High-purity total RNA samples were sent to Bioengineering Lab. Co., Ltd. (Kanagawa, Japan) for the rRNA depletion using riboPOOLS (siTOOLs Biotech, Germany), and library construction using the RNA Directional Library Prep Set (MGI Tech, China).
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-12-10T05:00:06Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-12-10T05:00:06Z
attributes.tag: External Id
attributes.value: SAMEA112954219
attributes.tag: INSDC center name
attributes.value: Department of Microbiology, Faculty of Science, Chulalongkorn University
attributes.tag: INSDC first public
attributes.value: 2023-12-10T05:00:06Z
attributes.tag: INSDC last update
attributes.value: 2023-12-10T05:00:06Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12876:Control
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: genotype
attributes.value: SCP1+ SCP2+
attributes.tag: growth condition
attributes.value: modified R5 agar plate
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: haustorium
attributes.tag: sample name
attributes.value: E-MTAB-12876:Control
attributes.tag: scientific_name
attributes.value: Streptomyces coelicolor A3(2)
attributes.tag: strain
attributes.value: 1147