title: Ad5-N_m1_lung
organism: Severe acute respiratory syndrome coronavirus 2
description: K18-hACE2 mice were intramuscularly vaccinated with adenovirus type-5 (Ad5) vector vaccines that encoded either the SARS-CoV-2 S open reading frame (Ad5-S) or the N open reading frame (Ad5-N) from SARS-CoV-2/human/USA/USA-WA1/2020. Mice were vaccinated with either 1x10^9 PFU of Ad5-S, Ad5-N, both, or with PBS as a control (n = 5 mice per condition). After three weeks, mice were challenged intranasally with 5x10^4 plaque forming units (PFU) of SARS-CoV-2/human/USA/USA-WA1/2020 and euthanized at 5-days post infection. Whole lung and brain tissue was dissected and RNA extracted for viral whole genome sequencing.
attributes.tag: collected_by
attributes.value: Laboratory of Justin Richner at the University of Illinois at Chicago
attributes.tag: collection_date
attributes.value: 2021-02-26
attributes.tag: geo_loc_name
attributes.value: USA: Illinois, Chicago
attributes.tag: host
attributes.value: Homo sapiens
attributes.tag: host_disease
attributes.value: COVID-19
attributes.tag: isolate
attributes.value: SARS-CoV-2/human/USA/USA-WA1/2020
attributes.tag: isolation_source
attributes.value: Obtained from BEI Resources (NR-52281)
attributes.tag: collection_method
attributes.value: Whole tissue dissection
attributes.tag: date_of_sars_cov_2_vaccination
attributes.value: Mice were vaccinated 3 weeks prior to SARS-CoV-2 challenge
attributes.tag: exposure_event
attributes.value: K18-hACE2 (B6.Cg-Tg(K18-ACE2)2Prlmn/J) (Jax Strain # 034860) transgenic mice were challenged intranasally with 5*10^4 plaque-forming units (PFU) of SARS-CoV-2/human/USA/USA-WA1/2020 and tissue was harvested at 5 days post-infection.
attributes.tag: host_anatomical_part
attributes.value: lung
attributes.tag: passage_method
attributes.value: Virus was propagated on Vero-E6 cells (ATCC) for one passage prior to use.
attributes.tag: passage_number
attributes.value: 1
attributes.tag: prior_sars_cov_2_vaccination
attributes.value: yes - completed
attributes.tag: sars_cov_2_diag_gene_name_1
attributes.value: N (orf9)
attributes.tag: sars_cov_2_diag_pcr_ct_value_1
attributes.value: 13.05
attributes.tag: sequenced_by
attributes.value: Center for Pathogen Genomics and Microbial Evolution (CPGME) at Northwestern University, Chicago, Illinois
attributes.tag: vaccine_received
attributes.value: Mice were immunized intramuscularly (50 μL per quadriceps) with an Ad5 vector expressing SARS-CoV-2 nucleocapsid protein (Ad5-N) diluted in sterile PBS, at 10^9 PFU per mouse.
attributes.tag: BioSampleModel
attributes.value: SARS-CoV-2: clinical or host-associated