title: M53T0
organism: gut metagenome
description: Protocols: Fecal samples were collected at baseline, after 12 weeks and 6 months of lifestyle intervention in tubes filled with DNA stabilization buffer (CANVAX Biotech). For 16S rRNA sequencing, the total microbial DNA was extracted using the QIAamp PowerFecal DNA Kit (Qiagen) following the manufacturer's protocol. The Illumina 16S Metagenomic Sequencing Library Preparation for high-throughput sequencing was performed as follows: 12.5 ng of each DNA extract was employed for the amplification of the V3-V4 hypervariable regions of the bacterial 16S ribosomal RNA (rRNA) gene, using the universal primers previously published by Klindworth et al. with Illumina adapters.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2023-11-28T00:25:28Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-11-28T00:25:28Z
attributes.tag: External Id
attributes.value: SAMEA112322340
attributes.tag: INSDC center name
attributes.value: Cancer Stem Cells Unit, ISBReMIT, IRCSS Casa Sollievo della Sofferenza, Opera di San Pio da Pietrelcina
attributes.tag: INSDC first public
attributes.value: 2023-11-28T00:25:28Z
attributes.tag: INSDC last update
attributes.value: 2023-11-28T00:25:28Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12486:M53T0
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: clinical history
attributes.value: Homo sapiens hospitalised patient
attributes.tag: disease
attributes.value: breast cancer
attributes.tag: organism part
attributes.value: feces
attributes.tag: sample name
attributes.value: E-MTAB-12486:M53T0
attributes.tag: scientific_name
attributes.value: gut metagenome