organism: metagenome
description: Short Rotation, Crop Sorghum, Rhizosphere, Replicate IV
attributes.tag: collection_date
attributes.value: 2019
attributes.tag: depth
attributes.value: 0-15 cm
attributes.tag: elev
attributes.value: 57 m
attributes.tag: env_broad_scale
attributes.value: Mineral
attributes.tag: env_local_scale
attributes.value: Soil
attributes.tag: env_medium
attributes.value: Rhizosphere
attributes.tag: organism_type
attributes.value: Bacteria
attributes.tag: geo_loc_name
attributes.value: Uruguay
attributes.tag: lat_lon
attributes.value: 33.15544 S 54.29281 W
attributes.tag: sequencing_platform
attributes.value: Illumina MiSeq
attributes.tag: dna_extraction_method
attributes.value: FastDNA SPIN kit for soil
attributes.tag: dna_extraction_amount
attributes.value: 0.5g
attributes.tag: annual_season_precpt
attributes.value: Annual mean: 1379 ± 58 mm (no seasonality within the year)
attributes.tag: annual_season_temp
attributes.value: Annual Mean maximum: 23.0 ± 0.1°C, annual mean minimum: 11.3 ± 0.6 °C (January (warmest): 23.0 ± 0.5°C/ July (coldest): 10.9 ± 0.9 °C)
attributes.tag: crop_rotation
attributes.value: Trifolium pratense L./Avena sativa L./Sorghum bicolor L./ Triticum aestivum L./Glycine max L.
attributes.tag: cur_land_use
attributes.value: small grains
attributes.tag: cur_vegetation
attributes.value: sorghum
attributes.tag: drainage_class
attributes.value: poorly
attributes.tag: fao_class
attributes.value: Typic Argiudolls
attributes.tag: horizon
attributes.value: A horizon
attributes.tag: link_climate_info
attributes.value: http://www.inia.uy/gras/Clima/Banco-datos-agroclimatico
attributes.tag: previous_land_use
attributes.value: Natural grasslands (before 1995)
attributes.tag: samp_collect_device
attributes.value: soil core auger (2 cm diameter)
attributes.tag: samp_mat_process
attributes.value: 15–20 soil core samples per replicate were randomly collected between plants with a soil core auger (2 cm diameter) at 0–15 cm depth, mixed and homogenized by sieving with 5 mm mesh size (roots and stones were removed) immediately after sampling. For microbial analysis, samples were stored at −20◦C until total community DNA (TC-DNA) extraction.
attributes.tag: sieving
attributes.value: Sieving with 5 mm mesh size
attributes.tag: store_cond
attributes.value: stored at -20°C, immediate extraction
attributes.tag: texture
attributes.value: 39% sand, 39% silt, 22% clay
attributes.tag: Replicate
attributes.value: IV
attributes.tag: BioSampleModel
attributes.value: MIMARKS.survey
attributes.tag: BioSampleModel
attributes.value: MIGS/MIMS/MIMARKS.soil