title: Closterium sp. Mating group I-E from vegetative and mating cultures
organism: Closterium sp. mating group I-E
description: For the vegetative cultures, mt+ (NIES-67) and mt- (NIES-68) cells were separately harvested at 6 (start time of light illumination is 0) and 18 h (IsoSeq 1). For the sexually-differentiated cells, vegetatively growing cells of the mt+ and mt- were separately collected, washed three times with MI medium, and incubated separately in MI medium (3.6E5 cells/ 72 mL in 300-mL Erlenmeyer flasks) under continuous light for 4 and 24 h (IsoSeq 2 and IsoSeq 3, respectively). For the mating-induced cells, cells of both mating types, which had been separately cultured in MI medium (at 3.6E5/ 72 mL in 300-mL Erlenmeyer flasks) for 24 h, were mixed and co-incubated (at 3.6E5 each/ 72 mL in 300-mL Erlenmeyer flasks) for 4 and 8 h (IsoSeq 4), 16 and 24 (IsoSeq 5), and 48 and 72 (IsoSeq 6).
attributes.tag: sample_name
attributes.value: Iso-seq sequence of Closterium sp. mating group I-E
attributes.tag: biomaterial_provider
attributes.value: National Institute for Environmental Studies
attributes.tag: collected_by
attributes.value: Terunobu Ichimura
attributes.tag: culture_collection
attributes.value: NIES:67,68
attributes.tag: env_biome
attributes.value: freshwater
attributes.tag: note
attributes.value: mating group I-E (NIES-67 and NIES-68)