title: flg22 rep3
organism: Arabidopsis thaliana
description: Protocols: Polyamine, flg22 and mock (water) treatments were performed in three biological replicates by leaf- infiltration of 5-week-old wild-type (Col-0) plants. Infiltrated leaves were collected and frozen in liquid nitrogen at 24 h of treatment for total RNA extraction. Seeds of the different genotypes were sown on a soil mixture containing peat moss (40%), vermiculite (50%) and perlite (10%), and stratified in the dark at 4°C for 2-3 days. Plants were grown at 20-22℃ under 12-hour light/ 12-hour dark photoperiod cycles. Leaf-infiltration with 1-ml needless syringe Total RNA was extracted using TriZol (Thermo Fisher) and further purified using RNeasy kit (Qiagen) according to manufacturer's instructions. Libraries were prepared using the MGIEasy RNA Library Prep kit (MGI Tech) according to manufacturer's instructions
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-10-21
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2023-01-01
attributes.tag: External Id
attributes.value: SAMEA110128799
attributes.tag: INSDC center alias
attributes.value: Departament de Biologia, Sanitat i Medi Ambient. Facultat de Farmacia i Ciencies de l'Alimentacio. Seccio de Fisiologia Vegetal. Universitat de Barcelona. Barcelona, Spain
attributes.tag: INSDC center name
attributes.value: Departament de Biologia, Sanitat i Medi Ambient. Facultat de Farmacia i Ciencies de l'Alimentacio. Seccio de Fisiologia Vegetal. Universitat de Barcelona. Barcelona, Spain
attributes.tag: INSDC first public
attributes.value: 2022-10-21T07:00:03Z
attributes.tag: INSDC last update
attributes.value: 2023-01-01T00:28:10Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-11820:flg22 rep3
attributes.tag: age
attributes.value: 5
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: thale cress
attributes.tag: developmental stage
attributes.value: sporophyte vegetative stage
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: organism part
attributes.value: rosette leaf
attributes.tag: sample name
attributes.value: E-MTAB-11820:flg22 rep3
attributes.tag: ecotype
attributes.value: Col-0