title: HA15M01_od-01
organism: Mus musculus
description: Protocols: To generate single cell suspensions, freshly isolated whole tissues of ovary, oviduct and tissue pieces from uterus were treated by enzymatic digestion. All tissues were initially incubated separately in 2 ml Eppendorf tubes containing 0,25% trypsin in PBS and digested for 30 min at 37°C with gentle rocking. A second digestion buffer containing 25 mg/ml Collagenase I, 25 mg/ml Collagenase II, 50mg/ml Collagenase IV, and 25 mg/ml Dispase II in PBS was added, and tissues further dissociated for 2.5 hours at 37 °C with gentle rocking. Enzymatic digestion was quenched by addition of 10% FCS in PBS on ice. The cell suspensions were passaged through a 40µm cell strainer (Greiner Bio One) to remove cell debris and buffer residue. Cell suspensions were gently centrifuged at 350g for 7 min at 4°C. Cells were resuspended in PBS containing 0,04% FCS, 2 mM EDTA and propidium iodide (PI) was added to final concentration of 1 µg/ml prior to FACS sorting. 10x 3'-scRNA-seq ver. 3.1 (10X Genomics® User guide Rev.C and D) 10x 3'-scRNA-seq ver. 3.1 (10X Genomics® User guide Rev.C and D)
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2024-01-31T16:00:08Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2024-01-31T16:00:08Z
attributes.tag: External Id
attributes.value: SAMEA112953938
attributes.tag: INSDC center name
attributes.value: German Cancer Research Center (DKFZ), Division of Somatic Evolution and Early Detection
attributes.tag: INSDC first public
attributes.value: 2024-01-31T16:00:08Z
attributes.tag: INSDC last update
attributes.value: 2024-01-31T16:00:08Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-12889:HA15M01_od-01
attributes.tag: age
attributes.value: 15
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: common name
attributes.value: house mouse
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: normal
attributes.tag: genotype
attributes.value: wild type genotype
attributes.tag: individual
attributes.value: Ind1
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: oviduct
attributes.tag: sample name
attributes.value: E-MTAB-12889:HA15M01_od-01
attributes.tag: scientific_name
attributes.value: Mus musculus
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: C57BL/6J