title: dsGFP_2
organism: Anopheles gambiae
description: Protocols: Three-day old female females Anopheles coluzzii mosquitoes were injected with dsENH07 or dsGFP, and after 2 days, total RNAs from three biological replicates were extracted and sequenced. For each biological replicate, pools of ~10 mosquitoes were extracted per treatment group. Three-day-old cold-anaesthetized mosquito females were injected using the NanoJect II (Drummond) either with 1.5mg of an equal quantity of ds1, ds2 and ds3 (500 ng each per mosquito) or with 1.5mg of dsGFP for the control group as described above. Two days post-injection, for each condition, total RNA was extracted from a pool of 10 mosquitoes with TRI Reagent (Molecular Research Center, Inc.). The use of 3dsRNA was to optimize the targeting and silencing of the ENH07 eRNA. Three independent experiments were performed and the extracted total RNA from each condition and each experiment were submitted to transcriptome analysis.  total RNA was extracted from a pool of 10 mosquitoes with TRI Reagent (Molecular Research Center, Inc.) The libraries were prepared with the illumina TruSeq Stranded mRNA kit
attributes.tag: ENA first public
attributes.value: 2024-10-13
attributes.tag: INSDC center name
attributes.value: Genetics and Genomics of Insect Vectors Unit, Department of Parasites and Insect Vectors, Institut Pasteur, Université de Paris Cité, CNRS UMR2000, F-75015 Paris, France
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-14478:dsGFP_2
attributes.tag: age
attributes.value: 3
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: African malaria mosquito
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: genotype
attributes.value: WT
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: individual
attributes.value: Pool of 10 mosquitoes
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: whole body
attributes.tag: sample name
attributes.value: E-MTAB-14478:dsGFP_2
attributes.tag: scientific_name
attributes.value: Anopheles gambiae
attributes.tag: sex
attributes.value: female
attributes.tag: strain
attributes.value: M