title: Neurons_CTL2_D31
organism: Homo sapiens
description: Protocols: MAN7 hESCs were grown on Vitronectin (ThermoFisher Scientific). At the start of the protocol (adapted from ￼65 on Day 0 cells were plated on Matrigel (Corning) and cultured using mTESR1 medium (StemCell Technologies) supplemented by FGF2 (10ng/ml). On Day 1 neuronal differentiation was undertaken using neuronal induction medium supplemented by SMAD signaling inhibitors, Noggin and SD43154. On Day 10 cells were passaged and plated on a poly-l-ornithine and laminin (Sigma) substrate for neuronal maintenance and maturation. On Day 12 small-elongated cells generate rosette structures resembling early neuronal tubes and were propagated in culture using FGF2 (20ng/ml) for about 3 days. On Day 15-18 cells were passaged using Dispase (StemCell Technologies) and plated on a poly-l-ornithine and laminin substrate for neuronal maturation and cultured up to Day 31. Cells used for RNAseq were sorted for CD44- CD24+ and CD184+ for the progenitor stage at Day 18 and for CD44- CD24+ and CD184-(BD Bioscience) for neurons at Day 31. RNeasy Mini Kit - QIAGEN columns truseq stranded total rna with ribozero
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2022-12-25
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2022-12-25
attributes.tag: External Id
attributes.value: SAMEA8395595
attributes.tag: INSDC center alias
attributes.value: Istituto Europeo di Oncologia IEO IRCSS S.r.l.
attributes.tag: INSDC center name
attributes.value: Istituto Europeo di Oncologia IEO IRCSS S.r.l.
attributes.tag: INSDC first public
attributes.value: 2022-12-25T00:20:49Z
attributes.tag: INSDC last update
attributes.value: 2022-12-25T00:20:49Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-10244:Neurons_CTL2_D31
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: DS_CTL2
attributes.tag: cell_type
attributes.value: neuron
attributes.tag: common name
attributes.value: human
attributes.tag: disease
attributes.value: normal
attributes.tag: progenitor cell type
attributes.value: embryonic stem cell
attributes.tag: sample name
attributes.value: E-MTAB-10244:Neurons_CTL2_D31
attributes.tag: sex
attributes.value: not available