title: MA10_m4_brain
organism: Severe acute respiratory syndrome coronavirus 2
description: BALB/c mice were challenged intranasally with 7x10^3 plaque forming units (PFU) of SARS-CoV-2/MA10 and euthanized at 5-days post infection. Whole lung and brain tissue was dissected and RNA extracted for viral whole genome sequencing.
attributes.tag: collected_by
attributes.value: Laboratory of Justin Richner at the University of Illinois at Chicago
attributes.tag: collection_date
attributes.value: 2021-05-03
attributes.tag: geo_loc_name
attributes.value: USA: Illinois, Chicago
attributes.tag: host
attributes.value: Homo sapiens
attributes.tag: host_disease
attributes.value: COVID-19
attributes.tag: isolate
attributes.value: SARS-CoV-2/MA10 (mouse-adapted)
attributes.tag: isolation_source
attributes.value: Obtained from BEI Resources (NR-55329)
attributes.tag: collection_method
attributes.value: Whole tissue dissection
attributes.tag: date_of_sars_cov_2_vaccination
attributes.value: none
attributes.tag: exposure_event
attributes.value: BALB/c (Jax Strain #00651) mice were challenged intranasally with 7*10^3 plaque-forming units (PFU) of SARS-CoV-2/MA10 and tissue was harvested at 5 days post-infection.
attributes.tag: host_anatomical_part
attributes.value: brain
attributes.tag: passage_method
attributes.value: Virus was propagated on Vero-E6 cells (ATCC) for one passage prior to use.
attributes.tag: passage_number
attributes.value: 1
attributes.tag: prior_sars_cov_2_vaccination
attributes.value: no
attributes.tag: sars_cov_2_diag_gene_name_1
attributes.value: N (orf9)
attributes.tag: sars_cov_2_diag_pcr_ct_value_1
attributes.value: 22.628067016601562
attributes.tag: sequenced_by
attributes.value: Center for Pathogen Genomics and Microbial Evolution (CPGME) at Northwestern University, Chicago, Illinois
attributes.tag: vaccine_received
attributes.value: None.
attributes.tag: BioSampleModel
attributes.value: SARS-CoV-2: clinical or host-associated