title: Control-T3
organism: Homo sapiens
description: Protocols: Cells were collected in the TRIzol reagent and lysed by repetitive pipetting. Cells were incubated at 37°C, 5% CO2, and 95% humidity. Empty vector control in Caco-2 cells by lentiviral vector-based transduction. Qiagen RNeasyMini Kit according to the manufacturer instructions. Total RNA was isolated and sent for RNAseq analysis The RNA quality assessment is done using RNA screen Tape system. RNA concentration is determined in a Qubit.3 Fluorometer using the Qubit RNA BR assay kit. The fragmented mRNAs were primed with random hexamers, and then reverse transcribed to form cDNA. The cDNA fragments obtained were cleaned up by using AM pure beads. The DNA underwent end repair wherein the mix converts the overhangs resulting from fragmentation into blunt ends. The samples were further purified using AMPure beads and The DNA was then enriched by PCR using illumina universal primer and sample-specific octamer primers. The amplified products were cleaned up by using AM pure beads and the final mRNA library was eluted.
attributes.tag: ENA-FIRST-PUBLIC
attributes.value: 2024-02-12T00:25:51Z
attributes.tag: ENA-LAST-UPDATE
attributes.value: 2024-02-12T00:25:51Z
attributes.tag: External Id
attributes.value: SAMEA115162449
attributes.tag: INSDC center name
attributes.value: Department of Developmental Biology and Genetics
attributes.tag: INSDC first public
attributes.value: 2024-02-12T00:25:51Z
attributes.tag: INSDC last update
attributes.value: 2024-02-12T00:25:51Z
attributes.tag: INSDC status
attributes.value: public
attributes.tag: Submitter Id
attributes.value: E-MTAB-13746:Control-T3
attributes.tag: broker name
attributes.value: ArrayExpress
attributes.tag: cell_line
attributes.value: Caco-2
attributes.tag: cell_type
attributes.value: epithelial cell
attributes.tag: collection date
attributes.value: not collected
attributes.tag: common name
attributes.value: human
attributes.tag: developmental stage
attributes.value: adult
attributes.tag: disease
attributes.value: colorectal adenocarcinoma
attributes.tag: genotype
attributes.value: empty vector
attributes.tag: geographic location (country and/or sea)
attributes.value: not collected
attributes.tag: isolate
attributes.value: not applicable
attributes.tag: organism part
attributes.value: large intestine
attributes.tag: sample name
attributes.value: E-MTAB-13746:Control-T3
attributes.tag: scientific_name
attributes.value: Homo sapiens